CN102643747A - Method for preparing probiotics mixed preparation for processing straws - Google Patents
Method for preparing probiotics mixed preparation for processing straws Download PDFInfo
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- CN102643747A CN102643747A CN2012101440813A CN201210144081A CN102643747A CN 102643747 A CN102643747 A CN 102643747A CN 2012101440813 A CN2012101440813 A CN 2012101440813A CN 201210144081 A CN201210144081 A CN 201210144081A CN 102643747 A CN102643747 A CN 102643747A
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Abstract
A method for preparing a probiotics mixed preparation for processing straws belongs to the technology of microorganism solid-liquid fermentation and includes: respectively inoculating candida utilis, saccharomycopsis fibuligera, bacillus subtilis, pediococcus pentosaceus and trichoderma koningii strains into respective seed culture media, culturing for 24-132 hours at the condition of ventilation at the temperature of 28-32 DEG C, respectively inoculating mature seeds into fermentation media prepared by corn saccharification liquid, and fermenting for 24-96 hours under the ventilation condition at the temperature of 32+/-1DEG C, and adding bran into the mature seeds for cooling and drying to preparing microbial inoculum, wherein the inoculating amount is 10%. The method is simple in process, the probiotics mixed preparation can remarkably improve nutrition quality of straw feed, is good in feeding palatability and high in digestibility, and feeding cost is reduced.
Description
Technical field
The invention belongs to mikrobe solid-liquid fermentation technique, relate generally to a kind of probiotic bacterium mixed preparation preparation method who handles stalk.
Background technology
China is large agricultural country, and agricultural straw resource is extremely abundant, produces agricultural crop straw per year and reaches 900,000,000 tons.But; Because it is still immature that the livestock feed technology is processed into the agricultural crop straw processing in present China; Agricultural crop straw is made into the utilization ratio less than 10% of feed, and the straw feed that utilizes prior art to transform to manufacture is of low quality, and it is poor to feed the palatability of raising; But straw nutrition composition transformation efficiency and livestock digestibility are low, have influenced feeding of livestock and have raised effect.
Summary of the invention
The object of the invention is exactly the problem that exists to above-mentioned prior art; Straw resource utilization and livestock rearing industry development need in conjunction with China's abundant; Research provides a kind of probiotic bacterium mixed preparation preparation method who handles stalk, reaches its mixed preparation and can improve the agricultural crop straw feeding quality, reduce feed and to raise cost, promote the purpose that livestock rearing already develops.
The objective of the invention is to realize like this: a kind of probiotic bacterium mixed preparation preparation method who handles stalk; Said preparing method's step is following: Candida utilis, saccharomycopsis fibuligera, subtilis, Pediococcus pentosaceus, healthy and free from worry Trichoderma kind are inoculated into respectively in the seed culture medium separately, under 28-32 ℃, ventilation condition, cultivated 24-132 hour; Be inoculated into respectively in the fermention medium by the preparation of corn saccharification liquid cultivating sophisticated seed, its inoculum size was 10%, 32 ± 1 ℃, ventilation condition bottom fermentation 24-96 hour; To cultivate sophisticated seed and add wheat bran and carry out freezingly, dry, must handle the probiotic bacterium mixed preparation microbial inoculum product of stalk.
The Candida utilis that the present invention selected for use, saccharomycopsis fibuligera, subtilis, Pediococcus pentosaceus, healthy and free from worry Trichoderma bacterial classification are all bought in the commercial market.
The present invention is through the method for multi-strain fermentation, and preparation can be handled the fermenting agent of stalk, is a comprehensive utilization of waste materials, the optimal path that improves added value of product.The thalline microbial inoculum of the present invention's preparation can be with the ester bond generation enzymolysis of the robust fibre in the stalk (Mierocrystalline cellulose, semicellulose), xylogen, the long molecular chain of xylan, lignifying compound; Transform into the low molecular weight carbohydrate that can absorb to the nonabsorbable polymeric carbohydrate of animal, i.e. the energy feed; Multiple microorganism live bacteria can be drawn organonitrogen, the inorganic nitrogen that animal is difficult to utilize in a large number, makes it to change into the higher multiple bacterium protein of nutritive ingredient, i.e. protein fodder; Multiple microorganism live bacteria can produce a large amount of proteolytic enzyme, lypase, glycase, cellulolytic enzyme, vitamin B group and A, D VITAMINs in fermentation; Multiple microorganism live bacteria is set up microecological balance in animal body, has strengthened immunizing power.The present invention still solves the important channel of thalline inadequate resource.Breed through microbial fermentation, a large amount of somatic cells, synthesized a large amount of thalline and useful meta-bolites simultaneously, these thalline all are the fine thalline.Main raw material of the present invention is inexpensive being easy to get such as wheat bran, Semen Maydis powder, after fermentation, improves its nutritive value and economic worth greatly.
Embodiment
Be described in detail in the face of embodiment of the present invention down.A kind of probiotic bacterium mixed preparation preparation method who handles stalk; Said preparing method's step is following: Candida utilis, saccharomycopsis fibuligera, subtilis, Pediococcus pentosaceus, healthy and free from worry Trichoderma kind are inoculated into respectively in the seed culture medium separately, under 28-32 ℃, ventilation condition, cultivated 24-132 hour; Be inoculated into respectively in the fermention medium by the preparation of corn saccharification liquid cultivating sophisticated seed, its inoculum size was 10%, 32 ± 1 ℃, ventilation condition bottom fermentation 24-96 hour; To cultivate sophisticated seed and add wheat bran and carry out freezingly, dry, must handle the probiotic bacterium mixed preparation microbial inoculum product of stalk.
The mould seed culture medium of described Pediococcus pentosaceus and healthy and free from worry wood is made up of 50 gram straw powder, 10 gram wheat bran and 10 gram ammonium sulfate; When manufacturing; With 50 gram straw powder, 10 gram wheat bran and 10 gram ammonium sulfate mix mixture, in mixture, add 1000 ml water solution, stir; The mixture aqueous solution is poured into 60 milliliters respectively in the triangular flask of 250 milliliters of volumes; Triangular flask is placed shaking table, under 32 ℃, 120 rev/mins conditions, cultivated 132 hours, obtain seed culture medium.
The seed culture medium of Candida utilis, saccharomycopsis fibuligera and subtilis: get Semen Maydis powder 20 grams, add water to 100 milliliters of mixings, regulate pH to 6.8 with NaOH and be heated to 90 ℃; Ratio in 10 units/gram Semen Maydis powders adds AMS, continues insulation 25-35 minute, adds saccharifying enzyme by 20 units/gram Semen Maydis powders thereafter; After being incubated 4 hours under 65 ℃ of conditions; Under 121 ℃ of temperature sterilising treatment 10-15 minute, be cooled to 30 ℃ subsequent use, obtain seed culture medium.
Claims (2)
1. probiotic bacterium mixed preparation preparation method who handles stalk; It is characterized in that said preparing method's step is following: Candida utilis, saccharomycopsis fibuligera, subtilis, Pediococcus pentosaceus, healthy and free from worry Trichoderma kind are inoculated into respectively in the seed culture medium separately, under 28-32 ℃, ventilation condition, cultivated 24-132 hour; Be inoculated into respectively in the fermention medium by the preparation of corn saccharification liquid cultivating sophisticated seed, its inoculum size was 10%, 32 ± 1 ℃, ventilation condition bottom fermentation 24-96 hour; To cultivate sophisticated seed and add wheat bran and carry out freezingly, dry, must handle the probiotic bacterium mixed preparation microbial inoculum product of stalk.
2. a kind of probiotic bacterium mixed preparation preparation method who handles stalk according to claim 1 is characterized in that the mould seed culture medium of described Pediococcus pentosaceus and healthy and free from worry wood is restrained ammonium sulfate and formed by 50 gram straw powder, 10 gram wheat bran and 10; When manufacturing; With 50 gram straw powder, 10 gram wheat bran and 10 gram ammonium sulfate mix mixture, in mixture, add 1000 ml water solution, stir; The mixture aqueous solution is poured into 60 milliliters respectively in the triangular flask of 250 milliliters of volumes; Triangular flask is placed shaking table, under 32 ℃, 120 rev/mins conditions, cultivated 132 hours, obtain seed culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2012101440813A CN102643747A (en) | 2012-05-10 | 2012-05-10 | Method for preparing probiotics mixed preparation for processing straws |
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| CN2012101440813A CN102643747A (en) | 2012-05-10 | 2012-05-10 | Method for preparing probiotics mixed preparation for processing straws |
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| CN102643747A true CN102643747A (en) | 2012-08-22 |
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| CN2012101440813A Pending CN102643747A (en) | 2012-05-10 | 2012-05-10 | Method for preparing probiotics mixed preparation for processing straws |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704501A (en) * | 2013-12-09 | 2014-04-09 | 平顶山中宏华龙饲料有限公司 | Method for producing high-efficiency broiler feed by utilization of corn straw fermentation |
| CN104222488A (en) * | 2013-06-13 | 2014-12-24 | 马放 | Manufacturing method of biological organic high-protein grain-substituted feed conversion agent |
| CN105053743A (en) * | 2015-07-15 | 2015-11-18 | 昆明理工大学 | Silage fermentation accelerator and application thereof |
| CN106282037A (en) * | 2015-12-22 | 2017-01-04 | 贺业欣 | The purebred ventilating fermentation of solid produces feed yeast technology |
| CN110353087A (en) * | 2019-07-11 | 2019-10-22 | 南京农业大学 | The preparation method and fermented feed of rape stalk fermented feed |
Citations (1)
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| CN1552233A (en) * | 2003-05-26 | 2004-12-08 | 哈尔滨中冠生物工程有限公司 | Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation |
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- 2012-05-10 CN CN2012101440813A patent/CN102643747A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1552233A (en) * | 2003-05-26 | 2004-12-08 | 哈尔滨中冠生物工程有限公司 | Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation |
Non-Patent Citations (3)
| Title |
|---|
| 刁彩霞 等: "康宁木霉诱变菌株产酶条件的研究", 《饲料博览》, no. 12, 25 December 2010 (2010-12-25) * |
| 张世敏 等: "秸秆降解菌制剂的研究初报", 《河南农业大学学报》, vol. 35, no. 3, 30 September 2001 (2001-09-30) * |
| 麻名汉 等: "益生菌发酵秸秆的发酵条件研究", 《现代化农业》, no. 11, 15 November 2010 (2010-11-15) * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222488A (en) * | 2013-06-13 | 2014-12-24 | 马放 | Manufacturing method of biological organic high-protein grain-substituted feed conversion agent |
| CN103704501A (en) * | 2013-12-09 | 2014-04-09 | 平顶山中宏华龙饲料有限公司 | Method for producing high-efficiency broiler feed by utilization of corn straw fermentation |
| CN103704501B (en) * | 2013-12-09 | 2015-04-08 | 平顶山中宏华龙饲料有限公司 | Method for producing high-efficiency broiler feed by utilization of corn straw fermentation |
| CN105053743A (en) * | 2015-07-15 | 2015-11-18 | 昆明理工大学 | Silage fermentation accelerator and application thereof |
| CN106282037A (en) * | 2015-12-22 | 2017-01-04 | 贺业欣 | The purebred ventilating fermentation of solid produces feed yeast technology |
| CN110353087A (en) * | 2019-07-11 | 2019-10-22 | 南京农业大学 | The preparation method and fermented feed of rape stalk fermented feed |
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Application publication date: 20120822 |