CN107811279A - A kind of method of medical edible fungal fermentation green peel of walnut and its fermented product and application - Google Patents
A kind of method of medical edible fungal fermentation green peel of walnut and its fermented product and application Download PDFInfo
- Publication number
- CN107811279A CN107811279A CN201710871252.5A CN201710871252A CN107811279A CN 107811279 A CN107811279 A CN 107811279A CN 201710871252 A CN201710871252 A CN 201710871252A CN 107811279 A CN107811279 A CN 107811279A
- Authority
- CN
- China
- Prior art keywords
- walnut
- green peel
- fermentation
- fungi
- edible fungal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000009496 Juglans regia Nutrition 0.000 title claims abstract description 121
- 235000020234 walnut Nutrition 0.000 title claims abstract description 119
- 238000000855 fermentation Methods 0.000 title claims abstract description 72
- 230000004151 fermentation Effects 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000002538 fungal effect Effects 0.000 title claims abstract description 29
- 240000007049 Juglans regia Species 0.000 title description 93
- 241000233866 Fungi Species 0.000 claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 230000035622 drinking Effects 0.000 claims abstract description 24
- 235000008216 herbs Nutrition 0.000 claims abstract description 24
- 230000010354 integration Effects 0.000 claims abstract description 24
- 239000003814 drug Substances 0.000 claims abstract description 12
- 239000002537 cosmetic Substances 0.000 claims abstract description 7
- 235000013305 food Nutrition 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 235000013402 health food Nutrition 0.000 claims abstract description 4
- 241000758789 Juglans Species 0.000 claims abstract 28
- 230000001580 bacterial effect Effects 0.000 claims description 41
- 240000008397 Ganoderma lucidum Species 0.000 claims description 23
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims description 23
- 241000222684 Grifola Species 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 244000197580 Poria cocos Species 0.000 claims description 17
- 235000008599 Poria cocos Nutrition 0.000 claims description 17
- 230000001954 sterilising effect Effects 0.000 claims description 15
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 11
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 claims description 11
- 239000007952 growth promoter Substances 0.000 claims description 9
- 244000060234 Gmelina philippensis Species 0.000 claims description 8
- 241000577951 Hydnum Species 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 244000061456 Solanum tuberosum Species 0.000 claims description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000007836 KH2PO4 Substances 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 230000009514 concussion Effects 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 238000005057 refrigeration Methods 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 2
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 2
- 240000006365 Vitis vinifera Species 0.000 claims description 2
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 2
- 230000007760 free radical scavenging Effects 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- 239000013028 medium composition Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 2
- 238000011218 seed culture Methods 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 8
- 229920001282 polysaccharide Polymers 0.000 abstract description 6
- 239000005017 polysaccharide Substances 0.000 abstract description 6
- 150000004676 glycans Chemical class 0.000 abstract description 5
- 239000002207 metabolite Substances 0.000 abstract description 4
- 229920002678 cellulose Polymers 0.000 abstract description 3
- 239000001913 cellulose Substances 0.000 abstract description 3
- 229930003935 flavonoid Natural products 0.000 abstract description 3
- 235000017173 flavonoids Nutrition 0.000 abstract description 3
- 150000002215 flavonoids Chemical class 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 229930192627 Naphthoquinone Chemical class 0.000 abstract description 2
- ZONYXWQDUYMKFB-UHFFFAOYSA-N SJ000286395 Natural products O1C2=CC=CC=C2C(=O)CC1C1=CC=CC=C1 ZONYXWQDUYMKFB-UHFFFAOYSA-N 0.000 abstract description 2
- 229920002472 Starch Polymers 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000000975 bioactive effect Effects 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 abstract description 2
- 229930003949 flavanone Natural products 0.000 abstract description 2
- 150000002207 flavanone derivatives Chemical class 0.000 abstract description 2
- 235000011981 flavanones Nutrition 0.000 abstract description 2
- 150000002791 naphthoquinones Chemical class 0.000 abstract description 2
- 150000002989 phenols Chemical class 0.000 abstract description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 abstract description 2
- 235000013824 polyphenols Nutrition 0.000 abstract description 2
- 229930182490 saponin Natural products 0.000 abstract description 2
- 235000017709 saponins Nutrition 0.000 abstract description 2
- 150000007949 saponins Chemical class 0.000 abstract description 2
- 150000003384 small molecules Chemical class 0.000 abstract description 2
- 239000008107 starch Substances 0.000 abstract description 2
- 235000019698 starch Nutrition 0.000 abstract description 2
- 235000010980 cellulose Nutrition 0.000 abstract 1
- 229920005610 lignin Polymers 0.000 abstract 1
- 239000001814 pectin Substances 0.000 abstract 1
- 229920001277 pectin Polymers 0.000 abstract 1
- 235000010987 pectin Nutrition 0.000 abstract 1
- 239000000047 product Substances 0.000 description 23
- 239000012530 fluid Substances 0.000 description 12
- -1 triterpene compound Chemical class 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 235000015097 nutrients Nutrition 0.000 description 10
- 239000003963 antioxidant agent Substances 0.000 description 7
- 230000003078 antioxidant effect Effects 0.000 description 7
- 235000006708 antioxidants Nutrition 0.000 description 7
- 238000011081 inoculation Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000000259 anti-tumor effect Effects 0.000 description 5
- 238000001816 cooling Methods 0.000 description 5
- 239000010899 old newspaper Substances 0.000 description 5
- 229920000742 Cotton Polymers 0.000 description 4
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 4
- 241000018646 Pinus brutia Species 0.000 description 4
- 235000011613 Pinus brutia Nutrition 0.000 description 4
- 241000222341 Polyporaceae Species 0.000 description 4
- 235000006040 Prunus persica var persica Nutrition 0.000 description 4
- 230000003064 anti-oxidating effect Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000002218 hypoglycaemic effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 208000001953 Hypotension Diseases 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 208000021822 hypotensive Diseases 0.000 description 3
- 230000001077 hypotensive effect Effects 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 230000001151 other effect Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 241000222382 Agaricomycotina Species 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 240000005809 Prunus persica Species 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
- 150000003797 alkaloid derivatives Chemical class 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000003026 anti-oxygenic effect Effects 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 235000010290 biphenyl Nutrition 0.000 description 2
- 239000004305 biphenyl Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 description 2
- 239000011714 flavin adenine dinucleotide Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- KQPYUDDGWXQXHS-UHFFFAOYSA-N juglone Chemical compound O=C1C=CC(=O)C2=C1C=CC=C2O KQPYUDDGWXQXHS-UHFFFAOYSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 230000001932 seasonal effect Effects 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- 241000221198 Basidiomycota Species 0.000 description 1
- 235000003932 Betula Nutrition 0.000 description 1
- 241000219429 Betula Species 0.000 description 1
- 235000018185 Betula X alpestris Nutrition 0.000 description 1
- 235000018212 Betula X uliginosa Nutrition 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 244000089742 Citrus aurantifolia Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000222356 Coriolus Species 0.000 description 1
- YPZRHBJKEMOYQH-UYBVJOGSSA-N FADH2 Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1COP(O)(=O)OP(O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C(NC(=O)NC2=O)=C2NC2=C1C=C(C)C(C)=C2 YPZRHBJKEMOYQH-UYBVJOGSSA-N 0.000 description 1
- 240000000588 Hericium erinaceus Species 0.000 description 1
- 235000007328 Hericium erinaceus Nutrition 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 244000246386 Mentha pulegium Species 0.000 description 1
- 235000016257 Mentha pulegium Nutrition 0.000 description 1
- 235000004357 Mentha x piperita Nutrition 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- 241000123335 Piptoporus Species 0.000 description 1
- 241000222383 Polyporales Species 0.000 description 1
- 241000222640 Polyporus Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 241000576755 Sclerotia Species 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 206010047623 Vitamin C deficiency Diseases 0.000 description 1
- 241001314279 Zoopagales Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003679 aging effect Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000767 anti-ulcer Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- VYTBDSUNRJYVHL-UHFFFAOYSA-N beta-Hydrojuglone Natural products O=C1CCC(=O)C2=C1C=CC=C2O VYTBDSUNRJYVHL-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000015190 carrot juice Nutrition 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000002024 ethyl acetate extract Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 description 1
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 description 1
- 238000002376 fluorescence recovery after photobleaching Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000001050 hortel pimenta Nutrition 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 125000000717 hydrazino group Chemical group [H]N([*])N([H])[H] 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 229950006238 nadide Drugs 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- OVARTBFNCCXQKS-UHFFFAOYSA-N propan-2-one;hydrate Chemical compound O.CC(C)=O OVARTBFNCCXQKS-UHFFFAOYSA-N 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 150000004053 quinones Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 208000010233 scurvy Diseases 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- RXHIKAIVEMAPRU-JRIGQVHBSA-N sequiterpene Natural products C1=C(C)[C@@H](OC(C)=O)[C@H](O)[C@@]2(O)[C@H](C)CC[C@@H](C(C)=C)[C@H]21 RXHIKAIVEMAPRU-JRIGQVHBSA-N 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/52—Juglandaceae (Walnut family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The present invention discloses method and its fermented product and the application of a kind of medical edible fungal fermentation green peel of walnut.The method of the medical edible fungal fermentation green peel of walnut includes green peel of walnut preparation, actication of culture, liquid seeds culture, fungi fermentation, fermentation termination determination step.Method of the fermented product through medical edible fungal fermentation green peel of walnut is prepared.The application is that prepared by medical edible fungal fermentation green peel of walnut fermented product into food, health food, cosmetics and/or medicine.The present invention is fermented using the macro fungi of integration of drinking and medicinal herbs to green peel of walnut, there is the enzyme system of the materials such as degraded cellulose, pectin, starch using fungi, the cellulose of green peel of walnut, lignin etc. are decomposed into small molecule carbon source, the beneficial activity compositions such as phenols in green peel of walnut, flavanone, naphthoquinones class are remained again, it also add fungus metabolite, so that fermented product contains the bioactive substances such as abundant fungi polysaccharide, saponins, Polyphenols, flavonoids, have wide range of applications.
Description
Technical field
The invention belongs to technical field of bioengineering, and in particular to a kind of method of medical edible fungal fermentation green peel of walnut and
Its fermented product and application.
Background technology
The fungi of integration of drinking and medicinal herbs is due to rich in terpenoid (sequiterpene, diterpene-kind compound and triterpene compound, four
Terpenoid), polysaccharide compound, polypeptide, alkaloid, phytosterin compound, nucleoside compound isoreactivity material,
There is antibacterial, antiviral, anticancer, anti-oxidant, raising immunity, hypotensive, hypoglycemic, norcholesterol and other effects mostly.Due to
Restricted by physiological status particularity and complexity and external environment condition, cause the fungi of integration of drinking and medicinal herbs to form son in nature
Entity is rare, and particularly being formed can be needed for many years with fructification.And artificial cultivation is extremely difficult, condition of culture is harsh, and grows week
Phase is up to, it is difficult to meets the market needs increasingly expanded.There is now a variety of macro fungis can be by liquid fermentation, in the short time
Substantial amounts of mycelium is obtained in (such as 5~45d), and its a variety of medicinal ingredient is included in zymotic fluid.
Green peel of walnut (walnut green husk) also known as the English walnut Green Dragon, it is the exocarp of walnut, first recorded in《Kai Baoben
Grass》,《Compendium of Materia Medica》The english walnut exocarp of record is bitter, nontoxic.Modern pharmacological research shows that green peel of walnut has antitumor, town
Bitterly, anti-inflammation and anti-oxidant isoreactivity.Has record among the people, green rind walnut, which is steep in wine, the function for the treatment of stomach trouble, also someone's core
Peach rascal treating cancer.Green peel of walnut is as anticancer traditional popular prescription, historical origin.White peach decoction is secret prescription handed down in the family from generation to generation among the people, Zeng Wei
Many patients release slight illness, and green peel of walnut is exactly one of main component in white peach decoction.In proved recipe of traditional Chinese medicine, green peel of walnut
Available for illnesss such as treatment pruitus, sore swell and ache curative, leucoderma.
The research and development of vegetable source natural antioxidant turn into current functional food and additive agent field and cosmetics
The important development direction of raw material, the stabilization of its antioxygenic property directly affect oxidation resistant effect.Green peel of walnut is rich in a variety of
Active material, there is significant anti-oxidation efficacy.But after Walnut Fruit harvesting a large amount of rascal turn into dumping field,
Head or limes marginis, can cause serious environmental pollution, if it be used, can not only reduce environmental pollution, can also increase
Orchard worker is added to take in.
Grifola frondosus (Grifolarondosa (Dicks.：Fr.) S.F.Gray) it is integration of drinking and medicinal herbs Tan bacterium, category basidiomycetes is sub-
Door, Hymenomycetes, Aphyllophorales, Polyporaceae, set flower Pseudomonas.Rich in the functional components such as polysaccharide, vitamin, trace element, energy
Prevent leucoderma, anaemia, scurvy, while have antitumor, hypoglycemic and other effects, modern medicine further proves, moreover it is possible to be anti-
Only artery sclerosis, raising immunity, anti-ageing a variety of effects of waiting for a long time.
Rainbow conk or Corilus versicolor Quel. (CorilusversicolorQuel.), are subordinate to Basidiomycotina, Hymenomycetes, non-pleat
Zoopagales, Polyporaceae, rainbow conk category or Coriolus Qu61.Rainbow conk is widely used in controlling for the diseases such as cancer and immune deficiency in China
Treat, be considered as god's medicine of curing the disease by among the people.Research shows that coriolan has significant antioxidation activity, free to superoxide anion
Base and DPPH free radicals have significant scavenging action.
Hedgehog hydnum (Hericium erinaceus (RullexF.) Pers.) is integration of drinking and medicinal herbs Tan bacterium, has antiulcer, anti-inflammatory
Disease, antitumor, anti-aging, antifatigue, raising body's hypoxia tolerance, increase myocardial blood output quantity, acceleration body blood follow
The effect such as ring, hypoglycemic, liver protecting and reducing blood lipid, hypotensive.
Dome ganoderma lucidum is also known as white sesame (Fuscoporiapunctata (Fr.) Cum), also known as birch stripping pore bacterium, Polyporaceae,
Piptoporus, belong to the obligate domestomycetes of Betula trees, cause xylem formed brown rot, be distributed in China Heilungkiang, Jilin,
The areas such as Liaoning, the Inner Mongol, there is important practical value and medical value.
Poria cocos (Poria cocos) is under the jurisdiction of Eumycota, is integration of drinking and medicinal herbs Tan bacterium.Poria cocos is On Polyporaceae Poria cocos
Dry sclerotia, there is the effect such as diuresis, calmness, antitumor, the anti-dirty tissue of protection, the enhancing rhythm of the heart.
Red ganoderma lucidum (Ganoderma lucidium) is a kind of fungi of integration of drinking and medicinal herbs, the traditional Chinese medical science is regarded to strengthen the body resistance to consolidate the constitution,
Strengthen immunologic function and improve Abwehrkraft des Koepers, there is hypoglycemic, hypotensive, neurasthenia is antitumor, protecting liver and detoxication, resists
Aging and other effects.
The patent of Application No. 2010105338675 discloses a kind of green peel of walnut, the production method of pine needle fermented wine.Will
Green peel of walnut mashing after sterilizing, pine needle defibrination, is allocated, heated, filtering to take its filtrate, sent out using grape wine dry ferment in proportion
Ferment obtains original wine, and through pouring in down a chimney, after fermentation, ageing be made.The comprehensive utilization of green peel of walnut and pine needle is realized, by using walnut
The beneficiating ingredient of rascal and pine needle, the effect of reaching physical fitness.
The patent of Application No. 2010101386646 discloses a kind of method that growing and blacking hair agent is prepared using green peel of walnut.
Hair tonic is prepared using green peel of walnut using one kind of " juglone " material contained by green peel of walnut and other materials compatibility synthesis
The method of blackening agent, to reach the medical functions of growing and blacking hair.
Application No. 201310195534X patents disclose a kind of walnut peel extract and preparation method thereof, Yi Jiqi
The measure of antioxidation in vitro effect.After green peel of walnut dries pulverizing sewage methanol will be used to extract, then dissolved with acetone-water with stone
Oily ether, chloroform, ethyl acetate, extracting n-butyl alcohol, obtain ethyl acetate extract and used as antioxidant.
The patent of Application No. 2011100208010 discloses a kind of new Poria cocos bacterial strain and its liquid fermentation mode, through hair
The Poria cocos of ferment, the yield of mycelial yield and exocellular polysaccharide is improved, add Poria cocos effect.
The patent of Application No. 2013104016324 discloses the bacterium after polyporus frondosus fermenting liquor separation mycelium or solid fermentation
After series of process, there is the frondosa fermentation mycelium Thick many candies of extraction filament obvious suppression to make to avian influenza virus
With.
The content of the invention
The first object of the present invention is to provide a kind of simple method, process safety and is not subject to seasonal restrictions, is advantageous to ring
The method of the medical edible fungal fermentation green peel of walnut of border protection；Second purpose is to provide the fermentation that a kind of first mesh is prepared
Product；3rd purpose is to provide a kind of application of the fermented product of second purpose.
What the first object of the present invention was realized in：Including green peel of walnut preparation, actication of culture, liquid seeds culture,
Fungi fermentation, fermentation termination determination step, are comprised the following steps that：
A, prepared by green peel of walnut：The fresh walnut with rascal is cleaned with flowing water, rascal is removed after naturally dry, cuts fritter
It is standby；
B, actication of culture：The integration of drinking and medicinal herbs fungi strain of preservation is transferred on inclined-plane solid medium at 28~30 DEG C
Cultivate 5~10d；
C, liquid seeds culture：Above-mentioned activated integration of drinking and medicinal herbs fungi strain is cut into one piece to be inoculated in equipped with 100mL
In the 300mL triangular flasks of fluid nutrient medium, with 140~150r/min, 7~10d of concussion and cultivate under the conditions of 28~30 DEG C, until
Form macroscopic fructification；
D, fungi fermentation：Take that the green peel of walnut fritter 90g being prepared is placed in 250mL triangular flasks or 180g is placed in 500mL
In triangular flask, by 3:1~10:1 feed liquid mass volume ratio adds growth promoter, by the cultured fungi of step C after sterilizing
Liquid seeds are inoculated into green peel of walnut in the ratio of green peel of walnut weight 10%, and ferment at constant temperature is until forming macroscopic son
Entity and/or bacterium ball, mycelium；
E, fermentation termination judges：Green peel of walnut is sampled daily during fungi fermentation, progress DPPH clearance rates,
Hydroxyl radical free radical clearance rate and reducing power size measurement, are used as fermentation termination when being peaked using reducing power.
What the second object of the present invention was realized in：The medicine that the method for medical edible fungal fermentation green peel of walnut is prepared
Edible fungus fermentation green peel of walnut fermented product.
What the third object of the present invention was realized in：Medical edible fungal ferments green peel of walnut fermented product in preparation food
Application in product, health food, cosmetics and/or medicine.
The present invention has the advantages that compared with prior art：
1) resource utilization is improved come the green peel of walnut that ferments by the fungi of integration of drinking and medicinal herbs, decreases environment dirt
Dye, it is safe, controllable and be not subject to seasonal restrictions to have suffered production process, is produced on a large scale；Manufactured green peel of walnut fermented product has
Beneficial composition is abundant, reducing power is strong, antioxygenic property is good, absorption rate is high, can be used as food, cosmetics and/or medicine addition
Agent, it is nutritious.
2) present invention uses fungi grifola frondosus, Poria cocos, hedgehog hydnum, dome ganoderma lucidum, rainbow conk or the red ganoderma lucidum of integration of drinking and medicinal herbs to new
Fresh green peel of walnut carries out cold fermentation, has the powerful enzyme of the materials such as decomposition of cellulose, starch, protein, lipid using fungi
System, submerged fermentation is carried out to green peel of walnut matrix with fungi and is decomposed into small molecule carbon source, and can retain phenols in green peel of walnut,
The beneficiating ingredients such as flavanone, naphthoquinones class, while some compositions (such as quinones, flavonoids) in green peel of walnut can promote or press down
The growth of fungi processed and the production of metabolite, not only increase material effective component utilization rate, and it is anti-oxidant to add its
Ability, food, health care, cosmetics and field of medicaments can be widely used in as the active ingredient of antioxidant.
3) present invention is with the fungi fermentation green peel of walnut of integration of drinking and medicinal herbs, and metabolite caused by itself is such as in growth for fungi
Fungi polysaccharide, alkaloid, nucleoside compound etc. accumulate, and there is these compounds antibacterial, antiviral, raising immunity etc. to make
With due to green peel of walnut and the metabolite double action of fungi so that the green peel of walnut fermented product of preparation contains abundant
The bioactive substances such as fungi polysaccharide, saponins, Polyphenols, flavonoids.
4) green peel of walnut that uses of the present invention is cheap, wide material sources；Using medium culture and the side of liquid fermentation
Method, harvests the medicinal active ingredient that mycelia and macroscopic fructification obtain fungi, thus with the cycle it is short, save labour,
Cost is low and small by external environment influence.
Brief description of the drawings
Fig. 1 is embodiment 1 and the determination oxidative result of reference examples different strain green peel of walnut fermented product；
In figure：DPPH -1,1- diphenyl -2- trinitrophenyl-hydrazines, alias 1,1- diphenyl -2- hardships diazanyls (free radical)；
OH-hydroxyl radical free radical；Reducing power-go back protohydrogen, including NADH (reduced Coenzyme II), NADPH (reduced coenzyme Ⅰ), FADH
(flavin adenine dinucleotide (FAD)).
Embodiment
The present invention is further illustrated with reference to the accompanying drawings and examples, but the present invention is not subject in any way
Limitation, based on present invention teach that any changes and modifications made, belong to protection scope of the present invention.
The method of the medical edible fungal fermentation green peel of walnut of the present invention, including green peel of walnut preparation, actication of culture, liquid strain
Sub- culture, fungi fermentation, fermentation termination determination step, are comprised the following steps that：
A, prepared by green peel of walnut：The fresh walnut with rascal is cleaned with flowing water, rascal is removed after naturally dry, cuts fritter
It is standby；
B, actication of culture：The integration of drinking and medicinal herbs fungi strain of preservation is transferred on inclined-plane solid medium at 28~30 DEG C
Cultivate 5~10d；
C, liquid seeds culture：Above-mentioned activated integration of drinking and medicinal herbs fungi strain is cut into one piece to be inoculated in equipped with 100mL
In the 300mL triangular flasks of fluid nutrient medium, with 140~150r/min, 7~10d of concussion and cultivate under the conditions of 28~30 DEG C, until
Form macroscopic fructification；
D, fungi fermentation：Take that the green peel of walnut fritter 90g being prepared is placed in 250mL triangular flasks or 180g is placed in 500mL
In triangular flask, by 3:1 feed liquid mass volume ratio adds growth promoter, by the cultured fungi liquid kind of step C after sterilizing
Son is inoculated into green peel of walnut in the ratio of green peel of walnut weight 10%, and ferment at constant temperature is until forming macroscopic fructification
And/or bacterium ball, mycelium；
E, fermentation termination judges：Green peel of walnut is sampled daily during fungi fermentation, progress DPPH clearance rates,
Hydroxyl radical free radical clearance rate and reducing power size measurement, are used as fermentation termination when being peaked using reducing power.
The integration of drinking and medicinal herbs fungi for human body will not be caused to be poisoned after edible and can be formed meat or colloid naked eyes it is visible
The fungi of fructification, including grifola frondosus, dome ganoderma lucidum, Poria cocos, rainbow conk, red ganoderma lucidum or hedgehog hydnum.
The grifola frondosus is G2M510 bacterial strains (preserving number GIM5.10), G2M563 bacterial strains (GIM5.63), G2M5164 bacterial strains
(GIM5.164), the dome ganoderma lucidum is G2M56 bacterial strains (GIM5.6), the Poria cocos are G2M5219 bacterial strains (GIM5.219), institute
It is G to state rainbow conk2M5178 bacterial strains (GIM5.178), the red ganoderma lucidum are G2M5250 bacterial strains (GIM5.250), the hedgehog hydnum are
G2M5208 bacterial strains (GIM5.208), above-mentioned bacterial strains are all from Guangdong Province's Culture Collection (GDMCC).
Green peel of walnut is cut into the fritter that length is less than 1cm in the step A.
Green peel of walnut cuts after fritter that to be placed in -20 DEG C of refrigerations through sterilizing standby in the step A.
Inclined-plane solid medium in the step B is PDA slant mediums, and the PDA inclined-plane cultures based component presses matter
Amount percentage includes：Potato 20%, glucose 2%, the water of agar 1.5~2% and surplus.
Strain in the step B is inoculated at 1/3 above the surface bottom of inclined-plane solid medium, and the strain is oblique
Culture to mycelia covers with inclined-plane at 28 DEG C on the solid medium of face.
Fluid nutrient medium in the step C is PDA liquid medium, and the PDA liquid medium composition presses quality hundred
Ratio is divided to include：The water of potato 20%, glucose 2% and surplus.
Growth promoter composition in the D steps includes by mass percentage：0.1% MgSO4, 0.2%
KH2PO4, 0.13% peptone and the water of surplus.
The condition of green peel of walnut ferment at constant temperature is in the D steps：28~30 DEG C of thermostat temperature, fermentation time be 7~
14d。
Sterilizing is to cover bottleneck simultaneously with eight layers of gauze after green peel of walnut and growth promoter are placed in triangular flask in the D steps
Wrapping, external application old newspaper parcel bottleneck, sterilize 20min in 121 DEG C, then natural cooling.
The method of the medical edible fungal fermentation green peel of walnut of the present invention also includes freeze-drying step, the freeze-drying step
Suddenly be up to fermentation termination fermented product crushed after vacuum freeze drying obtain medical edible fungal fermentation green peel of walnut hair
Ferment product pulvis.
The fermented product includes fermented- green peel of walnut, hypha,hyphae and fructification and/or zymotic fluid.
The medical edible fungal fermentation green peel of walnut that the method for the medical edible fungal fermentation green peel of walnut of the present invention is prepared
Fermented product.
The present invention medical edible fungal fermentation green peel of walnut fermented product prepare food, health food, cosmetics and/or
Application in medicine.
Embodiment 1
S100：The fresh walnut with rascal is cleaned with flowing water, naturally dry, removes rascal, is cut into 1 × 1cm sizes and is accounted for
80% fritter, it is standby to put -20 DEG C of refrigerations.
S200：By the integration of drinking and medicinal herbs fungi strain grifola frondosus G of preservation2M510 bacterial strains (preserving number GIM5.10), grifola frondosus
G2M563 bacterial strains (GIM5.63), grifola frondosus G2M5164 bacterial strains (GIM5.164), dome ganoderma lucidum G2M56 bacterial strains (GIM5.6), Poria cocos
G2M5219 bacterial strains (GIM5.219), rainbow conk G2M5178 bacterial strains (GIM5.178), red ganoderma lucidum G2M5250 bacterial strains (GIM5.250), monkey
Head G2M5208 bacterial strains (GIM5.208) (each strain is all from Guangdong Province Culture Collection GDMCC) are oblique from parent species respectively
Interview Guan Zhongyong inoculation shovels cut 1cm2The mycelia block of size, it is inoculated in respectively above the surface bottom of each PDA slant mediums
At 1/3, at 28 DEG C cultivate 5~10d and cover with inclined-plane to mycelia；PDA inclined-plane culture based components include by mass percentage：
Potato 20%, glucose 2%, the water of agar 2% and surplus.
S300：The integration of drinking and medicinal herbs fungi strain that S200 is activated, 1cm is cut with inoculation shovel2Fungus block be inoculated in and be equipped with
In the 300mL triangular flasks of 100mL fluid nutrient mediums, with 150r/min 7~10d of concussion and cultivate under the conditions of 28~30 DEG C, until
Form macroscopic fructification and bacterium ball.
S400：The green peel of walnut fritter 90g for taking S100 to prepare is placed in 250mL triangular flasks, by 3:1 solid-liquid ratio adds
30mL growth promoters, then cover bottleneck with eight layers of gauze and wrapped up with cotton thread, external application old newspaper parcel bottleneck, in 121 DEG C
Natural cooling after sterilizing 20min, whole process are carried out in aseptic operating platform；By the cultured fungi liquid kinds of S300 after sterilizing
Son takes 9g to be inoculated into green peel of walnut, in 28 DEG C of 7~14d of ferment at constant temperature, until forming macroscopic fructification；Nutrient solution into
Divide includes by mass percentage：0.1% MgSO4, 0.2% KH2PO4, 0.13% peptone and the water of surplus.
S500：Green peel of walnut is sampled daily during fungi fermentation, carries out DPPH clearance rates, hydroxyl radical free radical
Clearance rate and reducing power size measurement, are used as fermentation termination when being peaked using reducing power.Bacterium of the fungi in green peel of walnut
Silk growing state such as table 1.
S600：The fermented products such as green peel of walnut, hypha,hyphae and fructification and the zymotic fluid of fermentation termination are up to through true
Crushed after vacuum freecing-dry, obtain medical edible fungal fermentation green peel of walnut fermented product pulvis.
DPPH clearance rates are pressed in S500《The anti-oxidant spectrum effect relationship research of peppermint medicinal material based on DPPH, FRAP method》, Xu Jing
Crystalline substance etc., Beijing University of Chinese Medicine's journal, 2015,38 (6):405-410 methods describeds are measured；Hydroxyl radical free radical (OH) is removed
Rate is pressed《The research of purple carrot juice antioxidation activity in vitro》, the brave spring is gorgeous etc., and China brewages, 2015,34 (1)：Described in 85-88
Method is measured；Reducing power is pressed《The changing rule of oxidation resistance in Tea Fungus Fermentation》, Zhu Xiaoqing etc., production and section
Grind experience, 2010,36 (11)：111-114 methods describeds are measured.
Mycelial growth situation of the fungi of table 1 in green peel of walnut
Embodiment 2
S100：The fresh walnut with rascal is cleaned with flowing water, naturally dry, removes rascal, is cut into 1 × 0.5cm sizes and is accounted for
80% fritter, it is standby to put -20 DEG C of refrigerations.
S200：By the integration of drinking and medicinal herbs fungi strain grifola frondosus G of preservation2M510 bacterial strains (preserving number GIM5.10), grifola frondosus
G2M563 bacterial strains (GIM5.63), grifola frondosus G2M5164 bacterial strains (GIM5.164), dome ganoderma lucidum G2M56 bacterial strains (GIM5.6), Poria cocos
G2M5219 bacterial strains (GIM5.219), rainbow conk G2M5178 bacterial strains (GIM5.178), red ganoderma lucidum G2M5250 bacterial strains (GIM5.250) are (each
Strain is all from Guangdong Province's Culture Collection) cut 1cm with inoculation shovel from parent species slant tube respectively2Size
Mycelia block, is inoculated at 1/3 above the surface bottom of each PDA slant mediums respectively, and 5~10d is cultivated at 30 DEG C to mycelia
Cover with inclined-plane；PDA inclined-plane culture based components include by mass percentage：Potato 20%, glucose 2%, agar 1.5%
And the water of surplus.
S300：The integration of drinking and medicinal herbs fungi strain that S200 is activated, 1cm is cut with inoculation shovel2Fungus block be inoculated in and be equipped with
In the 300mL triangular flasks of 100mL fluid nutrient mediums, with 140r/min 7~10d of concussion and cultivate under the conditions of 30 DEG C, until being formed
Macroscopic fructification.
S400：The green peel of walnut fritter 90g for taking S100 to prepare is placed in 250mL triangular flasks, by 5:1 solid-liquid ratio adds
18mL growth promoters, then cover bottleneck with eight layers of gauze and wrapped up with cotton thread, external application old newspaper parcel bottleneck, in 121 DEG C
Natural cooling after sterilizing 20min, whole process are carried out in aseptic operating platform；By the cultured fungi liquid kinds of S300 after sterilizing
Son takes 9g to be inoculated into green peel of walnut, in 30 DEG C of 7~14d of ferment at constant temperature, until forming macroscopic fructification；Nutrient solution into
Divide includes by mass percentage：0.1% MgSO4, 0.2% KH2PO4, 0.13% peptone and the water of surplus.
S500：Green peel of walnut is sampled daily during fungi fermentation, it is clear to carry out DPPH as described in Example 1
Except rate, hydroxyl radical free radical clearance rate and reducing power size measurement, fermentation termination is used as when being peaked using reducing power.Fungi exists
Mycelial growth situation such as table 2 in green peel of walnut.
S600：The fermented products such as green peel of walnut, hypha,hyphae and fructification and the zymotic fluid of fermentation termination are up to through true
Crushed after vacuum freecing-dry, obtain medical edible fungal fermentation green peel of walnut fermented product pulvis.
Mycelial growth situation of the fungi of table 2 in green peel of walnut
| 1d | 3d | 5d | 7d | 9d | 11d | 13d | 15d | |
| Grifola frondosus G2M510 | — | — | — | + | ++ | ++ | +++ | +++ |
| Grifola frondosus G2M563 | — | — | — | + | ++ | ++ | ++ | +++ |
| Grifola frondosus G2M5164 | — | — | — | + | ++ | ++ | ++ | +++ |
| Dome ganoderma lucidum G2M56 | — | — | + | ++ | ++ | ++ | +++ | +++ |
| Poria cocos G2M5219 | — | — | + | ++ | ++ | +++ | +++ | +++ |
| Rainbow conk G2M5178 | — | — | + | +++ | +++ | +++ | +++ | +++ |
| Red ganoderma lucidum G2M5250 | — | — | + | ++ | ++ | +++ | +++ | +++ |
| Hedgehog hydnum G2M5208 | — | — | + | ++ | ++ | ++ | +++ | +++ |
Embodiment 3
S100：The fresh walnut with rascal is cleaned with flowing water, naturally dry, removes rascal, is cut into 0.8 × 0.8cm sizes
80% fritter is accounted for, it is standby to put -20 DEG C of refrigerations.
S200：By the integration of drinking and medicinal herbs fungi strain grifola frondosus G of preservation2M510 bacterial strains (preserving number GIM5.10), grifola frondosus
G2M563 bacterial strains (GIM5.63), grifola frondosus G2M5164 bacterial strains (GIM5.164), dome ganoderma lucidum G2M56 bacterial strains (GIM5.6), Poria cocos
G2M5219 bacterial strains (GIM5.219), rainbow conk G2M5178 bacterial strains (GIM5.178), red ganoderma lucidum G2M5250 bacterial strains (GIM5.250) are (each
Strain is all from Guangdong Province's Culture Collection) cut 1cm with inoculation shovel from parent species slant tube respectively2Size
Mycelia block, is inoculated at 1/3 above the surface bottom of each PDA slant mediums respectively, and 5~10d is cultivated at 28 DEG C to mycelia
Cover with inclined-plane；PDA inclined-plane culture based components include by mass percentage：Potato 20%, glucose 2%, agar 2% and
The water of surplus.
S300：The integration of drinking and medicinal herbs fungi strain that S200 is activated, 1cm is cut with inoculation shovel2Fungus block be inoculated in and be equipped with
In the 300mL triangular flasks of 100mL fluid nutrient mediums, with 150r/min 7~10d of concussion and cultivate under the conditions of 28 DEG C, until being formed
Macroscopic fructification.
S400：The green peel of walnut fritter 180g for taking S100 to prepare is placed in 500mL triangular flasks, by 10:1 solid-liquid ratio adds
18mL growth promoters, then cover bottleneck with eight layers of gauze and wrapped up with cotton thread, external application old newspaper parcel bottleneck, in 121 DEG C
Natural cooling after sterilizing 20min, whole process are carried out in aseptic operating platform；By the cultured fungi liquid kinds of S300 after sterilizing
Son takes 9g to be inoculated into green peel of walnut, in 28 DEG C of 7~14d of ferment at constant temperature, until forming macroscopic fructification；Nutrient solution into
Divide includes by mass percentage：0.1% MgSO4, 0.2% KH2PO4, 0.13% peptone and the water of surplus.
S500：Green peel of walnut is sampled daily during fungi fermentation, carried out as described in Example 1
DPPH clearance rates, hydroxyl radical free radical clearance rate and reducing power size measurement, are used as fermentation termination when being peaked using reducing power.
Mycelial growth situation of the fungi in green peel of walnut such as table 3.
S600：The fermented products such as green peel of walnut, hypha,hyphae and fructification and the zymotic fluid of fermentation termination are up to through true
Crushed after vacuum freecing-dry, obtain medical edible fungal fermentation green peel of walnut fermented product pulvis.
Mycelial growth situation of the fungi of table 3 in green peel of walnut
| 1d | 3d | 5d | 7d | 9d | 11d | 13d | 15d | |
| Grifola frondosus G2M510 | — | — | + | + | ++ | ++ | +++ | +++ |
| Grifola frondosus G2M563 | — | — | + | + | ++ | ++ | ++ | +++ |
| Grifola frondosus G2M5164 | — | — | + | + | ++ | ++ | ++ | +++ |
| Dome ganoderma lucidum G2M56 | — | — | + | ++ | ++ | ++ | +++ | +++ |
| Poria cocos G2M5219 | — | — | + | ++ | ++ | +++ | +++ | +++ |
| Rainbow conk G2M5178 | — | — | + | ++ | +++ | +++ | +++ | +++ |
| Red ganoderma lucidum G2M5250 | — | — | + | ++ | ++ | +++ | +++ | +++ |
| Hedgehog hydnum G2M5208 | — | — | + | ++ | ++ | ++ | +++ | +++ |
Reference examples
S100：The fresh walnut with rascal is cleaned with flowing water, naturally dry, removes rascal, is cut into 1 × 1cm sizes and is accounted for
80% fritter, it is standby to put -20 DEG C of refrigerations.
S200：The green peel of walnut fritter 90g for taking S100 to prepare is placed in 250mL triangular flasks, by 3:1 (g/mL) solid-liquid ratio
30mL growth promoters are added, then bottleneck is covered with eight layers of gauze and is wrapped up with cotton thread, external application old newspaper parcel bottleneck, in
Natural cooling after 121 DEG C of sterilizing 20min, whole process are carried out in aseptic operating platform；After sterilizing 9g sterilized waters will be taken to be inoculated into
In green peel of walnut, in 28 DEG C of 7~14d of ferment at constant temperature；Nutrient composition includes by mass percentage：0.1% MgSO4, 0.2%
KH2PO4, 0.13% peptone and the water of surplus.
S300：Measure DPPH clearance rates, hydroxyl are sampled to the green peel of walnut for the 14d that fermented as described in Example 1
Free radical scavenging activity and reducing power size.
S400：The fermented products such as the green peel of walnut for the 14d that ferments and zymotic fluid are crushed after vacuum freeze drying, obtain core
Peach rascal fermented product pulvis.
Claims (10)
- A kind of 1. method of medical edible fungal fermentation green peel of walnut, it is characterised in that including green peel of walnut preparation, actication of culture, liquid Body seed culture, fungi fermentation, fermentation termination determination step, are comprised the following steps that：A, prepared by green peel of walnut：The fresh walnut with rascal is cleaned with flowing water, rascal is removed after naturally dry, it is standby to cut fritter With；B, actication of culture：The integration of drinking and medicinal herbs fungi strain of preservation is transferred on inclined-plane solid medium and cultivated at 28~30 DEG C 5~10d；C, liquid seeds culture：Above-mentioned activated integration of drinking and medicinal herbs fungi strain is cut into one piece to be inoculated in equipped with 100mL liquid In the 300mL triangular flasks of culture medium, with 140~150r/min, 7~10d of concussion and cultivate under the conditions of 28~30 DEG C, until being formed Macroscopic fructification；D, fungi fermentation：Take that the green peel of walnut fritter 90g being prepared is placed in 250mL triangular flasks or 180g is placed in 500mL triangles In bottle, by 3:1~10:1 feed liquid mass volume ratio adds growth promoter, by the cultured fungi liquid of step C after sterilizing Seed is inoculated into green peel of walnut in the ratio of green peel of walnut weight 10%, and ferment at constant temperature is until forming macroscopic fructification And/or bacterium ball, mycelium；E, fermentation termination judges：Green peel of walnut is sampled daily during fungi fermentation, carries out DPPH clearance rates, hydroxyl Free radical scavenging activity and reducing power size measurement, are used as fermentation termination when being peaked using reducing power.
- 2. the method for medical edible fungal fermentation green peel of walnut according to claim 1, it is characterised in that the integration of drinking and medicinal herbs is true Bacterium is grifola frondosus, dome ganoderma lucidum, Poria cocos, rainbow conk, red ganoderma lucidum or hedgehog hydnum.
- 3. the method for medical edible fungal fermentation green peel of walnut according to claim 2, it is characterised in that the grifola frondosus is G2M510 bacterial strains, G2M563 bacterial strains, G2M5164 bacterial strains, the dome ganoderma lucidum are G2M56 bacterial strains, the Poria cocos are G2M5219 bacterial strains, The rainbow conk is G2M5178 bacterial strains, the red ganoderma lucidum are G2M5250 bacterial strains, the hedgehog hydnum are G2M5208 bacterial strains, above-mentioned bacterial strains are equal From Guangdong Province's Culture Collection.
- 4. the method for medical edible fungal fermentation green peel of walnut according to claim 1, it is characterised in that walnut in the step A Rascal cuts after fritter that to be placed in -20 DEG C of refrigerations through sterilizing standby.
- 5. the method for medical edible fungal fermentation green peel of walnut according to claim 1, it is characterised in that oblique in the step B Face solid medium is PDA slant mediums, and the PDA inclined-plane cultures based component includes by mass percentage：Potato 20%, The water of glucose 2%, agar 1.5~2% and surplus.
- 6. the method for medical edible fungal fermentation green peel of walnut according to claim 1, it is characterised in that the liquid in the step C Body culture medium is PDA liquid medium, and the PDA liquid medium composition includes by mass percentage：Potato 20%, grape The water of sugar 2% and surplus.
- 7. the method for medical edible fungal fermentation green peel of walnut according to claim 1, it is characterised in that the life in the D steps Long accelerator composition includes by mass percentage：0.1% MgSO4, 0.2% KH2PO4, 0.13% peptone and surplus Water.
- 8. the method for the green peel of walnut that fermented according to the medical edible fungal of claim 1 or 7, it is characterised in that in the D steps The condition of green peel of walnut ferment at constant temperature is：28~30 DEG C of thermostat temperature, fermentation time are 7~14d.
- 9. the medicine food that a kind of method of medical edible fungal fermentation green peel of walnut described in claim 1 to 8 any one is prepared With fungi fermentation green peel of walnut fermented product.
- 10. medical edible fungal fermentation green peel of walnut fermented product is preparing food, health food, change described in a kind of claim 9 Application in cosmetic and/or medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710871252.5A CN107811279A (en) | 2017-09-25 | 2017-09-25 | A kind of method of medical edible fungal fermentation green peel of walnut and its fermented product and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710871252.5A CN107811279A (en) | 2017-09-25 | 2017-09-25 | A kind of method of medical edible fungal fermentation green peel of walnut and its fermented product and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107811279A true CN107811279A (en) | 2018-03-20 |
Family
ID=61607895
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710871252.5A Pending CN107811279A (en) | 2017-09-25 | 2017-09-25 | A kind of method of medical edible fungal fermentation green peel of walnut and its fermented product and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107811279A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108703909A (en) * | 2018-08-06 | 2018-10-26 | 李媛媛 | A kind of composition with crease-resistant anti-aging effect and its application in cosmetics |
| CN108812423A (en) * | 2018-04-19 | 2018-11-16 | 北京联合大学 | It is a kind of using green peel of walnut fermentation material as the pouity dwelling place padding of matrix |
| CN109010209A (en) * | 2018-09-13 | 2018-12-18 | 合肥巧织纺织科技有限公司 | A kind of wet tissue special suitable for patients with rhinitis |
| CN112826339A (en) * | 2021-01-20 | 2021-05-25 | 河南中立生物科技有限公司 | Low-temperature plant decomposition technology |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200520753A (en) * | 2003-10-14 | 2005-07-01 | Showa Denko Kk | Agent for skin external use containing salt of ascorbic acid derivative, method for stabilizing the agent for skin external use, and stabilizer |
| US20050220744A1 (en) * | 2004-03-31 | 2005-10-06 | Showa Denko K.K. | Polysaccharide/functional compound complex |
| CN100998611A (en) * | 2006-12-28 | 2007-07-18 | 温州大学 | Huishuhua fermentation preparation process using Chinese herb medicine as medium |
| CN102578559A (en) * | 2012-03-07 | 2012-07-18 | 云南省农业科学院生物技术与种质资源研究所 | Medical fungus health care product fermented by taking soybean curb residue as matrix and preparation method of medical fungus health care product |
| CN103875516A (en) * | 2014-03-07 | 2014-06-25 | 福州东星生物技术有限公司 | Method for cultivating lignicolous lucid ganoderma with walnut shells as culture medium |
| CN104288302A (en) * | 2014-10-17 | 2015-01-21 | 湖南裕翔生物科技有限公司 | Method for fermenting Chinese herbs by use of medicinal/edible fungi and application of fermented Chinese herbs |
| CN104830925A (en) * | 2015-05-13 | 2015-08-12 | 浙江大学 | Method for generating aglycon by converting grifola frondosa into soybeam isoflavone glycoside by fermenting |
| CN105347918A (en) * | 2015-11-03 | 2016-02-24 | 梁淑娟 | Method for preparing bio-organic fertilizer from walnut green seedcase |
| CN105708894A (en) * | 2016-02-26 | 2016-06-29 | 云南中医学院 | Method for preparing walnut green husk polyphenol from fresh walnut green husks |
| CN106136127A (en) * | 2016-07-12 | 2016-11-23 | 江苏大学 | A kind of method utilizing Ganoderma to convert Pericarpium Vitis viniferae production functional food |
-
2017
- 2017-09-25 CN CN201710871252.5A patent/CN107811279A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200520753A (en) * | 2003-10-14 | 2005-07-01 | Showa Denko Kk | Agent for skin external use containing salt of ascorbic acid derivative, method for stabilizing the agent for skin external use, and stabilizer |
| US20050220744A1 (en) * | 2004-03-31 | 2005-10-06 | Showa Denko K.K. | Polysaccharide/functional compound complex |
| CN100998611A (en) * | 2006-12-28 | 2007-07-18 | 温州大学 | Huishuhua fermentation preparation process using Chinese herb medicine as medium |
| CN102578559A (en) * | 2012-03-07 | 2012-07-18 | 云南省农业科学院生物技术与种质资源研究所 | Medical fungus health care product fermented by taking soybean curb residue as matrix and preparation method of medical fungus health care product |
| CN103875516A (en) * | 2014-03-07 | 2014-06-25 | 福州东星生物技术有限公司 | Method for cultivating lignicolous lucid ganoderma with walnut shells as culture medium |
| CN104288302A (en) * | 2014-10-17 | 2015-01-21 | 湖南裕翔生物科技有限公司 | Method for fermenting Chinese herbs by use of medicinal/edible fungi and application of fermented Chinese herbs |
| CN104830925A (en) * | 2015-05-13 | 2015-08-12 | 浙江大学 | Method for generating aglycon by converting grifola frondosa into soybeam isoflavone glycoside by fermenting |
| CN105347918A (en) * | 2015-11-03 | 2016-02-24 | 梁淑娟 | Method for preparing bio-organic fertilizer from walnut green seedcase |
| CN105708894A (en) * | 2016-02-26 | 2016-06-29 | 云南中医学院 | Method for preparing walnut green husk polyphenol from fresh walnut green husks |
| CN106136127A (en) * | 2016-07-12 | 2016-11-23 | 江苏大学 | A kind of method utilizing Ganoderma to convert Pericarpium Vitis viniferae production functional food |
Non-Patent Citations (2)
| Title |
|---|
| 杨海龙等: "利用药用真菌深层发酵加工中药" * |
| 耿阳阳;张彦雄;胡译文;何佳丽;: "鲜食核桃研究进展" * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108812423A (en) * | 2018-04-19 | 2018-11-16 | 北京联合大学 | It is a kind of using green peel of walnut fermentation material as the pouity dwelling place padding of matrix |
| CN108703909A (en) * | 2018-08-06 | 2018-10-26 | 李媛媛 | A kind of composition with crease-resistant anti-aging effect and its application in cosmetics |
| CN108703909B (en) * | 2018-08-06 | 2021-01-29 | 思萨生物科技（深圳）有限公司 | Composition with anti-wrinkle and anti-aging effects and application of composition in cosmetics |
| CN109010209A (en) * | 2018-09-13 | 2018-12-18 | 合肥巧织纺织科技有限公司 | A kind of wet tissue special suitable for patients with rhinitis |
| CN112826339A (en) * | 2021-01-20 | 2021-05-25 | 河南中立生物科技有限公司 | Low-temperature plant decomposition technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107629965A (en) | A kind of preparation method of dendrobium candidum fungi fermentation product and its fermented product and application | |
| KR100962587B1 (en) | A method for fermentation of natural plants and herbal medicines, a fermented product prepared therefrom and a paharmaceutical composition, a cosmetic compositon and a food composition comprising the product | |
| KR101358648B1 (en) | Methods of culturing inonotus obliquus, phellinus linteus and ganoderma lucidum using extracts of mushroom | |
| CN107811279A (en) | A kind of method of medical edible fungal fermentation green peel of walnut and its fermented product and application | |
| CN103263448B (en) | Fermentation bacteria used for fermentation pretreatment to improve extraction of Ginkgo biloba L. leaf flavone and application | |
| CN105647974A (en) | Saccharomyces cerevisiae plant fermentation product and application thereof and skin external application preparations | |
| Bijalwan et al. | Insights of medicinal mushroom (Ganoderma lucidum): prospects and potential in India | |
| CN103689550A (en) | Ganoderma lucidum functional food and preparation method thereof | |
| CN103222392A (en) | Production process of ganoderma lucidum mycelia | |
| KR20100104408A (en) | Cordyceps militaris mycellia cultured by using rice and poultry soup for health using the smae | |
| WA et al. | Bioactive potential of some fascinating edible mushrooms Macrolepiota, Russula, Amanita, Vovariella and Grifola as a treasure of multipurpose therapeutic natural product | |
| KR101167295B1 (en) | A cosmetic composition for skin-antiaging effect comprising of oriental medical herbs' extracts fermened and method for preparing the same | |
| TWI422680B (en) | Culture medium for culturing fruiting bodies of antrodia cinnamomea and method for culturing the same | |
| KR101663379B1 (en) | MANUFACTURE OF FERMENTED Allium hookeri FROM YEASTS AND NATURAL ENZYME AND PREPARATION OF COMBINED BEVERAGE FOR QUENCHING THIRST | |
| CN109730315A (en) | A kind of rhizoma polygonati edible mushroom oral solution and preparation method thereof that is antifatigue, improving immunity | |
| CN107602257A (en) | A kind of Medium for Ganoderma lucidum and preparation method thereof | |
| KR102580526B1 (en) | Method of Preparing Soybean Fermented Mycelium | |
| KR20120053564A (en) | Fermented ginseng with ganoderma lucidum beverage manufacturing method and that beverage | |
| KR101769755B1 (en) | A leuconostoc mesenteroides gfc 160704, cosmetic composition including the leuconostoc mesenteroides gfc 160704 or its culture fluid, and manufacturing method of the cosmetic composition | |
| CN104381992A (en) | Eucommia ulmoides olive fungal substance sugar-free food and preparation method thereof | |
| KR20120096410A (en) | A cosmetic composition for skin-antiaging effect comprising of oriental medical herbs' extracts fermened and method for preparing the same | |
| CN112569265A (en) | Preparation method of medicinal fungus powder | |
| CN112119834A (en) | High-activity ganoderma lucidum culture medium | |
| CN105347987A (en) | Culture medium for substitute cultivation of ganoderma lucidum antler | |
| KR20210084788A (en) | Method of growing shiitake mushrooms with improved beta glucan content and yield |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180320 |