CN106174221A - A kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food - Google Patents
A kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food Download PDFInfo
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- CN106174221A CN106174221A CN201610543861.3A CN201610543861A CN106174221A CN 106174221 A CN106174221 A CN 106174221A CN 201610543861 A CN201610543861 A CN 201610543861A CN 106174221 A CN106174221 A CN 106174221A
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- ganoderma
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Biotechnology (AREA)
- Organic Chemistry (AREA)
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- Wood Science & Technology (AREA)
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- Tropical Medicine & Parasitology (AREA)
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Abstract
The invention discloses a kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food, relate to technical field of bioengineering.Described preparation method sequentially passes through test tube amplification culture, liquid submerged culture and seed tank amplification culture, solid fermentation is cultivated, dry and pulverising step prepares and has regulation blood fat, blood glucose and blood pressure, radioprotective and the functional food of suppression tumor.The present invention, with Rhizoma Dioscoreae and rice, Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers. etc. as solid matrix, with Ganoderma for strain by solid fermentation, converts Rhizoma Dioscoreae Saponin, simultaneously synthesizing Ganodenic acid and ganoderan；Obtained product, saponin content 2～20mg/g butt matter, ganoderma polyoses content 10～100mg/g butt matter, ganoderic acid content 1～20mg/g butt matter, can be used for extracting its active component Saponin, ganoderan and Ganodenic acid, produce treatment and reduce blood fat, blood glucose and blood pressure, radioprotective and the tablet of suppression tumor or capsule.
Description
Technical field
The present invention relates to technical field of bioengineering, be specifically related to one and utilize Ganoderma to convert Rhizoma Dioscoreae production functional food
Method.
Background technology
Rhizoma Dioscoreae is the cultigen that can form underground meat tuber in potato preparatory course Wild yam, annual or perennial prehensile rattan
This plant, another name Rhizomadioscoreae, potato damage, RHIIZOMA DIOSCOREAE fr0m Henan of China etc..Rhizoma Dioscoreae is the highly efficient and productive industrial crops of a kind of dietotherapeutic, drying is used as medicine
For tonic invigorator, weakness, chronic enteritis, diabetes etc. there is auxiliary curative effect.Rhizoma Dioscoreae rich in proteins, aminoacid, starch etc.
The bioactive ingredients such as nutrient substance and polysaccharide, saponin, vitamin, allantoin, dopamine, Rhizoma Dioscoreae alkali, polyphenol oxidase.
Compendium of Material Medica Rhizoma Dioscoreae controls weakness, treats five kinds of strain and seven kinds of impairment, only lumbago, heat clearing away and restlessness relieving, reinforcing the heart QI-insufficiency, reach central hole, many accounts,
The effects such as bone and muscle strengthening, kidney tonifying gas, strengthening the spleen and stomach, antidiarrheal dysentery, the saliva that reduces phlegm, profit fur, " Records of Tradition Chinese and Western Medicine in Combination " is recorded Rhizoma Dioscoreae and is had
Only quench one's thirst (diabetes).Modern medicine study proves that Rhizoma Dioscoreae has regulation or enhancing human body immunity, reduces blood pressure and blood fat effect
And antitumor, antioxidation, defying age and mutation etc. act on.
The initial eating method of Rhizoma Dioscoreae be remove the peel, dry, long term storage after pulverizing, generally Rhizoma Dioscoreae powder is made respectively by steaming and decocting
Kind of food or Rhizoma Dioscoreae is directly stewed into soup eat.In the last few years, along with the progress of science and technology, people's exploitation to Rhizoma Dioscoreae
The most gradually to in-depth development, the report about Rhizoma Dioscoreae product R & D is a lot: Rhizoma Dioscoreae convection drying is made full powder, essence
Powder, directly pulls an oar Rhizoma Dioscoreae and makes series beverage, Rhizoma Dioscoreae starch is made ourishing rice flour, and Rhizoma Dioscoreae and pure milk, rice etc. is compound
It is fermented into yam Yogurt and yam wine etc..At present, mountain the effective elements of the medicine extracting directly is out developed into there is health care
Food be increasingly becoming Rhizoma Dioscoreae develop new approaches.But rarely report utilizes medical edible fungal to give birth to Rhizoma Dioscoreae
The research that thing converts.
Inventor, through further investigation and industrialized production test repeatedly, finds out industrially scalable conversion Rhizoma Dioscoreae raw
Produce functional food technology, the present invention no matter from the mode of production and the product of production of product, be all different from conventional patent and
The content of article report, a kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food.
Summary of the invention
, problem to be solved
For the above-mentioned problems in the prior art, the present invention provides one to utilize Ganoderma to convert Rhizoma Dioscoreae and produces functional food
Method, it is with making beating Rhizoma Dioscoreae and rice, Semen Tritici aestivi, Semen Maydis and Sorghum vulgare Pers. as solid matrix, with Ganoderma for strain by solid fermentation,
Convert Rhizoma Dioscoreae, make Ganoderma mycelium have the functional food of pharmacy function, work with Rhizoma Dioscoreae interaction production by fermentation
Skill is simple, and in obtained product, saponin content 2～20mg/g butt matter, ganoderma polyoses content 10～100mg/g butt
Matter, ganoderic acid content 1～20mg/g butt matter.This product can also extract Ganodenic acid further and Saponin, polysaccharide thus prepare
Product there is the pharmacognosy function identical with fermented product.
, technical scheme
In order to solve the problems referred to above, the technical solution adopted in the present invention is as follows:
Described a kind of utilizing Ganoderma to convert the method that Rhizoma Dioscoreae produces functional food, described preparation method is with making beating Rhizoma Dioscoreae, big
Rice, Semen Tritici aestivi, Semen Maydis and Sorghum vulgare Pers. are main matrix, with Ganoderma as starting strain, sequentially pass through test tube amplification culture, liquid shaking bottle training
Support and the step such as seed tank amplification culture, solid fermentation cultivation；Described functional food can prepare soap respectively through extracting again
Glycoside and Ganodenic acid extract, ganoderan extract, wherein in Saponin and Ganodenic acid extract, Saponin and ganoderic acid content be respectively
It is 25～60% and 30～70%, ganoderan purity 20～60%.
Described a kind of Ganoderma convert Rhizoma Dioscoreae produce functional food method, the Rhizoma Dioscoreae raw material bag that the method is used
Include: RHIIZOMA DIOSCOREAE fr0m Henan of China (Dioscorea oppositaThunb), mesa medicine (Dioscorea alataAnd mutation sole sweet potato L.)
(Dioscorea alataL.f. flabella Makino.) and include dioscorea japonica (Dioscorea japonica
Thunb.), brown bud potato (Dioscorea persimilisPrain et Burk.), mountain potato (Dioscorea fordii
Prain et Burk.) or spit of fland Rhizoma Dioscoreae (Dioscorea quinquelabaSoil Rhizoma Dioscoreae, the Rhizoma Dioscoreae such as Thunb.)
(Dioscorea oppositaFoshou) or Fructus Citri Sarcodactylis Rhizoma Dioscoreae (Dioscorea oppositaIron-rod)；The spirit used
Sesame strain include Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum), Ganoderma (Ganoderma lucidum) or Ganoderma (Ganoderma sinensis)。
A kind of Ganoderma converts the method that Rhizoma Dioscoreae produces functional food, carries out as steps described below:
A1 test tube amplification culture: Ganoderma slant strains be inoculated in potato dextrose medium and cultivate, prepares Ganoderma
Test tube slant strain；
A2 liquid submerged culture: the Ganoderma test tube slant strain obtained by step A1 is inoculated into equipped with liquid submerged culture base
In shaking flask, cultivate, prepare ganoderma lucidum liquid shaking flask strain；
A3 seed tank amplification culture: the ganoderma lucidum liquid shaking flask strain obtained by step A2 is inoculated in seed tank culture base and carries out
Cultivate, make Ganoderma seed tank strain；
A4 solid fermentation is cultivated: be inoculated in solid medium by the Ganoderma seed tank strain obtained by step A3, and mixes all
Even, carry out fermentation culture, prepare Ganoderma solid fermentation thing；
A5 packs: Ganoderma solid fermentation thing converts the functional food of Rhizoma Dioscoreae through drying, pulverize, pack prepared Ganoderma.
Preferably, a kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food, carry out as steps described below:
B1 test tube amplification culture: slant strains in Ganoderma test tube is cut into 3 × 3 mm fritter strains, inoculates a fritter oblique to test tube
In the culture medium of face, cultivating 4～15 days for 20～35 DEG C, prepare test tube slant strain, 4 DEG C, this test tube slant saves backup；
B2 liquid submerged culture: the test tube slant strain obtained by step B1 is cut into 3 × 3 mm fritter strains, picking 3～10
Block is inoculated in the 250mL triangular flask equipped with 20～150mL liquid submerged culture bases, triangular flask rotating speed be 50～200 turns/
Point, under conditions of temperature 20～35 DEG C, cultivate 18-86h, make liquid shaking bottle strain；
B3 seed tank amplification culture: the liquid shaking bottle strain obtained by step B2 is inoculated in seed tank amplification culture base, and
Described liquid shaking bottle strain and seed tank amplification culture base are the inoculum concentration inoculation of 1～20% by volume, are 20～35 in temperature
DEG C, speed of agitator is 50～160 revs/min, is 0.2 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
～under the conditions of the ventilation of 1.8:1, cultivate 18～96h, make Ganoderma seed tank strain；
B4 solid fermentation is cultivated: accessed in solid fermentation culture medium by the Ganoderma seed tank strain obtained by step B3, and described
Ganoderma seed tank strain and solid fermentation culture medium are the inoculum concentration inoculation of 2～10% by weight, temperature 20～35 DEG C, wet
Degree 80% fermentation 5～20 days, wherein every 3～6 days, stirring once, prepares solid fermentation thing；
B5 solid fermentation thing, after 80～120 DEG C dry 10～24 hours, is crushed to below 100 mesh, is packaged to be and can eat
Functional food；
Step A4, A5 or step B4, B5 functional food carry out the extracting method of Ganodenic acid and Saponin and comprise the steps:
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
60～the ethanol of 100% of 5～20 times of volumes of body weight, repeatedly extracts 3～5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3～18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganoderma
Acid function food, this food Saponin and ganoderic acid content are respectively 25～60% and 30～70%.
Step A4, A5 or step B4, B5 functional food carry out the extraction of ganoderan and comprise the steps:
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 5～20 times of volumes of mycelium weight
Distilled water, 80～100 DEG C are repeatedly extracted 3～5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, add residual volume 0.5～
95% ethanol of 1.5 times, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extraction raffinate volume 2.5～
95% ethanol of 3.5 times, crystallizes 5～18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan
Purity 20～60%.
Preferably, liquid submerged culture base described in step B2 is sterilizing 10～60 min system under the conditions of 100～130 DEG C
, and containing wheat bran 5～20g, the raw material of the Rhizoma Dioscoreae 5～20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base.
Preferably, the base of seed tank amplification culture described in step B3 is sterilizing 10～60 min system under the conditions of 100～130 DEG C
, and containing wheat bran 5～20g, the raw material of the Rhizoma Dioscoreae 5～20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base.
Preferably, solid fermentation culture medium described in step B4 is sterilizing 90～360 min under the conditions of 100～130 DEG C
Prepare, and 10000 revs/min every kilogram homogenate Rhizoma Dioscoreae add add 200～400g rice, 100～300g Semen Tritici aestivi, 100～
The solid matrix raw materials such as 300g Semen Maydis and 700～300g Sorghum vulgare Pers..
Preferably, the raw material in described solid fermentation culture medium mixes with water by weight the ratio for 1:0.6～1.5.
, beneficial effect
Compared to prior art, the invention have the benefit that
(1) present invention is on the basis of drawing herb fermenting pharmaceutical technology, in conjunction with the advantage of modern solid-state fermentation, uses solid-state to send out
Ferment converts Rhizoma Dioscoreae, overcomes the substrate (active component of the Semen Fagopyri Esculenti) inhibitory action to Mycelium Growth of Ganoderma lucidum during liquid fermentation,
Simultaneously without liquid fermentation waste water and residue contamination；
(2) a kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food of the present invention, the utilization not being simple divides
Then compounding forming is carried out from the active component of method of purification extraction Rhizoma Dioscoreae and the active component of Ganoderma, but with Ganoderma as strain
By solid fermentation, directly eat after converting Rhizoma Dioscoreae, both improve effect of Rhizoma Dioscoreae active component, enhance again ganoderma active
Divide ganoderan and the amount of Ganodenic acid, industrialization large-scale production can be realized；
(3) a kind of Ganoderma of the present invention convert Rhizoma Dioscoreae produce functional food method, in its product saponin content 2～
20mg/g butt matter, ganoderma polyoses content 10～100mg/g butt matter, ganoderic acid content 1～20mg/g butt matter.There is regulation
The effects such as blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.The Ganodenic acid extracted from this product and Saponin, ganoderan
There is regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor function equally.
Accompanying drawing explanation
Fig. 1 is that a kind of preparation flow utilizing Ganoderma to convert the method that Rhizoma Dioscoreae produces functional food of the present invention shows
It is intended to.
Detailed description of the invention
The mensuration of described saponin content use spectrophotography (Tang Zhonghou, Shi Xinmin, Sun Jian, Li Hongmin, Zhang Aijun:
Total Saponins of Chinese Yam assay and Genotypic Difference thereof. Agriculture in Jiangxi journal 2011,23 (2): 50～52).
The mensuration of described ganoderic acid content uses ultraviolet spectrophotometry, and (Wang Xiangfei, Pan Xudong, Liu Wenxia, etc. Hemerocallis citrina Baroni Herba Orostachyos
The mensuration of the content of middle total triterpene composition. China National folk medicine, 2009,18 (10): 11).
Described ganoderma polyoses content deducts reducing sugar equal to total sugar, and the mensuration of total sugar uses sulfuric acid-phynol method (Zhaoyang
Nanmu, Chang Jidong. Phenol sulfuric acid procedure and indirect iodometric processes measure ganoderma polyoses content and compare [J]. edible fungi, 2007, (3): 58
～61.), content of reducing sugar measures employing 3,5-dinitrosalicylic acid system (Miller GL. Use of
dinitrosalicylic acid reagent for determination of reducing sugars.
Analytical Chemistry. 1959,1:426 ~ 428.).
Described Ganoderma converts the evaluation methodology of Rhizoma Dioscoreae production functional food regulation blood fat and uses high blood lipid model Mus to comment
Valency (Cao Min etc.: sanchi flower total saponine hypotensive effect is studied. the bright traditional Chinese medical science, and 2012,27 (7): 1314-1315).Concrete operations
For: after 40 mices adapt to feed 5d with normal feedstuff, take tail hematometry Triglycerides in Serum content, with triglyceride
Level is randomly divided into 4 groups: Normal group, hyperlipidemia matched group, low dosage experimental group, high dose experimental group.Normal control
Group mice feed normal feedstuff (12% casein, 60.98% corn starch, 15% sucrose, 7% Semen Maydis oil, 1% vitamin Icing Sugar, 4%
Mineral salt, 0.02% cod-liver oil)；(2% gallbladder is solid for 15.75% lard stearin, 7.79% sucrose for hyperlipidemia model control group fed high lipid food
Alcohol, 0.5% cholic acid, 73.96% normal feedstuff；Low dosage and high dose group mice feed per kilogram high lipid food and contain 10 grams and 20
Gram Ganoderma convert Rhizoma Dioscoreae functional food.Each group mice the most freely ingests and drinks water, animal housing's temperature 18～22 DEG C.Give continuously
After medicine surrounding, tail vein takes blood, analyzes serum triglyceride, T-CHOL, high density lipoprotein and low-density lipoprotein
Bai Hanliang
Described Ganoderma conversion Rhizoma Dioscoreae produces the evaluation methodology of functional food regulation blood fat and uses barbital and the left kidney of ligation to move
(yellow will is new: Herba Visci, the hypotensive activity of the Cortex Eucommiae and the reality of acute toxicity in the Hypertensive Rats evaluation of arteries and veins combined induction
Test research. research and development of natural products, 2003,15 (3): 245-248).Hypertension model Mus is divided into three groups of blanks
The Ganoderma that group, low dose group and high dose group feed normal feedstuff respectively, per kilogram contains 10 grams converts Rhizoma Dioscoreae functional food
Normal feedstuff and per kilogram contain 20 grams Ganoderma convert Rhizoma Dioscoreae functional food normal feedstuff.Successive administration measures for 14 days
Blood pressure before being administered and after administration.
Described Ganoderma converts the evaluation methodology of Rhizoma Dioscoreae production functional food regulation blood glucose and uses alloxan induction
Diabetic rat model is evaluated.Induction reference literature (the Effect of FeSO such as Zhicai Zhang of model mouse4 treatment
On glucose metabolism in diabetic rats. Biometals (2008) 21:685 691) carry out.High blood
Model mouse is divided into three groups of blank groups, low dose group and high dose group to feed normal feedstuff respectively to sugar, per kilogram contains 10 grams
The Ganoderma that Ganoderma converts the normal feedstuff of Rhizoma Dioscoreae functional food and per kilogram contains 20 grams convert Rhizoma Dioscoreae functional food
Normal feedstuff.Successive administration measures the blood glucose value before being administered and after administration for 28 days.
Described Ganoderma converts the Rhizoma Dioscoreae production radiation-resistant evaluation methodology of functional food and uses radiation irradiation in rats to carry out
Evaluate (a diplodocus man of virtue and ability. the effect study of Lac regis apis Antiradiation injury. Heilungkiang medical science, 2012,36 (10): 724-726).30
After the normal feedstuff 15 days that SD male rat feeds normal feedstuff respectively, convert Rhizoma Dioscoreae functional food containing 1% and 2% Ganoderma, greatly
Mus is feeding60Co gamma-rays carries out total irradiation, away from radiation source 1.0 m close rate 2.0 Gy, accumulated dose 200rad.After irradiating 10 days
Measuring body weight, mouse mortality rate, tail vein takes haemanalysis quantity of leucocyte.
Described Ganoderma converts Rhizoma Dioscoreae and produces the evaluation methodology employing suppression primary hepatocarcinoma of functional food suppression tumor
Cell Hepg2 growth effect (Zhouning County, Chen Jiangtao, Yu Wenyan. Water Extract of Ficus Carica to suppression tumor cell proliferation make
Preliminary study. Xinjiang Medicine University's journal. 2016,39 (1): 42-47).Hepg2 groups of cells complete culture solution is adjusted
Whole cell concentration is 2 × 104Individual/mL, is inoculated in 96 orifice plates, and every hole cumulative volume is 200 μ L, and overnight incubation makes cell attachment, secondary
Day converts the ethanol extract of Rhizoma Dioscoreae functional food and aqueous extract (substrate: water is (or anhydrous with the Ganoderma of various dose respectively
Ethanol)=1:10,70 DEG C are extracted 4 hours) and processing cell 24h respectively, matched group adds PBS and processes 24h, and each mensuration inoculates 3
Multiple hole, is placed in 37 DEG C, 5%CO2After incubator processes 24, every hole adds 5mg/mL tetrazolium bromide (MTT) reagent 20 μ L, continues to cultivate 4h,
Adding dimethyl sulfoxide (DMSO) 150 μ L, vibration mixing 10min again, (OD value is thin with alive to measure OD value by microplate reader (λ=490nm)
Born of the same parents' number is directly proportional), cell inhibitory rate=1-(dosing group OD value/matched group OD value), experiment is repeated 3 times.
Below in conjunction with specific embodiment, the present invention is further described below.
Embodiment 1
Utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps as it is shown in figure 1, a kind of:
(1) test tube amplification culture: Ganoderma (Ganoderma sinense) CGMCC 5.69 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 15 days for 20 DEG C, prepare test tube slant strain, this test tube
4 DEG C of inclined-plane saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 10 pieces
Being inoculated in the 250mL triangular flask equipped with 150mL liquid submerged culture base, triangular flask is 50 revs/min at rotating speed, temperature 35 DEG C
Under the conditions of, 18h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 10 under the conditions of 130 DEG C
Min prepares, and containing wheat bran 5g, the raw material of the Rhizoma Dioscoreae 20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 1% by volume, it is 35 DEG C in temperature, stirs
Mixing rotating speed is 160 revs/min, is the ventilation of 0.2:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 96h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 10 under the conditions of 130 DEG C
Min prepares, and containing wheat bran 5g, the raw material of the Rhizoma Dioscoreae 20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with RHIIZOMA DIOSCOREAE fr0m Henan of China (Dioscorea oppositaThunb) in solid fermentation culture medium, and described Ganoderma seed tank strain is with solid fermentation culture medium by weight
Being the inoculum concentration inoculation of 10%, temperature 20 DEG C, humidity 80% is fermented 40 days, and wherein every 6 days, stirring once, send out by prepared solid
Ferment thing；Described solid fermentation culture medium is that sterilizing 360 min prepares under the conditions of 130 DEG C, and solid medium is by every kilogram
The Rhizoma Dioscoreae of 10000 revs/min of homogenate adds the solid matrix raw materials such as 200g rice, 100g Semen Tritici aestivi, 100g Semen Maydis and 700g Sorghum vulgare Pers.
Prepare；Raw material in described solid fermentation culture medium mixes with water by weight the ratio for 1:1.1；
(5) by the solid fermentation thing obtained by step (4) after 120 DEG C dry 10 hours, it is crushed to below 100 mesh, packaging
Obtain edible functional food；Described functional food contains the Saponin of 2mg/g butt matter, 10mg/g butt matter
Ganoderan, the Ganodenic acid of 1mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-spoke
Penetrate and suppress the effects such as tumor；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 60% of 5 times of volumes of body weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid merit
Energy property food, this food Saponin and ganoderic acid content are respectively 25% and 70%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 5 times of volumes of mycelium weight
Water, 80 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.5 times
95% ethanol, under the conditions of 4 DEG C crystallize 5 hours, filter, filtrate continuously add extract raffinate volume 2.5 times 95% ethanol,
Crystallizing 5 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 20%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 2
A kind of utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Ganoderma lucidum (Leyss. Ex Fr.) Karst. (Ganoderma lucidum) CICC 14045 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 4 days for 35 DEG C, prepare test tube slant strain, and this test tube is oblique
4 DEG C of face saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 3 pieces
Being inoculated in the 250mL triangular flask equipped with 20mL liquid submerged culture base, triangular flask is 200 revs/min at rotating speed, temperature 20 DEG C
Under the conditions of, 86h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 60 under the conditions of 100 DEG C
Min prepares, and containing bran 20g, the raw material of the Rhizoma Dioscoreae 5g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 20% by volume, it is 20 DEG C in temperature, stirs
Mixing rotating speed is 50 revs/min, is 0.2～1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of ventilation, cultivate 18h, make Ganoderma seed tank strain；Described seed tank amplification culture base is to go out under the conditions of 100 DEG C
Bacterium 60min prepares, and containing wheat bran 20g, the raw material of the Rhizoma Dioscoreae 5g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with mesa medicine (Dioscorea alataL.) in solid fermentation culture medium, and described Ganoderma seed tank strain is 2% with solid fermentation culture medium by weight
Inoculum concentration is inoculated, and temperature 35 DEG C, humidity 80% is fermented 20 days, and wherein every 3 days, stirring once, prepares solid fermentation thing；Institute
Stating solid fermentation culture medium is that sterilizing 90min prepares under the conditions of 100 DEG C, and solid medium is by 10000 revs/min every kilogram
The Rhizoma Dioscoreae of homogenate adds the solid matrix raw materials such as 400g rice, 300g Semen Tritici aestivi, 300g Semen Maydis and 300g Sorghum vulgare Pers. and prepares；Described solid
Raw material in fermentation medium mixes with water by weight the ratio for 1:1.5；
(5) by the solid fermentation thing obtained by step (4) after 80 DEG C dry 10 hours, it is crushed to below 100 mesh, packs
To edible functional food；Described functional food contains the Saponin of 20mg/g butt matter, 100mg/g butt matter
Ganoderan, the Ganodenic acid of 20mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-
The effects such as radiation and suppression tumor；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 100% of 20 times of volumes of body weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid
Functional food, this food Saponin and ganoderic acid content are respectively 60% and 30%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 20 times of volumes of mycelium weight
Water, 100 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.5 times
95% ethanol, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extract raffinate volume 3.5 times 95%
Ethanol, crystallizes 18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 60%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 3
A kind of utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum) GIM5.282 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 10 days for 28 DEG C, prepare test tube slant strain, this test tube
4 DEG C of inclined-plane saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 7 pieces
Being inoculated in the 250mL triangular flask equipped with 80mL liquid submerged culture base, triangular flask is 120 revs/min at rotating speed, temperature 28 DEG C
Under the conditions of, 57h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 35 under the conditions of 120 DEG C
Min prepares, and containing wheat bran 13g, the raw material of the Rhizoma Dioscoreae 13g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 10% by volume, it is 28 DEG C in temperature, stirs
Mixing rotating speed is 110 revs/min, is the ventilation of 1:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of, cultivate 57h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 120 DEG C
35min prepares, and containing wheat bran 13g, the raw material of the Rhizoma Dioscoreae 13g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with sole sweet potato (Dioscorea alataL.f. flabella Makino.) in Rhizoma Dioscoreae solid fermentation culture medium, and described Ganoderma seed tank strain is sent out with solid
Ferment culture medium is the inoculum concentration inoculation of 6% by weight, and temperature 28 DEG C, humidity 80% is fermented 30 days, wherein every 4.5 days, turned over
Material once, prepares solid fermentation thing；Described solid fermentation culture medium is that sterilizing 260 min prepares under the conditions of 120 DEG C, and solid
Culture medium adds 300g rice, 200g Semen Tritici aestivi, 200g Semen Maydis and 500g Sorghum vulgare Pers. by the Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate
Prepare Deng solid matrix raw material；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:0.6；
(5) solid fermentation thing is after 100 DEG C dry 17 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 11mg/g butt matter, and the ganoderan of 54mg/g butt matter, 11mg/g are dry
The Ganodenic acid of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor
Effect；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 80% of 12 times of volumes of body weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 11 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid
Functional food, this food Saponin and ganoderic acid content are respectively 43% and 50%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 13 times of volumes of mycelium weight
Water, 90 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 12 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3 times, in 4
Crystallizing 12 hours under the conditions of DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 41%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 4
A kind of utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps:
(1) test tube amplification culture: Ganoderma (Ganoderma sinense) CGMCC 5.69 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 12 days for 23 DEG C, prepare test tube slant strain, this test tube
4 DEG C of inclined-plane saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 8 pieces
Being inoculated in the 250mL triangular flask equipped with 30mL liquid submerged culture base, triangular flask is 75 revs/min at rotating speed, temperature 20～35
Under conditions of DEG C, 30h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing under the conditions of 100 DEG C
55 min prepare, and containing wheat bran 8g, the raw material of the Rhizoma Dioscoreae 12g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 4% by volume, it is 25 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 0.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 35h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 55 under the conditions of 100 DEG C
Min prepares, and containing wheat bran 8g, the raw material of the Rhizoma Dioscoreae 15g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with dioscorea japonica (Dioscorea japonicaThunb.) in solid fermentation culture medium, and described Ganoderma seed tank strain is with solid fermentation culture medium by weight
Being the inoculum concentration inoculation of 4%, temperature 22 DEG C, humidity 80% is fermented 25 days, and wherein every 3 days, stirring once, prepares solid fermentation
Thing；Described solid fermentation culture medium is that sterilizing 100 min prepares under the conditions of 100 DEG C, and solid medium is by every kilogram 10000
The Rhizoma Dioscoreae of rev/min homogenate adds the solid matrix raw material such as 250g rice, 150g Semen Tritici aestivi, 150g Semen Maydis and 600g Sorghum vulgare Pers. and prepares；
Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:1.0；
(5) by the solid fermentation thing obtained by step (4) after 90 DEG C dry 22 hours, it is crushed to below 100 mesh, packs
To edible functional food；Described functional food contains the Saponin of 18mg/g butt matter, 90mg/g butt matter
Ganoderan, the Ganodenic acid of 5mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-spoke
Penetrate and suppress the effects such as tumor；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 70% of 7 times of volumes of body weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 6 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid merit
Energy property food, this food Saponin and ganoderic acid content are respectively 31% and 41%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 7 times of volumes of mycelium weight
Water, 85 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.7 times
95% ethanol, under the conditions of 4 DEG C crystallize 7 hours, filter, filtrate continuously add extract raffinate volume 2.8 times 95% ethanol,
Crystallizing 7 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 31%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 5
A kind of utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Ganoderma lucidum (Leyss. Ex Fr.) Karst. (Ganoderma lucidum) CICC 14045 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 6 days for 32 DEG C, prepare test tube slant strain, and this test tube is oblique
4 DEG C of face saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 40mL liquid submerged culture base, triangular flask is 160 revs/min at rotating speed, temperature 30 DEG C
Under the conditions of, 36h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 28 under the conditions of 128 DEG C
Min prepares, and containing wheat bran 6g, the raw material of the Rhizoma Dioscoreae 20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 30 DEG C in temperature, stirs
Mixing rotating speed is 160 revs/min, is the ventilation of 0.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 68h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 28 under the conditions of 128 DEG C
Min prepares, and containing wheat bran 15g, the raw material of the Rhizoma Dioscoreae 7g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with brown bud potato (Dioscorea persimilisPrain et Burk.) in Rhizoma Dioscoreae solid fermentation culture medium, and described Ganoderma seed tank strain and solid fermentation
Culture medium is the inoculum concentration inoculation of 6% by weight, and temperature 30 DEG C, humidity 80% is fermented 28 days, wherein every 5 days, and stirring one
Secondary, prepare solid fermentation thing；Described solid fermentation culture medium is that sterilizing 320 min prepares under the conditions of 128 DEG C, and solid culture
It is solid that base adds 350g rice, 250g Semen Tritici aestivi, 250g Semen Maydis and 400g Sorghum vulgare Pers. etc. by the Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate
Body base starting material prepares；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:0.6；
(5) solid fermentation thing is after 90 DEG C dry 18 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 4mg/g butt matter, and the ganoderan of 24mg/g butt matter, 16mg/g are dry
The Ganodenic acid of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor
Effect；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 90% of 17 times of volumes of body weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 15 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid
Functional food, this food Saponin and ganoderic acid content are respectively 25% and 61%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 18 times of volumes of mycelium weight
Water, 95 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.4 times
95% ethanol, under the conditions of 4 DEG C crystallize 16 hours, filter, filtrate continuously add extract raffinate volume 3.2 times 95% second
Alcohol, crystallizes 16 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 52%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 6
A kind of utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum) GIM5.282 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 5 days for 32 DEG C, prepare test tube slant strain, and this test tube is oblique
4 DEG C of face saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 20mL liquid submerged culture base, triangular flask is 170 revs/min at rotating speed, temperature 25 DEG C
Under the conditions of, 60h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 50 under the conditions of 110 DEG C
Min prepares, and containing wheat bran 16g, the raw material of the Rhizoma Dioscoreae 8g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 25 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 1.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 29h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 50 under the conditions of 110 DEG C
Min prepares, and containing wheat bran 14g, the raw material of the Rhizoma Dioscoreae 10g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with spit of fland Rhizoma Dioscoreae (Dioscorea quinquelabaThunb.) in solid fermentation culture medium, and described Ganoderma seed tank strain presses weight with solid fermentation culture medium
Amount is inoculated than the inoculum concentration being 6%, and temperature 25 DEG C, humidity 80% is fermented 26 days, and wherein every 5 days, stirring once, prepares solid
Fermented product；Described solid fermentation culture medium is that sterilizing 250 min prepares under the conditions of 110 DEG C, and solid medium is by every kilogram
The Rhizoma Dioscoreae of 10000 revs/min of homogenate adds the solid matrix raw materials such as 400g rice, 150g Semen Tritici aestivi, 150g Semen Maydis and 500g Sorghum vulgare Pers.
Prepare；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:0.8；
(5) by the solid fermentation thing obtained by step (4) after 110 DEG C dry 16 hours, it is crushed to below 100 mesh, packaging
Obtain edible functional food；Described functional food contains the Saponin of 6mg/g butt matter, 56mg/g butt matter
Ganoderan, the Ganodenic acid of 18mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-spoke
Penetrate and suppress the effects such as tumor；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 63% of 11 times of volumes of body weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 12 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid
Functional food, this food Saponin and ganoderic acid content are respectively 55% and 37%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 13 times of volumes of mycelium weight
Water, 90 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 13 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3.0 times,
Crystallizing 13 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 45%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 7
A kind of utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps:
(1) test tube amplification culture: Ganoderma (Ganoderma sinense) CGMCC 5.69 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 13 days for 23 DEG C, prepare test tube slant strain, this test tube
4 DEG C of inclined-plane saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 10 pieces
Being inoculated in the 250mL triangular flask equipped with 130mL liquid submerged culture base, triangular flask is 150 revs/min at rotating speed, temperature 23 DEG C
Under conditions of, 36h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 53 under the conditions of 115 DEG C
Min prepares, and containing wheat bran 9g, the raw material of the Rhizoma Dioscoreae 20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 21 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 0.2:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 81h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 53 under the conditions of 115 DEG C
Min prepares, and containing wheat bran 19g, the raw material of the Rhizoma Dioscoreae 18g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with Rhizoma Dioscoreae (Dioscorea oppositaFoshou) in solid fermentation culture medium, and described Ganoderma seed tank strain is with solid fermentation culture medium by weight
Being the inoculum concentration inoculation of 4%, temperature 21 DEG C, humidity 80% is fermented 34 days, and wherein every 6 days, stirring once, prepares solid fermentation
Thing；Described solid fermentation culture medium is that sterilizing 250 min prepares under the conditions of 110 DEG C, and solid medium is by every kilogram 10000
The Rhizoma Dioscoreae of rev/min homogenate adds the solid matrix raw material such as 400g rice, 150g Semen Tritici aestivi, 250g Semen Maydis and 400g Sorghum vulgare Pers. and prepares；
Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:1.0；
(5) solid fermentation thing is after 115 DEG C dry 16 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 20mg/g butt matter, the ganoderan of 100mg/g butt matter, 1mg/g
The Ganodenic acid of butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc.
Effect；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 75% of 9 times of volumes of body weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 10 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid
Functional food, this food Saponin and ganoderic acid content are respectively 38% and 45%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 10 times of volumes of mycelium weight
Water, 88 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.8 times
95% ethanol, under the conditions of 4 DEG C crystallize 10 hours, filter, filtrate continuously add extract raffinate volume 2.9 times 95% second
Alcohol, crystallizes 10 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 38%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 8
A kind of utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Ganoderma lucidum (Leyss. Ex Fr.) Karst. (Ganoderma lucidum) CICC 14045 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 10 days for 29 DEG C, prepare test tube slant strain, this test tube
4 DEG C of inclined-plane saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 8 pieces
Being inoculated in the 250mL triangular flask equipped with 60mL liquid submerged culture base, triangular flask is 160 revs/min at rotating speed, temperature 33 DEG C
Under the conditions of, 80h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 30 under the conditions of 120 DEG C
Min prepares, and containing wheat bran 19g, the raw material of the Rhizoma Dioscoreae 19g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 18% by volume, it is 33 DEG C in temperature, stirs
Mixing rotating speed is 140 revs/min, is the ventilation of 1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 90h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 30 under the conditions of 120 DEG C
Min prepares, and containing wheat bran 18g, the raw material of the Rhizoma Dioscoreae 18g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with Fructus Citri Sarcodactylis Rhizoma Dioscoreae (Dioscorea oppositaIron-rod) in solid fermentation culture medium, and described Ganoderma seed tank strain is with solid fermentation culture medium by weight
Inoculating than the inoculum concentration being 9%, temperature 33 DEG C, humidity 80% is fermented 37 days, and wherein every 6 days, stirring once, send out by prepared solid
Ferment thing；Described solid fermentation culture medium is that sterilizing 350 min prepares under the conditions of 105 DEG C, and solid medium is by every kilogram
The Rhizoma Dioscoreae of 10000 revs/min of homogenate adds the solid matrix raw materials such as 280g rice, 220g Semen Tritici aestivi, 270g Semen Maydis and 380g Sorghum vulgare Pers.
Prepare；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:1.4；
(5) solid fermentation thing is after 85 DEG C dry 23 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 18mg/g butt matter, and the ganoderan of 94mg/g butt matter, 3mg/g are dry
The Ganodenic acid of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor
Effect；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 97% of 16 times of volumes of body weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 15 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid
Functional food, this food Saponin and ganoderic acid content are respectively 26% and 67%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 18 times of volumes of mycelium weight
Water, 95 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.4 times
95% ethanol, under the conditions of 4 DEG C crystallize 16 hours, filter, filtrate continuously add extract raffinate volume 3.2 times 95% second
Alcohol, crystallizes 6 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 57%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Embodiment 9
A kind of utilize Ganoderma to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum) GIM5.282 test tube slant strain is cut into 3 × 3
Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 6 days for 24 DEG C, prepare test tube slant strain, and this test tube is oblique
4 DEG C of face saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 24mL liquid submerged culture base, triangular flask is 58 revs/min at rotating speed, temperature 24 DEG C
Under the conditions of, 20h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 59 under the conditions of 100 DEG C
Min prepares, and containing wheat bran 5g, the raw material of the Rhizoma Dioscoreae 5g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 3% by volume, it is 24 DEG C in temperature, stirs
Mixing rotating speed is 58 revs/min, is the ventilation of 1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of, cultivate 25h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 59 under the conditions of 100 DEG C
Min prepares, and containing wheat bran 5g, the raw material of the Rhizoma Dioscoreae 6g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Ganoderma seed tank strain access equipped with mountain potato (Dioscorea fordii Prain et Burk.) in Rhizoma Dioscoreae solid fermentation culture medium, and described Ganoderma seed tank strain is cultivated with solid fermentation
Base is the inoculum concentration inoculation of 3% by weight, and temperature 24 DEG C, humidity 80% is fermented 23 days, and wherein every 3 days, stirring once, is made
Obtain solid fermentation thing；Described solid fermentation culture medium is that sterilizing 150 min prepares under the conditions of 100 DEG C, and solid medium is pressed
The Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate adds the solid-based such as 360g rice, 240g Semen Tritici aestivi, 160g Semen Maydis and 540g Sorghum vulgare Pers.
Raw material prepares；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:1.3；
(5) solid fermentation thing is after 110 DEG C dry 13 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 2mg/g butt matter, and the ganoderan of 10mg/g butt matter, 20mg/g are dry
The Ganodenic acid of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor
Effect；
(6) separation is extracted: the Ganoderma obtained by step (4) or (5) is converted the functional food of Rhizoma Dioscoreae production respectively through carrying
Take, respectively obtain containing Saponin and the product of Ganodenic acid and the product of ganoderan.
It should be noted that and comprise the steps: from the method for the product containing Saponin and Ganodenic acid described in step (6)
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
The ethanol of the 81% of 15 times of volumes of body weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 14 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganodenic acid
Functional food, this food Saponin and ganoderic acid content are respectively 57% and 36%.
In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 13 times of volumes of mycelium weight
Water, 92 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 12 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3.1 times,
Crystallizing 12 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 42%；
Animal experiment proves, Saponin and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood
The effects such as sugar and blood pressure, radioprotective and suppression tumor.
Schematically being described the present invention and embodiment thereof above, this description does not has restricted, actual knot
Structure is not limited thereto.So, if those of ordinary skill in the art is enlightened by it, without departing from the invention objective
In the case of, design the frame mode similar to this technical scheme and embodiment without creative, all should belong to the present invention's
Protection domain.
Claims (10)
1. one kind utilizes Ganoderma to convert the method that Rhizoma Dioscoreae produces functional food, it is characterised in that described preparation method is with Rhizoma Dioscoreae
It is solid matrix with rice, Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers. etc., with Ganoderma as starting strain, sequentially passes through test tube amplification culture, liquid
Shake-flask culture and seed tank amplification culture, solid fermentation cultivate, dry, pulverize and prepared by the technique such as packaging；Described is functional
Food contains the Saponin of 2～20mg/g butt matter, the ganoderan of 10～100mg/g butt matter, 1～the spirit of 20mg/g butt matter
Sesame acid, has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor；Described functional food can extract
Its active component Saponin, ganoderan and Ganodenic acid, be used for producing treatment and reduce blood fat, blood glucose and blood pressure, radioprotective and suppression
Medicine or the functional foods such as the tablet of tumor or capsule.
A kind of Ganoderma the most according to claim 1 convert Rhizoma Dioscoreae produce functional food method, it is characterised in that institute
The Rhizoma Dioscoreae raw material used includes: RHIIZOMA DIOSCOREAE fr0m Henan of China (Dioscorea oppositaThunb), mesa medicine (Dioscorea alata
L.) and mutation sole sweet potato (Dioscorea alataL.f. flabella Makino.) and include dioscorea japonica
(Dioscorea japonicaThunb.), brown bud potato (Dioscorea persimilisPrain et Burk.), mountain potato
(Dioscorea fordii Prain et Burk.) and spit of fland Rhizoma Dioscoreae (Dioscorea quinquelabaThe Tu Shan such as Thunb.)
Medicine, Rhizoma Dioscoreae (Dioscorea oppositaFoshou) or Fructus Citri Sarcodactylis Rhizoma Dioscoreae (Dioscorea opposita Iron-
rod)；The Ganderma lucidum strain used include Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum), Ganoderma (Ganoderma lucidum) or
Ganoderma (Ganoderma sinensis)。
A kind of Ganoderma the most according to claim 1 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that described
Preparation method comprises the steps:
A1 test tube amplification culture: Ganoderma slant strains be inoculated in potato dextrose medium and cultivate, prepares Ganoderma
Test tube slant strain；
A2 liquid submerged culture: the Ganoderma test tube slant strain obtained by step A1 is inoculated into equipped with liquid submerged culture base
In shaking flask, cultivate, prepare ganoderma lucidum liquid shaking flask strain；
A3 seed tank amplification culture: the ganoderma lucidum liquid shaking flask strain obtained by step A2 is inoculated in seed tank culture base and carries out
Cultivate, make Ganoderma seed tank strain；
A4 solid fermentation is cultivated: be inoculated in solid medium by the Ganoderma seed tank strain obtained by step A3, and mixes all
Even, carry out fermentation culture, prepare Ganoderma solid fermentation thing；
A5 packs: Ganoderma solid fermentation thing converts the functional food of Rhizoma Dioscoreae through drying, pulverize, pack prepared Ganoderma.
A kind of Ganoderma the most according to claim 3 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that described
Preparation method is specific as follows:
B1 test tube amplification culture: slant strains in Ganoderma test tube is cut into 3 × 3 mm fritter strains, inoculates a fritter oblique to test tube
In the culture medium of face, cultivating 4～15 days for 20～35 DEG C, prepare test tube slant strain, 4 DEG C, this test tube slant saves backup；
B2 liquid submerged culture: the test tube slant strain obtained by step B1 is cut into 3 × 3 mm fritter strains, picking 3～10
Block is inoculated in the 250mL triangular flask equipped with 20～150mL liquid submerged culture bases, triangular flask rotating speed be 50～200 turns/
Point, under conditions of temperature 20～35 DEG C, 18-86h cultivated by shaking table, makes liquid shaking bottle strain；
B3 seed tank amplification culture: the liquid shaking bottle strain obtained by step B2 is inoculated in seed tank amplification culture base, and
Described liquid shaking bottle strain and seed tank amplification culture base are the inoculum concentration inoculation of 1～20% by volume, are 20～35 in temperature
DEG C, speed of agitator is 50～160 revs/min, is 0.2 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
～under the conditions of the ventilation of 1.8:1, cultivate 18～96h, make Ganoderma seed tank strain；
B4 solid fermentation is cultivated: accessed in solid fermentation culture medium by the Ganoderma seed tank strain obtained by step B3, and described
Ganoderma seed tank strain and solid fermentation culture medium are the inoculum concentration inoculation of 2～10% by weight, temperature 20～35 DEG C, wet
Degree 80% fermentation 5～20 days, wherein every 3～6 days, stirring once, prepares solid fermentation thing；
B5 solid fermentation thing, after 80～120 DEG C dry 10～24 hours, is crushed to below 100 mesh, is packaged to be and can eat
Functional food.
A kind of Ganoderma the most according to claim 4 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that step
Liquid submerged culture base described in B2 is that sterilizing 10～60 min prepares under the conditions of 100～130 DEG C, and every liter of liquid shaking bottle training
Supporting containing wheat bran 5～20g, the raw material of the Rhizoma Dioscoreae 5～20g of 10000 revs/min of homogenate in base, seed tank described in step B3 expands
Big culture medium is that sterilizing 10～60 min prepares under the conditions of 100～130 DEG C, and containing wheat bran in every liter of liquid submerged culture base
5～20g, the raw material of the Rhizoma Dioscoreae 5～20g of 10000 revs/min of homogenate.
A kind of Ganoderma the most according to claim 4 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that step
Solid fermentation culture medium described in B4 is that sterilizing 90～360 min prepares under the conditions of 100～130 DEG C, and every kilogram 10000
The Rhizoma Dioscoreae of rev/min homogenate adds 200～400g rice, 100～300g Semen Tritici aestivi, 100～300g Semen Maydis and 700～300g Sorghum vulgare Pers.
Deng solid matrix raw material.
A kind of Ganoderma the most according to claim 7 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that described
Solid matrix in solid fermentation culture medium mixes with water by weight the ratio of 1:0.6～1.5.
A kind of Ganoderma the most according to claim 1 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that step
Convert, from Ganoderma, Saponin and the method for Ganodenic acid extracted the functional food that Rhizoma Dioscoreae produces described in A4, A5 or step B4, B5
Comprise the steps:
Obtained Ganoderma is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds mycelia
60～the ethanol of 100% of 5～20 times of volumes of body weight, repeatedly extracts 3～5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3～18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and Ganoderma
Acid function food, this food Saponin and ganoderic acid content are respectively 25～60% and 30～70%.
A kind of Ganoderma the most according to claim 1 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that step
Convert from Ganoderma described in A4, A5 or step B4, B5 and the functional food that Rhizoma Dioscoreae produces extracts the method for ganoderan include
Following steps:
Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 5～20 times of volumes of mycelium weight
Distilled water, 80～100 DEG C are repeatedly extracted 3～5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, add residual volume 0.5～
95% ethanol of 1.5 times, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extraction raffinate volume 2.5～
95% ethanol of 3.5 times, crystallizes 5～18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan
Purity 20～60%.
10. convert the method that Rhizoma Dioscoreae produces functional food, step C2 and D2 processing according to a kind of Ganoderma described in right 1
The ganoderan obtained or Ganodenic acid and Saponin, can be processed into tablet and capsule according to conventional tablet or capsule preparation method,
The tablet being processed into and capsule have regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
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| CN109259199A (en) * | 2018-10-16 | 2019-01-25 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of coprinus comatus and barley |
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| CN107212333A (en) * | 2017-07-10 | 2017-09-29 | 张志才 | The fermentate of the golden solid state transformed Chinese yam of ear and its application |
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| CN109259198A (en) * | 2018-10-16 | 2019-01-25 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of cicada fungus bacterium and bean cake powder |
| CN109393444A (en) * | 2018-10-16 | 2019-03-01 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed ginger of coprinus comatus and barley |
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Application publication date: 20161207 |