CN102987097A - Method for producing complex enzyme preparation for feed by use of panax pseudoginseng slag fermentation - Google Patents
Method for producing complex enzyme preparation for feed by use of panax pseudoginseng slag fermentation Download PDFInfo
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- CN102987097A CN102987097A CN2012105213838A CN201210521383A CN102987097A CN 102987097 A CN102987097 A CN 102987097A CN 2012105213838 A CN2012105213838 A CN 2012105213838A CN 201210521383 A CN201210521383 A CN 201210521383A CN 102987097 A CN102987097 A CN 102987097A
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Abstract
The invention discloses a method for producing a complex enzyme preparation for feed by use of panax pseudoginseng slag fermentation. The method comprises the following steps of: (1) performing drying, grinding and screening pretreatment on panax pseudoginseng slag; (2) uniformly mixing the pretreated panax pseudoginseng slag, water and a nitrogen source according to a certain proportion; (3) performing steam sterilization on the uniformly mixed material; (4) inoculating aspergillus and trichoderma to the sterilized material for single-bacterium or mixed-bacterium solid state fermentation; and (5) drying and grinding the mixed-bacterium fermentation product to obtain a complex enzyme preparation for feed, and drying and grinding multiple single-bacterium fermentation products and mixing and compounding according to a certain proportion to obtain a complex enzyme preparation for feed. Through the method disclosed by the invention, the panax pseudoginseng slag can be converted into a complex enzyme preparation for feed capable of improving the utilization rate of feed and the digestive ability of livestock and poultry. The method realizes resource utilization of the panax pseudoginseng slag, turns waste into wealth, reduces environmental pollution and provides a new feed additive for the breeding industry.
Description
Technical field
The invention belongs to trade waste resource field, relate to a kind of method of utilizing pseudo-ginseng slag solid state fermentation to produce complex enzyme for feed.
Background technology
Pseudo-ginseng is China's tradition rare traditional Chinese medicine, also is a kind of medicinal and edible plant.The rhizome of pseudo-ginseng can be used as medicine, the main medicinal component of pseudo-ginseng is saponin, and the pseudo-ginseng slag behind the effective component extracting is disposed as discarded object usually, no matter is to adopt the means such as landfill, burning to dispose, or it as Fertilizer application, there is certain limitation.Still contain nutriment and the medicinal components such as starch, polysaccharide, protein, amino acid, alkaloid and various trace elements in the pseudo-ginseng slag after extracting total saponin, can will further develop as fermentation substrate it, not only can turn waste into wealth, improve the utilization ratio of resource, and offset except environmental pollution, it is significant to improve the ecological environment.Application number is that 201010232573.9 Chinese invention patent discloses " a kind of liquor and method with the fermenting and distilling pseudo-ginseng root dregs preparation "; Application number is that 201110119291.2 Chinese invention patent discloses " a kind of method with pseudo-ginseng slag fermentation production of protein feedstuff "; Application number is that 201210196021.6 Chinese invention patent discloses " a kind of method of utilizing the mould biological prevention and control agent of pseudo-ginseng slag fermenting and producing wood ".
Fodder enzyme preparation is a kind of dynamical bioactivator, along with the development and progress of biotechnology, fermentation industry and feed industry, makes people new cognition arranged and affirmed its effect in the animal husbandry and fishery aquaculture.In feed, add suitable fodder enzyme preparation, can improve utilization rate and the digestibility of feedstuff, promote digesting and assimilating of nutriment, and then improve breeding performonce fo animals, reduce excremental pollution.The fodder enzyme preparation kind is a lot, can be divided into single enzyme preparation and complex enzyme formulation two classes.The single enzyme preparation is divided into five classes basically: non-starch polysaccharide enzyme, phytase, amylase, protease and lipase.Complex enzyme formulation is main body by one or more single enzyme preparations, adds that other single enzyme preparations mix, or is obtained by one or more microbial fermentations.The feed enzyme preparation product that uses in the livestock and poultry cultivation at present both at home and abroad is take complex enzyme formulation as main, mainly comprises in cellulase, protease, amylase, carbohydrase, dextranase, zytase, the pectase one or more.Contain multiple boiomacromolecule nutriment in the feed for livestock, must behind enzymolysis, could be absorbed by livestock and poultry and utilize.Use the complex enzyme formulation multiple substrate that needs degraded in the feed of can degrading simultaneously, can improve to greatest extent the utilization rate of the nutriments such as starch in the feed, protein and cellulose, thereby reach the purpose that reduces food consumption.Therefore, from improving the feed resource utilization rate, aquaculture economic benefit, and the social benefit brought of the aspect such as environmental protection, fodder enzyme preparation is boundless in the application prospect of livestock and poultry breeding industry.
Raw material for the production of feeding enzyme in the prior art mainly contains various agricultural wastes, light industry grain slag; Although be also to contain a certain amount of feeding enzyme in the protein feed product of disclosed method preparation in 201110119291.2 the Chinese invention patent according to application number, but the main purpose of the method is in order to obtain the high thalline of protein content, the bacterial strain that plays in the method zymogenesis mainly is Aspergillus, saccharomycetic enzymatic productivity is very weak, and using saccharomycete in the sweat mainly is in order to gather in the crops thalline.Application number is that the bacterium producing multi enzyme preparation kind of using in 201110119291.2 the disclosed method of Chinese invention patent is less, and to the same substrate, it is outstanding that the most of microbe bacterial strain only has 1 enzyme to live, because the feedstuff comparison of ingredients is complicated, require to contain plurality of enzymes in the fodder enzyme preparation well its effect of performance.Therefore in the fodder enzyme preparation production process, need to adopt compounded technology to satisfy the fodder enzyme preparation product to the demand in multienzyme source, the following two kinds of compounded technologies of main use in this method: a plurality of single strains fermentations of the first, complementary composite algorithm, it is the enzyme system that every kind of microorganism has its characteristic, can obtain the enzyme source that multiple tool characteristic enzyme is after the fermentation separately, then complementary laminating production meets the product that prescription requires; It two is the mixed culture fermentation of many bacterial strains, and naturally complementary enzyme is method, is about to each bacterial strain combined inoculation, and one time fermentation forms and meets the product that prescription requires.
The present invention utilizes microorganism solid fermentation pseudo-ginseng slag to produce complex enzyme preparation for feeding, can also provide a kind of safe, nontoxic novel fodder additive for livestock breeding industry when realizing the industrial solid castoff minimizing.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing pseudo-ginseng slag solid state fermentation to produce complex enzyme preparation for feeding.The method of utilizing pseudo-ginseng slag solid state fermentation to produce complex enzyme preparation for feeding of the present invention, its processing step is as follows successively:
1, the preliminary treatment of pseudo-ginseng slag
With crushing screening after the wet pseudo-ginseng slag oven dry, select the pseudo-ginseng slag of particle diameter 0.1-0.8mm to be used for subsequent technique;
2, batching and batch mixing
To mix through pretreated pseudo-ginseng slag, an amount of nitrogenous source and water, concrete batching mode is as follows:
100 parts of pretreated pseudo-ginseng slags, nitrogenous source 2-5 part, the mass ratio of solid material and water is 1:1-1:2.5;
First nitrogenous source being added to the water during batching mixes, and solution and the pseudo-ginseng slag of preparing is mixed again;
3, sterilization
Adopt autoclaving to carry out sterilization treatment compound: steam pressure 1atm, 121 ℃ of temperature, the time is at least 30 minutes;
4, fermentation
Compound after the sterilization treatment is carried out mixed fungus fermentation, bacterial classification is inoculated by the inoculum concentration of 5%-20%, fermentation condition: pressure is normal pressure, temperature is 28 ℃-33 ℃, fermentation period is 3 days-8 days, at least use a kind of Aspergillus and a kind of Trichoderma during fermentation, fermentation mode can adopt single bacterium fermentation or mixed fungus fermentation;
5, dry and pulverizing
With the fermentation culture medium oven dry, then dried fermentation culture medium is pulverized, if in step 4, adopt the mixed fungus fermentation mode, can obtain the complex enzyme preparation for feeding product to this step;
6, composite
If in step 4, adopt single bacterium fermentation mode, then need pure-blood ferment culture dry, different strain after pulverizing is mixed composite, can obtain to meet the complex enzyme preparation for feeding product that prescription requires.
In the described method, used nitrogenous source is ammonium sulfate or urea during batching.
In the described method, the Aspergillus of using in the step 4 as aspergillus oryzae (
Aspergillus oryzae3.800), aspergillus niger (
Aspergillus niger3.4628), Aspergillus usamii (
Aspergillus usamii3.2926) in one or more.
In the described method, the Trichoderma of using in the step 4 as Trichoderma viride (
Trichoderma viride3.2942), healthy and free from worry wood mould (
Trichoderma koningii3.4033) in one or both.
The present invention has following beneficial effect:
1, adopts the method for the invention, the pseudo-ginseng slag can be converted into complex enzyme preparation for feeding, realized the resource of solid waste.
2, tunning and remaining Chinese medicine slag can all utilize, and do not have new generation of waste materials.
3, the complex enzyme preparation for feeding that adopts the present invention to produce contains the enzyme source that multiple tool characteristic enzyme is, helps to eliminate the ANFs in the feed, the utilization rate that improves feed and the digestion power of animal.
4, also contain various trace elements and medicinal component in this product, have certain health protection and disease prevention function.
The specific embodiment
Embodiment 1 present embodiment is take the pseudo-ginseng slag as raw material, aspergillus niger (
Aspergillus niger3.4628)/Trichoderma viride (
Trichoderma viride3.2942) for carrying out mixed culture solid state fermentation, bacterial classification produces complex enzyme preparation for feeding.Concrete steps are as follows:
1, the preliminary treatment of pseudo-ginseng slag
At first the pseudo-ginseng slag is dried with baking oven under normal pressure, 60 ℃, the time is easy pulverizing state with the pseudo-ginseng dregs of a decoction and is limited.With dried pseudo-ginseng slag grinding and sieving, select the pseudo-ginseng slag of particle diameter 0.3-0.5mm to be used for subsequent technique.
2, batching and batch mixing
First 3g ammonium sulfate is joined in the 160g water, mix, and then this solution and the pretreated 100g pseudo-ginseng ground-slag of process are mixed.
3, sterilization
Adopt autoclaving to carry out sterilization treatment compound: steam pressure 1atm, 121 ℃ of temperature, the time is at least 30 minutes.
4, fermentation
Adopt synchronous vaccination ways to ferment, access simultaneously in the compound after sterilization treatment aspergillus niger (
Aspergillus niger3.4628) and Trichoderma viride (
Trichoderma viride3.2942) ferment, the inoculation of aspergillus niger and Trichoderma viride is than being 1:1, and total inoculum concentration is 10%, and fermentation condition: pressure is normal pressure, and temperature is 30 ℃, fermentation period is 6 days.
5, dry and pulverizing
Fermentation culture medium in normal pressure, 50 ℃ of dryings, just obtains complex enzyme preparation for feeding through baking oven more after crushed.
Embodiment 2 present embodiments are take the pseudo-ginseng slag as raw material, respectively with aspergillus oryzae (
Aspergillus oryzae3.800), Aspergillus usamii (
Aspergillus usamii3.2926), healthy and free from worry wood mould (
Trichoderma koningii3.4033) for bacterial classification carries out single bacterium solid state fermentation, carry out again with three kinds of fermentation culture medium dryings, after pulverizing obtaining complex enzyme preparation for feeding after composite.Concrete steps are as follows:
1, the preliminary treatment of pseudo-ginseng slag
At first the pseudo-ginseng slag is dried with baking oven under normal pressure, 60 ℃, the time is easy pulverizing state with the pseudo-ginseng dregs of a decoction and is limited.With dried pseudo-ginseng slag grinding and sieving, select the pseudo-ginseng slag of particle diameter 0.2-0.4mm to be used for subsequent technique.
2, batching and batch mixing
First 3.5g ammonium sulfate is joined in the 190g water, mix, and then this solution and the pretreated 100g pseudo-ginseng ground-slag of process are mixed.
3, sterilization
Adopt autoclaving to carry out sterilization treatment compound: steam pressure 1atm, 121 ℃ of temperature, the time is at least 30 minutes.
4, fermentation
Get 3 parts according to the fermentation mediums of the described method of step 1-step 3 preparation, access respectively aspergillus oryzae (
Aspergillus oryzae3.800), Aspergillus usamii (
Aspergillus usamii3.2926) and healthy and free from worry wood mould (
Trichoderma koningii3.4033) carry out single bacterium fermentation, inoculum concentration is 10%, fermentation condition: pressure is normal pressure, and temperature is 30 ℃, and fermentation period was respectively 5 days, 5 days and 6 days.
5, dry and pulverizing
Use first baking oven in normal pressure, 50 ℃ of dryings single bacterium fermentation culture medium of above-mentioned 3 kinds of moulds, then pulverize, mix according to the mass ratio of 1:1:1 again and just obtain complex enzyme preparation for feeding.
Embodiment 3 present embodiments are take the pseudo-ginseng slag as raw material, Aspergillus usamii (
Aspergillus usamii3.2926), healthy and free from worry wood mould (
Trichoderma koningii3.4033) for carrying out mixed culture solid state fermentation, bacterial classification produces complex enzyme preparation for feeding.Concrete steps are as follows:
1, the preliminary treatment of pseudo-ginseng slag
At first the pseudo-ginseng slag is dried with baking oven under normal pressure, 60 ℃, the time is easy pulverizing state with the pseudo-ginseng dregs of a decoction and is limited.With dried pseudo-ginseng slag grinding and sieving, select the pseudo-ginseng slag of particle diameter 0.4-0.6mm to be used for subsequent technique.
2, batching and batch mixing
First 3.5g ammonium sulfate is joined in the 200g water, mix, and then this solution and the pretreated 100g pseudo-ginseng ground-slag of process are mixed.
3, sterilization
Adopt autoclaving to carry out sterilization treatment compound: steam pressure 1atm, 121 ℃ of temperature, the time is at least 30 minutes.
4, fermentation
Adopt synchronous vaccination ways to ferment, access simultaneously in the compound after sterilization treatment Aspergillus usamii (
Aspergillus usamii3.2926) and healthy and free from worry wood mould (
Trichoderma koningii3.4033) ferment, the mould inoculation ratio of Aspergillus usamii and healthy and free from worry wood is 1:2, and total inoculum concentration is 15%, and fermentation condition: pressure is normal pressure, and temperature is 30 ℃, fermentation period is 6 days.
5, dry and pulverizing
Fermentation culture medium in normal pressure, 50 ℃ of dryings, just obtains complex enzyme preparation for feeding through baking oven more after crushed.
Claims (5)
1. one kind take the pseudo-ginseng slag as raw material, and by the method that solid-state fermentation process is produced complex enzyme preparation for feeding, its concrete technology feature is as follows:
(1) preliminary treatment of pseudo-ginseng slag
With crushing screening after the wet pseudo-ginseng slag oven dry, select the pseudo-ginseng slag of particle diameter 0.1-0.8mm to be used for subsequent technique;
(2) batching and batch mixing
To mix through pretreated pseudo-ginseng slag, an amount of nitrogenous source and water, concrete batching mode is as follows:
100 parts of pretreated pseudo-ginseng slags, nitrogenous source 2-5 part, the mass ratio of solid material and water is 1:1-1:2.5;
First nitrogenous source being added to the water during batching mixes, and solution and the pseudo-ginseng slag of preparing is mixed again;
(3) sterilization
Adopt autoclaving to carry out sterilization treatment compound: steam pressure 1atm, 121 ℃ of temperature, the time is at least 30 minutes;
(4) fermentation
Compound after the sterilization treatment is carried out mixed fungus fermentation, bacterial classification is inoculated by the inoculum concentration of 5%-20%, fermentation condition: pressure is normal pressure, temperature is 28 ℃-33 ℃, fermentation period is 3 days-8 days, at least use a kind of Aspergillus and a kind of Trichoderma during fermentation, fermentation mode can adopt single bacterium fermentation or mixed fungus fermentation;
(5) dry and pulverizing
With the fermentation culture medium oven dry, then dried fermentation culture medium is pulverized, if in step 4, adopt the mixed fungus fermentation mode, can obtain the complex enzyme preparation for feeding product to this step;
(6) composite
If in step 4, adopt single bacterium fermentation mode, then need pure-blood ferment culture dry, different strain after pulverizing is mixed composite, can obtain to meet the complex enzyme preparation for feeding product that prescription requires.
2. method according to claim 1, used nitrogenous source is ammonium sulfate or urea when it is characterized in that preparing burden.
3. method according to claim 1, the Aspergillus of using when it is characterized in that fermenting as aspergillus oryzae (
Aspergillus oryzae3.800), aspergillus niger (
Aspergillus niger3.4628), Aspergillus usamii (
Aspergillus usamii3.2926) in one or more.
4. method according to claim 1, the Trichoderma of using when it is characterized in that fermenting as Trichoderma viride (
Trichoderma viride3.2942), healthy and free from worry wood mould (
Trichoderma koningii3.4033) in one or both.
5. the complex enzyme preparation for feeding that obtains of the described method of one of claim 1-4.
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| CN103156066A (en) * | 2013-04-02 | 2013-06-19 | 叶文明 | Feed taking desert cistanche drag as raw materials and preparation method thereof |
| CN103493681A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing medical mycoplasm by using pseudo-ginseng dregs in co-fermentation mode |
| CN105028908A (en) * | 2015-08-11 | 2015-11-11 | 新疆石河子职业技术学院 | Licorice root residue protein feed and preparation method thereof |
| CN105831422A (en) * | 2016-04-08 | 2016-08-10 | 重庆工商大学 | A preparing method of a feed additive boosting animal immunity |
| CN106581310A (en) * | 2016-12-15 | 2017-04-26 | 北京同力兴科农业科技有限公司 | Traditional Chinese medicine micro-ecological preparation for feed, and preparation method and application thereof |
| CN109770056A (en) * | 2018-11-29 | 2019-05-21 | 北京生泰尔科技股份有限公司 | A kind of Chinese medicine residue degradation bacteria and its preparing the application in poultry fodder additive |
| CN112890025A (en) * | 2021-03-01 | 2021-06-04 | 黑龙江省科学院微生物研究所 | Method for preparing silage by combining mold and lactic acid bacteria for producing cellulase |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156066A (en) * | 2013-04-02 | 2013-06-19 | 叶文明 | Feed taking desert cistanche drag as raw materials and preparation method thereof |
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| CN103493681A (en) * | 2013-09-24 | 2014-01-08 | 成都信息工程学院 | Method for producing medical mycoplasm by using pseudo-ginseng dregs in co-fermentation mode |
| CN105028908A (en) * | 2015-08-11 | 2015-11-11 | 新疆石河子职业技术学院 | Licorice root residue protein feed and preparation method thereof |
| CN105831422A (en) * | 2016-04-08 | 2016-08-10 | 重庆工商大学 | A preparing method of a feed additive boosting animal immunity |
| CN106581310A (en) * | 2016-12-15 | 2017-04-26 | 北京同力兴科农业科技有限公司 | Traditional Chinese medicine micro-ecological preparation for feed, and preparation method and application thereof |
| CN109770056A (en) * | 2018-11-29 | 2019-05-21 | 北京生泰尔科技股份有限公司 | A kind of Chinese medicine residue degradation bacteria and its preparing the application in poultry fodder additive |
| CN112890025A (en) * | 2021-03-01 | 2021-06-04 | 黑龙江省科学院微生物研究所 | Method for preparing silage by combining mold and lactic acid bacteria for producing cellulase |
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Application publication date: 20130327 |