CN1142283C - Process for preparing activated polyose by biologic technique and its product - Google Patents
Process for preparing activated polyose by biologic technique and its product Download PDFInfo
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- CN1142283C CN1142283C CNB011068159A CN01106815A CN1142283C CN 1142283 C CN1142283 C CN 1142283C CN B011068159 A CNB011068159 A CN B011068159A CN 01106815 A CN01106815 A CN 01106815A CN 1142283 C CN1142283 C CN 1142283C
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Abstract
The present invention relates to a method for preparing active polysaccharide by biotechnology and products of the present invention. The method comprises the following steps: firstly, traditional Chinese medicine which is the source of both food and medicine is adopted as a culture medium for inoculating a composite fungus strain of lucid ganoderma, saccharomycete, cordyceps, etc. so as to carry out biologic fermentation, and cultivation is carried out at the temperature of 15 to 28 DEG C for 17 to 25 days after inoculation; secondly, the fermentation is terminated when the amount of fungus polysaccharide reaches a first peak value, and dehydration is then carried out so as to biochemically extract the active polysaccharide or to carry out drying and crushing; finally, finished products are obtained by sterilization, quality inspection and packaging. The technology of the present invention makes the fungus polysaccharide of the cordyceps, the lucid ganoderma, etc. suitable for industrialized production as well as makes macromolecular compounds of the traditional Chinese medicine biodegradable, and improves the medicine effects of the traditional Chinese medicine. In addition, the products of the present invention also has the advantages of improved immunizing power of human bodies, cancer preventing and resisting effects and no toxicity or side effects to the human bodies.
Description
The present invention relates to a kind of biotechnology, refer to especially carry out biological fermentation, produce the method for fungus polysaccharide with herbal medicine and fungi.
Studies have shown that in a large number that both at home and abroad medicinal fungus such as Chinese caterpillar fungus, glossy ganoderma have significant inhibition lethal effect to cancer cells.Recent studies proves that Cordyceps polysaccharide, cordycepin are good biological regulator, antimutagens, can strengthen immune function of human body, improves immunocompetence.Its antitumour activity is the combination rate that improves normal people and leukemia NK cells of human beings activity and K562 cell, and the direct killing tumour cell stops cancer cells to increase.In addition, also have reducing blood lipid, hemopoietic system green blood improves oxyphorase and hemochrome; Also can correct myocardial ischemia, improve effects such as coronary artery circulation, treatment arrhythmia, anticoagulant, antithrombotic formation.Ganoderma lucidum seu Japonicum, the effect of liver-kidney tonifying is arranged, promoting longevity.Ganoderan can suppress tumour, mediator's body immunity function, two-ways regulation blood pressure; promote the human endocrine balance, the hemopoietic system nourishes blood, and the protection liver cell avoids poisoning and damages; suppress thrombosis, disintegrate effects such as on every side thrombus of tissue, blood fat reducing, blood viscosity lowering.
Medicinal fungus such as Chinese caterpillar fungus, glossy ganoderma resource is few, adds that people are unreasonable to excavate medicine source deficiency.Therefore cost an arm and a leg, be difficult to the people of wage-earners and utilize.People when illnesss such as treatment diabetes, cardiovascular diseases, conventional Chinese medicine.Though some curative effects are arranged, because some macromolecular substance of Chinese medicine fail to degrade, people absorb few, and drug effect fails to give full play to, so curative effect is limited.Therefore, in bio-science growing today, people have carried out a large amount of research to artificial propagation and the processing and utilization of Chinese caterpillar fungus, glossy ganoderma.The patent application of relevant this respect reaches tens of.For example application number is 93110814 " beverage made of natural edible and medicinal mushroom ", utilizes edible medicinal fungus do fermentation body spontaneous fermentation breedings in dark basic culture medium solution such as glossy ganoderma.Application number is 89104005 " continuous fermentation of Chinese caterpillar fungus (cephalo-mycelium) with sporothrix ".Application number is 96103521 " Chinese medicine nutrition oral administration ", is prepared from addition of Chinese yam, Chinese holly reading notes, Spina Date Seed and Radix Glycyrrhizae with glossy ganoderma, Hericium erinaceus (Bull. Ex Fr.) Pers. etc." product of edible mushrooms and herbal medicine mixed fermentation brewing and the method " of application number 93104737, adopting edible mushrooms and the effective prescription of a certain traditional Chinese medicine for oral administration is raw material, makes bacterial classification with the edible root certain kind of berries, through the multiple operation fermented soy, produce the ethanol of 3-5%, get mash and make commodity.
The objective of the invention is: solve the technical barrier of Chinese caterpillar fungus, glossy ganoderma artificial propagation, provide batch production to produce the technology of fungus polysaccharide and the Chinese medicinal materials macromolecular cpd is degraded and improve the technology of drug effect.
Another object of the present invention is: fungus polysaccharide goods and fungus polysaccharide and herbal medicine composite product are provided, produce the health care pharmaceuticals that existing high-grade nutrient health care effect has certain targeted therapy effect again.
The object of the present invention is achieved like this, and production technology is to be raw material with the effective prescription of herbal medicine for oral administration, and raw material is behind pre-treatment, sterilization, make substratum, fungi strains such as Chinese caterpillar fungus, glossy ganoderma are mixed the back inoculation, the solid biologic fermentation, culture temperature was cultivated 17-25 days down for 15～28 ℃.When reaching first peak value, fungus polysaccharide content stops cultivating, dehydration then, and biochemical extraction active polysaccharide, or dehydrate, pulverize, quality inspection again, sterilization, packing.
Its product is according to different the having any different of composite bacteria of composition of raw materials and inoculation.
The present invention one has solved Chinese caterpillar fungus, the technology of glossy ganoderma artificial propagation and the technology that fungus polysaccharide is produced in batch production; The 2nd, adopt solid biologic fermentation and composite bacteria inoculation, make the macromolecular cpd degraded of herbal medicine, be beneficial to absorption, improve drug effect.For entering the ordinary people man, precious medicines such as Chinese caterpillar fungus opened up road, for people provide the high-class healthy product.
Concrete grammar of the present invention is as follows: accompanying drawing is seen in technical process, and is as follows below in conjunction with description of drawings.
First operation: with herbal prescription 1,, promptly rogue, be ground into 30～50 orders after the impurity elimination, dehydration, add water 50～150% by the raw material gross weight again Chinese medicine material pre-treatment 2, pH value 5.5～8, bottling or pack are at 0.8～1.5kg/cm
2Following sterilization 1-4 hour (in the accompanying drawing 3), cooling promptly gets substratum.
Second operation: preparation composite bacteria 4, composite bacteria is mixed and is formed by yeast, glossy ganoderma, Chinese caterpillar fungus, mushroom fungi, and the consumption of each bacterial classification can be similar and different.Inoculate composite bacteria with the substratum that first operation makes under aseptic condition, inoculum size is the 1-5% of substratum.Inoculation back solid biologic fermentation 5, promptly controlled temperature was cultivated 17～25 days down at 15～28 ℃, stopped cultivating when fungus polysaccharide content reaches first peak value.
The 3rd operation: dehydration, biochemical extraction fungus polysaccharide or grinding and processing become product.Raw material behind the biological fermentation is being reached dehydration below 70 ℃, and water content is controlled at below 15%, then according to the difference of raw material, can take way, quality inspection sterilization 10, the packing 11 of biochemical extraction 8 or dry (expanded) pulverizing 9 promptly to get this finished product 12 respectively.
With following 11 kinds of Chinese prescriptions, raw material weight ratio be:
Chinese caterpillar fungus, sealwort, Chinese yam, the bighead atractylodes rhizome, Glossy Privet Fruit, each 2-5 part of the red sage root, wolfberry fruit, shizandra berry, Herba Epimedii, each 1-4 part of Herba Epimedii, coptis 1-3 part.Rogue, impurity elimination, dehydration pulverize, and adds water 100% by the raw material gross weight again, pH value 5.5～6.5, bottling or pack, sterilization is at 1.0～1.5kg/cm
23 hours, the cooling back was at aseptic indoor inoculation composite bacteria.Composite bacteria is made up of yeast, glossy ganoderma, three kinds of mixing of mushroom, and the consumption of each bacterial classification equates.Cultivated 20～25 days at 20～28 ℃ of following alternating temperatures the inoculation back, stops cultivating the biochemical then active polysaccharide that extracts of dehydration (water content≤10%) when fungus polysaccharide content reaches first peak value.Last quality inspection, ultraviolet disinfection pack or go into capsule.The preferable weight ratio of each Chinese medicine material of this product is: respectively 4 parts of Chinese caterpillar fungus, sealwort, Chinese yam, the bighead atractylodes rhizome, Glossy Privet Fruit, the reds sage root, wolfberry fruit, shizandra berry, Herba Epimedii, each 2.5 parts of Herba Epimediis, 1.5 parts of the coptiss.It is respond well that this product is used for diabetes, is again first-class healthcare products.Share with other products, of many uses.
Implementation column 2 Chinese caterpillar funguses, glossy ganoderma body-building electuary
With following several Chinese prescriptions make substratum, each raw material weight ratio is:
Mushroom, black Semen Maydis, each 3-10 part of bitter buckwheat, Poria cocos, matrimony vine, each 0.5-3 part of rheum officinale, red sage root 0.4-8 part, root of kudzu vine 0.3-3 part, tuber of multiflower knotweed 0.3-3 part, pearl 0.3-2 part, black soya bean 2-8 part, gynostemma pentaphylla 0.8-8 part.
Raw material is rogued, impurity elimination, dehydration are pulverized, and adds water 120% by the raw material gross weight then, and pH value 5～7 is at 1.2kg/cm
2Under sterilized naturally cooling 3.5 hours; Yeast, glossy ganoderma, Cordyceps are mixed the back inoculation.15～28 ℃ of culture temperature were cultivated 17～25 days, stop to cultivate when fungus polysaccharide content reaches first peak value, dehydrated in 70 ℃, and water content reduces to 12% when following, extruding puffing, pulverizing, ultraviolet disinfection, quality inspection again, the packing of weighing.This product Chinese medicine material weight preferred proportion is: respectively 7 parts of mushroom, black Semen Maydis, bitter buckwheats, respectively 2 parts of Poria cocos, matrimony vine, rheum officinales, 6 parts of the reds sage root, 2 parts of the roots of kudzu vine, 2.5 parts of the tubers of multiflower knotweed, 1.5 parts of pearls, 6 parts in black soya bean, 7.5 parts of gynostemma pentaphylla.This product mixes with Chinese caterpillar fungus, ganoderan to be taken, good to the result of treatment of cardiovascular disorder.
The preparation of embodiment 3 Ganoderma teas
With the aforesaid method difference.The one, the culture medium raw material weight ratio is: Ginkgo Leaf, gynostemma pentaphylla, each 4-8 part of tealeaves, glossy ganoderma, pearl, mushroom, each 1-5 part of the red sage root.The 2nd, with yeast, Cordyceps combined inoculation.The 3rd, meal or fine powder granulation when pulverizing.This product mixes with Cordyceps polysaccharide to be taken, and treatment hepatitis B curative effect is obvious.Each Chinese medicine weight preferred proportion of this product is: respectively 5 parts of Ginkgo Leaf, gynostemma pentaphylla, tealeaves, respectively 2 parts of glossy ganoderma, pearl, mushroom, the reds sage root.
Embodiment 4 glossy ganoderma peptic or capsules, its culture medium raw material weight ratio is: glutinous rice 1-3 part, dried orange peel 1-3 part, pearl 1-2 part, method summer 1-2 part, glossy ganoderma 2-4 part, coptis 0.5-1 part.Composite bacteria is made up of yeast, hedgehog hydnum bacterial classification.
The present invention with medication, dietotherapy is molten is one.Its product is a natural product.Its feature is: 1. have no side effect; 2. improve the immunizing power of human body, its antibody cell count in serum hemolysin and the spleen that can raise improves serum immunoglobulin level; can activate the phagocytic function of reticuloendothelial system; activating macrophage promotes the generation of TNK and IL-2, promotes human organ function normalizing.3. Worm grass and glossy ganoderma polysaccharides is used for glycosuria sugar, and the Chinese caterpillar fungus Ganoderma granule is used for the treatment of " hypertension " " coronary heart disease " etc., and efficient up to more than 95%, clinical cure rate is greater than 60%, and is evident in efficacy.
Claims (1)
1. with the method for preparing activated polyose by biologic technique, it is characterized in that:
A. prepare substratum, the raw material weight ratio of substratum is: Chinese caterpillar fungus, sealwort, Chinese yam, the bighead atractylodes rhizome, Glossy Privet Fruit, each 2-5 part of the red sage root, wolfberry fruit, shizandra berry, Herba Epimedii, each 1-4 part of Herba Epimedii, coptis 1-3 part, with being ground into the 30-50 order after the above-mentioned raw materials dehydration, add water 50～150% by the raw material gross weight again, pH value 5.5-6.5, bottling or pack are at 1.0-1.5kg/cm
2Pressure was sterilized 1-4 hour down, then naturally cooling;
B. yeast, Ganderma lucidum, Lentinus Edodes fungus are mixed, be inoculated under aseptic condition on the above-mentioned substratum, inoculum size is the 1-5% of substratum, 20～28 ℃ of temperature bottom fermentations 20-25 days, stops cultivation and fermentation when fungus polysaccharide reaches first peak value;
C. above-mentioned cultivation and fermentation thing is being dewatered below 70 ℃, water content is controlled at below 15%, extracts its granulose with prior art, sterilization, packing.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB011068159A CN1142283C (en) | 2001-01-03 | 2001-01-03 | Process for preparing activated polyose by biologic technique and its product |
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| CNB011068159A CN1142283C (en) | 2001-01-03 | 2001-01-03 | Process for preparing activated polyose by biologic technique and its product |
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| CN1302902A CN1302902A (en) | 2001-07-11 |
| CN1142283C true CN1142283C (en) | 2004-03-17 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100390295C (en) * | 2005-11-26 | 2008-05-28 | 山西大学 | Microorganism polysaccharide and its preparation method and application |
| CN101524374B (en) * | 2008-03-04 | 2012-01-11 | 德阳市食用菌专家大院 | Auricularia Polytricha(Mont)Sacl fermentation product, oral preparation containing same, preparation method and application |
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| CN1307314C (en) * | 2004-04-28 | 2007-03-28 | 中国食品发酵工业研究院 | Enzymolytic preparation method for glossy ganoderma amylose |
| CN1317391C (en) * | 2004-07-01 | 2007-05-23 | 阎波 | Method for extracting ganoderan by solid fermentation |
| CN1308350C (en) * | 2004-07-28 | 2007-04-04 | 武汉大学 | Process for preparing tuckahoe mycelium extracellular polysacchride with anti-tumor activity |
| CN102078016A (en) * | 2010-11-25 | 2011-06-01 | 怀宝城 | Glossy ganoderma and Chinese yam beverage and preparation method thereof |
| CN102599481A (en) * | 2012-03-12 | 2012-07-25 | 杨毅 | Method for preparing solid fermentation mixed edible medicinal fungus sporocarp |
| CN103183740B (en) * | 2013-03-22 | 2015-06-10 | 李士刚 | Production method of fructus schisandrae polysaccharide |
| CN104055184B (en) * | 2014-06-27 | 2015-07-08 | 杭州雪域生物技术有限公司 | Hirsutella sinensis hypolipidemic healthcare beverage and preparation method thereof |
| CN105943577B (en) * | 2016-04-29 | 2018-02-13 | 刘东波 | The microbial fermentation of autonomic drug |
| CN106174221A (en) * | 2016-07-12 | 2016-12-07 | 江苏大学 | A kind of method utilizing Ganoderma to convert Rhizoma Dioscoreae production functional food |
| CN106727730A (en) * | 2016-12-31 | 2017-05-31 | 健务（上海）生物科技有限公司 | Adjust biological bacterium solution of immunity of organisms anticancer and preparation method thereof |
| CN107699501A (en) * | 2017-11-24 | 2018-02-16 | 湖南新汇制药股份有限公司 | A kind of culture medium of Hericium erinaceus, bioconversion mycelium, the mycelial extract of bioconversion and application thereof |
| CN114891742B (en) * | 2022-06-23 | 2024-06-04 | 杭州中赢生物医疗科技有限公司 | Culture medium of NK cells with strong killing property and in-vitro amplification method |
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2001
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100390295C (en) * | 2005-11-26 | 2008-05-28 | 山西大学 | Microorganism polysaccharide and its preparation method and application |
| CN101524374B (en) * | 2008-03-04 | 2012-01-11 | 德阳市食用菌专家大院 | Auricularia Polytricha(Mont)Sacl fermentation product, oral preparation containing same, preparation method and application |
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| CN1302902A (en) | 2001-07-11 |
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