CN102018094A - Product of microorganism fermented forage - Google Patents
Product of microorganism fermented forage Download PDFInfo
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- CN102018094A CN102018094A CN201010554293XA CN201010554293A CN102018094A CN 102018094 A CN102018094 A CN 102018094A CN 201010554293X A CN201010554293X A CN 201010554293XA CN 201010554293 A CN201010554293 A CN 201010554293A CN 102018094 A CN102018094 A CN 102018094A
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- 238000000855 fermentation Methods 0.000 claims abstract description 37
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Abstract
The invention discloses a product of microorganism fermented forage. The microorganism fermented forage contains thallus and mycoplasma obtained by fermenting a schizophyllum solid. The culturing temperature of a fermentation stage of the schizophyllum solid is 20-27 DEG C; after the schizophyllum commune solid is cultured for 5-8 days, the temperature is reduced to 6-10 DEG C; after the schizophyllum commune solid is cultured for 1-3 days, the temperature is raised to 20-27 DEG C; and the total culturing time is for 15-40 days. The product has stable performance and safe use and can be matched with other forages without contraindication. The amount of the product added to the forage is 20-100%; after the product is foddered, the immunity and the milking capacity of an animal body can be remarkably improved; the daily weight gain and the forage transformation rate are enhanced; and the product satisfies the green production requirement on animal food and has very remarkable social benefit and economic benefit.
Description
Technical field
Belong to microorganism field, particularly the fungi fermentation field of fodder
Background technology:
Schizophyllum commune (Schizophyllum commune (Fr.) Fr.) has another name called white ginseng, tree flower, smallpox bacterium etc., Schizophyllum Eumycota, Basidiomycotina, Hymenomycetes, Aphyllophorales, schizophyllum commune section, Schizophyllum.Schizophyllum commune is the traditional edible and medicinal fungi of a kind of China, contains active ingredients such as Schizophyllum commune Fr polysaccharides, nucleosides, adenosine, purine.In the schizophyllum commune born of the same parents and the outer polysaccharide of born of the same parents can promote the part of macrophage to move and strengthen its activity, also can inducing T cell, NK cell and the generation of many and immune relevant cytohormone, have strong antitumor activity.Schizophyllum commune is flat, flavor is sweet, can clearing liver, make eye bright, lactagogue, nourishing and fit keeping function, and can treat women's leukorrhea, lassitude, dizzy tinnitus, abnormal sweating.Schizophyllum commune is mainly cultivated the dual mode acquisition by artificial cultivation and submerged fermentation at present, and it is short that the schizophyllum commune fermented and cultured has the cycle, the characteristics that output is big.
At present, the direction of domestic research to schizophyllum commune has: Schizophyllum commune Fr polysaccharides extracts to be identified and the research of polysaccharide pharmacological effect, the submerged fermentation Study on Conditions, and the research of schizophyllum commune artificial cultivation method utilizes the research of schizophyllum commune production malic acid.The less report of schizophyllum commune product is developed schizophyllum commune class Related product as early as possible, is a good thing that promotes the well-being of mankind.
Solid fermentation is the effective ways that obtain schizophyllum commune.Resulting mycoplasma contains the metabolite of thalline behind the solid fermentation, and mycoplasma itself also is good nutriment.Mycoplasma that solid fermentation obtains and thalline are to have nutrition and medicinal function, are having a good application prospect aspect the Application and Development of natural feed addictive.
Feed additive industry has flourishly also obtained development at full speed along with what industry was cultured by China, in order to improve the growth of animal performance, reduce aquaculture cost, comprise antibiotic, steroids growth promoter feed addictives such as (Clenbuterol and testosterones etc.) once or continue to be used widely, they have made huge contribution to Developing of Animal Industry.But the negative effect of the use that this type of feed addictive is long-term, a large amount of also comes out day by day significantly.Be in particular in: the chemical sproof generation of livestock and poultry and human body, materials such as antibiotic form residual at body, and animal immunizing power descends, and human body produces many bad reactions etc., and the calendar year 2001 outlet that meets with of the China's livestock products main cause of being obstructed is exactly a residues of antibiotics.
At present, internal feed additive industry is in a transitional period, and low-residual or noresidue, the no resistance to the action of a drug, natural green feed additive will replace antibiotic gradually and other has the feed addictive of residual contamination problem.Green feed additive, must possess " can strengthen animal immunity, can obviously improve the conversion ratio of feed and quantum of output and the environmentally safe of animal " etc. three basic demands.The use of green feed additive is the basis that ensures animal food safety.It meets the developing direction of feed addictive, and green feed additive will become the main product in feed addictive market, and the use of green feed additive is the primary condition that ensures China's meat products safety.
At present, the green feed additive of China is development to some extent also, and active bacteria formulation, saccharicter-penin, compound sugar, fodder enzyme preparation, Chinese herbal feed additive are main several.There are problems such as product standard lacks, technical merit is on the low side, the product function composition is indeterminate, addition is big in green feed additive at present.Stable and controllable for quality, noresidue, non-environmental-pollution, safe in utilization, not limited by raw material, the green feed additive product that properties of product are stable is anxious to be waited to develop.
Summary of the invention:
The technical problem that the present invention solves has provided a kind of fermentative feedstuff of microbe product.
Product of the present invention is made up of thalline and mycoplasma that the schizophyllum commune solid fermentation obtains, and the production process of product of the present invention is as follows: solid fermentation is cultivated the schizophyllum commune culture that obtains pulverize drying, obtain the crushed material of dry powdery, packing is product.
Product processes step of the present invention comprises the steps: seed culture: shaking table is cultivated the schizophyllum commune liquid seeds; Solid fermentation: switching schizophyllum commune liquid seeds is to solid fermentation culture medium fermented and cultured; Drying and crushing: fermentation finishes, drying solid fermentation medium on fluid bed or other drying equipments, and drying finishes and pulverizes; Packing: the direct packing of comminuting matter both can.
20-27 ℃ of described solid fermentation cultivation stage cultivation temperature, humidity 75-90%, ventilation 0.3-1.0 (V/V), total incubation time 15-40 days; Cultivate after 5-8 days, reduce the temperature to 6-10 ℃, cultivated 1-3 days.
Schizophyllum commune production of fodder process is as follows:
(1) slant strains activation culture: the slant strains of preservation is transferred on the slant medium, cultivated about 96～120 hours, and covered with the inclined-plane to mycelia and get final product for 22～28 ℃; 25 ℃ of optimum culturing temperatures.
(2) the liquid first order seed is cultivated: access of above-mentioned slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 80～180 rev/mins of rotary shaking tables, cultivated about 96～130 hours for 22～28 ℃; Rotary shaking table is advisable for 150 rev/mins, and cultivation temperature is advisable with 25 ℃.
(3) the liquid secondary seed is cultivated: the one-level shake-flask seed is inserted with 5～20% inoculum concentrations carry out the secondary seed cultivation in 500 ml shake flasks that 100 milliliters of culture mediums are housed, condition of culture: 80～180 rev/mins of rotary shaking tables, cultivated about 96～130 hours for 22～28 ℃; Suitable inoculum concentration is 10%, and rotary shaking table is advisable for 150 rev/mins, and cultivation temperature is advisable with 25 ℃.
(4) solid fermentation is cultivated: insert the secondary shake-flask seed in the 500L solid-state fermentation tank that sterilization back solid fermentation culture medium is housed with 5～10% inoculum concentrations, condition of culture: charge 50%, cultivation temperature 20-27 ℃, cultivate after 5-8 days, reduce the temperature to 6-10 ℃, humidity 65-80%, ventilation 0.1-0.5 (V/V), incubation time 1-3 days.Improve temperature subsequently to 20-27 ℃, humidity 75-90%, ventilation 0.3-1.0 (V/V), total incubation time 15-40 days.Opened every 3-10 days in case of necessity and stirred 10 minutes;
(5) fermented and cultured finishes, and selects multiple drying means such as fluid bed, and dry materials is arrived moisture below 7%.The solid fermentation material is pulverized.
(6) packing.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The carbon source of solid fermentation culture medium of the present invention is mainly selected wheat bran, glucose, sucrose, starch, corn flour, corncob raw materials commonly used such as (pulverizing the back), and nitrogenous source can be selected groundnut meal, peptone, skimmed soybean protein powder, soya-bean cake powder, dusty yeast etc.; The inorganic salts that need to add have potassium dihydrogen phosphate, magnesium sulfate.
The agent of liquid fermentation seed culture is formed among the present invention: starch 2～3%, sucrose 3～5%, glucose 1～3%, peptone 0.5%, skimmed soybean protein powder 2～3%, potassium dihydrogen phosphate 0.13～0.2%, magnesium sulfate 0.1～0.15%, Cobastab
12PPm, PH6～7.
The schizophyllum commune cultural method is fit to belong to together other bacterial classification and carries out large scale fermentation by fermentation method and cultivate to make feed among the present invention.
Beneficial effect:
Studies show that by experiment: the schizophyllum commune feed product has good lactagogue effect;
The stable performance of product of the present invention, safe in utilization, all there is not incompatibility with other feed.The addition of product of the present invention in feed is 20-100%, feeding after, can significantly strengthen the immunity and the milking capacity of animal body, improve daily gain and feed conversion rate.According to test, the animal milk water secretory volume of feeding feed of the present invention significantly improves, and meets the greenization production requirement of animal food, and social benefit and economic benefit are very remarkable.
Product schizophyllum commune feed product of the present invention is a primary raw material with Chinese traditional edible and medical fungi of biological fermentation process production, constant product quality is controlled, product noresidue in vivo, non-environmental-pollution, the no resistance to the action of a drug, and can improve immunity, can improve culture benefit.Product meets the requirement of green feed additive fully.
Protein content 10-16% in the product of the present invention, polyoses content reaches 8-12%, contains vitamin E simultaneously, B1, B2.Lysine, each seed amino acid that human bodies such as glutamic acid are necessary and various trace element.Product has abundant nutritive value.
Because schizophyllum commune has higher nutritive value and bacteria resistance function, can strengthen the animal milking capacity, and the noresidue environmentally safe.Produce schizophyllum commune and it is processed as feed by biofermentation technique, the developing direction that meets current feed fully, it is a kind of green feed, particularly adopt cooling of the present invention stimulated production the method effective stimulus schizophyllum commune promote the generation of lactation material, stimulate product to compare with non-cooling, product of the present invention can improve lactation quantity more than 15%, and effect is comparatively obvious.Adopt biofermentation technique or artificial cultivation technique large-scale production schizophyllum commune natural feed; have stable and controllable for quality, noresidue, non-environmental-pollution, safe in utilization, not limited by raw material, properties of product are stable; with low cost, be convenient to of the promotion and application of schizophyllum commune feed at feedstuff industry.
Milking capacity effect effect experiment.
Respectively two groups of milk cows are fed experiment, fed 40 days, check each time period milk crop, the results are shown in Table 1.
Each time period milk crop experimental unit: kg of table 1 raising dairy cattle
The specific embodiment:
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment 1 (schizophyllum commune feed product)
A kind of schizophyllum commune feed product mainly is made up of schizophyllum commune mycelium after pulverizing and the dry solid content in the schizophyllary fermentating liquid, this routine product exists with powder-form, form by schizophyllum commune solid fermentation thalline and mycoplasma in the product, the production process of product of the present invention is as follows: the schizophyllum commune solid fermentation is cultivated, the schizophyllum commune solid fermentation culture is pulverized dry, and packing is product.
Embodiment 2
Schizophyllum commune feed product production process is as follows:
(1) slant strains activation culture: the schizophyllum commune slant strains of preservation is transferred on the slant medium, cultivated about 96 hours, and covered with the inclined-plane to mycelia and get final product for 22 ℃;
(2) the liquid first order seed is cultivated: access of above-mentioned schizophyllum commune slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 150 rev/mins of rotary shaking tables, cultivated about 130 hours for 25 ℃;
(3) the liquid secondary seed is cultivated: the one-level shake-flask seed is inserted with 5% inoculum concentration carry out the secondary seed cultivation in 500 ml shake flasks that 100 milliliters of culture mediums are housed, condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 100 hours for 25 ℃;
(4) solid fermentation is cultivated: insert the secondary shake-flask seed in the 500L solid-state fermentation tank that sterilization back solid fermentation culture medium is housed with 10% inoculum concentration, condition of culture: charge 50%, 24 ℃ of cultivation temperature, cultivate after 8 days, reduce the temperature to 6 ℃, humidity 65-80%, ventilation 0.2-0.3 (V/V), incubation time 3 days.Improve temperature to 26 ℃, humidity 75-90%, ventilation 0.8-1.0 (V/V), total incubation time 26 days subsequently.Opened every 3-10 days in case of necessity and stirred 10 minutes;
(5) fermented and cultured finishes, and the solid fermentation material is selected multiple drying means dryings such as fluid bed, and baking temperature is controlled at below 80 ℃.Dry materials is arrived moisture below 7%.The material that drying finishes is pulverized.
(6) packing of product.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The solid fermentation culture medium is composed as follows among the present invention: 70% wheat bran, 1.4% sucrose, 10% corn flour, 16% corncob (pulverizing the back), 0.1% peptone, 2% skimmed soybean protein powder, 0.5% dusty yeast; Culture medium PH nature.121 ℃ of sterilising conditions 2 hours.
The liquid fermentation seed culture medium is formed among the present invention: starch 2%, glucose 3%, peptone 0.3%, skimmed soybean protein powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, vitamin B1 2PPm, PH6.Schizophyllum commune (Schizophyllumcommune Fr.) bacterial classification cfcc 82418 purchases in China Forest microorganism fungus kind preservation administrative center.
Embodiment 3
Schizophyllum commune production of fodder process is as follows:
(1) slant strains activation culture: the schizophyllum commune slant strains of preservation is transferred on the slant medium, cultivated about 96 hours, and covered with the inclined-plane to mycelia and get final product for 28 ℃;
(2) the liquid first order seed is cultivated: access of above-mentioned schizophyllum commune slant strains picking is equipped with carries out first order seed in 500 ml shake flasks of 100 milliliters of culture mediums and cultivate condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 96 hours for 28 ℃;
(3) the liquid secondary seed is cultivated: the one-level shake-flask seed is inserted with 5% inoculum concentration carry out the secondary seed cultivation in 500 ml shake flasks that 100 milliliters of culture mediums are housed, condition of culture: 180 rev/mins of rotary shaking tables, cultivated about 96 hours for 28 ℃;
(4) solid fermentation is cultivated: insert the secondary shake-flask seed in the 500L solid-state fermentation tank that sterilization back solid fermentation culture medium is housed with 10% inoculum concentration, condition of culture: charge 50%, 22 ℃ of cultivation temperature, cultivate after 8 days, reduce the temperature to 7 ℃, humidity 65-80%, ventilation 0.2-0.3 (V/V), incubation time 1 day.Improve temperature to 25 ℃, humidity 75-90%, ventilation 0.8-1.0 (V/V), total incubation time 35 days subsequently.Opened every 3-10 days in case of necessity and stirred 10 minutes.
(5) fermented and cultured finishes, and the solid fermentation material is selected multiple drying means dryings such as fluid bed, and baking temperature is controlled at below 80 ℃.Dry materials is arrived moisture below 7%.The material that drying finishes is pulverized.
(6) packing of product.
The slant medium composition can be PDA slant medium or other suitable culture mediums among the present invention.
The solid fermentation culture medium is composed as follows among the present invention: 80% wheat bran, 1.4% sucrose, 8% corn flour, 8% corncob (pulverizing the back), 0.1% peptone, 2% skimmed soybean protein powder, 0.5% dusty yeast; Culture medium PH nature.121 ℃ of sterilising conditions 2 hours.The liquid fermentation seed is formed among the present invention: starch 2%, glucose 3%, peptone 0.3%, skimmed soybean protein powder 0.5%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.1%, Cobastab
12PPm, pH6.Bacterial classification: schizophyllum commune CPCC810155 (Schizophyllum commune) purchases the medicinal microorganism fungus kind preservation administrative center in China.
Claims (2)
1. fermentative feedstuff of microbe product is characterized in that described fermentative feedstuff of microbe is made up of thalline and mycoplasma that the schizophyllum commune solid fermentation obtains.
2. according to the described fermentative feedstuff of microbe product of claim 1, it is characterized in that described schizophyllum commune solid fermentation stage cultivation temperature is 20-27 ℃, cultivate after 5-8 days, reduce the temperature to 6-10 ℃, cultivated 1-3 days, and improved temperature subsequently to 20-27 ℃, total incubation time 15-40 days.
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| CN103156073A (en) * | 2011-12-13 | 2013-06-19 | 北京东方联鸣科技发展有限公司 | Nutritional supplement for postpartum dairy cows |
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