CN105557306B - A kind of method using dwarf lilyturf slag fermenting and producing medicinal fungal substance - Google Patents
A kind of method using dwarf lilyturf slag fermenting and producing medicinal fungal substance Download PDFInfo
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- CN105557306B CN105557306B CN201511006956.3A CN201511006956A CN105557306B CN 105557306 B CN105557306 B CN 105557306B CN 201511006956 A CN201511006956 A CN 201511006956A CN 105557306 B CN105557306 B CN 105557306B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a kind of methods using dwarf lilyturf slag fermenting and producing medicinal fungal substance.The invention is realized in this way:(1)Dwarf lilyturf slag is dried, is crushed, sieve pretreatment after it is spare;(2)It will be mixed in a certain ratio uniformly by dwarf lilyturf slag, water, nitrogen source and the phosphorus source of pretreatment;(3)Uniformly mixed material is subjected to steam sterilizing processing;(4)Mould is inoculated on material after sterilization and Ganoderma Lucidum carries out mixed culture solid state fermentation;(5)Fermentation culture medium just obtains medicinal fungal substance product after drying.Using the method for the invention, dwarf lilyturf slag can be converted into the medicinal fungal substance of unique function of odor.Dwarf lilyturf slag is carried out recycling by the present invention, is turned waste into wealth, and not only reduces its pollution on the environment, moreover it is possible to provide a kind of novel medicinal material for traditional Chinese medicine industry.
Description
Technical field
The invention belongs to trade waste recycling fields, are related to a kind of side using dwarf lilyturf slag fermenting and producing medicinal fungal substance
Method.
Background technology
Chinese medicine Radix Ophiopogonis is the dried root of liliaceous plant Radix Ophiopogonis, is a kind of traditional enriching yin Chinese medicine, and Chinese patent drug factory is by wheat
A large amount of dwarf lilyturf slag can be generated after the active ingredient extraction in winter, the modes such as generally use is burned, landfill are disposed it, this is not
It only needs to put into a large amount of fund, can also cause the waste and environmental pollution of resource.With the improvement of people's environmental awareness, occur
Dwarf lilyturf slag is carried out the technology of recycling by some.Chinese invention patent application No. is CN201310176881.8 discloses
" a kind of method using dwarf lilyturf slag fermenting and producing biological feedstuff ", the Chinese invention application No. is CN201210432126.7 is special
Profit is disclosed " a kind of method that soluble dietary fiber is prepared using dwarf lilyturf slag "." dwarf lilyturf slag solid state fermentation produces albumen to document
The dynamics research of feed "(Tan Xiandong, Liu Fengmei, Duan Yaning, sheep are dynamic according to golden dwarf lilyturf slags solid state fermentation production protein feed
Mechanics study [J] feed industries, 2012,23:31-34.)" fermentation dwarf lilyturf slag carries out the strain combination optimization of protein enrichment
Research "(Tan Xiandong, Wang Junjun, Yang Yijin, Duan Yaning, Hu Wei fermentation dwarf lilyturf slags carry out the strain combination optimization of protein enrichment
It studies [J] feeds to study, 2013,02:31-33.)Disclose the method using dwarf lilyturf slag solid state fermentation production protein feed.
Although the above method provides some thinkings for the recycling of dwarf lilyturf slag, over all, the processing of dwarf lilyturf slag and profit
With a still weak link, need further to conduct a research and promote new technology.
Invention content
Dwarf lilyturf slag fermenting and producing medicinal fungal substance is utilized it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of
Method, to widen the purposes of dwarf lilyturf slag, a Tiao Xin roads are opened up for the processing of dwarf lilyturf slag.
Method of the present invention using dwarf lilyturf slag fermenting and producing medicinal fungal substance, processing step are as follows successively:
1st, the pretreatment of dwarf lilyturf slag
It pulverizes and sieves after dwarf lilyturf slag is dried, the dwarf lilyturf slag for selecting grain size 0.3-0.45mm is spare.
2nd, dispensing and batch mixing
Will by pretreatment dwarf lilyturf slag, suitable nitrogen source,
Phosphorus source and water are uniformly mixed, and specific batching mode is as follows:
Take pretreated 100 parts of dwarf lilyturf slag, 3 ~ 5 parts of nitrogen source, 0.2 ~ 0.5 part of phosphorus source, first by nitrogen source and phosphorus source during dispensing
It is added to the water uniformly mixed, then prepared solution is uniformly mixed with dwarf lilyturf slag.The solid-state in prepared fermentation raw material
The mass ratio of material and water is 1:1.8~1:2.4.
3rd, it sterilizes
Mixture is subjected to sterilization treatment using autoclaving:Steam pressure 1atm, 121 DEG C of temperature, the time is extremely
It is 45 minutes less.
4th, it ferments
Mixture inoculation mould after sterilization treatment and ganoderma lucidum are subjected to mixed fungus fermentation, strain presses 10% ~ 20% total inoculation
Amount is inoculated with, fermentation condition:Pressure is normal pressure, and temperature is 28 DEG C ~ 30 DEG C, and fermentation period is 20 days ~ 22 days.
5th, it is dry
Fermentation culture medium is just obtained into medicinal fungal substance product after 60 DEG C of drying after fermentation.
In the method, when dispensing nitrogen source used be dusty yeast, one in groundnut meal, corn protein powder, soy meal
Kind is a variety of.
In the method, when dispensing phosphorus source used be potassium dihydrogen phosphate, one or both of dipotassium hydrogen phosphate.
In the method, the mould used in step 4 is one or both of trichoderma reesei, aspergillus fumigatus.
In the method, the ganoderma lucidum used in step 4 is one or both of red sesame, purple sesame.
The invention has the advantages that:
1. using the method for the invention, dwarf lilyturf slag can be converted into medicinal fungal substance, realize the resource of dwarf lilyturf slag
Change, not only reduced its pollution on the environment, but also a kind of novel medicinal material can be provided for traditional Chinese medicine industry.
2. compared with liquid state fermentation, the solid-state fermentation process that the present invention uses is with production equipment is simple, low energy consumption, production
Raw waste residue, the advantages such as waste water is few, environmental pollution is small, contribute to manufacturing enterprise to realize energy-saving and emission-reduction and clean manufacturing.
3. according to the method for the invention prepare medicinal fungal substance in containing protein, amino acid, alkaloid, vitamin,
Trace element, polysaccharide, triterpene, have adjust immune, anti-aging, antifatigue, antitumor, hypoglycemic, reducing blood lipid, protecting liver and detoxication,
Improve the multiple efficacies such as Myocardial Microcirculation.
4. being fermented by mould and ganoderma lucidum matched combined to dwarf lilyturf slag, the collaboration given full play between strain is made
With considerably improving the content of polysaccharide and triterpene compound in medicinal fungal substance.
Specific embodiment
1 the present embodiment of embodiment is using dwarf lilyturf slag as raw material, trichoderma reesei(Trichoderma reesei3.3711)With
Red sesame(Ganoderma lucidum5.00535)Solid state fermentation, which is carried out, for strain produces medicinal fungal substance.It is as follows:
1. the pretreatment of dwarf lilyturf slag
Dwarf lilyturf slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with dwarf lilyturf slag in easy crushing state.
By dried dwarf lilyturf slag grinding and sieving, grain size is selected to be used for subsequent technique for the dwarf lilyturf slag of 0.3 ~ 0.45mm;
2. dispensing and batch mixing
First 3.5g dusty yeasts, 0.25g potassium dihydrogen phosphates and the 100g Radix Ophiopogonis ground-slags by pre-processing are uniformly mixed, then
The solid material after mixing is uniformly mixed with 186.75g water again;
3. sterilizing
Mixture is subjected to sterilization treatment using autoclaving:Steam pressure 1atm, 121 DEG C of temperature, time is
45 minutes;
4. fermentation
Synchronous access trichoderma reesei in mixture after sterilization treatment(Trichoderma reesei 3.3711)With it is red
Sesame(Ganoderma lucidum5.00535)It ferments, trichoderma reesei(Trichoderma reesei3.3711)With it is red
Sesame(Ganoderma lucidum5.00535)Inoculation to compare be 1:2, total inoculum concentration is 15%, fermentation condition:Fermentation period is
21 days, pressure was normal pressure, and temperature is 28 DEG C;
5. drying
Product after fermentation just obtains medicinal fungal substance product through baking oven after normal pressure, 60 DEG C of dryings, in the product polysaccharide and
The content of triterpene compound is respectively 5.3% and 0.68%.
2 the present embodiment of embodiment is using dwarf lilyturf slag as raw material, aspergillus fumigatus(Aspergillus fumigatus3.8027)
And purple sesame(Ganoderma sinense5.00069)Solid state fermentation, which is carried out, for strain produces medicinal fungal substance.It is as follows:
1. the pretreatment of dwarf lilyturf slag
Dwarf lilyturf slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with dwarf lilyturf slag in easy crushing state.
By dried dwarf lilyturf slag grinding and sieving, grain size is selected to be used for subsequent technique for the dwarf lilyturf slag of 0.3 ~ 0.45mm;;
2. dispensing and batch mixing
First by 4g corn protein powders, 0.25g potassium dihydrogen phosphates, 0.2g dipotassium hydrogen phosphates and the 100g Radix Ophiopogonis by pretreatment
Ground-slag is uniformly mixed, and is then again uniformly mixed the solid material after mixing with 250.68g water;
3. sterilizing
Mixture is subjected to sterilization treatment using autoclaving:Steam pressure 1atm, 121 DEG C of temperature, time is
60 minutes;
4. fermentation
Aspergillus fumigatus is accessed in mixture after sterilization treatment(Aspergillus fumigatus3.8027)And purple sesame
(Ganoderma sinense5.00069)It ferments, aspergillus fumigatus(Aspergillus fumigatus3.8027)And purple
Sesame(Ganoderma sinense5.00069)Inoculation to compare be 1:1, total inoculum concentration is 20%, fermentation condition:Fermentation period is
20 days, pressure was normal pressure, and temperature is 30 DEG C;
5. drying
Product after fermentation just obtains medicinal fungal substance product through baking oven after normal pressure, 60 DEG C of dryings, in the product polysaccharide and
The content of triterpene compound is respectively 5.2% and 0.65%.
3 the present embodiment of embodiment is using dwarf lilyturf slag as raw material, trichoderma reesei(Trichoderma reesei3.3711)With
Purple sesame(Ganoderma sinense5.00069)Solid state fermentation, which is carried out, for strain produces medicinal fungal substance.It is as follows:
1. the pretreatment of dwarf lilyturf slag
Dwarf lilyturf slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with dwarf lilyturf slag in easy crushing state.
By dried dwarf lilyturf slag grinding and sieving, grain size is selected to be used for subsequent technique for the dwarf lilyturf slag of 0.3-0.45mm;
2. dispensing and batch mixing
First 5g dusty yeasts, 0.35g potassium dihydrogen phosphates and the 100g Radix Ophiopogonis ground-slags by pre-processing are uniformly mixed, Ran Houzai
Solid material after mixing is uniformly mixed with 252.84g water;
3. sterilizing
Mixture is subjected to sterilization treatment using autoclaving:Steam pressure 1atm, 121 DEG C of temperature, time is
60 minutes;
4. fermentation
Trichoderma reesei is accessed in mixture after sterilization treatment(Trichoderma reesei3.3711)And purple sesame
(Ganoderma sinense5.00069)It ferments, trichoderma reesei(Trichoderma reesei3.3711)And purple sesame
(Ganoderma sinense5.00069)Inoculation to compare be 1:1, total inoculum concentration is 20%, fermentation condition:Fermentation period is 22
My god, pressure is normal pressure, and temperature is 29 DEG C;
5. drying
Product after fermentation just obtains medicinal fungal substance product through baking oven after normal pressure, 60 DEG C of dryings, in the product polysaccharide and
The content of triterpene compound is respectively 5.5% and 0.68%.
4 the present embodiment of embodiment is using dwarf lilyturf slag as raw material, aspergillus fumigatus(Aspergillus fumigatus3.8027)
And red sesame(Ganoderma lucidum5.00706)Solid state fermentation, which is carried out, for strain produces medicinal fungal substance.It is as follows:
1. the pretreatment of dwarf lilyturf slag
Dwarf lilyturf slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with dwarf lilyturf slag in easy crushing state.
By dried dwarf lilyturf slag grinding and sieving, grain size is selected to be used for subsequent technique for the dwarf lilyturf slag of 0.3-0.45mm;
2. dispensing and batch mixing
First 2g dusty yeasts, 3g corn protein powders, 0.4g potassium dihydrogen phosphates and the 100g Radix Ophiopogonis ground-slags by pre-processing are mixed
It closes uniformly, is then again uniformly mixed the solid material after mixing with 231.88g water;
3. sterilizing
Mixture is subjected to sterilization treatment using autoclaving:Steam pressure 1atm, 121 DEG C of temperature, time is
75 minutes;
4. fermentation
Aspergillus fumigatus is accessed in mixture after sterilization treatment(Aspergillus fumigatus3.8027)And red sesame
(Ganoderma lucidum5.00706)It ferments, aspergillus fumigatus(Aspergillus fumigatus3.8027)With it is red
Sesame(Ganoderma lucidum5.00706)Inoculation to compare be 1:2, total inoculum concentration is 15%, fermentation condition:Fermentation period is
21 days, pressure was normal pressure, and temperature is 30 DEG C;
5. drying
Product after fermentation just obtains medicinal fungal substance product through baking oven after normal pressure, 60 DEG C of dryings, in the product polysaccharide and
The content of triterpene compound is respectively 5.2% and 0.67%.
5 the present embodiment of embodiment is using dwarf lilyturf slag as raw material, trichoderma reesei(Trichoderma reesei3.3711), it is purple
Sesame(Ganoderma sinense5.00069)And red sesame(Ganoderma lucidum5.00535)Solid-state is carried out for strain
Fermenting and producing medicinal fungal substance.It is as follows:
1. the pretreatment of dwarf lilyturf slag
Dwarf lilyturf slag is dried at normal pressure, 60 DEG C with baking oven first, the time is limited with dwarf lilyturf slag in easy crushing state.
By dried dwarf lilyturf slag grinding and sieving, grain size is selected to be used for subsequent technique for the dwarf lilyturf slag of 0.3 ~ 0.45mm;
2. dispensing and batch mixing
First 3.5g dusty yeasts, 0.35g dipotassium hydrogen phosphates and the 100g Radix Ophiopogonis ground-slags by pre-processing are uniformly mixed, then
The solid material after mixing is uniformly mixed with 207.7g water again;
3. sterilizing
Mixture is subjected to sterilization treatment using autoclaving:Steam pressure 1atm, 121 DEG C of temperature, time is
90 minutes;
4. fermentation
Synchronous access trichoderma reesei in mixture after sterilization treatment(Trichoderma reesei 3.3711), it is purple
Sesame(Ganoderma sinense5.00069)And red sesame(Ganoderma lucidum5.00535)It ferments, Richter scale wood
It is mould(Trichoderma reesei3.3711), purple sesame(Ganoderma sinense5.00069)And red sesame(Ganoderma lucidum5.00535)Inoculation to compare be 1: 1:1, total inoculum concentration is 15%, fermentation condition:Fermentation period is 21 days, and pressure is
Normal pressure, temperature are 29 DEG C;
5. drying
Product after fermentation just obtains medicinal fungal substance product through baking oven after normal pressure, 60 DEG C of dryings, in the product polysaccharide and
The content of triterpene compound is respectively 5.6% and 0.71%.
Claims (2)
1. the method that using dwarf lilyturf slag as raw material, medicinal fungal substance is produced by solid-state fermentation process for one kind, concrete technology feature is such as
Under:
(1)The pretreatment of dwarf lilyturf slag
It pulverizes and sieves after dwarf lilyturf slag is dried, the dwarf lilyturf slag for selecting grain size 0.3-0.45mm is spare;
(2)Dispensing and batch mixing
It will be uniformly mixed by dwarf lilyturf slag, suitable nitrogen source, phosphorus source and the water of pretreatment, specific batching mode is as follows:
Take pretreated 100 parts of dwarf lilyturf slag, 3 ~ 5 parts of nitrogen source, 0.2 ~ 0.5 part of phosphorus source, when dispensing first adds in nitrogen source and phosphorus source
It is uniformly mixed into water, then prepared solution is uniformly mixed with dwarf lilyturf slag;The solid material in prepared fermentation raw material
Mass ratio with water is 1:2.2~1:2.4;
(3)Sterilizing
Mixture is subjected to sterilization treatment using autoclaving:Steam pressure 1atm, 121 DEG C of temperature, the time is at least
45 minutes;
(4)Fermentation
By after sterilization treatment mixture inoculation mould and ganoderma lucidum carry out mixed fungus fermentation, strain by 10% ~ 20% total inoculum concentration into
Row inoculation, fermentation condition:Pressure is normal pressure, and temperature is 28 DEG C ~ 30 DEG C, and fermentation period is 20 days ~ 22 days;
(5)It is dry
Fermentation culture medium is just obtained into medicinal fungal substance product after 60 DEG C of drying after fermentation;
Nitrogen source used is one or more in dusty yeast, groundnut meal, corn protein powder, soy meal during dispensing;
Phosphorus source used is one or both of potassium dihydrogen phosphate, dipotassium hydrogen phosphate during dispensing;
The mould used during fermentation is one or both of trichoderma reesei, aspergillus fumigatus;
The ganoderma lucidum used during fermentation is one or both of red sesame, purple sesame.
2. the medicinal fungal substance that method obtains according to claim 1.
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CN112960998B (en) * | 2021-01-21 | 2023-05-02 | 台沃科技集团股份有限公司 | Fucheng ophiopogon japonicus resistant nutritional composition and application thereof |
CN112831480A (en) * | 2021-04-14 | 2021-05-25 | 成都信息工程大学 | Method for producing laccase by solid-state fermentation of pseudo-ginseng residue by lucid ganoderma |
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