CN109619265A - A kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation - Google Patents
A kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation Download PDFInfo
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- CN109619265A CN109619265A CN201910019314.9A CN201910019314A CN109619265A CN 109619265 A CN109619265 A CN 109619265A CN 201910019314 A CN201910019314 A CN 201910019314A CN 109619265 A CN109619265 A CN 109619265A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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Abstract
The invention discloses a kind of methods of selenium-enriched cordceps militaris edible fungi residue feed preparation.Steps are as follows: (1) recycling of Cordyceps militaris culture residue；(2) yellow meal worm selenium-rich is raised；(3) selenium-rich yellow meal worm raising post-processing；(4) prepared by Chinese caterpillar fungus fermentation feed.I.e. Cordyceps militaris cultivates residue silty amount: selenium-rich yellow meal worm quality (Se content 0.3mg/kg): soya bean Soybean Meal=9:1:1, and the biological feedstuff leavening for adding 100mg/kg carries out feed fermentation.Novel selenium-enriched Chinese caterpillar fungus feed prepared by the present invention is measured through component content, meets pannage national standard content as the result is shown.The waste material of Cordyceps militaris culture residue is applied on feed formulation as major ingredient, Cordyceps militaris active material (cordycepic acid, Cordyceps sinensis polysaccharide etc.) is made to increase；Feed is prepared using selenium-rich yellow meal worm to improve crude protein, crude fibre and Se content, and to achieve the purpose that breeding performonce fo animals improve and immune function enhances, in feed industry, aquaculture field has broad application prospects and economic value.
Description
Technical field
The invention belongs to technique for producing feed fields, and in particular to a kind of to cultivate residue and yellow meal worm with Cordyceps militaris as original
Material, the method for preparing novel selenium-enriched Chinese caterpillar fungus feed.
Background technique
Cordyceps militaris production at this stage mostly uses solid culture mode greatly, after fruiting bodies of cordyceps militaris harvesting, cultivates in residue
It is not fully used containing a large amount of mycelium and nutritional ingredient still, causes the waste of resource.In addition, antibiotic, hormone,
Abuse of the illegal chemical synthetic drug in cultivation, so that cordyceps sinensis class feed comes into being.
Play the role of research data to show: cordyceps sinensis class feed addictive is mainly reflected in the improvement of production performance to pig and exempts from
In terms of the enhancing of epidemic disease function.Aweto fungus fermentation of Chinese herbal medicine culture medium feeds growing and fattening pigs as raw material, can be improved growing and fattening pigs
Daily gain, dressing percentage and lean meat percentage reduce feedstuff-meat ratio and back-fat thickness.And other Chinese caterpillar fungus feed additives to child care piglet and bar
The small-sized daily gain in pigs of horse and feed conversion rate also have positive influence, and have growth-promoting effect to growing and fattening pigs.Selenium is
Nutrient necessary to object, when selenium deficiency, animal body inner cell antiperoxide toxicity ability is reduced, and cell is broken by peroxide
It is bad, there is oedema, bleeding, exudative diathesis, necrosis of liver cells, spleen fibroid atrophy, muscle is abnormal, muscular degeneration of heart.?
Inorganic selenium or selenium-rich yellow meal worm are added in feedstuff, the content that can effectively improve selenium in feed can fundamentally solve animal and lack
Selenium symptom.And the organic selenium in yellow meal worm is safer compared to inorganic selenium (sodium selenite), animal assimilation effect is more preferable.In addition, yellow
Crude protein and crude fibre substance abundant can also be provided in mealworm.
It is the development and utilization and industrialized production of cordyceps militaris series products by cultivating Cordyceps militaris the processing of residue
Technical support is provided.The Chinese caterpillar fungus feed researched and developed, the later period is using techniques such as industrial fermentation technologies, it will realizes the production of cordyceps sinensis production
Industry largely can reduce production cost, will lay the foundation for its scale application in cultivation industry.
Summary of the invention
For in cordyceps sinensis growing industry cultivate residue waste, livestock drug abuse and livestock production performance with exempt from
Situations such as epidemic disease hypofunction, is answered by the waste material resource that Cordyceps militaris is cultivated residue as major ingredient under the background of the prior art
It uses on feed formulation, crude protein, crude fibre and Se content, overcome the deficiencies in the prior art is improved using selenium-rich yellow meal worm.
The present invention provides a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation, comprises the following steps that
(1) recycling of Cordyceps militaris culture residue:
The remaining culture residue in laboratory after the harvesting of culture fruiting bodies of cordyceps militaris is selected, after being dried in 60 DEG C of baking ovens,
It is crushed with pulverizer.
(2) yellow meal worm selenium-rich is raised:
It chooses Yellow meal worm larva (height about 2cm, commercially available), indoor raising, feeds autogamy feed, temperature is about 21-23 DEG C,
Sieve excrement frequency 5~7d/ times is raised 30 days.The internal Se content not exceeded (Se content≤0.5mg/kg) of selection is not poisoned
Yellow meal worm carries out fermented feed preparation.
(3) selenium-rich yellow meal worm raising post-processing:
Take the yellow meal worm of the raising of selenium-rich, hungry 3d, it is quick-frozen it is lethal after, dry, be sealed spare under the conditions of 80 DEG C.
(4) prepared by Chinese caterpillar fungus fermentation feed:
In 90g Cordyceps militaris culture residue, it is added 10g selenium-rich yellow meal worm (Se content 0.3mg/kg), 10g soya bean beans
The dregs of rice, the biological feedstuff leavening of 100mg/kg, raw material moisture ferment for 30% or so, are sealed by fermentation 5-7d, avoid sunlight
Direct projection.
Wherein Chinese caterpillar fungus culture medium raw material proportioning are as follows: potato 200g/L, glucose 20g/L, potassium dihydrogen phosphate 3g/L, sulphur
Sour magnesium 1.5g/L, peptone 5g/L, vitaminB10 .05g/L.30g wheat is added in every 50mL culture medium for cultivating.
Wherein Cordyceps militaris culture residue is culture Cordyceps militaris 35 days or so, the remaining culture after fruiting bodies of cordyceps militaris is harvested
Residue.
Wherein yellow meal worm selenium-rich culturing feed matches are as follows: wheat bran 70%, corn flour 24%, soy meal 5%, milk powder 1%.Three
The different selenium element additive amounts of group are respectively Se1=15mg/kg, Se2=25mg/kg, Se3=30mg/kg.
Wherein the selenium element source of yellow meal worm is mainly the sodium selenite manually added in feed.
Wherein yellow meal worm selenium-rich raising temperature is 21-23 DEG C, and sieve excrement frequency is 5-7d/ times, is cultivated 30 days.
The wherein selenium-rich yellow meal worm of the final concentration of 0.3mg/kg of Se content, according to a certain percentage by different Se content yellow meal worms
It deploys.The yellow meal worm selecting internal Se content not exceeded (Se content Wei≤0.5mg/kg) when allotment, not being poisoned.
Wherein feed making, which specifically matches, is；Cordyceps militaris cultivates residue 90g, and soya bean dregs of beans 10g, (selenium contains selenium-rich yellow meal worm
Measure final concentration of 0.3mg/kg) 10g, biological feedstuff leavening 100mg/kg, moisture is 30% or so.
Wherein biological feedstuff leavening comes from Zhengzhou Le Beifeng Biotechnology Co., Ltd.
Wherein Chinese caterpillar fungus feed meets by the measurement of the component contents such as selenium, crude protein, crude fibre, total phosphorus, copper, moisture
Pannage national standard content, and a large amount of Cordyceps militaris active material (cordycepic acid, Cordyceps sinensis polysaccharide) obtains the nutritional ingredient of feed
To improve.
Measurement is optimized to five kinds of different Cordyceps militaris culture residues and soya bean dregs of beans proportion, be respectively cultivate residue 60g,
Dregs of beans 40g cultivates residue 70g, dregs of beans 30g, cultivates residue 80g, dregs of beans 20g, cultivates residue 90g, dregs of beans 10g, cultivates residue
100g, dregs of beans 0g.After measured in prepared feed when Cordyceps militaris culture residue amount it is more, Cordyceps sinensis polysaccharide and cordyceps sinensis in feed
Acid content is higher, and crude protein content is lower.When not adding soya bean dregs of beans in feed, the crude protein content of feed does not meet state
Family's standard.With selenium, Cordyceps sinensis polysaccharide, cordycepic acid and crude protein content, pH value etc. in feed for index, Cordyceps militaris in feed can be obtained
The optimum proportioning of culture residue and soya bean dregs of beans is culture residue 90g, dregs of beans 10g.
The present invention is using yellow meal worm as one of auxiliary material, and advantage is first is that be enriched with selenium element as carrier using yellow meal worm, by nothing
Machine selenium is converted into organic selenium, and more conducively absorption of the animal to selenium element, yield reduces caused by reducing because of selenium deficiency, fertility declines, product
The phenomenon that matter declines.Advantage is second is that yellow meal worm contains more excellent animal protein, and nutritive value is high, rich amino acids
It is complete, and be conducive to animal and absorb, it is high protein nutrient enriched dose ideal.In addition, yellow meal worm also has unsaturated lipid abundant
Fat acid, microelement and vitamin, are a kind of high-nutrition food raw materials.
The present invention re-uses the waste material culture medium containing remaining nutriment, reduces the waste of resource.In addition, culture
Remaining cordycepic acid, Cordyceps sinensis polysaccharide, cordycepin are that animal growth plays important work as Cordyceps militaris active material in base
With.Selenium, crude protein, crude fibre, total phosphorus, copper, moisture content meet pannage national standard in the obtained feed of the present invention
Content, and a certain amount of Cordyceps militaris active material (cordycepic acid, Cordyceps sinensis polysaccharide) is remained in feed, obtain the nutritional ingredient of feed
To improve.
Specific embodiment
Embodiment is in detail below convenient for being best understood from the present invention, but does not limit the present invention.Unless otherwise specified, of the invention
Used in reagent, material be conventional reagent, material.Unless otherwise specified, used method is conventional method.The present invention
It is substantially carried out the recycling, the raising of yellow meal worm selenium-rich, selenium-rich yellow meal worm raising post-processing, Chinese caterpillar fungus fermentation feed of Cordyceps militaris culture residue
Prepare this four big step.
Cordyceps militaris spawn slant medium: potato 200g/L, agar powder 16g/L, triammonium citrate 1g/L, biphosphate
Potassium 2g/L, magnesium sulfate 1g/L, glucose 2g/L, peptone 5g/L, vitaminB10 .05g/L.
Cordyceps militaris spawn liquid activation medium: potato 200g/L, peptone 5g/L, glucose 20g/L, citric acid three
Amine 1g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, vitaminB10 .05g/L.
Cordyceps militaris spawn culture medium for cultivating: potato 200g/L, glucose 20g/L, potassium dihydrogen phosphate 3g/L, magnesium sulfate
1.5g/L, peptone 5g/L, vitaminB10 .05g/L.30g wheat, 110 DEG C of high pressure sterilizations are added in every 25mL culture medium for cultivating
3h。
Cordyceps militaris cultivates residue: after the fruiting bodies of cordyceps militaris harvesting of laboratory cultures, remaining culture residue.
Selenium-rich yellow meal worm feed: wheat bran 70%, corn flour 24%, soy meal 5%, milk powder 1%, sodium selenite.Wherein set
Three groups do not have to selenium element additive amount, respectively Se1=15mg/kg, Se2=25mg/kg, Se3=30mg/kg, by sodium selenite
It is soluble in water, it is stirred evenly with feed.
Chinese caterpillar fungus feed: Cordyceps militaris cultivates residue 90g, selenium-rich yellow meal worm (Se content 0.3mg/kg) 10g, soya bean dregs of beans
10g, moisture are about 30%, biological feedstuff leavening 100mg/kg.
Biological feedstuff leavening: Zhengzhou Le Beifeng Biotechnology Co., Ltd.
Specific embodiment step 1: the recycling of Cordyceps militaris culture residue
1, cordyceps militaris plantation method: using strain to save strain for laboratory, cultivates according to conventional cultivation method, that is, includes
Several big steps such as actication of culture, inoculation, culture should be noted management in incubation, and stroma growth phase need to use syringe needle after annesl
Every bottle closure film is pricked into 3-5 hole, cultivation temperature is 22 DEG C, humidity 80-90%, illumination 12h/d, and intensity of illumination is
200lx divulges information as 2h/d, until stroma no longer extends, about 35d or so.After stroma top is no longer expanded, it can be adopted
It receives, when harvesting does not break stroma.
2, the acquisition of Cordyceps militaris culture residue: the Cordyceps militaris after selection harvesting cultivates residue, after being dried in 60 DEG C of baking ovens,
It is crushed with pulverizer.
Specific embodiment step 2: the raising of selenium-rich yellow meal worm
Yellow meal worm larva (height about 2cm, commercially available) is chosen, autogamy feed is fed, is raised indoors, temperature is about
It 21-23 DEG C, sieves excrement frequency 5~7d/ times, raises 30 days.Sodium selenite is soluble in water, it is stirred evenly with feed.If three groups are not
It is raised with selenium element additive amount, to obtain the different yellow meal worm of Se content, to deploy to purpose Se content.
Specific embodiment step 3: selenium-rich yellow meal worm raising post-processing
By selenium-rich raising 30d yellow meal worm, hungry 3d, it is quick-frozen it is lethal after, dry, be sealed standby under the conditions of 80 DEG C
With and measuring its selenium-rich amount, obtain the different yellow meal worm of Se content, (purpose Se content is to purpose Se content to deploy
0.3mg/kg)。
Specific embodiment step 4: the preparation of Chinese caterpillar fungus fermentation feed
In 90g Cordyceps militaris culture residue, it is added 10g selenium-rich yellow meal worm (Se content 0.3mg/kg), 10g soya bean beans
The dregs of rice, the biological feedstuff leavening of 100mg/kg, raw material moisture ferment for 30% or so.It is sealed by fermentation 5-7d, avoids sunlight
Chinese caterpillar fungus feed is made in direct projection.
1 each component content of selenium-enriched cordceps militaris edible fungi residue feed of table
Wherein Chinese caterpillar fungus feed meets by the measurement of the component contents such as selenium, crude protein, crude fibre, total phosphorus, copper, moisture
Pannage national standard content, and a large amount of Cordyceps militaris active material (cordycepic acid, Cordyceps sinensis polysaccharide) obtains the nutritional ingredient of feed
To improve.
Claims (8)
1. a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation, it is characterised in that this method sequentially includes the following steps:
(1) recycling of Cordyceps militaris culture residue；
(2) yellow meal worm selenium-rich is raised；
(3) selenium-rich yellow meal worm raising post-processing；
(4) prepared by Chinese caterpillar fungus fermentation feed: Cordyceps militaris cultivates residue silty amount: selenium-rich yellow meal worm quality (Se content 0.3mg/
Kg): soya bean Soybean Meal=9:1:1, the biological feedstuff leavening for adding 100mg/kg carry out feed fermentation.
2. a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation according to claim 1, it is characterised in that in step (1)
It is selected when the described Cordyceps militaris culture residue recycling and has harvested cordyceps sinensis, no mildew, dry mushroom bran, and with pulverizer powder
It is broken into powder.
3. a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation according to claim 1, it is characterised in that in step (2)
The yellow meal worm feed formula: wheat bran 70%, corn flour 24%, soy meal 5%, milk powder 1%, sodium selenite set three groups of concentration
Configuration.
4. a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation according to claim 1, it is characterised in that in step (2)
If three groups of difference selenium element additive amounts carry out raising yellow meal worm, respectively Se1=15mg/kg, Se2=25mg/kg, Se3=
30mg/kg.(note: Se represents the additive amount of selenium element).
5. a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation according to claim 1, it is characterised in that in step (3)
The yellow meal worm of 30d will be raised, hungry 3d is quick-frozen lethal, under the conditions of 80 DEG C dry after, be sealed it is spare, and measure its richness
Selenium amount.
6. a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation according to claim 1, it is characterised in that in step (3)
The selenium-rich yellow meal worm raised need to carry out selenium-rich measurement and determine, and be deployed according to a certain percentage by different Se content yellow meal worms to Se content
0.3mg/kg, the yellow meal worm that when allotment selects internal Se content not exceeded (Se content Wei≤0.5mg/kg), is not poisoned.
7. a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation according to claim 1, it is characterised in that step (4) worm
Selenium source described in careless fermented feed preparation is mainly the yellow meal worm (Se content 0.3mg/kg) after selenium-rich raising.
8. a kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation according to claim 1, it is characterised in that step (4) worm
Raw material moisture is maintained at 30% or so before fermenting described in careless fermented feed preparation, is sealed by fermentation 5-7d.
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Cited By (3)
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| CN110999867A (en) * | 2019-12-09 | 2020-04-14 | 浙江金宝生物科技有限公司 | Selenium-rich yellow mealworm circulating culture system |
| CN111567689A (en) * | 2020-05-18 | 2020-08-25 | 西北农林科技大学 | Pig feed for improving intestinal health and preparation method thereof |
| CN114451490A (en) * | 2022-02-22 | 2022-05-10 | 沈阳农业大学 | Application of cordyceps militaris residue in improvement of quality of alfalfa silage |
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Application publication date: 20190416 |