CN108165421A - A kind of preparation method with the sealwort adlay health liquor for enhancing immune function - Google Patents

A kind of preparation method with the sealwort adlay health liquor for enhancing immune function Download PDF

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CN108165421A
CN108165421A CN201810229050.5A CN201810229050A CN108165421A CN 108165421 A CN108165421 A CN 108165421A CN 201810229050 A CN201810229050 A CN 201810229050A CN 108165421 A CN108165421 A CN 108165421A
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sealwort
adlay
fermentation
powder
product temperature
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马赞华
张伟国
钱和
马羚翔
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Jiangsu Fu Shou Bio Technology Co Ltd
Jiangnan University
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Jiangsu Fu Shou Bio Technology Co Ltd
Jiangnan University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/896Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8969Polygonatum (Solomon's seal)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/899Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A61K36/8994Coix (Job's tears)

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Abstract

The invention discloses a kind of preparation methods for the sealwort Yiren wine health liquor for having and enhancing immune function, belong to brewing technical field.The present invention handles sealwort, adlay both integration of drinking and medicinal herbs materials using the methods of crushings, boiling, extraction, enzymatic treatment, access strain ferments after control material-water ratio, is squeezed, filtered after fermentation, blending a kind of health liquor full of nutrition, having enhancing immune function of acquisition.The present invention proposes the preparation method of a kind of energy simplification of flowsheet, the sealwort adlay health liquor for improving raw material availability, and sealwort adlay health liquor obtained has invigorating the spleen to clear away damp pathogen, invigorating the lung and the kidney, the health-care efficacy for improving immunity etc..

Description

A kind of preparation method with the sealwort adlay health liquor for enhancing immune function
Technical field
The present invention relates to a kind of preparation methods for the sealwort adlay health liquor for having and enhancing immune function, belong to brewing technology Field.
Background technology
Contemporary people is higher and higher for the attention degree of health care, more than the elderly, and many young men open Begin to pursue health care, and be keen to buy health preserving health-care food, health liquor is exactly wherein more fast-selling one kind.
Sealwort is the Chinese medicine of the traditional integration of drinking and medicinal herbs in China, sweet in flavor, flat, has Buxue Yishen, reducing blood sugar and blood lipid, resists and swell The effects that knurl, immunological regulation.《Compendium of Materia Medica》Record " tonifying Qi kidney-nourishing, curing rheumatism, five viscera settling;Long term usage macrobiosis of making light of one's life by commiting suicide is not hungry;Mend five Labor seven kinds of impairments helps muscles and bones, resistance to cold and heat, embellish lung ", sealwort has very high medical value.The main component of sealwort has polysaccharide, soap Glycosides, anthraquinone, alkaloid etc. play the role of hypoglycemic, antifatigue, anti-oxidant and slow down aging, in fields such as medical health health cares All there is very high status.Adlay is also the medicinal and edible plant of national authentication, is known as " king of world grass ", in Doctor thinks that its is sweet in flavor, cool in nature, has effects that invigorating the spleen to clear away damp pathogen, tonifying lung heat-clearing, beauty treatment are stayed and holds.Very nutritious, the master of adlay The functional components wanted have coix seed oil, adlay ester, adlay polysaccharide etc., have antitumor, anticancer, adjust blood pressure and blood lipoid, improve and exempt from Epidemic disease power and other effects also has critically important status in fields such as medical health health cares.
Therefore, sealwort, adlay are prepared into sealwort adlay health liquor as raw material, good health-care effect can not only be reached, Also there is very big market.
At present, the number of being disclosed is the patent of invention of CN 103642648A:A kind of Yiren wine and preparation method thereof, it is open A kind of using adlay, fragrant standing grain glutinous rice, sorghum and rice husk is raw material, adds in what is obtained after liquor song diastatic fermentation is distilled again There is peppery mouth, top in 45 degree of wine, 45 degree of wine that the program obtains, be but not suitable for largely having as a kind of health liquor needing The crowd wanted drinks, if plus water blend and reduce its alcoholic strength, and very big destruction can be caused to its flavor, it is difficult to entrance;Also have The patent of invention of Publication No. CN 104130906A:A kind of sealwort glutinous rice wine formula and its brew method, use sealwort, jade Bamboo is main ingredient, repeatedly adds meal that liquid technology is added to brew the health liquor using glutinous rice, but the program need to get out polygonatum Solomon's seal in advance Extracting solution and liquid is decocted, multiple feeding during the fermentation, technique is relatively complicated, and wherein polygonatum Solomon's seal extracting solution is to pass through squeezing Juice obtains filtrate, can generate a large amount of residues, cause the waste of raw material.
It can be said that there are complex steps or beneficiating ingredient extraction effect are bad, raw material availability is not high for existing method The defects of waiting is protected therefore, it is necessary to develop the sealwort adlay that a kind of time-saving and efficiency, raw material availability is high, beneficiating ingredient content is high The preparation method of strong wine.
Invention content
To solve the above-mentioned problems, the present invention provides a kind of sealwort adlay health liquors with strengthen immunity Preparation method.This method extracts the active ingredient in sealwort first, carries out the processing of enzyme preparation together with adlay later, shortens work The utilization rate of raw material is improved while the skill time;In subsequent fermentations, addition liquid glucose enhances Huang to control alcoholic strength Essence, the utilization rate of adlay non-sugar ingredient, while make entire technological process easily controllable, improve the stability of product.
The object of the present invention is to provide a kind of preparation method of sealwort adlay health liquor with strengthen immunity, sheets The technical solution of invention is to be obtained sealwort using crushing, cellulase extraction processing sealwort and extracted liquid mixture, at crushing Reason adlay obtains lotus-seed-heart powder, and the sealwort handled well extraction liquid mixture is mixed to get sealwort adlay in proportion with lotus-seed-heart powder mixes Liquid obtains sealwort adlay saccharified liquid using boiling, α-amylase liquefaction, saccharification enzymatic conversion processing sealwort adlay mixed liquor, then will Obtain sealwort adlay saccharified liquid access strain fermentation, the mash through everfermentation through squeeze and filter, clarification or refined filtration, blend to obtain Sealwort adlay health liquor.
In one embodiment of the invention, what is used prepares raw material as sealwort and adlay;The sealwort rhizome requirement It rinses well;The adlay requires full grains, without moldy kernel;Sealwort elder generation stripping and slicing, then it is ground into 40-60 mesh with pulverizer Powder;The adlay is ground into the powder of 40-60 mesh with pulverizer.
In one embodiment of the invention, the cellulase extraction is to mix sealwort powder in proportion with water, so The cellulase of 50-100U/g is added in afterwards, 60-80min is handled under conditions of 45-55 DEG C, obtains sealwort extraction liquid mixture.
In one embodiment of the invention, the cellulase extraction is by sealwort powder and water in mass ratio 1:4-1: 6 ratio mixing.
In one embodiment of the invention, the cellulase extraction is by sealwort powder and water in mass ratio 1:5 Ratio mixes.
In one embodiment of the invention, it is mixed in proportion that sealwort, which extracts liquid mixture with lotus-seed-heart powder, in the method It closes;The time of the boiling is 15-20min;The α-amylase liquefaction is to add in the α-amylase of 80-120U/g at 80-95 DEG C Under the conditions of liquefy 60-80min;The saccharification enzymatic conversion is to add in 90-150U/g carbohydrase to be saccharified at a temperature of 58-65 DEG C 80-120min。
In one embodiment of the invention, sealwort extraction liquid mixture and lotus-seed-heart powder are in mass ratio in the method 1:1 ratio mixing.
In one embodiment of the invention, the strain includes Rhizopus oryzae and active dry yeast;The dry ferment of activity Main bag includes saccharomyces cerevisiae and aroma-producing yeasts.
In one embodiment of the invention, the Rhizopus oryzae uses mycelium inoculation;The saccharomyces cerevisiae inoculum concentration is 0.2-0.4%;The aroma-producing yeasts inoculum concentration is 0.3-0.6%.
In one embodiment of the invention, the fermentation is the fermented and cultured under conditions of 30 DEG C, by 12-14h It is harrowed into wardrobe, reduces product temperature, add in liquid glucose according to course of fermentation later and open rake once;After 7-9d, product temperature is close with room temperature, Fermentation stage terminates at this time.
In one embodiment of the invention, it the described method comprises the following steps:
Step 1 adds in water after sealwort is ground into sealwort powder, stirs evenly, and adds in the cellulase of 50-100U/g, 60-80min is extracted under conditions of 45-55 DEG C, obtains sealwort extraction liquid mixture;Adlay is ground into lotus-seed-heart powder;Sealwort is carried Liquid mixture is taken to carry out being mixed to get sealwort adlay mixed liquor in proportion with lotus-seed-heart powder;
Step 2, by sealwort adlay mixed liquor boiling 15-20min;Later adjust mixeding liquid temperature to 80-95 DEG C, with alkali/ It is 5.5-6.0 that acid solution, which adjusts pH, and the α-amylase for adding in 80-120U/g liquefies 60-80min under conditions of 80-95 DEG C;Again by liquid Mixeding liquid temperature after change is reduced to 58-65 DEG C, adds in 90-150U/g carbohydrase and is saccharified at 58-65 DEG C 80-120min, most Sealwort adlay saccharified liquid is obtained eventually;
Active dry yeast is activated 20-30min by step 3 under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
Step 3, inoculating active dry ferment and Rhizopus oryzae when sealwort adlay saccharified liquid product temperature is reduced to 26-30 DEG C, wine brewing Yeast-inoculated amount is 0.2-0.4%, and aroma-producing yeasts inoculum concentration is 0.3-0.6%, and Rhizopus oryzae uses mycelium inoculation, in 30 DEG C of item Primary fermentation culture under part is harrowed by 12-14h into wardrobe, is reduced product temperature, is added in fermentation process later according to course of fermentation sugared Liquid simultaneously opens rake once, it is therefore an objective to reduce product temperature and fermentation is made uniformly to carry out;After 7-9d, product temperature is close with room temperature, ferments at this time Stage terminates;
Step 4 by the mash squeeze and filter through everfermentation, obtains filtrate, stands clarification under cryogenic or uses diatom Native refined filtration is blent after 90-95 DEG C of sterilizing as finished wine.
In one embodiment of the invention, the mass ratio of sealwort powder and water is 1 in the step 1:4-1:6.
In one embodiment of the invention, the mass ratio of sealwort powder and water is 1 in the step 1:5.
In one embodiment of the invention, sealwort extraction liquid mixture and lotus-seed-heart powder are in mass ratio in the method 1:1 ratio mixing.
The present invention provides a kind of sealwort adlay health liquors being prepared according to the above method.
Advantageous effect:
(1) this health liquor that the present invention obtains has the function of there is hypoglycemic, antifatigue, anti-oxidant and anti-aging etc. The effect of, adlay has effects that antitumor, anticancer, adjusts blood pressure and blood lipoid, improves immunity etc., is prepared using the two as raw material Health liquor have very strong invigorating the spleen to clear away damp pathogen, invigorating the lung and the kidney, strengthen immunity healthcare function.
(2) present invention improves raw material availability, after optimization using cellulase extraction pretreatment sealwort raw material Cellulase treatment conditions can improve the recovery rate of saponin(e and polysaccharide in sealwort, and up to 34.22%, polysaccharide carries saponin extraction rate Rate is taken up to 10.24%, higher than water-bath extraction method;The present invention mixes after extraction with adlay carries out enzymatic treatment, further improves Raw material availability, the active principle saponin(e in sealwort have been respectively increased 54.3%, 68.6% with polysaccharide, and pass through optimize it is yellow Essence extraction and enzyme treatment process parameter shorten the process time of 20-30%.
(3) saccharification liquefaction process of the invention is mainly the grape that the starch in raw material is decomposed into yeast and can be utilized Sugar can obtain preferable saccharification liquefaction effect using the saccharification liquefaction process after optimization, and DE values reach 78.57%.
(4) present invention controls fermentation time and alcoholic strength by adding liquid glucose mode during the fermentation, and the method can be with Alcoholic strength is controlled to improve the stability of quality between product batches in optimum range.
(5) present invention can enhance ferment effect, while increase to health liquor using yeast and Rhizopus oryzae mixed fungus fermentation Unique flavor.
(6) sealwort of the invention used, adlay have characteristic sweet in flavor in itself, can largely alleviate the peppery mouth of alcohol, The problem of being difficult into larynx, the health liquor moderate taste prepared using sealwort, adlay are suitble to majority of populations reference.
Description of the drawings
Fig. 1 is the preparation process flow diagram of the sealwort adlay health liquor of the present invention.
Specific embodiment
The present invention is specifically described with comparative example with reference to embodiment.
Embodiment 1
The preparation method that embodiment 1 uses is as follows:
(1) 5 times of water is added in after sealwort is crushed into powder, stirs evenly, adds in the cellulase of 100U/g, at 52 DEG C Under the conditions of extract 80min, obtain sealwort extraction liquid mixture;In mass ratio 1:1 adds in lotus-seed-heart powder, and boiling 20min is adjusted later Mixed material temperature is to 85 DEG C, and it is 5.5 to adjust pH with alkali/acid solution, and the α-amylase for adding in 120U/g liquefies under conditions of 95 DEG C 80min;Temperature is reduced to 58 DEG C in next step, adds in carbohydrase, dosage 120U/g, processing time 100min are finally obtained Obtain sealwort adlay saccharified liquid;
(2) active dry yeast activates 30min under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
(3) inoculation yeast and Rhizopus oryzae when material product temperature is reduced to 30 DEG C, saccharomyces cerevisiae inoculum concentration are 0.4%, raw perfume ferment Female inoculum concentration is 0.6%, and Rhizopus oryzae uses mycelium inoculation, and the primary fermentation culture under conditions of 30 DEG C is harrowed by 14h into wardrobe, Product temperature is reduced, liquid glucose is added according to course of fermentation in fermentation process later and opens rake once, it is therefore an objective to reduce product temperature and make hair Ferment uniformly carries out;After 9d, product temperature is close with room temperature, and fermentation stage terminates at this time;
(4) by the mash squeeze and filter through everfermentation, filtrate is obtained, stands clarification or smart with diatomite under cryogenic Filter is blent after 90 DEG C of sterilizings as finished wine;
(5) finished wine alcoholic strength reaches 13.47 degree, acidity 3.5g/L, polysaccharide 15.86g/L, saponin(e 145mg/100mL, wine Body is in golden yellow, the mellow harmony of flavor.
Embodiment 2
The preparation method that embodiment 2 uses is as follows:
(1) 4 times of water is added in after sealwort is crushed into powder, stirs evenly, adds in the cellulase of 80U/g, at 55 DEG C Under the conditions of extract 60min, obtain sealwort extraction liquid mixture;In mass ratio 1:1 adds in lotus-seed-heart powder, and boiling 20min is adjusted later Mixed material temperature is to 90 DEG C, and it is 5.5 to adjust pH with alkali/acid solution, and the α-amylase for adding in 100U/g liquefies under conditions of 90 DEG C 60min;Temperature is reduced to 60 DEG C in next step, adds in carbohydrase, dosage 120U/g, processing time 90min are final to obtain Sealwort adlay saccharified liquid;
(2) active dry yeast activates 20min under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
(3) inoculation yeast and Rhizopus oryzae when material product temperature is reduced to 30 DEG C, saccharomyces cerevisiae inoculum concentration are 0.3%, raw perfume ferment Female inoculum concentration is 0.4%, and Rhizopus oryzae uses mycelium inoculation, and the primary fermentation culture under conditions of 30 DEG C is harrowed by 12h into wardrobe, Product temperature is reduced, liquid glucose is added according to course of fermentation in fermentation process later and opens rake once, it is therefore an objective to reduce product temperature and make hair Ferment uniformly carries out;After 8d, product temperature is close with room temperature, and fermentation stage terminates at this time;
(4) by the mash squeeze and filter through everfermentation, filtrate is obtained, stands clarification or smart with diatomite under cryogenic Filter is blent after 95 DEG C of sterilizings as finished wine;
(5) finished wine alcoholic strength reaches 12.97 degree, acidity 3.9g/L, polysaccharide 16.26g/L, saponin(e 136mg/100mL, wine Body is in golden yellow, the mellow harmony of flavor.
Embodiment 3
The preparation method that embodiment 3 uses is as follows:
(1) 5 times of water is added in after sealwort is crushed into powder, stirs evenly, adds in the cellulase of 50U/g, at 45 DEG C Under the conditions of extract 75min, obtain sealwort extraction liquid mixture;In mass ratio 1:1 adds in lotus-seed-heart powder, and boiling 15min is adjusted later Mixed material temperature is to 95 DEG C, and it is 6.0 to adjust pH with alkali/acid solution, and the α-amylase for adding in 80U/g liquefies under conditions of 95 DEG C 70min;Temperature is reduced to 65 DEG C in next step, adds in carbohydrase, dosage 150U/g, processing time 80min are final to obtain Sealwort adlay saccharified liquid;
(2) active dry yeast activates 30min under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
(3) inoculation yeast and Rhizopus oryzae when material product temperature is reduced to 26 DEG C, saccharomyces cerevisiae inoculum concentration are 0.2%, raw perfume ferment Female inoculum concentration is 0.3%, and Rhizopus oryzae uses mycelium inoculation, and the primary fermentation culture under conditions of 30 DEG C is harrowed by 13h into wardrobe, Product temperature is reduced, liquid glucose is added according to course of fermentation in fermentation process later and opens rake once, it is therefore an objective to reduce product temperature and make hair Ferment uniformly carries out;After 9d, product temperature is close with room temperature, and fermentation stage terminates at this time;
(4) by the mash squeeze and filter through everfermentation, filtrate is obtained, stands clarification or smart with diatomite under cryogenic Filter is blent after 90 DEG C of sterilizings as finished wine;
(5) finished wine alcoholic strength reaches 13.41 degree, acidity 3.7g/L, polysaccharide 13.76g/L, saponin(e 152mg/100mL, wine Body is in golden yellow, the mellow harmony of flavor.
Comparative example 1
Sealwort is anticipated without using cellulase, directly using boiling extraction fermentation raw material.
(1) 4 times of water is added in after sealwort is crushed into powder, stirs evenly, 60min is extracted under conditions of 60 DEG C, is obtained Sealwort extracts liquid mixture;In mass ratio 1:1 adds in lotus-seed-heart powder, and boiling 20min adjusts mixed material temperature to 90 DEG C later, It is 5.5 to adjust pH with alkali/acid solution, and the α-amylase for adding in 100U/g liquefies 60min under conditions of 90 DEG C;Temperature is dropped in next step Low is 60 DEG C, adds in carbohydrase, dosage 120U/g, processing time 90min are final to obtain sealwort adlay saccharified liquid.
(2) active dry yeast activates 20min under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
(3) inoculation yeast and Rhizopus oryzae when material product temperature is reduced to 30 DEG C, saccharomyces cerevisiae inoculum concentration are 0.3%, raw perfume ferment Female inoculum concentration is 0.4%, and Rhizopus oryzae uses mycelium inoculation, and the primary fermentation culture under conditions of 30 DEG C is harrowed by 12h into wardrobe, Product temperature is reduced, liquid glucose is added according to course of fermentation in fermentation process later and opens rake once, it is therefore an objective to reduce product temperature and make hair Ferment uniformly carries out;After 8d, product temperature is close with room temperature, and fermentation stage terminates at this time;
(4) by the mash squeeze and filter through everfermentation, filtrate is obtained, stands clarification or smart with diatomite under cryogenic Filter is blent after 95 DEG C of sterilizings as finished wine;
(5) finished wine alcoholic strength made from reaches 12.54%, acidity 3.1g/L, polysaccharide 10.35g/L, saponin(e 85mg/ 100mL, sealwort nutritive pan content of material significantly reduce.
Comparative example 2
The preparation method that comparative example 2 uses is as follows:Change sealwort Extraction technique
(1) 4 times of water is added in after sealwort is crushed into powder, stirs evenly, adds in the cellulase of 180U/g, at 65 DEG C Under the conditions of extract 90min, obtain sealwort extraction liquid mixture;In mass ratio 1:1 adds in lotus-seed-heart powder, and boiling 15min is adjusted later Mixed material temperature is to 95 DEG C, and it is 6.0 to adjust pH with alkali/acid solution, and the α-amylase for adding in 80U/g liquefies under conditions of 95 DEG C 70min;Temperature is reduced to 65 DEG C in next step, adds in carbohydrase, dosage 150U/g, processing time 80min are final to obtain Sealwort adlay saccharified liquid;
(2) active dry yeast activates 30min under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
(3) inoculation yeast and Rhizopus oryzae when material product temperature is reduced to 30 DEG C, saccharomyces cerevisiae inoculum concentration are 0.2%, raw perfume ferment Female inoculum concentration is 0.4%, and Rhizopus oryzae uses mycelium inoculation, and the primary fermentation culture under conditions of 30 DEG C is harrowed by 12h into wardrobe, Product temperature is reduced, liquid glucose is added according to course of fermentation in fermentation process later and opens rake once, it is therefore an objective to reduce product temperature and make hair Ferment uniformly carries out;After 9d, product temperature is close with room temperature, and fermentation stage terminates at this time;
(4) by the mash squeeze and filter through everfermentation, filtrate is obtained, stands clarification or smart with diatomite under cryogenic Filter is blent after 90 DEG C of sterilizings as finished wine;
(5) finished wine alcoholic strength reaches 13.24 degree, acidity 4.1g/L, polysaccharide 7.39g/L, saponin(e 93mg/100mL, saponin(e Content is relatively low, reduces health value.
Comparative example 3
The preparation method that comparative example 3 uses is as follows:Change fermentation condition
(1) 5 times of water is added in after sealwort is crushed into powder, stirs evenly, adds in the cellulase of 80U/g, at 52 DEG C Under the conditions of extract 80min, obtain sealwort extraction liquid mixture;In mass ratio 1:1 adds in lotus-seed-heart powder, and boiling 18min is adjusted later Mixed material temperature is to 92 DEG C, and it is 5.8 to adjust pH with alkali/acid solution, and the α-amylase for adding in 120U/g liquefies under conditions of 95 DEG C 80min;Temperature is reduced to 60 DEG C in next step, adds in carbohydrase, dosage 120U/g, processing time 90min are final to obtain Sealwort adlay saccharified liquid;
(2) active dry yeast activates 30min under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
(3) inoculation yeast and Rhizopus oryzae when material product temperature is reduced to 35 DEG C, saccharomyces cerevisiae inoculum concentration are 0.5%, raw perfume ferment Female inoculum concentration is 1.0%, and Rhizopus oryzae uses mycelium inoculation, and the primary fermentation culture under conditions of 35 DEG C is harrowed by 12h into wardrobe, Product temperature is reduced, liquid glucose is added according to course of fermentation in fermentation process later and opens rake once, it is therefore an objective to reduce product temperature and make hair Ferment uniformly carries out;After 8d, product temperature is close with room temperature, and fermentation stage terminates at this time;
(4) by the mash squeeze and filter through everfermentation, filtrate is obtained, stands clarification or smart with diatomite under cryogenic Filter is blent after 95 DEG C of sterilizings as finished wine;
(5) finished wine alcoholic strength reaches 9.43 degree, acidity 16.8g/L, polysaccharide 6.63g/L, saponin(e 141mg/100mL, wine Body is in golden yellow, and wine degree is relatively low, and acidity is higher, and wine taste is discord.
Comparative example 4
The preparation method that comparative example 4 uses is as follows:Without supplement liquid glucose in course of fermentation
(1) 5 times of water is added in after sealwort is crushed into powder, stirs evenly, adds in the cellulase of 50U/g, at 45 DEG C Under the conditions of extract 75min, obtain sealwort extraction liquid mixture;In mass ratio 1:1 adds in lotus-seed-heart powder, and boiling 15min is adjusted later Mixed material temperature is to 95 DEG C, and it is 5.8 to adjust pH with alkali/acid solution, and the α-amylase for adding in 80U/g liquefies under conditions of 95 DEG C 70min;Temperature is reduced to 65 DEG C in next step, adds in carbohydrase, dosage 150U/g, processing time 80min are final to obtain Sealwort adlay saccharified liquid;
(2) active dry yeast activates 30min under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
(3) inoculation yeast and Rhizopus oryzae when material product temperature is reduced to 26 DEG C, saccharomyces cerevisiae inoculum concentration are 0.2%, raw perfume ferment Female inoculum concentration is 0.3%, and Rhizopus oryzae uses mycelium inoculation, and the primary fermentation culture under conditions of 30 DEG C is harrowed by 12h into wardrobe, Reduce product temperature, it is therefore an objective to reduce product temperature and fermentation is made uniformly to carry out;After 8d, product temperature is close with room temperature, at this time fermentation stage knot Beam;
(4) by the mash squeeze and filter through everfermentation, filtrate is obtained, stands clarification or smart with diatomite under cryogenic Filter is blent after 90 DEG C of sterilizings as finished wine;
(5) finished wine alcoholic strength reaches 10.41 degree, acidity 6.2g/L, polysaccharide 8.58g/L, saponin(e 149mg/100mL, wine Precision and polyoses content are relatively low, and flavor is discord.
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not limited to the present invention, any to be familiar with this skill The people of art without departing from the spirit and scope of the present invention, can do various change and modification, therefore the protection model of the present invention Enclosing be subject to what claims were defined.

Claims (10)

1. a kind of preparation method with the sealwort adlay health liquor for enhancing immune function, which is characterized in that the method is to adopt Sealwort extraction liquid mixture is obtained with crushing, cellulase extraction processing sealwort, lotus-seed-heart powder is obtained using pulverization process adlay, it will The sealwort extraction liquid mixture handled well is mixed to get sealwort adlay mixed liquor in proportion with lotus-seed-heart powder, using boiling, α-amylase Liquefaction, saccharification enzymatic conversion processing sealwort adlay mixed liquor obtain sealwort adlay saccharified liquid, and then obtained sealwort adlay is saccharified Liquid access strain fermentation, the mash through everfermentation through squeeze and filter, clarification or refined filtration, blend to obtain sealwort adlay health liquor.
2. according to the method described in claim 1, it is characterized in that, what is used prepares raw material as sealwort and adlay;The sealwort Rhizome requires to rinse well;The adlay requires full grains, without moldy kernel;Sealwort elder generation stripping and slicing, then crushed with pulverizer Into the powder of 40-60 mesh;The adlay is ground into the powder of 40-60 mesh with pulverizer.
3. method according to claim 1 or 2, which is characterized in that cellulase extraction be by sealwort powder and water by Ratio mixes, and then adds in the cellulase of 50-100U/g, and 60-80min is handled under conditions of 45-55 DEG C, obtains sealwort and carries Take liquid mixture.
4. according to any methods of claim 1-3, which is characterized in that the cellulase extraction is by sealwort powder and water In mass ratio 1:4-1:6 ratio mixing.
5. according to any methods of claim 1-4, which is characterized in that sealwort extraction liquid mixture and the heart of a lotus seed in the method Benevolence powder is to mix in proportion;The time of the boiling is 15-20min;The α-amylase liquefaction is to add in the liquefaction of 80-120U/g Enzyme liquefies 60-80min under conditions of 80-95 DEG C;The saccharification enzymatic conversion is to add in 90-150U/g carbohydrase at 58-65 DEG C At a temperature of be saccharified 80-120min.
6. according to any methods of claim 1-5, which is characterized in that the strain includes Rhizopus oryzae and the dry ferment of activity It is female;The active dry yeast includes saccharomyces cerevisiae and aroma-producing yeasts.
7. according to the method described in claim 6, it is characterized in that, the Rhizopus oryzae uses mycelium inoculation;The saccharomyces cerevisiae Inoculum concentration is 0.2-0.4%;The aroma-producing yeasts inoculum concentration is 0.3-0.6%.
8. according to any methods of claim 1-7, which is characterized in that the fermentation is training of fermenting under conditions of 30 DEG C It is foster, it is harrowed by 12-14h into wardrobe, reduces product temperature, added in liquid glucose according to course of fermentation later and open rake once;After 7-9d, product temperature It is close with room temperature, fermentation stage terminates at this time.
9. according to any methods of claim 1-8, which is characterized in that include the following steps:
Step 1 adds in water after sealwort is ground into sealwort powder, stirs evenly, and the cellulase of 50-100U/g is added in, in 45- 60-80min is extracted under conditions of 55 DEG C, obtains sealwort extraction liquid mixture;Adlay is ground into lotus-seed-heart powder;By sealwort extracting solution Mixture carries out being mixed to get sealwort adlay mixed liquor in proportion with lotus-seed-heart powder;
Step 2, by sealwort adlay mixed liquor boiling 15-20min;Mixeding liquid temperature is adjusted later to 80-95 DEG C, with alkali/acid solution Adjusting pH is 5.5-6.0, and the α-amylase for adding in 80-120U/g liquefies 60-80min under conditions of 80-95 DEG C;After liquefying again Mixeding liquid temperature be reduced to 58-65 DEG C, add in 90-150U/g carbohydrase and be saccharified at 58-65 DEG C 80-120min, finally obtain Obtain sealwort adlay saccharified liquid;
Active dry yeast is activated 20-30min by step 3 under the conditions of 37 DEG C of the aqueous solution containing 2% sugar;
Step 3, inoculating active dry ferment and Rhizopus oryzae, saccharomyces cerevisiae when sealwort adlay saccharified liquid product temperature is reduced to 26-30 DEG C Inoculum concentration is 0.2-0.4%, and aroma-producing yeasts inoculum concentration is 0.3-0.6%, and Rhizopus oryzae uses mycelium inoculation, under conditions of 30 DEG C Primary fermentation culture is harrowed, reduction product temperature by 12-14h into wardrobe, and liquid glucose is added in simultaneously according to course of fermentation in fermentation process later It is primary to open rake, it is therefore an objective to reduce product temperature and fermentation is made uniformly to carry out;After 7-9d, product temperature is close with room temperature, at this time fermentation stage Terminate;
Step 4 by the mash squeeze and filter through everfermentation, obtains filtrate, stands clarification or smart with diatomite under cryogenic Filter is blent after 90-95 DEG C of sterilizing as finished wine.
10. the sealwort adlay health liquor being prepared according to any methods of claim 1-9.
CN201810229050.5A 2018-03-20 2018-03-20 A kind of preparation method with the sealwort adlay health liquor for enhancing immune function Pending CN108165421A (en)

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