CN1302902A - Process for preparing activated polyose by biologic technique and its product - Google Patents
Process for preparing activated polyose by biologic technique and its product Download PDFInfo
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- CN1302902A CN1302902A CN 01106815 CN01106815A CN1302902A CN 1302902 A CN1302902 A CN 1302902A CN 01106815 CN01106815 CN 01106815 CN 01106815 A CN01106815 A CN 01106815A CN 1302902 A CN1302902 A CN 1302902A
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Abstract
A process for biologically preparing active polyose uses the Chinese medicine as culture medium, and includes such steps as inoculating the composite bacteria including ganoderma, yeast and cordyceps, culturing at 15-28 deg.C for 17-25 days, biologic fermenting until the fungus polyose reaches the first peak, stopping fermentation, dewatering, biochemically extracting active polyose or drying and pulverizing, and finally sterilizing. Its product can increase human immunity for preventing and treating cancers without toxic by-effect.
Description
The present invention relates to a kind of biotechnology, refer to especially carry out biological fermentation, produce the method for fungus polysaccharide with herbal medicine and fungi.The invention still further relates to the product of producing with this method.
Studies have shown that in a large number that both at home and abroad medicinal fungus such as Chinese caterpillar fungus, glossy ganoderma have significant inhibition lethal effect to cancer cells.Recent studies proves that Cordyceps polysaccharide, cordycepin are good biological regulator, antimutagens, can strengthen immune function of human body, improves immunocompetence.Its antitumour activity is the combination rate that improves normal people and leukemia NK cells of human beings activity and K562 cell, and the direct killing tumour cell stops cancer cells to increase.In addition, also have reducing blood lipid, hemopoietic system green blood improves oxyphorase and hemochrome; Also can correct myocardial ischemia, improve effects such as coronary artery circulation, treatment arrhythmia, anticoagulant, antithrombotic formation.Ganoderma lucidum seu Japonicum, the effect of liver-kidney tonifying is arranged, promoting longevity.Ganoderan can suppress tumour, mediator's body immunity function, two-ways regulation blood pressure; promote the human endocrine balance, the hemopoietic system nourishes blood, and the protection liver cell avoids poisoning and damages; suppress thrombosis, disintegrate effects such as on every side thrombus of tissue, blood fat reducing, blood viscosity lowering.
Medicinal fungus such as Chinese caterpillar fungus, glossy ganoderma resource is few, adds that people are unreasonable to excavate medicine source deficiency.Therefore cost an arm and a leg, be difficult to the people of wage-earners and utilize.People when illnesss such as treatment diabetes, cardiovascular diseases, conventional Chinese medicine.Though some curative effects are arranged, because some macromolecular substance of Chinese medicine fail to degrade, people absorb few, and drug effect fails to give full play to, so curative effect is limited.Therefore, in bio-science growing today, people have carried out a large amount of research to artificial propagation and the processing and utilization of Chinese caterpillar fungus, glossy ganoderma.The patent application of relevant this respect reaches tens of.For example application number is 93110814 " beverage made of natural edible and medicinal mushroom ", utilizes edible medicinal fungus do fermentation body spontaneous fermentation breedings in dark basic culture medium solution such as glossy ganoderma.Application number is 89104005 " continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix ".Application number is 96103521 " Chinese medicine nutrition oral administration ", is prepared from addition of Chinese yam, Chinese holly reading notes, Spina Date Seed and Radix Glycyrrhizae with glossy ganoderma, Hericium erinaceus (Bull. Ex Fr.) Pers. etc." product of edible mushrooms and herbal medicine mixed fermentation brewing and the method " of application number 93104737, adopting edible mushrooms and the effective prescription of a certain traditional Chinese medicine for oral administration is raw material, makes bacterial classification with the edible root certain kind of berries, through the multiple operation fermented soy, produce the ethanol of 3-5%, get mash and make commodity.
The objective of the invention is: solve the technical barrier of Chinese caterpillar fungus, glossy ganoderma artificial propagation, provide batch production to produce the technology of fungus polysaccharide and the Chinese medicinal materials macromolecular cpd is degraded and improve the technology of drug effect.
Another object of the present invention is: fungus polysaccharide goods and fungus polysaccharide and herbal medicine composite product are provided, produce the health care pharmaceuticals that existing high-grade nutrient health care effect has certain targeted therapy effect again.
The object of the present invention is achieved like this, and production technology is to be raw material with the effective prescription of herbal medicine for oral administration, and raw material is behind pre-treatment, sterilization, make substratum, fungi strains such as Chinese caterpillar fungus, glossy ganoderma are mixed the back inoculation, the solid biologic fermentation, culture temperature was cultivated 17-25 days down for 15～28 ℃.When reaching first peak value, fungus polysaccharide content stops cultivating, dehydration then, and biochemical extraction active polysaccharide, or dehydrate, pulverize, quality inspection again, sterilization, packing.
Its product is according to different the having any different of composite bacteria of composition of raw materials and inoculation.
The present invention one has solved Chinese caterpillar fungus, the technology of glossy ganoderma artificial propagation and the technology that fungus polysaccharide is produced in batch production; The 2nd, adopt solid biologic fermentation and composite bacteria inoculation, make the macromolecular cpd degraded of herbal medicine, be beneficial to absorption, improve drug effect.For entering the ordinary people man, precious medicines such as Chinese caterpillar fungus opened up road, for people provide the high-class healthy product.
Concrete grammar of the present invention is as follows: accompanying drawing is seen in technical process, and is as follows below in conjunction with description of drawings.
First operation: with herbal prescription 1, with Chinese medicine material pre-treatment 2, promptly rogue, be ground into 30～50 orders after the impurity elimination, dehydration, add water 50～150% by the raw material gross weight again, pH value 5.5～8, bottling or pack are under 0.8～1.5kg/cm2 sterilization 1-4 hour (in the accompanying drawing 3), cooling promptly gets substratum.
Second operation: preparation composite bacteria 4, composite bacteria is mixed and is formed by yeast, glossy ganoderma, Chinese caterpillar fungus, mushroom fungi, and the consumption of each bacterial classification can be similar and different.Inoculate composite bacteria with the substratum that first operation makes under aseptic condition, inoculum size is the 1-5% of substratum.Inoculation back solid biologic fermentation 5, promptly controlled temperature was cultivated 17～25 days down at 15～28 ℃, stopped cultivating when fungus polysaccharide content reaches first peak value.
The 3rd operation: dehydration, biochemical extraction fungus polysaccharide or grinding and processing become product.Raw material behind the biological fermentation is being reached dehydration below 70 ℃, and water content is controlled at below 15%, then according to the difference of raw material, can take way, quality inspection sterilization 10, the packing 11 of biochemical extraction 8 or dry (expanded) pulverizing 9 promptly to get this finished product 12 respectively.
Embodiment 1, the Worm grass and glossy ganoderma polysaccharides preparation method
With following 11 kinds of Chinese prescriptions, raw material weight ratio be:
Chinese caterpillar fungus, sealwort, Chinese yam, the bighead atractylodes rhizome, Glossy Privet Fruit, each 2-5 part of the red sage root, wolfberry fruit, shizandra berry, Herba Epimedii, each 1-4 part of Herba Epimedii, coptis 1-3 part.Rogue, impurity elimination, dehydration pulverize, and adds water 100% by the raw material gross weight again, pH value 5.5～6.5, bottling or pack, sterilization is at 1.0～1.5kg/cm
23 hours, the cooling back was at aseptic indoor inoculation composite bacteria.Composite bacteria is made up of yeast, glossy ganoderma, three kinds of mixing of mushroom, and the consumption of each bacterial classification equates.Cultivated 20～25 days at 20～28 ℃ of following alternating temperatures the inoculation back, stops cultivating the biochemical then active polysaccharide that extracts of dehydration (water content≤10%) when fungus polysaccharide content reaches first peak value.Last quality inspection, ultraviolet disinfection pack or go into capsule.The preferable weight ratio of each Chinese medicine material of this product is: respectively 4 parts of Chinese caterpillar fungus, sealwort, Chinese yam, the bighead atractylodes rhizome, Glossy Privet Fruit, the reds sage root, wolfberry fruit, shizandra berry, Herba Epimedii, each 2.5 parts of Herba Epimediis, 1.5 parts of the coptiss.It is respond well that this product is used for diabetes, is again first-class healthcare products.Share with other products, of many uses.
Embodiment 2 Chinese caterpillar funguses, glossy ganoderma body-building electuary
With following several Chinese prescriptions, each raw material weight ratio be:
Mushroom, black Semen Maydis, each 3-10 part of bitter buckwheat, Poria cocos, matrimony vine, each 0.5-3 part of rheum officinale, red sage root 0.4-8 part, root of kudzu vine 0.3-3 part, tuber of multiflower knotweed 0.3-3 part, pearl 0.3-2 part, black soya bean 2-8 part, gynostemma pentaphylla 0.8-8 part.
Raw material is rogued, impurity elimination, dehydration are pulverized, and adds water 120% by the raw material gross weight then, and pH value 5～7 is at 1.2kg/cm
2Under sterilized cooling back inoculation composite bacteria 3.5 hours.Composite bacteria is made up of yeast, glossy ganoderma, Chinese caterpillar fungus.15～28 ℃ of culture temperature were cultivated 17～25 days, stop to cultivate when fungus polysaccharide content reaches first peak value, dehydrated in 70 ℃, and water content reduces to 12% when following, extruding puffing, pulverizing, ultraviolet disinfection, quality inspection again, the packing of weighing.This product Chinese medicine material weight preferred proportion is: respectively 7 parts of mushroom, black Semen Maydis, bitter buckwheats, respectively 2 parts of Poria cocos, matrimony vine, rheum officinales, 6 parts of the reds sage root, 2 parts of the roots of kudzu vine, 2.5 parts of the tubers of multiflower knotweed, 1.5 parts of pearls, 6 parts in black soya bean, 7.5 parts of gynostemma pentaphylla.This product mixes with Chinese caterpillar fungus, ganoderan to be taken, good to the result of treatment of cardiovascular disorder.
The preparation of embodiment 3 Ganoderma teas
With the aforesaid method difference.The one, Chinese medicine preparation, its raw material weight ratio is: Ginkgo Leaf, gynostemma pentaphylla, each 4-8 part of tealeaves, glossy ganoderma, pearl, mushroom, each 1-5 part of the red sage root.The 2nd, composite bacteria is made up of yeast, Cordyceps.The 3rd, meal or fine powder granulation when pulverizing.This product mixes with Cordyceps polysaccharide to be taken, and treatment hepatitis B curative effect is obvious.Each Chinese medicine weight preferred proportion of this product is: respectively 5 parts of Ginkgo Leaf, gynostemma pentaphylla, tealeaves, respectively 2 parts of glossy ganoderma, pearl, mushroom, the reds sage root.
Embodiment 4, glossy ganoderma peptic or capsule, and its Chinese medicine preparation is: glutinous rice 1-3 part, dried orange peel 1-3 part, pearl 1-2 part, method summer 1-2 part, glossy ganoderma 2-4 part, coptis 0.5-1 part.Composite bacteria is made up of yeast, hedgehog hydnum bacterial classification.
The present invention with medication, dietotherapy is molten is integrated. Its product is the natural product of " harmony between man and nature ". Its feature Be: 1. have no side effect; 2. improve the immunity of human body, its antibody cell number in serum hemolysin and the spleen that can raise, Improve serum immunoglobulin level, can activate the phagocytic function of reticuloendothelial system, activating macrophage promotes TNK With the generation of IL-2, impel human organ function normalization. 3. not only mend but also control, strengthening vital QI to eliminate pathogenic factors is fundamentally treated disease Disease, in the recent period, late result is desirable, clinical being used for the treatment of " hypertension " " coronary heart disease " " diabetes " " hepatitis B " " disease of digestive system " etc. is efficient up to more than 95%, and clinical cure rate is greater than 60%, and is evident in efficacy, and it is a kind of Good medicine also is a kind of health products.
Claims (10)
1. with the method for preparing activated polyose by biologic technique, it is characterized in that: be that raw material is made substratum with Chinese medicine, inoculation glossy ganoderma, yeast composite bacteria carry out biological fermentation, and its processing step is:
A. the Chinese medicine of selecting for use is rogued, impurity elimination, take by weighing each Chinese medicine by formulation weight part, be ground into 20～40 orders after the dehydration, add water 50～150% by the raw material gross weight again, pH value 5.5～7, the bottling or the pack, at 0.8～1.5kg/cm
2Pressure was sterilized 1～4 hour down, then naturally cooling;
B. prepare composite bacteria, composite bacteria is mixed by 2～4 kinds of fungies, and the consumption of each bacterial classification can equate or not wait; Composite bacteria is being inoculated under the aseptic condition on the Chinese medicine material substratum; 15～28 ℃ of temperature bottom fermentations 17～25 days, when reaching first peak value, fungus polysaccharide stops cultivation and fermentation then;
C. dehydration, biochemical fungus polysaccharide or the grinding and processing extracted, in dehydration below 50～70 ℃, water content is controlled at 10～15% with the raw material behind the biological fermentation, then with prior art biochemistry extraction or drying and crushing, quality inspection, sterilization, pack product.
2. according to the method for claim 1, it is characterized in that: composite bacteria is yeast, glossy ganoderma, mushroom composition.
3. according to the method for claim 1, it is characterized in that: composite bacteria is yeast, glossy ganoderma, Chinese caterpillar fungus composition.
4. according to the method for claim 1 or 2, it is characterized in that each raw material weight ratio of Chinese medicine is: Chinese caterpillar fungus, sealwort, Chinese yam, the bighead atractylodes rhizome, Glossy Privet Fruit, each 2-5 part of the red sage root, wolfberry fruit, shizandra berry, Herba Epimedii, each 1-4 part of Herba Epimedii, coptis 1-3 part.
5. method according to claim 3, it is characterized in that: each raw material weight ratio of Chinese medicine is: mushroom, black Semen Maydis, each 3-10 part of bitter buckwheat, Poria cocos, matrimony vine, each 0.5-3 part of rheum officinale, red sage root 0.4-8 part, root of kudzu vine 0.3-3 part, tuber of multiflower knotweed 0.3-3 part, pearl 0.3-2 part, black soya bean 2-8 part, gynostemma pentaphylla 0.8-8 part.
6. method according to claim 1 and 2 is characterized in that: each raw material weight ratio of Chinese medicine is: Ginkgo Leaf, gynostemma pentaphylla, each 4-8 part of tealeaves, glossy ganoderma, pearl, mushroom, each 1-5 part of the red sage root.
7. the product of producing according to the method for claim 1.
8. according to the method for claim 1, select the product of the Chinese medicine production of claim 4 for use.
9. according to the method for claim 1, select the product of the Chinese medicine production of claim 5 for use.
10. according to the method for claim 1, select the product of the Chinese medicine production of claim 6 for use.
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Cited By (12)
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| CN1307314C (en) * | 2004-04-28 | 2007-03-28 | 中国食品发酵工业研究院 | Enzymolytic preparation method for glossy ganoderma amylose |
| CN1308350C (en) * | 2004-07-28 | 2007-04-04 | 武汉大学 | Process for preparing tuckahoe mycelium extracellular polysacchride with anti-tumor activity |
| CN1317391C (en) * | 2004-07-01 | 2007-05-23 | 阎波 | Method for extracting ganoderan by solid fermentation |
| CN102078016A (en) * | 2010-11-25 | 2011-06-01 | 怀宝城 | Glossy ganoderma and Chinese yam beverage and preparation method thereof |
| CN102599481A (en) * | 2012-03-12 | 2012-07-25 | 杨毅 | Method for preparing solid fermentation mixed edible medicinal fungus sporocarp |
| CN103183740A (en) * | 2013-03-22 | 2013-07-03 | 李士刚 | Production method of fructus schisandrae polysaccharide |
| CN104055184A (en) * | 2014-06-27 | 2014-09-24 | 杭州雪域生物技术有限公司 | Hirsutella sinensis hypolipidemic healthcare beverage and preparation method thereof |
| CN106174221A (en) * | 2016-07-12 | 2016-12-07 | 江苏大学 | A kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food |
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- 2001-01-03 CN CNB011068159A patent/CN1142283C/en not_active Expired - Fee Related
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| CN1307314C (en) * | 2004-04-28 | 2007-03-28 | 中国食品发酵工业研究院 | Enzymolytic preparation method for glossy ganoderma amylose |
| CN1317391C (en) * | 2004-07-01 | 2007-05-23 | 阎波 | Method for extracting ganoderan by solid fermentation |
| CN1308350C (en) * | 2004-07-28 | 2007-04-04 | 武汉大学 | Process for preparing tuckahoe mycelium extracellular polysacchride with anti-tumor activity |
| CN102078016A (en) * | 2010-11-25 | 2011-06-01 | 怀宝城 | Glossy ganoderma and Chinese yam beverage and preparation method thereof |
| CN102599481A (en) * | 2012-03-12 | 2012-07-25 | 杨毅 | Method for preparing solid fermentation mixed edible medicinal fungus sporocarp |
| CN103183740B (en) * | 2013-03-22 | 2015-06-10 | 李士刚 | Production method of fructus schisandrae polysaccharide |
| CN103183740A (en) * | 2013-03-22 | 2013-07-03 | 李士刚 | Production method of fructus schisandrae polysaccharide |
| CN104055184B (en) * | 2014-06-27 | 2015-07-08 | 杭州雪域生物技术有限公司 | Hirsutella sinensis hypolipidemic healthcare beverage and preparation method thereof |
| CN104055184A (en) * | 2014-06-27 | 2014-09-24 | 杭州雪域生物技术有限公司 | Hirsutella sinensis hypolipidemic healthcare beverage and preparation method thereof |
| WO2017186144A1 (en) * | 2016-04-29 | 2017-11-02 | 刘东波 | Microbial fermentation of botanicals |
| JP2019514424A (en) * | 2016-04-29 | 2019-06-06 | 長沙組学生物科技有限公司Ｃｈａｎｇｓｈａ Ｏｍｉｃｓ Ｂｉｏ−Ｔｅｃｈ Ｃｏ． Ｌｔｄ | Microbial fermentation of plant chemicals |
| CN106174221A (en) * | 2016-07-12 | 2016-12-07 | 江苏大学 | A kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food |
| CN106727730A (en) * | 2016-12-31 | 2017-05-31 | 健务（上海）生物科技有限公司 | Adjust biological bacterium solution of immunity of organisms anticancer and preparation method thereof |
| CN107699501A (en) * | 2017-11-24 | 2018-02-16 | 湖南新汇制药股份有限公司 | A kind of culture medium of Hericium erinaceus, bioconversion mycelium, the mycelial extract of bioconversion and application thereof |
| CN114891742A (en) * | 2022-06-23 | 2022-08-12 | 杭州中赢生物医疗科技有限公司 | Culture medium and in-vitro amplification method for NK (natural killer) cells with strong killing property |
| CN114891742B (en) * | 2022-06-23 | 2024-06-04 | 杭州中赢生物医疗科技有限公司 | Culture medium of NK cells with strong killing property and in-vitro amplification method |
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