CN103859237B - A kind of compound aquatic product micro-capsule open-mouthed bait and preparation method thereof and application - Google Patents
A kind of compound aquatic product micro-capsule open-mouthed bait and preparation method thereof and application Download PDFInfo
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- CN103859237B CN103859237B CN201410132158.4A CN201410132158A CN103859237B CN 103859237 B CN103859237 B CN 103859237B CN 201410132158 A CN201410132158 A CN 201410132158A CN 103859237 B CN103859237 B CN 103859237B
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- 239000003094 microcapsule Substances 0.000 title claims abstract description 36
- 208000032963 Capsule physical issue Diseases 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 150000001875 compounds Chemical class 0.000 title claims abstract description 18
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 46
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 239000004310 lactic acid Substances 0.000 claims abstract description 23
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 22
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- 229920001184 polypeptide Polymers 0.000 claims abstract description 20
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 17
- 239000000203 mixture Substances 0.000 claims abstract description 12
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 11
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Abstract
The invention discloses a kind of compound aquatic product micro-capsule open-mouthed bait and its preparation method and application, this open-mouthed bait pressurizes by be primary raw material containing different proportion fly-maggot protein polypeptide, edible fungus fermented liquid, lactic acid bacteria, spirulina powder, soybean lecithin, vegetable oil with sodium alginate mix by a certain percentage and sprays into CaCl after high-speed homogenization
2microcapsule granule is formed and the aquatic micro-capsule open-mouthed bait made after filtration in solution.Open-mouthed bait of the present invention has micron and can suspend in water within the regular hour again to millimeter different-grain diameter, be applicable to aquatic seedling the eating at Different growth phases of just hatching, immunity of organisms can be strengthened, the regulating intestinal canal ecological balance promotes that it grows, do not add antibiotic and shelling element, and remain without chemicals, meet the requirement of green bait and feed addictive, preparation cost is cheap, easy to use, extensively can promote the use of at aquatic fry growing and aquaculture field.
Description
Technical field
The present invention relates to a kind of aquaculture microcapsule, particularly the micro-capsule open-mouthed bait and preparation method thereof of aquatic seedling and application.
Background technology
Because the aquatic livestock first time opening of hatching seedling is taken food passive type often, its death of seedling rate is very high.Reason is the high-quality starter diet that natural environment can not provide enough, and breeding water body is due to long-term cyclic culture, have accumulated a large amount of pathogenic microorganism, is unfavorable for growing of aquatic seedling.Therefore breed the key that seedling is artificial feeding aquatic livestock in enormous quantities, the development of open-mouthed bait then becomes key problem in technology.The balanced nutritious composition that will be made up of high animal protein to the basic demand of aquatic seedling open-mouthed bait, so that just hatching is juvenile fish seedling eating and the growth of fish.Simultaneously because fry mouth only has 200 microns of sizes, therefore the particle diameter of this type of open-mouthed bait is less than 200 microns usually, and disperses in Yi Shui.So that seedling swallows.
The aquatic product sprout of China is produced, and mainly adopts fish meal, Micro Algae, strengthening zooplankter for a long time, adds the methods such as meridic diet (yolk, soya-bean milk), solves the open-mouthed bait of the seed young and the bait needed for larval growth cultivation.Along with the development of large-scale cultivation; require to improve to aquaculture Seedling production; the biologically active bait that tradition uses; particularly slag oxygenation; due to the impact by natural environment, produce unstable, not only in output, Seedling production demand can not be met far away; simultaneously due to the price of its costliness, be more the increase in fluctuation and the risk of Seedling production.
China's culture fishery is on a grand scale, but there is many medication problems, causes the residual and accumulation of antibiotic, heavy metal etc. in fish body and water body, the serious development that have impact on China's Non-environmental Pollution Aquiculture.Have over half for herding, culture fishery in domestic use 180,000 tons of antibiotic, aquaculture uses antibiotic amount considerably beyond the summation of the upper use amount of Human clinical.In food, animal, antibiotic residue and these drug-fast bacterias consequent pass through the contact transmission of food or animals and human beings to the mankind, and harm humans is healthy.Export of Chinese Aquatic Products is return the economic loss that causes more than tens yuan because antibiotic exceeds standard, and residues of pesticides and antibiotic exceed standard becomes a bottleneck of restriction Export of Chinese Aquatic Products.
Therefore, find that a kind of dietary protein origin is abundant, quality good, the novel green feed addictive that simultaneously can reduce or replace antibiotic usage has very large Practical significance.
The nutritional labeling of fly-maggot protein is comprehensive, and crude protein content is high, and content reaches 60%, and essential amino acid enriches, and lysine and methionine content are respectively 2.6 and 2.7 times of fish meal.Fly-maggot protein contains a kind of " antibacterial active peptide " of very strong bactericidal action, effectively can suppress the germ of invading, and nontoxic to animals and humans, and meeting the requirement of green cultivation, will be the best bait of aquatic products.But the poorly water-soluble of fly-maggot protein, dissolution rate is low, effectively can not utilize for aquatic seedling.In addition in usual Flyblow albumen powder, the content of antibacterial active peptide is also very low, limits it and further utilizes.
Through amino acid, vitamin, trace element that edible fungus fermented liquid and the mycelium thereof of Submerged liquid culturation enrich, more containing more β-D(1.3) panimmunity such as glucan, xylan, pentosan, GABA, glutathione regulates active material, the physique of aquatic seedling can be strengthened, strengthen its resistance against diseases, reduce or do not use the materials such as antibiotic.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of compound aquatic product micro-capsule open-mouthed bait, can simulate natural aquatic product cultivation open-mouthed bait.
The technical problem that the present invention also will solve is to provide the preparation method of above-mentioned compound aquatic product micro-capsule open-mouthed bait.
The technical problem that the present invention finally will solve is to provide the application of above-mentioned compound aquatic product micro-capsule open-mouthed bait.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of compound aquatic product micro-capsule open-mouthed bait, it comprises the component of following percetage by weight:
Above weight percentages of components sum is 100%;
Additionally add:
Sodium alginate 1 ~ 3%.
A kind of compound aquatic product micro-capsule open-mouthed bait, it preferably includes the component of following percetage by weight:
Above weight percentages of components sum is 100%;
Additionally add:
Sodium alginate 2 ~ 3%.
Wherein, described fly-maggot protein polypeptide liquid, protein concentration is wherein more than 10mg/mL, and amino nitrogen content is more than 10mg/100mL, is less than 1mg/mL to the bacteriostatic activity of Escherichia coli and salmonella.
Wherein, described fly-maggot protein polypeptide liquid prepares as follows: after being mixed with phosphate buffer by fly maggot polypide, high-speed homogenization is smashed, get supernatant after centrifugal to preserve, precipitation continues to mix with phosphate buffer, with phosphoric acid or acetic acid adjust pH to 1 ~ 4, add acid protease in 37 DEG C of enzymolysis 1 ~ 3 hour, then use NaOH adjust pH to 7 ~ 10, again 37 DEG C of enzymolysis 1 ~ 3 hour, enzymolysis liquid mixes with described supernatant and get final product.
Described fly maggot preferably adopts pig, ox or chicken manure incubation hatching housefly maggot, utilize pig, ox or chicken manure microorganism particularly some pathogens such as Escherichia coli, salmonella stimulate the secretion of induction fly maggot to produce various antibacterial peptide or antibacterial substance.
Described fly-maggot protein polypeptide liquid preferably prepares as follows: will by pig, ox or chicken manure incubation hatching housefly maggot are with after clear water rinsed clean, to add in the phosphate buffer of 0.05M after high-speed homogenization machine beats under 6000rpm condition centrifugal 20 minutes by weight the ratio of 2:1, get supernatant to preserve, the centrifugation phosphate buffer of same ratio mixes, pH1 ~ 4 are adjusted to phosphoric acid or acetic acid, add acid protease in 37 DEG C of insulations 1 ~ 3 hour, pH7 ~ 10 are adjusted to again with NaOH, equally 37 DEG C of insulations 1 ~ 3 hour, after enzymolysis, mixture mixes with aforementioned centrifuged supernatant and is high protein concentration, there is the fly-maggot protein polypeptide of certain antibacterial activity.
Wherein, described edible mushroom homogenate, wherein the content of β-1,3 glucan is more than 0.1wt%, and the respective content of wood sugar, xylan and pentosan is more than 1wt%, and alpha-aminobutyric acid content is more than 0.1wt%, and glutathione content is 10 more than μ g/mL.
Wherein, described edible mushroom homogenate prepares as follows: edible mushroom seed liquor added in culture medium by the inoculum concentration of 10 ~ 30wt% and carry out liquid deep layer fermenting and cultivate and zymotic fluid and mycelium thereof to be beaten through refiner after 3 ~ 7 days and get final product, control throughput 5 ~ 35L/ hour during fermented and cultured, dissolved oxygen amount controls in DO value 10 ~ 30%; Wherein, described culture medium comprises the component of following percetage by weight: brewex's grains 5 ~ 30%, wheat bran 1 ~ 5%, analysis for soybean powder 0.05 ~ 1.0%, coarse rice powder 1 ~ 9%, sucrose 1 ~ 3%, dipotassium hydrogen phosphate 0.05 ~ 0.5%, magnesium sulfate 0.03 ~ 0.3%, CaCl
20.1 ~ 3.0%, Cobastab
110 ~ 50mg/L, all the other are water, pH6 ~ 8.
Wherein, any one or a few the mixture that described edible mushroom is mushroom, straw mushroom, mushroom, auricularia auriculajudae, white fungus, hedgehog hydnum, dictyophora phalloidea, Trichotoma matsutake, dried mushroom, russule, bolete, hickory chick, saddle fungus, ferfas and picks up the ears in mushroom.
Wherein, in described lactic acid bacteria bacteria suspension, the content of lactic acid bacteria is 10
6~ 10
12cFU/mL.
Wherein, described lactic acid bacteria bacteria suspension prepares as follows: utilize containing wood sugar, xylan, pentosan zymotic fluid (optional with above-mentioned edible fungus fermented after supernatant be zymotic fluid), fermentation process ferments routinely, and after requiring fermentation ends, content of lactic acid bacteria must not lower than 10
9under CFU/mL, fermentation liquor 3000 ~ 7000rpm condition after centrifugal 5 ~ 30 minutes, the centrifugal supernatant under centrifugal thalline similarity condition is adjusted to 10
6~ 10
12the mixed liquor of CFU/mL, or OD600 value more than 0.1, this is lactic acid bacteria bacteria suspension.Lactic acid bacteria used is the mixed in equal amounts bacterium of streptococcus thermophilus, lactobacillus bulgaricus or two kinds of bacterium.
Compound aquatic product micro-capsule open-mouthed bait of the present invention, contained nutritional labeling is fly-maggot protein polypeptide, edible fungus fermented liquid, lactic acid bacteria, spirulina powder, soybean lecithin, plant wet goods, wherein fly-maggot protein polypeptide has several functions polypeptide (comprising antibacterial peptide) and abundant aliphatic acid, also have 18 seed amino acids and the mineral matter such as calcium, phosphorus, edible fungus fermented liquid, spirulina powder can provide the abundant material such as nucleotides, vitamin, trace element, are the high-quality nutrient sources of aquatic seedling.Fly-maggot protein polypeptide, edible mushroom, streptococcus acidi lactici fermented solution contain more immune polysaccharide, xylan, pentosan, antibacterial peptide, the special active ingredient of GABA class, be conducive to the immunologic function improving aquatic seedling, maintain alimentary canal normal physiological context and the ecological balance, improve resistance against diseases.Soybean lecithin, plant wet goods contain multiple unrighted acid and phospholipid substance, can promote the nervous system of aquatic seedling and even the growth of individuality.By the invention provides various combination of active principles, for aquatic seedling provides high-quality high nutritional labeling growing of different phase, not containing antibiotic and shelling element, meets environment protection requirement, the bait that preparation cost is cheap, easy to use, extensively can promote the use of at green aquatic fry growing and aquaculture field.
The preparation method of above-mentioned compound aquatic product micro-capsule open-mouthed bait, the fly-maggot protein polypeptide liquid of formula ratio, edible mushroom homogenate, lactic acid bacteria bacteria suspension, spirulina powder, soybean lecithin, water and sodium alginate are mixed, the mixing of 40 ~ 60 DEG C of stirring in water bath, then adds after the peanut oil of formula ratio or soya-bean oil high-speed homogenization machine beat and at once sprays into 15 ~ 20wt%CaCl with atomizer pressurization
2form microcapsule granule in the aqueous solution and be less than 1 millimeter of micro-capsule with making particle diameter after 80 mesh sieve filtrations.
Wherein, described peanut oil or the formula ratio of soya-bean oil select more than 2 different amounts, prepare the bait of more than 2 batches, utilize grease proportion lower than the characteristic of water, and utilize the fat content of variable concentrations that the proportion of bait can be made different thus be suspended in the different depth of water.This open-mouthed bait microcapsule granule can in water different depth stable suspersion, can effectively prevent from, in nutriment loss water, substantially increasing its effective availability.
Above-mentioned compound aquatic product micro-capsule open-mouthed bait is preparing the application in aquatic seedling open-mouthed bait.Wherein, described aquatic seedling is preferably prawn seedling.
The bait prepared through the present invention is at once thrown after can preparing at the scene to spread in pool to feed and is raised, also can be stored in and preserve and use for a long time after Co60 irradiates after airtight packing in the fresh-keeping aqueous solution of 15wt% calcium chloride, 10wt% calcium propionate composition, also can grind packing after sieving after drying and preserve.
Beneficial effect: the present invention will to be primary raw material with sodium alginate mix by a certain percentage pressurizes after high-speed homogenization and spray into CaCl containing different proportion fly-maggot protein polypeptide, edible fungus fermented liquid, lactic acid bacteria, spirulina powder, soybean lecithin, vegetable oil
2form microcapsule granule in solution and the micro-capsule made after filtering, for aquatic seedling provide high animal protein, nutritional labeling balanced, there is certain antibacterial ability, the nervous system promoting aquatic seedling and even the growth of individuality, adjustable seedling alimentary canal normal physiological context and ecological balance open-mouthed bait.Raw material of the present invention is All Pure Nature raw material, to aquatic livestock and human non-toxic harmless, environmentally friendly, meet the requirement of green cultivation, thus there are wide market prospects.
Detailed description of the invention
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1:
The preparation of 1.1 fly-maggot protein polypeptide liquid:
Will by pig, ox or chicken manure incubation hatching housefly maggot 1kg are with after clear water rinsed clean, add the phosphate buffer 500mL of 0.05M, after beating with high-speed homogenization machine under 6000rpm condition centrifugal 20 minutes, get supernatant to preserve, centrifugation adds the mixing of same volume 0.05M phosphate buffer, pH3 is adjusted to phosphoric acid, add acid protease in 37 DEG C insulation 1 ~ hour, pH8 is adjusted to again with NaOH, equally 37 DEG C of insulations 1 hour, enzymolysis product mixes with aforementioned centrifuged supernatant, the phosphate buffer adjustment protein concentration adding 0.05M is not less than 10mg/mL, amino nitrogen content is not less than 10mg/100mL, obtain fly-maggot protein polypeptide solution, it is less than 1mg/mL to the bacteriostatic activity of Escherichia coli and salmonella after testing.The preparation of 1.2 edible fungus fermented homogenates:
Get brewex's grains 20wt%, wheat bran 4wt%, analysis for soybean powder 0.6wt%, coarse rice powder 5wt%, sucrose 2wt%, dipotassium hydrogen phosphate 0.2wt%, magnesium sulfate 0.15wt%, CaCl
21.5wt%, Cobastab
120mg/L, adding appropriate running water after mixing makes it mix, pH to 6 ~ 8 are adjusted with acetic acid and sodium carbonate, the edible fungus species of 20wt% is accessed, any one or a few the mixture that described edible mushroom is mushroom, straw mushroom, mushroom, auricularia auriculajudae, white fungus, hedgehog hydnum, dictyophora phalloidea, Trichotoma matsutake, dried mushroom, russule, bolete, hickory chick, saddle fungus, ferfas and picks up the ears in mushroom after autoclaving.Airlift fermentor is used to carry out Submerged liquid culturation.Control throughput 20L/ hour therebetween, dissolved oxygen amount controls in DO value 10%, and fermentation period is 4 days.Will be for subsequent use after refiner beats containing mycelium fermentation broth, after testing, wherein β-1, the content of 3 glucans is more than 0.1wt%, the respective content of wood sugar, xylan and pentosan is more than 1wt%, and alpha-aminobutyric acid content is more than 0.1wt%, and glutathione content is 10 more than μ g/mL.
The preparation of 1.3 lactic acid bacteria bacteria suspensions:
The edible fungus fermented supernatant utilizing above-mentioned 1.2 preparations is culture medium, accesses the lactic acid bacteria culturers of 1v/v% after autoclaving, uses airlift fermentor to carry out Submerged liquid culturation.Control throughput 5L/ hour therebetween, dissolved oxygen amount controls, in DO value 2%, to ferment to 10
9namely CFU/mL stops fermentation, under fermentation liquor 3000rpm condition after centrifugal 30 minutes, the centrifugal supernatant under centrifugal thalline similarity condition is adjusted to 10
6~ 10
12the mixed liquor of CFU/mL, or OD600 value more than 0.1, this is lactic acid bacteria bacteria suspension.Lactic acid bacteria used is the mixed in equal amounts bacterium of streptococcus thermophilus, lactobacillus bulgaricus or two kinds of bacterium.
The preparation of 1.4 micro-capsule open-mouthed baits:
A kind of compound aquatic product micro-capsule open-mouthed bait, it comprises the component of following percetage by weight:
Additionally add:
Sodium alginate 2%.
The fly-maggot protein polypeptide liquid of formula ratio, edible mushroom homogenate, lactic acid bacteria bacteria suspension, spirulina powder, soybean lecithin, water and sodium alginate are mixed, the mixing of 45 DEG C of stirring in water bath, then adds after the peanut oil of formula ratio or soya-bean oil high-speed homogenization machine beat and at once sprays into 15wt%CaCl with atomizer pressurization
2form microcapsule granule in the aqueous solution and be less than 1 millimeter of micro-capsule with making particle diameter after 80 mesh sieve filtrations.This bait is at once thrown after preparing at the scene to spread in pool to feed and is raised.
Embodiment 2:
The preparation of 2.1 fly-maggot protein polypeptide liquid:
After hatching housefly maggot 10kg clear water rinsed clean by pig, ox or chicken manure incubation, add the phosphate buffer 5000mL of 0.05M, through homogenizer homogeneous 2 times under 20 ~ 40MPa condition, obtain homogeneous emulsion; Emulsion under 6000rpm condition centrifugal 20 minutes, get supernatant to preserve, centrifugation adds the mixing of same volume 0.05M phosphate buffer, is adjusted to pH3 with phosphoric acid, adds acid protease in 37 DEG C of insulations 3 hours, pH8 is adjusted to again with NaOH, equally 37 DEG C of insulations 3 hours, enzymolysis product mixes with aforementioned centrifuged supernatant, and the phosphate buffer adjustment protein concentration adding 0.05M is not less than 10mg/mL, amino nitrogen content is not less than 10mg/100mL, obtains fly-maggot protein polypeptide solution.
The preparation of 2.2 edible fungus fermented homogenates:
Get brewex's grains 20wt%, wheat bran 5wt%, analysis for soybean powder 0.6wt%, coarse rice powder 8wt%, sucrose 2wt%, dipotassium hydrogen phosphate 0.2wt%, magnesium sulfate 0.15wt%, CaCl
21.5wt%, vitamin B12 0mg/L, adding appropriate running water after mixing makes it mix, PH to 6 ~ 8 are adjusted with acetic acid and sodium carbonate, the edible fungus species of 20wt% is accessed, any one or a few the mixture that described edible mushroom is mushroom, straw mushroom, mushroom, auricularia auriculajudae, white fungus, hedgehog hydnum, dictyophora phalloidea, Trichotoma matsutake, dried mushroom, russule, bolete, hickory chick, saddle fungus, ferfas and picks up the ears in mushroom after autoclaving.Airlift fermentor is used to carry out Submerged liquid culturation.Control throughput 20L/ hour therebetween, dissolved oxygen amount controls in DO value 20%, and fermentation period is 5 days.Will be for subsequent use after refiner beats containing mycelium fermentation broth, after testing, wherein β-1, the content of 3 glucans is more than 0.1wt%, the respective content of wood sugar, xylan and pentosan is more than 1wt%, and alpha-aminobutyric acid content is more than 0.1wt%, and glutathione content is 10 more than μ g/mL.
2.3 lactic acid bacteria collecting cells:
The edible fungus fermented supernatant utilizing above-mentioned 2.2 preparations is culture medium, accesses the lactic acid bacteria culturers of 2v/v% after autoclaving, uses airlift fermentor to carry out Submerged liquid culturation.Control throughput 8L/ hour therebetween, dissolved oxygen amount controls, in DO value 5%, to ferment to 10
9namely CFU/mL stops fermentation.After centrifugal 20 minutes, the centrifuged supernatant under centrifugal thalline similarity condition is adjusted to 10 under fermentation liquor 6000rpm condition
10cFU/mL, or OD
600the bacteria suspension of value more than 0.5, this is lactic acid bacteria bacteria suspension.Lactic acid bacteria used is the mixed in equal amounts bacterium of streptococcus thermophilus, lactobacillus bulgaricus or two kinds of bacterium.
The preparation of 2.4 micro-capsule open-mouthed baits:
A kind of compound aquatic product micro-capsule open-mouthed bait, it comprises the component of following percetage by weight:
Additionally add:
Sodium alginate 3%.
The fly-maggot protein polypeptide liquid of formula ratio, edible mushroom homogenate, lactic acid bacteria bacteria suspension, spirulina powder, soybean lecithin, water and sodium alginate are mixed, the mixing of 45 DEG C of stirring in water bath, then adds after the peanut oil of formula ratio or soya-bean oil high-speed homogenization machine beat and at once sprays into 20wt%CaCl with atomizer pressurization
2form microcapsule granule in the aqueous solution and be less than 1 millimeter of micro-capsule with making particle diameter after 80 mesh sieve filtrations.This bait can be stored in be preserved and uses for a long time after Co60 irradiates after airtight packing in the fresh-keeping solution of 15wt% calcium chloride, 10wt% calcium propionate composition, also can grind packing after sieving after drying and preserve.
Embodiment 3:
The ratio of to get in above-described embodiment the bait of 1,2 preparations mixes rear nutrition purposes, especially for feeding animals in the ratio of 15 ㎏/ton natural feed and throws something and feeds that (control group is conventional bait to bait: spirulina, yeast, Che Yuan, yolk etc.; Experimental group: conventional bait is with 25%, and microcapsule of the present invention is with 75%), origin operation code is constant, is still three times on the one: early (5:00), evening (15:00), night (when zero) or morning, late twice, also can use according to local feeding method.
Growing state after the use of routine observation animal: body weight gathers way, the death rate, survival rate and animal development situation (as change flea shape I, the II of prawn, change chaff phase, abnormal time), and make comparisons with control group (all the other are identical except not adding open-mouthed bait), the long growth rate experimental result of prawn body is in table 1.
Table 1
Find that experimental group survival rate is apparently higher than control group according to another observed result, and shrimp seedling vigor is large, growth also fast.Become chaff phase control group and used 59 hours, experimental group has used 56 hours.Period of metamorphosis, control group has used 54 hours, and experimental group has used 51 hours, and survival rate is always higher than control group 10 percentage points.
Prove by experiment: experimental group survival rate is higher than control group, and growth also fast, the abnormal time needed is short, and seedling is neat, Miao Zhuan.
Breeding experiment end product: experimental group 1,500,000 tail prawn larvae is bred as young shrimp 500,000 tail, control group 4,000 ten thousand tail is bred as young shrimp 2,000,000 tail.
Claims (5)
1. a compound aquatic product micro-capsule open-mouthed bait, is characterized in that, it comprises the component of following percetage by weight:
Above weight percentages of components sum is 100%;
Additionally add:
Sodium alginate 1 ~ 3%;
Wherein, described fly-maggot protein polypeptide liquid, protein concentration is wherein more than 10mg/mL, and amino nitrogen content is more than 10mg/100mL, is less than 1mg/mL to the bacteriostatic activity of Escherichia coli and salmonella; Described fly-maggot protein polypeptide liquid prepares as follows: after being mixed with phosphate buffer by fly maggot polypide, high-speed homogenization is smashed, get supernatant after centrifugal to preserve, precipitation continues to mix with phosphate buffer, with phosphoric acid or acetic acid adjust pH to 1 ~ 4, add acid protease in 37 DEG C of enzymolysis 1 ~ 3 hour, use NaOH adjust pH to 7 ~ 10 again, then 37 DEG C of enzymolysis 1 ~ 3 hour, enzymolysis liquid mixed with described supernatant and get final product; Described fly maggot is for adopting pig, ox or chicken manure incubation hatching housefly maggot;
Wherein, described edible mushroom homogenate, wherein the content of β-1,3 glucan is more than 0.1wt%, and the respective content of wood sugar, xylan and pentosan is more than 1wt%, and alpha-aminobutyric acid content is more than 0.1wt%, and glutathione content is 10 more than μ g/mL; Described edible mushroom homogenate prepares as follows: edible mushroom seed liquor added in culture medium by the inoculum concentration of 10 ~ 30wt% and carry out liquid deep layer fermenting and cultivate and zymotic fluid and mycelium thereof to be beaten through refiner after 3 ~ 7 days and get final product, control throughput 5 ~ 35L/ hour during fermented and cultured, dissolved oxygen amount controls in DO value 10 ~ 30%; Wherein, described culture medium comprises the component of following percetage by weight: brewex's grains 5 ~ 30%, wheat bran 1 ~ 5%, analysis for soybean powder 0.05 ~ 1.0%, coarse rice powder 1 ~ 9%, sucrose 1 ~ 3%, dipotassium hydrogen phosphate 0.05 ~ 0.5%, magnesium sulfate 0.03 ~ 0.3%, CaCl
20.1 ~ 3.0%, Cobastab
110 ~ 50mg/L, all the other are water, pH6 ~ 8;
Wherein, in described lactic acid bacteria bacteria suspension, the content of lactic acid bacteria is 10
6~ 10
12cFU/mL.
2. compound aquatic product micro-capsule open-mouthed bait according to claim 1, it is characterized in that, any one or a few the mixture that described edible mushroom is mushroom, straw mushroom, mushroom, auricularia auriculajudae, white fungus, hedgehog hydnum, dictyophora phalloidea, Trichotoma matsutake, dried mushroom, russule, bolete, hickory chick, saddle fungus, ferfas and picks up the ears in mushroom.
3. the preparation method of compound aquatic product micro-capsule open-mouthed bait according to claim 1, it is characterized in that, the fly-maggot protein polypeptide liquid of formula ratio, edible mushroom homogenate, lactic acid bacteria bacteria suspension, spirulina powder, soybean lecithin, water and sodium alginate are mixed, the mixing of 40 ~ 60 DEG C of stirring in water bath, then adds after the peanut oil of formula ratio or soya-bean oil high-speed homogenization machine beat and at once sprays into 15 ~ 20wt%CaCl with atomizer pressurization
2form microcapsule granule in the aqueous solution and be less than 1 millimeter of micro-capsule with making particle diameter after 80 mesh sieve filtrations.
4. the preparation method of compound aquatic product micro-capsule open-mouthed bait according to claim 3, it is characterized in that, described peanut oil or the formula ratio of soya-bean oil select more than 2 different amounts, prepare the bait of more than 2 batches, make the proportion of bait different thus are suspended in the different depth of water.
5. compound aquatic product micro-capsule open-mouthed bait according to claim 1 is preparing the application in aquatic seedling open-mouthed bait.
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