CN104496688A - Lucid ganoderma solid cultivation medium and application of Mailuoning injection production waste in lucid ganoderma cultivation - Google Patents
Lucid ganoderma solid cultivation medium and application of Mailuoning injection production waste in lucid ganoderma cultivation Download PDFInfo
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Abstract
The invention provides a lucid ganoderma solid cultivation medium and an application of Mailuoning injection production waste in lucid ganoderma cultivation, and belongs to the technical field of traditional Chinese medicine resources. The cultivation medium comprises 30 to 60 parts of cottonseed hulls, 10-20 parts of wheat bran, 0.5-2 parts of lime, 0.5-2 parts of gypsum powder, and 15-50 parts of Mailuoning injection production waste. The Mailuoning injection production waste is dregs obtained after achyranthes roots, figwort roots, stems of noble dendrobium and honeysuckle flowers are decocted. The invention also provides an application of Mailuoning injection production waste in lucid ganoderma cultivation. The cultivation medium provided by the invention uses the Mailuoning injection production waste to replace the cottonseed hulls, so that the technical problems that the Mailuoning injection production waste is difficult to develop and utilize, and seriously pollutes the environment can be solved, the lucid ganoderma cultivation cost is reduced, and the service efficiency of the traditional Chinese medicine resources is obviously improved.
Description
Technical field
The invention belongs to natural resources of Chinese medicinal materials technical field, be specifically related to glossy ganoderma solid state cultivation substratum and the application of MAILUONING ZHUSHEYE production waste in cultivation of glossy ganoderma.
Background technology
Nanjing pharmaceutcal corporation, Ltd adopts the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle, obtains MAILUONING ZHUSHEYE in the method removing decoction liquor such as then adopt alcohol settling, ethyl acetate refining after impurity.Precipitation in decoction liquor residual in the dregs of a decoction after the waste produced in the production process of MAILUONING ZHUSHEYE comprises soak by water, the dregs of a decoction and ethanol precipitation methods.
Nanjing pharmaceutcal corporation, Ltd produces a large amount of above-mentioned waste per year, and these wastes are stacked at FX, and very easily corrupt, cause severe contamination to environment, the precipitation (alcohol hypostasis) especially in ethanol precipitation methods, viscosity is high, is difficult to process.These wastes cause great burden to Nanjing pharmaceutcal corporation, Ltd, not only exhaust a large amount of manpower and materials, and cause the waste of resource and serious environmental pollution.
Glossy ganoderma is commonly called as Ganoderma lucidum seu Japonicum, is a kind of medicinal fungi of preciousness, has very high pharmaceutical use.Along with the development of mushroom industry, culturing raw material day is becoming tight, and reservoir area of Three Gorges " conceding the land to forestry " makes the agricultural by-products such as cotton seed hulls, stalk sharply reduce, and price rises steadily, and cultivation of glossy ganoderma cost strengthens day by day.
Summary of the invention
The object of this invention is to provide glossy ganoderma solid state cultivation substratum, this substratum adopts MAILUONING ZHUSHEYE to produce waste and substitutes cotton seed hulls, a large amount of MAILUONING ZHUSHEYE can not only be solved and produce waste exploitation difficulty, the technical problem that environmental pollution is serious, and while reduction cultivation of glossy ganoderma cost, significantly improve the service efficiency of natural resources of Chinese medicinal materials.
Another object of the present invention is to provide MAILUONING ZHUSHEYE and produces the application of waste in glossy ganoderma solid state cultivation.
Object of the present invention adopts following technical scheme to realize.
A kind of glossy ganoderma solid state cultivation substratum, the composition containing following mass parts: cotton seed hulls 30-60 part, wheat bran 10-20 part, lime 0.5-2 part, terra alba 0.5-2 part, MAILUONING ZHUSHEYE produce waste 15-50 part; Described MAILUONING ZHUSHEYE production method comprises the step adopting impurity in the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle and employing ethanol precipitation methods removing decoction liquor; It is the dregs of a decoction produced after the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle that described MAILUONING ZHUSHEYE produces waste.In MAILUONING ZHUSHEYE is produced, the root of bidentate achyranthes 1 ~ 4 mass parts, radix scrophulariae 1 ~ 4 mass parts, the stem of noble dendrobium 1 ~ 4 mass parts and Japanese Honeysuckle 1 ~ 4 mass parts.
In preferred technical scheme, it is obtain after described dregs of a decoction oven dry, pulverizing that described MAILUONING ZHUSHEYE produces waste.
In preferred technical scheme, described MAILUONING ZHUSHEYE production waste also comprises the precipitation in decoction liquor residual in the described dregs of a decoction and ethanol precipitation methods.Precipitation (ethanol pellet) in ethanol precipitation methods can significantly improve the content of ganoderan in sporophore, enhances the pharmaceutical use of glossy ganoderma.
In preferred technical scheme, glossy ganoderma solid state cultivation substratum contains the composition of following mass parts: cotton seed hulls 30-60 part, wheat bran 10-20 part, lime 0.5-2 part, terra alba 0.5-2 part, MAILUONING ZHUSHEYE produce waste 15-30 part.
In preferred technical scheme, it is by decoction liquor residual in the described dregs of a decoction, the dregs of a decoction, ethanol pellet mixing that described MAILUONING ZHUSHEYE produces waste, dries, obtains after pulverizing; The ratio of decoction liquor residual in the described dregs of a decoction, the dregs of a decoction is 0.5-2g:0.5-1.5mL:0.8-1.5g.
In preferred technical scheme, also comprise in described substratum and be equivalent to the water that cotton seed hulls, wheat bran, lime, terra alba and MAILUONING ZHUSHEYE produce waste total mass 150-186%.
The present invention also provides MAILUONING ZHUSHEYE to produce the application of waste in glossy ganoderma solid state cultivation, and described MAILUONING ZHUSHEYE production method comprises the step adopting impurity in the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle and employing ethanol precipitation methods removing decoction liquor; Described waste is the dregs of a decoction after the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle.
In preferred technical scheme, described waste also comprises the precipitation in decoction liquor residual in the described dregs of a decoction and ethanol precipitation methods.
In preferred technical scheme, be added with described MAILUONING ZHUSHEYE in described glossy ganoderma solid state cultivation substratum and produce waste.
In preferred technical scheme, described glossy ganoderma solid state cultivation substratum contains the composition of following mass parts: cotton seed hulls 30-60 part, wheat bran 10-20 part, lime 0.5-2 part, terra alba 0.5-2 part, MAILUONING ZHUSHEYE produce waste 15-50 part.
Adopt substratum solid state cultivation glossy ganoderma of the present invention, compared with prior art, advantage is:
1) cultivation effect is good: utilize the method for MAILUONING ZHUSHEYE production waste cultivating ganoderma feasible, its output is high, and sporophore quality is good, and ganoderma polyoses content is high.The ethanol pellet added in substratum can significantly improve the content of ganoderan in sporophore, enhances the pharmaceutical use of glossy ganoderma, wide market.
2) economical: to take full advantage of MAILUONING ZHUSHEYE waste, not only save the expense of this waste of process injection liquid, and the starting material that the cultivation of glossy ganoderma that instead of parts of traditional is used, significantly reduce cultivation of glossy ganoderma cost, reach higher comprehensive economic results in society.
3) environmental protection: on the one hand, because injection liquid waste instead of the cultivation of glossy ganoderma of parts of traditional starting material used, makes full use of natural resources of Chinese medicinal materials.On the other hand, pharmaceutical factory is solved originally with the pollution that stacking, landfill, burning disposal waste bring.
Embodiment
Below the concrete grammar of MAILUONING ZHUSHEYE production waste cultivating ganoderma bacterium is described in detail.
Embodiment 1 MAILUONING ZHUSHEYE produces waste
The production method of MAILUONING ZHUSHEYE: the root of bidentate achyranthes 1 mass parts, radix scrophulariae 1 mass parts, the stem of noble dendrobium 1 mass parts and Japanese Honeysuckle 1 mass parts are mixed, add medicinal material total mass 4 ~ 8 times of water gagings, soak after 0.5 ~ 3 hour, decoct extraction 1 ~ 3 time, each 1 ~ 3 hour, filter and remove the dregs of a decoction, merge decoction liquor, relative density when being concentrated into 65 DEG C is 1.20 ~ 1.40; Ethanol is added in decoction liquor after concentration, alcohol concn in system is made to reach 75 ~ 80% (volumn concentrations), leave standstill 8 ~ 48 hours, filter and remove precipitation (ethanol pellet), get supernatant liquor, reclaim and eliminate ethanol, relative density when being concentrated into 65 DEG C is 1.05 ~ 1.15; With 2 ~ 4 by amount extraction into ethyl acetate, extraction liquid is obtained MAILUONING ZHUSHEYE after recycling design.
Decoction liquor (being abbreviated as waste liquid) residual in the dregs of a decoction (being abbreviated as the dregs of a decoction) that the waste produced in the production process of MAILUONING ZHUSHEYE produces after comprising the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle, the dregs of a decoction and ethanol pellet.
Detect the dregs of a decoction, waste liquid and ethanol pellet Middle nutrition component content.Starch content adopts enzymolysis process, and crude protein content adopts Kjeldahl determination, and total sugar content adopts phend-sulphuric acid, and crude fiber content adopts soda acid to disappear cooking method.Measurement result is as shown in table 1-1.
The table 1-1 dregs of a decoction, waste liquid and ethanol pellet Middle nutrition component content
| Nutritive ingredient | Waste liquid (%) | Ethanol pellet (%) | The dregs of a decoction (%) |
| Starch | 7.39 | 4.43 | Content is lower |
| Crude protein | 1.04 | 1.78 | 11.8 |
| Total reducing sugar | 50.04 | 81.20 | Content is lower |
| Robust fibre | Content is lower | Content is lower | 60.78 |
As can be seen from the experimental data of table 1-1, in waste liquid and ethanol pellet, starch, crude protein, total sugar content enrich, and the crude fiber content in the dregs of a decoction enriches, and these are all the nutritive substances of edible fungi growth indispensability.
If use the dregs of a decoction simple, nutritive ingredient is fewer, so consider to add waste liquid and ethanol pellet mixing, organizes test greatly because this has been two, is respectively waste A in embodiment 2 and waste B1-B4 and adds in essentially consist.And total sugar content is high in waste liquid and ethanol pellet, and the main component of glossy ganoderma is ganoderan, expects to add the content that waste liquid and ethanol pellet can improve polysaccharide in glossy ganoderma.
Embodiment 2 MAILUONING ZHUSHEYE produces the preparation of waste
1. be crushed to 10 order sizes after the dregs of a decoction being dried 3h in 105 DEG C, obtain waste A.In the present embodiment, waste A all adopts this method to prepare.
2., according to waste A 2g, ethanol pellet 1g, waste liquid 1mL ratio, by three kinds of material mixing, be crushed to 10 order sizes after drying 3h in 105 DEG C, obtain waste B1.
3., according to waste A 0.5g, ethanol pellet 0.8g, waste liquid 1.4mL ratio, by above-mentioned three kinds of materials mixing, be crushed to 10 order sizes after drying 3h in 105 DEG C, obtain waste B2.
4., according to waste A 1g, ethanol pellet 1g, waste liquid 1mL ratio, by three kinds of material mixing, be crushed to 10 order sizes after drying 3h in 105 DEG C, obtain waste B3.
5., according to waste A 1.2g, ethanol pellet 1.2g, waste liquid 0.7mL ratio, by three kinds of material mixing, be crushed to 10 order sizes after drying 3h in 105 DEG C, obtain waste B4.
Embodiment 3 MAILUONING ZHUSHEYE produces the application of waste in solid state cultivation glossy ganoderma
1. prepare substratum
By culture medium raw material by table 3-1 sampling, mixing, repeatedly turns, fully mixes thoroughly, obtain solid mixture.In solid mixture, add the 160-170% water being equivalent to raw material total mass, stir, obtain substratum 1 ~ 7.
Be added with the water being equivalent to solids mixing amount 160-170% in substratum 1 ~ 7, now hand picks up a substratum, has gently pinched water and has oozed out and not lower.
The raw material of table 3-1 substratum and proportioning thereof
2. solid state cultivation glossy ganoderma
The substratum 1-7 prepared is respectively charged in cultivating bag (fire resistant polypropylene plastics bag), the cylindrical wooden club being about 2.5cm with external diameter is inserted into bottom to reserve the space that can inoculate in the middle of the cultivating bag of having feeded, sack cover pressed on ring, after buckling supporting lid, cultivating bag and loop-carrier are placed in high-pressure sterilizing pot 121.3 DEG C of sterilizings 1.5 hours.Sterilizing terminates, when cultivating bag temperature is cooled to below 30 DEG C, on the Bechtop of sterilisable chamber, and inoculation Ganderma lucidum strain.Postvaccinal cultivating bag in about 25 DEG C, under relative air humidity 60-70% environment shading cultivate, reject between incubation period and pollute.Judge whether to pollute with the whiteness of mycelial growth, dense degree, higher, the dense degree of whiteness is higher shows that mycelial growth is better.
The mycelia cultivating bag selecting substratum 1 ~ 6 moves into mushroom room, takes off lid, and pull down old bacterium block and to mushroom mouth mycelium stimulation, keep the temperature in mushroom room at 25-28 DEG C, morning and evening every day respectively ventilates once, each 30min.When aerial hyphae kink forms former base and when stretching out sack, by the humid control in mushroom room between 85%-95%, gathers after sporophore maturation.Cultivation of glossy ganoderma the results are shown in Figure 1.
The cultivation situation of mycelia in each substratum of table 3-2-1
Attention: in table 3-2-1, " densification ", " denser ", " dense " reflection mycelium growth vigor weaken gradually.
The detection case of table 3-2-2 substratum 1 ~ 6 cultivating ganoderma
| Substratum | Biological efficiency (%) | Ganoderma polyoses content (%) |
| Substratum 1 | 3.42 | 0.438 |
| Substratum 2 | 5.13 | 0.377 |
| Substratum 3 | 3.37 | 0.509 |
| Substratum 4 | 0.93 | 0.803 |
| Substratum 5 | 4.01 | 0.577 |
| Substratum 6 | 1.73 | 0.614 |
Note: biological efficiency=(sporophore dry weight/culture material dry weight) × 100% in table 3-2-2; The content assaying method of ganoderan measures according to the method for 2010 editions " Chinese Pharmacopoeias "
From table 3-2-1, table 3-2-2 and Fig. 1 relatively: the growing state of glossy ganoderma in more each substratum, adopt substratum 2,3 cultivating ganoderma, biological efficiency, ganoderma polyoses content and substratum 1 is suitable, produce waste owing to adopting MAILUONING ZHUSHEYE in substratum 2,3 and instead of a large amount of cotton seed hullss, so cost significantly reduces, promote the use of and can achieve extraordinary economic benefit, decrease waste to the pollution of environment and processing cost simultaneously.Adopt substratum 5 cultivating ganoderma, mycelium growth vigor, biological efficiency, ganoderma polyoses content are significantly better than substratum 1,2 and 3, promote the use of and not only can reduce production cost and environmental pollution, can obtain extraordinary technique effect simultaneously.
In addition, through repetition test, find to adopt waste B1, B2 or B4 to substitute B3 in substratum 5, the effect of cultivating ganoderma is suitable with substratum 5.
Claims (10)
1. a glossy ganoderma solid state cultivation substratum, is characterized in that the composition containing following mass parts: cotton seed hulls 30-60 part, wheat bran 10-20 part, lime 0.5-2 part, terra alba 0.5-2 part, MAILUONING ZHUSHEYE produce waste 15-50 part; Described MAILUONING ZHUSHEYE production method comprises the step adopting impurity in the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle and employing ethanol precipitation methods removing decoction liquor; It is the dregs of a decoction produced after the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle that described MAILUONING ZHUSHEYE produces waste.
2. glossy ganoderma solid state cultivation substratum according to claim 1, it is characterized in that described MAILUONING ZHUSHEYE produce waste be the described dregs of a decoction are dried, pulverize after obtain.
3. glossy ganoderma solid state cultivation substratum according to claim 2, is characterized in that described MAILUONING ZHUSHEYE is produced waste and also comprised precipitation in decoction liquor residual in the described dregs of a decoction and ethanol precipitation methods.
4. the substratum of glossy ganoderma solid state cultivation according to claim 3, is characterized in that the composition containing following mass parts: cotton seed hulls 30-60 part, wheat bran 10-20 part, lime 0.5-2 part, terra alba 0.5-2 part, MAILUONING ZHUSHEYE produce waste 15-30 part.
5. the substratum of glossy ganoderma solid state cultivation according to claim 4, it is characterized in that described MAILUONING ZHUSHEYE produces waste is by decoction liquor residual in the described dregs of a decoction, the dregs of a decoction, ethanol pellet mixing, dries, obtains after pulverizing; Decoction liquor residual in the described dregs of a decoction, the dregs of a decoction, the ratio of ethanol pellet are 0.5-2g:0.5-1.5mL:0.8-1.5g.
6., according to one of claim 1-5 described glossy ganoderma solid state cultivation substratum, it is characterized in that also comprising in described substratum the water being equivalent to cotton seed hulls, wheat bran, lime, terra alba and MAILUONING ZHUSHEYE production waste total mass 150-186%.
7. MAILUONING ZHUSHEYE produces the application of waste in glossy ganoderma solid state cultivation, and described MAILUONING ZHUSHEYE production method comprises the step adopting impurity in the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle and employing ethanol precipitation methods removing decoction liquor; Described waste is the dregs of a decoction after the soak by water root of bidentate achyranthes, radix scrophulariae, the stem of noble dendrobium and Japanese Honeysuckle.
8. MAILUONING ZHUSHEYE produces the application of waste in glossy ganoderma solid state cultivation according to claim 7, it is characterized in that described waste is the precipitation in the decoction liquor and ethanol precipitation methods remained in the dregs of a decoction after soak by water, the described dregs of a decoction.
9. MAILUONING ZHUSHEYE produces the application of waste in glossy ganoderma solid state cultivation according to claim 8, it is characterized in that being added with described MAILUONING ZHUSHEYE in described glossy ganoderma solid state cultivation substratum produces waste.
10. MAILUONING ZHUSHEYE produces the application of waste in glossy ganoderma solid state cultivation according to claim 9, it is characterized in that described glossy ganoderma solid state cultivation substratum contains the composition of following mass parts: cotton seed hulls 30-60 part, wheat bran 10-20 part, lime 0.5-2 part, terra alba 0.5-2 part, MAILUONING ZHUSHEYE produce waste 15-50 part.
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