CN104496688B - The application of ganoderma lucidum solid state cultivation culture medium and MAILUONING ZHUSHEYE production discarded object in cultivation of glossy ganoderma - Google Patents
The application of ganoderma lucidum solid state cultivation culture medium and MAILUONING ZHUSHEYE production discarded object in cultivation of glossy ganoderma Download PDFInfo
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- CN104496688B CN104496688B CN201410831244.4A CN201410831244A CN104496688B CN 104496688 B CN104496688 B CN 104496688B CN 201410831244 A CN201410831244 A CN 201410831244A CN 104496688 B CN104496688 B CN 104496688B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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Abstract
The present invention provides the application of ganoderma lucidum solid state cultivation culture medium and MAILUONING ZHUSHEYE production discarded object in cultivation of glossy ganoderma, belongs to natural resources of Chinese medicinal materials technical field.The culture medium contains：30 60 parts of cotton seed hulls, 10 20 parts of wheat bran, 0.5 2 parts of lime, 0.5 2 parts of land plaster, MAILUONING ZHUSHEYE 15 50 parts of discarded object of production；The MAILUONING ZHUSHEYE production discarded object is that the dregs of a decoction after the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle are boiled in decocting.The present invention also provides application of the MAILUONING ZHUSHEYE production discarded object in ganoderma lucidum solid state cultivation.Culture medium of the present invention substitutes cotton seed hulls using MAILUONING ZHUSHEYE production discarded object, it can solve the problem that MAILUONING ZHUSHEYE production discarded object develops the serious technical problem of difficult, environmental pollution, while cultivation of glossy ganoderma cost is reduced, the service efficiency of natural resources of Chinese medicinal materials is significantly improved.
Description
Technical field
The invention belongs to natural resources of Chinese medicinal materials technical field, and in particular to ganoderma lucidum solid state cultivation culture medium and MAILUONING ZHUSHEYE life
Produce application of the discarded object in cultivation of glossy ganoderma.
Background technology
The root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle are boiled using decocting by Nanjing pharmaceutcal corporation, Ltd, then using ethanol precipitation, second
The method such as acetoacetic ester is refined is removed in decoction liquor obtains MAILUONING ZHUSHEYE after impurity.In the production process of MAILUONING ZHUSHEYE
The discarded object of generation includes the dregs of a decoction after decocting is boiled, the precipitation in the decoction liquor and ethanol precipitation methods that are remained in the dregs of a decoction.
Nanjing pharmaceutcal corporation, Ltd produces a large amount of above-mentioned discarded objects per year, and these discarded objects are stacked at FX, easily rotten
Lose, the precipitation (alcohol hypostasis) in severe contamination, especially ethanol precipitation methods is caused to environment, viscosity is high, it is difficult to handle.This
A little discarded objects cause great burden to Nanjing pharmaceutcal corporation, Ltd, not only exhaust a large amount of manpower and materials, and cause money
The waste and serious environmental pollution in source.
Ganoderma lucidum is commonly called as polyporus lucidus, is a kind of precious medicinal fungi, with very high medical value.With mushroom industry
Development, culturing raw material day is becoming tight, and reservoir area of Three Gorges " conceding the land to forestry " makes the agricultural by-products such as cotton seed hulls, stalk drastically reduce,
Price is risen steadily, and cultivation of glossy ganoderma cost is increasingly increased.
The content of the invention
It is an object of the invention to provide ganoderma lucidum solid state cultivation culture medium, the culture medium is discarded using MAILUONING ZHUSHEYE production
Thing substitutes cotton seed hulls, can not only solve a large amount of MAILUONING ZHUSHEYEs production discarded objects and develop difficult, environmental pollution is serious
Technical problem, and while cultivation of glossy ganoderma cost is reduced, significantly improve the service efficiency of natural resources of Chinese medicinal materials.
It is a further object of the present invention to provide application of the MAILUONING ZHUSHEYE production discarded object in ganoderma lucidum solid state cultivation.
The purpose of the present invention adopts the following technical scheme that realization.
A kind of ganoderma lucidum solid state cultivation culture medium, the composition containing following mass parts：30-60 parts of cotton seed hulls, wheat bran 10-20
Part, 0.5-2 parts of lime, 0.5-2 parts of land plaster, MAILUONING ZHUSHEYE 15-50 parts of discarded object of production；The MAILUONING ZHUSHEYE life
Production method is included boiling the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle using decocting and removed using ethanol precipitation methods miscellaneous in decoction liquor
The step of matter；The MAILUONING ZHUSHEYE production discarded object is that the medicine produced after the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle is boiled in decocting
Slag.In MAILUONING ZHUSHEYE production, the mass parts of the root of bidentate achyranthes 1~4, the mass parts of radix scrophulariae 1~4, the mass parts of the stem of noble dendrobium 1~4 and honeysuckle 1
~4 mass parts.
It is preferred that technical scheme in, MAILUONING ZHUSHEYE production discarded object be will dregs of a decoction drying, crush after
Arrive.
It is preferred that technical scheme in, MAILUONING ZHUSHEYE production discarded object also includes the decoction remained in the dregs of a decoction
Precipitation in liquid and ethanol precipitation methods.Precipitation (ethanol pellet) in ethanol precipitation methods can be significantly improved in fructification
The content of GL-B, enhances the medical value of ganoderma lucidum.
It is preferred that technical scheme in, ganoderma lucidum solid state cultivation culture medium contains the composition of following mass parts：Cotton seed hulls 30-60
Part, 10-20 parts of wheat bran, 0.5-2 parts of lime, 0.5-2 parts of land plaster, MAILUONING ZHUSHEYE 15-30 parts of discarded object of production.
It is preferred that technical scheme in, MAILUONING ZHUSHEYE production discarded object will be remained in the dregs of a decoction, the dregs of a decoction
Decoction liquor, ethanol pellet mixing, drying are obtained after crushing；The ratio of the decoction liquor remained in the dregs of a decoction, the dregs of a decoction is 0.5-
2g：0.5-1.5mL：0.8-1.5g.
It is preferred that technical scheme in, in the culture medium also include equivalent to cotton seed hulls, wheat bran, lime, land plaster and arteries and veins
Network injection for curing production discarded object gross mass 150-186% water.
The present invention also provides application of the MAILUONING ZHUSHEYE production discarded object in ganoderma lucidum solid state cultivation, the Mailuoning note
Penetrating liquid production method includes being boiled the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle using decocting and being removed using ethanol precipitation methods decocting
In liquid the step of impurity；The discarded object is that the dregs of a decoction after the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle are boiled in decocting.
It is preferred that technical scheme in, the discarded object also includes the decoction liquor that remains and ethanol precipitation methods in the dregs of a decoction
In precipitation.
It is preferred that technical scheme in, it is useless added with MAILUONING ZHUSHEYE production in the ganoderma lucidum solid state cultivation culture medium
Gurry.
It is preferred that technical scheme in, the ganoderma lucidum solid state cultivation culture medium contains the composition of following mass parts：Cotton seed hulls 30-
60 parts, 10-20 parts of wheat bran, 0.5-2 parts of lime, 0.5-2 parts of land plaster, MAILUONING ZHUSHEYE production 15-50 parts of discarded object.
Using culture medium solid state cultivation ganoderma lucidum of the present invention, compared with prior art, advantage is：
1) cultivation effect is good：The method for producing discarded object cultivating ganoderma using MAILUONING ZHUSHEYE is feasible, and its yield is high, son
Entity quality is good, and ganoderma polyoses content is high.It is many that the ethanol pellet added in culture medium can significantly improve ganoderma lucidum in fructification
The content of sugar, enhances the medical value of ganoderma lucidum, wide market.
2) it is economical：MAILUONING ZHUSHEYE discarded object is taken full advantage of, the expense of the processing parenteral solution discarded object is not only saved,
And the raw material used in the cultivation of glossy ganoderma of parts of traditional are instead of, cultivation of glossy ganoderma cost is significantly reduced, has been reached higher
Comprehensive economic results in society.
3) it is environmentally friendly：On the one hand, because parenteral solution discarded object instead of the raw material used in the cultivation of glossy ganoderma of parts of traditional, fill
Divide and utilize natural resources of Chinese medicinal materials.On the other hand, the original pollution to stack, landfill, burning disposal discarded object are brought in pharmaceutical factory is solved.
Brief description of the drawings
Fig. 1 shows the cultivation result of ganoderma lucidum.
Embodiment
The specific method that MAILUONING ZHUSHEYE produces discarded object cultivating ganoderma bacterium is described in detail below.
The MAILUONING ZHUSHEYE of embodiment 1 produces discarded object
The production method of MAILUONING ZHUSHEYE：By the mass parts of the root of bidentate achyranthes 1, the mass parts of radix scrophulariae 1, the mass parts of the stem of noble dendrobium 1 and honeysuckle 1
Mass parts are mixed, 4~8 times of amount water of dosing material gross mass, after soaking 0.5~3 hour, are decocted and are extracted 1~3 time, 1~3 is small every time
When, filtering removes the dregs of a decoction, merges decoction liquor, and relative density when being concentrated into 65 DEG C is 1.20~1.40；Decoction after concentration
Ethanol is added in liquid, concentration of alcohol in system is reached 75~80% (volumn concentrations), stands 8~48 hours, cross and filter off
Except precipitation (ethanol pellet), take supernatant, reclaim and eliminate ethanol, relative density when being concentrated into 65 DEG C for 1.05~
1.15；Extracted with 2~4 by amount ethyl acetate, MAILUONING ZHUSHEYE is made after the recovered solvent of extract.
The discarded object produced in the production process of MAILUONING ZHUSHEYE boils the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and gold and silver including decocting
The decoction liquor (being abbreviated as waste liquid) and ethanol pellet remained in the dregs of a decoction (being abbreviated as the dregs of a decoction) that are produced after spending, the dregs of a decoction.
Nutrient composition content in the detection dregs of a decoction, waste liquid and ethanol pellet.Content of starch uses enzymatic isolation method, crude protein content
Using Kjeldahl's method, total sugar content uses phend-sulphuric acid, and crude fiber content is disappeared cooking method using soda acid.Measurement result such as table
Shown in 1-1.
Nutrient composition content in the table 1-1 dregs of a decoction, waste liquid and ethanol pellet
From table 1-1 experimental data can be seen that waste liquid and ethanol pellet in starch, crude protein, total sugar content enrich,
Crude fiber content in the dregs of a decoction enriches, and these are all the indispensable nutriments of edible fungi growth.
Nutritional ingredient is fewer if the dregs of a decoction are used merely, it is contemplated that adding waste liquid and ethanol pellet mixing, therefore
Two big group experiments are carried out, the respectively discarded object A in embodiment 2 and discarded object B1-B4 are added in basic composition.And waste liquid
It is high with total sugar content in ethanol pellet, and the main component of ganoderma lucidum is GL-B, expects to add waste liquid and ethanol precipitation
Thing can improve the content of polysaccharide in ganoderma lucidum.
The MAILUONING ZHUSHEYE of embodiment 2 produces the preparation of discarded object
1. being crushed to 10 mesh sizes after the dregs of a decoction are dried into 3h in 105 DEG C, discarded object A is obtained.Discarded object A is adopted in the present embodiment
Prepared with the method.
2. according to discarded object A2g, ethanol pellet 1g, waste liquid 1mL ratios, by three kinds of material mixings, 3h is dried in 105 DEG C
After be crushed to 10 mesh sizes, obtain discarded object B1.
3. according to discarded object A0.5g, ethanol pellet 0.8g, waste liquid 1.4mL ratios, by above-mentioned three kinds of material mixings, in
10 mesh sizes are crushed to after 105 DEG C of drying 3h, discarded object B2 is obtained.
4. according to discarded object A1g, ethanol pellet 1g, waste liquid 1mL ratios, by three kinds of material mixings, 3h is dried in 105 DEG C
After be crushed to 10 mesh sizes, obtain discarded object B3.
5. according to discarded object A1.2g, ethanol pellet 1.2g, waste liquid 0.7mL ratios, by three kinds of material mixings, in 105 DEG C
10 mesh sizes are crushed to after drying 3h, discarded object B4 is obtained.
The MAILUONING ZHUSHEYE of embodiment 3 produces application of the discarded object in solid state cultivation ganoderma lucidum
1. prepare culture medium
Culture medium raw material is sampled by table 3-1, mixing turns, fully mixed thoroughly, obtains solid mixture repeatedly.In solid
The 160-170% water equivalent to raw material gross mass is added in mixture, is stirred, culture medium 1~7 is obtained.
Added with the water equivalent to solid mixture quality 160-170% in culture medium 1~7, now hand picks up a training
Base is supported, water has gently been pinched and has oozed out without lower drop.
The raw material and its proportioning of table 3-1 culture mediums
2. solid state cultivation ganoderma lucidum
The culture medium 1-7 prepared is respectively charged into cultivating bag (fire resistant polypropylene polybag), with external diameter about 2.5cm
Cylindrical wooden club bottom is inserted into the middle of the cultivating bag fed to reserve the space that can be inoculated with, sack puts ring, button
After upper supporting lid, cultivating bag and loop-carrier are placed in 121.3 DEG C of high-pressure sterilizing pot and sterilized 1.5 hours.Sterilizing terminates, and waits to cultivate
When bag temperature is cooled to less than 30 DEG C, on the superclean bench of desinfection chamber, lucidum strain is inoculated with.Cultivating bag after inoculation is in 25
DEG C or so, shading culture under relative air humidity 60-70% environment, pollution is rejected during culture.With the whiteness of mycelial growth,
Dense degree judges whether pollution, and whiteness is higher, dense degree is higher shows that mycelial growth is better.
The mycelia cultivating bag for selecting culture medium 1~6 moves into mushroom house, takes off lid, pulls down old fungus block and to mushroom mouthful mycelium stimulation, keeps mushroom
The temperature in room is sooner or later each daily to divulge information once at 25-28 DEG C, each 30min.To form former base when aerial hyphae is twisted together and stretch out bag
During mouth, by the humid control of mushroom house between 85%-95%, harvested after fructification is ripe.Cultivation of glossy ganoderma result is shown in Fig. 1.
The culture situation of mycelia in each culture mediums of table 3-2-1
Note：" densification ", " richer ", " dense " reflection mycelium growth vigor gradually weaken in table 3-2-1.
The detection case of table 3-2-2 1~6 cultivating ganoderma of culture medium
Note：Biological efficiency=(fructification dry weight/compost dry weight) × 100% in table 3-2-2；The content of GL-B
Assay method is according to 2010 editions《Chinese Pharmacopoeia》Method determine
Compared from table 3-2-1, table 3-2-2 and Fig. 1：Compare the growing state of ganoderma lucidum in each culture medium, using culture
Base 2,3 cultivating ganodermas, biological efficiency, ganoderma polyoses content and culture medium 1 are suitable, due to using Mailuoning in culture medium 2,3
Parenteral solution production discarded object instead of substantial amounts of cotton seed hulls, so cost is greatly lowered, promote the use of can achieve it is very good
Economic benefit, while reducing pollution and processing cost of the discarded object to environment.Using the cultivating ganoderma of culture medium 5, mycelia
Growing way, biological efficiency, ganoderma polyoses content are significantly better than culture medium 1,2 and 3, promote the use of can not only reduce production cost and
Environmental pollution, while extraordinary technique effect can be obtained.
In addition, by repetition test, finding in culture medium 5 using discarded object B1, B2 or B4 replacement B3, the effect of cultivating ganoderma
Fruit is suitable with culture medium 5.
Claims (7)
1. a kind of ganoderma lucidum solid state cultivation culture medium, it is characterised in that the composition containing following mass parts：30-60 parts of cotton seed hulls, bran
10-20 parts of skin, 0.5-2 parts of lime, 0.5-2 parts of land plaster, MAILUONING ZHUSHEYE 15-50 parts of discarded object of production；The train of thought
Injection for curing production method is included boiling the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle using decocting and removed using ethanol precipitation methods
In decoction liquor the step of impurity；The MAILUONING ZHUSHEYE production discarded object boils the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle including decocting
The decoction liquor and ethanol pellet remained in the dregs of a decoction that produce afterwards, the dregs of a decoction.
2. the culture medium of ganoderma lucidum solid state cultivation according to claim 1, it is characterised in that the composition containing following mass parts：Cotton
30-60 parts of seed shell, 10-20 parts of wheat bran, 0.5-2 parts of lime, 0.5-2 parts of land plaster, MAILUONING ZHUSHEYE production discarded object
15-30 parts.
3. the culture medium of ganoderma lucidum solid state cultivation according to claim 2, it is characterised in that the MAILUONING ZHUSHEYE production is useless
Gurry is decoction liquor, the ethanol pellet mixing that will be remained in the dregs of a decoction, the dregs of a decoction, and drying is obtained after crushing；The dregs of a decoction,
Decoction liquor, the ratio of ethanol pellet remained in the dregs of a decoction is 0.5-2g：0.5-1.5mL：0.8-1.5g.
4. according to one of the claim 1-3 ganoderma lucidum solid state cultivation culture mediums, it is characterised in that also include in the culture medium
Equivalent to the water that cotton seed hulls, wheat bran, lime, land plaster and MAILUONING ZHUSHEYE produce discarded object gross mass 150-186%.
5. MAILUONING ZHUSHEYE produces application of the discarded object in ganoderma lucidum solid state cultivation, the MAILUONING ZHUSHEYE production method bag
Include the step of boiling the root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and honeysuckle using decocting and remove impurity in decoction liquor using ethanol precipitation methods；
Precipitation in the discarded object is the dregs of a decoction after decocting is boiled, remained in the dregs of a decoction decoction liquor and ethanol precipitation methods.
6. application of the MAILUONING ZHUSHEYE production discarded object in ganoderma lucidum solid state cultivation according to claim 5, its feature exists
In the ganoderma lucidum solid state cultivation culture medium discarded object is produced added with the MAILUONING ZHUSHEYE.
7. application of the MAILUONING ZHUSHEYE production discarded object in ganoderma lucidum solid state cultivation according to claim 6, its feature exists
Contain the composition of following mass parts in the ganoderma lucidum solid state cultivation culture medium：30-60 parts of cotton seed hulls, 10-20 parts of wheat bran, lime
0.5-2 parts, 0.5-2 parts of land plaster, MAILUONING ZHUSHEYE production 15-50 parts of discarded object.
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| CN105110858B (en) * | 2015-09-21 | 2018-11-16 | 株洲千金药业股份有限公司 | A kind of Medium for Ganoderma lucidum and preparation method thereof |
| CN105237238A (en) * | 2015-11-17 | 2016-01-13 | 孙铭岐 | Culture medium of lucid ganoderma and cultivation method |
| CN113249227B (en) * | 2021-05-11 | 2022-11-08 | 聊城大学 | Novel lucid ganoderma strain and cultivation application thereof based on medicinal residue fungus bag |
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| KR101423983B1 (en) * | 2011-12-28 | 2014-08-01 | 김대석 | Production method of high quality Medium for cultivating a mushroom using them |
| CN102523928A (en) * | 2012-01-16 | 2012-07-04 | 广东省微生物研究所 | Method for culturing lucid ganoderma by using oral liquid dregs |
| CN102838429B (en) * | 2012-09-13 | 2014-08-20 | 江苏省农业科学院 | Vegetable organic culture medium by using Chinese medicine residues as main raw materials and preparation method thereof |
| CN103739351A (en) * | 2013-12-09 | 2014-04-23 | 广西柳城县成霖农业科技有限公司 | Fungus bags used for bag cultivation of lucid ganoderma |
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