CN103564199A - Method for producing cow feed by multi-strain mixing solid-state fermentation - Google Patents
Method for producing cow feed by multi-strain mixing solid-state fermentation Download PDFInfo
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- CN103564199A CN103564199A CN201210267918.3A CN201210267918A CN103564199A CN 103564199 A CN103564199 A CN 103564199A CN 201210267918 A CN201210267918 A CN 201210267918A CN 103564199 A CN103564199 A CN 103564199A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to a feed preparation method, and particularly, relates to a method for producing cow feed by multi-strain mixing solid-state fermentation. The method comprises the following steps: preparing a fermentation feed solid medium, mixing dry beer grains, dry corn straw powder, wheat bran, rice bran, soybean meal, urea, and ammonium sulfate according to a ratio of 70:6:6:6:6:4:2, adding water to control the water content of the medium to be 60-65%, well mixing, steaming at normal pressure for 1 hour, cooling to 30 DEG C, respectively inoculating bacillus subtilis seed liquid, candida utilis seed liquid, and saccharomyces cerevisiae seed liquid on the solid medium, well mixing, spreading on a disinfected bamboo curtain, performing fermentation in a thermostat at 30-34 DEG C for 56-60 hours, and drying at 45 DEG C to obtain a water content of less than 10% so as to obtain the finished product. The product of the invention can improve nutrition value of cow feed, increase feed utilization rate, reduce cow incidence of diseases, and increase milk yield.
Description
(1), technical field
That the present invention narrates is a kind of preparation method of feed, and specifically many bacterial classifications mixed solid fermentation is produced the method for milk cow forage.
(2), background technology
Stalk and brewex's grains are common feedstuffses of the ruminants such as milk cow, but these feeds are directly fed milk cow, not only can cause the utilization rate of active components in feed on the low side, long-term edible also can cause that dairy cow nutrition is bad, hypoimmunity, reduce the rate of giving milk of milk cow.
Brewex's grains are main byproducts of brewing industry, and in recent years, the development of the brewing industry of China is swift and violent, and the wet poor annual production of beer reaches more than 8,000,000 tons.For a long time, the brewex's grains of China are main directly as farmers''s feed, because water content is high, are difficult to storage, very easily mildew, and consumption is limited.
China's agricultural crop straw annual production reaches 700,000,000 tons, if all turned waste into wealth, rational exploitation and utilization, can increase farmers' income, and the grain that saving and alternatives is a large amount of can also reduce the discharge of a large amount of greenhouse gases.Concerning animal husbandry, 700,000,000 tons of straw nutritions are worth and are equivalent to nearly 200,000,000 ton-grain foods, can be animal husbandry sustainable development material guarantee is provided.Greatly develop straw fermented feed, be conducive to alleviate the problem of China's protein feed shortage, and can promote the modern agriculture of Agro-ecology balance and construction efficient ecological.
Take brewex's grains as primary raw material, the accessory substance that is aided with the crops processing such as maize straw, wheat bran, rice bran, dregs of beans, adopt many bacterial classifications mixed solid fermentation to produce milk cow forage, not only turn waste into wealth, also greatly improved the raw materials such as brewex's grains nutritive value and utilization rate separately, improve the absorption rate of milk cow to nutriment in feed, under the prerequisite that guarantees milk quality, improved the output of milk.
(3), summary of the invention
The object of the present invention is to provide a kind ofly can increase milk cow forage nutritive value, improves the utilization rate of feed, reduces the incidence of disease of milk cow and the raising method that many bacterial classifications mixed solid fermentation of rate produces milk cow forage of giving milk.
The object of the present invention is achieved like this:
1, the preparation of bacillus subtilis seed liquor
Take 20 grams of moyashi, add 120 milliliters, water, boil 30 minutes, by filtered through gauze, water is supplied filtrate to 120 milliliter, add glucose 6g, after stirring and dissolving, pour in 500ml triangular flask, 121 ℃ of sterilizings 20 minutes, be cooled to 32 ℃, from inclined-plane, inoculate a ring bacillus subtilis, under 30～32 ℃, the condition of rotary shaker 150rpm, cultivate 16～18 hours.
2, the preparation of candida utili seed liquor
Take some fructus hordei germinatus, pulverize, add 4 times of water, under 58～65 ℃ of conditions, saccharification is 3～4 hours, until measuring saccharified liquid, iodine liquid reacts without blueness, by 6 layers of filtered through gauze for saccharified liquid, get 100 milliliters of filtrates, pour in 500ml triangular flask, 121 ℃ of sterilizings 20 minutes, be cooled to 30 ℃, from inclined-plane, inoculate a ring candida utili, under 30～32 ℃, the condition of rotary shaker 150rpm, cultivate 16～18 hours.
3, the preparation of saccharomyces cerevisiae seed liquor
Take 100 grams of corn flours, be placed in 500ml triangular flask, add water 600ml, shake up, ratio in every gram of corn flour 15～20 units adds thermostable α-amylase, under 90～95 ℃ of conditions, is incubated 30 minutes, is cooled to 60 ℃, ratio in every gram of corn flour 100～150 units adds carbohydrase, maintains 58 ℃～60 ℃ saccharification 4 hours.Get 100 milliliters of saccharified liquids, add 1 milliliter of corn steep liquor, 0.5 ammonium sulfate, 0.1 gram of potassium dihydrogen phosphate, 0.05 gram of magnesium sulfate, 121 ℃ of sterilizings 20 minutes, are cooled to 30 ℃, from inclined-plane, inoculate a ring saccharomyces cerevisiae, under 30～32 ℃, the condition of rotary shaker 150rpm, cultivate 16～18 hours.
4, the preparation of milk cow fermented feed
First prepare fermented feed solid medium, by dry beer vinasse, dry corn stalk powder, wheat bran, rice bran, dregs of beans, urea, ammonium sulfate mixes according to the ratio of 70:6:6:6:6:4:2, add water, moisture content in medium is controlled to 60～65%, mix, normal pressure steams 1 hour, be cooled to 30 ℃, by bacillus subtilis seed liquor, candida utili seed liquor, saccharomyces cerevisiae seed liquor is inoculated into respectively in above-mentioned solid medium, inoculum concentration be every kind of bacterial classification each 10%, mix rear tiling to the bamboo curtain splint of sterilizing, 4～6 centimetres of thickness, in 30～34 ℃ of insulating boxs, ferment 56～60 hours, then under 45 ℃ of conditions, be dried to moisture below 10%, get product.
Product of the present invention is containing a large amount of useful viable bacteria body, multivitamin, multiple biology enzyme and amino acid, small peptide, good protein etc., and these nutritional labelings, are all the metabolites in microorganism growth process, is not to add in addition.Therefore can be referred to as the pollution-free micro-ecological ideas feed of pure natural of new generation.This feed has the milk cow of promotion and grows, and improves efficiency of feed utilization, increases immunity, improves milk cow intestinal microecology, prevents disease, improves the quality of milk, improves the output of milk, reduces the multiple advantages such as environmental pollution.
Product of the present invention is a kind of pure natural, without micro-ecological ideas feed of any additive.This product had both met the development trend of international feed industry, the development plan that has more adapted to China's feed industry, strong promotion China's feed industry towards future development healthy, green, environmental protection, for the great development of China's milk cattle cultivating industry and for providing safer milk that Reliable guarantee is provided on common people's dining table, social effect is extremely great.
(4), specific embodiments
The strain purchase that the present invention adopts is in Chinese industrial microorganism fungus kind preservation administrative center, and numbering is respectively bacillus subtilis CICC21789, candida utili CICC32232, saccharomyces cerevisiae CICC1355
1, the preparation of bacillus subtilis seed liquor
Take 20 grams of moyashi, add 120 milliliters, water, boil 30 minutes, by filtered through gauze, water is supplied filtrate to 120 milliliter, add glucose 6g, after stirring and dissolving, pour in 500ml triangular flask, 121 ℃ of sterilizings 20 minutes, be cooled to 32 ℃, from inclined-plane, inoculate a ring bacillus subtilis, under 32 ℃, the condition of rotary shaker 150rpm, cultivate 16 hours.
2, the preparation of candida utili seed liquor
Take 50g fructus hordei germinatus, pulverize, add 200ml water, under 58～65 ℃ of conditions, saccharification is 4 hours, until measuring saccharified liquid, iodine liquid reacts without blueness, by 6 layers of filtered through gauze for saccharified liquid, get 100 milliliters of filtrates, pour in 500ml triangular flask, 121 ℃ of sterilizings 20 minutes, be cooled to 30 ℃, from inclined-plane, inoculate a ring candida utili, under 32 ℃, the condition of rotary shaker 150rpm, cultivate 16 hours.
3, the preparation of saccharomyces cerevisiae seed liquor
Take 100 grams of corn flours, be placed in 500ml triangular flask, add water 600ml, shake up, ratio in every gram of corn flour 15～20 units adds thermostable α-amylase, under 90～95 ℃ of conditions, is incubated 30 minutes, is cooled to 60 ℃, ratio in every gram of corn flour 100～150 units adds carbohydrase, maintains 58 ℃～60 ℃ saccharification 4 hours.Get 100 milliliters of saccharified liquids, add 1 milliliter of corn steep liquor, 0.5 ammonium sulfate, 0.1 gram of potassium dihydrogen phosphate, 0.05 gram of magnesium sulfate, 121 ℃ of sterilizings 20 minutes, are cooled to 30 ℃, from inclined-plane, inoculate a ring saccharomyces cerevisiae, under 32 ℃, the condition of rotary shaker 150rpm, cultivate 16 hours.
4, the preparation of milk cow fermented feed
First prepare fermented feed solid medium, take dry beer vinasse 350g, dry corn stalk powder 30g, wheat bran 30g, rice bran 30g, dregs of beans 30g, urea 20g, ammonium sulfate 10g, add water moisture content in medium is controlled to 65%, mix, normal pressure steams 1 hour, be cooled to 30 ℃, by bacillus subtilis seed liquor, candida utili seed liquor, each 50ml of saccharomyces cerevisiae seed liquor is inoculated into respectively in above-mentioned solid medium, mix rear tiling to the bamboo curtain splint of sterilizing, 4 centimetres of thickness, in 30 ℃ of insulating boxs, ferment 60 hours, then under 45 ℃ of conditions, be dried to moisture below 10%, get product.
Claims (4)
1. more than bacterial classification mixed solid fermentation is produced the method for milk cow forage, it is characterized in that: by dry beer vinasse, dry corn stalk powder, wheat bran, rice bran, dregs of beans, urea, ammonium sulfate mixes according to the ratio of 70:6:6:6:6:4:2, add water, water content is controlled to 60～65%, mix, normal pressure steams 1 hour, be cooled to 30 ℃, by bacillus subtilis seed liquor, candida utili seed liquor, saccharomyces cerevisiae seed liquor is inoculated into respectively in above-mentioned solid medium, inoculum concentration be every kind of bacterial classification each 10%, mix rear tiling to the bamboo curtain splint of sterilizing, 4～6 centimetres of thickness, in 30～34 ℃ of insulating boxs, ferment 56～60 hours, then under 45 ℃ of conditions, be dried to moisture below 10%, get product.
2. many bacterial classifications mixed solid fermentation according to claim 1 is produced the method for milk cow forage, it is characterized in that: the preparation method of described bacillus subtilis seed liquor is: take 20 grams of moyashi, add 120 milliliters, water, boil 30 minutes, by filtered through gauze, water is supplied filtrate to 120 milliliter, add glucose 6g, after stirring and dissolving, pour in 500ml triangular flask, 121 ℃ of sterilizings 20 minutes, are cooled to 32 ℃, from inclined-plane, inoculate a ring bacillus subtilis, under 30～32 ℃, the condition of rotary shaker 150rpm, cultivate 16～18 hours.
3. many bacterial classifications mixed solid fermentation according to claim 1 is produced the method for milk cow forage, it is characterized in that: the preparation method of described candida utili seed liquor is: take some fructus hordei germinatus, pulverize, add 4 times of water, under 58～65 ℃ of conditions, saccharification is 3～4 hours, until measuring saccharified liquid, iodine liquid reacts without blueness, by 6 layers of filtered through gauze for saccharified liquid, get 100 milliliters of filtrates, pour in 500ml triangular flask, 121 ℃ of sterilizings 20 minutes, be cooled to 30 ℃, from inclined-plane, inoculate a ring candida utili, at 30～32 ℃, under the condition of rotary shaker 150rpm, cultivate 16～18 hours.
4. many bacterial classifications mixed solid fermentation according to claim 1 is produced the method for milk cow forage, it is characterized in that: the preparation method of described saccharomyces cerevisiae seed liquor is: take 100 grams of corn flours, be placed in 500ml triangular flask, add water 600ml, shake up, ratio in every gram of corn flour 15～20 units adds thermostable α-amylase, under 90～95 ℃ of conditions, be incubated 30 minutes, be cooled to 60 ℃, ratio in every gram of corn flour 100～150 units adds carbohydrase, maintains 58 ℃～60 ℃ saccharification 4 hours.Get 100 milliliters of saccharified liquids, add 1 milliliter of corn steep liquor, 0.5 ammonium sulfate, 0.1 gram of potassium dihydrogen phosphate, 0.05 gram of magnesium sulfate, 121 ℃ of sterilizings 20 minutes, are cooled to 30 ℃, from inclined-plane, inoculate a ring saccharomyces cerevisiae, under 30～32 ℃, the condition of rotary shaker 150rpm, cultivate 16～18 hours.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104106727A (en) * | 2014-06-12 | 2014-10-22 | 吉林省农业科学院 | Complete fermented feed and preparing method thereof |
| CN104171410A (en) * | 2014-08-03 | 2014-12-03 | 哈尔滨伟平科技开发有限公司 | Making method of milk cow feed additive |
| CN104273378A (en) * | 2014-10-10 | 2015-01-14 | 青岛嘉瑞生物技术有限公司 | New process for producing fermented feed for dairy cows by taking wheat straw and distillers' grains as raw materials |
| CN104489244A (en) * | 2015-01-12 | 2015-04-08 | 赵彦明 | Black microorganism feed and preparation technology thereof |
| CN104782892A (en) * | 2015-04-30 | 2015-07-22 | 陈鹏 | Bagasse fermented feed and preparation method thereof |
| CN104893995A (en) * | 2015-07-09 | 2015-09-09 | 仁怀市城北中学 | Saccharomycetes culture method and observation method for saccharomycetes gemmation |
| CN105076675A (en) * | 2015-08-18 | 2015-11-25 | 河南普爱饲料股份有限公司 | Novel solid state fermentation technology of corn flour with low moisture |
| CN106387380A (en) * | 2016-08-31 | 2017-02-15 | 盐城工学院 | Suaeda salsa fermented livestock feed and preparation method thereof |
| CN106615616A (en) * | 2017-01-05 | 2017-05-10 | 哈尔滨伟平科技开发有限公司 | Method for preparing microbial fermentation type swine feed |
| CN109744393A (en) * | 2019-03-09 | 2019-05-14 | 长沙微威生物科技有限公司 | A kind of fodder additives for ox comprising enzyme preparation and beneficial flora solid state rheology object |
| CN109984261A (en) * | 2017-12-29 | 2019-07-09 | 湖南碧野生物科技有限公司 | A kind of dedicated straw fermented feed of beef cattle and its preparation process |
| CN117461729A (en) * | 2023-12-07 | 2024-01-30 | 宜宾市南溪区国科中农生物科技有限公司 | Fermented biological feed containing vinasse and preparation method thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104106727A (en) * | 2014-06-12 | 2014-10-22 | 吉林省农业科学院 | Complete fermented feed and preparing method thereof |
| CN104171410A (en) * | 2014-08-03 | 2014-12-03 | 哈尔滨伟平科技开发有限公司 | Making method of milk cow feed additive |
| CN104273378A (en) * | 2014-10-10 | 2015-01-14 | 青岛嘉瑞生物技术有限公司 | New process for producing fermented feed for dairy cows by taking wheat straw and distillers' grains as raw materials |
| CN104489244A (en) * | 2015-01-12 | 2015-04-08 | 赵彦明 | Black microorganism feed and preparation technology thereof |
| CN104782892B (en) * | 2015-04-30 | 2018-01-09 | 陈鹏 | A kind of sugarcane residue fermented feed and preparation method thereof |
| CN104782892A (en) * | 2015-04-30 | 2015-07-22 | 陈鹏 | Bagasse fermented feed and preparation method thereof |
| CN104893995A (en) * | 2015-07-09 | 2015-09-09 | 仁怀市城北中学 | Saccharomycetes culture method and observation method for saccharomycetes gemmation |
| CN105076675A (en) * | 2015-08-18 | 2015-11-25 | 河南普爱饲料股份有限公司 | Novel solid state fermentation technology of corn flour with low moisture |
| CN106387380A (en) * | 2016-08-31 | 2017-02-15 | 盐城工学院 | Suaeda salsa fermented livestock feed and preparation method thereof |
| CN106615616A (en) * | 2017-01-05 | 2017-05-10 | 哈尔滨伟平科技开发有限公司 | Method for preparing microbial fermentation type swine feed |
| CN109984261A (en) * | 2017-12-29 | 2019-07-09 | 湖南碧野生物科技有限公司 | A kind of dedicated straw fermented feed of beef cattle and its preparation process |
| CN109744393A (en) * | 2019-03-09 | 2019-05-14 | 长沙微威生物科技有限公司 | A kind of fodder additives for ox comprising enzyme preparation and beneficial flora solid state rheology object |
| CN117461729A (en) * | 2023-12-07 | 2024-01-30 | 宜宾市南溪区国科中农生物科技有限公司 | Fermented biological feed containing vinasse and preparation method thereof |
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Application publication date: 20140212 |