CN103229891B - Method for producing protein feed by using cynomorium songaricum residues - Google Patents
Method for producing protein feed by using cynomorium songaricum residues Download PDFInfo
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- 241000804384 Cynomorium songaricum Species 0.000 title claims abstract description 48
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 34
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 34
- 238000004519 manufacturing process Methods 0.000 title abstract description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 30
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 20
- 241000196324 Embryophyta Species 0.000 claims abstract description 11
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 11
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 11
- 239000011734 sodium Substances 0.000 claims abstract description 11
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 11
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 11
- 239000011780 sodium chloride Substances 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 150000001875 compounds Chemical class 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 13
- 239000002893 slag Substances 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 239000002956 ash Substances 0.000 claims description 5
- 108010089934 carbohydrase Proteins 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 239000002023 wood Substances 0.000 claims description 5
- 229930010555 Inosine Natural products 0.000 claims description 4
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical class [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 4
- 235000013312 flour Nutrition 0.000 claims description 4
- 229960003786 inosine Drugs 0.000 claims description 4
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 4
- 235000019629 palatability Nutrition 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 239000003814 drug Substances 0.000 abstract description 9
- 244000144972 livestock Species 0.000 abstract description 4
- 244000144977 poultry Species 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 239000000470 constituent Substances 0.000 abstract 2
- 238000009776 industrial production Methods 0.000 abstract 1
- 229940126680 traditional chinese medicines Drugs 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 4
- 238000010563 solid-state fermentation Methods 0.000 description 4
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 229940073490 sodium glutamate Drugs 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 108010027322 single cell proteins Proteins 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007905 drug manufacturing Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000013586 microbial product Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Fodder In General (AREA)
Abstract
The invention discloses a method for producing a protein feed by using cynomorium songaricum residues, and relates to a method for producing a protein feed by using traditional Chinese medicine residues, belonging to the field of utilization of traditional Chinese medicine residues. The protein feed is prepared from the following raw materials by weight percent: 94-96% of pretreated cynomorium songaricum residues, 5-6% of NaCl, 0.01% of plant ash, 0.025% of NaOH, 0.05% of sodium humate and 0.01% of sodium bicarbonate. The method has the beneficial effect of converting the waste cynomorium songaricum residues into the protein feed so as to decrease the pollution caused to the environment and providing a low-cost feed to the livestock and poultry; the cynomorium songaricum residues still contain a little effective constituents of traditional Chinese medicines, so that after the livestock and poultry eat the cynomorium songaricum residues, the effect of preventing and curing diseases is provided and the residual effective constituents in the residues are reasonably and fully utilized; and the method is simple in process, short in production cycle and beneficial for industrial production.
Description
Technical field
The present invention relates to a kind of method of producing protein feed with Chinese medicine slag, belong to the field that utilizes of Chinese medicine slag, specifically a kind of cynomorium songaricum slag is produced the method for protein feed.
Background technology
Solid state fermentation refers to utilize nature substrate to do carbon source and the energy, or utilizes inert substrate to do solid support, and its system is anhydrous or approach anhydrous any sweat, and microbial growth and the product that forms are all in stromal surface.
Application solid-state fermentation technology is produced protein feed, single cell protein and mycoprotein, the forage protein source of a large amount of high-qualitys not only can be provided, and can also develop the feed addictive with specific function, as microbial products such as active dry yeasr, active bacteria formulation, probiotics.Along with the development of animal husbandry, the demand of feed is originally larger.And the deficiency of Feed Manufacturing amount, especially protein feed has become global problems.In the face of the situation of feed resource critical shortage, in the urgent need to developing new protein feed resources.In protein feed develops, all take much count of the research of single cell protein both at home and abroad, actively utilize crop material to produce protein feed, meet the demand of feed industry, aquaculture, alleviate feed imbalance between supply and demand, the while also can solve straw, the dregs of a decoction burn the problem of environmental pollution causing.
The traditional Chinese medical science is the rarity in China's medical treasure-house, and but then, Chinese medicine used can produce a lot of Chinese medicine slags.Especially Chinese patent drug processing enterprise, must follow the appearance of a large amount of Chinese medicine slags in its drug production process, if process not in time, very easily rot smelly, to environment.
Along with the development of agricultural biotechnologies, with biotechnology particularly microorganism fermentation means process the dregs of a decoction, become feed stripped source, not only can promote the development of China's animal husbandry, and can improve agroecological environment, and realize making full use of of resource, turn waste into wealth.By market survey, the at present domestic research of carrying out solid-state fermentation protein feed stuff is fresh is report.The production line that carries out the solid-state fermentation protein feed stuff of state's mould industrialization production has become China's Agricultural Bio-industry Developmentization problem in the urgent need to address.
Summary of the invention
Object object of the present invention is to overcome the deficiencies in the prior art, provides a kind of cynomorium songaricum slag to produce the method for protein feed, to widen the purposes of the cynomorium songaricum dregs of a decoction, the residue nutritional labeling in the dregs of a decoction is adequately and reasonably utilized.
For achieving the above object, a kind of cynomorium songaricum slag of the present invention is produced the method for protein feed, it is characterized in that: its processing step is as follows successively:
One, first the cynomorium songaricum dregs of a decoction are dried and make it be easy pulverizing state, then the dried cynomorium songaricum dregs of a decoction being crushed to granularity is 1.0～2.Omm;
Two, the mass ratio of solid matter and water is 2:1.8～1:1, solid matter is made up of the raw material of following weight proportion: the pretreated cynomorium songaricum dregs of a decoction 94～96%, NaCl 5～6%, plant ash 0.01%, NaOH 0.025%, sodium humate 0.05%, sodium bicarbonate 0.01%, above-mentioned NaCl, plant ash, NaOH, sodium humate, sodium bicarbonate are added to the water and are mixed, prepared solution is mixed with the pretreated cynomorium songaricum dregs of a decoction, the pH value of compound is controlled at 4.5～6.0 again;
Three, compound step 2 being made carries out sterilization treatment, and sterilizing adopts autoclaving, 1 standard atmospheric pressure of steam pressure, and temperature is to process 25～45min at 121 DEG C;
Four, the compound after sterilization treatment is fermented, bacterial classification is inoculated by 6～12% inoculum concentrations, fermentation condition: pressure is normal pressure, and temperature is 30～37 DEG C, and fermentation period is 4～7 days;
Five, be drying to obtain cynomorium songaricum residue protein feed, water content < 10% after having fermented.
Described dry mode has multiple, but preferably adopts the mode of drying, 45～60 DEG C of temperature, and pressure is normal pressure.
In described method, the bacterial classification of using when fermentation is by following weight proportion: carbohydrase: healthy and free from worry wood is mould: yeast cake powder=1:1:1.
In order to improve the palatability of cynomorium songaricum residue protein feed and the content of nutritional labeling, when batching, in every kilogram of cynomorium songaricum residue protein feed, add sodium glutamate 100～200g, inosine acid disodium 100～150g, corn flour 50～200g.
A kind of cynomorium songaricum slag of the present invention is produced the method for protein feed, its beneficial effect is: adopt the method for the invention, the discarded object cynomorium songaricum dregs of a decoction can be converted into protein feed, both reduce its pollution on the environment, can provide feed cheaply for livestock and poultry again; And owing to still containing a small amount of middle the effective elements of the medicine in the cynomorium songaricum dregs of a decoction, after therefore livestock and poultry eat, there is the effect of preventing and curing diseases, rationally and fully utilized the residue active ingredient in the dregs of a decoction; Technique of the present invention is simple, with short production cycle, is conducive to industrialization and produces.
Detailed description of the invention
Following examples are that the present invention further goes out to set forth.
Embodiment 1
One, first the cynomorium songaricum dregs of a decoction are dried, adopt the mode of drying, temperature 45 C, pressure is normal pressure, makes it be easy pulverizing state, then the dried cynomorium songaricum dregs of a decoction being crushed to granularity is 1.0mm;
Two, the mass ratio of solid matter and water is 2:1.8, solid matter is made up of the raw material of following weight proportion: pretreated cynomorium songaricum dregs of a decoction 96kg, NaCl 3.9kg, plant ash 0.01kg, NaOH 0.03kg, sodium humate 0.05kg, sodium bicarbonate 0.01kg, above-mentioned NaCl, plant ash, NaOH, sodium humate, sodium bicarbonate are added to the water and are mixed, prepared solution is mixed with the pretreated cynomorium songaricum dregs of a decoction, the pH value of compound is controlled at 4.5 again;
Three, compound step 2 being made carries out sterilization treatment, and sterilizing adopts autoclaving, 1 standard atmospheric pressure of steam pressure, and temperature is to process 25min at 121 DEG C;
Four, the compound after sterilization treatment is fermented, bacterial classification is mould 2% by carbohydrase 2%, healthy and free from worry wood, yeast cake powder 2% inoculum concentration is inoculated, fermentation condition: pressure is normal pressure, and temperature is 30 DEG C, fermentation period is 4 days;
Five, be dried after having fermented, adopt the mode of drying, temperature 45 C, pressure is that normal pressure obtains cynomorium songaricum residue protein feed, water content < 10%.
Embodiment 2
One, first the cynomorium songaricum dregs of a decoction are dried, adopt the mode of drying, temperature 45 C, pressure is normal pressure, makes it be easy pulverizing state, then the dried cynomorium songaricum dregs of a decoction being crushed to granularity is 1.0mm;
Two, the mass ratio of solid matter and water is 2:1.8, solid matter is made up of the raw material of following weight proportion: pretreated cynomorium songaricum dregs of a decoction 96kg, NaCl 3.9kg, plant ash 0.01kg, NaOH 0.03kg, sodium humate 0.05kg, sodium bicarbonate 0.01kg, above-mentioned NaCl, plant ash, NaOH, sodium humate, sodium bicarbonate are added to the water and are mixed, prepared solution is mixed with the pretreated cynomorium songaricum dregs of a decoction, the pH value of compound is controlled at 4.5 again;
Three, compound step 2 being made carries out sterilization treatment, and sterilizing adopts autoclaving, 1 standard atmospheric pressure of steam pressure, and temperature is to process 25min at 121 DEG C;
Four, the compound after sterilization treatment is fermented, bacterial classification is mould 2% by carbohydrase 2%, healthy and free from worry wood, yeast cake powder 2% inoculum concentration is inoculated, fermentation condition: pressure is normal pressure, and temperature is 30 DEG C, fermentation period is 4 days;
Five, be dried after having fermented, adopt the mode of drying, temperature 45 C, it is for subsequent use that pressure is that normal pressure obtains cynomorium songaricum residue protein feed, adds sodium glutamate 100, inosine acid disodium 100, corn flour 50 and make when batching in every kilogram of cynomorium songaricum residue protein feed.
Embodiment 3
One, first the cynomorium songaricum dregs of a decoction are dried and make it be easy pulverizing state, then the dried cynomorium songaricum dregs of a decoction being crushed to granularity is 2.Omm;
Two, the mass ratio of solid matter and water is 1:1, solid matter is made up of the raw material of following weight proportion: pretreated cynomorium songaricum dregs of a decoction 94kg, NaCl 5.9kg, plant ash 0.01kg, NaOH 0.03kg, sodium humate 0.05kg, sodium bicarbonate 0.01kg, above-mentioned NaCl, plant ash, NaOH, sodium humate, sodium bicarbonate are added to the water and are mixed, prepared solution is mixed with the pretreated cynomorium songaricum dregs of a decoction, the pH value of compound is controlled at 6.0 again;
Three, compound step 2 being made carries out sterilization treatment, and sterilizing adopts autoclaving, 1 standard atmospheric pressure of steam pressure, and temperature is to process 45min at 121 DEG C;
Four, the compound after sterilization treatment is fermented, bacterial classification is mould 3% by carbohydrase 3%, healthy and free from worry wood, yeast cake powder 3% inoculum concentration is inoculated, fermentation condition: pressure is normal pressure, and temperature is 37 DEG C, fermentation period is 7 days;
Five, be drying to obtain cynomorium songaricum residue protein feed, water content < 10% after having fermented.
In order to improve the palatability of cynomorium songaricum residue protein feed and the content of nutritional labeling, when batching, in every kilogram of cynomorium songaricum residue protein feed, add sodium glutamate 200g, inosine acid disodium 150g, corn flour 200g.
Experiment: (a kind of cynomorium songaricum slag of the present invention is produced protein feed) impact on pig feed intake
1. experimental design:
animal used as test and grouping: choose the health pig of the about 18kg of 80 body weight left and right, be divided into immediately 4 groups, 20, every group of two hurdle;
feeding and management: 30d is carried out in experiment, raises by pig farm usual manner.Experiment pig stable breeding.Every hurdle is raised 15, every gift morning, feeds once afternoon, and siccative powder free choice feeding, freely drinks water.When experiment starts and finishes, every group is carried out individuality and weighs, statistics feed intake.
The impact of table 1 on pig production performance
| ? | Starting weight (kg) | End heavy (kg) | Weightening finish (kg) | Average daily gain (g) | Head is feed consumption g/D all | Material anharmonic ratio |
| Control group | 18.17 | 31.65 | 13.48 | 449 | 1093 | 2.43 |
| Experimental group | 17.89 | 32.78 | 14.89 | 496 | 1187 | 2.39 |
Learn according to experimental data: control group and experimental group relatively in, control group weightening finish 13.48kg; Experimental group weightening finish 14.89kg, experimental group is higher than control group, and per day weightening finish is also higher than control group, and therefore, a kind of cynomorium songaricum slag of the present invention is produced protein feed can replace conventional feed, and improves animal to the digesting and assimilating of nutriment, and promotes growth of animal.
Claims (1)
1. cynomorium songaricum slag is produced a method for protein feed, it is characterized in that: its processing step is as follows successively:
1. first the cynomorium songaricum dregs of a decoction are dried and make it be easy pulverizing state, then the dried cynomorium songaricum dregs of a decoction being crushed to granularity is 1.0～2.0mm;
2. the mass ratio of solid matter and water is 2:1.8～1:1, solid matter is made up of the raw material of following weight proportion: the pretreated cynomorium songaricum dregs of a decoction 94～96%, NaCl5～6%, plant ash 0.01%, NaOH0.025%, sodium humate 0.05%, sodium bicarbonate 0.01%, above-mentioned NaCl, plant ash, NaOH, sodium humate, sodium bicarbonate are added to the water and are mixed, prepared solution is mixed with the pretreated cynomorium songaricum dregs of a decoction, the pH value of compound is controlled at 4.5～6.0 again;
3. compound step 2 being made carries out sterilization treatment, and sterilizing adopts autoclaving, 1 standard atmospheric pressure of steam pressure, and temperature is to process 25～45min at 121 DEG C;
4. the compound after sterilization treatment is fermented, bacterial classification is inoculated by 6～12% inoculum concentrations, fermentation condition: pressure is normal pressure, and temperature is 30～37 DEG C, and fermentation period is 4～7 days;
5. after having fermented, be drying to obtain cynomorium songaricum residue protein feed;
6. for increasing palatability, 100 grams of sodium glutamates, 100 grams of inosine acid disodiums, 50 grams of corn flour will be added in above-mentioned every kilogram of cynomorium songaricum residue protein feed;
In described method, the bacterial classification of using when fermentation is by following weight proportion: carbohydrase: healthy and free from worry wood is mould: yeast cake powder=1:1:1.
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| CN104012801A (en) * | 2014-06-11 | 2014-09-03 | 湖南中医药大学 | Poultry feed containing callicarpa nudiflora and preparing method of poultry feed |
| CN104012767A (en) * | 2014-06-11 | 2014-09-03 | 湖南中医药大学 | Poultry feed containing angelica sinensis and preparation method and applications thereof |
| CN104012768A (en) * | 2014-06-11 | 2014-09-03 | 湖南中医药大学 | Livestock and poultry feed containing decoction of four noble drugs, and preparation method and application thereof |
| CN104171289B (en) * | 2014-07-03 | 2017-05-17 | 甘肃天水岐黄药业有限责任公司 | Method for preparing bacterial protein from desertliving cistanche dregs |
| CN108041284A (en) * | 2017-12-19 | 2018-05-18 | 广西中医药大学附属瑞康医院 | A kind of method of Chinese medicine slag production protein feed |
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