CN115735675A - Cordyceps militaris culture medium based on extrusion puffing technology and preparation method and application thereof - Google Patents
Cordyceps militaris culture medium based on extrusion puffing technology and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a cordyceps militaris culture medium based on an extrusion expansion technology, and a preparation method and application thereof, and belongs to the technical field of artificial cultivation of cordyceps militaris. The preparation method of the cordyceps militaris culture medium based on the extrusion puffing technology comprises the following steps: (1) Crushing the grain raw material, adjusting the water content, and extruding and puffing to obtain an extruded and puffed material; (2) crushing the bean raw materials to obtain raw materials; (3) And mixing the extruded and expanded material with the raw material, sterilizing, and adding water to obtain the cordyceps militaris culture medium. The invention prepares the extruded and expanded material and the raw material into the culture medium according to the proportion, not only utilizes the advantage of rapid yield increase of the expanded material, but also overcomes the problem of insufficient growth aftereffect of the cordyceps militaris caused by singly using the extruded and expanded material by adding the raw material. And hot sterile water is used for filling, so that the dissolution of the nutritional ingredients of the extruded and expanded material and the raw material is promoted, and the biotransformation rate of the cordyceps militaris is improved.
Description
Technical Field
The invention relates to the technical field of artificial cultivation of cordyceps militaris, in particular to a cordyceps militaris culture medium based on an extrusion swelling technology, and a preparation method and application thereof.
Background
In the artificial cultivation of cordyceps militaris, grains such as rice, wheat and oat are generally used as cultivation substrates, and the grain crops can be used for cultivating the cordyceps militaris by curing. The grain ripening mainly has two functions, one is gelatinization of starch, protein and the likeThe denaturation of the texture components and the killing of microorganisms carried by the grains are beneficial to the fermentation. The grain aging technology comprises steaming (at high temperature and high pressure or normal temperature and normal temperature), steam explosion, parching, baking (at high temperature or low temperature), puffing (including frying puffing, extrusion puffing, airflow puffing, microwave puffing and CO puffing 2 Puffing, etc.). The cooking technology is widely applied and is characterized in that after the cordyceps militaris is cultivated, the biotransformation rate is low and is about 100%.
The swelling material is used for cultivating the cordyceps militaris, and at present, the research reports at home and abroad are less. The patent (publication No. CN104584855A, published Japanese 20150506) indicates that the biological conversion rate after cultivation can reach 160-180% by adopting the extrusion and expansion technology. The patent (publication No. CN108373975A, published: 20180807) states that the puffed grain raw material is used as culture material, sugar, protein, vitamins, inorganic salt and the like are used as nutrient solution components, the culture material and the nutrient solution are bottled and inoculated, and then the low-temperature dark cultivation is adopted, and then the illumination is carried out to change the color to produce the grass, and the biotransformation rate after the cultivation can reach 180-200%. However, the above patent does not mention any specific preparation parameters of the expanded materials, and the technical scheme is not completely disclosed.
Meanwhile, in the implementation process of the patent, the problems of high hypha infiltration speed, early grass growth, insufficient late growth and easy premature fruiting body of cordyceps militaris exist. The reason is that: firstly, after the grains are extruded and puffed, the average molecular weight of starch is obviously reduced, and the starch is cracked to generate micromolecular substances such as maltodextrin and the like, so that the digestibility of the starch is increased; secondly, after the grains are extruded and puffed, the protein is denatured and degraded to form a flaky structure, so that the digestibility of the protein is improved. Based on the two main reasons, the cordyceps militaris hyphae can quickly exhaust the nutrient components of the swelling material, and the cordyceps militaris sporocarp can be supplied with the nutrient components by external addition at the later stage, so that the cordyceps militaris sporocarp with better quality can be obtained only by paying more labor cost and raw material cost. Based on the situation, a new cordyceps militaris culture medium needs to be developed urgently, normal growth of cordyceps militaris can be guaranteed, cost is reduced, and the method is more suitable for large-area popularization and application.
Disclosure of Invention
The invention aims to provide a cordyceps militaris culture medium based on an extrusion expansion technology, and a preparation method and application thereof. The invention prepares the extruded and expanded material and the raw material into the culture medium according to the proportion, not only utilizes the advantage of rapid yield increase of the expanded material, but also overcomes the problem of insufficient late growth strength of the cordyceps militaris caused by singly using the extruded and expanded material by adding the raw material. And hot sterile water is used for filling, so that the dissolution of the nutritional ingredients of the extruded and expanded material and the raw material is promoted, and the biotransformation rate of the cordyceps militaris is improved.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of a cordyceps militaris culture medium based on an extrusion expansion technology, which comprises the following steps:
(1) Crushing the grain raw material, adjusting the water content, extruding and puffing to obtain an extruded and puffed material;
(2) Pulverizing bean raw materials to obtain raw material;
(3) And mixing the extruded and expanded material with the raw material, sterilizing, and adding water to obtain the cordyceps militaris culture medium.
Preferably, the cereal raw material comprises one or more of wheat kernel, wheat grain, naked oat, barley, rice, corn and silkworm chrysalis meal; the bean material comprises one or more of semen glycines, semen Sojae Atricolor, semen Viciae Fabae, semen lablab album, semen Phaseoli Radiati and semen Phaseoli.
Preferably, the particle size of the crushed particles in the step (1) is 60-100 meshes, and the water content is 18-25%.
Preferably, the temperature of the extrusion puffing is 150-200 ℃.
Preferably, the extruded bulked feed is particles of-3 meshes to +7 meshes; the bulk density of the extruded and expanded material is 0.28-0.45 g/cm 3 。
Preferably, the raw meal is in the form of particles of-10 to +30 mesh.
Preferably, the mass ratio of the extruded puffed material to the raw material is 6:0.5 to 1.
Preferably, the temperature of the water is 80-95 ℃; the addition amount of the water is 2 to 4 times of the total mass of the extruded and expanded material and the raw material.
The invention also provides a cordyceps militaris culture medium prepared by the preparation method.
The invention also provides an application of the cordyceps militaris culture medium in improving the yield and the biotransformation rate of cordyceps militaris fruiting bodies.
The invention provides a cordyceps militaris culture medium based on an extrusion expansion technology, and a preparation method and application thereof. The invention utilizes the extrusion swelling material and the raw material as the culture medium to cultivate the cordyceps, the extrusion swelling material is used as the quick-acting nutrient source, the raw material is used as the slow-acting nutrient source, the advantage of quick yield increase of the swelling material is utilized, simultaneously, the raw material is added to overcome the problem of insufficient growth aftereffect of the cordyceps militaris caused by the independent use of the swelling material, and the two materials are used in combination to meet the nutrition required by the whole growth process of the cordyceps militaris. Through the control of the extrusion parameters, the bulk density of the expanded material is high, and the filling efficiency and the space utilization rate of the cultivation bottles are improved. Meanwhile, the invention utilizes the hot filling treatment technology, reduces the pollution risk, and promotes the nutrition of the extruded and expanded material and the raw material to be fully dissolved out, thereby improving the single yield of the cordyceps militaris and leading the biological conversion rate to reach 200-220%.
Drawings
FIG. 1 is a graph showing the relationship between different filling temperatures of sterilized water and the biotransformation efficiency in example 1;
FIG. 2 shows the material morphology of the extruded dilatant of example 2.
Detailed Description
The invention provides a preparation method of a cordyceps militaris culture medium based on an extrusion technology, which comprises the following steps:
(1) Crushing the grain raw material, adjusting the water content, extruding and puffing to obtain an extruded and puffed material;
(2) Crushing the bean raw materials to obtain raw materials;
(3) And mixing the extruded and expanded material with the raw material, sterilizing, and adding water to obtain the cordyceps militaris culture medium.
The invention obtains the extrusion swelling material by crushing the grain raw material, adjusting the water content and then extruding and swelling.
In the invention, the cereal raw material preferably comprises one or more of wheat kernel, wheat grain, naked oat, barley, rice, corn and silkworm chrysalis meal, and further preferably comprises wheat grain and silkworm chrysalis meal.
In the present invention, the pulverized particle size is preferably 60 to 100 mesh, and more preferably 80 mesh.
In the present invention, the water content is preferably 18 to 25%, more preferably 21.5%.
In the present invention, the extrusion-puffing is preferably carried out using a twin-screw extruder or a single-screw extruder, more preferably using a twin-screw extruder, and still more preferably using a DL 70-II twin-screw extruder.
In the present invention, the feed rate of the DL 70-II twin-screw extruder is preferably 15 to 25rpm, and more preferably 20rpm.
In the present invention, the screw rotation speed of the DL 70-II twin-screw extruder is preferably 140 to 160rpm, and more preferably 150rpm.
In the present invention, the temperature of the extrusion-expansion is preferably 150 to 200 ℃, and more preferably 160 ℃.
In the present invention, the extruded dilatant is preferably a particle of-3 mesh to +7 mesh, more preferably a particle of-3 mesh to +5 mesh.
In the present invention, the bulk density of the extruded dilatant is preferably from 0.28 to 0.45g/cm 3 More preferably 0.30g/cm 3 。
The invention pulverizes the bean raw material to obtain the raw material.
In the invention, the bean raw material preferably comprises one or more of soybeans, black beans, broad beans, hyacinth beans, mung beans and red beans, and further preferably black beans.
In the present invention, the pulverization is preferably performed using a pulverizer.
In the present invention, the rotation speed of the pulverizer is preferably 460 to 600rpm, and more preferably 520rpm.
In the present invention, the mesh size of the pulverizer is preferably 10 to 12 mesh, and more preferably 12 mesh.
In the present invention, the raw material is preferably a granule of-10 to +30 mesh.
The cordyceps militaris culture medium is obtained by mixing the extruded and expanded material with the raw material, sterilizing and adding water.
In the present invention, the mass ratio of the extruded expanded feed to the raw material is preferably 6:0.5 to 1, more preferably 6:0.5.
in the present invention, the sterilization is preferably performed by moist heat sterilization.
In the present invention, the temperature for the moist heat sterilization is preferably 120 to 130 ℃, and more preferably 121 ℃.
In the present invention, the time for the moist heat sterilization is preferably 25 to 35min, and more preferably 30min.
In the present invention, the water is preferably sterile water.
In the present invention, the temperature of the sterile water is preferably 80 to 95 ℃, and more preferably 85 ℃.
In the present invention, the addition amount of the sterile water is preferably 2 to 4 times of the total mass of the extruded puffed material and the raw material, and more preferably 2.5 times of the total mass of the extruded puffed material and the raw material.
After the cordyceps militaris culture medium is prepared, cordyceps militaris liquid strains are inoculated for cultivation.
In the invention, the cultivation method of the cordyceps militaris preferably comprises the steps of dark cultivation and then illumination cultivation.
In the present invention, the temperature of the dark culture is preferably 16 to 19 ℃ and more preferably 18 ℃.
In the present invention, the dark culture time is preferably 3 to 5 days, and more preferably 4 days.
In the present invention, the temperature of the light irradiation culture is preferably 16 to 22 ℃, and more preferably 20 ℃.
In the present invention, the light intensity of the above-mentioned light culture is preferably 50 to 1000Lux, and more preferably 800Lux.
In the invention, the cordyceps militaris is preferably harvested 50-60 days after inoculation, and is further preferably harvested 53 days after inoculation.
The invention also provides a cordyceps militaris culture medium prepared by the preparation method.
The invention also provides an application of the cordyceps militaris culture medium in improving the yield and the biotransformation rate of cordyceps militaris fruiting bodies.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are conventional products which are not indicated by manufacturers and are commercially available.
Example 1
The invention firstly verifies the influence of sterile water with different filling temperatures on the biological conversion rate of cordyceps militaris, and the operation is as follows:
selecting wheat kernels and corns as grain raw materials, and preparing the extruded and puffed material. Sieving the wheat kernels and the corns for removing impurities, and mixing the wheat kernels: corn =80:20, pulverizing, sieving with 60 mesh sieve, adjusting water content to 20%, extruding with 40 type single screw extruder at 185 deg.C with power of 7.5kw and throughput of 50kg/h to obtain extruded puffed material granules with particle size of-3 mesh to +7 mesh and bulk density of 0.42g/cm 3 . Selecting soybeans as bean raw materials, using a grinder with a screen mesh size of 12 meshes, setting the rotating speed to 520rpm, and screening after grinding to obtain raw material granules of minus 10 to plus 30 meshes. Mixing the extruded and expanded material and the raw material according to the proportion of 6:1, filling into bottles, performing damp-heat sterilization at 121 ℃ for 30min, respectively filling sterile water with the temperature of 20 ℃, 65 ℃, 75 ℃ and 85 ℃, wherein the addition amount of the sterile water is 2.5 times of the total mass of the extruded and expanded material and the raw material, standing, cooling and forming to obtain the cordyceps militaris culture medium.
Diluting 10 times of Cordyceps militaris strain with 10g/L sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, uniformly spraying the Cordyceps militaris liquid strain onto the surface of the prepared Cordyceps militaris culture medium, and inoculating 8mL of Cordyceps militaris culture medium per bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 4 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 20 ℃ under illumination of 800Lux. Harvesting 55 days after inoculation, weighing the harvested fresh cordyceps militaris fruiting bodies, and calculating the biotransformation rates of cordyceps militaris at different filling temperatures. The calculation method of the biotransformation efficiency is shown in formula (1). The results are shown in FIG. 1.
Biotransformation rate = (fresh weight of Cordyceps militaris fruiting body/dry weight of culture medium substrate) × 100% \ 8230; … formula (1)
As can be seen from FIG. 1, the biotransformation rate gradually increases with the increase of the filling temperature of the sterile water, which indicates that the increase of the filling temperature of the sterile water is beneficial to the growth of Cordyceps militaris.
Example 2
Selecting wheat grains and silkworm chrysalis powder as grain raw materials, and preparing the extruded and puffed material. Sieving the wheat grains and the silkworm chrysalis powder to remove impurities, and according to the wheat grains: silkworm chrysalis meal =95:5, crushing, sieving by 80 meshes, adjusting the water content to 21.5 percent, using a DL 70-II double-screw extruder, setting the feeding speed to be 20rpm, the screw rotating speed to be 150rpm and the extrusion temperature to be 160 ℃ to obtain extruded and expanded material particles with the particle size of-3 meshes to +5 meshes, wherein the bulk density of the extruded and expanded material is 0.30g/cm 3 . The material morphology of the extruded puff is shown in figure 2. Selecting black beans as a bean raw material, using a grinder with a screen mesh size of 12 meshes, setting the rotating speed to 520rpm, grinding and sieving to obtain raw material granules of-10 to +30 meshes. Mixing the extruded and expanded material and the raw material according to the proportion of 6:0.5, filling into a bottle, carrying out damp-heat sterilization at 121 ℃ for 30min, filling sterile water at 85 ℃, wherein the addition amount of the sterile water is 2.5 times of the total mass of the extruded and expanded material and the raw material, standing, cooling and forming to obtain the cordyceps militaris culture medium.
Diluting 10 times of Cordyceps militaris strain with 10g/L sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, uniformly spraying the Cordyceps militaris liquid strain onto the surface of the prepared Cordyceps militaris culture medium, and inoculating 8mL of Cordyceps militaris culture medium per bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 4 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 20 ℃ under illumination of 800Lux. Harvesting on 53 th day after inoculation, weighing the harvested fresh cordyceps militaris fruiting bodies, and calculating the biotransformation rate of cordyceps militaris according to the formula (1), wherein the biotransformation rate is 216.9%.
Example 3
Naked oats and rice are selected as grain raw materials, and the extrusion puffing material is prepared. Sieving naked oats and rice to remove impurities, and according to the weight of the naked oats: rice =90:10, crushing, sieving by 80 meshes, adjusting the water content to 18 percent, using a DL 70-II double-screw extruder, setting the feeding speed to be 20rpm, the screw rotating speed to be 160rpm and the extrusion temperature to be 165 ℃ to obtain extruded and expanded material particles with the particle size of-5 meshes to +7 meshes, wherein the bulk density of the extruded and expanded material is 0.42g/cm 3 . Selecting black beans as a bean raw material, using a grinder with a screen mesh size of 10 meshes, setting the rotating speed to 460rpm, grinding and then sieving to obtain raw material granules of-10 to +30 meshes. Mixing the extruded and expanded material and the raw material according to the proportion of 6:1, filling into bottles, performing damp-heat sterilization at 121 ℃ for 30min, filling sterile water at 65 ℃, wherein the addition amount of the sterile water is 3.5 times of the total mass of the extruded and expanded material and the raw material, standing, cooling and forming to obtain the cordyceps militaris culture medium.
Diluting 10 times of Cordyceps militaris strain with 10g/L sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, uniformly spraying the Cordyceps militaris liquid strain onto the surface of the prepared Cordyceps militaris culture medium, and inoculating 8mL of Cordyceps militaris culture medium per bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 4 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 20 ℃ under illumination of 800Lux. Harvesting on 50 th day after inoculation, weighing the harvested fresh cordyceps militaris fruiting bodies, and calculating the biotransformation rate of cordyceps militaris according to the formula (1), wherein the biotransformation rate is 200.8%.
Comparative example 1
The cordyceps militaris culture medium of the comparative example consists of single extruded and puffed materials, the raw material proportion is adjusted, the steps of raw material preparation and mixing are reduced, and other steps and parameters are consistent with those of the example 2. The specific operation is as follows:
selecting wheat grains, silkworm chrysalis meal and black beans as raw materials, sieving to remove impurities, and performing filtration according to the wheat grains: silkworm chrysalis meal: black bean =80:15:5, crushing, sieving by a sieve of 80 meshes, adjusting the water content to 21.5 percent, using a DL 70-II double screw extruder to set the feeding speed to be 20rpm, the screw rotating speed to be 150rpm and the extrusion temperature to be 160 ℃ to obtain extruded and expanded material particles with the particle size of minus 3 meshes to plus 5 meshes, wherein the bulk density of the extruded and expanded material is 0.30g/cm 3 . Bottling the prepared extruded and puffed material, performing damp-heat sterilization at 121 deg.C for 30min, bottling with 85 deg.C sterile water with addition amount of 2.5 times of total mass of the extruded and puffed material and raw material, standing, cooling and molding to obtain Cordyceps militaris culture medium.
Diluting 10 times of Cordyceps militaris strain with 10g/L sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, uniformly spraying the Cordyceps militaris liquid strain onto the surface of the prepared Cordyceps militaris culture medium, and inoculating 8mL of Cordyceps militaris culture medium per bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 4 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 20 ℃ under illumination of 800Lux. Harvesting on 53 th day after inoculation, weighing the harvested fresh cordyceps militaris fruiting bodies, and calculating the biotransformation rate of cordyceps militaris according to the formula (1), wherein the biotransformation rate is 172.5%.
Comparative example 2
The cordyceps militaris culture medium of the comparative example consists of single extruded and puffed materials, the raw material proportion is adjusted, the steps of raw material preparation and mixing are reduced, and other steps and parameters are consistent with those of example 3. The specific operation is as follows:
selecting naked oats, rice and soybeans as raw materials, sieving to remove impurities, and performing separation according to the weight ratio of the naked oats: rice: soybean =80:15:5, grinding, sieving with 80 mesh sieve, adjusting water content to 18%, feeding with a DL 70-II twin-screw extruder at 20rpm and 16 rpm0rpm, the extrusion temperature is 165 ℃, extruded and expanded material particles with the particle size of-5 meshes to +7 meshes are obtained, and the bulk density of the extruded and expanded material is 0.42g/cm 3 . Bottling the prepared extruded and puffed material, performing moist heat sterilization at 121 deg.C for 30min, filling 65 deg.C sterile water with addition amount of 3.5 times of total mass of extruded and puffed material and raw material, standing, and cooling for molding to obtain Cordyceps militaris culture medium.
Diluting 10 times of Cordyceps militaris strain with 10g/L sterile water under aseptic condition to obtain Cordyceps militaris liquid strain, uniformly spraying the Cordyceps militaris liquid strain onto the surface of the prepared Cordyceps militaris culture medium, and inoculating 8mL of Cordyceps militaris culture medium per bottle. Placing the inoculated cordyceps militaris culture medium into a culture room for dark culture, and culturing for 4 days at 18 ℃ until the surface of the cordyceps militaris culture medium is completely covered by cordyceps militaris mycelia; after completion of the dark culture, cultivation management was performed at 20 ℃ under illumination of 800Lux. Harvesting on 50 th day after inoculation, weighing the harvested fresh cordyceps militaris fruiting bodies, and calculating the biotransformation rate of cordyceps militaris according to the formula (1), wherein the biotransformation rate is 163.9%.
In conclusion, it can be seen that, when the cordyceps militaris is cultivated by the culture medium prepared by the single extrusion swelling material, the late growth of cordyceps militaris hyphae is insufficient, and the biotransformation rate of cordyceps militaris is obviously lower than that of the culture medium prepared by mixing the extrusion swelling material and the raw material.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A preparation method of a cordyceps militaris culture medium based on an extrusion puffing technology is characterized by comprising the following steps:
(1) Crushing the grain raw material, adjusting the water content, extruding and puffing to obtain an extruded and puffed material;
(2) Pulverizing bean raw materials to obtain raw material;
(3) And mixing the extruded and expanded material with the raw material, sterilizing, and adding water to obtain the cordyceps militaris culture medium.
2. The method of claim 1, wherein the cereal material comprises one or more of wheat kernel, naked oat, barley, rice, corn and silkworm chrysalis meal; the bean material comprises one or more of semen glycines, semen Sojae Atricolor, semen Viciae Fabae, semen lablab album, semen Phaseoli Radiati and semen Phaseoli.
3. The method according to claim 2, wherein the pulverized particle size in the step (1) is 60 to 100 mesh, and the water content is 18 to 25%.
4. The method of claim 3, wherein the temperature of the extrusion is 150 to 200 ℃.
5. The production method according to claim 4, wherein the extruded dilatant is a pellet of-3 mesh to +7 mesh; the bulk density of the extruded and expanded material is 0.28-0.45 g/cm 3 。
6. The method according to claim 5, wherein the raw material is a pellet of-10 to +30 mesh.
7. The method of claim 6, wherein the mass ratio of the extruded puffed material to the raw meal is 6:0.5 to 1.
8. The method according to claim 7, wherein the temperature of the water is 80 to 95 ℃; the addition amount of the water is 2-4 times of the total mass of the extruded and expanded material and the raw material.
9. A culture medium of Cordyceps militaris prepared by the method of any one of claims 1 to 8.
10. An application of the cordyceps militaris culture medium of claim 9 in improving the yield and the biotransformation rate of cordyceps militaris fruiting bodies.
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| CN104823692A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Ophiocordyceps sinensis wheat |
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| CN112273142A (en) * | 2020-11-12 | 2021-01-29 | 江苏康能生物工程股份有限公司 | Culture method for increasing content of crude polysaccharide in cordyceps militaris sporocarp |
| CN115152527A (en) * | 2022-06-22 | 2022-10-11 | 辽宁省微生物科学研究院 | Method for cultivating high-quality cordyceps militaris sporocarp in short period |
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|---|---|---|---|---|
| CN104823692A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Ophiocordyceps sinensis wheat |
| CN104823693A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis soybeans |
| CN108117416A (en) * | 2016-11-26 | 2018-06-05 | 辽宁弘侨生物科技股份有限公司 | A kind of cordyceps culturing medium and preparation method thereof |
| CN112273142A (en) * | 2020-11-12 | 2021-01-29 | 江苏康能生物工程股份有限公司 | Culture method for increasing content of crude polysaccharide in cordyceps militaris sporocarp |
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