CN106136127A

CN106136127A - A kind of method utilizing Ganoderma to convert Pericarpium Vitis viniferae production functional food

Info

Publication number: CN106136127A
Application number: CN201610543863.2A
Authority: CN
Inventors: 张志才; 李琛
Original assignee: Jiangsu University
Current assignee: Jiangsu University
Priority date: 2016-07-12
Filing date: 2016-07-12
Publication date: 2016-11-23

Abstract

The invention discloses a kind of method utilizing Ganoderma to convert Pericarpium Vitis viniferae production functional food, relate to technical field of bioengineering.Described preparation method sequentially passes through test tube amplification culture, liquid submerged culture and seed tank amplification culture, solid fermentation is cultivated, dry and pulverising step prepares and has regulation blood fat, blood glucose and blood pressure, radioprotective and the functional food of suppression tumor.The present invention, with Pericarpium Vitis viniferae and rice, Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers. etc. as solid matrix, with Ganoderma for strain by solid fermentation, converts Pericarpium Vitis viniferae flavone, simultaneously synthesizing Ganodenic acid and ganoderan；Obtained product, flavones content 2～50mg/g butt matter, ganoderma polyoses content 10～100mg/g butt matter, ganoderic acid content 1～20mg/g butt matter, can be used for extracting its active component flavone, ganoderan and Ganodenic acid, produce treatment and reduce blood fat, blood glucose and blood pressure, radioprotective and the tablet of suppression tumor or capsule.

Description

A kind of method utilizing Ganoderma to convert Pericarpium Vitis viniferae production functional food

Technical field

The present invention relates to technical field of bioengineering, be specifically related to one and utilize Ganoderma to convert the Pericarpium Vitis viniferae functional food of production The method of product.

Background technology

Pericarpium Vitis viniferae is to produce wine and the leftover bits and pieces of Sucus Vitis viniferae, extracts various bioactivators always from Pericarpium Vitis viniferae Being domestic and international study hotspot, these active substances have the strongest non-oxidizability, because having antioxidation in vivo and removing freely The effect of base and the most concerned.The multiple polyphenol product the most abroad obtained from Pericarpium Vitis viniferae, wherein with health product, medicine, change Cosmetic and food are main, and in wine big countries such as France, Italy, Spain, the Pericarpium Vitis viniferae of more than 70% can obtain well Utilize.

China is the most at the early-stage to the research of Fructus Vitis viniferae garbage, and major part is used for feedstuff and fertilizer, and some is then incinerated, The most directly abandoning, utilization rate is extremely low.This is not only the waste of a kind of energy, and environment also can cause certain threat.Along with The development of science and technology, people also begin to gradually pay attention to the development and utilization of Fructus Vitis viniferae garbage, on the one hand can extend Fructus Vitis viniferae and produce Industry chain, turns waste into wealth, and improves income；On the other hand refuse is made to obtain sufficient cycling and reutilization, to the utilization rate improving resource With the development promoting local economy, there is important effect.

Along with the research deepened continuously both at home and abroad finds, grape skin also exists multiple beneficial composition, such as pectin, winestone Acid, Oligomeric Proanthocyanidins, oils and fats, protein etc..Wherein Oligomeric Proanthocyanidins, resveratrol, oleanolic acid, Oleum Vitis viniferae etc. are many Planting functional components, have good medical treatment and health-care effect, therefore grape skin contains huge economic benefit.But pole Rare report utilizes medical edible fungal that Pericarpium Vitis viniferae carries out the research of bioconversion, and the most simple extraction is difficult to make full use of Portugal All functional components of grape skin, therefore the method for all functional components that exploitation makes full use of Pericarpium Vitis viniferae seems particularly necessary.

Inventor, through further investigation and industrialized production test repeatedly, finds out industrially scalable and converts Pericarpium Vitis viniferae Producing functional food technology, no matter the present invention from the mode of production and the product of production of product, is different from conventional patent With the content of article report, a kind of method utilizing Ganoderma to convert Pericarpium Vitis viniferae production functional food.

Summary of the invention

, problem to be solved

For the above-mentioned problems in the prior art, the present invention provides one to utilize Ganoderma to convert Pericarpium Vitis viniferae and produces functional food The method of product, it is with Pericarpium Vitis viniferae and rice, Semen Tritici aestivi, Semen Maydis and Sorghum vulgare Pers. as solid matrix, with Ganoderma for strain by solid fermentation, Convert Pericarpium Vitis viniferae, make Ganoderma mycelium have the functional food of pharmacy function with Pericarpium Vitis viniferae interaction production by fermentation Product, technique is simple, and in obtained product, flavones content 2～50mg/g butt matter, ganoderma polyoses content 10～100mg/g Butt matter, ganoderic acid content 1～20mg/g butt matter.This product thus can also extract Ganodenic acid and flavone, polysaccharide further The product prepared has the pharmacognosy function identical with fermented product.

, technical scheme

In order to solve the problems referred to above, the technical solution adopted in the present invention is as follows:

Described a kind of utilizing Ganoderma to convert the method that Pericarpium Vitis viniferae produces functional food, described preparation method is with Pericarpium Vitis viniferae, big Rice, Semen Tritici aestivi, Semen Maydis and Sorghum vulgare Pers. are main matrix, with Ganoderma as starting strain, sequentially pass through test tube amplification culture, liquid shaking bottle training Support and the step such as seed tank amplification culture, solid fermentation cultivation；Described functional food can prepare Huang respectively through extracting again Ketone and Ganodenic acid extract, ganoderan extract, wherein in flavone and Ganodenic acid extract, flavone and ganoderic acid content be respectively It is 25～60% and 30～70%, ganoderan purity 20～60%.

Described a kind of Ganoderma convert Pericarpium Vitis viniferae produce functional food method, the Pericarpium Vitis viniferae that the method is used is former Material is Pericarpium Vitis viniferae or the Pericarpium Vitis viniferae of Sucus Vitis viniferae factory production of Production of Wine factory；The Ganderma lucidum strain used includes Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum), Ganoderma (Ganoderma lucidum) or Ganoderma (Ganoderma sinensis)。

A kind of Ganoderma converts the method that Pericarpium Vitis viniferae produces functional food, carries out as steps described below:

A1 test tube amplification culture: Ganoderma slant strains be inoculated in potato dextrose medium and cultivate, prepares Ganoderma Test tube slant strain；

A2 liquid submerged culture: the Ganoderma test tube slant strain obtained by step A1 is inoculated into equipped with liquid submerged culture base In shaking flask, cultivate, prepare ganoderma lucidum liquid shaking flask strain；

A3 seed tank amplification culture: the ganoderma lucidum liquid shaking flask strain obtained by step A2 is inoculated in seed tank culture base and carries out Cultivate, make Ganoderma seed tank strain；

A4 solid fermentation is cultivated: be inoculated in solid medium by the Ganoderma seed tank strain obtained by step A3, and mixes all Even, carry out fermentation culture, prepare Ganoderma solid fermentation thing；

A5 packs: Ganoderma solid fermentation thing converts the functional food of Pericarpium Vitis viniferae through drying, pulverize, pack prepared Ganoderma.

Preferably, a kind of Ganoderma convert Pericarpium Vitis viniferae produce functional food method, carry out as steps described below:

B1 test tube amplification culture: slant strains in Ganoderma test tube is cut into 3 × 3 mm fritter strains, inoculates a fritter oblique to test tube In the culture medium of face, cultivating 4～15 days for 20～35 DEG C, prepare test tube slant strain, 4 DEG C, this test tube slant saves backup；

B2 liquid submerged culture: the test tube slant strain obtained by step B1 is cut into 3 × 3 mm fritter strains, picking 3～10 Block is inoculated in the 250mL triangular flask equipped with 20～150mL liquid submerged culture bases, triangular flask rotating speed be 50～200 turns/ Point, under conditions of temperature 20～35 DEG C, cultivate 18-86h, make liquid shaking bottle strain；

B3 seed tank amplification culture: the liquid shaking bottle strain obtained by step B2 is inoculated in seed tank amplification culture base, and Described liquid shaking bottle strain and seed tank amplification culture base are the inoculum concentration inoculation of 1～20% by volume, are 20～35 in temperature DEG C, speed of agitator is 50～160 revs/min, is 0.2 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio ～under the conditions of the ventilation of 1.8:1, cultivate 18～96h, make Ganoderma seed tank strain；

B4 solid fermentation is cultivated: accessed in solid fermentation culture medium by the Ganoderma seed tank strain obtained by step B3, and described Ganoderma seed tank strain and solid fermentation culture medium are the inoculum concentration inoculation of 2～10% by weight, temperature 20～35 DEG C, wet Degree 80% fermentation 5～20 days, wherein every 3～6 days, stirring once, prepares solid fermentation thing；

B5 solid fermentation thing, after 80～120 DEG C dry 10～24 hours, is crushed to below 100 mesh, is packaged to be and can eat Functional food；

Step A4, A5 or step B4, B5 functional food carry out the extracting method of Ganodenic acid and flavone and comprise the steps:

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium 60～the ethanol of 100% of 5～20 times of volumes of filament weight, repeatedly extracts 3～5 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 3～18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganoderma Acid function food, this food flavone and ganoderic acid content are respectively 25～60% and 30～70%.

Step A4, A5 or step B4, B5 functional food carry out the extraction of ganoderan and comprise the steps:

Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the steaming of 5～20 times of volumes of mycelium weight Distilled water, 80～100 DEG C are repeatedly extracted 3～5 times, obtain extracting solution；

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, add residual volume 0.5～ 95% ethanol of 1.5 times, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extraction raffinate volume 2.5～ 95% ethanol of 3.5 times, crystallizes 5～18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan Purity 20～60%.

Preferably, liquid submerged culture base described in step B2 is sterilizing 10～60 min system under the conditions of 100～130 DEG C , and containing wheat bran 5～20g, the raw material of Pericarpium Vitis viniferae 5～20g in every liter of liquid submerged culture base.

Preferably, the base of seed tank amplification culture described in step B3 is sterilizing 10～60 min system under the conditions of 100～130 DEG C , and containing wheat bran 5～20g, the raw material of Pericarpium Vitis viniferae 5～20g in every liter of liquid submerged culture base.

Preferably, solid fermentation culture medium described in step B4 is sterilizing 90～360 min under the conditions of 100～130 DEG C Prepare, and every 500 grams of Pericarpium Vitis viniferaes add 200～400g rice, 100～300g Semen Tritici aestivi, 100～300g Semen Maydis and 700～ The solid matrix raw materials such as 300g Sorghum vulgare Pers..

Preferably, the raw material in described solid fermentation culture medium mixes with water by weight the ratio for 1:0.6～1.5.

, beneficial effect

Compared to prior art, the invention have the benefit that

(1) present invention is on the basis of drawing herb fermenting pharmaceutical technology, in conjunction with the advantage of modern solid-state fermentation, uses solid-state to send out Ferment converts Pericarpium Vitis viniferae, and during overcoming liquid fermentation, the suppression of Mycelium Growth of Ganoderma lucidum is made by substrate (active component of Pericarpium Vitis viniferae) With, simultaneously without liquid fermentation waste water and residue contamination；

(2) a kind of method utilizing Ganoderma to convert Pericarpium Vitis viniferae production functional food of the present invention, is not simple utilization Then the active component of active component and Ganoderma that process for separation and purification extracts Pericarpium Vitis viniferae carries out compounding forming, but with Ganoderma is Strain passes through solid fermentation, directly eats, both improve effect of Pericarpium Vitis viniferae active component, enhanced again spirit after converting Pericarpium Vitis viniferae Sesame active component ganoderan and the amount of Ganodenic acid, can realize industrialization large-scale production；

(3) a kind of Ganoderma of the present invention convert Pericarpium Vitis viniferae produce functional food method, in its product flavones content 2～ 50mg/g butt matter, ganoderma polyoses content 10～100mg/g butt matter, ganoderic acid content 1～20mg/g butt matter.There is regulation The effects such as blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.The Ganodenic acid extracted from this product and flavone, ganoderan There is regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor function equally.

Accompanying drawing explanation

Fig. 1 is the preparation flow of a kind of method utilizing Ganoderma to convert Pericarpium Vitis viniferae production functional food of the present invention Schematic diagram.

Detailed description of the invention

The mensuration of described flavones content employing sodium nitrite-aluminum nitrate assay method (Deng Bin, Jiang Gangbiao, old Six is flat. the content [J] of Food wrapping paper Rhizoma Dioscoreae esculentae total flavones. and Packaging Engineering, 2008,29 (1): 27 ~ 29).

The mensuration of described ganoderic acid content uses ultraviolet spectrophotometry, and (Wang Xiangfei, Pan Xudong, Liu Wenxia, etc. Hemerocallis citrina Baroni Herba Orostachyos The mensuration of the content of middle total triterpene composition. China National folk medicine, 2009,18 (10): 11).

Described ganoderma polyoses content deducts reducing sugar equal to total sugar, and the mensuration of total sugar uses sulfuric acid-phynol method (Zhaoyang Nanmu, Chang Jidong. Phenol sulfuric acid procedure and indirect iodometric processes measure ganoderma polyoses content and compare [J]. edible fungi, 2007, (3): 58 ～61.), content of reducing sugar measures employing 3,5-dinitrosalicylic acid system (Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Chemistry. 1959,1:426 ~ 428.).

Described Ganoderma converts Pericarpium Vitis viniferae and produces the evaluation methodology employing high blood lipid model Mus of functional food regulation blood fat Evaluate (Cao Min etc.: sanchi flower total saponine hypotensive effect is studied. the bright traditional Chinese medical science, and 2012,27 (7): 1314-1315).Concrete operations For: after 40 mices adapt to feed 5d with normal feedstuff, take tail hematometry Triglycerides in Serum content, with triglyceride Level is randomly divided into 4 groups: Normal group, hyperlipidemia matched group, low dosage experimental group, high dose experimental group.Normal control Group mice feed normal feedstuff (12% casein, 60.98% corn starch, 15% sucrose, 7% Semen Maydis oil, 1% vitamin Icing Sugar, 4% Mineral salt, 0.02% cod-liver oil)；(2% gallbladder is solid for 15.75% lard stearin, 7.79% sucrose for hyperlipidemia model control group fed high lipid food Alcohol, 0.5% cholic acid, 73.96% normal feedstuff；Low dosage and high dose group mice feed per kilogram high lipid food and contain 10 grams and 20 Gram Ganoderma convert Pericarpium Vitis viniferae functional food.Each group mice the most freely ingests and drinks water, animal housing's temperature 18～22 DEG C.Continuously After being administered surrounding, tail vein takes blood, analyzes serum triglyceride, T-CHOL, high density lipoprotein and low density lipoprotein Protein content

Described Ganoderma converts Pericarpium Vitis viniferae and produces the evaluation methodology employing barbital of functional food regulation blood fat and ligature left kidney (yellow will is new: Herba Visci, the hypotensive activity of the Cortex Eucommiae and acute toxicity in the Hypertensive Rats evaluation of tremulous pulse combined induction Experimentation. research and development of natural products, 2003,15 (3): 245-248).Hypertension model Mus is divided into three groups of blanks The Ganoderma that group, low dose group and high dose group feed normal feedstuff respectively, per kilogram contains 10 grams converts Rhizoma Dioscoreae functional food Normal feedstuff and per kilogram contain 20 grams Ganoderma convert Rhizoma Dioscoreae functional food normal feedstuff.Successive administration measures for 14 days Blood pressure before being administered and after administration.

Described Ganoderma converts Pericarpium Vitis viniferae and produces the evaluation methodology employing alloxan induction of functional food regulation blood glucose Diabetic rat model evaluation.Induction reference literature (the Effect of FeSO such as Zhicai Zhang of model mouse₄ treatment on glucose metabolism in diabetic rats. Biometals (2008) 21:685– 691) carry out.Hyperglycemia model Mus is divided into three groups of blank groups, low dose group and high dose group to feed normal feedstuff, every respectively Kilogram convert the normal feedstuff of Rhizoma Dioscoreae functional food containing the Ganoderma of 10 grams and Ganoderma that per kilogram contains 20 grams converts Rhizoma Dioscoreae The normal feedstuff of functional food.Successive administration measures the blood glucose value before being administered and after administration for 28 days.

Described Ganoderma converts the Pericarpium Vitis viniferae production radiation-resistant evaluation methodology of functional food and uses radiation irradiation in rats to enter Row evaluation (a diplodocus man of virtue and ability. the effect study of Lac regis apis Antiradiation injury. Heilungkiang medical science, 2012,36 (10): 724-726).30 SD male rat feeds normal feedstuff respectively, converts the normal feedstuff 15 days of Pericarpium Vitis viniferae functional food containing 1% and 2% Ganoderma After, rat is feeding⁶⁰Co gamma-rays carries out total irradiation, away from radiation source 1.0 m close rate 2.0 Gy, accumulated dose 200rad.Irradiate Measuring body weight, mouse mortality rate after 10 days, tail vein takes haemanalysis quantity of leucocyte.

Described Ganoderma converts Pericarpium Vitis viniferae and produces the evaluation methodology employing suppression Primary Hepatic of functional food suppression tumor Cancerous cell Hepg2 growth effect (Zhouning County, Chen Jiangtao, Yu Wenyan. Water Extract of Ficus Carica to suppression tumor cell proliferation The preliminary study of effect. Xinjiang Medicine University's journal. 2016,39 (1): 42-47).Hepg2 groups of cells complete culture solution Adjusting cell concentration is 2 × 10⁴Individual/mL, is inoculated in 96 orifice plates, and every hole cumulative volume is 200 μ L, and overnight incubation makes cell attachment, Next day respectively with the Ganoderma of various dose convert the ethanol extract of Pericarpium Vitis viniferae functional food and aqueous extract (substrate: water (or Dehydrated alcohol)=1:10,70 DEG C are extracted 4 hours) and processing cell 24h respectively, matched group adds PBS and processes 24h, and each mensuration connects Plant 3 multiple holes, be placed in 37 DEG C, 5%CO₂After incubator processes 24, every hole adds 5mg/mL tetrazolium bromide (MTT) reagent 20 μ L, continues training Support 4h, then add dimethyl sulfoxide (DMSO) 150 μ L, vibration mixing 10min, with microplate reader (λ=490nm) measure OD value (OD value and Viable count is directly proportional), cell inhibitory rate=1-(dosing group OD value/matched group OD value), experiment is repeated 3 times.

Below in conjunction with specific embodiment, the present invention is further described below.

Embodiment 1

Utilize Ganoderma to convert Pericarpium Vitis viniferae to produce the method for functional food and specifically include following steps as it is shown in figure 1, a kind of:

(1) test tube amplification culture: Ganoderma (Ganoderma sinense) CGMCC 5.69 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 15 days for 20 DEG C, prepare test tube slant strain, this test tube 4 DEG C of inclined-plane saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 10 pieces Being inoculated in the 250mL triangular flask equipped with 150mL liquid submerged culture base, triangular flask is 50 revs/min at rotating speed, temperature 35 DEG C Under the conditions of, 18h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 10 under the conditions of 130 DEG C Min prepares, and containing wheat bran 5g, the raw material of Pericarpium Vitis viniferae 20g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 1% by volume, it is 35 DEG C in temperature, stirs Mixing rotating speed is 160 revs/min, is the ventilation of 0.2:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of amount, cultivate 96h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 10 under the conditions of 130 DEG C Min prepares, and containing wheat bran 5g, the raw material of Pericarpium Vitis viniferae 20g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: the Ganoderma seed tank strain obtained by step (3) is accessed the Fructus Vitis viniferae equipped with Production of Wine factory In skin solid fermentation culture medium, and described Ganoderma seed tank strain and solid fermentation culture medium are the inoculum concentration of 10% by weight Inoculation, temperature 20 DEG C, humidity 80% is fermented 40 days, and wherein every 6 days, stirring once, prepares solid fermentation thing；Described solid Fermentation medium is that sterilizing 360 min prepares under the conditions of 130 DEG C, and solid medium adds 200g by every 500 grams of Pericarpium Vitis viniferaes The solid matrix raw materials such as rice, 100g Semen Tritici aestivi, 100g Semen Maydis and 700g Sorghum vulgare Pers. prepare；Raw material in described solid fermentation culture medium Mix with water by weight the ratio for 1:1.1；

(5) by the solid fermentation thing obtained by step (4) after 120 DEG C dry 10 hours, it is crushed to below 100 mesh, packaging Obtain edible functional food；Described functional food contains the flavone of 2mg/g butt matter, 10mg/g butt matter Ganoderan, the Ganodenic acid of 1mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-spoke Penetrate and suppress the effects such as tumor；

(6) extract separate: by obtained by step (4) or (5) Ganoderma convert Pericarpium Vitis viniferae produce functional food respectively through Extract, respectively obtain containing flavone and the product of Ganodenic acid and the product of ganoderan.

It should be noted that and comprise the steps: from the method for the product containing flavone and Ganodenic acid described in step (6)

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 60% of 5 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 3 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid merit Energy property food, this food flavone and ganoderic acid content are respectively 25% and 70%.

In the present embodiment, comprise the steps: from the method containing ganoderan product described in step (6)

Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 5 times of volumes of mycelium weight Water, 80 DEG C are repeatedly extracted 5 times, obtain extracting solution；

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.5 times 95% ethanol, under the conditions of 4 DEG C crystallize 5 hours, filter, filtrate continuously add extract raffinate volume 2.5 times 95% ethanol, Crystallizing 5 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 20%；

Animal experiment proves, flavone and Ganodenic acid functional food and ganoderan functional food all have regulation blood fat, blood The effects such as sugar and blood pressure, radioprotective and suppression tumor.

Embodiment 2

A kind of Ganoderma convert Pericarpium Vitis viniferae produce functional food method specifically include following steps:

(1) test tube amplification culture: by Ganoderma lucidum (Leyss. Ex Fr.) Karst. (Ganoderma lucidum) CICC 14045 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 4 days for 35 DEG C, prepare test tube slant strain, and this test tube is oblique 4 DEG C of face saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 3 pieces Being inoculated in the 250mL triangular flask equipped with 20mL liquid submerged culture base, triangular flask is 200 revs/min at rotating speed, temperature 20 DEG C Under the conditions of, 86h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 60 under the conditions of 100 DEG C Min prepares, and containing bran 20g, the raw material of Pericarpium Vitis viniferae 5g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 20% by volume, it is 20 DEG C in temperature, stirs Mixing rotating speed is 50 revs/min, is 0.2～1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of ventilation, cultivate 18h, make Ganoderma seed tank strain；Described seed tank amplification culture base is to go out under the conditions of 100 DEG C Bacterium 60min prepares, and containing wheat bran 20g, the raw material of Pericarpium Vitis viniferae 5g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: access the Ganoderma seed tank strain obtained by step (3) equipped with the production of Sucus Vitis viniferae factory In Pericarpium Vitis viniferae solid fermentation culture medium, and described Ganoderma seed tank strain and solid fermentation culture medium are the inoculation of 2% by weight Amount inoculation, temperature 35 DEG C, humidity 80% is fermented 20 days, and wherein every 3 days, stirring once, prepares solid fermentation thing；Described solid Body fermentation medium is that sterilizing 90min prepares under the conditions of 100 DEG C, and solid medium adds 400g by every 500 grams of Pericarpium Vitis viniferaes The solid matrix raw materials such as rice, 300g Semen Tritici aestivi, 300g Semen Maydis and 300g Sorghum vulgare Pers. prepare；Raw material in described solid fermentation culture medium Mix with water by weight the ratio for 1:1.5；

(5) by the solid fermentation thing obtained by step (4) after 80 DEG C dry 10 hours, it is crushed to below 100 mesh, packs To edible functional food；Described functional food contains the flavone of 50mg/g butt matter, 100mg/g butt matter Ganoderan, the Ganodenic acid of 20mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti- The effects such as radiation and suppression tumor；

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 100% of 20 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 18 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid Functional food, this food flavone and ganoderic acid content are respectively 60% and 30%.

Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 20 times of volumes of mycelium weight Water, 100 DEG C are repeatedly extracted 3 times, obtain extracting solution；

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.5 times 95% ethanol, under the conditions of 4 DEG C crystallize 5～18 hours, filter, filtrate continuously add extract raffinate volume 3.5 times 95% Ethanol, crystallizes 18 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 60%；

Embodiment 3

(1) test tube amplification culture: by Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum) GIM5.282 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 10 days for 28 DEG C, prepare test tube slant strain, this test tube 4 DEG C of inclined-plane saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 7 pieces Being inoculated in the 250mL triangular flask equipped with 80mL liquid submerged culture base, triangular flask is 120 revs/min at rotating speed, temperature 28 DEG C Under the conditions of, 57h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 35 under the conditions of 120 DEG C Min prepares, and containing wheat bran 13g, the raw material of Pericarpium Vitis viniferae 13g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 10% by volume, it is 28 DEG C in temperature, stirs Mixing rotating speed is 110 revs/min, is the ventilation of 1:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of, cultivate 57h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing under the conditions of 120 DEG C 35min prepares, and containing wheat bran 13g, the raw material of Pericarpium Vitis viniferae 13g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: the Ganoderma seed tank strain obtained by step (3) is accessed the Fructus Vitis viniferae equipped with Production of Wine factory In skin solid fermentation culture medium, and described Ganoderma seed tank strain with solid fermentation culture medium be by weight 6% inoculum concentration connect Kind, temperature 28 DEG C, humidity 80% is fermented 30 days, and wherein every 4.5 days, stirring once, prepares solid fermentation thing；Described solid Fermentation medium is that sterilizing 260 min prepares under the conditions of 120 DEG C, and solid medium adds 300g by every 500 grams of Pericarpium Vitis viniferaes The solid matrix raw materials such as rice, 200g Semen Tritici aestivi, 200g Semen Maydis and 500g Sorghum vulgare Pers. prepare；Raw material in described solid fermentation culture medium Ratio by weight 1:0.6 mixes with water；

(5) solid fermentation thing is after 100 DEG C dry 17 hours, is crushed to below 100 mesh, is packaged to be edible function Property food；Described functional food contains the flavone of 27.5mg/g butt matter, the ganoderan of 54mg/g butt matter, 11mg/g The Ganodenic acid of butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc. Effect；

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 80% of 12 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 11 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid Functional food, this food flavone and ganoderic acid content are respectively 43% and 50%.

Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 13 times of volumes of mycelium weight Water, 90 DEG C are repeatedly extracted 4 times, obtain extracting solution；

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times 95% ethanol, crystallizes 12 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3 times, in 4 Crystallizing 12 hours under the conditions of DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 41%；

Embodiment 4

(1) test tube amplification culture: Ganoderma (Ganoderma sinense) CGMCC 5.69 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 12 days for 23 DEG C, prepare test tube slant strain, this test tube 4 DEG C of inclined-plane saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 8 pieces Being inoculated in the 250mL triangular flask equipped with 30mL liquid submerged culture base, triangular flask is 75 revs/min at rotating speed, temperature 20～35 Under conditions of DEG C, 30h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing under the conditions of 100 DEG C 55 min prepare, and containing wheat bran 8g, the raw material of Pericarpium Vitis viniferae 12g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 4% by volume, it is 25 DEG C in temperature, stirs Mixing rotating speed is 120 revs/min, is the ventilation of 0.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of amount, cultivate 35h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 55 under the conditions of 100 DEG C Min prepares, and containing wheat bran 8g, the raw material of Pericarpium Vitis viniferae 15g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: accesses the Ganoderma seed tank strain obtained by step (3) to produce equipped with Sucus Vitis viniferae and produces factory's life In Pericarpium Vitis viniferae solid fermentation culture medium, and described Ganoderma seed tank strain and solid fermentation culture medium are the inoculation of 4% by weight Amount inoculation, temperature 22 DEG C, humidity 80% is fermented 25 days, and wherein every 3 days, stirring once, prepares solid fermentation thing；Described solid Body fermentation medium is that sterilizing 100 min prepares under the conditions of 100 DEG C, and solid medium is added by every 500 grams of Pericarpium Vitis viniferaes The solid matrix raw materials such as 250g rice, 150g Semen Tritici aestivi, 150g Semen Maydis and 600g Sorghum vulgare Pers. prepare；In described solid fermentation culture medium Raw material mixes with water by weight the ratio of 1:1.0；

(5) by the solid fermentation thing obtained by step (4) after 90 DEG C dry 22 hours, it is crushed to below 100 mesh, packs To edible functional food；Described functional food contains the flavone of 45mg/g butt matter, 90mg/g butt matter Ganoderan, the Ganodenic acid of 5mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti-spoke Penetrate and suppress the effects such as tumor；

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 70% of 7 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 6 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid merit Energy property food, this food flavone and ganoderic acid content are respectively 31% and 41%.

Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 7 times of volumes of mycelium weight Water, 85 DEG C are repeatedly extracted 5 times, obtain extracting solution；

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.7 times 95% ethanol, under the conditions of 4 DEG C crystallize 7 hours, filter, filtrate continuously add extract raffinate volume 2.8 times 95% ethanol, Crystallizing 7 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 31%；

Embodiment 5

(1) test tube amplification culture: by Ganoderma lucidum (Leyss. Ex Fr.) Karst. (Ganoderma lucidum) CICC 14045 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 6 days for 32 DEG C, prepare test tube slant strain, and this test tube is oblique 4 DEG C of face saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces Being inoculated in the 250mL triangular flask equipped with 40mL liquid submerged culture base, triangular flask is 160 revs/min at rotating speed, temperature 30 DEG C Under the conditions of, 36h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 28 under the conditions of 128 DEG C Min prepares, and containing wheat bran 6g, the raw material of Pericarpium Vitis viniferae 20g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 30 DEG C in temperature, stirs Mixing rotating speed is 160 revs/min, is the ventilation of 0.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of amount, cultivate 68h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 28 under the conditions of 128 DEG C Min prepares, and containing wheat bran 15g, the raw material of Pericarpium Vitis viniferae 7g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: the Ganoderma seed tank strain obtained by step (3) accesses the Pericarpium Vitis viniferae equipped with grape wine factory solid In body fermentation medium, and described Ganoderma seed tank strain is inoculated with the inoculum concentration that solid fermentation culture medium is 6% by weight, Temperature 30 DEG C, humidity 80% is fermented 28 days, and wherein every 5 days, stirring once, prepares solid fermentation thing；Described solid fermentation is trained Foster base is that sterilizing 320 min prepares under the conditions of 128 DEG C, and solid medium by every 500 grams of Pericarpium Vitis viniferaes add 350g rice, The solid matrix raw materials such as 250g Semen Tritici aestivi, 250g Semen Maydis and 400g Sorghum vulgare Pers. prepare；Raw material in described solid fermentation culture medium is by weight The amount ratio than 1:0.6 mixes with water；

(5) solid fermentation thing is after 90 DEG C dry 18 hours, is crushed to below 100 mesh, is packaged to be edible function Property food；Described functional food contains the flavone of 10mg/g butt matter, and the ganoderan of 24mg/g butt matter, 16mg/g are dry The Ganodenic acid of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor Effect；

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 90% of 17 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 15 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid Functional food, this food flavone and ganoderic acid content are respectively 25% and 61%.

Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 18 times of volumes of mycelium weight Water, 95 DEG C are repeatedly extracted 3 times, obtain extracting solution；

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.4 times 95% ethanol, under the conditions of 4 DEG C crystallize 16 hours, filter, filtrate continuously add extract raffinate volume 3.2 times 95% second Alcohol, crystallizes 16 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 52%；

Embodiment 6

(1) test tube amplification culture: by Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum) GIM5.282 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 5 days for 32 DEG C, prepare test tube slant strain, and this test tube is oblique 4 DEG C of face saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces Being inoculated in the 250mL triangular flask equipped with 20mL liquid submerged culture base, triangular flask is 170 revs/min at rotating speed, temperature 25 DEG C Under the conditions of, 60h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 50 under the conditions of 110 DEG C Min prepares, and containing wheat bran 16g, the raw material of Pericarpium Vitis viniferae 8g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 25 DEG C in temperature, stirs Mixing rotating speed is 120 revs/min, is the ventilation of 1.6:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of amount, cultivate 29h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 50 under the conditions of 110 DEG C Min prepares, and containing wheat bran 14g, the raw material of Pericarpium Vitis viniferae 10g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: the Ganoderma seed tank strain obtained by step (3) accesses the Pericarpium Vitis viniferae equipped with grape wine factory solid In body fermentation medium, and described Ganoderma seed tank strain is inoculated with the inoculum concentration that solid fermentation culture medium is 6% by weight, Temperature 25 DEG C, humidity 80% is fermented 26 days, and wherein every 5 days, stirring once, prepares solid fermentation thing；Described solid fermentation is trained Foster base is that sterilizing 250 min prepares under the conditions of 110 DEG C, and solid medium by every 500 grams of Pericarpium Vitis viniferaes add 400g rice, The solid matrix raw materials such as 150g Semen Tritici aestivi, 150g Semen Maydis and 500g Sorghum vulgare Pers. prepare；Raw material in described solid fermentation culture medium is by weight The amount ratio than 1:0.8 mixes with water；

(5) by the solid fermentation thing obtained by step (4) after 110 DEG C dry 16 hours, it is crushed to below 100 mesh, packaging Obtain edible functional food；Described functional food contains the flavone of 15mg/g butt matter, 56mg/g butt matter Ganoderan, the Ganodenic acid of 18mg/g butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, anti- The effects such as radiation and suppression tumor；

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 63% of 11 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 12 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid Functional food, this food flavone and ganoderic acid content are respectively 55% and 37%.

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times 95% ethanol, crystallizes 13 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3.0 times, Crystallizing 13 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 45%；

Embodiment 7

(1) test tube amplification culture: Ganoderma (Ganoderma sinense) CGMCC 5.69 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 13 days for 23 DEG C, prepare test tube slant strain, this test tube 4 DEG C of inclined-plane saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 10 pieces Being inoculated in the 250mL triangular flask equipped with 130mL liquid submerged culture base, triangular flask is 150 revs/min at rotating speed, temperature 23 DEG C Under conditions of, 36h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 53 under the conditions of 115 DEG C Min prepares, and containing wheat bran 9g, the raw material of Pericarpium Vitis viniferae 20g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 15% by volume, it is 21 DEG C in temperature, stirs Mixing rotating speed is 120 revs/min, is the ventilation of 0.2:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of amount, cultivate 81h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 53 under the conditions of 115 DEG C Min prepares, and containing wheat bran 19g, the raw material of Pericarpium Vitis viniferae 18g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: the Ganoderma seed tank strain obtained by step (3) accesses the Pericarpium Vitis viniferae equipped with Sucus Vitis viniferae factory solid In body fermentation medium, and described Ganoderma seed tank strain is inoculated with the inoculum concentration that solid fermentation culture medium is 4% by weight, Temperature 21 DEG C, humidity 80% is fermented 34 days, and wherein every 6 days, stirring once, prepares solid fermentation thing；Described solid fermentation is trained Foster base is that sterilizing 250 min prepares under the conditions of 110 DEG C, and solid medium by every 500 grams of Pericarpium Vitis viniferaes add 400g rice, The solid matrix raw materials such as 150g Semen Tritici aestivi, 250g Semen Maydis and 400g Sorghum vulgare Pers. prepare；Raw material in described solid fermentation culture medium is by weight The amount ratio than 1:1.0 mixes with water；

(5) solid fermentation thing is after 115 DEG C dry 16 hours, is crushed to below 100 mesh, is packaged to be edible function Property food；Described functional food contains the flavone of 50mg/g butt matter, the ganoderan of 100mg/g butt matter, 1mg/g The Ganodenic acid of butt matter, animal experiment proves, this product has regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor etc. Effect；

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 75% of 9 times of volumes of filament weight, repeatedly extracts 5 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 10 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid Functional food, this food flavone and ganoderic acid content are respectively 38% and 45%.

Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 10 times of volumes of mycelium weight Water, 88 DEG C are repeatedly extracted 5 times, obtain extracting solution；

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 0.8 times 95% ethanol, under the conditions of 4 DEG C crystallize 10 hours, filter, filtrate continuously add extract raffinate volume 2.9 times 95% second Alcohol, crystallizes 10 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 38%；

Embodiment 8

(1) test tube amplification culture: by Ganoderma lucidum (Leyss. Ex Fr.) Karst. (Ganoderma lucidum) CICC 14045 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 10 days for 29 DEG C, prepare test tube slant strain, this test tube 4 DEG C of inclined-plane saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 8 pieces Being inoculated in the 250mL triangular flask equipped with 60mL liquid submerged culture base, triangular flask is 160 revs/min at rotating speed, temperature 33 DEG C Under the conditions of, 80h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 30 under the conditions of 120 DEG C Min prepares, and containing wheat bran 19g, the raw material of Pericarpium Vitis viniferae 19g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 18% by volume, it is 33 DEG C in temperature, stirs Mixing rotating speed is 140 revs/min, is the ventilation of 1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of amount, cultivate 90h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 30 under the conditions of 120 DEG C Min prepares, and containing wheat bran 18g, the raw material of Pericarpium Vitis viniferae 18g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: the Ganoderma seed tank strain obtained by step (3) accesses the Pericarpium Vitis viniferae equipped with grape wine factory solid In body fermentation medium, and described Ganoderma seed tank strain is inoculated with the inoculum concentration that solid fermentation culture medium is 9% by weight, Temperature 33 DEG C, humidity 80% is fermented 37 days, and wherein every 6 days, stirring once, prepares solid fermentation thing；Described solid fermentation is trained Foster base is that sterilizing 350 min prepares under the conditions of 105 DEG C, and solid medium by every 500 grams of Pericarpium Vitis viniferaes add 280g rice, The solid matrix raw materials such as 220g Semen Tritici aestivi, 270g Semen Maydis and 380g Sorghum vulgare Pers. prepare；Raw material in described solid fermentation culture medium is by weight The amount ratio than 1:1.4 mixes with water；

(5) solid fermentation thing is after 85 DEG C dry 23 hours, is crushed to below 100 mesh, is packaged to be edible function Property food；Described functional food contains the flavone of 45mg/g butt matter, and the ganoderan of 94mg/g butt matter, 3mg/g are dry The Ganodenic acid of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor Effect；

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 97% of 16 times of volumes of filament weight, repeatedly extracts 3 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 15 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid Functional food, this food flavone and ganoderic acid content are respectively 26% and 67%.

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1.4 times 95% ethanol, under the conditions of 4 DEG C crystallize 16 hours, filter, filtrate continuously add extract raffinate volume 3.2 times 95% second Alcohol, crystallizes 6 hours under the conditions of 4 DEG C, and then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 57%；

Embodiment 9

(1) test tube amplification culture: by Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum) GIM5.282 test tube slant strain is cut into 3 × 3 Mm fritter strain, inoculates a fritter in test tube slant culture medium, cultivates 6 days for 24 DEG C, prepare test tube slant strain, and this test tube is oblique 4 DEG C of face saves backup；

(2) liquid submerged culture: the test tube slant strain obtained by step (1) is cut into 3 × 3 mm fritter strains, picking 4 pieces Being inoculated in the 250mL triangular flask equipped with 24mL liquid submerged culture base, triangular flask is 58 revs/min at rotating speed, temperature 24 DEG C Under the conditions of, 20h cultivated by shaking table, makes liquid shaking bottle strain；Described liquid submerged culture base is sterilizing 59 under the conditions of 100 DEG C Min prepares, and containing wheat bran 5g, the raw material of Pericarpium Vitis viniferae 5g in every liter of liquid submerged culture base；

(3) seed tank amplification culture: the liquid shaking bottle strain obtained by step (2) is inoculated in seed tank amplification culture base, And described liquid shaking bottle strain and the inoculum concentration inoculation that seed tank amplification culture base is 3% by volume, it is 24 DEG C in temperature, stirs Mixing rotating speed is 58 revs/min, is the ventilation of 1.8:1 at the volume being passed through gas per minute and seed tank amplification culture base volume ratio Under the conditions of, cultivate 25h, make Ganoderma seed tank strain；Described seed tank amplification culture base is sterilizing 59 under the conditions of 100 DEG C Min prepares, and containing wheat bran 5g, the raw material of Pericarpium Vitis viniferae 6g in every liter of liquid submerged culture base；

(4) solid fermentation is cultivated: the Ganoderma seed tank strain obtained by step (3) accesses the Pericarpium Vitis viniferae equipped with grape wine factory solid In body fermentation medium, and described Ganoderma seed tank strain is inoculated with the inoculum concentration that solid fermentation culture medium is 3% by weight, Temperature 24 DEG C, humidity 80% is fermented 23 days, and wherein every 3 days, stirring once, prepares solid fermentation thing；Described solid fermentation is trained Foster base is that sterilizing 150 min prepares under the conditions of 100 DEG C, and solid medium by every 500 grams of Pericarpium Vitis viniferaes add 360g rice, The solid matrix raw materials such as 240g Semen Tritici aestivi, 160g Semen Maydis and 540g Sorghum vulgare Pers. prepare；Raw material in described solid fermentation culture medium is by weight The amount ratio than 1:1.3 mixes with water；

(5) solid fermentation thing is after 110 DEG C dry 13 hours, is crushed to below 100 mesh, is packaged to be edible function Property food；Described functional food contains the flavone of 2mg/g butt matter, and the ganoderan of 10mg/g butt matter, 20mg/g are dry The Ganodenic acid of substrate, animal experiment proves, this product has the merits such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor Effect；

Obtained Ganoderma is converted Pericarpium Vitis viniferae solid fermentation thing ovendry power and is broken to below 20 mesh or functional food by C1, adds bacterium The ethanol of the 81% of 15 times of volumes of filament weight, repeatedly extracts 4 times, obtains extracting solution；

Extracting solution obtained by step C1 is merged by C2, is then evaporated to the 1/30 of original volume, adds residual volume 5 times Water, crystallizes 14 hours under the conditions of 4 DEG C, and then sucking filtration dry, pulverize to 100 mesh, i.e. obtains containing flavone and Ganodenic acid Functional food, this food flavone and ganoderic acid content are respectively 57% and 36%.

Obtained Ganoderma mycelium is dried by D1, is then crushed to 20 mesh, adds the distillation of 13 times of volumes of mycelium weight Water, 92 DEG C are repeatedly extracted 4 times, obtain extracting solution；

Extracting solution obtained by step D1 is merged by D2, is then evaporated to the 1/30 of original volume, adds residual volume 1 times 95% ethanol, crystallizes 12 hours under the conditions of 4 DEG C, filters, and filtrate continuously adds 95% ethanol extracting raffinate volume 3.1 times, Crystallizing 12 hours under the conditions of 4 DEG C, then sucking filtration is dried, and i.e. obtains ganoderan, ganoderan purity 42%；

Schematically being described the present invention and embodiment thereof above, this description does not has restricted, actual knot Structure is not limited thereto.So, if those of ordinary skill in the art is enlightened by it, without departing from the invention objective In the case of, design the frame mode similar to this technical scheme and embodiment without creative, all should belong to the present invention's Protection domain.

Claims

1. one kind utilizes Ganoderma to convert the method that Pericarpium Vitis viniferae produces functional food, it is characterised in that described preparation method is with Portugal Grape skin and rice, Semen Tritici aestivi, Semen Maydis, Sorghum vulgare Pers. etc. are solid matrix, with Ganoderma as starting strain, sequentially pass through test tube amplification culture, Liquid submerged culture and seed tank amplification culture, solid fermentation cultivate, dry, pulverize and prepared by the technique such as packaging；Described merit Energy property food contains the flavone of 2～50mg/g butt matter, the ganoderan of 10～100mg/g butt matter, 1～20mg/g butt matter Ganodenic acid, there is the effects such as regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor；Described functional food is permissible Extract its active component flavone, ganoderan and Ganodenic acid, be used for producing treatment reduce blood fat, blood glucose and blood pressure, radioprotective and Medicine or the functional foods such as the tablet of suppression tumor or capsule.

A kind of Ganoderma the most according to claim 1 convert Pericarpium Vitis viniferae produce functional food method, it is characterised in that The Pericarpium Vitis viniferae that Pericarpium Vitis viniferae raw material is Production of Wine factory used or Sucus Vitis viniferae produce and produce the Pericarpium Vitis viniferae that factory is raw；The spirit used Sesame strain include Ganoderma applanatum (Pers. Ex Wallr) Pat. (Ganoderma applanatum), Ganoderma (Ganoderma lucidum) or Ganoderma (Ganoderma sinensis)。

A kind of Ganoderma the most according to claim 1 converts the method that Pericarpium Vitis viniferae produces functional food, it is characterised in that institute State preparation method to comprise the steps:

A kind of Ganoderma the most according to claim 3 converts the method that Pericarpium Vitis viniferae produces functional food, it is characterised in that institute State preparation method specific as follows:

B2 liquid submerged culture: the test tube slant strain obtained by step B1 is cut into 3 × 3 mm fritter strains, picking 3～10 Block is inoculated in the 250mL triangular flask equipped with 20～150mL liquid submerged culture bases, triangular flask rotating speed be 50～200 turns/ Point, under conditions of temperature 20～35 DEG C, 18-86h cultivated by shaking table, makes liquid shaking bottle strain；

B5 solid fermentation thing, after 80～120 DEG C dry 10～24 hours, is crushed to below 100 mesh, is packaged to be and can eat Functional food.

A kind of Ganoderma the most according to claim 4 converts the method that Pericarpium Vitis viniferae produces functional food, it is characterised in that step Liquid submerged culture base described in rapid B2 is that sterilizing 10～60 min prepares under the conditions of 100～130 DEG C, and every liter of liquid shaking bottle Containing wheat bran 5～20g in culture medium, the raw material of Pericarpium Vitis viniferae 5～20g, the base of seed tank amplification culture described in step B3 is 100 ～sterilizing 10～60 min prepares under the conditions of 130 DEG C, and containing wheat bran 5～20g, Pericarpium Vitis viniferae 5 in every liter of liquid submerged culture base ～the raw material of 20g, solid fermentation culture medium described in step B4 is sterilizing 90～360 min system under the conditions of 100～130 DEG C Obtain, and every 500 grams of Pericarpium Vitis viniferaes add 200～400g rice, 100～300g Semen Tritici aestivi, 100～300g Semen Maydis and 700～300g height The solid matrix raw materials such as fine strain of millet.

A kind of Ganoderma the most according to claim 7 converts the method that Pericarpium Vitis viniferae produces functional food, it is characterised in that institute The ratio stating the 1:0.6～1.5 by weight of the solid matrix in solid fermentation culture medium mixes with water.

A kind of Ganoderma the most according to claim 1 converts the method that Pericarpium Vitis viniferae produces functional food, it is characterised in that step Convert from Ganoderma described in rapid A4, A5 or step B4, B5 and the functional food that Pericarpium Vitis viniferae produces extracts flavone and Ganodenic acid Method comprises the steps:

A kind of Ganoderma the most according to claim 1 converts the method that Pericarpium Vitis viniferae produces functional food, it is characterised in that step Convert, from Ganoderma, the method extracting ganoderan the functional food that Pericarpium Vitis viniferae produces described in rapid A4, A5 or step B4, B5 Comprise the steps:

9. converting, according to a kind of Ganoderma described in right 1, the method that Pericarpium Vitis viniferae produces functional food, step C2 and D2 add Ganoderan that work obtains or Ganodenic acid and flavone, can be processed into tablet and glue according to conventional tablet or capsule preparation method Capsule, the tablet being processed into and capsule have regulation blood fat, blood glucose and blood pressure, radioprotective and suppression tumor.