CN104381607B - A kind of phycomycete composite fermented feed additive and preparation method thereof - Google Patents

A kind of phycomycete composite fermented feed additive and preparation method thereof Download PDF

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CN104381607B
CN104381607B CN201410660199.0A CN201410660199A CN104381607B CN 104381607 B CN104381607 B CN 104381607B CN 201410660199 A CN201410660199 A CN 201410660199A CN 104381607 B CN104381607 B CN 104381607B
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phycomycete
feed additive
fermented
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composite
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CN104381607A (en
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孙利芹
左志鹏
林剑
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Hunan gengtianxia Biotechnology Co., Ltd
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Yantai University
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Abstract

The invention discloses a kind of phycomycete composite fermented feed additive and preparation method thereof;The phycomycete composite fermented feed additive contains the component of following weight portion:10 40 parts of algae slurries, 58 parts of molasses, 58 parts of residue of soyas, 25 35 parts of yeast pastes, 15 30 parts of dregs of beans, 10 30 parts of complex microorganism bacteria suspensions, 0.01 0.12 parts of cellulases, 95 182 parts of water;Said components are prepared by semisolid three-level mixed fermentation technology;The feedstuff additive product that the present invention is compounded by science and microorganism mixed fermentation technology is produced, rich in high-quality single cell protein, active beneficial bacterium and various hydrolytic enzyme activities, while promoting livestock and poultry animal to grow, it is effectively improved animal intestinal tract health, and livestock and poultry animal immunity can be effectively improved, the consumption of antibiotic is reduced, while improving the conversion ratio of feed.

Description

A kind of phycomycete composite fermented feed additive and preparation method thereof
Technical field
The present invention relates to biofermentation and feed additive production field, specifically a kind of phycomycete fermented composite feed Additive and preparation method thereof, i.e., be combined other microbial bacterial agents and fermentation auxiliary material using unicellular alga, carries out semisolid hair Ferment produces additive for farm animal feed.
Background technology
In recent years, use of many countries to antibiotic has been put into effect corresponding limitation or has forbidden policy in the world, and this is caused Setting up in a wide range feed resource, development green ecological livestock and poultry breeding industry turns into the task of top priority of feedstuff industry, therefore, countries in the world are all in product Find and explore new feed resource in pole.Fermented feed refers to that the metabolic activity that microorganism passes through itself will be raised under manually controlling Material raw material in ANFs decompose, synthesis or convert, so as to produce more can by livestock and poultry digest and absorb nutrient and The feed of nonhazardous, its significant effect has caused extensive concern, wherein, using complex micro organism fungicide (EM bacterium) as additive Fermented feed at home and abroad suffers from extremely wide application, and research finds that EM bacterium can improve the conversion ratio of pig feed, reduce abdomen Rush down rate, improve swine product quality, significantly increase the immunologic function of pig, improve resistance against diseases;Tiny ecosystem system is added in daily grain of chicken Agent can improve laying rate, growth performance and the immunity of laying breed.But simple microorganism formulation feed addictive is due to bacterium Complicated, easy inactivation of originating is planted, the direction that various bacterial strains are combined, screen, cultivated is different with method, and fermented feed quality is difficult to Identification, and due to the level of production, animal species and feed manufacturing, storage, the difference for feeding condition element, fermentative feedstuff of microbe Application effect differ greatly.Spirulina and chlorella as unicellular microalgae, not only rich in high-quality single cell protein (in cell Protein content is up to more than 65%), and rich in unrighted acid, vitamin, several mineral materials and pigment etc., security it is good and Have no side effect, have functions that uniqueness in the quality for improving livestock and poultry immunity of organisms, improvement meat and egg.Therefore, unicellular microalgae with Its unique nutritional ingredient and physiological activity, tempting evil spirit is showed in the development field of animal feed and feed addictive Power.But mixed feed is made directly to add algae powder more as the exploitation of feed addictive to spirulina or chlorella at present Form, and it is used for the production of aquatic animal feed, it is used for livestock and poultry breeding industry feed addictive for spirulina and chlorella Production development is very few.Therefore, one kind is developed suitable for livestock and poultry (particularly pig and chicken) cultivation rich in high-quality protein and work Property microbial inoculum feed addictive prescription and production and processing technology, for realize green cultivation and pollution-free food production have very Important meaning.
The content of the invention
A kind of technical problems to be solved by the invention, in view of the problems of prior art, there is provided the compound hair of phycomycete Ferment feed addictive and preparation method thereof, phycomycete composite fermented feed additive of the present invention is by adding spirulina or small Ball algae carries out science compounding with other auxiliary materials and complex micro organism fungicide, carries out three grade fermemtation, can improve animal and fowl fodder addition Various probiotics quantity and hydrolysis activity in agent, increase the content of the nutritional ingredients such as total protein, total fat, improve livestock and poultry animal Especially the utilization rate of the immunity of piglet and feed, reduces the use of antibiotic product.
The technical scheme that the present invention solves above-mentioned technical problem is as follows:A kind of phycomycete composite fermented feed additive, contains The component of following weight portion:10-40 portions of algae slurry, 5-8 parts of molasses, 5-8 parts of residue of soya, 25-35 parts of yeast paste, 15-30 parts of dregs of beans, 10-30 parts of complex microorganism bacteria suspension, 0.01-0.12 parts of cellulase, 95-182 parts of water.
A kind of preparation method for preparing phycomycete composite fermented feed additive, comprises the following steps:
1) by algae powder (spirulina powder or chlorella algae powder) and water according to 1:10 ratio modulation, obtains algae slurry;
2) 10-40 weight portions step 1 is weighed) algae that obtains slurry and 0.01-0.12 part by weight of cellulose enzymes, it is well mixed, Broken wall treatment is carried out, temperature is 40-45 DEG C, and pH value is 5.5, stirs 24h, obtain broken wall treatment raw material algae slurry;
3) 5-8 weight portion residue of soyas are weighed, 20-32 weight portion water is added, is well mixed, obtain dilute residue of soya liquid;
4) by step 3) dilute residue of soya liquid for obtaining and 25-35 weight portion yeast pastes, it is well mixed, fermented and cultured, 28-34 DEG C, 60-100rpm continuously cultivates 24h, obtains mixture I;
5) 15-30 weight portion dregs of beans is weighed, 75-150 weight portion water is added, is well mixed, infiltrated, obtain dilute dregs of beans Liquid;
6) by complex micro organism fungicide according to inoculum concentration 8-10% ratios, cut-in quality fraction is water-soluble for the molasses of 5-8% In liquid, fermented and cultured, 30-34 DEG C, 200-300rpm stirrings are continuously ventilated according to ventilation ratio 0.3-0.5vvm, cultivate 20-24h, Obtain complex microorganism bacteria suspension;
7) by step 5) the dilute dregs of beans liquid, the 10-30 weight portions step 6 that obtain) the complex microorganism bacteria suspension that obtains and 5-8 weight portions molasses are well mixed, fermented and cultured, and 28-34 DEG C of culture, 60-100rpm rotating speed culture 24h obtain mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) obtain it is mixed Compound II is well mixed, fermented and cultured, and temperature is 32-34 DEG C, and 60-100rpm stirrings, fermentation time is 8-10d, and batch (-type) leads to Gas (ventilate 30min per 12h, and ventilation ratio is 0.1-0.3vvm), obtains the phycomycete composite fermented feed additive.
In the step of above-mentioned preparation method, the residue of soya is the residue after the high-salt fermentation moromi squeezing of soy sauce factory;Institute State yeast paste and come from the 6-7 generations discarded saccharomyces cerevisiae mud produced in beer fermentation process, water content is mass fraction 75- 80%;The complex micro organism fungicide, is market finished product purchased from the victory bio tech ltd of Shanghai three, mainly by photosynthetic thin The single bacterial strain compound criteria such as bacterium, lactic acid bacteria, bacillus, Bifidobacterium, saccharomycete is formed.
Phycomycete composite fermented feed additive of the present invention to be taken directly feed method and/or feed method indirectly and is fed Livestock and poultry;Directly feed method, will phycomycete composite fermented feed additive of the present invention according to livestock and poultry diet total amount quality Fraction 10-15% directly feeds simultaneously with other feeds;Method is fed indirectly, will phycomycete fermented composite feed of the present invention Additive shifts to an earlier date and mixes with livestock and poultry diet, and mixer stirs, and is fed after placing 24h, and 2-3d can be used after a spice.
The beneficial effects of the invention are as follows:
1) nutritious, good absorbing effect:Product phycomycete composite fermented feed additive of the present invention with the addition of spirulina or Chlorella, contains abundant nutrition;By taking unicellular microalgae spirulina as an example, " optimal high protein source " good reputation, its egg is have Bai Hanliang is up to the 60-70% of stem cell weight, and amino acid composition is reasonable, containing amino acid necessary to more than 18 kinds animal bodies, Close to the ratio of FAO, and containing various bioactivators such as spirulina polysaccharide, unrighted acids, animal can not only be met Nutritional need, remarkably promote growth of animal, improve product quality and improve the reproductive performance of animal, and body can be strengthened Immunologic function, significantly improves the survival rate of cultivated animals;Current spirulina is mainly carried by specific crowd of functional health care product form For the supplement such as albumen, trophic factors;Spirulina is mainly used in the water such as fish, Shrimp waste, shellfish as the application of feed addictive Animal is produced, application form mainly there are two kinds:A kind of occupation mode is directly to be fed microalgae as food organisms, and another kind is used Mode is then formed into algae and dries algae powder processed, directly as the aquatic livestock mixed feed such as livestock and poultry and fish, shrimp, crab, shellfish, ginseng A kind of raw material or functional feedstuff additive are used;Spirulina and microbial bacterial agent compounding fermentation are used for livestock and poultry by the present invention There is not been reported for the technology of animal feed;The present invention is used after spirulina or chlorella ferment with microbial bacterial agent compounding, more The deficiencies in the prior art are mended, algae is reached the effect of effective broken wall, the molten of frustule active ingredient can have been promoted Go out;On the other hand by the fermentation process of composite bacteria agent, the macromolecular substances in algae can be degraded, is conducive to animal To nutrition and the absorption of functional component;The cellulase that the present invention is used carries out broken wall treatment to algae slurry, is more beneficial in algae slurry The absorption of nutrition;Molasses used in the present invention, are mainly used in the carbon source of microbial bacterial agent growth, while can also improve feeding The palatability of material;Residue of soya used in the present invention, containing various moulds such as aspergillus, after cultivating again aspergillus can secreting amylase, The hydrolases such as protease, while also contain several amino acids in residue of soya, it is nutritious;Yeast paste used in the present invention comes from Waste gas brewer's yeast in beer fermentation process, yeast cells contains high-quality protein in itself, can be simultaneously reached aid digestion, improvement The effect of animal intestinal tract function, increases the utilization rate of feed while waste higher value application.
2) product characteristics are stable, easy to use:Phycomycete composite fermented feed additive of the present invention, it is simple with current Microbial bacterial agent feed addictive is compared, due to the compounding such as algae powder, residue of soya, yeast paste after again by three grade fermemtation process, can To overcome simple microorganism formulation feed addictive because strain source is complicated, easy inactivation, various bacterial strains combinations, screening, training The direction educated is different with method, and fermented feed quality is difficult to identify, and due to the level of production, animal species and feed manufacturing, Storage, the difference of feeding condition element, the shortcoming that the application effect of fermentative feedstuff of microbe differs greatly, products obtained therefrom proterties are steady It is fixed, it is easy to use.
3) phycomycete composite fermented feed additive of the present invention using scientific formula due to mixing hair with microbial bacterial agent Ferment, hydrolyzes that enzyme class is complete, enzymatic activity is high in products obtained therefrom, after being applied as feed addictive, can effectively improve livestock and poultry feeding Material conversion ratio, improves livestock and poultry animal intestinal health.
4) residue of soya in the formulation auxiliaries that phycomycete composite fermented feed additive of the present invention is used, yeast paste all belong to It is with low cost in plant produced workshop leftover bits and pieces, after the technology of the present invention is re-used, it is possible to achieve waste material Change the purpose that increment is utilized, for utilizing for factory's leftover bits and pieces provides a new approach;Simultaneously by fermentation of the present invention Technology, it is possible to use in residue of soya the characteristics of the growth metabolism process secretion hydrolase of mould and yeast, increase hydrolase in feed Activity, there is provided the conversion ratio of animal and fowl fodder.
Brief description of the drawings
Fig. 1 is the preparation method procedure chart that the present invention prepares phycomycete composite fermented feed additive.
Specific embodiment
A kind of phycomycete composite fermented feed additive, the component containing following weight portion:10-40 portions of algae slurry, 5-8 portions of sugar Honey, 5-8 parts of residue of soya, 25-35 parts of yeast paste, 15-30 parts of dregs of beans, 10-30 parts of complex microorganism bacteria suspension, 0.01-0.12 parts of fibre The plain enzyme of dimension, 95-182 parts of water.
A kind of preparation method for preparing phycomycete composite fermented feed additive, comprises the following steps:
1) by algae powder (spirulina powder or chlorella algae powder) and water according to 1:10 ratio modulation, obtains algae slurry;
2) 10-40 weight portions step 1 is weighed) algae that obtains slurry and 0.01-0.12 part by weight of cellulose enzymes, it is well mixed, Broken wall treatment is carried out, temperature is 40-45 DEG C, and pH value is 5.5, stirs 24h, obtain broken wall treatment raw material algae slurry;
3) 5-8 weight portion residue of soyas are weighed, 20-32 weight portion water is added, is well mixed, obtain dilute residue of soya liquid;
4) by step 3) dilute residue of soya liquid for obtaining and 25-35 weight portion yeast pastes, it is well mixed, fermented and cultured, 28-34 DEG C, 60-100rpm continuously cultivates 24h, obtains mixture I;
5) 15-30 weight portion dregs of beans is weighed, 75-150 weight portion water is added, is well mixed, obtain dilute dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 8-10% ratios, cut-in quality fraction is water-soluble for the molasses of 5-8% In liquid, fermented and cultured, 30-34 DEG C, 200-300rpm stirrings are continuously ventilated according to ventilation ratio 0.3-0.5vvm, cultivate 20-24h, Obtain complex microorganism bacteria suspension;
7) by step 5) the dilute dregs of beans liquid, the 10-30 weight portions step 6 that obtain) the complex microorganism bacteria suspension that obtains and 5-8 weight portions molasses are well mixed, fermented and cultured, and 28-34 DEG C of culture, 60-100rpm rotating speed culture 24h obtain mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) obtain it is mixed Compound II is well mixed, fermented and cultured, and temperature is 32-34 DEG C, and 60-100rpm stirrings, fermentation time is 8-10d, and batch (-type) leads to Gas (ventilate 30min per 12h, and ventilation ratio is 0.1-0.3vvm), obtains the phycomycete composite fermented feed additive.
Accompanying drawing 1 indicates the process of the preparation method of above-mentioned phycomycete composite fermented feed additive.
Above-described weight portion with it is kilogram corresponding in embodiment.
Embodiment 1
A kind of phycomycete composite fermented feed additive, contains:10kg algaes slurry, 5kg molasses, 5kg residue of soyas, 25kg yeast pastes, 25kg dregs of beans, 10kg complex microorganisms bacteria suspension, 0.01kg cellulases, 145kg water.
A kind of preparation method for preparing phycomycete composite fermented feed additive, comprises the following steps:
1) by spirulina powder and water according to 1:10 ratio modulation, obtains algae slurry;
2) 10kg steps 1 are weighed) algae that obtains slurry and 0.01kg cellulases, it is well mixed, carry out broken wall treatment, temperature It is 40 DEG C, pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae slurry;
3) 5kg residue of soyas are weighed, 20kg water is added, is well mixed, obtain dilute residue of soya liquid;
4) by step 3) dilute residue of soya liquid for obtaining and 25kg yeast pastes, it is well mixed, fermented and cultured, 28 DEG C, 60rpm, even Continuous culture 24h, obtains mixture I;
5) 25kg dregs of beans is weighed, 125kg water is added, is well mixed, obtain dilute dregs of beans liquid;
6) by complex micro organism fungicide according to the ratio of inoculum concentration 8%, during cut-in quality fraction is the 5% molasses aqueous solution, Fermented and cultured, 30 DEG C, 200rpm stirrings are continuously ventilated according to ventilation ratio 0.3vvm, cultivate 20h, are obtained composite microbial bacteria and are hanged Liquid;
7) by step 5) the dilute dregs of beans liquid, the 10kg steps 6 that obtain) the complex microorganism bacteria suspension and 5kg molasses that obtain Well mixed, fermented and cultured, 28 DEG C of cultures, 60rpm rotating speed culture 24h obtain mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) obtain it is mixed Compound II is well mixed, fermented and cultured, and temperature is 32 DEG C, 60rpm stirrings, and fermentation time is 8d, and batch (-type) ventilation (is led to per 12h Gas 30min, ventilation ratio is 0.1vvm), obtain the phycomycete composite fermented feed additive.
Embodiment 2
A kind of phycomycete composite fermented feed additive, contains:15kg algaes slurry, 8kg molasses, 8kg residue of soyas, 35kg yeast pastes, 30kg dregs of beans, 15kg complex microorganisms bacteria suspension, 0.045kg cellulases, 182kg water.
A kind of preparation method for preparing phycomycete composite fermented feed additive, comprises the following steps:
1) by chlorella algae powder and water according to 1:10 ratio modulation, obtains algae slurry;
2) 15kg steps 1 are weighed) algae that obtains slurry and 0.045kg cellulases, it is well mixed, broken wall treatment is carried out, temperature It is 45 DEG C to spend, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae slurry;
3) 8kg residue of soyas are weighed, 32kg water is added, is well mixed, obtain dilute residue of soya liquid;
4) by step 3) dilute residue of soya liquid for obtaining and 35kg yeast pastes, it is well mixed, fermented and cultured, 34 DEG C, 100rpm, even Continuous culture 24h, obtains mixture I;
5) 30kg dregs of beans is weighed, 150kg water is added, is well mixed, obtain dilute dregs of beans liquid;
6) by complex micro organism fungicide according to the ratio of inoculum concentration 10%, during cut-in quality fraction is the 8% molasses aqueous solution, Fermented and cultured, 34 DEG C, 300rpm stirrings are continuously ventilated according to ventilation ratio 0.5vvm, cultivate 24h, are obtained composite microbial bacteria and are hanged Liquid;
7) by step 5) the dilute dregs of beans liquid, the 15kg steps 6 that obtain) the complex microorganism bacteria suspension and 8kg molasses that obtain Well mixed, fermented and cultured, 34 DEG C of cultures, 100rpm rotating speed culture 24h obtain mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) obtain it is mixed Compound II is well mixed, fermented and cultured, and temperature is 34 DEG C, 100rpm stirrings, and fermentation time is 10d, and batch (-type) ventilation is (per 12h Ventilation 30min, ventilation ratio is 0.3vvm), obtain the phycomycete composite fermented feed additive.
Embodiment 3
A kind of phycomycete composite fermented feed additive, contains:12kg algaes slurry, 7kg molasses, 7kg residue of soyas, 30kg yeast pastes, 27kg dregs of beans, 12kg complex microorganisms bacteria suspension, 0.024kg cellulases, 163kg water.
A kind of preparation method for preparing phycomycete composite fermented feed additive, comprises the following steps:
1) by spirulina powder and water according to 1:10 ratio modulation, obtains algae slurry;
2) 12kg steps 1 are weighed) algae that obtains slurry and 0.024kg cellulases, it is well mixed, broken wall treatment is carried out, temperature It is 42 DEG C to spend, and pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae slurry;
3) 7kg residue of soyas are weighed, 28kg water is added, is well mixed, obtain dilute residue of soya liquid;
4) by step 3) dilute residue of soya liquid for obtaining and 30kg yeast pastes, it is well mixed, fermented and cultured, 30 DEG C, 80rpm, even Continuous culture 24h, obtains mixture I;
5) 27kg dregs of beans is weighed, 135kg water is added, is well mixed, obtain dilute dregs of beans liquid;
6) by complex micro organism fungicide according to the ratio of inoculum concentration 9%, during cut-in quality fraction is the 7% molasses aqueous solution, Fermented and cultured, 32 DEG C, 250rpm stirrings are continuously ventilated according to ventilation ratio 0.4vvm, cultivate 22h, are obtained composite microbial bacteria and are hanged Liquid;
7) by step 5) the dilute dregs of beans liquid, the 12kg steps 6 that obtain) the complex microorganism bacteria suspension and 57kg molasses that obtain Well mixed, fermented and cultured, 30 DEG C of cultures, 80rpm rotating speed culture 24h obtain mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) obtain it is mixed Compound II is well mixed, fermented and cultured, and temperature is 33 DEG C, 80rpm stirrings, and fermentation time is 9d, and batch (-type) ventilation (is led to per 12h Gas 30min, ventilation ratio is 0.2vvm), obtain the phycomycete composite fermented feed additive.
Embodiment 4
A kind of phycomycete composite fermented feed additive, contains:40kg algaes slurry, 5kg molasses, 5kg residue of soyas, 25kg yeast pastes, 15kg dregs of beans, 30kg complex microorganisms bacteria suspension, 0.12kg cellulases, 95kg water.
A kind of preparation method for preparing phycomycete composite fermented feed additive, comprises the following steps:
1) by spirulina powder and water according to 1:10 ratio modulation, obtains algae slurry;
2) 40kg steps 1 are weighed) algae that obtains slurry and 0.12kg cellulases, it is well mixed, carry out broken wall treatment, temperature It is 40 DEG C, pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae slurry;
3) 5kg residue of soyas are weighed, 20kg water is added, is well mixed, obtain dilute residue of soya liquid;
4) by step 3) dilute residue of soya liquid for obtaining and 25kg yeast pastes, it is well mixed, fermented and cultured, 28 DEG C, 60rpm, even Continuous culture 24h, obtains mixture I;
5) 15kg dregs of beans is weighed, 75kg water is added, is well mixed, obtain dilute dregs of beans liquid;
6) by complex micro organism fungicide according to the ratio of inoculum concentration 8%, during cut-in quality fraction is the 5% molasses aqueous solution, Fermented and cultured, 30 DEG C, 200rpm stirrings are continuously ventilated according to ventilation ratio 0.3vvm, cultivate 20h, are obtained composite microbial bacteria and are hanged Liquid;
7) by step 5) the dilute dregs of beans liquid, the 30kg steps 6 that obtain) the complex microorganism bacteria suspension and 5kg molasses that obtain Well mixed, fermented and cultured, 28 DEG C of cultures, 60rpm rotating speed culture 24h obtain mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) obtain it is mixed Compound II is well mixed, fermented and cultured, and temperature is 32 DEG C, 60rpm stirrings, and fermentation time is 8d, and batch (-type) ventilation (is led to per 12h Gas 30min, ventilation ratio is 0.1vvm), obtain the phycomycete composite fermented feed additive.
Embodiment 5
A kind of phycomycete composite fermented feed additive, contains:35kg algaes slurry, 5kg molasses, 5kg residue of soyas, 25kg yeast pastes, 15kg dregs of beans, 25kg complex microorganisms bacteria suspension, 0.07kg cellulases, 95kg water.
A kind of preparation method for preparing phycomycete composite fermented feed additive, comprises the following steps:
1) by chlorella powder and water according to 1:10 ratio modulation, obtains algae slurry;
2) 35kg steps 1 are weighed) algae that obtains slurry and 0.01kg cellulases, it is well mixed, carry out broken wall treatment, temperature It is 40 DEG C, pH value is 5.5, stirs 24h, obtains broken wall treatment raw material algae slurry;
3) 5kg residue of soyas are weighed, 20kg water is added, is well mixed, obtain dilute residue of soya liquid;
4) by step 3) dilute residue of soya liquid for obtaining and 25kg yeast pastes, it is well mixed, fermented and cultured, 28 DEG C, 60rpm, even Continuous culture 24h, obtains mixture I;
5) 15kg dregs of beans is weighed, 75kg water is added, is well mixed, obtain dilute dregs of beans liquid;
6) by complex micro organism fungicide according to the ratio of inoculum concentration 8%, during cut-in quality fraction is the 5% molasses aqueous solution, Fermented and cultured, 30 DEG C, 200rpm stirrings are continuously ventilated according to ventilation ratio 0.3vvm, cultivate 20h, are obtained composite microbial bacteria and are hanged Liquid;
7) by step 5) the dilute dregs of beans liquid, the 25kg steps 6 that obtain) the complex microorganism bacteria suspension and 5kg molasses that obtain Well mixed, fermented and cultured, 28 DEG C of cultures, 60rpm rotating speed culture 24h obtain mixture II;
8) by step 2) the broken wall treatment raw material algae that obtains slurry, step 4) mixture I that obtains and step 7) obtain it is mixed Compound II is well mixed, fermented and cultured, and temperature is 32 DEG C, 60rpm stirrings, and fermentation time is 8d, and batch (-type) ventilation (is led to per 12h Gas 30min, ventilation ratio is 0.1vvm), obtain the phycomycete composite fermented feed additive.
The product feature of the phycomycete composite fermented feed additive of the present invention of embodiment 6
Phycomycete composite feed additive prepared by the present invention is light brown, there is the semi-solid products of algae powder fragranced, moisture Content is mass fraction 55-60%, pH 5.5-6.0.Beneficial bacterium sum (cfu/mL) is about 1.6 × l0 in product10, wherein ferment Female 8.9 × l0 of bacterium7, 6.8 × l0 of lactic acid bacteria9, 8.4 × l0 of photosynthetic bacteria9, 7.4 × l0 of Bifidobacterium6, 4.5 × l0 of bacillus6; The species of the hydrolase detected in product is respectively with enzyme activity unit (U/mL):Cellulase 30.2, AMS 1926.6, beta amylase 702, acid protease 402.52, neutral proteinase 147.44, alkali protease 100.36;In product Total protein content (mass ratio) is 32.1%, total fat 5.7%, coarse ash 8.2%, phosphorus 0.3%, calcium 0.56%.
The phycomycete composite feed additive product composition of table 1 is analyzed
It can be seen from the data of phycomycete composite feed additive product composition analysis in table 1, phycomycete composite feed additive The content of the nutriment such as total protein, total fat, phosphorus, calcium is also excellent considerably beyond the minimum of national standard requirement in product In other many fermented feeds.For example, crude protein content is 26.13% in the composite probiotic fermented feed of the research such as Li Long; Protein content is 15.72% in the fermentation straw feed of the researchs such as Bai Xiaojun;The multiple bacteria compound fermentation of the researchs such as Liao Xueyi Protein content is 24.61% during stalk produces protein feeds.
Enzyme activity unit in the phycomycete composite feed additive finished product of table 2
The scholar such as strong is appointed to examine the enzyme activity in Tianjin Xiang Hua biological feedstuffs Co., Ltd CHE Feed Samples Survey, discovery alpha-amylase activity is 5.6352 μ g/ (gmin), beta amylase vigor is 1.0931 μ g/ (gmin), acid egg White enzyme activity is 5.203 μ g/ (gmin), and neutral protease vigor is 2.9754 μ g/ (gmin), is compared after unit conversion Compared with the enzyme activity that enzyme activity is determined in reaching and being studied more than even above in this phycomycete composite feed additive product is high Enzymatic activity has important meaning for improving the conversion ratio of feed, promotion body to the overall absorption of nutritional ingredient.
The method reference national standard detection method of the said goods index detection method, such as on the analysis side of product composition Method reference:Total protein content determine according to standard GB/T 5009.5-2010, total lipid content determine according to standard GB/T/ T22223-2008, coarse ash determine according to standard GB/T/T6438-2007, phosphorus detection according to standard GB/T/ T6437-2002, determination of calcium content are according to standard GB/T/T 6436-2002.
Enzyme-activity unit is defined as follows in the hydrolysis activity detection method of phycomycete composite feed additive prepared by the present invention:
Cellulase activity unit definition:At 50 DEG C, enzyme needed for 1 μ g glucose of hydrolysis generation per minute under corresponding pH value Amount be defined as a unit of activity.Assay method uses CMC methods.
Diastatic activity unit definition:At 40 DEG C, hydrolysis starch per minute produces 1 μ g maltose sugar required under corresponding pH value Enzyme amount be defined as 1 unit of enzyme activity.Assay method is according to standard GB/T 8275-2009.
Protease activity unit definition:At 40 DEG C, under corresponding pH value needed for 1 μ g tyrosine of hydrolyzed casein generation per minute Enzyme amount is defined as 1 unit of enzyme activity.Assay method uses Folin reagent reducing process.
Embodiment 7
Phycomycete composite feed additive of the present invention is fed into piglet, specific feeding method is:Raised in weanling pig The phycomycete composite feed additive of the present invention that mass fraction is 15% is added in material, is fed 5 times within 1st, fed containing adding every time Plus 0.2 kilogram of the feed of agent;The feed without additive is set as a control group simultaneously.Test result indicate that:In weanling pig The phycomycete composite feed additive of the present invention of mass fraction 15% is added in feed, can be improved a poor appetite, effectively improved The speed of growth of piglet, daily gain is about 10-15%, while efficiency of feed utilization improves 6-8%, feed intake improves 3-4%, while The generation of diarrhea of weaned piglets is reduced, strengthens body's resistance to disease, continuously use the piglet after 50 days, compared with control group, hair Shinny, vigor is more prosperous.
Embodiment 8
Phycomycete composite feed additive of the present invention is fed into Broiler chicks (1-3 week old), specific feeding method is: It is the phycomycete composite feed additive of the present invention of 10-15% that mass fraction is added in Broiler chicks basal diet, is fed within 1st 5-6 times, it is 150-300g that the feed total amount containing additive is fed daily;Basal diet group conduct without additive is set simultaneously Control.Test result indicate that:Mass fraction 10-15% phycomycete mixed feed additions of the present invention are added in broiler diet Agent, makes chick daily gain improve 4.8-6.5%, and survivability of chicks improves 5.5-6.7%, and the immunity of organisms of chick is improved, energy It is obviously promoted to grow.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all it is of the invention spirit and Within principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.

Claims (8)

1. a kind of preparation method of phycomycete composite fermented feed additive, it is characterised in that comprise the following steps:
1) by algae powder and water according to 1:10 ratio modulation, obtains algae slurry;The algae powder is spirulina powder or chlorella algae powder;
2) 10-40 weight portions step 1 is weighed) algae that obtains slurry and 0.01-0.12 part by weight of cellulose enzymes, it is well mixed, carry out Broken wall treatment, obtains broken wall treatment raw material algae slurry;
3) 5-8 weight portion residue of soyas are weighed, 20-32 weight portion water is added, is well mixed, obtain dilute residue of soya liquid;
4) by step 3) dilute residue of soya liquid for obtaining and 25-35 weight portion yeast pastes, it is well mixed, fermented and cultured obtains mixture Ⅰ;
5) 15-30 weight portion dregs of beans is weighed, 75-150 weight portion water is added, is well mixed, infiltrated, obtain dilute dregs of beans liquid;
6) by complex micro organism fungicide according to inoculum concentration 8-10% ratios, during cut-in quality fraction is for the molasses aqueous solution of 5-8%, Fermented and cultured, obtains complex microorganism bacteria suspension;
7) by step 5) the dilute dregs of beans liquid, the 10-30 weight portions step 6 that obtain) the complex microorganism bacteria suspension and 5-8 weights that obtain Amount part molasses are well mixed, and fermented and cultured obtains mixture II;
8) by step 2) raw material algae slurry, step 4 after the broken wall treatment that obtains) mixture I that obtains and step 7) and obtain it is mixed Compound II is well mixed, and fermented and cultured obtains the phycomycete composite fermented feed additive.
2. the preparation method of phycomycete composite fermented feed additive according to claim 1, it is characterised in that step 2) in, The condition of the broken wall treatment is:Temperature is 40-45 DEG C, and pH value is 5.5, stirs 24h.
3. the preparation method of phycomycete composite fermented feed additive according to claim 1, it is characterised in that step 4) in, The condition of the fermented and cultured is 28-34 DEG C, and 60-100rpm continuously cultivates 24h.
4. the preparation method of phycomycete composite fermented feed additive according to claim 1, it is characterised in that step 6) in, The condition of the fermented and cultured is:30-34 DEG C, 200-300rpm stirrings are continuously ventilated according to ventilation ratio 0.3-0.5vvm, are cultivated 20-24h。
5. the preparation method of phycomycete composite fermented feed additive according to claim 1, it is characterised in that step 7) in, The condition of the fermented and cultured is:28-34 DEG C of culture, 60-100rpm rotating speed cultures 24h.
6. the preparation method of phycomycete composite fermented feed additive according to claim 1, it is characterised in that step 8) in, The condition of the fermented and cultured is:Temperature is 32-34 DEG C, 60-100rpm stirrings, and fermentation time is 8-10d, batch (-type) ventilation hair Ferment.
7. the preparation method of phycomycete composite fermented feed additive according to claim 6, it is characterised in that the batch (-type) The aeration condition of aerobic fementation is:Ventilate 30min per 12h, and ventilation ratio is 0.1-0.3vvm.
8. the phycomycete composite fermented feed additive that prepared by a kind of preparation method as described in claim any one of 1-7 is in aquaculture Application.
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