CN105837324B - Hypsizigus marmoreus liquid strain medium and planting method - Google Patents
Hypsizigus marmoreus liquid strain medium and planting method Download PDFInfo
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- CN105837324B CN105837324B CN201610186984.6A CN201610186984A CN105837324B CN 105837324 B CN105837324 B CN 105837324B CN 201610186984 A CN201610186984 A CN 201610186984A CN 105837324 B CN105837324 B CN 105837324B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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Abstract
The present invention provides a hypsizigus marmoreus liquid strain medium, which comprises the following components by weight: 74-80 parts of glucose, 14-20 parts of soybean flour, 1-4 parts of potassium dihydrogen phosphate, 1-4 parts of magnesium dulfate, 0.05-0.1 part of a defoaming agent, 2-6 parts of a growth substance and 600-700 parts of water. The invention belongs to the field of edible fungi cultivation technique. The liquid strain medium can fast breed the bacteria, has high coverage of strains, improves vigor and disease resistance of the strain, and has good resistance to cobweb disease.
Description
Technical field
The invention belongs to fungus growing technique field, more particularly to a kind of liquid spawn culture medium and the cultivation of Hypsizygus marmoreuss
Culture method.
Background technology
Hypsizygus marmoreuss (Hypsizygus marmoreus (Peck) H.E.Bigelow), also known as Hypsizygus marmoreus, speckle Hypsizygus marmoreus, Folium Nelumbinis from
Pleat umbrella.Various necessary aminoacid are included in the nutritional labeling of Hypsizygus marmoreuss, its lysine and arginine content are higher than common mushrooms, mouth
Sense is good, with the effects such as diuresis, spleen invigorating.The production of Hypsizygus marmoreuss is all by the way that mycelia, most is inoculated with and then turned out in culture medium
Harvest what the production technology of product was carried out eventually, the poor growth of Hypsizygus marmoreuss, typically using multistage strain breeding of method, i.e., by one-level
Plant (test tube kind), two grades of kinds (triangular flask kind), cultures of three-level kinds (fermentation tank kind) and production bottle to obtain.
The production strain of Hypsizygus marmoreuss includes solid spawn and liquid spawn.Liquid spawn is to use fluid medium, in biology
In fermentation tank, the edible fungus species of the liquid form produced by liquid fermentation technology, liquid spawn is by absorption liquid culture
The nutritional labeling growth of base.Compared with solid spawn, liquid spawn has：The production of hybrid seeds is fast, energetic, shorten cultivation period, cost
The advantages of reduction.
Chinese patent application 200810043506.5 discloses a kind of hypsizigus marmoreus in factory production liquid spawn culture medium, group
Dividing includes soyabean expeller powder, Semen Maydis powder, sucrose, magnesium sulfate, potassium dihydrogen phosphate and defoamer, though the culture medium can realize Hypsizygus marmoreuss bacterium
That what is planted breeds, but the spawn activity cultivated is not ideal enough, and breeding cycle is longer.
The culture medium for cultivating of existing conventional Hypsizygus marmoreuss is made up of following composition：Cotton seed hullss, wood flour, Testa oryzae, bean cake,
Soybean cover, wheat bran, Semen Maydis powder, Calx and fine particle calcium carbonate, wherein cotton seed hullss, wood flour, Testa oryzae and wheat bran are main components.In corn cob
Sugar and crude fiber content it is higher, wherein sugar content about 50%, coarse-fibred content about 30%, due to nutritious and cost compared with
It is low, it is also widely used for the cultivation of edible fungi.
Chinese patent application CN 102432353 discloses a kind of culture medium prescription of Hypsizygus marmoreuss, including wood flour, corn cob,
Testa oryzae, wheat bran, Semen Maydis powder and cotton seed hullss.Chinese patent application CN 102503633 discloses a kind of Hypsizygus marmoreuss compost, including
Rice bran meal, wood flour, wheat bran, corn cob, cotton seed hullss, Gypsum Fibrosum powder and pulverized limestone.
Due to containing some oils and fatss and aromatic substance, being unfavorable for edible mycelial growth and fruit body development in wood flour, because
This generally needs that wood flour is carried out to reuse after defat or lengthy fermentation process, causes process cycle prolongation, cost increase.Cotton
Seed shell mainly originates from the ground such as Xinjiang, originates deficienter, expensive, and pesticide residues are than more serious, therefore, cotton seed hullss are used for
The industrialized production of Hypsizygus marmoreuss does not meet economic and environment-friendly requirement.
Chinese crude drug can produce a large amount of Chinese medicine dregs after extraction, often go out of use.The discharge of Chinese medicine dreg is easily caused
Pollution, containing compositions such as Plant fiber, protein and polysaccharide in Chinese medicine dreg.Prior art includes to the recycling of Chinese medicine dreg
Sewage disposal, it is used as medicine, supports the mode such as mushroom.For scleroid Chinese medicine dreg, such as Radix Notoginseng medicinal residues, Radix Salviae Miltiorrhizae decoction dregs, due to difficulty
To be degraded, its application is limited, lead to not realize making full use of for medicinal residues.
The content of the invention
To solve liquid spawn culture medium and the dissatisfactory problem of culture medium for cultivating present in prior art, inventor
By lot of experiments the liquid spawn culture medium and culture medium for cultivating composition of Hypsizygus marmoreuss are screened and compounded, it is unforeseeable
It was found that：By adding a small amount of growth regulator, strain can be made to breed quickly, strain coverage rate is high, improve spawn activity and disease resistance
Energy.Based on above-mentioned discovery, so as to complete the present invention.
The purpose of the present invention will be further described in detail below reflect and description.
The present invention provides a kind of liquid spawn culture medium of Hypsizygus marmoreuss, including following component and its parts by weight：Glucose
74-80 parts, analysis for soybean powder 14-20 part, potassium dihydrogen phosphate 1-4 parts, magnesium sulfate 1-4 parts, defoamer 0.05-0.1 parts, growth regulator 2-6
Part and water 600-700 parts.
Preferably, the liquid spawn culture medium of the Hypsizygus marmoreuss that the present invention is provided, including following component and its parts by weight：Portugal
650 parts of 78 parts of grape sugar, 16 parts of analysis for soybean powder, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.06 part of defoamer, 4 parts of growth regulator and water.
Preferably, the growth regulator is made up of gastrodine and propylene glycol alginate sodium sulfate with 4-6: 1-3 weight ratio.
Gastrodine is also called Gastrodine, to methylol benzene-β-D pyranglucoside, its chemical formula is：C13H18O7, No. CAS
For：62499-27-8.Gastrodine is to extract to obtain from the dry root block of orchid Rhizoma Gastrodiae, and it has preferably calm and sleeping
Neurasthenia, insomnia, headache syndromes are had mitigation by effect.Propylene glycol alginate sodium sulfate is also called alginic acid the third fat sodium sulfovinate, its change
Formula is：C6H9O7Na, No. CAS is：9005-38-3.Propylene glycol alginate sodium sulfate has anticoagulation, blood fat reducing, improves the work such as microcirculation
With, it is mainly used in ischemic cerebrovascular, hyperlipidemia is also used for, also there is certain curative effect to the disease of cardiovascular system.This
Invention is screened by many experiments, and discovery will be added to by gastrodine and propylene glycol alginate sodium sulfate by the growth regulator that certain weight ratio is constituted
In existing Hypsizygus marmoreuss liquid spawn culture medium, the vigor of Hypsizygus marmoreuss liquid spawn can be significantly improved, improve strain to fermentation
The adaptability of environment and follow-up planting environment.
It is highly preferred that the growth regulator is made up of gastrodine and propylene glycol alginate sodium sulfate by weight 5: 2.
Preferably, the defoamer is polyether modified polysiloxan defoaming agent, is more preferably purchased from Yantai Heng Xin chemical science and technology
The THIX-298 of company limited.
Correspondingly, present invention also offers the preparation method of the liquid spawn culture medium of Hypsizygus marmoreuss, comprises the steps：Claim
Glucose, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate and growth regulator are taken, water is added, adds defoamer, stirring and dissolving, sterilizing to obtain
To the liquid spawn culture medium of Hypsizygus marmoreuss.
Additionally, present invention also offers a kind of cultural method of Hypsizygus marmoreuss, comprises the steps：
S1 prepares liquid spawn culture medium：Weigh 74-80 parts, analysis for soybean powder 14-20 part, potassium dihydrogen phosphate 1-4 parts, magnesium sulfate
1-4 parts and growth regulator 2-6 parts, add water 600-700 parts, add defoamer 0.05-0.1 parts, stirring and dissolving to obtain liquid spawn
Culture medium；
The culture of S2 fermentation tank strains：The liquid spawn culture medium is added in fermentation tank, sterilizing, fermentation jar temperature drop
To 20-24 DEG C, Hypsizygus marmoreuss strain is inoculated in fermentation tank and is cultivated, aerobic culture 5-8 days under the conditions of 20-24 DEG C must ferment
Tank liquid spawn；Fermentor liquid strain Jing check it is pollution-free after, for the cultivation of subsequent production bottle；
S3 prepares culture medium for cultivating：Weigh the corn cob of 40-50 parts by weight, the Fructus Schisandrae Chinensis medicinal residues of 3-8 parts by weight,
The Radix Salviae Miltiorrhizae decoction dregs of 10-20 parts by weight and the Calx of 0.2-1 parts by weight, the temperature that total consumption 2/3 is added while stirring is 8-
15 DEG C of water, stirs 60-70min, is subsequently adding Testa oryzae, the soybean cover of 5-10 parts by weight, the 12-20 weights of 8-12 parts by weight
The wheat bran of amount number and the conch meal of 0.8-2 parts by weight, add remaining water, wet-mixing 30-40min to be planted in wet-mixing
Training culture medium, dress production bottle, sterilizing；
S4 is inoculated with the fermentor liquid strain in production bottle；
S5 mycelia culture, mycelia is covered with and after after-ripening, using steamed bread type mycelium stimulation knife mycelium stimulation fruiting；
S6 fruitings are simultaneously harvested, and obtain Hypsizygus marmoreuss.
Preferably, the culture of the prior Jing one-levels kind of the Hypsizygus marmoreuss strain and two grades of kinds, culture medium can adopt this area
Conventional medium.It is highly preferred that the liquid spawn culture medium culture one-level kind and two grades of kinds of present invention offer can be adopted.
Preferably, the water content of the culture medium for cultivating is that 63-65%, pH are 6.0-6.6.
Preferably, the particle diameter of the corn cob is 2-8mm；The Radix Salviae Miltiorrhizae decoction dregs are crushed to particle diameter Jing after bulking machine is expanded
4-6mm；The Fructus Schisandrae Chinensis medicinal residues it is size-reduced to particle diameter be 1-3mm.
Preferably, the temperature of the mycelia culture is 20-22 DEG C, and humidity is 65-75%, and gas concentration lwevel is 1000-
3500ppm。
Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae are all the widely used medical materials of Chinese herbal medicine industry, usually obtain active component using alcohol extraction and are used as medicine,
It is remaining for medicinal residues.Jing after alcohol extraction remaining Fructus Schisandrae Chinensis medicinal residues and Radix Salviae Miltiorrhizae decoction dregs also containing substantial amounts of cellulose and lignin etc. into
Point, additionally containing nutritional labelings such as a small amount of protein, polysaccharide, due to the quality of Radix Salviae Miltiorrhizae it is hard, it is difficult to be degraded, so as to limit
Its application is made.The present invention by by Radix Salviae Miltiorrhizae decoction dregs carry out it is expanded after be crushed to particle diameter for 4-6mm, destroy few fibers composition
Structure, make fibre composition loosely organized and be easy to the utilization that is degraded；Additionally, by Fructus Schisandrae Chinensis medicinal residues it is size-reduced to particle diameter be 1-
3mm, by the control to corn cob, Radix Salviae Miltiorrhizae decoction dregs and Fructus Schisandrae Chinensis medicinal residues particle diameter, realizes the optimization of culture medium for cultivating structure, changes
The permeability of culture medium for cultivating has been apt to it, the alimentation and growth beneficial to Hypsizygus marmoreuss.
Because the mass percent of corn cob is high, if particle diameter is too big, bottle weight can be caused partially light, charge level when causing mycelium stimulation
Out-of-flatness；If particle diameter is too little, bottle can be caused to lay particular stress on again, culture medium for cultivating structure is excessively fine and close, is unfavorable for the growth of mycelia.
The quality of wheat bran is loose, and the elasticity for culture medium provides effectively combination, improves the breathability of mycelia, adding by wheat bran
Plus, the consumption of Testa oryzae is reduced, the cost of raw material is further reduced.About 900-1000 yuan/ton of the price of wheat bran, and Testa oryzae
About 2500-3000 yuan/ton of price, about 1500-2000 yuan/ton of the price of wheat bran.
Compared with prior art, the invention has the beneficial effects as follows：By adding a small amount of helping in liquid spawn culture medium
Long agent, can be such that strain breeds quickly, and strain coverage rate is high, significantly improves spawn activity and disease-resistant performance, have very to spider web disease
Good resistivity.The cultural method of the Hypsizygus marmoreuss that the present invention is provided is simple, low cost, by liquid spawn culture medium and cultivation
Being harmful to for culture medium, shortens cultivation period, and the Hypsizygus marmoreuss yield that cultivation is obtained is high, quality is good, in good taste, anti-microbial property is good.
Specific embodiment
Below by specific embodiment, the present invention is described in further detail.
The liquid spawn culture medium of the Hypsizygus marmoreuss of embodiment one
The liquid spawn culture medium of Hypsizygus marmoreuss, including following component and its parts by weight：78 parts of glucose, analysis for soybean powder 16
650 parts of part, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, defoamer THIX-2980.06 parts, 4 parts of growth regulator and water.Growth regulator is by day
Numb element and propylene glycol alginate sodium sulfate are with 5: 2 weight than composition.
Preparation method：Glucose, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate and growth regulator are weighed, water is added, froth breaking is added
Agent, stirring and dissolving, sterilizing obtains the liquid spawn culture medium of Hypsizygus marmoreuss.
The liquid spawn culture medium of the Hypsizygus marmoreuss of embodiment two
The liquid spawn culture medium of Hypsizygus marmoreuss, including following component and its parts by weight：80 parts of glucose, analysis for soybean powder 20
Part, 4 parts of potassium dihydrogen phosphate, 3 parts of magnesium sulfate, 0.1 part of defoamer THIX-298,5 parts of growth regulator and 700 parts of water.Growth regulator is by day
Numb element and propylene glycol alginate sodium sulfate are with 3: 1 weight than composition.Preparation method is with embodiment one.
The liquid spawn culture medium of the Hypsizygus marmoreuss of embodiment three
The liquid spawn culture medium of Hypsizygus marmoreuss, including following component and its parts by weight：74 parts of glucose, analysis for soybean powder 15
Part, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.08 part of defoamer THIX-298,3 parts of growth regulator and 600 parts of water.Growth regulator by
Gastrodine and propylene glycol alginate sodium sulfate are with 5: 2 weight than composition.Preparation method is with embodiment one.
The liquid spawn culture medium of the Hypsizygus marmoreuss of comparative example 1
The liquid spawn culture medium of Hypsizygus marmoreuss, including following component and its parts by weight：78 parts of glucose, analysis for soybean powder 16
Part, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.06 part of defoamer THIX-298,4 parts of growth regulator and 650 parts of water.Growth regulator is
Gastrodine.Preparation method is with embodiment one.
The liquid spawn culture medium of the Hypsizygus marmoreuss of comparative example 2
The liquid spawn culture medium of Hypsizygus marmoreuss, including following component and its parts by weight：78 parts of glucose, analysis for soybean powder 16
Part, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.06 part of defoamer THIX-298,4 parts of growth regulator and 650 parts of water.Growth regulator is
Propylene glycol alginate sodium sulfate.Preparation method is with embodiment one.
The liquid spawn culture medium of the Hypsizygus marmoreuss of comparative example 3
The liquid spawn culture medium of Hypsizygus marmoreuss, including following component and its parts by weight：78 parts of glucose, analysis for soybean powder 16
Part, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.06 part of defoamer THIX-298,4 parts of growth regulator and 650 parts of water.Growth regulator by
Gastrodine and propylene glycol alginate sodium sulfate are with 1: 1 weight than composition.Preparation method is with embodiment one.
The cultivation of example IV Hypsizygus marmoreuss
Prepare the liquid spawn culture medium of embodiment one；
The culture of fermentation tank strain：The liquid spawn culture medium of embodiment one is added in fermentation tank, sterilizing, fermentation tank temperature
Degree is down to 22 DEG C, the Hypsizygus marmoreuss strain of bis- grades of kind cultures of Jing is inoculated in fermentation tank and is cultivated, the aerobic culture 6 under the conditions of 22 DEG C
My god, obtain fermentor liquid strain；It is pollution-free after testing, reach the condition for the cultivation of subsequent production bottle；
Prepare culture medium for cultivating：Weigh corn cob, the Fructus Schisandrae Chinensis medicinal residues of 5 parts by weight, 15 weight portions of 45 parts by weight
Several Radix Salviae Miltiorrhizae decoction dregs and the Calx of 0.5 parts by weight, the temperature that total consumption 2/3 is added while stirring is 10 DEG C of water, stirring
60min, is subsequently adding Testa oryzae, the soybean cover of 7 parts by weight, the wheat bran of 16 parts by weight and 1.5 weight portions of 10 parts by weight
Several conch meals, adds remaining water, wet-mixing 30min to obtain culture medium in wet-mixing, bottling, sterilizing；The water content of culture medium
It is 6.2 for 64%, pH.The particle diameter of the corn cob is 2-8mm；The Radix Salviae Miltiorrhizae decoction dregs are crushed to particle diameter Jing after bulking machine is expanded
4-6mm；The Fructus Schisandrae Chinensis medicinal residues it is size-reduced to particle diameter be 1-3mm；
Inoculation Hypsizygus marmoreuss strain：It is inoculated with using liquid inoculator, using the culture bottle (making a call to three holes) of 850ml, inoculum concentration is：
20mL/ bottles, inoculation temperature is 20 DEG C；
Mycelia culture：Temperature is 20 DEG C, and humidity is 68%, and gas concentration lwevel is 2000～3000ppm, cultivates 24 days bacterium
The full bottle of filament length, then through the after-ripening of 29 days, mycelia switched to khaki；
Mycelium stimulation：Mycelia is covered with and after after-ripening, using steamed bread type mycelium stimulation knife mycelium stimulation fruiting, strain central authorities is gently pressed with recessed ware
Under, while surrounding is scratched；
Fruiting is simultaneously harvested, and obtains Hypsizygus marmoreuss.The yield of per bottle of production bottle is 193.6g, and cap is not deployed, a diameter of
1.5cm, lamella, stem white, mushroom shape is neat, and stem is sturdy, tall and straight, is highly 7.3cm, and free from admixture goes mouldy.To in Hypsizygus marmoreuss
The content of arsenic, lead, hydrargyrum etc. detected, meet GB 7096-2014's (national food safety standard edible fungi and its product)
Regulation, safety is good.
The cultivation of the Hypsizygus marmoreuss of comparative example 4
With differing only in for example IV：In the step of preparing culture medium for cultivating, the Fructus Schisandrae Chinensis of 10 parts by weight are weighed
The Radix Salviae Miltiorrhizae decoction dregs of medicinal residues, 10 parts by weight.That is, the weight ratio of Fructus Schisandrae Chinensis medicinal residues and Radix Salviae Miltiorrhizae decoction dregs becomes 1: 1 by 1: 5.
Experiment in cultivation example
Example one, embodiment two, comparative example 1, comparative example 2 and liquid spawn culture medium difference obtained in comparative example 3
It is inoculated with and is cultivated by the same terms in example IV, as corresponding test group, and using comparative example 4 as cultivation culture
Four groups of the comparative example of base, is compared, as a result as shown in table 1 to the culture effect of Hypsizygus marmoreuss.
The cultivation effect of the different condition of culture of table 1
As known from Table 1, the present invention is provided liquid spawn culture medium and culture medium for cultivating are beneficial to the fast-growth of Hypsizygus marmoreuss,
Under the same terms, it is 6 days that one group of embodiment reaches the fermented bacterium cultivation cycle of production bottle cultivation condition, hence it is evident that than comparative example 1
3 groups of group, 2 groups of comparative example and comparative example are shorter, and the cultivation period of the full bottle of production bottle is also significantly foreshortened to 25 days, and improve product
Amount, product it is in good taste.The cultivation effect that one group of embodiment is optimal, therefore, embodiment one is highly preferred embodiment of the present invention.
Additionally, spider web disease incidence rate of the present invention also to different tests group under the same conditions is counted, as a result such as
Shown in table 2.Spider web disease incidence rate=have sporophore sum/whole sporophore of scab total.As known from Table 2, the present invention is implemented
Liquid spawn culture medium obtained in example one, for the culture of Hypsizygus marmoreuss strain, can significantly improve the resistivity to spider web disease, send out
Sick rate is significantly reduced.
The Hypsizygus marmoreuss sporophore spider web disease incidence rate of the different cultivation conditions of table 2
| Group | Spider web disease incidence rate (%) |
| One group of embodiment | 1.8 |
| Two groups of embodiment | 2.3 |
| 1 group of comparative example | 18.9 |
| 2 groups of comparative example | 21.5 |
| 3 groups of comparative example | 12.6 |
| 4 groups of comparative example | 8.0 |
Above content is to combine specific preferred implementation further description made for the present invention, it is impossible to assert
The present invention be embodied as be confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of without departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (9)
1. a kind of liquid spawn culture medium of Hypsizygus marmoreuss, it is characterised in that：Including following component and its parts by weight：Glucose
74-80 parts, analysis for soybean powder 14-20 part, potassium dihydrogen phosphate 1-4 parts, magnesium sulfate 1-4 parts, defoamer 0.05-0.1 parts, growth regulator 2-6
Part and water 600-700 parts；
The growth regulator is by gastrodine and propylene glycol alginate sodium sulfate with 4-6:The weight of 1-3 is than composition.
2. the liquid spawn culture medium of Hypsizygus marmoreuss according to claim 1, it is characterised in that：Including following component and its weight
Amount number：78 parts of glucose, 16 parts of analysis for soybean powder, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 0.06 part of defoamer, 4 parts of growth regulator and
650 parts of water.
3. the liquid spawn culture medium of Hypsizygus marmoreuss according to claim 2, it is characterised in that：The growth regulator is by gastrodine
With propylene glycol alginate sodium sulfate by weight 5:2 compositions.
4. the liquid spawn culture medium of Hypsizygus marmoreuss according to claim 1, it is characterised in that：The defoamer changes for polyethers
Property silicon oil foam killer.
5. the preparation method of the liquid spawn culture medium of Hypsizygus marmoreuss according to any one of claim 1 to 4, its feature exists
In：Comprise the steps：Glucose, analysis for soybean powder, potassium dihydrogen phosphate, magnesium sulfate and growth regulator are weighed, water is added, froth breaking is added
Agent, stirring and dissolving, sterilizing obtains the liquid spawn culture medium of Hypsizygus marmoreuss.
6. a kind of cultural method of Hypsizygus marmoreuss, it is characterised in that：Comprise the steps：
S1 prepares liquid spawn culture medium：Weigh 74-80 parts, analysis for soybean powder 14-20 part, potassium dihydrogen phosphate 1-4 parts, magnesium sulfate 1-4
Part and growth regulator 2-6 parts, add water 600-700 parts, add defoamer 0.05-0.1 parts, stirring and dissolving to obtain liquid spawn training
Foster base；
The culture of S2 fermentation tank strains：The liquid spawn culture medium is added in fermentation tank, sterilizing, fermentation jar temperature is down to
20-24 DEG C, Hypsizygus marmoreuss strain is inoculated in fermentation tank and is cultivated, aerobic culture 5-8 days under the conditions of 20-24 DEG C obtain fermentation tank
Liquid spawn；
S3 prepares culture medium for cultivating：Weigh corn cob, the Fructus Schisandrae Chinensis medicinal residues of 3-8 parts by weight, the 10-20 of 40-50 parts by weight
The Radix Salviae Miltiorrhizae decoction dregs of parts by weight and the Calx of 0.2-1 parts by weight, the temperature that total consumption 2/3 is added while stirring is 8-15 DEG C
Water, stirs 60-70min, is subsequently adding Testa oryzae, the soybean cover of 5-10 parts by weight, the 12-20 parts by weight of 8-12 parts by weight
Wheat bran and 0.8-2 parts by weight conch meal, add remaining water in wet-mixing, wet-mixing 30-40min obtains cultivation culture
Base, dress production bottle, sterilizing；
S4 is inoculated with the fermentor liquid strain in production bottle；
S5 mycelia culture, mycelia is covered with and after after-ripening, using steamed bread type mycelium stimulation knife mycelium stimulation fruiting；
S6 fruitings are simultaneously harvested, and obtain Hypsizygus marmoreuss；
The growth regulator is by gastrodine and propylene glycol alginate sodium sulfate with 4-6:The weight of 1-3 is than composition.
7. the cultural method of Hypsizygus marmoreuss according to claim 6, it is characterised in that：The water content of the culture medium for cultivating is
63-65%, pH are 6.0-6.6.
8. the cultural method of Hypsizygus marmoreuss according to claim 6, it is characterised in that：The particle diameter of the corn cob is 2-8mm；
The Radix Salviae Miltiorrhizae decoction dregs are crushed to particle diameter Jing after bulking machine is expanded for 4-6mm；The Fructus Schisandrae Chinensis medicinal residues it is size-reduced to particle diameter be 1-
3mm。
9. the cultural method of Hypsizygus marmoreuss according to claim 6, it is characterised in that：The temperature of the mycelia culture is 20-
22 DEG C, humidity is 65-75%, and gas concentration lwevel is 1000-3500ppm.
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| CN111527992A (en) * | 2020-05-06 | 2020-08-14 | 江苏华绿生物科技股份有限公司 | Culture medium for hypsizigus marmoreus |
| CN113383677A (en) * | 2021-07-21 | 2021-09-14 | 西充星河生物科技有限公司 | Hypsizigus marmoreus tissue isolation medium and preparation method thereof |
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