CN105110858B - A kind of Medium for Ganoderma lucidum and preparation method thereof - Google Patents
A kind of Medium for Ganoderma lucidum and preparation method thereof Download PDFInfo
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- CN105110858B CN105110858B CN201510603655.2A CN201510603655A CN105110858B CN 105110858 B CN105110858 B CN 105110858B CN 201510603655 A CN201510603655 A CN 201510603655A CN 105110858 B CN105110858 B CN 105110858B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
Abstract
The invention discloses a kind of Medium for Ganoderma lucidum, including 50 parts ~ 100 parts of the FUKE QIANJIN PIAN dregs of a decoction；15 parts ~ 30 parts of wheat bran；1 part ~ 5 parts of potassium dihydrogen phosphate；0.1 part ~ 0.3 part of zinc sulfate；Vitamin B10.1 part ~ 0.3 part；1 part ~ 3 parts of gypsum；0.1 part ~ 1 part of triacontanol；1 part ~ 5 parts of glycerol.The invention also discloses the preparation methods of the culture medium simultaneously.The present invention is added the nutritional ingredient of other ganoderma lucidum cultures, can be significantly improved the content of polysaccharide in mature ganoderma lucidum fruitbody, enhance the effect of ganoderma lucidum, improve economic value by the utilization to the FUKE QIANJIN PIAN dregs of a decoction.
Description
Technical field
The present invention relates to fungi culture medium preparation technical fields, and in particular to arrives a kind of Medium for Ganoderma lucidum and the culture medium
Preparation method.
Background technique
Ganoderma lucidum Ganodermataceae Dook is the medicinal rare kind in edible health fungi, is given since ancient times
For mesona,《Sheng Nong's herbal classic》Ganoderma lucidum is classified as top grade, long term usage is made light of one's life by commiting suicide not old, the effect of promoting longevity.Modern medicine confirmation,
Body's immunity can be improved by taking ganoderma lucidum, achieve the effect that prevent and cure diseases.To meet people to the wilderness demand of ganoderma lucidum, modern times
The largely culture ganoderma lucidum by the way of artificial.The compost of prior art culture ganoderma lucidum mainly has Duan Mu, sawdust, cotton seed hulls to be equipped with
Wheat bran, soybean powder, land plaster etc..
According to《Chinese Pharmacopoeia 2010 editions》It is recorded in first, the raw material of FUKE QIANJIN PIAN includes Herba Andrographitis, Radix Angelicae Sinensis, party
Ginseng, philippine flemingia root, cherokee rose root, leatherleaf mahonia, Caulis Spatholobi and single side needle.Other enhancings can also be increased on the basis of this 8 taste medicinal material
The Chinese medicine of effect.The preparation method of FUKE QIANJIN PIAN is：Herba Andrographitis, Radix Codonopsis, Radix Angelicae Sinensis are ground into fine powder, sieving, remaining philippine flemingia root
The equal five tastes add water to cook secondary, 2.5 hours first times, and second 2 hours, collecting decoction filtered, and filtrate is concentrated into relative density
For the clear cream of 1.08~1.12 (85 DEG C), above-mentioned fine powder and right amount of auxiliary materials is added, mixes, particle is made, is pressed into 1000, packet
Sugar-coat or film-coating to get.It can also be application number CN98112696.0, entitled using the Chinese patent literature that has disclosed
A kind of prescription that treating FUKE QIANJIN PIAN disclosed in the process for preparing medicine of gynecological disease and preparation method.
The raw material of FUKE QIANJIN PIAN is generally required including Herba Andrographitis, Radix Angelicae Sinensis, Radix Codonopsis, philippine flemingia root, cherokee rose root, leatherleaf mahonia, chicken
Blood rattan and single side needle.It is remaining in addition to Herba Andrographitis, Radix Angelicae Sinensis, Radix Codonopsis utilization are more abundant in the manufacturing process of FUKE QIANJIN PIAN
Philippine flemingia root, cherokee rose root, leatherleaf mahonia, Caulis Spatholobi four traditional Chinese medicine material will all generate a large amount of dregs of a decoction.Philippine flemingia root has expelling wind and removing dampness, dispersing blood stasis
Detoxify, control the effect of rheumatic arthralgia.The root and stem of Caulis Spatholobi, which are that Yunnan Caulis Spatholobi is medicinal, has promoting circulation of blood and enriching blood, the function of clearing and activating the channels and collaterals
Effect.Leatherleaf mahonia has effects that heat-clearing, eliminating dampness, removing toxic substances.The root of single side needle has promoting blood circulation to remove blood stasis, the effect of reunion of fractured tendons and bones.
Containing the effective component for largely failing to extract completely in these dregs of a decoction, therefore the re-using of the dregs of a decoction will be saved big
The production cost of amount, turns waste into wealth simultaneously, and it is that enterprise brings new profit that new health product, which is made, in the dregs of a decoction.
Ganoderma lucidum polysaccharide is the secondary metabolite of Polyporaceae Ganoderma fungal mycelium, is present in the bacterium of Ganoderma fungi
In filament and fructification.Ganoderma lucidum polysaccharide is one of most effective ingredient of ganoderma lucidum.Ganoderma lucidum polysaccharide has various pharmacological activity；It can mention
High immunity of organisms accelerates blood microcirculation, improves blood oxygen supply capacity, reduces the ineffective oxygen consumption under body stationary state,
Interior free yl is eliminated, the closed stratum of body cell membrane is improved, anti-radiation improves liver, marrow, blood synthetic DNA, RNA, egg
The ability of white matter extends service life etc..A variety of pharmacological activity of ganoderma lucidum are mostly related with ganoderma lucidum polysaccharide.
Therefore, using the FUKE QIANJIN PIAN dregs of a decoction as Medium for Ganoderma lucidum, it is to improve the comprehensive utilization of the FUKE QIANJIN PIAN dregs of a decoction, reduces
The effective ways of waste.
Summary of the invention
The object of the present invention is to provide a kind of Medium for Ganoderma lucidum, and ganoderma lucidum in ganoderma lucidum fruitbody can be improved using the culture medium
The content of polysaccharide.
In order to achieve the above object, providing a kind of Medium for Ganoderma lucidum in one embodiment of the present of invention, including following weight group
Point：
50 parts~100 parts of the FUKE QIANJIN PIAN dregs of a decoction；
15 parts~30 parts of wheat bran；1 part~5 parts of potassium dihydrogen phosphate；0.1 part~0.3 part of zinc sulfate；
Vitamin B10.1 part~0.3 part；1 part~3 parts of gypsum；0.1 part~1 part of triacontanol；
1 part~5 parts of glycerol.
In another embodiment of the invention, disclosed Medium for Ganoderma lucidum includes following components by weight percent：
60 parts~80 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts~30 parts of wheat bran；3 parts~5 parts of potassium dihydrogen phosphate；0.2 part~0.3 part of zinc sulfate；
Vitamin B10.1 part~0.25 part；2 parts~3 parts of gypsum；0.5 part~1 part of triacontanol；
3 parts~5 parts of glycerol.
In another optimal enforcement example of the invention, Medium for Ganoderma lucidum includes following components by weight percent：
70 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts of wheat bran；4 parts of potassium dihydrogen phosphate；0.3 part of zinc sulfate；
Vitamin B10.2 part；2 parts of gypsum；0.6 part of triacontanol；
3 parts of glycerol.
The FUKE QIANJIN PIAN dregs of a decoction are the dregs of a decoction after FUKE QIANJIN PIAN raw material effective component extracting.The dregs of a decoction of the invention can be
Effective component in FUKE QIANJIN PIAN raw material is extracted and the remaining dregs of a decoction, having in the dregs of a decoction using extracting method in the prior art
Effect ingredient has different degrees of reduction, but also containing the various effective components before extracting in raw material, Medium for Ganoderma lucidum is to these
The content requirement of ingredient is not high.
In order to implement above scheme, the present invention discloses the methods for preparing Medium for Ganoderma lucidum, include the following steps：
(1), it is crushed after drying the FUKE QIANJIN PIAN dregs of a decoction, wheat bran is added and stirs evenly；Then potassium dihydrogen phosphate, sulphur is added
Sour zinc, vitamin B1, gypsum, triacontanol and glycerol be uniformly mixed, obtain culture medium siccative；
(2), water is added in culture medium siccative to sterilize after mixing, is reloaded into bacterium bag, obtains Medium for Ganoderma lucidum.
As the embodiment in implementation process of the present invention, the partial size that preferably the FUKE QIANJIN PIAN dregs of a decoction crush is 4mm~10mm；
The water content of Medium for Ganoderma lucidum is 70%~80%；The pH value of Medium for Ganoderma lucidum is 6~6.5.
In conclusion the present invention has the following advantages that：
The nutritional ingredient of other ganoderma lucidum cultures is added by the utilization to the FUKE QIANJIN PIAN dregs of a decoction in the present invention, can be significant
The content for improving polysaccharide in mature ganoderma lucidum fruitbody enhances the medicinal effectiveness of ganoderma lucidum, improves economic value.
Specific embodiment
Embodiment 1
The formula of Medium for Ganoderma lucidum
50 parts of the FUKE QIANJIN PIAN dregs of a decoction；
20 parts of wheat bran；2 parts of potassium dihydrogen phosphate；0.15 part of zinc sulfate；
Vitamin B10.12 part；1 part of gypsum；0.3 part of triacontanol；
2 parts of glycerol.
The FUKE QIANJIN PIAN dregs of a decoction are the dregs of a decoction after FUKE QIANJIN PIAN raw material effective component extracting.
Embodiment 2
60 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts of wheat bran；2.5 parts of potassium dihydrogen phosphate；0.15 part of zinc sulfate；
Vitamin B10.10 part；0.88 part of gypsum；0.35 part of triacontanol；
2.5 parts of glycerol.
The FUKE QIANJIN PIAN dregs of a decoction are the dregs of a decoction after FUKE QIANJIN PIAN raw material effective component extracting.
Embodiment 3
The formula of Medium for Ganoderma lucidum
70 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts of wheat bran；4 parts of potassium dihydrogen phosphate；0.3 part of zinc sulfate；
Vitamin B10.2 part；2 parts of gypsum；0.6 part of triacontanol；
3 parts of glycerol.
Embodiment 4
80 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts of wheat bran；4 parts of potassium dihydrogen phosphate；0.3 part of zinc sulfate；
Vitamin B10.2 part；2 parts of gypsum；0.6 part of triacontanol；
3 parts of glycerol.
The FUKE QIANJIN PIAN dregs of a decoction are the dregs of a decoction after FUKE QIANJIN PIAN raw material effective component extracting.
Embodiment 5
100 parts of the FUKE QIANJIN PIAN dregs of a decoction；
35 parts of wheat bran；3.5 parts of potassium dihydrogen phosphate；0.4 part of zinc sulfate；
Vitamin B10.3 part；3 parts of gypsum；0.6 part of triacontanol；
3 parts of glycerol.
The FUKE QIANJIN PIAN dregs of a decoction are the dregs of a decoction after FUKE QIANJIN PIAN raw material effective component extracting.
The Medium for Ganoderma lucidum preparation method of 1~embodiment of embodiment 5 is：
(1), it is crushed after drying the FUKE QIANJIN PIAN dregs of a decoction, powder particle diameter 5mm；Wheat bran is added to stir evenly；Then plus
Enter potassium dihydrogen phosphate, zinc sulfate, vitamin B1, gypsum, triacontanol and glycerol be uniformly mixed, obtain culture medium siccative；
(2), water is added in culture medium siccative to be uniformly mixed, keeping water content is 70%, and adjusting pH value is 6.2, sterilizing
It is fitted into bacterium bag afterwards, obtains Medium for Ganoderma lucidum.
Comparative examples 1：
The formula of Medium for Ganoderma lucidum
70 parts of the FUKE QIANJIN PIAN dregs of a decoction.
Preparation method is：(1), it is crushed after drying the FUKE QIANJIN PIAN dregs of a decoction, powder particle diameter 5mm；It is equal that water mixing is added
Even, keeping water content is 70%, and adjusting pH value is 6.2, is fitted into bacterium bag after sterilizing, obtains Medium for Ganoderma lucidum.
Comparative examples 2
The formula of Medium for Ganoderma lucidum
25 parts of wheat bran；4 parts of potassium dihydrogen phosphate；0.3 part of zinc sulfate；
Vitamin B10.2 part；2 parts of gypsum；0.6 part of triacontanol；
3 parts of glycerol.
Preparation method：
(1), potassium dihydrogen phosphate, zinc sulfate, vitamin B are added in wheat bran1, gypsum, triacontanol and glycerol mixing it is equal
It is even, obtain culture medium siccative；
(2), water is added in culture medium siccative to be uniformly mixed, keeping water content is 70%, and adjusting pH value is 6.2, sterilizing
It is fitted into bacterium bag afterwards, obtains Medium for Ganoderma lucidum.
Comparative examples 3
The formula of Medium for Ganoderma lucidum
70 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts of wheat bran；4 parts of potassium dihydrogen phosphate；0.3 part of zinc sulfate；
2 parts of gypsum；0.6 part of triacontanol.
Comparative examples 4
The formula of Medium for Ganoderma lucidum
70 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts of wheat bran；4 parts of potassium dihydrogen phosphate；0.3 part of zinc sulfate；
Vitamin B10.2 part；2 parts of gypsum；3 parts of glycerol.
Detection method
Using the culture medium culture lucid ganoderma fungus in 1~embodiment of embodiment 5,1~comparative examples of comparative examples 4,
Ganoderma lucidum fruitbody is taken after ganoderma lucidum fruitbody generation, detects polyoses content therein, testing result table 1.
Table 1：Different embodiment cultivation results
| Embodiment classification | Polyoses content |
| Embodiment 3 | 1.2% |
| Comparative examples 1 | 0.27% |
| Comparative examples 2 | 0.58% |
| Comparative examples 3 | 0.97% |
| Comparative examples 4 | 0.85% |
Embodiment 3 is culture medium of the invention, ganoderma polyoses content highest in the ganoderma lucidum of culture.In comparative examples 1
The matrix for lacking ganoderma lucidum culture, contains only the FUKE QIANJIN PIAN dregs of a decoction, so that ganoderma lucidum grows required subalimentation.
The FUKE QIANJIN PIAN dregs of a decoction are not added in the culture medium of comparative examples 2, the ganoderma lucidum form of culture is poor compared with embodiment 3,
And when carrying out content detection to ganoderma lucidum, polyoses content is substantially less than embodiment 3, illustrates Medium for Ganoderma lucidum of the invention, is adding
After having entered the FUKE QIANJIN PIAN dregs of a decoction, the polyoses content in ganoderma lucidum fruitbody can be significantly improved, the intracorporal polysaccharide of ganoderma lucidum is improved and contains
Amount helps to improve the medical value of ganoderma lucidum.By the comparison of comparative examples 1~4 it is known that when reducing in culture medium
After fractions, the raising ability of polyoses content has different degrees of reduction, illustrates three kinds of medicinal materials to the function for improving polyoses content
Can go up has synergistic effect.
Claims (8)
1. a kind of Medium for Ganoderma lucidum, the culture medium is used to improve the content of ganoderma lucidum polysaccharide in ganoderma lucidum fruitbody, and culture medium includes
Following components by weight percent：
50 parts ~ 100 parts of the FUKE QIANJIN PIAN dregs of a decoction；
15 parts ~ 30 parts of wheat bran；1 part ~ 5 parts of potassium dihydrogen phosphate；0.1 part ~ 0.3 part of zinc sulfate；
Vitamin B10.1 part ~ 0.3 part；1 part ~ 3 parts of gypsum；0.1 part ~ 1 part of triacontanol；
1 part ~ 5 parts of glycerol.
2. culture medium as described in claim 1, it is characterised in that：Including following components by weight percent：
60 parts ~ 80 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts ~ 30 parts of wheat bran；3 parts ~ 5 parts of potassium dihydrogen phosphate；0.2 part ~ 0.3 part of zinc sulfate；
Vitamin B10.1 part ~ 0.25 part；2 parts ~ 3 parts of gypsum；0.5 part ~ 1 part of triacontanol；
3 parts ~ 5 parts of glycerol.
3. culture medium as claimed in claim 1 or 2, it is characterised in that：Including following components by weight percent：
70 parts of the FUKE QIANJIN PIAN dregs of a decoction；
25 parts of wheat bran；4 parts of potassium dihydrogen phosphate；0.3 part of zinc sulfate；
Vitamin B10.2 part；2 parts of gypsum；0.6 part of triacontanol；
3 parts of glycerol.
4. culture medium as described in claim 1, it is characterised in that：The FUKE QIANJIN PIAN dregs of a decoction are that FUKE QIANJIN PIAN raw material mentions
The dregs of a decoction after taking effective component.
5. the method for preparing any Medium for Ganoderma lucidum in claim 1 ~ 4, includes the following steps：
（1）, the FUKE QIANJIN PIAN dregs of a decoction are dried after crush, be added wheat bran stir evenly；Then potassium dihydrogen phosphate, sulfuric acid is added
Zinc, vitamin B1, gypsum, triacontanol and glycerol be uniformly mixed, obtain culture medium siccative；
（2）, in culture medium siccative be added water sterilize after mixing, be reloaded into bacterium bag, obtain Medium for Ganoderma lucidum.
6. method as claimed in claim 5, it is characterised in that：The partial size that the FUKE QIANJIN PIAN dregs of a decoction crush be 4mm ~
10mm。
7. method as claimed in claim 5, it is characterised in that：The water content of the Medium for Ganoderma lucidum is 70% ~ 80%.
8. method as claimed in claim 5, it is characterised in that：The pH value of the Medium for Ganoderma lucidum is 6 ~ 6.5.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102523928A (en) * | 2012-01-16 | 2012-07-04 | 广东省微生物研究所 | Method for culturing lucid ganoderma by using oral liquid dregs |
| CN104496688A (en) * | 2014-12-26 | 2015-04-08 | 南京中医药大学 | Lucid ganoderma solid cultivation medium and application of Mailuoning injection production waste in lucid ganoderma cultivation |
| CN104876706A (en) * | 2015-05-18 | 2015-09-02 | 张家港市鸿嘉数字科技有限公司 | Lucid ganoderma culture medium |
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2015
- 2015-09-21 CN CN201510603655.2A patent/CN105110858B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102523928A (en) * | 2012-01-16 | 2012-07-04 | 广东省微生物研究所 | Method for culturing lucid ganoderma by using oral liquid dregs |
| CN104496688A (en) * | 2014-12-26 | 2015-04-08 | 南京中医药大学 | Lucid ganoderma solid cultivation medium and application of Mailuoning injection production waste in lucid ganoderma cultivation |
| CN104876706A (en) * | 2015-05-18 | 2015-09-02 | 张家港市鸿嘉数字科技有限公司 | Lucid ganoderma culture medium |
Non-Patent Citations (2)
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| 中药渣用于培养灵芝菌及功能成分分析;陈合等;《农业工程学报》;20061230(第S2期);全文 * |
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