CN101305769B

CN101305769B - Preparation method of microbial fermentative feedstuff

Info

Publication number: CN101305769B
Application number: CN2008101279488A
Authority: CN
Inventors: 宁瑛
Original assignee: Inner Mongolia Foodstuff Science Research & Design Institute Co Ltd
Current assignee: Inner Mongolia Kehong Biotechnology Co., Ltd.
Priority date: 2008-07-02
Filing date: 2008-07-02
Publication date: 2010-10-27
Anticipated expiration: 2028-07-02
Also published as: CN101305769A

Abstract

The invention discloses a method for preparing a microorganism fermentation feed. The method comprises the main technological processes as follows: respectively culturing Candida utilis, Geotrichum candidum and Streptococcus faecalis with molasses in combination with a liquid microorganism culture medium as the main raw materials; mixing the Candida utilis culture solution, the Geotrichum candidum culture solution and the Streptococcus faecalis culture solution at a certain ratio to obtain a mixed bacteria solution; subjecting to solid fermentation in a semi-solid fermentation culture medium including molasses liquefied liquid, corn coat, vegetable meal, cotton seed meal, wheat bran, soybean meal and apple pomace; baking at a low temperature when the fermentation is finished; and pulverizing to obtain the microorganism fermentation feed. In the microorganism fermentation feed, the viable count of beneficial microorganism is larger than 1*10<9>cfu/g, and the phytase activity is higher than 1,400IU/kg. The feed has the functions of supplementing the number of milk cow (sheep) rumen microorganism, regulating rumen fermentation function, and improving feed conversion and utilization ratio.

Description

A kind of preparation method of fermentative feedstuff of microbe

Technical field

The present invention relates to the preparation method of the special-purpose micro-ecological feed of a kind of ruminant, belong to a kind of preparation method of ruminant tumor gastric regulating and controlling microbial fermented feed.

Background technology

Along with milk cattle cultivating industry and sheep are fattened developing rapidly of industry, breeding performonce fo animals constantly increases.But antibiotic being extensive use of caused animal body autogenous infection, drug resistance to produce.And the beneficial microbe in the cud is subjected to infringement, death or along with digest food excretes, this is damaged the microecological balance of normal flora in the animal intestinal, and breeding performonce fo animals descends, thereby has influenced the economic benefit and the social benefit of aquaculture.Utilize ruminant tumor gastric microecological balance theory and technology, the complex micro organism fungicide of the multiple beneficial microorganism being cultivated preparation is applied in the ruminant animal nutrition, can replenish micro organism quantity, keeps the cud microecological balance.And the beneficial microbe metabolism produces in cud a large amount of vitamins, active material can promote the animal intestine the digestion function of stomach; Materials such as the carbohydrate that microorganism is synthesized, amino acids can be animal growth high-quality nutriment are provided.Complicated larger molecular organicses such as starch, fat, protein, cellulose in the microbial metabolic products digestive ferment degradable feed change into the small organic molecules such as monose, disaccharide and amino acid that animal is easy to digest and assimilate in the cud; The suitableeest acidity under the cud microecological balance condition can be strengthened absorption and the utilization of animal body to various nutriments in the feed.The solid-state feed of little ecology can improve the physiological function of body, strengthens the resistance and the resistance against diseases of animal body, reduce or stop to culture in antibiotic use.At present both at home and abroad in ruminant animal nutrition research more be active bacteria formulation and fermentation culture medium thereof.Active bacteria formulation is generally prepared through in vitro culture by the single bacterial strain of saccharomyces cerevisiae, and main effect is for cud provides living cells and fermentating metabolism product, has the function that promotes ruminal digestion, improves efficiency of feed utilization and conversion ratio.Fermentation culture medium through in vitro culture fermentation preparation, has the enzymatic activity material in order to promote the animal digestion function by microorganism.

Summary of the invention

The technical problem to be solved in the present invention provides a kind of preparation method of fermentative feedstuff of microbe, this preparation method is primarily aimed to what the distinctive ruminal digestion function of milk cow (sheep) was produced both has the beneficial microorganism of being supplemented with a quantity regulating cud microecological balance, can provide the viable bacteria metabolite to promote the fermentative feedstuff of microbe of body cell breeding again.Its characteristics are pure natural, no side effects, safe and reliable, multi-functional, noresidue, free from environmental pollution.Add this feed of feeding in the 5%-10% ratio in ruminant feed after, can make milk cow in lactation period output of milk every day on average increase 2.37kg, butterfat percnetage improves 0.32%; Be used for the sheep feeding for fattening, can make daily gain on average improve 15%.

The technical problem to be solved in the present invention is realized by following scheme: a kind of preparation method of fermentative feedstuff of microbe, it is characterized in that: with molasses as liquid medium starting material to candida utili (C.utilis), geotrichum candidum (Geotrichum candidium link) and streptococcus fecalis (Streptococcus faecalis) enlarge to cultivate respectively and become to spread cultivation liquid, and by the 30%-45% candida utili liquid that spreads cultivation, the 30%-45% geotrichum candidum liquid that spreads cultivation, the spread cultivation weight ratio of liquid of 10%-30% streptococcus fecalis is mixed and made into mixed bacteria liquid, the weight ratio of mixed bacteria liquid being pressed 5%-15% adds by 5% molasses liquefier, 20% maize peel, the 10% dish dregs of rice, the 10% cottonseed dregs of rice, 20% wheat bran, 20% bean dregs, in the semi-solid ferment culture medium that 15% pomace constitutes, under 28-30 ℃ of condition, fermented 24-48 hour, to be lower than 60 ℃ of low temperature dryings, to pulverize again and be fermentative feedstuff of microbe after the fermentation ends.The beneficial microbe viable count is greater than 5 * 10 in the fermentative feedstuff of microbe ⁹Cfu/g, phytase activity is greater than 1400IU/kg.

This preparation method is described in detail in detail below:

1, bacterium liquid spreads cultivation

1. bacterial classification

Candida utili (C.utilis), geotrichum candidum (Geotrichum candidium link), streptococcus fecalis (Streptococcus faecalis).

2. raw material

Utilize sugar industry leftover bits and pieces molasses to prepare reduced sugar as liquid culture medium primary raw material, add 20% molasses, 0.04% phosphoric acid, 0.025% sulfuric acid and abundant agitation and dilution by water consumption, steam boils 30min, form molasses acidifying hydrolyzate, adjust pH is 4.8, filter after leaving standstill 10min, supernatant is as fermentation culture.

3. culture medium activates and spreads cultivation

A, candida utili

Molasses acidifying solution 20%, ammonium nitrate 0.5%, potassium dihydrogen phosphate 0.25%, sodium hydrogen phosphate 0.25%.

B, geotrichum candidum

Malt extract medium.

C, streptococcus fecalis

Molasses acidifying solution 10%, yeast extract 0.3%, potato leachate 20%.

4. the technology that spreads cultivation and parameter

A, candida utili

Fermentation medium nature PH, 5%, 30 ℃ of taking over a job bacterium liquid, the 6r/min stir culture is cultivated 24h.

B, geotrichum candidum

C, streptococcus fecalis

Fermentation medium PH 6.7,3%, 30 ℃ of taking over a job bacterium liquid, 6r/min stirs, and cultivates 18h.

2, mix

To cultivate back bacterium liquid is that the ratio of candida utili 30%-45%, geotrichum candidum 30%-45%, streptococcus fecalis 10%-30% imports in the blending tank and makes mixed bacteria liquid by weight.

3, solid formulation fermentation

1. the semi-solid ferment culture medium is formed by following weight ratio:

5% molasses liquefier, 20% maize peel, the 10% dish dregs of rice, the 10% cottonseed dregs of rice, 20% wheat bran, 20% bean dregs, 15% pomace.

2. connect bacterium and fermentation parameter

Add above-mentioned mixed bacteria liquid in the ratio of semi-solid ferment culture medium weight 5%-15% and carry out solid state fermentation.In 28-30 ℃ of condition bottom fermentation 24-48 hour.

3. the post processing of fermenting

To be lower than 60 ℃ of low temperature dryings, made the fermentative feedstuff of microbe of milk cow (sheep) lumen fermentation regulating and controlling effect after the pulverizing after the fermentation ends.

Advantage of the present invention is:

The fermentative feedstuff of microbe of the present invention's preparation is mainly spread cultivation by microorganism fungus kind, and the Liquid Culture fermentation is formed.Wherein candida utili and geotrichum candidum can increase rumen bypass protein quantity by rumen microorganism self metabolism growth, for ruminant provides the high-quality nutriment, reach the effect of volume increase.Fermentation culture medium stimulates the rumen microorganism breeding simultaneously, to improve function of rumen, promotes the digestion of cellulosic material and the utilization of propionic acid, improves feed digestibility.And the candida utili viable bacteria can be adsorbed noxious material and pathogen, reduces the gastrointestinal disease of poisonous substance, reduce or stop to culture in antibiotic use.Owing to improved ruminant tumor gastric fermentation function; thereby reduce the discharging of poisonous and harmful substances such as ammoniacal nitrogen, methane; therefore the environmental pollution that reduction causes because of large-scale cultivation is the developing direction of ruminant green nourishing technology, also improves social ecological benefits simultaneously.In cow concentrate, add the 5%-10% fermentative feedstuff of microbe and can increase the output of milk 10%.In the sheep finishing concentrate, add the 5%-10% fermentative feedstuff of microbe and can improve daily gain 15%.

Description of drawings

Accompanying drawing is this preparation method's a process chart

The specific embodiment

1,10kg molasses hydrolyzate preparation:

Water 8kg, molasses 2kg, phosphoric acid 0.004kg, sulfuric acid 0.0025kg mix fully agitation and dilution of back, and steam boils 30min, and adjust pH is 4.8, filter after leaving standstill 10min, and supernatant is as fermentation culture, and moisturizing becomes the molasses acidifying solution to 10kg.

2, activate and the culture medium that spreads cultivation is formed (g/kg):

A, candida utili

Molasses acidifying solution 200g, ammonium nitrate 5g, potassium dihydrogen phosphate 2.5g, sodium hydrogen phosphate 2.5g.

B, geotrichum candidum

Malt extract medium.

C, streptococcus fecalis

Molasses acidifying solution 100g, yeast extract 3g, potato leachate (200g potato leachate).

3, spread cultivation technology and parameter

A, candida utili zymotic fluid (4kg)

Taking over a job bacterium liquid 400ml among the fermentation medium 4kg, 28-30 ℃, the 6r/min stir culture is cultivated 24h.

B, geotrichum candidum zymotic fluid (4kg)

Taking over a job bacterium liquid 400ml among the basic 4kg of fermentation training, 30 ℃, the 6r/min stir culture is cultivated 24h.

C, streptococcus fecalis zymocyte liquid (2kg)

Fermentation medium PH 6.7, taking over a job bacterium liquid 200ml among the 2kg, 30 ℃, 6r/min stirs, and cultivates 18h.

4, mix

To cultivate back bacterium liquid 4kg candida utili spread cultivation liquid and the 2kg streptococcus fecalis of liquid, 4kg geotrichum candidum that spread cultivation and spread cultivation and mix in the liquid importing blending tank, make the 10kg mixed bacteria liquid.

5, solid formulation fermentation (100kg)

A, solid-state fermentation culture medium are formed

Molasses liquefier 5kg, maize peel 20kg, rice bran 20kg, cottonseed dregs of rice 25kg, wheat bran 15kg, Fructus Hordei Germinatus 15kg form the 100kg solid-state fermentation culture medium.

B, solid-state fermentation process parameter

Add 10kg mixed bacteria liquid and supplementing water 20kg, 28-30 ℃ of condition bottom fermentation 28-48h in the 100kg solid-state fermentation culture medium.

C, fermentation post processing

55-60 ℃ of low temperature drying after the fermentation ends made milk cow (sheep) rumen microbial fermentation feed 100kg after the pulverizing.

In order to guarantee the viable count of product beneficial bacterium, with the wet fermentative feedstuff of microbe that ferments once more with the fermentative feedstuff of microbe of low temperature drying according to 1: 9 weight ratio mixing and stirring after the metering packing be finished product.

Claims

1. the preparation method of a fermentative feedstuff of microbe, it is characterized in that: with molasses as liquid medium starting material to candida utili, geotrichum candidum and streptococcus fecalis enlarge respectively to cultivate and become to spread cultivation liquid, and by the 30%-45% candida utili liquid that spreads cultivation, the 30%-45% geotrichum candidum liquid that spreads cultivation, the spread cultivation weight ratio of liquid of 10%-30% streptococcus fecalis is mixed and made into mixed bacteria liquid, the weight ratio of mixed bacteria liquid being pressed 5%-15% adds by 5% molasses liquefier, 20% maize peel, the 10% dish dregs of rice, the 10% cottonseed dregs of rice, 20% wheat bran, 20% bean dregs, in the semi-solid ferment culture medium that 15% pomace constitutes, under 28-30 ℃ of condition, fermented 24-48 hour, to be lower than 60 ℃ of low temperature dryings, to pulverize again and be fermentative feedstuff of microbe after the fermentation ends.