CN104823692A - Ophiocordyceps sinensis wheat - Google Patents
Ophiocordyceps sinensis wheat Download PDFInfo
- Publication number
- CN104823692A CN104823692A CN201510126376.1A CN201510126376A CN104823692A CN 104823692 A CN104823692 A CN 104823692A CN 201510126376 A CN201510126376 A CN 201510126376A CN 104823692 A CN104823692 A CN 104823692A
- Authority
- CN
- China
- Prior art keywords
- wheat
- cordyceps militaris
- cordyceps
- fermentation
- sinensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to ophiocordyceps sinensis wheat. Wheat is used as a culture medium, ophiocordyceps sinensis and cordyceps militaris are used for double-fungus mixture and solid state fermentation, and the ophiocordyceps sinensis wheat belongs to the technical field of bioengineering. According to the invention, the wheat obtained through double-fungus bioconversion has the efficacies of health preservation and health care, and has favorable application prospects.
Description
Technical field
Adopting Cordyceps sinensis (Ophiocordyceps sinensis) and Cordyceps militaris (Cordyceps militaris), take wheat as medium, carries out two bacterium mixed solid fermentation, belongs to technical field of bioengineering.Utilizing bacterial strain Cordyceps militaris preserving number to be CICC 14013, bacterial strain Ophiocordyceps sinensis preserving number is CICC 14088, is produced obtain nutrition and health care wheat by two bacterium mixed solid fermentation.
Background technology
Cordyceps (Cordyceps) is under the jurisdiction of mycota (Fungi), Ascomycota (Ascomycota), Ascomycetes (Ascomycetes), excrement shell bacterium subclass (Sordariomycetidae), Hypocreales (Hypocreales), Clavicipitaceae (Clavicipitaceae).Cordyceps sinensis fungus great majority are medicinal fungis, wherein Cordyceps sinensis (Ophiocordyceps sinensis) is generally well known, applicating history is also remote, and the application study of Cordyceps militaris (Cordyceps militaris) is the most deep.
Cordyceps sinensis is recorded in 710 years Christian eras " moon king medicine is examined " the earliest according to textual research, records first appeared in Qing Dynasty Wu Yi Lip river " new compilation of materia medica " (1757 Christian eras) with medicine, thinks its " sweet flat; to protect lung kidney-nourishing; hemostasis and phlegm, phthisical cough, control diaphragm disease all good ".Cordyceps sinensis is China's tradition rare Chinese medicine, and it and ginseng, pilose antler are described as Chinese medicine Triratna together.It has mend and not high, temperature and not fiery, grow and oiliness pharmacodynamic profile, have more negative and positive with the unique effects of mending, make it become nourishing panacea, be described as " kings of hundred medicines " by physician.
Cordyceps militaris is one of Chinese medicinal material of being of great rarity of China, and its medical value is on the books very early, and Li Shizhen (1518-1593 A.D.) (1518) is pointed out in Compendium of Material Medica, and cicada fungus can cure mainly " children's hangs in sky frightened epilepsy, the morbid night crying of babies, palpitaition ".Cordyceps militaris is easy to large-scale artificial and cultivates, and its active component and pharmacological action are similar to Cordyceps sinensis again.
The current grain raw material that Cordyceps sinensis or Cordyceps militaris is applied to ferments by wide coverage.Such as: patent 200610117997.4 adopts Cordyceps militaris industrialization to cultivate discarded object and makes rice flour product, Chinese caterpillar fungus is pulverized rear mixing with the careless highland barley fried and makes health products by patent 200710050135.9, patent 200710166278.6 adopts wheat to cultivate fruiting bodies of cordyceps militaris, patent 200810068734.8 adopts adopting red yeast rice synergistic to ferment thus improves the output of fruiting bodies of cordyceps militaris and cordycepin, patent 200910218363.1 discloses a kind of method being improved primary medicinal component by Poria cocos and Cordyceps militaris solid co-fermentation, patent 201110055425.9 utilizes solid state fermentation to produce Cordyceps militaris fermentation rice, patent 201110348239.4 take lotus seeds as matrix, carries out the solid state fermentation of Cordyceps militaris or winter grass summer grass, patent 201110064459.4 discloses a kind of preparation method of Cordyceps militaris paddy food, patent 201310150267.4 and 201310151228.6 respectively with soya bean and peanut for matrix, carry out solid state fermentation after inoculation Cordyceps militaris, but need in process to add insect enzymolysis liquid, cause cost and difficult quality to control, patent 201210064068.7 discloses a kind of with glossy ganoderma, Phellinus etc. can the mushroom entity of efficient cellulase-producing as medium major ingredient, grain raw material is auxiliary material, inoculate into Cordyceps militaris, Cordyceps sinensis, mushroom, Hericium erinaceus, the bacterial classifications such as acupuncture needle aunt carry out Mixed culture, make health products or additive, but this complex process, edible and medical fungi fruit body is expensive, and after autoclaving, destroy the active ingredient of edible and medical fungi fruit body, complicated component and uncontrollable after other strain fermentation again, the strain combination of various complexity also will cause the instability of end product quality.
In sum, the solid ferment process of current Cordyceps militaris or Cordyceps sinensis all can add mineral salt, insect hydrolyzate or other medicinal ingredient, to improving Chinese caterpillar fungus thalline or cordycepin output and drug effect.Different additives can cause complicated component and import external pollution source, and is not easy to set up quality index system.In addition, the solid ferment process of Cordyceps militaris often using cordycepin (Cordycepin) as important quality index.Cordycepin, is also referred to as 3'-Deoxyadenosine (3-deoxyadenosine), and since self-discovery, its pharmacological action is constantly made known.Personnel study discovery after deliberation, and cordycepin has immunological regulation, antitumor, anti-leucocyte, the multiple pharmacological effect such as antimycotic, antiviral.What is interesting is, cordycepin is mainly stored in Cordyceps militaris, and content in Cordyceps sinensis is atomic, but the disappearance of cordycepin does not affect the remarkable efficacy of Cordyceps sinensis.Therefore, these two kinds of effects of Cordyceps sinensis and Cordyceps militaris are similar, but the incomplete same bacterial classification of composition has complementarity.
Summary of the invention
Based on current situation, the present invention take wheat as medium, when not adding other additive any, only wheat is carried out expanding treatment, after sterilizing, Simultaneous vaccination Cordyceps sinensis and Cordyceps militaris carry out Mixed culture, finally obtain the health care wheat of high cordycepin content and high bioactivity.The method is implemented simple, quality controllable, has wide application and development prospect.
The present invention's Cordyceps strain Ophiocordyceps sinensis preserving number used is CICC14088, and cordyceps militaris link bacterial strain Cordyceps militari preserving number is CICC 14013.
Technical scheme of the present invention is as follows:
(1) part wheat adopts the twin-screw extruder containing three sections to process.1st section is preheating zone, and serviceability temperature is 40-60 DEG C, and the 2nd district is traffic zone, and temperature is 120-160 DEG C, and the 3rd district is slaking district, and temperature is 140-180 DEG C.The water content of expanded front wheat is 10%-20%.
(2) part wheat mechanical crushing is 0.1-2mm to particle diameter.
(3) mixed with certain proportion with the wheat of mechanical crushing by the wheat of extrusion, the wheat proportion of extrusion is 1-100%.Keep the skin wet after mixing and make water content reach 20%-70%, 121 DEG C of insulations sterilizing in 20 minutes.After cooling, access Cordyceps sinensis, inoculum concentration is 1-10%.Meanwhile, access Cordyceps militaris, inoculum concentration is 1-10%.Initial pH and fermentation process pH is nature, and fermentation temperature is 15-28 DEG C, and envionmental humidity is 60%-100%, and fermentation period is 5-25 days.The content of final cordycepin reaches as high as 8.1%.
The Cordyceps sinensis that the present invention is used and Cordyceps militaris spawn preservation slant medium formula are: glucose 20g/l, potato juice 200g/l, agar 20g/l, pH nature.
The seed culture based formulas of Cordyceps sinensis of the present invention and Cordyceps militaris is: glucose 20g/l, potato juice 200g/l, pH nature.After slant strains is inoculated into the triangular flask that seed culture medium is housed, the incubation time of seed is 96 hours, and the cultivation temperature of seed is 25 DEG C.
Cordycepin content adopts liquid chromatography for measuring.Assay method refers to: Wei Huiping, Ye little Li, Zhang Huaying, Li Xuegang, Zhong Yunjun, with the research of Cordyceps militaris solid fermentation method High-efficient Production cordycepin, and CHINA JOURNAL OF CHINESE MATERIA MEDICA, 1998,33 (19), 2159-2162.
Beneficial effect of the present invention: by expanded, makes wheaten starch gelatinization and aleuronat sex change at short notice.Extruded wheat ferments after mixing with mechanical crushing wheat, the nutriment making thalline to utilize puffing process to produce fast on the one hand, on the other hand mechanical crushing wheat is the equal of that solid state fermentation carrier makes fermentation substrate keep loose favourable thalli growth, and when thalli growth is vigorous, thalline produces a large amount of extracellular enzyme and can decompose further again and utilize mechanical crushing wheat.This process, without the need to adding other material, ensures that the quality height of fermented product is controlled.The common fermentation of Cordyceps sinensis and Cordyceps militaris two kinds of similar bacterial classifications of character, on the one hand the tunning of similar quality bacterial classification can not change overall pharmacology pharmacy proterties, can be in harmonious proportion two kinds of strain fermentation products defect separately on the other hand.The method simple possible, is easy to accomplish scale production, is with a wide range of applications.
Embodiment
Embodiment 1
Adopt the DS56-III type twin-screw extruder process wheat that Jinan Ju Xian plant equipment Co., Ltd produces, wheat used is commercially available, and measuring its moisture content through moisture teller is 14.2%.Setting charging rate is 120kg/h.Table 1 lists temperature combination and the puffed degree of three sections.When the temperature in preheating zone, traffic zone, slaking district is respectively 60,160,190 DEG C, the puffed degree of wheat is the highest as can be seen from Table 1, under this temperature condition, in wheat, supplement a small amount of sterile water further or wheat is dried further, investigating puffed degree during different moisture content.As can be seen from Table 2, moisture content is that expansion effect is best when about 14-18%, therefore can carry out expanded by direct Commercial wheat.
Table 1: the different each section different temperatures combination of extruder obtains the puffed degree of extruded wheat
Table 2: the puffed degree after different water cut wheat puffing
| Moisture content % | 10.6 | 12.1 | 14.2 | 16.2 | 17.1 | 18.3 | 19.7 |
| Puffed degree | 1.5 | 1.8 | 2.4 | 2.4 | 2.3 | 2.3 | 2.1 |
Embodiment 2
Be inoculated into by aweto strain and be equipped with in the 250ml triangular flask of 50ml medium, medium consists of: glucose 20g/l, and potato juice 200g/l, pH nature, 25 DEG C, shaking speed 150 turns/min, cultivates 96 hours, as inoculation seed.
Be inoculated into by Cordyceps militaris spawn and be equipped with in the 250ml triangular flask of 50ml medium, medium consists of: glucose 20g/l, and potato juice 200g/l, pH nature, 25 DEG C, shaking speed 150 turns/min, cultivates 96 hours, as inoculation seed.
Get puffed degree be 2.5 extruded wheat and mechanical crushing degree be 1mm wheat mixes, amount to 50g, adding distil water 40g again, put into 500ml triangular flask, 121 DEG C of sterilizing 20min, inoculate after cooling into Cordyceps sinensis and each 5ml of Cordyceps militaris seed liquor, control environment humidity 90% in constant temperature and humidity incubator, temperature is 25 DEG C, ferments and measures cordycepin content after 15 days.Cordycepin output under different condition combination is as shown in table 3.When extruded wheat and mechanical crushing wheat ratio are 3:2, cordycepin produces the highest as can be seen from Table 3, can reach 6.4%.
Table 3: solid state fermentation cordycepin content under extruded wheat and mechanical crushing degree different proportion
| Extruded wheat weight (g) | Mechanical crushing wheat weight (g) | Cordycepin content (%) |
| 50 | 0 | 2.8 |
| 1 | 49 | 3.1 |
| 25 | 25 | 3.8 |
| 10 | 40 | 3.2 |
| 40 | 10 | 3.3 |
| 20 | 30 | 5.2 |
| 30 | 20 | 6.4 |
Embodiment 3
Get puffed degree be 2.5 extruded wheat 30g and mechanical crushing degree be 2mm wheat 20g mixes, adding distil water 40g again, put into 500ml triangular flask, 121 DEG C of sterilizing 20min, inoculate into cultured Cordyceps sinensis and each 5ml of Cordyceps militaris seed liquor after cooling, control environment humidity 80% in constant temperature and humidity incubator, and temperature is 15 DEG C, ferment 20 days afterwards measure cordycepin content be 5.3%.
Embodiment 4
Get puffed degree be 2.5 extruded wheat 30g and mechanical crushing degree be 0.5mm wheat 20g mixes, adding distil water 50g again, put into 500ml triangular flask, 121 DEG C of sterilizing 20min, inoculate into cultured Cordyceps sinensis and each 6ml of Cordyceps militaris seed liquor after cooling, control environment humidity 90% in constant temperature and humidity incubator, and temperature is 20 DEG C, ferment 5 days afterwards measure cordycepin content be 1.8%.
Embodiment 5
Get puffed degree be 2.5 extruded wheat 30g and mechanical crushing degree be 1mm wheat 20g mixes, adding distil water 45g again, put into 500ml triangular flask, 121 DEG C of sterilizing 20min, inoculate into cultured Cordyceps sinensis seed liquor 5ml after cooling, control environment humidity 90% in constant temperature and humidity incubator, and temperature is 20 DEG C, ferment 25 days afterwards measure cordycepin content be 0.03%.The fermentation product that this process obtains is designated as sample 1.
Get puffed degree be 2.5 extruded wheat 30g and mechanical crushing degree be 1mm wheat 20g mixes, adding distil water 45g again, put into 500ml triangular flask, 121 DEG C of sterilizing 20min, inoculate into cultured Cordyceps militaris seed liquor 5ml after cooling, control environment humidity 90% in constant temperature and humidity incubator, and temperature is 20 DEG C, ferment 25 days afterwards measure cordycepin content be 7.5%.The fermentation product that this process obtains is designated as sample 2.
Get puffed degree be 2.5 extruded wheat 30g and mechanical crushing degree be 1mm wheat 20g mixes, adding distil water 40g again, put into 500ml triangular flask, 121 DEG C of sterilizing 20min, inoculate into cultured Cordyceps sinensis and Cordyceps militaris seed liquor 5ml after cooling, control environment humidity 90% in constant temperature and humidity incubator, and temperature is 20 DEG C, ferment 25 days afterwards measure cordycepin content be 8.1%.The fermentation product that this process obtains is designated as sample 3.
Get the Kunming mouse 40 purchased from Shanghai Slac Experimental Animal Co., Ltd., Individual Quality 18-22g, male and female half and half.Be divided into 4 groups at random, often organize 10, be respectively sample 1,2,3 administration group and blank group.In first 7 days of experiment, every day, gavage gave different solid state fermentation samples, and the sample size of every mouse gavage is 0.5g.Blank group gavage to be unfermentable puffed degree be 2.5 0.5g wheat.7th day, every mouse peritoneal injected 2% starch 2ml, after 24h, every mouse peritoneal injects 2% chicken red blood cell 1ml, after 30min, broken end, Intraperitoneal injection 0.2ml heparin, get peritoneal fluid smear, Rui Shi-Ji's nurse Sa dyeing 1min, salt solution gradient washes, oily sem observation phagocytic percentage, phagocytic index, get spleen, thymus gland is weighed, calculate spleen index, thymus index.
Spleen index=spleen average quality (mg)/average body quality (g).
Thymus index=thymus gland average quality (mg)/average body quality (g).
The cell number of the quantity of phagocytic index=engulf/engulf.
The phagocyte number of the cell number of phagocytic rate=engulf/total.
Spleen index is listed, thymus index, phagocytic index and phagocytic rate in table 4.
Table 4: different fermentations sample is on the impact of mouse spleen, thymic factor D injection, Peritoneal Macrophage Phagocytosis
| Group | Thymus index | Spleen index | Phagocytic rate (%) | Phagocytic index |
| Blank group | 1.82±0.23 | 2.91±0.22 | 47.3±5.43 | 1.9±0.1 |
| 1 group, sample | 2.30±0.44 | 4.12±0.36 | 57.3±10.1 | 2.2±0.2 |
| 2 groups, sample | 2.18±0.34 | 3.83±0.23 | 62.4±11.4 | 2.1±0.2 |
| 3 groups, sample | 3.29±0.47 | 5.93±0.43 | 86.2±10.3 | 3.1±0.4 |
Index and spleen index and thymus index are the important parameters directly reflecting Immunologic States, and sample 1-3 group all significantly can promote the growth of mouse spleen and thymus gland relative to blank group as can be seen from Table 4, the immune level effect of being significantly improved.The Be very effective that worm winter, worm summer and Cordyceps militaris carry out 3 groups, the sample of mixed culture fermentation is better than 1 group, the sample of Cordyceps sinensis list strain fermentation and 2 groups, the sample of the single strain fermentation of Cordyceps militaris.
1 group, sample fermenting compared to single bacterium of worm winter, worm summer and Cordyceps militaris 3 groups, sample carrying out mixed culture fermentation and 2 groups, sample significantly can promote the phagocytic function of Turnover of Mouse Peritoneal Macrophages as can be seen from Table 4, the anti-infective of body and anti-tumor capacity can be improved, strengthen the immunologic function of body, stable state of keeping fit.
Claims (2)
1. the present invention adopts wheat to be matrix, carries out Cordyceps sinensis and Cordyceps militaris mixed solid fermentation.
2. mixed solid fermentation according to claim 1, is characterized in that part wheat need carry out expanding treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510126376.1A CN104823692A (en) | 2015-03-20 | 2015-03-20 | Ophiocordyceps sinensis wheat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510126376.1A CN104823692A (en) | 2015-03-20 | 2015-03-20 | Ophiocordyceps sinensis wheat |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104823692A true CN104823692A (en) | 2015-08-12 |
Family
ID=53802228
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510126376.1A Pending CN104823692A (en) | 2015-03-20 | 2015-03-20 | Ophiocordyceps sinensis wheat |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104823692A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109749908A (en) * | 2019-03-21 | 2019-05-14 | 安徽农业大学 | A kind of preparation method of yellow big tea wine song |
| CN115735675A (en) * | 2022-12-06 | 2023-03-07 | 天水众安生物科技有限责任公司 | Cordyceps militaris culture medium based on extrusion puffing technology and preparation method and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1243678A (en) * | 1998-08-01 | 2000-02-09 | 刘瑞林 | Technology for producing instant food by fungus cultivation |
| CN1723758A (en) * | 2005-07-12 | 2006-01-25 | 天津师范大学 | Cordyceps sinensis mycelium and its prepn. method |
| CN101066090A (en) * | 2007-05-28 | 2007-11-07 | 李治德 | Aweto activated reinforced immunological feed additive and its production process |
| CN101171968A (en) * | 2006-10-30 | 2008-05-07 | 陈淑德 | Cereal solid-state fermentation chinese caterpillar fungus and ganoderma lucidum product, and preparing method thereof |
| CN101971764A (en) * | 2010-03-22 | 2011-02-16 | 陈顺志 | Industrial energy-saving and low-carbon method for cultivating cordyceps militaris |
| CN102388795A (en) * | 2011-11-08 | 2012-03-28 | 付湘晋 | Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds |
| CN102599481A (en) * | 2012-03-12 | 2012-07-25 | 杨毅 | Method for preparing solid fermentation mixed edible medicinal fungus sporocarp |
| CN103271281A (en) * | 2013-06-09 | 2013-09-04 | 食品行业生产力促进中心 | Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation |
-
2015
- 2015-03-20 CN CN201510126376.1A patent/CN104823692A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1243678A (en) * | 1998-08-01 | 2000-02-09 | 刘瑞林 | Technology for producing instant food by fungus cultivation |
| CN1723758A (en) * | 2005-07-12 | 2006-01-25 | 天津师范大学 | Cordyceps sinensis mycelium and its prepn. method |
| CN101171968A (en) * | 2006-10-30 | 2008-05-07 | 陈淑德 | Cereal solid-state fermentation chinese caterpillar fungus and ganoderma lucidum product, and preparing method thereof |
| CN101066090A (en) * | 2007-05-28 | 2007-11-07 | 李治德 | Aweto activated reinforced immunological feed additive and its production process |
| CN101971764A (en) * | 2010-03-22 | 2011-02-16 | 陈顺志 | Industrial energy-saving and low-carbon method for cultivating cordyceps militaris |
| CN102388795A (en) * | 2011-11-08 | 2012-03-28 | 付湘晋 | Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds |
| CN102599481A (en) * | 2012-03-12 | 2012-07-25 | 杨毅 | Method for preparing solid fermentation mixed edible medicinal fungus sporocarp |
| CN103271281A (en) * | 2013-06-09 | 2013-09-04 | 食品行业生产力促进中心 | Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109749908A (en) * | 2019-03-21 | 2019-05-14 | 安徽农业大学 | A kind of preparation method of yellow big tea wine song |
| CN109749908B (en) * | 2019-03-21 | 2021-12-17 | 安徽农业大学 | Preparation method of yellow big tea distiller's yeast |
| CN115735675A (en) * | 2022-12-06 | 2023-03-07 | 天水众安生物科技有限责任公司 | Cordyceps militaris culture medium based on extrusion puffing technology and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104718987A (en) | Cordyceps sinensis chenopodium quinoa | |
| CN101543285B (en) | Wheat-bran dietary fiber composite functional fungus powder product and preparation process thereof | |
| CN103483021B (en) | Method for preparing liquid fertilizer by converting Cordyceps fermentation residual liquid with EM (effective microorganism) flora | |
| CN104718986A (en) | Cordyceps sinensis Chinese yam | |
| CN104893992A (en) | Submerged fermentation method for ganoderma lucidum | |
| CN105147715B (en) | A kind of new application of Paecilomyces hepiali chen neutrality exocellular polysaccharide | |
| CN104823693A (en) | Cordyceps sinensis soybeans | |
| CN104757427A (en) | Chinese caterpillar fungus coix seeds | |
| CN104823692A (en) | Ophiocordyceps sinensis wheat | |
| CN104823690A (en) | Cordyceps sinensis and cordyceps millitaris buckwheat | |
| CN104686199A (en) | Cordyceps sinensis highland barley | |
| CN104719782A (en) | Cordyceps sinensis mung beans | |
| CN103864504A (en) | Solid fermentation matrix for culturing edible and medicinal fungus, and preparation method and application thereof | |
| CN104718989A (en) | Cordyceps sinensis dasheen | |
| CN104757454A (en) | Chinese caterpillar fungus lotus root | |
| TWI385248B (en) | A formula of culturing medium for cordyceps spp. | |
| CN109258296B (en) | Semi-continuous submerged fermentation process for high-yield auricularia auricula polysaccharide | |
| CN104855135A (en) | Cordyceps sinensis broomcorn product | |
| CN104718985A (en) | Cordyceps sinensis rice | |
| CN104823696A (en) | Cordyceps red bean | |
| CN104718988A (en) | Cordyceps sinensis lotus seeds | |
| CN104823689A (en) | Cordyceps millet | |
| CN104824588A (en) | Cordyceps sinensis potatoes | |
| CN104686202A (en) | Cordyceps sinensis sweet potatoes | |
| CN103555630B (en) | Gordoniasinensis, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150812 |