CN102018094B - Product of microorganism fermented forage - Google Patents
Product of microorganism fermented forage Download PDFInfo
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- CN102018094B CN102018094B CN201010554293XA CN201010554293A CN102018094B CN 102018094 B CN102018094 B CN 102018094B CN 201010554293X A CN201010554293X A CN 201010554293XA CN 201010554293 A CN201010554293 A CN 201010554293A CN 102018094 B CN102018094 B CN 102018094B
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- Fodder In General (AREA)
Abstract
The invention discloses a product of microorganism fermented forage. The microorganism fermented forage contains thallus and mycoplasma obtained by fermenting a schizophyllum solid. The culturing temperature of a fermentation stage of the schizophyllum solid is 20-27 DEG C; after the schizophyllum commune solid is cultured for 5-8 days, the temperature is reduced to 6-10 DEG C; after the schizophyllum commune solid is cultured for 1-3 days, the temperature is raised to 20-27 DEG C; and the total culturing time is for 15-40 days. The product has stable performance and safe use and can be matched with other forages without contraindication. The amount of the product added to the forage is 20-100%; after the product is foddered, the immunity and the milking capacity of an animal body can be remarkably improved; the daily weight gain and the forage transformation rate are enhanced; and the product satisfies the green production requirement on animal food and has very remarkable social benefit and economic benefit.
Description
Technical field
Belong to microorganism field, particularly the fungi fermentation field of fodder
Background technology:
Schizophyllum commune (Schizophyllum commune (Fr.) Fr.) has another name called white ginseng, tree flower, smallpox bacterium etc., Schizophyllum Eumycota, Basidiomycotina, Hymenomycetes, Aphyllophorales, schizophyllum commune section, Schizophyllum.Schizophyllum commune is the traditional edible and medicinal fungi of a kind of China, contains the active ingredients such as Schizophyllum commune Fr polysaccharides, nucleosides, adenosine, purine.In the schizophyllum commune born of the same parents and the outer polysaccharide of born of the same parents can promote the part of macrophage to move and strengthen its activity, also can inducing T cell, the cytohormone of NK cell and many and Immune interrelation produces, and has strong antitumor activity.Schizophyllum commune is flat, flavor is sweet, can clearing liver, make eye bright, lactagogue, nourishing and fit keeping function, and can treat women's leukorrhea, lassitude, dizzy tinnitus, abnormal sweating.Schizophyllum commune is mainly cultivated the dual mode acquisition by artificial cultivation and submerged fermentation at present, and it is short that the schizophyllum commune fermented and cultured has the cycle, the characteristics that output is large.
At present, the direction of domestic research to schizophyllum commune has: Schizophyllum commune Fr polysaccharides extracts to be identified and the research of polysaccharide pharmacological effect, the research of submerged fermentation condition, and the research of schizophyllum commune artificial cultivation method utilizes the research of schizophyllum commune production malic acid.The schizophyllum commune product is less report, develops as early as possible schizophyllum commune class Related product, is a good thing that promotes the well-being of mankind.
Solid fermentation is the effective ways that obtain schizophyllum commune.Resulting mycoplasma contains the metabolite of thalline behind the solid fermentation, and mycoplasma itself also is good nutriment.The mycoplasma that solid fermentation obtains and thalline are to have nutrition and medicinal function, are having a good application prospect aspect the Application and Development of natural feed addictive.
Feed additive industry is along with China cultivation industry flourish also obtained development at full speed, in order to improve the growth of animal performance, reduce aquaculture cost, comprise antibiotic, the feed addictives such as steroids growth promoter (Clenbuterol and testosterone etc.) once or continue to be used widely, they have made huge contribution to the development of animal husbandry.But the negative effect of the use that this type of feed addictive is long-term, a large amount of also comes out day by day significantly.Be in particular in: the chemical sproof generation of livestock and poultry and human body, the materials such as antibiotic form residual at body, and animal immunizing power descends, and human body produces many bad reactions etc., and the calendar year 2001 outlet that meets with of the Livestock Products in China main cause of being obstructed is exactly antibiotic residual.
At present, the internal feed additives industries is in a transitional period, low-residual or noresidue, without the resistance to the action of a drug, natural green feed additive will replace gradually antibiotic and other has the feed addictive of residual contamination problem.Green feed additive, must possess " can strengthen animal immunity, can obviously improve the conversion ratio of feed and quantum of output and the environmentally safe of animal " etc. the three basic requirement.The use of green feed additive is the basis that ensures animal food safety.It meets the developing direction of feed addictive, and green feed additive will become the main product in feed addictive market, and the use of green feed additive is the primary condition that ensures China's meat products safety.
At present, the green feed additive of China is to some extent development also, and active bacteria formulation, saccharicter-penin, compound sugar, fodder enzyme preparation, Chinese herbal feed additive are main several.There are the problems such as product standard lacks, technical merit is on the low side, the product function composition is indefinite, addition is large in green feed additive at present.Stable and controllable for quality, noresidue, non-environmental-pollution, use safety, not limited by raw material, the green feed additive product that properties of product are stable is anxious to be waited to develop.
Summary of the invention:
The technical problem that the present invention solves has provided a kind of product of microorganism fermented forage.
Product of the present invention is comprised of thalline and the mycoplasma that the schizophyllum commune solid fermentation obtains, and the production process of product of the present invention is as follows: solid fermentation is cultivated the schizophyllum commune culture that obtains pulverize drying, obtain the crushed material of dry powdery, packing is product.
Product processes step of the present invention comprises the steps: seed culture: shaking table is cultivated the schizophyllum commune liquid seeds; Solid fermentation: switching schizophyllum commune liquid seeds is to solid fermentation culture medium fermented and cultured; Drying and crushing: it is complete to ferment, drying solid fermentation medium on fluid bed or other drying equipments, dry complete the pulverizing; Packing: the direct packing of comminuting matter both can.
20-27 ℃ of described solid fermentation cultivation stage cultivation temperature, humidity 75-90%, ventilation 0.3-1.0 (V/V), total incubation time 15-40 days; Cultivate after 5-8 days, reduce the temperature to 6-10 ℃, cultivated 1-3 days.
Schizophyllum commune production of fodder process is as follows:
(1) slant strains activation culture: the slant strains of preservation is transferred on the slant medium, cultivated about 96～120 hours, and covered with the inclined-plane to mycelia and get final product for 22～28 ℃; 25 ℃ of optimum culturing temperatures.
(2) the liquid first order seed is cultivated: access of above-mentioned slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate, condition of culture: 80～180 rev/mins of rotary shaking tables, cultivated about 96～130 hours for 22～28 ℃; 150 rev/mins of rotary shaking tables are advisable, and cultivation temperature is advisable with 25 ℃.
(3) liquid two stage seed culture: the one-level shake-flask seed is equipped with 5～20% inoculum concentrations accesses carries out secondary seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate, condition of culture: 80～180 rev/mins of rotary shaking tables, cultivated about 96～130 hours for 22～28 ℃; Suitable inoculum concentration is 10%, and 150 rev/mins of rotary shaking tables are advisable, and cultivation temperature is advisable with 25 ℃.
(4) solid fermentation is cultivated: the secondary shake-flask seed is equipped with in the 500L solid-state fermentation tank of the rear solid fermentation culture medium of sterilization with the access of 5～10% inoculum concentrations, condition of culture: charge 50%, cultivation temperature 20-27 ℃, cultivate after 5-8 days, reduce the temperature to 6-10 ℃, humidity 65-80%, ventilation 0.1-0.5 (V/V), incubation time 1-3 days.Improve subsequently temperature to 20-27 ℃, humidity 75-90%, ventilation 0.3-1.0 (V/V), total incubation time 15-40 days.Opened every 3-10 days in case of necessity and stirred 10 minutes;
(5) fermented and cultured finishes, and selects the multiple drying means such as fluid bed, and dry materials is arrived moisture below 7%.The solid fermentation material is pulverized.
(6) packing.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The carbon source of solid fermentation culture medium of the present invention is mainly selected the raw materials commonly used such as wheat bran, glucose, sucrose, starch, corn flour, corncob (after pulverizing), and nitrogenous source can be selected groundnut meal, peptone, De-fatted soya protein Powder, beancake powder, dusty yeast etc.; The inorganic salts that need to add have potassium dihydrogen phosphate, magnesium sulfate.
The agent of liquid fermentation seed culture forms among the present invention: starch 2～3%, sucrose 3～5%, glucose 1～3%, peptone 0.5%, De-fatted soya protein Powder 2～3%, potassium dihydrogen phosphate 0.13～0.2%, magnesium sulfate 0.1～0.15%, Cobastab
12PPm, PH6～7.
The schizophyllum commune cultural method is fit to belong to together other bacterial classification and carries out large scale fermentation by fermentation method and cultivate to make feed among the present invention.
Beneficial effect:
Studies show that by experiment: the schizophyllum commune feed product has good lactagogue effect;
Safety is used in the stable performance of product of the present invention, with other feed all without incompatibility.The addition of product of the present invention in feed is 20-100%, feeding after, can significantly strengthen immunity and the milking capacity of animal body, improve daily gain and feed conversion rate.According to test, the animal milk water secretory volume of feeding feed of the present invention significantly improves, and meets the greenization production requirement of animal food, and Social benefit and economic benefit is very remarkable.
Product schizophyllum commune feed product of the present invention is take Chinese traditional edible and medical fungi of biological fermentation process production as primary raw material, constant product quality is controlled, in vivo noresidue of product, non-environmental-pollution, without the resistance to the action of a drug, and can improve immunity, can improve culture benefit.Product meets the requirement of green feed additive fully.
Protein content 10-16% in the product of the present invention, polyoses content reaches 8-12%, contains simultaneously vitamin E, B1, B2.Lysine, each seed amino acid that the human bodies such as glutamic acid are necessary and various trace element.Product has abundant nutritive value.
Because schizophyllum commune has higher nutritive value and bacteria resistance function, can strengthen the animal lactation ability, and the noresidue environmentally safe.Produce schizophyllum commune and it is processed as feed by biofermentation technique, the developing direction that meets current feed fully, it is a kind of green feed, particularly adopt cooling of the present invention stimulated production the method effective stimulus schizophyllum commune promote the generation of lactation material, stimulate product to compare with non-cooling, product of the present invention can improve lactation quantity more than 15%, and effect is comparatively obvious.Adopt biofermentation technique or artificial cultivation technique large-scale production schizophyllum commune natural feed; have stable and controllable for qualityly, noresidue, non-environmental-pollution, use safety, not limited by raw material, properties of product are stable; with low cost, be convenient to the schizophyllum commune feed in the promotion and application of feedstuff industry.
Milking capacity effect effect experiment.
Respectively two groups of milk cows are fed experiment, fed 40 days, check each time period milk crop, the results are shown in Table 1.
Each time period milk crop experimental unit: kg of table 1 raising dairy cattle
The specific embodiment:
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Embodiment 1 (schizophyllum commune feed product)
A kind of schizophyllum commune feed product mainly is comprised of the Schizophyllum commune for Mycelium after pulverizing and the dry solid content in the schizophyllary fermentating liquid, this routine product exists with powder-form, formed by schizophyllum commune solid fermentation thalline and mycoplasma in the product, the production process of product of the present invention is as follows: the schizophyllum commune solid fermentation is cultivated, the schizophyllum commune solid fermentation culture is pulverized dry, and packing is product.
Embodiment 2
Schizophyllum commune feed product production process is as follows:
(1) slant strains activation culture: the schizophyllum commune slant strains of preservation is transferred on the slant medium, cultivated about 96 hours, and covered with the inclined-plane to mycelia and get final product for 22 ℃;
(2) the liquid first order seed is cultivated: access of above-mentioned schizophyllum commune slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 150 rev/mins of rotary shaking tables, cultivated about 130 hours for 25 ℃;
(3) liquid two stage seed culture: the one-level shake-flask seed is equipped with 5% inoculum concentration access carries out secondary seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 100 hours for 25 ℃;
(4) solid fermentation is cultivated: the secondary shake-flask seed is equipped with in the 500L solid-state fermentation tank of the rear solid fermentation culture medium of sterilization with the access of 10% inoculum concentration, condition of culture: charge 50%, 24 ℃ of cultivation temperature, cultivate after 8 days, reduce the temperature to 6 ℃, humidity 65-80%, ventilation 0.2-0.3 (V/V), incubation time 3 days.Improve subsequently temperature to 26 ℃, humidity 75-90%, ventilation 0.8-1.0 (V/V), total incubation time 26 days.Opened every 3-10 days in case of necessity and stirred 10 minutes;
(5) fermented and cultured finishes, and the solid fermentation material is selected the multiple drying means dryings such as fluid bed, and baking temperature is controlled at below 80 ℃.Dry materials is arrived moisture below 7%.The material that drying is complete is pulverized.
(6) packing of product.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The solid fermentation culture medium is composed as follows among the present invention: 70% wheat bran, 1.4% sucrose, 10% corn flour, 16% corncob (after pulverizing), 0.1% peptone, 2% De-fatted soya protein Powder, 0.5% dusty yeast; Culture medium PH nature.121 ℃ of sterilising conditions 2 hours.
The liquid fermentation seed culture medium forms among the present invention: starch 2%, glucose 3%, peptone 0.3%, De-fatted soya protein Powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, vitamin B1 2PPm, PH6.Schizophyllum commune (Schizophyllumcommune Fr.) bacterial classification cfcc 82418 is purchased from China Forest microorganism fungus kind preservation administrative center.
Embodiment 3
Schizophyllum commune production of fodder process is as follows:
(1) slant strains activation culture: the schizophyllum commune slant strains of preservation is transferred on the slant medium, cultivated about 96 hours, and covered with the inclined-plane to mycelia and get final product for 28 ℃;
(2) the liquid first order seed is cultivated: access of above-mentioned schizophyllum commune slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 96 hours for 28 ℃;
(3) liquid two stage seed culture: the one-level shake-flask seed is equipped with 5% inoculum concentration access carries out secondary seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 96 hours for 28 ℃;
(4) solid fermentation is cultivated: the secondary shake-flask seed is equipped with in the 500L solid-state fermentation tank of the rear solid fermentation culture medium of sterilization with the access of 10% inoculum concentration, condition of culture: charge 50%, 22 ℃ of cultivation temperature, cultivate after 8 days, reduce the temperature to 7 ℃, humidity 65-80%, ventilation 0.2-0.3 (V/V), incubation time 1 day.Improve subsequently temperature to 25 ℃, humidity 75-90%, ventilation 0.8-1.0 (V/V), total incubation time 35 days.Opened every 3-10 days in case of necessity and stirred 10 minutes.
(5) fermented and cultured finishes, and the solid fermentation material is selected the multiple drying means dryings such as fluid bed, and baking temperature is controlled at below 80 ℃.Dry materials is arrived moisture below 7%.The material that drying is complete is pulverized.
(6) packing of product.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The solid fermentation culture medium is composed as follows among the present invention: 80% wheat bran, 1.4% sucrose, 8% corn flour, 8% corncob (after pulverizing), 0.1% peptone, 2% De-fatted soya protein Powder, 0.5% dusty yeast; Culture medium PH nature.121 ℃ of sterilising conditions 2 hours.The liquid fermentation seed forms among the present invention: starch 2%, glucose 3%, peptone 0.3%, De-fatted soya protein Powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, Cobastab
12PPm, pH6.Bacterial classification: schizophyllum commune CPCC810155 (Schizophyllum commune) is purchased from Chinese medicinal microorganism fungus kind preservation administrative center.
Claims (2)
1. product of microorganism fermented forage, the thalline and the mycoplasma that are obtained by the schizophyllum commune solid fermentation form, it is characterized in that, described schizophyllum commune solid fermentation stage cultivation temperature is 20-27 ℃, cultivate after 5-8 days, reduce the temperature to 6-10 ℃, cultivated 1-3 days, improve subsequently temperature to 20-27 ℃, total incubation time 15-40 days.
2. the production method of product of microorganism fermented forage as claimed in claim 1 is characterized in that described schizophyllum commune solid fermentation stage cultivation temperature is 20-27 ℃, cultivate after 5-8 days, reduce the temperature to 6-10 ℃, cultivated 1-3 days, improve subsequently temperature to 20-27 ℃, total incubation time 15-40 days.
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