CN103271281A - Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation - Google Patents
Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation Download PDFInfo
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- CN103271281A CN103271281A CN2013102295811A CN201310229581A CN103271281A CN 103271281 A CN103271281 A CN 103271281A CN 2013102295811 A CN2013102295811 A CN 2013102295811A CN 201310229581 A CN201310229581 A CN 201310229581A CN 103271281 A CN103271281 A CN 103271281A
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Abstract
The invention discloses a method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation. According to the method, people can safely and conveniently eat and digest nutritional ingredients of cordyceps militaris, and simultaneously, problems of complicated production processes and high costs of cordyceps militaris products are solved. The method adopts a solid-state fermentation manner, and a fermentation substrate is strictly sterilized, so that in the process of fermentation, the microbial interference can be basically eliminated, the solid-state fermentation is easier to master when compared with liquid-state fermentation, and design requirements and costs of a biochemical reactor are low. The whole production process comprises three links as follows: manufacturing a liquid cordyceps militaris culture, manufacturing a solid-state fermentation medium of the coarse cereals, and performing inoculation and fermentation; and the three links can realize automatic operation, so that the human resource cost can be reduced.
Description
Technical field
The present invention relates to a kind of method of producing the fermentation coarse cereals, specifically a kind of method of utilizing solid state fermentation to produce Cordyceps militaris fermentation coarse cereals belongs to the food engineering field.
Background technology
Cordyceps militaris is Ascomycotina, the ergot Zoopagales, and the type sepecies of Clavicipitaceae, Cordyceps are a kind of medicinal fungis of preciousness, for a long time, the treasure that is considered as promoting longevity by people always.Modern medicine proves that Cordyceps militaris has the artery sclerosis of preventing, improves immunity, antitumor, the anti-ageing multiple effect of waiting for a long time.The primary raw material that is used for developing the Cordyceps militaris product at present is tame fructification, but relatively poor because of the fruiting bodies of cordyceps militaris mouthfeel, can not directly eat, and fruiting bodies of cordyceps militaris belongs to medicine, can not in food, arbitrarily add, thereby make the consumption of Cordyceps militaris product have certain limitation.And the fruiting bodies of cordyceps militaris formation cycle is long, and required labour intensity is big, has improved the cost of Cordyceps militaris product.The existence of these problems has restricted the development of Cordyceps militaris product and has popularized.How can produce and be easy to Cordyceps militaris product edible and that digest and assimilate, simplify production technology simultaneously, reduce cost, be the common technical tasks of paying close attention to of present people.Still there is not at present a kind of record that utilizes solid state fermentation to produce the method for Cordyceps militaris fermentation coarse cereals.
Summary of the invention
The objective of the invention is the problem at prior art and product existence, a kind of method of utilizing solid state fermentation to produce Cordyceps militaris fermentation coarse cereals is provided, make people can be safely, edible and digest and assimilate the nutritional labeling of Cordyceps militaris easily, solve Cordyceps militaris product processes complexity, problem that cost is high simultaneously.
The objective of the invention is to be achieved through the following technical solutions, it is characterized in that: be main fermentation substrate with coarse cereals, the mode by solid state fermentation is grown the Cordyceps militaris mycelia in coarse cereals, obtain being rich in the mycelial coarse cereals of Cordyceps militaris, comprises the steps:
1, with after the activated cultivation of slant preservation bacterial classification, inserts in the semi-synthetic fluid nutrient medium, cultivate 8-10d, namely get the Cordyceps militaris liquid spawn for 20-28 ℃;
2, the round of packing into after coarse cereals and water are mixed according to the weight ratio of 4:1-6:1, the 15-40min that sterilizes under the 115-132 ℃ of condition, sterilization is cooled to normal temperature after finishing under cleaning condition, standby;
3, the inoculum concentration according to 10-15% inserts the Cordyceps militaris liquid spawn in coarse cereals, under 20-28 ℃ of condition, after lucifuge is cultivated 8-10d, change over to again under the illumination condition of 100lx-150lx and cultivate 15-20d, finally air dry at ambient temperature namely gets Cordyceps militaris fermentation coarse cereals.
In the product of the present invention, coarse cereals refer to one or more mixing in Chinese sorghum, millet, buckwheat, oat, barley, broom corn millet, broomcorn millet, heart of a lotus seed benevolence, Kidney bean, mung bean, red bean, broad bean, pea, cowpea, lens, the black soya bean.
After the activated cultivation of slant preservation bacterial classification, insert in the semi-synthetic fluid nutrient medium, the primary raw material of semi-synthetic fluid nutrient medium has potato or wheat bran liquor, glucose, skimmed milk power, cultivate 8-10d for 20-28 ℃, make the Cordyceps militaris liquid spawn, wherein the primary raw material of semi-synthetic fluid nutrient medium composition is expressed as potato or wheat bran liquor: glucose: skimmed milk power=1000:20:2 by weight, wherein optimum culturing temperature is 25 ℃, and best incubation time is 8d.
The round of packing into after coarse cereals and water mixed according to the weight ratio of 4:1-6:1, behind autoclave sterilization, cooling obtains coarse cereals solid state fermentation substrate under cleaning condition.Wherein the optimum proportioning of coarse cereals and water is 5:1, and best sterilising conditions is 121 ℃, 35min, and round can be used fermentation tank (pond, cylinder, bag, bottle etc.).
Further, optimum temperature range is 23-25 ℃ in the step (3), and the Best Times that lucifuge is cultivated is 8d, and best incubation time is 15d under the illumination condition, and best intensity of illumination is 110lx.
The present invention and prior art have many advantages and good effect:
1, pure coarse cereals ferment, and are rich in nutrition and are easy to eat and digest and assimilate;
Cordyceps militaris fermentation coarse cereals are to be that raw material carries out solid state fermentation and forms with coarse cereals, can cater to compatriots to the preference of coarse cereals, by fermentation, the drug effect of Cordyceps militaris and the nutrition of coarse cereals can combine, the Cordyceps militaris fermentation coarse cereals that finally obtain contain multiple nutritional components and mineral matter element, particularly be rich in cordycepin and cordycepic acid, cordycepin content 〉=600mg/100g wherein, cordycepic acid content 〉=3g/100g, has the raising immunity of organisms, improve microcirculqtory system, effect such as antitumor, because Cordyceps militaris fermentation coarse cereals are to exist with solid-state form, so people can be as eating it with eating common coarse cereals convenient;
2, production link has good security through strict monitoring;
The Cordyceps militaris liquid spawn produces and carries out in strict accordance with the sterile working rules, being used for the liquid medium starting material of bacterial classification production and kind and the consumption of additive is to need strict control, its quality will detect through strict, strictness monitoring by to each production link makes Cordyceps militaris mycelia coarse cereals possess good security;
3, production technology is simple, easily grasps, and cost is low;
The present invention adopts the form of solid state fermentation, and used fermentation substrate is sterilized through strictness, therefore, substantially can get rid of the interference of assorted bacterium during the fermentation, than the easier grasp of liquid state fermentation, and biochemical reactor designing requirement and expense are also low, and the whole production flow process is divided into three links: make the Cordyceps militaris liquid spawn; Make the coarse cereals solid-state fermentation culture medium; Inoculation and fermentation; Three links can realize automated job, can reduce cost of human resources.
The specific embodiment
The invention will be further described with embodiment below, but do not limit the present invention in any way.
The present invention is a kind of method of utilizing solid state fermentation to produce Cordyceps militaris fermentation coarse cereals, comprises the steps:
1, with in the slant preservation bacterium on the PDA flat board activation culture 8d, the bacterium piece of beating cut-off footpath 0.5cm insert the 500ml triangle that contains 150ml potato glucose fluid nutrient medium bottled in, 25 ℃, 150r/min concussion are cultivated 8d, namely get the Cordyceps militaris liquid spawn;
2, get 50g coarse cereals and 10ml water and pack in the polypropylene plastics pocket of 20*22cm 121 ℃ of sterilization 35min into.Sterilization is cooled to normal temperature after finishing under cleaning condition, standby;
3, every bag of coarse cereals insert 8ml Cordyceps militaris liquid spawn, under 23-25 ℃ of condition, after lucifuge is cultivated 8d, continue to cultivate 15d under the 110lx intensity of illumination, take off bag, and air dry at ambient temperature namely obtains Cordyceps militaris fermentation coarse cereals;
Claims (2)
1. method of utilizing solid state fermentation to produce Cordyceps militaris fermentation coarse cereals, it is characterized in that: be main fermentation substrate with coarse cereals, mode by solid state fermentation is grown the Cordyceps militaris mycelia in coarse cereals, obtain being rich in the mycelial coarse cereals of Cordyceps militaris, comprises the steps:
(1), with after the activated cultivation of slant preservation bacterial classification, insert in the semi-synthetic fluid nutrient medium, cultivate 8-10d, namely get the Cordyceps militaris liquid spawn for 20-28 ℃;
(2), the round of packing into after coarse cereals and water mixed according to the weight ratio of 4:1-6:1, the 15-40min that sterilizes under the 115-132 ℃ of condition, sterilization is cooled to normal temperature after finishing under cleaning condition, standby;
(3), the inoculum concentration according to 10-15% inserts the Cordyceps militaris liquid spawn in coarse cereals, under 20-28 ℃ of condition, after lucifuge is cultivated 8-10d, change over to again under the illumination condition of 100lx-150lx and cultivate 15-20d, finally air dry at ambient temperature namely gets Cordyceps militaris fermentation coarse cereals;
The primary raw material of described semi-synthetic fluid nutrient medium has potato or wheat bran liquor, glucose, skimmed milk power, wherein the primary raw material of semi-synthetic fluid nutrient medium composition is expressed as potato or wheat bran liquor: glucose: skimmed milk power=1000:20:2 by weight;
Described coarse cereals are one or more mixing in Chinese sorghum, millet, buckwheat, oat, barley, broom corn millet, broomcorn millet, heart of a lotus seed benevolence, Kidney bean, mung bean, red bean, broad bean, pea, cowpea, lens, the black soya bean.
2. method according to claim 1, it is characterized in that: wherein the proportioning of coarse cereals and water is 5:1, and sterilising conditions is 121 ℃, 35min, and round is fermentation tank.
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| CN104115672A (en) * | 2014-07-24 | 2014-10-29 | 巩玉升 | Cultivation method for tomato cordyceps militaris fruits |
| CN104207121A (en) * | 2014-07-07 | 2014-12-17 | 郑州乐嫒商贸有限公司 | Immunity-enhancing, blood-glucose-reducing, blood-pressure-reducing and blood-fat-reducing diet-therapy health-caring product |
| CN104686937A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Worm grass sago |
| CN104686200A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis gorgon fruits |
| CN104686205A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis oats |
| CN104686201A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis teff |
| CN104686199A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis highland barley |
| CN104687117A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps Chinese chestnut |
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| CN104686202A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis sweet potatoes |
| CN104718987A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis chenopodium quinoa |
| CN104718989A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis dasheen |
| CN104719782A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis mung beans |
| CN104757428A (en) * | 2015-03-20 | 2015-07-08 | 江南大学 | Chinese caterpillar fungus barley |
| CN104757454A (en) * | 2015-03-20 | 2015-07-08 | 江南大学 | Chinese caterpillar fungus lotus root |
| CN104823695A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Aweto peanut |
| CN104824586A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis cassava |
| CN104824588A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis potatoes |
| CN104824728A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis sesames |
| CN104824574A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis broad bean |
| CN104823692A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Ophiocordyceps sinensis wheat |
| CN104823690A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis and cordyceps millitaris buckwheat |
| CN104823697A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps peas |
| CN104823689A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps millet |
| CN104855135A (en) * | 2015-03-20 | 2015-08-26 | 江南大学 | Cordyceps sinensis broomcorn product |
| CN105124398A (en) * | 2015-07-15 | 2015-12-09 | 湖北省农业科学院农产品加工与核农技术研究所 | Production method of functional frozen dough |
| CN106165824A (en) * | 2016-07-12 | 2016-11-30 | 江苏大学 | A kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food |
| CN107373382A (en) * | 2017-08-24 | 2017-11-24 | 河南农业大学 | A kind of preparation method of Cordyceps militaris fermented soya bean |
| CN108684824A (en) * | 2018-06-12 | 2018-10-23 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean soya-bean milk and preparation method thereof |
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| CN104207121B (en) * | 2014-07-07 | 2016-01-06 | 郑州乐嫒商贸有限公司 | A kind of immunity that improves reduces by three high food therapeutic healths |
| CN104115672A (en) * | 2014-07-24 | 2014-10-29 | 巩玉升 | Cultivation method for tomato cordyceps militaris fruits |
| CN104757454A (en) * | 2015-03-20 | 2015-07-08 | 江南大学 | Chinese caterpillar fungus lotus root |
| CN104824586A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis cassava |
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| CN104687117A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps Chinese chestnut |
| CN104686204A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis naked barley |
| CN104686202A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis sweet potatoes |
| CN104718987A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis chenopodium quinoa |
| CN104718989A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis dasheen |
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| CN104757428A (en) * | 2015-03-20 | 2015-07-08 | 江南大学 | Chinese caterpillar fungus barley |
| CN104686200A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis gorgon fruits |
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| CN104824574A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis broad bean |
| CN104823692A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Ophiocordyceps sinensis wheat |
| CN104823690A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis and cordyceps millitaris buckwheat |
| CN104823697A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps peas |
| CN104823689A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps millet |
| CN104855135A (en) * | 2015-03-20 | 2015-08-26 | 江南大学 | Cordyceps sinensis broomcorn product |
| CN104686937A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Worm grass sago |
| CN105124398A (en) * | 2015-07-15 | 2015-12-09 | 湖北省农业科学院农产品加工与核农技术研究所 | Production method of functional frozen dough |
| CN106165824A (en) * | 2016-07-12 | 2016-11-30 | 江苏大学 | A kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food |
| CN107373382A (en) * | 2017-08-24 | 2017-11-24 | 河南农业大学 | A kind of preparation method of Cordyceps militaris fermented soya bean |
| CN108684824A (en) * | 2018-06-12 | 2018-10-23 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean soya-bean milk and preparation method thereof |
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Application publication date: 20130904 |