CN106165824A - A kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food - Google Patents
A kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food Download PDFInfo
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- CN106165824A CN106165824A CN201610543868.5A CN201610543868A CN106165824A CN 106165824 A CN106165824 A CN 106165824A CN 201610543868 A CN201610543868 A CN 201610543868A CN 106165824 A CN106165824 A CN 106165824A
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- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011552 rat model Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229940065287 selenium compound Drugs 0.000 description 1
- 150000003343 selenium compounds Chemical class 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000003805 vibration mixing Methods 0.000 description 1
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- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food, relate to technical field of bioengineering.Described preparation method sequentially passes through test tube amplification culture, liquid submerged culture and seed tank amplification culture, solid fermentation is cultivated, dry and pulverising step prepares and has regulation blood fat, blood glucose and blood pressure, radioprotective and the functional food of suppression tumor.The present invention, with Semen Fagopyri Esculenti and rice, Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers. etc. as solid matrix, with Cordyceps for strain by solid fermentation, converts buckwheat flavonoids, simultaneously synthesizing cordycepin and Cordyceps polysaccharide；Obtained product, flavones content 2～50mg/g butt matter, Cordyceps polysaccharide content 10～100mg/g butt matter, cordycepin content 1～20mg/g butt matter, can be used for extracting flavone, Cordyceps polysaccharide and cordycepin, produce treatment and reduce blood fat, blood glucose and blood pressure, radioprotective and the tablet of suppression tumor or capsule.
Description
Technical field
The present invention relates to technical field of bioengineering, be specifically related to one and utilize Cordyceps to convert the Semen Fagopyri Esculenti functional food of production
The method of product.
Background technology
Semen Fagopyri Esculenti is the dicotyledonous annual crop of buckwheat, belongs to Minor Crops in China, plants with a long history.Buckwheat
Wheat is short-day plant, and happiness cools moistening, non-refractory, fears frost, and drought resisting is resistance to lean, and production period is short, whole period of duration only 2-3
Individual month.Semen Fagopyri Esculenti, as grain, feedstuff dual-purpose type crop, becomes the mountain area people already and tides over a lean year the main food combated a natural disaster.The battalion of Semen Fagopyri Esculenti
Support and enrich and comprehensive, have the laudatory title of " kings of grain rice ".Comparing with other large cereal crops, Semen Fagopyri Esculenti has a lot of uniqueness
Advantage: the content of protein, fat, vitamin and dietary fiber contained in Semen Fagopyri Esculenti is all generally higher than Semen Tritici aestivi, rice and Semen Maydis,
And the chlorophyll not contained containing other cereal crops and rutin.Semen Fagopyri Esculenti has a lot of health care, can prevent and treat hypertension, sugar
Urine disease, and can be anticancer, anti-aging.Modern medicine study proves that Semen Fagopyri Esculenti has a following function: (1) blood sugar lowering, preventing and treating glycosuria
Sick: the starch in Semen Fagopyri Esculenti mostly is resistant starch, blood glucose after meal can be suppressed to raise, the secretion of regulation insulin；Rich in reed in Semen Fagopyri Esculenti
The bioflavonoids such as fourth, can promote the recovery of beta Cell of islet, thus reduce blood glucose and improve carbohydrate tolerance and to antiadrenergic drug
Blood glucose increasing effect, therefore can effectively prevent and treat diabetes；(2) blood fat reducing, prevention cardiovascular disease: the rutin energy in Semen Fagopyri Esculenti
Strengthening blood vessel, increase capillary permeability, play reduction cholesterol and the effect of blood fat, and can prevent cardiovascular and cerebrovascular disease
And hypertension；(3) free radical, anticancer is removed: it is theoretical that the composition of Radix Et Rhizoma Fagopyri Tatarici flavonoid molecular structure meets the light base of effective phenol, has very
Strong Scavenging ability.Rich in selenium element in Semen Fagopyri Esculenti, selenium compound can capture human senility free radical, controls cell and divides
Split breeding, repair DNA (deoxyribonucleic acid), play antitumaous effect；(4) anti-constipation, radioprotective: containing resistant starch in Semen Fagopyri Esculenti, can promote
Defecation increases, and has certain curative effect to preventing cecitis and constipation and other diseases.Semen Fagopyri Esculenti contains more cystine, has well
Radioactivity defencive function；(5) Leukemia Cell Proliferation is hindered: containing 8 hatching egg enzyme retardants in Semen Fagopyri Esculenti, they are proteolytic enzyme
Hinder material one, the propagation of leukaemia can be hindered.
Inventor, through further investigation and industrialized production test repeatedly, finds out industrially scalable conversion Semen Fagopyri Esculenti raw
Produce functional food technology, the present invention no matter from the mode of production and the product of production of product, be all different from conventional patent and
The content of article report, a kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food.
Summary of the invention
, problem to be solved
For the above-mentioned problems in the prior art, the present invention provides one to utilize Cordyceps to convert Semen Fagopyri Esculenti and produces functional food
The method of product, it is with the Semen Fagopyri Esculenti pulverized and rice, Semen Tritici aestivi, Semen Maydis and Sorghum vulgare Pers. as solid matrix, with Cordyceps for strain by solid
Body ferments, and converts Semen Fagopyri Esculenti, makes Cordyceps mycelium have the function of pharmacy function with Semen Fagopyri Esculenti interaction production by fermentation
Property food, technique is simple, and in obtained product, flavones content 2～50mg/g butt matter, Cordyceps polysaccharide content 10～
100mg/g butt matter, cordycepin content 1～20mg/g butt matter.This product can also extract cordycepin and flavone, many further
The thus obtained product of sugar has the pharmacognosy function identical with fermented product.
, technical scheme
In order to solve the problems referred to above, the technical solution adopted in the present invention is as follows:
A kind of described method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food, described preparation method is with the buckwheat pulverized
Wheat, rice, Semen Tritici aestivi, Semen Maydis and Sorghum vulgare Pers. are main matrix, with Cordyceps as starting strain, sequentially pass through test tube amplification culture, liquid
Body shake-flask culture and the step such as seed tank amplification culture, solid fermentation cultivation；Described functional food can be again respectively through carrying
Taking prepared flavone and cordycepin extractive, Cordyceps polysaccharide extract, wherein in flavone extract, flavones content is 25～60%, worm
In grass element yeast powder, cordycepin content is 30～70%, Cordyceps polysaccharide purity 20～60%.
Described a kind of Cordyceps convert Semen Fagopyri Esculenti produce functional food method, the buckwheat that the method is used
Including general buck wheat (Fagopyrum esculentum), Radix Et Rhizoma Fagopyri Tatarici (Fagopyrum tartaricumGaertn) or gold buckwheat
Wheat (Fagopyrum cymosumL.)；The Cordyceps strain used include Cordyceps (Cordyceps militaris) or
Cordyceps fungus (Cordyceps sinensis)。。
A kind of Cordyceps converts the method that Semen Fagopyri Esculenti produces functional food, carries out as steps described below:
A1 test tube amplification culture: Cordyceps slant strains be inoculated in potato dextrose medium and cultivate, prepares worm
Grass bacterium test tube slant strain；
A2 liquid submerged culture: the Cordyceps test tube slant strain obtained by step A1 is inoculated into equipped with liquid submerged culture base
Shaking flask in, cultivate, prepare Cordyceps liquid shaking bottle strain；
A3 seed tank amplification culture: be inoculated in seed tank culture base by the Cordyceps liquid shaking bottle strain obtained by step A2
Row is cultivated, and makes Cordyceps seed tank strain；
A4 solid fermentation is cultivated: is inoculated in solid medium by the Cordyceps seed tank strain obtained by step A3, and mixes
Uniformly, carry out fermentation culture, prepare Cordyceps solid fermentation thing；
A5 packs: Cordyceps solid fermentation thing converts the functional food of Semen Fagopyri Esculenti through drying, pulverize, pack prepared Cordyceps.
Preferably, a kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti, carry out as steps described below:
B1 test tube amplification culture: slant strains in Cordyceps test tube is cut into 3 × 3 mm fritter strains, inoculates a fritter to test tube
In slant medium, cultivating 4～15 days for 20～35 DEG C, prepare test tube slant strain, 4 DEG C, this test tube slant saves backup；
B2 liquid submerged culture: the test tube slant strain obtained by step B1 is cut into 3 × 3 mm fritter strains, picking 3～10
Block is inoculated in the 250mL triangular flask equipped with 20～150mL liquid submerged culture bases, triangular flask rotating speed be 50～200 turns/
Point, under conditions of temperature 20～35 DEG C, cultivate 18-86h, make liquid shaking bottle strain；
B3 seed tank amplification culture: the liquid shaking bottle strain obtained by step B2 is inoculated in seed tank amplification culture base, and
Described liquid shaking bottle strain and seed tank amplification culture base are the inoculum concentration inoculation of 1～20% by volume, are 20～35 in temperature
DEG C, speed of agitator is 50～160 revs/min, is 0.2 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
～under the conditions of the ventilation of 1.8:1, cultivate 18～96h, make Cordyceps seed tank strain；
B4 solid fermentation is cultivated: accessed in solid fermentation culture medium by the Cordyceps seed tank strain obtained by step B3, and institute
State the inoculum concentration inoculation that Cordyceps seed tank strain and solid fermentation culture medium are 2～10% by weight, in temperature 20～35
DEG C, humidity 80% is fermented 5～20 days, and wherein every 3～6 days, stirring once, prepares solid fermentation thing；
B5 solid fermentation thing, after 80～120 DEG C dry 10～24 hours, is crushed to below 100 mesh, is packaged to be and can eat
Functional food；
Preferably, liquid submerged culture base described in step B2 is that sterilizing 10～60 min prepares under the conditions of 100～130 DEG C,
And containing wheat bran 5～20g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 5～20g.
Preferably, the base of seed tank amplification culture described in step B3 is sterilizing 10～60 min system under the conditions of 100～130 DEG C
, and containing wheat bran 5～20g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 5～20g.
Preferably, solid fermentation culture medium described in step B4 is sterilizing 90～360 min under the conditions of 100～130 DEG C
Prepare, and each kilogram pulverize Semen Fagopyri Esculenti add 200～400g rice, 100～300g Semen Tritici aestivi, 100～300g Semen Maydis
With solid matrix raw materials such as 700～300g Sorghum vulgare Pers..
Preferably, the raw material in described solid fermentation culture medium mixes with water by weight the ratio for 1:0.6～1.5.
In step A4, A5 or step B4, B5, functional food carries out the extracting method of flavone and comprises the steps:
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
60～the ethanol of 100% of 5～20 times of volumes of filament weight, repeatedly extracts 3～5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3～18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone functional
Food, this food flavones content is 25～60%.
In step A4, A5 or step B4, B5, functional food carries out the extraction of Cordyceps polysaccharide and comprises the steps:
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds 5～20 times of volumes of mycelium weight
Distilled water, 80～100 DEG C are repeatedly extracted 3～5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, add residual volume 0.5～
95% ethanol of 1.5 times, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extraction raffinate volume 2.5～
95% ethanol of 3.5 times, crystallizes 5～18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide
Purity 20～60%.
In step A4, A5 or step B4, B5, functional food carries out the extraction of cordycepin and comprises the steps:
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and the volume ratio of the 10-40 times of volume adding functional food weight is dense
Degree is 60-100% ethanol, and 60-90 DEG C is incubated 4-6 hour；Filtering, residue adds the volume of 10-40 times of volume of residue weight
Specific concentration is 60-100% ethanol, and 60-90 DEG C is incubated 4-6 hour, repeats 2-3 time；
E2 supernatant macroporous resin static adsorption 5-20 hour of 1/50-1/5 volume, 10-20 times of resin volume after absorption
Water washs, and installs in chromatographic column, and with the 20-80% ethanol desorbing of 10-20 times of resin volume, stripping liquid is incorporated in temperature 20-50
DEG C, it is concentrated in vacuo to the 1/100-1/50 of supernatant volume；
Preferably macroporous resin includes all low pole macroporous resins, such as AB-8 type, D-113 type, D-152 type, HD-2 type, HZD-
3 types, HZD-5 type etc.；
E3 concentrated solution is by regulation flow speed control air inlet temperature 160-190 DEG C, and air outlet temperature 100-120 DEG C carries out spray dried
Dry obtain cordycepin yeast powder.Cordycepin purity 30～70% in cordycepin yeast powder.
, beneficial effect
Compared to prior art, the invention have the benefit that
(1) present invention is on the basis of drawing herb fermenting pharmaceutical technology, in conjunction with the advantage of modern solid-state fermentation, uses solid-state to send out
Ferment converts Semen Fagopyri Esculenti, and during overcoming liquid fermentation, the suppression of Cordyceps mycelial growth is made by substrate (active component of Semen Fagopyri Esculenti)
With, simultaneously without liquid fermentation waste water and residue contamination；
(2) a kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food of the present invention, is not simple utilization
Then the active component of active component and Cordyceps that process for separation and purification extracts Semen Fagopyri Esculenti carries out compounding forming, but with Cordyceps
For strain by solid fermentation, directly eat after converting Semen Fagopyri Esculenti, both improve effect of Semen Fagopyri Esculenti active component, enhanced again Cordyceps
Bacterium active component Cordyceps polysaccharide and the amount of cordycepin, can realize industrialization large-scale production；
(3) a kind of Cordyceps of the present invention convert Semen Fagopyri Esculenti produce functional food method, in its product flavones content 2～
50mg/g butt matter, Cordyceps polysaccharide content 10～100mg/g butt matter, cordycepin content 1～20mg/g butt matter.There is regulation
The effects such as blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.The cordycepin extracted from this product and flavone, Cordyceps polysaccharide
There is regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor function equally.
Accompanying drawing explanation
Fig. 1 is the preparation flow of a kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food of the present invention
Schematic diagram.
Detailed description of the invention
The mensuration of described flavones content employing sodium nitrite-aluminum nitrate assay method (Deng Bin, Jiang Gangbiao, old
Six is flat. the content [J] of Food wrapping paper Rhizoma Dioscoreae esculentae total flavones. and Packaging Engineering, 2008,29 (1): 27 ~
29).
The mensuration of described cordycepin content uses high performance liquid chromatography (Xia Min: rp-hplc determination
The content of cordycepin in Cordyceps militaris (L.) Link.. Huaiyingong College journal, 2004,13 (3) 22～24).
Described Cordyceps polysaccharide content deducts reducing sugar equal to total sugar, and the mensuration of total sugar uses sulfuric acid-phynol method (Zhaoyang
Nanmu, Chang Jidong. Phenol sulfuric acid procedure and indirect iodometric processes measure ganoderma polyoses content and compare [J]. edible fungi, 2007, (3): 58
～61.), content of reducing sugar measures employing 3,5-dinitrosalicylic acid system (Miller GL. Use of
dinitrosalicylic acid reagent for determination of reducing sugars.
Analytical Chemistry. 1959,1:426 ~ 428.).
Described Cordyceps converts Semen Fagopyri Esculenti and produces the evaluation methodology employing high blood lipid model Mus of functional food regulation blood fat
Evaluate (Cao Min etc.: sanchi flower total saponine hypotensive effect is studied. the bright traditional Chinese medical science, and 2012,27 (7): 1314-1315).Concrete operations
For: after 40 mices adapt to feed 5d with normal feedstuff, take tail hematometry Triglycerides in Serum content, with triglyceride
Level is randomly divided into 4 groups: Normal group, hyperlipidemia matched group, low dosage experimental group, high dose experimental group.Normal control
Group mice feed normal feedstuff (12% casein, 60.98% corn starch, 15% sucrose, 7% Semen Maydis oil, 1% vitamin Icing Sugar, 4%
Mineral salt, 0.02% cod-liver oil)；(2% gallbladder is solid for 15.75% lard stearin, 7.79% sucrose for hyperlipidemia model control group fed high lipid food
Alcohol, 0.5% cholic acid, 73.96% normal feedstuff；Low dosage and high dose group mice feed per kilogram high lipid food and contain 10 grams and 20
Gram Cordyceps convert Semen Fagopyri Esculenti functional food.Each group mice the most freely ingests and drinks water, animal housing's temperature 18～22 DEG C.Continuously
After being administered surrounding, tail vein takes blood, analyzes serum triglyceride, T-CHOL, high density lipoprotein and low density lipoprotein
Protein content
Described Cordyceps converts Semen Fagopyri Esculenti and produces the evaluation methodology employing barbital of functional food regulation blood fat and ligature left kidney
(yellow will is new: Herba Visci, the hypotensive activity of the Cortex Eucommiae and acute toxicity in the Hypertensive Rats evaluation of tremulous pulse combined induction
Experimentation. research and development of natural products, 2003,15 (3): 245-248).Hypertension model Mus is divided into three groups of blanks
The Cordyceps that group, low dose group and high dose group feed normal feedstuff respectively, per kilogram contains 10 grams converts the functional food of Rhizoma Dioscoreae
The Cordyceps that the normal feedstuff of product and per kilogram contain 20 grams converts the normal feedstuff of Rhizoma Dioscoreae functional food.Successive administration 14 days
Blood pressure after measuring before being administered and being administered.
Described Cordyceps converts Semen Fagopyri Esculenti and produces the evaluation methodology employing alloxan induction of functional food regulation blood glucose
Diabetic rat model evaluation.Induction reference literature (the Effect of FeSO such as Zhicai Zhang of model mouse4
treatment on glucose metabolism in diabetic rats. Biometals (2008) 21:685–
691) carry out.Hyperglycemia model Mus is divided into three groups of blank groups, low dose group and high dose group to feed normal feedstuff, every respectively
Kilogram convert the normal feedstuff of Rhizoma Dioscoreae functional food containing the Cordyceps of 10 grams and Cordyceps that per kilogram contains 20 grams converts
The normal feedstuff of Rhizoma Dioscoreae functional food.Successive administration measures the blood glucose value before being administered and after administration for 28 days.
Described Cordyceps converts the Semen Fagopyri Esculenti production radiation-resistant evaluation methodology of functional food and uses radiation irradiation in rats to enter
Row evaluation (a diplodocus man of virtue and ability. the effect study of Lac regis apis Antiradiation injury. Heilungkiang medical science, 2012,36 (10): 724-726).30
SD male rat feeds normal feedstuff respectively, converts the normal feedstuff 15 days of Semen Fagopyri Esculenti functional food containing 1% and 2% Cordyceps
After, rat is feeding60Co gamma-rays carries out total irradiation, away from radiation source 1.0 m close rate 2.0 Gy, accumulated dose 200rad.Irradiate
Measuring body weight, mouse mortality rate after 10 days, tail vein takes haemanalysis quantity of leucocyte.
Described Cordyceps converts Semen Fagopyri Esculenti and produces the evaluation methodology employing suppression Primary Hepatic of functional food suppression tumor
Cancerous cell Hepg2 growth effect (Zhouning County, Chen Jiangtao, Yu Wenyan. Water Extract of Ficus Carica to suppression tumor cell proliferation
The preliminary study of effect. Xinjiang Medicine University's journal. 2016,39 (1): 42-47).Hepg2 groups of cells complete culture solution
Adjusting cell concentration is 2 × 104Individual/mL, is inoculated in 96 orifice plates, and every hole cumulative volume is 200 μ L, and overnight incubation makes cell attachment,
Next day respectively with the Cordyceps of various dose convert the ethanol extract of Semen Fagopyri Esculenti functional food and aqueous extract (substrate: water (or
Dehydrated alcohol)=1:10,70 DEG C are extracted 4 hours) and processing cell 24h respectively, matched group adds PBS and processes 24h, and each mensuration connects
Plant 3 multiple holes, be placed in 37 DEG C, 5%CO2After incubator processes 24, every hole adds 5mg/mL tetrazolium bromide (MTT) reagent 20 μ L, continues training
Support 4h, then add dimethyl sulfoxide (DMSO) 150 μ L, vibration mixing 10min, with microplate reader (λ=490nm) measure OD value (OD value and
Viable count is directly proportional), cell inhibitory rate=1-(dosing group OD value/matched group OD value), experiment is repeated 3 times.
Below in conjunction with specific embodiment, the present invention is further described below.
Embodiment 1
Utilize Cordyceps to convert Semen Fagopyri Esculenti to produce the method for functional food and specifically include following steps as it is shown in figure 1, a kind of:
(1) test tube amplification culture: by Cordyceps (Cordyceps militaris) CICC 14015 test tube slant strain is cut into 3
× 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 15 days for 20 DEG C, prepare test tube slant strain, should
4 DEG C, test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 10 pieces
Being inoculated in the 250mL triangular flask equipped with 150mL liquid submerged culture base, triangular flask is 50 revs/min at rotating speed, temperature 35 DEG C
Under the conditions of, 18h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 10 under the conditions of 130 DEG C
Min prepares, and containing wheat bran 5g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 20g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 1% by volume, it is 35 DEG C in temperature, stirs
Mixing rotating speed is 160 revs/min, is the ventilation of 0.2:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 96h, make Cordyceps seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 130 DEG C
10 min prepare, and containing wheat bran 5g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 20g；
(4) solid fermentation is cultivated: access the Cordyceps seed tank strain obtained by step (3) equipped with general buck wheat
(Fagopyrum esculentum) in solid fermentation culture medium, and described Cordyceps seed tank strain and solid fermentation culture medium
Being the inoculum concentration inoculation of 10% by weight, temperature 20 DEG C, humidity 80% is fermented 40 days, and wherein every 6 days, stirring once, is made
Obtain solid fermentation thing；Described solid fermentation culture medium is that sterilizing 360 min prepares under the conditions of 130 DEG C, and solid medium is pressed
Every kilogram pulverize Semen Fagopyri Esculenti add the solid matrix raw material such as 200g rice, 100g Semen Tritici aestivi, 100g Semen Maydis and 700g Sorghum vulgare Pers. and prepare；
Raw material in described solid fermentation culture medium mixes with water by weight the ratio for 1:1.1；
(5) by the solid fermentation thing obtained by step (4) after 120 DEG C dry 10 hours, it is crushed to below 100 mesh, packaging
Obtain edible functional food；Described functional food contains the flavone of 2mg/g butt matter, 10mg/g butt matter
Cordyceps polysaccharide, the cordycepin of 1mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-spoke
Penetrate and suppress the effects such as tumor；
(6) extract separate: by obtained by step (4) or (5) Cordyceps convert Semen Fagopyri Esculenti produce functional food respectively through
Extract, respectively obtain the product containing flavone, cordycepin and Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 60% of 5 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 25%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 5 times of volumes of mycelium weight
Water, 80 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.5 times
95% ethanol, under the conditions of 4 DEG C crystallize 5 hours, filter, filtrate continuously add extract raffinate volume 2.5 times 95% ethanol,
Crystallizing 5 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 20%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 10 times of volumes of functional food weight
Being 60% ethanol, 60 DEG C are incubated 4 hours；Filtering, the volume by volume concentration of 10 times of volumes that residue adds residue weight is 60% wine
Essence, 60 DEG C are incubated 4 hours, are repeated 2 times；
The E2 supernatant macroporous resin AB-8 type static adsorption 5 hours of 1/50 volume, adsorbs the washing of rear 10 times of resin volumes
Washing, install in chromatographic column, with 20% ethanol desorbing of 10 times of resin volumes, stripping liquid is incorporated in temperature 20 DEG C, is concentrated in vacuo supreme
The 1/100 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 160 DEG C, and air outlet temperature 100 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 70% in cordycepin yeast powder.
Animal experiment proves, contains glycoside soap, cordycepin and Cordyceps polysaccharide functional food respectively and all has regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 2
A kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti specifically includes following steps:
(1) test tube amplification culture: by Cordyceps fungus (Cordyceps sinensis) CICC 50002 test tube slant strain cuts
Become 3 × 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 4 days for 35 DEG C, prepare test tube slant strain,
4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 3 pieces
Being inoculated in the 250mL triangular flask equipped with 20mL liquid submerged culture base, triangular flask is 200 revs/min at rotating speed, temperature 20 DEG C
Under the conditions of, 86h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 60 under the conditions of 100 DEG C
Min prepares, and containing bran 20g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 5g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 20% by volume, it is 20 DEG C in temperature, stirs
Mixing rotating speed is 50 revs/min, is 0.2～1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of ventilation, cultivate 18h, make Cordyceps seed tank strain；Described seed tank amplification culture base is under the conditions of 100 DEG C
Sterilizing 60min prepares, and containing wheat bran 20g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 5g；
(4) solid fermentation cultivate: by obtained by step (3) Cordyceps seed tank strain access equipped with Radix Et Rhizoma Fagopyri Tatarici (Fagopyrum tartaricumGaertn) in solid fermentation culture medium, and described Cordyceps seed tank strain presses weight with solid fermentation culture medium
Amount is inoculated than the inoculum concentration being 2%, and temperature 35 DEG C, humidity 80% is fermented 20 days, and wherein every 3 days, stirring once, prepares solid
Fermented product；Described solid fermentation culture medium is that sterilizing 90min prepares under the conditions of 100 DEG C, and solid medium is by every kilogram of powder
Broken Semen Fagopyri Esculenti adds the solid matrix raw materials such as 400g rice, 300g Semen Tritici aestivi, 300g Semen Maydis and 300g Sorghum vulgare Pers. and prepares；Described solid
Raw material in fermentation medium mixes with water by weight the ratio for 1:1.5；
(5) by the solid fermentation thing obtained by step (4) after 80 DEG C dry 10 hours, it is crushed to below 100 mesh, packs
To edible functional food；Described functional food contains the flavone of 50mg/g butt matter, 100mg/g butt matter
Cordyceps polysaccharide, the cordycepin of 20mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-
The effects such as radiation and suppression tumor；
(6) separation is extracted: passed through by the functional food that the Cordyceps conversion Semen Fagopyri Esculenti obtained by step (4) or (5) produces and extract,
Respectively obtain containing flavone and the product of cordycepin and the product of Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 100% of 20 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 60%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 20 times of volumes of mycelium weight
Distilled water, 100 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.5 times
95% ethanol, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extract raffinate volume 3.5 times 95%
Ethanol, crystallizes 18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 60%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 40 times of volumes of functional food weight
Being 100% ethanol, 90 DEG C are incubated 6 hours；Filtering, the volume by volume concentration of 40 times of volumes that residue adds residue weight is 100%
Ethanol, 90 DEG C are incubated 6 hours, are repeated 3 times；
The E2 supernatant macroporous resin D-113 type static adsorption 20 hours of 1/5 volume, adsorbs the washing of rear 20 times of resin volumes
Washing, install in chromatographic column, with 80% ethanol desorbing of 20 times of resin volumes, stripping liquid is incorporated in temperature 50 C, is concentrated in vacuo supreme
The 1/50 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 190 DEG C, and air outlet temperature 120 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 30% in cordycepin yeast powder.
Animal experiment proves, contains glycoside soap, cordycepin and Cordyceps polysaccharide functional food respectively and all has regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 3
A kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti specifically includes following steps:
(1) test tube amplification culture: by Cordyceps (Cordyceps militaris) CICC 14015 test tube slant strain is cut into 3
× 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 10 days for 28 DEG C, prepare test tube slant strain, should
4 DEG C, test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 7 pieces
Being inoculated in the 250mL triangular flask equipped with 80mL liquid submerged culture base, triangular flask is 120 revs/min at rotating speed, temperature 28 DEG C
Under the conditions of, 57h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 35 under the conditions of 120 DEG C
Min prepares, and containing wheat bran 13g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 13g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 10% by volume, it is 28 DEG C in temperature, stirs
Mixing rotating speed is 110 revs/min, is the ventilation of 1:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of, cultivate 57h, make Cordyceps seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 120 DEG C
35min prepares, and containing wheat bran 13g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 13g；
(4) solid fermentation cultivate: by obtained by step (3) Cordyceps seed tank strain access equipped with Rhizoma Fagopyri Dibotryis (Fagopyrum cymosumL.) in solid fermentation culture medium, and described Cordyceps seed tank strain with solid fermentation culture medium is by weight
The inoculum concentration inoculation of 6%, temperature 28 DEG C, humidity 80% is fermented 30 days, and wherein every 4.5 days, stirring once, prepares solid fermentation
Thing；Described solid fermentation culture medium is that sterilizing 260 min prepares under the conditions of 120 DEG C, and solid medium is by every kilogram of pulverizing
Semen Fagopyri Esculenti add the solid matrix raw material such as 300g rice, 200g Semen Tritici aestivi, 200g Semen Maydis and 500g Sorghum vulgare Pers. and prepare；Described solid is sent out
Raw material in ferment culture medium mixes with water by weight the ratio of 1:0.6；
(5) solid fermentation thing is after 100 DEG C dry 17 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the flavone of 27.5mg/g butt matter, the Cordyceps polysaccharide of 54mg/g butt matter, 11mg/g
The cordycepin of butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc.
Effect；
(6) extract separate: by obtained by step (4) or (5) Cordyceps convert Semen Fagopyri Esculenti produce functional food respectively through
Extract, obtain containing flavone and the product of cordycepin and the product of Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 80% of 12 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 11 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 43%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 13 times of volumes of mycelium weight
Distilled water, 90 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 12 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3 times, in 4
Crystallizing 12 hours under the conditions of DEG C, then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 41%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 30 times of volumes of functional food weight
Being 90% ethanol, 70 DEG C are incubated 5 hours；Filtering, the volume by volume concentration of 20 times of volumes that residue adds residue weight is 90% wine
Essence, 70 DEG C are incubated 5 hours, repeat 2-3 time；
The E2 supernatant macroporous resin D-152 type static adsorption 8 hours of 1/40 volume, adsorbs the washing of rear 12 times of resin volumes
Washing, install in chromatographic column, with 40% ethanol desorbing of 12 times of resin volumes, stripping liquid is incorporated in temperature 30 DEG C, is concentrated in vacuo supreme
The 1/90 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 170 DEG C, and air outlet temperature 110 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 50% in cordycepin yeast powder.
Animal experiment proves, contains glycoside soap, cordycepin and Cordyceps polysaccharide functional food respectively and all has regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor..
Embodiment 4
A kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti specifically includes following steps:
(1) test tube amplification culture: by Cordyceps fungus (Cordyceps sinensis) CICC 50002 test tube slant strain cuts
Become 3 × 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 12 days for 23 DEG C, prepare test tube slant strain,
4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 8 pieces
Being inoculated in the 250mL triangular flask equipped with 30mL liquid submerged culture base, triangular flask is 75 revs/min at rotating speed, temperature 20～35
Under conditions of DEG C, 30h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing under the conditions of 100 DEG C
55 min prepare, and containing wheat bran 8g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 12g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 4% by volume, it is 25 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 0.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 35h, make Cordyceps seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 100 DEG C
55 min prepare, and containing wheat bran 8g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 15g；
(4) solid fermentation is cultivated: access the Cordyceps seed tank strain obtained by step (3) equipped with general buck wheat
(Fagopyrum esculentum) in solid fermentation culture medium, and described Cordyceps seed tank strain and solid fermentation culture medium
Being the inoculum concentration inoculation of 4% by weight, temperature 22 DEG C, humidity 80% is fermented 25 days, and wherein every 3 days, stirring once, prepares
Solid fermentation thing；Described solid fermentation culture medium is that sterilizing 100 min prepares under the conditions of 100 DEG C, and solid medium is by every
Kilogram pulverize Semen Fagopyri Esculenti add the solid matrix raw material such as 250g rice, 150g Semen Tritici aestivi, 150g Semen Maydis and 600g Sorghum vulgare Pers. and prepare；Institute
The ratio stating the 1:1.0 by weight of the raw material in solid fermentation culture medium mixes with water；
(5) by the solid fermentation thing obtained by step (4) after 90 DEG C dry 22 hours, it is crushed to below 100 mesh, packs
To edible functional food；Described functional food contains the flavone of 45mg/g butt matter, 90mg/g butt matter
Cordyceps polysaccharide, the cordycepin of 5mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-spoke
Penetrate and suppress the effects such as tumor；
(6) separation is extracted: passed through by the functional food that the Cordyceps conversion Semen Fagopyri Esculenti obtained by step (4) or (5) produces and extract,
Respectively obtain containing flavone and the product of cordycepin and the product of Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 70% of 7 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 6 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 31%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 7 times of volumes of mycelium weight
Water, 85 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.7 times
95% ethanol, under the conditions of 4 DEG C crystallize 7 hours, filter, filtrate continuously add extract raffinate volume 2.8 times 95% ethanol,
Crystallizing 7 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 31%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 30 times of volumes of functional food weight
Being 70% ethanol, 80 DEG C are incubated 4 hours；Filtering, the volume by volume concentration of 30 times of volumes that residue adds residue weight is 70% wine
Essence, 80 DEG C are incubated 4 hours, are repeated 3 times；
The E2 supernatant macroporous resin HD-2 type static adsorption 12 hours of 1/30 volume, adsorbs the washing of rear 15 times of resin volumes
Washing, install in chromatographic column, with 70% ethanol desorbing of 15 times of resin volumes, stripping liquid is incorporated in temperature 40 DEG C, is concentrated in vacuo supreme
The 1/80 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 180 DEG C, and air outlet temperature 100 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 40% in cordycepin yeast powder.
Animal experiment proves, contains glycoside soap, cordycepin and Cordyceps polysaccharide functional food respectively and all has regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor..
Embodiment 5
A kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti specifically includes following steps:
(1) test tube amplification culture: by Cordyceps (Cordyceps militaris) CICC 14015 test tube slant strain is cut into 3
× 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 6 days for 32 DEG C, prepare test tube slant strain, this examination
4 DEG C of pipe inclined-plane saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 40mL liquid submerged culture base, triangular flask is 160 revs/min at rotating speed, temperature 30 DEG C
Under the conditions of, 36h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 28 under the conditions of 128 DEG C
Min prepares, and containing wheat bran 6g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 20g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 30 DEG C in temperature, stirs
Mixing rotating speed is 160 revs/min, is the ventilation of 0.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 68h, make Cordyceps seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 128 DEG C
28 min prepare, and containing wheat bran 15g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 7g；
(4) solid fermentation cultivate: by obtained by step (3) Cordyceps seed tank strain access equipped with Radix Et Rhizoma Fagopyri Tatarici (Fagopyrum tartaricumGaertn) in solid fermentation culture medium, and described Cordyceps seed tank strain presses weight with solid fermentation culture medium
Amount is inoculated than the inoculum concentration being 6%, and temperature 30 DEG C, humidity 80% is fermented 28 days, and wherein every 5 days, stirring once, prepares solid
Fermented product；Described solid fermentation culture medium is that sterilizing 320 min prepares under the conditions of 128 DEG C, and solid medium is by every kilogram
Pulverize Semen Fagopyri Esculenti add the solid matrix raw material such as 350g rice, 250g Semen Tritici aestivi, 250g Semen Maydis and 400g Sorghum vulgare Pers. and prepare；Described solid
Raw material in body fermentation medium mixes with water by weight the ratio of 1:0.6；
(5) solid fermentation thing is after 90 DEG C dry 18 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the flavone of 10mg/g butt matter, and the Cordyceps polysaccharide of 24mg/g butt matter, 16mg/g are dry
The cordycepin of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor
Effect；
(6) separation is extracted: passed through by the functional food that the Cordyceps conversion Semen Fagopyri Esculenti obtained by step (4) or (5) produces and extract,
Respectively obtain containing flavone and the product of cordycepin and the product of Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 90% of 17 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 15 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 25%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 18 times of volumes of mycelium weight
Distilled water, 95 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.4 times
95% ethanol, under the conditions of 4 DEG C crystallize 16 hours, filter, filtrate continuously add extract raffinate volume 3.2 times 95% second
Alcohol, crystallizes 16 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 52%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 15 times of volumes of functional food weight
Being 85% ethanol, 75 DEG C are incubated 6 hours；Filtering, the volume by volume concentration of 15 times of volumes that residue adds residue weight is 85% wine
Essence, 75 DEG C are incubated 6 hours, are repeated 2 times；
E2 supernatant macroporous resin HZD-3 type static adsorption 5-20 hour of 1/10 volume, adsorbs rear 18 times of resin volumes
Water washs, and installs in chromatographic column, and with 75% ethanol desorbing of 18 times of resin volumes, stripping liquid is incorporated in temperature 50 C, is concentrated in vacuo
To supernatant volume 1/40；
E3 concentrated solution is by regulation flow speed control air inlet temperature 165 DEG C, and air outlet temperature 118 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 61% in cordycepin yeast powder.
Animal experiment proves, contains glycoside soap, cordycepin and Cordyceps polysaccharide functional food respectively and all has regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 6
A kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti specifically includes following steps:
(1) test tube amplification culture: by Cordyceps fungus (Cordyceps sinensis) CICC 50002 test tube slant strain cuts
Become 3 × 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 5 days for 32 DEG C, prepare test tube slant strain,
4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 20mL liquid submerged culture base, triangular flask is 170 revs/min at rotating speed, temperature 25 DEG C
Under the conditions of, 60h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 50 under the conditions of 110 DEG C
Min prepares, and containing wheat bran 16g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 8g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 25 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 1.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 29h, make Cordyceps seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 110 DEG C
50 min prepare, and containing wheat bran 14g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 10g；
(4) solid fermentation cultivate: by obtained by step (3) Cordyceps seed tank strain access equipped with Rhizoma Fagopyri Dibotryis (Fagopyrum cymosumL.) in solid fermentation culture medium, and described Cordyceps seed tank strain with solid fermentation culture medium is by weight
The inoculum concentration inoculation of 6%, temperature 25 DEG C, humidity 80% is fermented 26 days, and wherein every 5 days, stirring once, prepares solid fermentation
Thing；Described solid fermentation culture medium is that sterilizing 250 min prepares under the conditions of 110 DEG C, and solid medium is by every kilogram of pulverizing
Semen Fagopyri Esculenti add the solid matrix raw material such as 400g rice, 150g Semen Tritici aestivi, 150g Semen Maydis and 500g Sorghum vulgare Pers. and prepare；Described solid is sent out
Raw material in ferment culture medium mixes with water by weight the ratio of 1:0.8；
(5) by the solid fermentation thing obtained by step (4) after 110 DEG C dry 16 hours, it is crushed to below 100 mesh, packaging
Obtain edible functional food；Described functional food contains the flavone of 15mg/g butt matter, 56mg/g butt matter
Cordyceps polysaccharide, the cordycepin of 18mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-
The effects such as radiation and suppression tumor；
(6) separation is extracted: passed through by the functional food that the Cordyceps conversion Semen Fagopyri Esculenti obtained by step (4) or (5) produces and extract,
Respectively obtain containing flavone and the product of cordycepin and the product of Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 63% of 11 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 12 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 55%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 13 times of volumes of mycelium weight
Distilled water, 90 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 13 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3.0 times,
Crystallizing 13 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 45%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 40 times of volumes of functional food weight
Being 60% ethanol, 90 DEG C are incubated 4 hours；Filtering, the volume by volume concentration of 40 times of volumes that residue adds residue weight is 60% wine
Essence, 90 DEG C are incubated 4 hours, are repeated 3 times；
The E2 supernatant macroporous resin HZD-5 type static adsorption 5 hours of 1/5 volume, adsorbs the washing of rear 20 times of resin volumes
Washing, install in chromatographic column, with 80% ethanol desorbing of 20 times of resin volumes, stripping liquid is incorporated in temperature 50 C, is concentrated in vacuo supreme
The 1/100 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 190 DEG C, and air outlet temperature 100 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 37% in cordycepin yeast powder.
Animal experiment proves, contains glycoside soap, cordycepin and Cordyceps polysaccharide functional food respectively and all has regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 7
A kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti specifically includes following steps:
(1) test tube amplification culture: by Cordyceps (Cordyceps militaris) CICC 14015 test tube slant strain is cut into 3
× 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 13 days for 23 DEG C, prepare test tube slant strain, should
4 DEG C, test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 10 pieces
Being inoculated in the 250mL triangular flask equipped with 130mL liquid submerged culture base, triangular flask is 150 revs/min at rotating speed, temperature 23 DEG C
Under conditions of, 36h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 53 under the conditions of 115 DEG C
Min prepares, and containing wheat bran 9g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 20g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 21 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 0.2:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 81h, make Cordyceps seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 115 DEG C
53 min prepare, and containing wheat bran 19g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 18g；
(4) solid fermentation is cultivated: access the Cordyceps seed tank strain obtained by step (3) equipped with general buck wheat
(Fagopyrum esculentum) in solid fermentation culture medium, and described Cordyceps seed tank strain and solid fermentation culture medium
Being the inoculum concentration inoculation of 4% by weight, temperature 21 DEG C, humidity 80% is fermented 34 days, and wherein every 6 days, stirring once, prepares
Solid fermentation thing；Described solid fermentation culture medium is that sterilizing 250 min prepares under the conditions of 110 DEG C, and solid medium is by every
Kilogram pulverize Semen Fagopyri Esculenti add the solid matrix raw material such as 400g rice, 150g Semen Tritici aestivi, 250g Semen Maydis and 400g Sorghum vulgare Pers. and prepare；Institute
The ratio stating the 1:1.0 by weight of the raw material in solid fermentation culture medium mixes with water；
(5) solid fermentation thing is after 115 DEG C dry 16 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the flavone of 50mg/g butt matter, the Cordyceps polysaccharide of 100mg/g butt matter, 1mg/g
The cordycepin of butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc.
Effect；
(6) separation is extracted: passed through by the functional food that the Cordyceps conversion Semen Fagopyri Esculenti obtained by step (4) or (5) produces and extract,
Respectively obtain containing flavone and the product of cordycepin and the product of Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 75% of 9 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 10 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 38%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 10 times of volumes of mycelium weight
Distilled water, 88 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.8 times
95% ethanol, under the conditions of 4 DEG C crystallize 10 hours, filter, filtrate continuously add extract raffinate volume 2.9 times 95% second
Alcohol, crystallizes 10 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 38%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 10 times of volumes of functional food weight
Being 100% ethanol, 60 DEG C are incubated 6 hours；Filtering, the volume by volume concentration of 10 times of volumes that residue adds residue weight is 100%
Ethanol, 60 DEG C are incubated 6 hours, are repeated 2 times；
The E2 supernatant macroporous resin D-152 type static adsorption 20 hours of 1/50-1/5 volume, adsorbs rear 16 times of resin volumes
Water washing, install in chromatographic column, with 60% ethanol desorbing of 16 times of resin volumes, stripping liquid is incorporated in temperature 45 C, and vacuum is dense
It is reduced to the 1/100 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 170 DEG C, and air outlet temperature 110 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 45% in cordycepin yeast powder.
Animal experiment proves, contains glycoside soap, cordycepin and Cordyceps polysaccharide functional food respectively and all has regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 8
A kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti specifically includes following steps:
(1) test tube amplification culture: by Cordyceps fungus (Cordyceps sinensis) CICC 50002 test tube slant strain cuts
Become 3 × 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 10 days for 29 DEG C, prepare test tube slant strain,
4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 8 pieces
Being inoculated in the 250mL triangular flask equipped with 60mL liquid submerged culture base, triangular flask is 160 revs/min at rotating speed, temperature 33 DEG C
Under the conditions of, 80h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 30 under the conditions of 120 DEG C
Min prepares, and containing wheat bran 19g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 19g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 18% by volume, it is 33 DEG C in temperature, stirs
Mixing rotating speed is 140 revs/min, is the ventilation of 1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 90h, make Cordyceps seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 120 DEG C
30 min prepare, and containing wheat bran 18g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 18g；
(4) solid fermentation cultivate: by obtained by step (3) Cordyceps seed tank strain access equipped with Radix Et Rhizoma Fagopyri Tatarici (Fagopyrum tartaricumGaertn) in solid fermentation culture medium, and described Cordyceps seed tank strain presses weight with solid fermentation culture medium
Amount is inoculated than the inoculum concentration being 9%, and temperature 33 DEG C, humidity 80% is fermented 37 days, and wherein every 6 days, stirring once, prepares solid
Fermented product；Described solid fermentation culture medium is that sterilizing 350 min prepares under the conditions of 105 DEG C, and solid medium is by every kilogram
Pulverize Semen Fagopyri Esculenti add the solid matrix raw material such as 280g rice, 220g Semen Tritici aestivi, 270g Semen Maydis and 380g Sorghum vulgare Pers. and prepare；Described solid
Raw material in body fermentation medium mixes with water by weight the ratio of 1:1.4；
(5) solid fermentation thing is after 85 DEG C dry 23 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the flavone of 45mg/g butt matter, and the Cordyceps polysaccharide of 94mg/g butt matter, 3mg/g are dry
The cordycepin of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor
Effect；
(6) separation is extracted: passed through by the functional food that the Cordyceps conversion Semen Fagopyri Esculenti obtained by step (4) or (5) produces and extract,
Respectively obtain containing flavone and the product of cordycepin and the product of Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 97% of 16 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 15 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 26%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 18 times of volumes of mycelium weight
Distilled water, 95 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.4 times
95% ethanol, under the conditions of 4 DEG C crystallize 16 hours, filter, filtrate continuously add extract raffinate volume 3.2 times 95% second
Alcohol, crystallizes 6 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 57%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 25 times of volumes of functional food weight
Being 65% ethanol, 82 DEG C are incubated 5 hours；Filtering, the volume by volume concentration of 25 times of volumes that residue adds residue weight is 65% wine
Essence, 82 DEG C are incubated 5 hours, are repeated 2 times；
E2 supernatant macroporous resin HD-2 type static adsorption 5-20 hour of 1/8 volume, adsorbs the water of rear 16 times of resin volumes
Washing, installs in chromatographic column, and with 48% ethanol desorbing of 16 times of resin volumes, stripping liquid is incorporated in temperature 36 DEG C, is concentrated in vacuo to
The 1/70 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 180 DEG C, and air outlet temperature 118 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 67% in cordycepin yeast powder.
Animal experiment proves, flavone and cordycepin functional food and Cordyceps polysaccharide functional food all have regulation blood
The effects such as fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 9
A kind of method of functional food utilizing Cordyceps to convert Semen Fagopyri Esculenti specifically includes following steps:
(1) test tube amplification culture: by Cordyceps (Cordyceps militaris) CICC 14015 test tube slant strain is cut into 3
× 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 6 days for 24 DEG C, prepare test tube slant strain, this examination
4 DEG C of pipe inclined-plane saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 24mL liquid submerged culture base, triangular flask is 58 revs/min at rotating speed, temperature 24 DEG C
Under the conditions of, 20h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 59 under the conditions of 100 DEG C
Min prepares, and containing wheat bran 5g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 5g；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 3% by volume, it is 24 DEG C in temperature, stirs
Mixing rotating speed is 58 revs/min, is the ventilation of 1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of, cultivate 25h, make Cordyceps seed tank strain；Described seed tank amplification culture base is sterilizing 59 under the conditions of 100 DEG C
Min prepares, and containing wheat bran 5g in every liter of liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 6g；
(4) solid fermentation cultivate: by obtained by step (3) Cordyceps seed tank strain access equipped with or Rhizoma Fagopyri Dibotryis
(Fagopyrum cymosumL.) in solid fermentation culture medium, and described Cordyceps seed tank strain and solid fermentation culture medium
Being the inoculum concentration inoculation of 3% by weight, temperature 24 DEG C, humidity 80% is fermented 23 days, and wherein every 3 days, stirring once, prepares
Solid fermentation thing；Described solid fermentation culture medium is that sterilizing 150 min prepares under the conditions of 100 DEG C, and solid medium is by every
Kilogram pulverize Semen Fagopyri Esculenti add the solid matrix raw material such as 360g rice, 240g Semen Tritici aestivi, 160g Semen Maydis and 540g Sorghum vulgare Pers. and prepare；Institute
The ratio stating the 1:1.3 by weight of the raw material in solid fermentation culture medium mixes with water；
(5) solid fermentation thing is after 110 DEG C dry 13 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the flavone of 2mg/g butt matter, and the Cordyceps polysaccharide of 10mg/g butt matter, 20mg/g are dry
The cordycepin of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor
Effect；
(6) separation is extracted: passed through by the functional food that the Cordyceps conversion Semen Fagopyri Esculenti obtained by step (4) or (5) produces and extract,
Respectively obtain containing flavone and the product of cordycepin and the product of Cordyceps polysaccharide.
It should be noted that and comprise the steps: from the method for the product containing flavone described in step (6)
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 81% of 15 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 14 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing the functional food of flavone
Product, this food flavones content is 57%.
In the present embodiment, comprise the steps: from the method containing Cordyceps polysaccharide product described in step (6)
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 13 times of volumes of mycelium weight
Distilled water, 92 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 12 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3.1 times,
Crystallizing 12 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide purity 42%；
In the present embodiment, comprise the steps: from the method containing cordycepin yeast powder described in step (6)
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and adds the volume by volume concentration of 28 times of volumes of functional food weight
Being 80% ethanol, 76 DEG C are incubated 6 hours；Filtering, the volume by volume concentration of 28 times of volumes that residue adds residue weight is 80% wine
Essence, 76 DEG C are incubated 6 hours, are repeated 2 times；
E2 supernatant macroporous resin HZD-3 type static adsorption 5-20 hour of 1/5 volume, adsorbs the water of rear 17 times of resin volumes
Washing, installs in chromatographic column, and with 30% ethanol desorbing of 17 times of resin volumes, stripping liquid is incorporated in temperature 21 DEG C, is concentrated in vacuo to
The 1/50 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 169 DEG C, and air outlet temperature 108 DEG C carries out spray drying and obtains worm
Grass element yeast powder.Cordycepin purity 34% in cordycepin yeast powder.
Animal experiment proves, contains glycoside soap, cordycepin and Cordyceps polysaccharide functional food respectively and all has regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Schematically being described the present invention and embodiment thereof above, this description does not has restricted, actual knot
Structure is not limited thereto.So, if those of ordinary skill in the art is enlightened by it, without departing from the invention objective
In the case of, design the frame mode similar to this technical scheme and embodiment without creative, all should belong to the present invention's
Protection domain.
Claims (10)
1. one kind utilizes Cordyceps to convert the method that Semen Fagopyri Esculenti produces functional food, it is characterised in that described preparation method is with buckwheat
Wheat and rice, Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers. etc. are solid matrix, with Cordyceps as starting strain, sequentially pass through test tube amplification culture,
Liquid submerged culture and seed tank amplification culture, solid fermentation cultivate, dry, pulverize and prepared by the technique such as packaging；Described merit
Energy property food contains the flavone of 2～50mg/g butt matter, the Cordyceps polysaccharide of 10～100mg/g butt matter, 1～20mg/g butt matter
Cordycepin, there is the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor；Described functional food is permissible
Extract its active component flavone, Cordyceps polysaccharide and cordycepin, be used for producing treatment reduce blood fat, blood glucose and blood pressure, radioprotective and
Medicine or the functional foods such as the tablet of suppression tumor or capsule.
A kind of Cordyceps the most according to claim 1 convert Semen Fagopyri Esculenti produce functional food method, it is characterised in that
The buckwheat used include general buck wheat (Fagopyrum esculentum), Radix Et Rhizoma Fagopyri Tatarici (Fagopyrum tartaricumGaertn) or Rhizoma Fagopyri Dibotryis (Fagopyrum cymosumL.)；The Cordyceps strain used includes Cordyceps
(Cordyceps militaris) or Cordyceps fungus (Cordyceps sinensis)。
A kind of Cordyceps the most according to claim 1 converts the method that Semen Fagopyri Esculenti produces functional food, it is characterised in that institute
State preparation method to comprise the steps:
A1 test tube amplification culture: Cordyceps slant strains be inoculated in potato dextrose medium and cultivate, prepares worm
Grass bacterium test tube slant strain；
A2 liquid submerged culture: the Cordyceps test tube slant strain obtained by step A1 is inoculated into equipped with liquid submerged culture base
Shaking flask in, cultivate, prepare Cordyceps liquid shaking bottle strain；
A3 seed tank amplification culture: be inoculated in seed tank culture base by the Cordyceps liquid shaking bottle strain obtained by step A2
Row is cultivated, and makes Cordyceps seed tank strain；
A4 solid fermentation is cultivated: is inoculated in solid medium by the Cordyceps seed tank strain obtained by step A3, and mixes
Uniformly, carry out fermentation culture, prepare Cordyceps solid fermentation thing；
A5 packs: Cordyceps solid fermentation thing converts the functional food of Semen Fagopyri Esculenti through drying, pulverize, pack prepared Cordyceps.
A kind of Cordyceps the most according to claim 3 converts the method that Semen Fagopyri Esculenti produces functional food, it is characterised in that institute
State preparation method specific as follows:
B1 test tube amplification culture: slant strains in Cordyceps test tube is cut into 3 × 3 mm fritter strains, inoculates a fritter to test tube
In slant medium, cultivating 4～15 days for 20～35 DEG C, prepare test tube slant strain, 4 DEG C, this test tube slant saves backup；
B2 liquid submerged culture: the test tube slant strain obtained by step B1 is cut into 3 × 3 mm fritter strains, picking 3～10
Block is inoculated in the 250mL triangular flask equipped with 20～150mL liquid submerged culture bases, triangular flask rotating speed be 50～200 turns/
Point, under conditions of temperature 20～35 DEG C, 18-86h cultivated by shaking table, makes liquid shaking bottle strain；
B3 seed tank amplification culture: the liquid shaking bottle strain obtained by step B2 is inoculated in seed tank amplification culture base, and
Described liquid shaking bottle strain and seed tank amplification culture base are the inoculum concentration inoculation of 1～20% by volume, are 20～35 in temperature
DEG C, speed of agitator is 50～160 revs/min, is 0.2 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
～under the conditions of the ventilation of 1.8:1, cultivate 18～96h, make Cordyceps seed tank strain；
B4 solid fermentation is cultivated: accessed in solid fermentation culture medium by the Cordyceps seed tank strain obtained by step B3, and institute
State the inoculum concentration inoculation that Cordyceps seed tank strain and solid fermentation culture medium are 2～10% by weight, in temperature 20～35
DEG C, humidity 80% is fermented 5～20 days, and wherein every 3～6 days, stirring once, prepares solid fermentation thing；
B5 solid fermentation thing, after 80～120 DEG C dry 10～24 hours, is crushed to below 100 mesh, is packaged to be and can eat
Functional food.
A kind of Cordyceps the most according to claim 4 converts the method that Semen Fagopyri Esculenti produces functional food, it is characterised in that step
Liquid submerged culture base described in rapid B2 is that sterilizing 10～60 min prepares under the conditions of 100～130 DEG C, and every liter of liquid shaking bottle
Containing wheat bran 5～20g in culture medium, pulverizing the raw material of Semen Fagopyri Esculenti 5～20g,
The base of seed tank amplification culture described in step B3 is that sterilizing 10～60 min prepares under the conditions of 100～130 DEG C, and every liter
Containing wheat bran 5～20g in liquid submerged culture base, pulverizing the raw material of Semen Fagopyri Esculenti 5～20g,
Solid fermentation culture medium described in step B4 is that sterilizing 90～360 min prepares under the conditions of 100～130 DEG C, and every thousand
Gram pulverize Semen Fagopyri Esculenti add 200～400g rice, 100～300g Semen Tritici aestivi, 100～300g Semen Maydis and 700～300g Sorghum vulgare Pers. etc.
Solid matrix raw material.
A kind of Cordyceps the most according to claim 7 converts the method that Semen Fagopyri Esculenti produces functional food, it is characterised in that institute
The ratio stating the 1:0.6～1.5 by weight of the solid matrix in solid fermentation culture medium mixes with water.
A kind of Cordyceps the most according to claim 1 converts the method that Semen Fagopyri Esculenti produces functional food, it is characterised in that step
Convert from Cordyceps described in rapid A4, A5 or step B4, B5 and the functional food that Semen Fagopyri Esculenti produces extracts the method for flavone include
Following steps:
Obtained Cordyceps is converted Semen Fagopyri Esculenti solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
60～the ethanol of 100% of 5～20 times of volumes of filament weight, repeatedly extracts 3～5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3～18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Cordyceps
Element functional food, this food flavones content is 25～60%.
A kind of Cordyceps the most according to claim 1 converts the method that Semen Fagopyri Esculenti produces functional food, it is characterised in that step
Convert, from Cordyceps, the method extracting Cordyceps polysaccharide the functional food that Semen Fagopyri Esculenti produces described in rapid A4, A5 or step B4, B5
Comprise the steps:
Obtained Cordyceps mycelium is dried by D1, is then crushed to 20 mesh, adds 5～20 times of volumes of mycelium weight
Distilled water, 80～100 DEG C are repeatedly extracted 3～5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, add residual volume 0.5～
95% ethanol of 1.5 times, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extraction raffinate volume 2.5～
95% ethanol of 3.5 times, crystallizes 5～18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Cordyceps polysaccharide, Cordyceps polysaccharide
Purity 20～60%.
A kind of Cordyceps the most according to claim 1 converts the method that Semen Fagopyri Esculenti produces functional food, it is characterised in that step
Convert the method bag extracting cordycepin the functional food that Semen Fagopyri Esculenti produces from Cordyceps described in rapid A4, A5 or step B4, B5
Include following steps:
E1 Cordyceps converts Semen Fagopyri Esculenti functional food and pulverizes, and the volume ratio of the 10-40 times of volume adding functional food weight is dense
Degree is 60-100% ethanol, and 60-90 DEG C is incubated 4-6 hour；Filtering, residue adds the volume of 10-40 times of volume of residue weight
Specific concentration is 60-100% ethanol, and 60-90 DEG C is incubated 4-6 hour, repeats 2-3 time；
E2 supernatant macroporous resin static adsorption 5-20 hour of 1/50-1/5 volume, 10-20 times of resin volume after absorption
Water washs, and installs in chromatographic column, and with the 20-80% ethanol desorbing of 10-20 times of resin volume, stripping liquid is incorporated in temperature 20-50
DEG C, it is concentrated in vacuo to the 1/100-1/50 of supernatant volume；
Preferably macroporous resin includes all low pole macroporous resins, such as AB-8 type, D-113 type, D-152 type, HD-2 type, HZD-
3 types, HZD-5 type etc.；
E3 concentrated solution is by regulation flow speed control air inlet temperature 160-190 DEG C, and air outlet temperature 100-120 DEG C carries out spray dried
Dry obtain cordycepin yeast powder；
Cordycepin purity 30～70% in cordycepin yeast powder.
10. converting, according to a kind of Cordyceps described in right 1, the method that Semen Fagopyri Esculenti produces functional food, step C2, D2 and E3 add
Cordyceps polysaccharide that work obtains or cordycepin and flavone, can be processed into tablet and glue according to conventional tablet or capsule preparation method
Capsule, the tablet being processed into and capsule have regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610543868.5A CN106165824A (en) | 2016-07-12 | 2016-07-12 | A kind of method utilizing Cordyceps to convert Semen Fagopyri Esculenti production functional food |
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| CN1148623A (en) * | 1996-10-04 | 1997-04-30 | 海南兆隆实业有限公司 | North cordyceps mycelium fermentation technology |
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| CN103446190A (en) * | 2013-08-27 | 2013-12-18 | 王辉 | Method for producing fermentative cordycep fungal powder by solid fermentation of mixed strains |
| CN104823690A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis and cordyceps millitaris buckwheat |
| CN105105110A (en) * | 2015-08-04 | 2015-12-02 | 江苏大学 | Making method of monascus and tartary buckwheat functional food and application thereof |
| CN105361136A (en) * | 2015-08-04 | 2016-03-02 | 江苏大学 | Preparation method and application of monascus transformed tartary buckwheat fermentation liquor |
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| CN1148623A (en) * | 1996-10-04 | 1997-04-30 | 海南兆隆实业有限公司 | North cordyceps mycelium fermentation technology |
| CN101791329A (en) * | 2010-03-30 | 2010-08-04 | 天津科技大学 | Astragalus waste cordyceps sinensis fermentation technique and application thereof |
| CN103271281A (en) * | 2013-06-09 | 2013-09-04 | 食品行业生产力促进中心 | Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation |
| CN103446190A (en) * | 2013-08-27 | 2013-12-18 | 王辉 | Method for producing fermentative cordycep fungal powder by solid fermentation of mixed strains |
| CN104823690A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis and cordyceps millitaris buckwheat |
| CN105105110A (en) * | 2015-08-04 | 2015-12-02 | 江苏大学 | Making method of monascus and tartary buckwheat functional food and application thereof |
| CN105361136A (en) * | 2015-08-04 | 2016-03-02 | 江苏大学 | Preparation method and application of monascus transformed tartary buckwheat fermentation liquor |
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