CN106136136A - A kind of method utilizing Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae production functional food - Google Patents
A kind of method utilizing Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae production functional food Download PDFInfo
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- CN106136136A CN106136136A CN201610544069.XA CN201610544069A CN106136136A CN 106136136 A CN106136136 A CN 106136136A CN 201610544069 A CN201610544069 A CN 201610544069A CN 106136136 A CN106136136 A CN 106136136A
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- monascus
- sato
- rhizoma dioscoreae
- functional food
- anka nakazawa
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
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Abstract
The invention discloses a kind of method utilizing Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae production functional food, relate to technical field of bioengineering.Described preparation method sequentially passes through test tube amplification culture, liquid submerged culture and seed tank amplification culture, solid fermentation is cultivated, dry and pulverising step prepares and has regulation blood fat, blood glucose and blood pressure, radioprotective and the functional food of suppression tumor.The present invention, with Rhizoma Dioscoreae and rice, Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers. etc. as solid matrix, with Monascus anka Nakazawa et sato for strain by solid fermentation, converts Rhizoma Dioscoreae Saponin, simultaneously synthesizing monascorubin and Monascus polysaccharides；In obtained product, saponin content 2～20mg/g butt matter, Monascus polysaccharides content 10～100mg/g butt matter, lovastatin content 0.5～10mg/g butt matter, content of monascus pigments 1～20mg/g butt matter, can be used for extracting Saponin, Monascus polysaccharides, lovastatin and monascorubin, produce treatment and reduce blood fat, blood glucose and blood pressure, radioprotective and the tablet of suppression tumor or capsule.
Description
Technical field
The present invention relates to technical field of bioengineering, be specifically related to one and utilize Monascus anka Nakazawa et sato to convert the Rhizoma Dioscoreae functional food of production
The method of product.
Background technology
Rhizoma Dioscoreae is the cultigen that can form underground meat tuber in potato preparatory course Wild yam, annual or perennial prehensile rattan
This plant, another name Rhizomadioscoreae, potato damage, RHIIZOMA DIOSCOREAE fr0m Henan of China etc..Rhizoma Dioscoreae is the highly efficient and productive industrial crops of a kind of dietotherapeutic, drying is used as medicine
For tonic invigorator, weakness, chronic enteritis, diabetes etc. there is auxiliary curative effect.Rhizoma Dioscoreae rich in proteins, aminoacid, starch etc.
The bioactive ingredients such as nutrient substance and polysaccharide, saponin, vitamin, allantoin, dopamine, Rhizoma Dioscoreae alkali, polyphenol oxidase.
Compendium of Material Medica Rhizoma Dioscoreae controls weakness, treats five kinds of strain and seven kinds of impairment, only lumbago, heat clearing away and restlessness relieving, reinforcing the heart QI-insufficiency, reach central hole, many accounts,
The effects such as bone and muscle strengthening, kidney tonifying gas, strengthening the spleen and stomach, antidiarrheal dysentery, the saliva that reduces phlegm, profit fur, " Records of Tradition Chinese and Western Medicine in Combination " is recorded Rhizoma Dioscoreae and is had
Only quench one's thirst (diabetes).Modern medicine study proves that Rhizoma Dioscoreae has regulation or enhancing human body immunity, reduces blood pressure and blood fat effect
And antitumor, antioxidation, defying age and mutation etc. act on.
The initial eating method of Rhizoma Dioscoreae be remove the peel, dry, long term storage after pulverizing, generally Rhizoma Dioscoreae powder is made respectively by steaming and decocting
Kind of food or Rhizoma Dioscoreae is directly stewed into soup eat.In the last few years, along with the progress of science and technology, people's exploitation to Rhizoma Dioscoreae
The most gradually to in-depth development, the report about Rhizoma Dioscoreae product R & D is a lot: Rhizoma Dioscoreae convection drying is made full powder, essence
Powder, directly pulls an oar Rhizoma Dioscoreae and makes series beverage, Rhizoma Dioscoreae starch is made ourishing rice flour, and Rhizoma Dioscoreae and pure milk, rice etc. is compound
It is fermented into yam Yogurt and yam wine etc..At present, mountain the effective elements of the medicine extracting directly is out developed into there is health care
Food be increasingly becoming Rhizoma Dioscoreae develop new approaches.But rarely report utilizes medical edible fungal to give birth to Rhizoma Dioscoreae
The research that thing converts.
Inventor, through further investigation and industrialized production test repeatedly, finds out industrially scalable conversion Rhizoma Dioscoreae raw
Produce functional food technology, the present invention no matter from the mode of production and the product of production of product, be all different from conventional patent and
The content of article report, a kind of method utilizing Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae production functional food.
Summary of the invention
, problem to be solved
For the above-mentioned problems in the prior art, the present invention provides one to utilize Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae and produces functional food
The method of product, its with making beating Rhizoma Dioscoreae and rice, Semen Tritici aestivi, Semen Maydis and Sorghum vulgare Pers. as solid matrix, pass through solid with Monascus anka Nakazawa et sato for strain
Fermentation, converts Rhizoma Dioscoreae, makes Monascus anka Nakazawa et sato mycelium and Rhizoma Dioscoreae interaction produce and have the functional of pharmacy function by fermentation
Food, technique is simple, and in obtained product, saponin content 2～20mg/g butt matter, Monascus polysaccharides content 10～100mg/
G butt matter, lovastatin content 0.5～10mg/g butt matter, content of monascus pigments 1～20mg/g butt matter.This product also may be used
With Saponin, monascorubin, the thus obtained product of polysaccharide, there is the crude drug identical with fermented product with extraction lovastatin further
Learn function.
, technical scheme
In order to solve the problems referred to above, the technical solution adopted in the present invention is as follows:
Described a kind of utilize Monascus anka Nakazawa et sato to convert the method that Rhizoma Dioscoreae produces functional food, described preparation method with making beating Rhizoma Dioscoreae,
Rice, Semen Tritici aestivi, Semen Maydis and Sorghum vulgare Pers. are main matrix, with Monascus anka Nakazawa et sato as starting strain, sequentially pass through test tube amplification culture, liquid shakes
Bottle is cultivated and the step such as seed tank amplification culture, solid fermentation cultivation；Described functional food can be again respectively through extracting system
Obtaining Saponin and lovastatin mixed extract, monascorubin yeast powder, Monascus polysaccharides extract, wherein Saponin and lovastatin carry
Taking Saponin and lovastatin content in thing and be respectively 25～60% and 30～70%, Monascus polysaccharides purity 20～60%, monascorubin is pure
Degree 30～60%.
Described a kind of Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food method, the Rhizoma Dioscoreae raw material that the method is used
Including: RHIIZOMA DIOSCOREAE fr0m Henan of China (Dioscorea oppositaThunb), mesa medicine (Dioscorea alataAnd mutation sole L.)
Sweet potato (Dioscorea alataL.f. flabella Makino.) and include dioscorea japonica (Dioscorea japonica
Thunb.), brown bud potato (Dioscorea persimilisPrain et Burk.), mountain potato (Dioscorea fordii
Prain et Burk.) or spit of fland Rhizoma Dioscoreae (Dioscorea quinquelabaSoil Rhizoma Dioscoreae, the Rhizoma Dioscoreae such as Thunb.)
(Dioscorea oppositaFoshou) or Fructus Citri Sarcodactylis Rhizoma Dioscoreae (Dioscorea oppositaIron-rod)；Used is red
Aspergillosis strain include monascus (Monascus anka) GIM3.592, red monascus (Monascus rubervan
Tieghem) GIM3.240, feathering monascus (Monascus pilosus) CGMCC 3.4653, Monascus fulginosus
(Monascus fuliginosus) CGMCC 3.2134, Florida monascus (Monascus floridanus) CGMCC
3.5843, Bark monascus (Monascus barkeri) CGMCC 3.4452, monascus aurantiacus (Monascus aurantiacus) CGMCC 3.4384, pale monascus (Monascus pallens) CGMCC 3.5844 or rust Monas cuspurpureus Went
Mould (Monascus rubiginosus) CGMCC 3.2844。
A kind of Monascus anka Nakazawa et sato converts the method that Rhizoma Dioscoreae produces functional food, carries out as steps described below:
A1 test tube amplification culture: Monascus anka Nakazawa et sato slant strains be inoculated in potato dextrose medium and cultivate, prepares red
Aspergillosis test tube slant strain；
A2 liquid submerged culture: the Monascus anka Nakazawa et sato test tube slant strain obtained by step A1 is inoculated into equipped with liquid submerged culture base
Shaking flask in, cultivate, prepare Monascus anka Nakazawa et sato liquid shaking bottle strain；
A3 seed tank amplification culture: be inoculated in seed tank culture base by the Monascus anka Nakazawa et sato liquid shaking bottle strain obtained by step A2
Row is cultivated, and makes Monascus anka Nakazawa et sato seed tank strain；
A4 solid fermentation is cultivated: is inoculated in solid medium by the Monascus anka Nakazawa et sato seed tank strain obtained by step A3, and mixes
Uniformly, carry out fermentation culture, prepare Monascus anka Nakazawa et sato solid fermentation thing；
A5 packs: Monascus anka Nakazawa et sato solid fermentation thing converts the functional food of Rhizoma Dioscoreae through drying, pulverize, pack prepared Monascus anka Nakazawa et sato.
Preferably, a kind of Monascus anka Nakazawa et sato converts the method that Rhizoma Dioscoreae produces functional food, carries out as steps described below:
B1 test tube amplification culture: slant strains in Monascus anka Nakazawa et sato test tube is cut into 3 × 3 mm fritter strains, inoculates a fritter to test tube
In slant medium, cultivating 4～15 days for 20～35 DEG C, prepare test tube slant strain, 4 DEG C, this test tube slant saves backup；
B2 liquid submerged culture: the test tube slant strain obtained by step B1 is cut into 3 × 3 mm fritter strains, picking 3～10
Block is inoculated in the 250mL triangular flask equipped with 20～150mL liquid submerged culture bases, triangular flask rotating speed be 50～200 turns/
Point, under conditions of temperature 20～35 DEG C, cultivate 18-86h, make liquid shaking bottle strain；
B3 seed tank amplification culture: the liquid shaking bottle strain obtained by step B2 is inoculated in seed tank amplification culture base, and
Described liquid shaking bottle strain and seed tank amplification culture base are the inoculum concentration inoculation of 1～20% by volume, are 20～35 in temperature
DEG C, speed of agitator is 50～160 revs/min, is 0.2 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
～under the conditions of the ventilation of 1.8:1, cultivate 18～96h, make Monascus anka Nakazawa et sato seed tank strain；
B4 solid fermentation is cultivated: accessed in solid fermentation culture medium by the Monascus anka Nakazawa et sato seed tank strain obtained by step B3, and institute
State the inoculum concentration inoculation that Monascus anka Nakazawa et sato seed tank strain and solid fermentation culture medium are 2～10% by weight, in temperature 20～35
DEG C, humidity 80% is fermented 5～20 days, and wherein every 3～6 days, stirring once, prepares solid fermentation thing；
B5 solid fermentation thing, after 80～120 DEG C dry 10～24 hours, is crushed to below 100 mesh, is packaged to be and can eat
Functional food；
In step A4, A5 or step B4, B5, functional food carries out the extracting method of lovastatin and Saponin and comprises the steps:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
60～the ethanol of 100% of 5～20 times of volumes of filament weight, repeatedly extracts 3～5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3～18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down containing Saponin and Lip river
Statin functional food, this food Saponin and lovastatin content are respectively 25～60% and 30～70%.
In step A4, A5 or step B4, B5, functional food carries out the extraction of Monascus polysaccharides and comprises the steps:
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds 5～20 times of volumes of mycelium weight
Distilled water, 80～100 DEG C are repeatedly extracted 3～5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, add residual volume 0.5～
95% ethanol of 1.5 times, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extraction raffinate volume 2.5～
95% ethanol of 3.5 times, crystallizes 5～18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides
Purity 20～60%.
In step A4, A5 or step B4, B5, functional food carries out the extracting method of monascorubin and comprises the steps:
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and the volume ratio of the 10-40 times of volume adding functional food weight is dense
Degree is 60-100% ethanol, and 60-90 DEG C is incubated 4-6 hour；Filtering, residue adds the volume of 10-40 times of volume of residue weight
Specific concentration is 60-100% ethanol, and 60-90 DEG C is incubated 4-6 hour, repeats 2-3 time；
E2 supernatant macroporous resin static adsorption 5-20 hour of 1/50-1/5 volume, 10-20 times of resin volume after absorption
Water washs, and installs in chromatographic column, and with the 20-80% ethanol desorbing of 10-20 times of resin volume, stripping liquid is incorporated in temperature 20-50
DEG C, it is concentrated in vacuo to the 1/100-1/50 of supernatant volume；
Preferably macroporous resin includes all low pole macroporous resins, such as AB-8 type, D-113 type, D-152 type, HD-2 type, HZD-
3 types, HZD-5 type etc.；
E3 concentrated solution is by regulation flow speed control air inlet temperature 160-190 DEG C, and air outlet temperature 100-120 DEG C carries out spray dried
Dry obtain monascorubin yeast powder.Monascorubin purity 30～60% in monascorubin yeast powder.
Preferably, liquid submerged culture base described in step B2 is sterilizing 10～60 min system under the conditions of 100～130 DEG C
, and containing wheat bran 5～20g, the raw material of the Rhizoma Dioscoreae 5～20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base.
Preferably, the base of seed tank amplification culture described in step B3 is sterilizing 10～60 min system under the conditions of 100～130 DEG C
, and containing wheat bran 5～20g, the raw material of the Rhizoma Dioscoreae 5～20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base.
Preferably, solid fermentation culture medium described in step B4 is sterilizing 90～360 min under the conditions of 100～130 DEG C
Prepare, and 10000 revs/min every kilogram homogenate Rhizoma Dioscoreae add add 200～400g rice, 100～300g Semen Tritici aestivi, 100～
The solid matrix raw materials such as 300g Semen Maydis and 700～300g Sorghum vulgare Pers..
Preferably, the raw material in described solid fermentation culture medium mixes with water by weight the ratio for 1:0.6～1.5.
, beneficial effect
Compared to prior art, the invention have the benefit that
(1) present invention is on the basis of drawing herb fermenting pharmaceutical technology, in conjunction with the advantage of modern solid-state fermentation, uses solid-state to send out
Ferment converts Rhizoma Dioscoreae, and during overcoming liquid fermentation, the suppression of Monascus anka Nakazawa et sato mycelial growth is made by substrate (active component of Rhizoma Dioscoreae)
With, simultaneously without liquid fermentation waste water and residue contamination；
(2) a kind of method utilizing Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae production functional food of the present invention, is not simple utilization
Then the active component of active component and Monascus anka Nakazawa et sato that process for separation and purification extracts Rhizoma Dioscoreae carries out compounding forming, but with Monascus anka Nakazawa et sato
For strain by solid fermentation, directly eat after converting Rhizoma Dioscoreae, both improve effect of Rhizoma Dioscoreae active component, enhanced again Monas cuspurpureus Went
Bacterium active component Monascus polysaccharides, monascorubin and the amount of lovastatin, can realize industrialization large-scale production；
(3) a kind of Monascus anka Nakazawa et sato of the present invention convert Rhizoma Dioscoreae produce functional food method, in its product saponin content 2～
20mg/g butt matter, lovastatin content 0.5～10mg/g butt matter, Monascus polysaccharides content 10～100mg/g butt matter, Monas cuspurpureus Went
Pigment content 1～20mg/g butt matter.There is the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.From this product
The lovastatin extracted in product and Saponin, monascorubin, Monascus polysaccharides have regulation blood fat, blood glucose and blood pressure, radioprotective equally
With suppression tumor function.
Accompanying drawing explanation
Fig. 1 is the preparation flow of a kind of method utilizing Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae production functional food of the present invention
Schematic diagram.
Detailed description of the invention
The mensuration of described saponin content use spectrophotography (Tang Zhonghou, Shi Xinmin, Sun Jian, Li Hongmin, Zhang Aijun:
Total Saponins of Chinese Yam assay and Genotypic Difference thereof. Agriculture in Jiangxi journal 2011,23 (2): 50～52).
The mensuration of described content of monascus pigments uses " National Standard of the People's Republic of China's food additive Monas cuspurpureus Went
GB4926-85 " method mensuration.
Described lovastatin content uses dual-wavelength ultraviolet spectrophotometry to measure: accurately weigh sample 1g 30mL75%
Ethanol repeats to extract 3 times, and extracting solution merges.6000rpm is centrifuged 10 minutes, and supernatant flows through diameter 9 with 0.5 mL/min flow velocity
The chromatographic column of mm, chromatographic column filler is the 10 grams 100 DEG C activation neutral alumina of 30 minutes, overanxious liquid respectively at 246nm and
Absorbance A is measured at 254nm246And A254, according to absorbance difference Asample=A246-A254It is that him is cut down in 1mg/mL standard Lip river with concentration
Absorbance difference Astandard=A246-A254 in spit of fland, according to following equation calculating lovastatin content p:
Here n: for extension rate.
Described Monascus polysaccharides content deducts reducing sugar equal to total sugar, and the mensuration of total sugar uses sulfuric acid-phynol method (Zhaoyang
Nanmu, Chang Jidong. Phenol sulfuric acid procedure and indirect iodometric processes measure Monascus polysaccharides comparision contents [J]. edible fungi, 2007, (3): 58
～61.), content of reducing sugar measures employing 3,5-dinitrosalicylic acid system (Miller GL. Use of
dinitrosalicylic acid reagent for determination of reducing sugars.
Analytical Chemistry. 1959,1:426 ~ 428.).
Described Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the evaluation methodology employing high blood lipid model Mus of functional food regulation blood fat
Evaluate (Cao Min etc.: sanchi flower total saponine hypotensive effect is studied. the bright traditional Chinese medical science, and 2012,27 (7): 1314-1315).Concrete operations
For: after 40 mices adapt to feed 5d with normal feedstuff, take tail hematometry Triglycerides in Serum content, with triglyceride
Level is randomly divided into 4 groups: Normal group, hyperlipidemia matched group, low dosage experimental group, high dose experimental group.Normal control
Group mice feed normal feedstuff (12% casein, 60.98% corn starch, 15% sucrose, 7% Semen Maydis oil, 1% vitamin Icing Sugar, 4%
Mineral salt, 0.02% cod-liver oil)；(2% gallbladder is solid for 15.75% lard stearin, 7.79% sucrose for hyperlipidemia model control group fed high lipid food
Alcohol, 0.5% cholic acid, 73.96% normal feedstuff；Low dosage and high dose group mice feed per kilogram high lipid food and contain 10 grams and 20
Gram Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae functional food.Each group mice the most freely ingests and drinks water, animal housing's temperature 18～22 DEG C.Continuously
After being administered surrounding, tail vein takes blood, analyzes serum triglyceride, T-CHOL, high density lipoprotein and low density lipoprotein
Protein content
Described Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the evaluation methodology employing barbital of functional food regulation blood fat and ligature left kidney
(yellow will is new: Herba Visci, the hypotensive activity of the Cortex Eucommiae and acute toxicity in the Hypertensive Rats evaluation of tremulous pulse combined induction
Experimentation. research and development of natural products, 2003,15 (3): 245-248).Hypertension model Mus is divided into three groups of blanks
The Monascus anka Nakazawa et sato that group, low dose group and high dose group feed normal feedstuff respectively, per kilogram contains 10 grams converts the functional food of Rhizoma Dioscoreae
The Monascus anka Nakazawa et sato that the normal feedstuff of product and per kilogram contain 20 grams converts the normal feedstuff of Rhizoma Dioscoreae functional food.Successive administration 14 days
Blood pressure after measuring before being administered and being administered.
Described Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the evaluation methodology employing alloxan induction of functional food regulation blood glucose
Diabetic rat model evaluation.Induction reference literature (the Effect of FeSO such as Zhicai Zhang of model mouse4
treatment on glucose metabolism in diabetic rats. Biometals (2008) 21:685–
691) carry out.Hyperglycemia model Mus is divided into three groups of blank groups, low dose group and high dose group to feed normal feedstuff, every respectively
Kilogram convert the normal feedstuff of Rhizoma Dioscoreae functional food containing the Monascus anka Nakazawa et sato of 10 grams and Monascus anka Nakazawa et sato that per kilogram contains 20 grams converts
The normal feedstuff of Rhizoma Dioscoreae functional food.Successive administration measures the blood glucose value before being administered and after administration for 28 days.
Described Monascus anka Nakazawa et sato converts the Rhizoma Dioscoreae production radiation-resistant evaluation methodology of functional food and uses radiation irradiation in rats to enter
Row evaluation (a diplodocus man of virtue and ability. the effect study of Lac regis apis Antiradiation injury. Heilungkiang medical science, 2012,36 (10): 724-726).30
SD male rat feeds normal feedstuff respectively, converts the normal feedstuff 15 days of Rhizoma Dioscoreae functional food containing 1% and 2% Monascus anka Nakazawa et sato
After, rat is feeding60Co gamma-rays carries out total irradiation, away from radiation source 1.0 m close rate 2.0 Gy, accumulated dose 200rad.Irradiate
Measuring body weight, mouse mortality rate after 10 days, tail vein takes haemanalysis quantity of leucocyte.
Described Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the evaluation methodology employing suppression Primary Hepatic of functional food suppression tumor
Cancerous cell Hepg2 growth effect (Zhouning County, Chen Jiangtao, Yu Wenyan. Water Extract of Ficus Carica to suppression tumor cell proliferation
The preliminary study of effect. Xinjiang Medicine University's journal. 2016,39 (1): 42-47).Hepg2 groups of cells complete culture solution
Adjusting cell concentration is 2 × 104Individual/mL, is inoculated in 96 orifice plates, and every hole cumulative volume is 200 μ L, and overnight incubation makes cell attachment,
Next day respectively with the Monascus anka Nakazawa et sato of various dose convert the ethanol extract of Rhizoma Dioscoreae functional food and aqueous extract (substrate: water (or
Dehydrated alcohol)=1:10,70 DEG C are extracted 4 hours) and processing cell 24h respectively, matched group adds PBS and processes 24h, and each mensuration connects
Plant 3 multiple holes, be placed in 37 DEG C, 5%CO2After incubator processes 24, every hole adds 5mg/mL tetrazolium bromide (MTT) reagent 20 μ L, continues training
Support 4h, then add dimethyl sulfoxide (DMSO) 150 μ L, vibration mixing 10min, with microplate reader (λ=490nm) measure OD value (OD value and
Viable count is directly proportional), cell inhibitory rate=1-(dosing group OD value/matched group OD value), experiment is repeated 3 times.
Below in conjunction with specific embodiment, the present invention is further described below.
Embodiment 1
Utilize Monascus anka Nakazawa et sato to convert Rhizoma Dioscoreae to produce the method for functional food and specifically include following steps as it is shown in figure 1, a kind of:
(1) test tube amplification culture: by monascus (Monascus anka) to be cut into 3 × 3 mm little for GIM3.592 test tube slant strain
Truffles kind, inoculates a fritter in test tube slant culture medium, cultivates 15 days for 20 DEG C, prepare test tube slant strain, this test tube slant 4
DEG C save backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 10 pieces
Being inoculated in the 250mL triangular flask equipped with 150mL liquid submerged culture base, triangular flask is 50 revs/min at rotating speed, temperature 35 DEG C
Under the conditions of, 18h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 10 under the conditions of 130 DEG C
Min prepares, and containing wheat bran 5g, the raw material of the Rhizoma Dioscoreae 20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 1% by volume, it is 35 DEG C in temperature, stirs
Mixing rotating speed is 160 revs/min, is the ventilation of 0.2:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 96h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 130 DEG C
10 min prepare, and containing wheat bran 5g, the raw material of the Rhizoma Dioscoreae 20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Monascus anka Nakazawa et sato seed tank strain access equipped with RHIIZOMA DIOSCOREAE fr0m Henan of China (Dioscorea oppositaThunb) in solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain is with solid fermentation culture medium by weight
Inoculating than the inoculum concentration being 10%, temperature 20 DEG C, humidity 80% is fermented 40 days, and wherein every 6 days, stirring once, prepares solid
Fermented product；Described solid fermentation culture medium is that sterilizing 360 min prepares under the conditions of 130 DEG C, and solid medium is by every kilogram
The Rhizoma Dioscoreae of 10000 revs/min of homogenate adds the solid matrix raw materials such as 200g rice, 100g Semen Tritici aestivi, 100g Semen Maydis and 700g Sorghum vulgare Pers.
Prepare；Raw material in described solid fermentation culture medium mixes with water by weight the ratio for 1:1.1；
(5) by the solid fermentation thing obtained by step (4) after 120 DEG C dry 10 hours, it is crushed to below 100 mesh, packaging
Obtain edible functional food；Described functional food contains the Saponin of 2mg/g butt matter, 10mg/g butt matter
Monascus polysaccharides, the monascorubin of 1mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, resists
The effects such as radiation and suppression tumor；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain containing Saponin and the product of lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 60% of 5 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and lovastatin
Functional food, this food Saponin and lovastatin content are respectively 25% and 70%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 5 times of volumes of mycelium weight
Water, 80 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.5 times
95% ethanol, under the conditions of 4 DEG C crystallize 5 hours, filter, filtrate continuously add extract raffinate volume 2.5 times 95% ethanol,
Crystallizing 5 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 20%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 10 times of volumes of functional food weight
Being 60% ethanol, 60 DEG C are incubated 4 hours；Filtering, the volume by volume concentration of 10 times of volumes that residue adds residue weight is 60% wine
Essence, 60 DEG C are incubated 4 hours, are repeated 2 times；
The E2 supernatant macroporous resin AB-8 type static adsorption 5 hours of 1/50 volume, adsorbs the washing of rear 10 times of resin volumes
Washing, install in chromatographic column, with 20% ethanol desorbing of 10 times of resin volumes, stripping liquid is incorporated in temperature 20 DEG C, is concentrated in vacuo supreme
The 1/100 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 160 DEG C, and air outlet temperature 100 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 30% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 2
A kind of Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the method for functional food and specifically include following steps:
(1) test tube amplification culture: by red monascus (Monascus rubervanTieghem) GIM3.240 test tube slant
Strain is cut into 3 × 3 mm fritter strains, inoculates a fritter in test tube slant culture medium, cultivates 4 days for 35 DEG C, prepare test tube slant
Strain, 4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 3 pieces
Being inoculated in the 250mL triangular flask equipped with 20mL liquid submerged culture base, triangular flask is 200 revs/min at rotating speed, temperature 20 DEG C
Under the conditions of, 86h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 60 under the conditions of 100 DEG C
Min prepares, and containing bran 20g, the raw material of the Rhizoma Dioscoreae 5g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 20% by volume, it is 20 DEG C in temperature, stirs
Mixing rotating speed is 50 revs/min, is 0.2～1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of ventilation, cultivate 18h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is under the conditions of 100 DEG C
Sterilizing 60min prepares, and containing wheat bran 20g in every liter of liquid submerged culture base, the Rhizoma Dioscoreae 5g's of 10000 revs/min of homogenate is former
Material；
(4) solid fermentation cultivate: by obtained by step (3) Monascus anka Nakazawa et sato seed tank strain access equipped with mesa medicine (Dioscorea alataL.) in solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain is 2% with solid fermentation culture medium by weight
Inoculum concentration inoculation, temperature 35 DEG C, humidity 80% is fermented 20 days, wherein every 3 days, stirring once, prepare solid fermentation thing；
Described solid fermentation culture medium is that sterilizing 90min prepares under the conditions of 100 DEG C, and solid medium is by 10000 revs/min every kilogram
The Rhizoma Dioscoreae of clock homogenate adds the solid matrix raw materials such as 400g rice, 300g Semen Tritici aestivi, 300g Semen Maydis and 300g Sorghum vulgare Pers. and prepares；Described solid
Raw material in body fermentation medium mixes with water by weight the ratio for 1:1.5；
(5) by the solid fermentation thing obtained by step (4) after 80 DEG C dry 10 hours, it is crushed to below 100 mesh, packs
To edible functional food；Described functional food contains the Saponin of 20mg/g butt matter, 100mg/g butt matter
Monascus polysaccharides, the monascorubin of 20mg/g butt matter, animal experiment proves, this product have regulation blood fat, blood glucose and blood pressure,
The effects such as radioprotective and suppression tumor；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain containing Saponin and the product of lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 100% of 20 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down him containing Saponin and Lip river
Spit of fland functional food, this food Saponin and lovastatin content are respectively 60% and 30%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 20 times of volumes of mycelium weight
Distilled water, 100 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.5 times
95% ethanol, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extract raffinate volume 3.5 times 95%
Ethanol, crystallizes 18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 60%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 40 times of volumes of functional food weight
Being 100% ethanol, 90 DEG C are incubated 6 hours；Filtering, the volume by volume concentration of 40 times of volumes that residue adds residue weight is 100%
Ethanol, 90 DEG C are incubated 6 hours, are repeated 3 times；
The E2 supernatant macroporous resin D-113 type static adsorption 20 hours of 1/5 volume, adsorbs the washing of rear 20 times of resin volumes
Washing, install in chromatographic column, with 80% ethanol desorbing of 20 times of resin volumes, stripping liquid is incorporated in temperature 50 C, is concentrated in vacuo supreme
The 1/50 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 190 DEG C, and air outlet temperature 120 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 60% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 3
A kind of Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the method for functional food and specifically include following steps:
(1) test tube amplification culture: by feathering monascus (Monascus pilosus) CGMCC 3.4653 test tube slant strain cuts
Become 3 × 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 10 days for 28 DEG C, prepare test tube slant strain,
4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 7 pieces
Being inoculated in the 250mL triangular flask equipped with 80mL liquid submerged culture base, triangular flask is 120 revs/min at rotating speed, temperature 28 DEG C
Under the conditions of, 57h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 35 under the conditions of 120 DEG C
Min prepares, and containing wheat bran 13g, the raw material of the Rhizoma Dioscoreae 13g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 10% by volume, it is 28 DEG C in temperature, stirs
Mixing rotating speed is 110 revs/min, is the ventilation of 1:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of, cultivate 57h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 120 DEG C
35min prepares, and containing wheat bran 13g, the raw material of the Rhizoma Dioscoreae 13g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Monascus anka Nakazawa et sato seed tank strain access equipped with sole sweet potato (Dioscorea alataL.f. flabella Makino.) in Rhizoma Dioscoreae solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain and solid
Fermentation medium is the inoculum concentration inoculation of 6% by weight, and temperature 28 DEG C, humidity 80% is fermented 30 days, wherein every 4.5 days,
Stirring once, prepares solid fermentation thing；Described solid fermentation culture medium is that sterilizing 260 min prepares under the conditions of 120 DEG C, and solid
It is high that body culture medium adds 300g rice, 200g Semen Tritici aestivi, 200g Semen Maydis and 500g by the Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate
The solid matrix raw materials such as fine strain of millet prepare；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:0.6；
(5) solid fermentation thing is after 100 DEG C dry 17 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 11mg/g butt matter, and the Monascus polysaccharides of 54mg/g butt matter, 11mg/g are dry
The monascorubin of substrate, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc.
Effect；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain containing Saponin and the product of lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 80% of 12 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 11 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down him containing Saponin and Lip river
Spit of fland functional food, this food Saponin and lovastatin content are respectively 43% and 50%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 13 times of volumes of mycelium weight
Distilled water, 90 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 12 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3 times, in 4
Crystallizing 12 hours under the conditions of DEG C, then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 41%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 30 times of volumes of functional food weight
Being 90% ethanol, 70 DEG C are incubated 5 hours；Filtering, the volume by volume concentration of 20 times of volumes that residue adds residue weight is 90% wine
Essence, 70 DEG C are incubated 5 hours, repeat 2-3 time；
The E2 supernatant macroporous resin D-152 type static adsorption 8 hours of 1/40 volume, adsorbs the washing of rear 12 times of resin volumes
Washing, install in chromatographic column, with 40% ethanol desorbing of 12 times of resin volumes, stripping liquid is incorporated in temperature 30 DEG C, is concentrated in vacuo supreme
The 1/90 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 170 DEG C, and air outlet temperature 110 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 54% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 4
A kind of Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Monascus fulginosus (Monascus fuliginosus) CGMCC 3.2134 test tube slant bacterium
Kind it is cut into 3 × 3 mm fritter strains, inoculates a fritter in test tube slant culture medium, cultivate 12 days for 23 DEG C, prepare test tube slant
Strain, 4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 8 pieces
Being inoculated in the 250mL triangular flask equipped with 30mL liquid submerged culture base, triangular flask is 75 revs/min at rotating speed, temperature 20～35
Under conditions of DEG C, 30h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing under the conditions of 100 DEG C
55 min prepare, and containing wheat bran 8g, the raw material of the Rhizoma Dioscoreae 12g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 4% by volume, it is 25 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 0.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 35h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 100 DEG C
55 min prepare, and containing wheat bran 8g, the raw material of the Rhizoma Dioscoreae 15g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Monascus anka Nakazawa et sato seed tank strain access equipped with dioscorea japonica (Dioscorea japonicaThunb.) in solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain is with solid fermentation culture medium by weight
Inoculating than the inoculum concentration being 4%, temperature 22 DEG C, humidity 80% is fermented 25 days, and wherein every 3 days, stirring once, send out by prepared solid
Ferment thing；Described solid fermentation culture medium is that sterilizing 100 min prepares under the conditions of 100 DEG C, and solid medium is by every kilogram
The Rhizoma Dioscoreae of 10000 revs/min of homogenate adds the solid matrix raw materials such as 250g rice, 150g Semen Tritici aestivi, 150g Semen Maydis and 600g Sorghum vulgare Pers.
Prepare；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:1.0；
(5) by the solid fermentation thing obtained by step (4) after 90 DEG C dry 22 hours, it is crushed to below 100 mesh, packs
To edible functional food；Described functional food contains the Saponin of 18mg/g butt matter, 90mg/g butt matter
Monascus polysaccharides, the monascorubin of 5mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, resists
The effects such as radiation and suppression tumor；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain containing Saponin and the product of lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 70% of 7 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 6 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing Saponin and lovastatin
Functional food, this food Saponin and lovastatin content are respectively 31% and 41%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 7 times of volumes of mycelium weight
Water, 85 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.7 times
95% ethanol, under the conditions of 4 DEG C crystallize 7 hours, filter, filtrate continuously add extract raffinate volume 2.8 times 95% ethanol,
Crystallizing 7 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 31%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 30 times of volumes of functional food weight
Being 70% ethanol, 80 DEG C are incubated 4 hours；Filtering, the volume by volume concentration of 30 times of volumes that residue adds residue weight is 70% wine
Essence, 80 DEG C are incubated 4 hours, are repeated 3 times；
The E2 supernatant macroporous resin HD-2 type static adsorption 12 hours of 1/30 volume, adsorbs the washing of rear 15 times of resin volumes
Washing, install in chromatographic column, with 70% ethanol desorbing of 15 times of resin volumes, stripping liquid is incorporated in temperature 40 DEG C, is concentrated in vacuo supreme
The 1/80 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 180 DEG C, and air outlet temperature 100 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 52% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 5
A kind of Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Florida monascus (Monascus floridanus) CGMCC 3.5843 test tube is oblique
Face strain is cut into 3 × 3 mm fritter strains, inoculates a fritter in test tube slant culture medium, cultivates 6 days for 32 DEG C, prepare test tube oblique
Face strain, 4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 40mL liquid submerged culture base, triangular flask is 160 revs/min at rotating speed, temperature 30 DEG C
Under the conditions of, 36h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 28 under the conditions of 128 DEG C
Min prepares, and containing wheat bran 6g, the raw material of the Rhizoma Dioscoreae 20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 30 DEG C in temperature, stirs
Mixing rotating speed is 160 revs/min, is the ventilation of 0.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 68h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 128 DEG C
28 min prepare, and containing wheat bran 15g, the raw material of the Rhizoma Dioscoreae 7g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Monascus anka Nakazawa et sato seed tank strain access equipped with brown bud potato (Dioscorea persimilisPrain et Burk.) in Rhizoma Dioscoreae solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain is sent out with solid
Ferment culture medium is the inoculum concentration inoculation of 6% by weight, and temperature 30 DEG C, humidity 80% is fermented 28 days, wherein every 5 days, and stirring
Once, solid fermentation thing is prepared；Described solid fermentation culture medium is that sterilizing 320 min prepares under the conditions of 128 DEG C, and solid training
Support base and add 350g rice, 250g Semen Tritici aestivi, 250g Semen Maydis and 400g Sorghum vulgare Pers. etc. by the Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate
Solid matrix raw material prepares；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:0.6；
(5) solid fermentation thing is after 90 DEG C dry 18 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 4mg/g butt matter, and the Monascus polysaccharides of 24mg/g butt matter, 16mg/g are dry
The monascorubin of substrate, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc.
Effect；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain containing Saponin and the product of lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 90% of 17 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 15 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down him containing Saponin and Lip river
Spit of fland functional food, this food Saponin and lovastatin content are respectively 25% and 61%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 18 times of volumes of mycelium weight
Distilled water, 95 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.4 times
95% ethanol, under the conditions of 4 DEG C crystallize 16 hours, filter, filtrate continuously add extract raffinate volume 3.2 times 95% second
Alcohol, crystallizes 16 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 52%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 15 times of volumes of functional food weight
Being 85% ethanol, 75 DEG C are incubated 6 hours；Filtering, the volume by volume concentration of 15 times of volumes that residue adds residue weight is 85% wine
Essence, 75 DEG C are incubated 6 hours, are repeated 2 times；
E2 supernatant macroporous resin HZD-3 type static adsorption 5-20 hour of 1/10 volume, adsorbs rear 18 times of resin volumes
Water washs, and installs in chromatographic column, and with 75% ethanol desorbing of 18 times of resin volumes, stripping liquid is incorporated in temperature 50 C, is concentrated in vacuo
To supernatant volume 1/40；
E3 concentrated solution is by regulation flow speed control air inlet temperature 165 DEG C, and air outlet temperature 118 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 36% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 6
A kind of Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the method for functional food and specifically include following steps:
(1) test tube amplification culture: by Bark monascus (Monascus barkeri) CGMCC 3.4452 test tube slant strain cuts
Become 3 × 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 5 days for 32 DEG C, prepare test tube slant strain,
4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 20mL liquid submerged culture base, triangular flask is 170 revs/min at rotating speed, temperature 25 DEG C
Under the conditions of, 60h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 50 under the conditions of 110 DEG C
Min prepares, and containing wheat bran 16g, the raw material of the Rhizoma Dioscoreae 8g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 25 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 1.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 29h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 110 DEG C
50 min prepare, and containing wheat bran 14g, the raw material of the Rhizoma Dioscoreae 10g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Monascus anka Nakazawa et sato seed tank strain access equipped with spit of fland Rhizoma Dioscoreae (Dioscorea quinquelabaThunb.) in solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain is pressed with solid fermentation culture medium
Weight ratio is the inoculum concentration inoculation of 6%, and temperature 25 DEG C, humidity 80% is fermented 26 days, and wherein every 5 days, stirring once, prepares solid
Body fermented product；Described solid fermentation culture medium is that sterilizing 250 min prepares under the conditions of 110 DEG C, and solid medium is by every thousand
It is former that the Rhizoma Dioscoreae of grams of 10000 revs/min homogenate adds the solid matrixs such as 400g rice, 150g Semen Tritici aestivi, 150g Semen Maydis and 500g Sorghum vulgare Pers.
Material prepares；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:0.8；
(5) by the solid fermentation thing obtained by step (4) after 110 DEG C dry 16 hours, it is crushed to below 100 mesh, packaging
Obtain edible functional food；Described functional food contains the Saponin of 6mg/g butt matter, 56mg/g butt matter
Monascus polysaccharides, the monascorubin of 18mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, resists
The effects such as radiation and suppression tumor；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain containing Saponin and the product of lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 63% of 11 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 12 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down him containing Saponin and Lip river
Spit of fland functional food, this food Saponin and lovastatin content are respectively 55% and 37%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 13 times of volumes of mycelium weight
Distilled water, 90 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 13 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3.0 times,
Crystallizing 13 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 45%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 40 times of volumes of functional food weight
Being 60% ethanol, 90 DEG C are incubated 4 hours；Filtering, the volume by volume concentration of 40 times of volumes that residue adds residue weight is 60% wine
Essence, 90 DEG C are incubated 4 hours, are repeated 3 times；
The E2 supernatant macroporous resin HZD-5 type static adsorption 5 hours of 1/5 volume, adsorbs the washing of rear 20 times of resin volumes
Washing, install in chromatographic column, with 80% ethanol desorbing of 20 times of resin volumes, stripping liquid is incorporated in temperature 50 C, is concentrated in vacuo supreme
The 1/100 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 190 DEG C, and air outlet temperature 100 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 60% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 7
A kind of Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the method for functional food and specifically include following steps:
(1) test tube amplification culture: by monascus aurantiacus (Monascus aurantiacus) CGMCC 3.4384 test tube slant bacterium
Kind it is cut into 3 × 3 mm fritter strains, inoculates a fritter in test tube slant culture medium, cultivate 13 days for 23 DEG C, prepare test tube slant
Strain, 4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 10 pieces
Being inoculated in the 250mL triangular flask equipped with 130mL liquid submerged culture base, triangular flask is 150 revs/min at rotating speed, temperature 23 DEG C
Under conditions of, 36h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 53 under the conditions of 115 DEG C
Min prepares, and containing wheat bran 9g, the raw material of the Rhizoma Dioscoreae 20g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 21 DEG C in temperature, stirs
Mixing rotating speed is 120 revs/min, is the ventilation of 0.2:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 81h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 115 DEG C
53 min prepare, and containing wheat bran 19g, the raw material of the Rhizoma Dioscoreae 18g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation is cultivated: access the Monascus anka Nakazawa et sato seed tank strain obtained by step (3) equipped with Rhizoma Dioscoreae
(Dioscorea oppositaFoshou) in solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain and solid fermentation
Culture medium is the inoculum concentration inoculation of 4% by weight, and temperature 21 DEG C, humidity 80% is fermented 34 days, wherein every 6 days, and stirring one
Secondary, prepare solid fermentation thing；Described solid fermentation culture medium is that sterilizing 250 min prepares under the conditions of 110 DEG C, and solid culture
It is solid that base adds 400g rice, 150g Semen Tritici aestivi, 250g Semen Maydis and 400g Sorghum vulgare Pers. etc. by the Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate
Body base starting material prepares；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:1.0；
(5) solid fermentation thing is after 115 DEG C dry 16 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 20mg/g butt matter, the Monascus polysaccharides of 100mg/g butt matter, 1mg/g
The monascorubin of butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor
Etc. effect；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain containing Saponin and the product of lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 75% of 9 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 10 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down him containing Saponin and Lip river
Spit of fland functional food, this food Saponin and lovastatin content are respectively 38% and 45%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 10 times of volumes of mycelium weight
Distilled water, 88 DEG C are repeatedly extracted 5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.8 times
95% ethanol, under the conditions of 4 DEG C crystallize 10 hours, filter, filtrate continuously add extract raffinate volume 2.9 times 95% second
Alcohol, crystallizes 10 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 38%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 10 times of volumes of functional food weight
Being 100% ethanol, 60 DEG C are incubated 6 hours；Filtering, the volume by volume concentration of 10 times of volumes that residue adds residue weight is 100%
Ethanol, 60 DEG C are incubated 6 hours, are repeated 2 times；
The E2 supernatant macroporous resin D-152 type static adsorption 20 hours of 1/50-1/5 volume, adsorbs rear 16 times of resin volumes
Water washing, install in chromatographic column, with 60% ethanol desorbing of 16 times of resin volumes, stripping liquid is incorporated in temperature 45 C, and vacuum is dense
It is reduced to the 1/100 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 170 DEG C, and air outlet temperature 110 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 42% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 8
A kind of Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the method for functional food and specifically include following steps:
(1) test tube amplification culture: by pale monascus (Monascus pallens) CGMCC 3.5844 test tube slant strain cuts
Become 3 × 3 mm fritter strains, inoculate a fritter in test tube slant culture medium, cultivate 10 days for 29 DEG C, prepare test tube slant strain,
4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 8 pieces
Being inoculated in the 250mL triangular flask equipped with 60mL liquid submerged culture base, triangular flask is 160 revs/min at rotating speed, temperature 33 DEG C
Under the conditions of, 80h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 30 under the conditions of 120 DEG C
Min prepares, and containing wheat bran 19g, the raw material of the Rhizoma Dioscoreae 19g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 18% by volume, it is 33 DEG C in temperature, stirs
Mixing rotating speed is 140 revs/min, is the ventilation of 1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of amount, cultivate 90h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 120 DEG C
30 min prepare, and containing wheat bran 18g, the raw material of the Rhizoma Dioscoreae 18g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation is cultivated: access the Monascus anka Nakazawa et sato seed tank strain obtained by step (3) equipped with Fructus Citri Sarcodactylis Rhizoma Dioscoreae
(Dioscorea oppositaIron-rod) in solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain is sent out with solid
Ferment culture medium is the inoculum concentration inoculation of 9% by weight, and temperature 33 DEG C, humidity 80% is fermented 37 days, wherein every 6 days, and stirring
Once, solid fermentation thing is prepared；Described solid fermentation culture medium is that sterilizing 350 min prepares under the conditions of 105 DEG C, and solid training
Support base and add 280g rice, 220g Semen Tritici aestivi, 270g Semen Maydis and 380g Sorghum vulgare Pers. etc. by the Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate
Solid matrix raw material prepares；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:1.4；
(5) solid fermentation thing is after 85 DEG C dry 23 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 18mg/g butt matter, and the Monascus polysaccharides of 94mg/g butt matter, 3mg/g are dry
The monascorubin of substrate, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc.
Effect；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain containing Saponin and the product of lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 97% of 16 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 15 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down him containing Saponin and Lip river
Spit of fland functional food, this food Saponin and lovastatin content are respectively 26% and 67%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 18 times of volumes of mycelium weight
Distilled water, 95 DEG C are repeatedly extracted 3 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.4 times
95% ethanol, under the conditions of 4 DEG C crystallize 16 hours, filter, filtrate continuously add extract raffinate volume 3.2 times 95% second
Alcohol, crystallizes 6 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 57%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 25 times of volumes of functional food weight
Being 65% ethanol, 82 DEG C are incubated 5 hours；Filtering, the volume by volume concentration of 25 times of volumes that residue adds residue weight is 65% wine
Essence, 82 DEG C are incubated 5 hours, are repeated 2 times；
E2 supernatant macroporous resin HD-2 type static adsorption 5-20 hour of 1/8 volume, adsorbs the water of rear 16 times of resin volumes
Washing, installs in chromatographic column, and with 48% ethanol desorbing of 16 times of resin volumes, stripping liquid is incorporated in temperature 36 DEG C, is concentrated in vacuo to
The 1/70 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 180 DEG C, and air outlet temperature 118 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 51% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Embodiment 9
A kind of Monascus anka Nakazawa et sato converts Rhizoma Dioscoreae and produces the method for functional food and specifically include following steps:
(1) test tube amplification culture: by rust monascus (Monascus rubiginosus) CGMCC 3.2844 test tube slant
Strain is cut into 3 × 3 mm fritter strains, inoculates a fritter in test tube slant culture medium, cultivates 6 days for 24 DEG C, prepare test tube slant
Strain, 4 DEG C, this test tube slant saves backup；
(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces
Being inoculated in the 250mL triangular flask equipped with 24mL liquid submerged culture base, triangular flask is 58 revs/min at rotating speed, temperature 24 DEG C
Under the conditions of, 20h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 59 under the conditions of 100 DEG C
Min prepares, and containing wheat bran 5g, the raw material of the Rhizoma Dioscoreae 5g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base,
And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 3% by volume, it is 24 DEG C in temperature, stirs
Mixing rotating speed is 58 revs/min, is the ventilation of 1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
Under the conditions of, cultivate 25h, make Monascus anka Nakazawa et sato seed tank strain；Described seed tank amplification culture base is sterilizing 59 under the conditions of 100 DEG C
Min prepares, and containing wheat bran 5g, the raw material of the Rhizoma Dioscoreae 6g of 10000 revs/min of homogenate in every liter of liquid submerged culture base；
(4) solid fermentation cultivate: by obtained by step (3) Monascus anka Nakazawa et sato seed tank strain access equipped with mountain potato (Dioscorea fordiiPrain et Burk.) in Rhizoma Dioscoreae solid fermentation culture medium, and described Monascus anka Nakazawa et sato seed tank strain is trained with solid fermentation
Foster base be by weight 3% inoculum concentration inoculation, temperature 24 DEG C, humidity 80% is fermented 23 days, wherein every 3 days, stirring once,
Prepare solid fermentation thing；Described solid fermentation culture medium is that sterilizing 150 min prepares under the conditions of 100 DEG C, and solid medium
The solids such as 360g rice, 240g Semen Tritici aestivi, 160g Semen Maydis and 540g Sorghum vulgare Pers. are added by the Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate
Base starting material prepares；Raw material in described solid fermentation culture medium mixes with water by weight the ratio of 1:1.3；
(5) solid fermentation thing is after 110 DEG C dry 13 hours, is crushed to below 100 mesh, is packaged to be edible function
Property food；Described functional food contains the Saponin of 2mg/g butt matter, and the Monascus polysaccharides of 10mg/g butt matter, 20mg/g are dry
The monascorubin of substrate, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc.
Effect；
(6) extract separate: by obtained by step (4) or (5) Monascus anka Nakazawa et sato convert Rhizoma Dioscoreae produce functional food respectively through
Extract, respectively obtain the product containing Saponin and lovastatin, monascorubin yeast powder and the product of Monascus polysaccharides.
It should be noted that and include walking as follows from the method for the product containing Saponin and lovastatin described in step (6)
Rapid:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
The ethanol of the 81% of 15 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 14 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down him containing Saponin and Lip river
Spit of fland functional food, this food Saponin and lovastatin content are respectively 57% and 36%.
In the present embodiment, comprise the steps: from the method containing Monascus polysaccharides product described in step (6)
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 13 times of volumes of mycelium weight
Distilled water, 92 DEG C are repeatedly extracted 4 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times
95% ethanol, crystallizes 12 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3.1 times,
Crystallizing 12 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides purity 42%；
In the present embodiment, comprise the steps: from the method containing monascorubin yeast powder described in step (6)
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and adds the volume by volume concentration of 28 times of volumes of functional food weight
Being 80% ethanol, 76 DEG C are incubated 6 hours；Filtering, the volume by volume concentration of 28 times of volumes that residue adds residue weight is 80% wine
Essence, 76 DEG C are incubated 6 hours, are repeated 2 times；
E2 supernatant macroporous resin HZD-3 type static adsorption 5-20 hour of 1/5 volume, adsorbs the water of rear 17 times of resin volumes
Washing, installs in chromatographic column, and with 30% ethanol desorbing of 17 times of resin volumes, stripping liquid is incorporated in temperature 21 DEG C, is concentrated in vacuo to
The 1/50 of supernatant volume；
E3 concentrated solution is by regulation flow speed control air inlet temperature 169 DEG C, and air outlet temperature 108 DEG C carries out spray drying and obtains red
Bent pigment yeast powder.Monascorubin purity 34% in monascorubin yeast powder.
Animal experiment proves, Saponin and lovastatin functional food, monascorubin yeast powder and Monascus polysaccharides are functional
Food all has the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.
Schematically being described the present invention and embodiment thereof above, this description does not has restricted, actual knot
Structure is not limited thereto.So, if those of ordinary skill in the art is enlightened by it, without departing from the invention objective
In the case of, design the frame mode similar to this technical scheme and embodiment without creative, all should belong to the present invention's
Protection domain.
Claims (10)
1. one kind utilizes Monascus anka Nakazawa et sato to convert the method that Rhizoma Dioscoreae produces functional food, it is characterised in that described preparation method is with mountain
Medicine and rice, Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers. etc. are solid matrix, with Monascus anka Nakazawa et sato as starting strain, sequentially pass through test tube amplification culture,
Liquid submerged culture and seed tank amplification culture, solid fermentation cultivate, dry, pulverize and prepared by the technique such as packaging；Described merit
Energy property food contains the Saponin of 2～20mg/g butt matter, the Monascus polysaccharides of 10～100mg/g butt matter, 0.5～10mg/g butt
The lovastatin of matter, 1～the monascorubin of 20mg/g butt matter, have regulation blood fat, blood glucose and blood pressure, radioprotective and suppression swollen
The effects such as tumor；Described functional food can extract its active component Saponin, Monascus polysaccharides, lovastatin and monascorubin,
Blood fat, blood glucose and blood pressure, radioprotective and medicine or the functional food such as the tablet of suppression tumor or capsule is reduced for producing treatment
Product.
A kind of Monascus anka Nakazawa et sato the most according to claim 1 convert Rhizoma Dioscoreae produce functional food method, it is characterised in that
The Rhizoma Dioscoreae raw material used includes: RHIIZOMA DIOSCOREAE fr0m Henan of China (Dioscorea oppositaThunb), mesa medicine (Dioscorea alata
L.) and mutation sole sweet potato (Dioscorea alataL.f. flabella Makino.) and include dioscorea japonica
(Dioscorea japonicaThunb.), brown bud potato (Dioscorea persimilisPrain et Burk.), mountain potato
(Dioscorea fordii Prain et Burk.) and spit of fland Rhizoma Dioscoreae (Dioscorea quinquelabaThe Tu Shan such as Thunb.)
Medicine, Rhizoma Dioscoreae (Dioscorea oppositaFoshou) or Fructus Citri Sarcodactylis Rhizoma Dioscoreae (Dioscorea opposita Iron-
rod)；The Monascus anka Nakazawa et sato strain used include monascus (Monascus anka GIM3.592), red monascus (Monascus rubervanTieghem GIM3.240), feathering monascus (Monascus pilosusCGMCC 3.4653), dark brown Monas cuspurpureus Went
Mould (Monascus fuliginosusCGMCC 3.2134), Florida monascus (Monascus floridanus
CGMCC 3.5843), Bark monascus (Monascus barkeriCGMCC 3.4452), monascus aurantiacus (Monascus aurantiacusCGMCC 3.4384), pale monascus (Monascus pallensCGMCC 3.5844) or rust Monas cuspurpureus Went
Mould (Mona -scus rubiginosus CGMCC 3.2844)。
A kind of Monascus anka Nakazawa et sato the most according to claim 1 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that institute
State preparation method to comprise the steps:
A1 test tube amplification culture: Monascus anka Nakazawa et sato slant strains be inoculated in potato dextrose medium and cultivate, prepares red
Aspergillosis test tube slant strain；
A2 liquid submerged culture: the Monascus anka Nakazawa et sato test tube slant strain obtained by step A1 is inoculated into equipped with liquid submerged culture base
Shaking flask in, cultivate, prepare Monascus anka Nakazawa et sato liquid shaking bottle strain；
A3 seed tank amplification culture: be inoculated in seed tank culture base by the Monascus anka Nakazawa et sato liquid shaking bottle strain obtained by step A2
Row is cultivated, and makes Monascus anka Nakazawa et sato seed tank strain；
A4 solid fermentation is cultivated: is inoculated in solid medium by the Monascus anka Nakazawa et sato seed tank strain obtained by step A3, and mixes
Uniformly, carry out fermentation culture, prepare Monascus anka Nakazawa et sato solid fermentation thing；
A5 packs: Monascus anka Nakazawa et sato solid fermentation thing converts the functional food of Rhizoma Dioscoreae through drying, pulverize, pack prepared Monascus anka Nakazawa et sato.
A kind of Monascus anka Nakazawa et sato the most according to claim 3 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that institute
State preparation method specific as follows:
B1 test tube amplification culture: slant strains in Monascus anka Nakazawa et sato test tube is cut into 3 × 3 mm fritter strains, inoculates a fritter to test tube
In slant medium, cultivating 4～15 days for 20～35 DEG C, prepare test tube slant strain, 4 DEG C, this test tube slant saves backup；
B2 liquid submerged culture: the test tube slant strain obtained by step B1 is cut into 3 × 3 mm fritter strains, picking 3～10
Block is inoculated in the 250mL triangular flask equipped with 20～150mL liquid submerged culture bases, triangular flask rotating speed be 50～200 turns/
Point, under conditions of temperature 20～35 DEG C, 18-86h cultivated by shaking table, makes liquid shaking bottle strain；
B3 seed tank amplification culture: the liquid shaking bottle strain obtained by step B2 is inoculated in seed tank amplification culture base, and
Described liquid shaking bottle strain and seed tank amplification culture base are the inoculum concentration inoculation of 1～20% by volume, are 20～35 in temperature
DEG C, speed of agitator is 50～160 revs/min, is 0.2 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio
～under the conditions of the ventilation of 1.8:1, cultivate 18～96h, make Monascus anka Nakazawa et sato seed tank strain；
B4 solid fermentation is cultivated: accessed in solid fermentation culture medium by the Monascus anka Nakazawa et sato seed tank strain obtained by step B3, and institute
State the inoculum concentration inoculation that Monascus anka Nakazawa et sato seed tank strain and solid fermentation culture medium are 2～10% by weight, in temperature 20～35
DEG C, humidity 80% is fermented 5～20 days, and wherein every 3～6 days, stirring once, prepares solid fermentation thing；
B5 solid fermentation thing, after 80～120 DEG C dry 10～24 hours, is crushed to below 100 mesh, is packaged to be and can eat
Functional food.
A kind of Monascus anka Nakazawa et sato the most according to claim 4 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that step
Liquid submerged culture base described in rapid B2 is that sterilizing 10～60 min prepares under the conditions of 100～130 DEG C, and every liter of liquid shaking bottle
Containing wheat bran 5～20g in culture medium, the raw material of the Rhizoma Dioscoreae 5～20g of 10000 revs/min of homogenate, seed tank described in step B3
Amplification culture base is that sterilizing 10～60 min prepares under the conditions of 100～130 DEG C, and containing bran in every liter of liquid submerged culture base
Skin 5～20g, the raw material of the Rhizoma Dioscoreae 5～20g of 10000 revs/min of homogenate, solid fermentation culture medium described in step B4 is 100
～sterilizing 90～360 min prepares under the conditions of 130 DEG C, and the Rhizoma Dioscoreae of 10000 revs/min every kilogram homogenate adds 200～400g
The solid matrix raw materials such as rice, 100～300g Semen Tritici aestivi, 100～300g Semen Maydis and 700～300g Sorghum vulgare Pers..
A kind of Monascus anka Nakazawa et sato the most according to claim 7 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that institute
The ratio stating the 1:0.6～1.5 by weight of the solid matrix in solid fermentation culture medium mixes with water.
A kind of Monascus anka Nakazawa et sato the most according to claim 1 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that step
Extraction Saponin and lovastatin the functional food that Rhizoma Dioscoreae produces is converted from Monascus anka Nakazawa et sato described in rapid A4, A5 or step B4, B5
Method comprise the steps:
Obtained Monascus anka Nakazawa et sato is converted Rhizoma Dioscoreae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium
60～the ethanol of 100% of 5～20 times of volumes of filament weight, repeatedly extracts 3～5 times, obtains extracting solution；
Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times
Water, crystallizes 3～18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains cutting down containing Saponin and Lip river
Statin functional food, this food Saponin and lovastatin content are respectively 25～60% and 30～70%.
A kind of Monascus anka Nakazawa et sato the most according to claim 1 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that step
Convert, from Monascus anka Nakazawa et sato, the method extracting Monascus polysaccharides the functional food that Rhizoma Dioscoreae produces described in rapid A4, A5 or step B4, B5
Comprise the steps:
Obtained Monascus anka Nakazawa et sato mycelium is dried by D1, is then crushed to 20 mesh, adds 5～20 times of volumes of mycelium weight
Distilled water, 80～100 DEG C are repeatedly extracted 3～5 times, obtain extracting solution；
Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, add residual volume 0.5～
95% ethanol of 1.5 times, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extraction raffinate volume 2.5～
95% ethanol of 3.5 times, crystallizes 5～18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains Monascus polysaccharides, Monascus polysaccharides
Purity 20～60%.
A kind of Monascus anka Nakazawa et sato the most according to claim 1 converts the method that Rhizoma Dioscoreae produces functional food, it is characterised in that step
Convert, from Monascus anka Nakazawa et sato, the method extracting monascorubin the functional food that Rhizoma Dioscoreae produces described in rapid A4, A5 or step B4, B5
Comprise the steps:
E1 monascus converts Rhizoma Dioscoreae functional food and pulverizes, and is that 60-100% ethanol is by functional food weight with volume by volume concentration
With the alcohol by volume ratio mixing than 1:10-40,60-90 DEG C is incubated 4-6 hour；Filtering, residue is 60-with volume by volume concentration again
The ratio mixing than 1:10-40 in functional food weight and alcohol by volume of 100% ethanol, 60-90 DEG C is incubated 4-6 hour, repeats
2-3 time；
E2 supernatant macroporous resin static adsorption 5-20 hour of 1/50-1/5 volume, 10-20 times of resin volume after absorption
Water washs, and installs in chromatographic column, and with the 20-80% ethanol desorbing of 10-20 times of resin volume, stripping liquid is incorporated in temperature 20-50
DEG C, it is concentrated in vacuo to the 1/100-1/50 of supernatant volume；
Preferably macroporous resin includes all low pole macroporous resins, such as AB-8 type, D-113 type, D-152 type, HD-2 type, HZD-
3 types, HZD-5 type etc.；
E3 concentrated solution is by regulation flow speed control air inlet temperature 160-190 DEG C, and air outlet temperature 100-120 DEG C carries out spray dried
Dry obtain monascorubin yeast powder；Monascorubin purity 30～60% in monascorubin yeast powder.
10. converting, according to a kind of Monascus anka Nakazawa et sato described in right 1, the method that Rhizoma Dioscoreae produces functional food, step C2, D2 and E3 add
Saponin that work obtains and lovastatin mixture, monascorubin yeast powder and Monascus polysaccharides, can be according to conventional tablet or capsule
Manufacture method is processed into tablet and capsule, and the tablet being processed into and capsule have regulation blood fat, blood glucose and blood pressure, radioprotective and press down
Tumor processed.
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| CN106929428A (en) * | 2017-01-18 | 2017-07-07 | 东莞市双红生物技术有限公司 | The method and product of Monascus polysaccharides are produced in liquid state fermentation |
| CN109259199A (en) * | 2018-10-16 | 2019-01-25 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of coprinus comatus and barley |
| CN109259202A (en) * | 2018-10-16 | 2019-01-25 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn |
| CN109259200A (en) * | 2018-10-16 | 2019-01-25 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed ginger of tremella and corn |
| CN109393444A (en) * | 2018-10-16 | 2019-03-01 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed ginger of coprinus comatus and barley |
| CN110074302A (en) * | 2019-06-04 | 2019-08-02 | 巢湖学院 | A method of drinks are prepared using wheat bran Monascus fermentation broth |
| CN110604277A (en) * | 2019-10-18 | 2019-12-24 | 齐鲁工业大学 | Functional red yeast rice and yam powder as well as preparation method and application thereof |
| CN111394404A (en) * | 2019-05-14 | 2020-07-10 | 江苏臻大天园健康科技有限公司 | Method for producing coenzyme Q10 by using red yeast rice |
| CN115088793A (en) * | 2022-05-20 | 2022-09-23 | 南昌大学 | Red yeast rice and Chinese yam beverage and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929428A (en) * | 2017-01-18 | 2017-07-07 | 东莞市双红生物技术有限公司 | The method and product of Monascus polysaccharides are produced in liquid state fermentation |
| CN109259199A (en) * | 2018-10-16 | 2019-01-25 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of coprinus comatus and barley |
| CN109259202A (en) * | 2018-10-16 | 2019-01-25 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed Chinese yam of tremella and corn |
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| CN109393444A (en) * | 2018-10-16 | 2019-03-01 | 镇江叶脉康食品生物科技有限公司 | The method for preparing blood sugar reducing food using the solid state transformed ginger of coprinus comatus and barley |
| CN111394404A (en) * | 2019-05-14 | 2020-07-10 | 江苏臻大天园健康科技有限公司 | Method for producing coenzyme Q10 by using red yeast rice |
| CN110074302A (en) * | 2019-06-04 | 2019-08-02 | 巢湖学院 | A method of drinks are prepared using wheat bran Monascus fermentation broth |
| CN110604277A (en) * | 2019-10-18 | 2019-12-24 | 齐鲁工业大学 | Functional red yeast rice and yam powder as well as preparation method and application thereof |
| CN115088793A (en) * | 2022-05-20 | 2022-09-23 | 南昌大学 | Red yeast rice and Chinese yam beverage and preparation method thereof |
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Application publication date: 20161123 |