CN109924360A - One boar fermentation compound feed and preparation method thereof - Google Patents
One boar fermentation compound feed and preparation method thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention provides boar fermentation compound feed and preparation method thereof, is related to a kind of fermented feed technical field.The invention comprising following raw materials by being prepared: basal feed, aspergillus metabolin, complex enzyme formulation and microbial fermentation inoculum.The invention the preparation method comprises the following steps: in basal feed be added material quality 1 ‰ complex enzyme formulation, then after mixing by raw material, microbial fermentation inoculum, molasses and water, 35-37 DEG C of constant-temperatureanaerobic anaerobic fermentation 24-36h, when material pH value drops to 4.3 or so, low temperature environment is transferred the material into immediately, obtains finished product after detection is qualified.The present invention each stage pig feed digestion utilization rate angle after improving wean, by reasonably adjusting Diet Formula, specific aim selects fermenting microbe and complex enzyme, strict control zymotechnique, reach and improves fermentation compound feed palatability, digestive utilization ratio, energy loss is reduced, the purpose of animal intestinal tract health and the speed of growth is improved.
Description
Technical field
The present invention relates to a kind of fermented feed technical fields, more particularly to a boar fermentation compound feed and its preparation side
Method.
Background technique
Fermented feed is based on Animal nutrition needs and microbial reproduction, to improve efficiency of feed utilization, obtain activity
For the purpose of beneficial bacterium and metabolite, using one or more single feedstuffs as substrate, using single or multi-strain fermentation it is theoretical into
Row reasonable formula, by modern biotechnology, Optimal Parameters and control condition carry out appropriate solid state fermentation processing, and energy
A kind of novel fodder of large-scale production.
Mixed feed refers to that nutritional ingredient is complete, can be directly used in feeding raising object, can meet feeding animals comprehensively
The complete feed of various nutritional needs, it can be directly used in feeding raising object, can meet comprehensively outside feeding object moisture removal
Nutritional need.Fermentation compound feed can remarkably promote the digestibility of feed, and the general level of the health and body for improving animal are exempted from
Epidemic disease disease resistance improves breeding environment, reduces feed cost, has very wide application value.
Although China is large agricultural country, but people and animals strive grain and get worse.At present internal feed protein raw materials about 80% according to
Rely import, and aquaculture cost is high, has lost competitive advantage compared with foreign countries.Farming and animal husbandry is that raising production efficiency is long
Phase largely uses the exogenous growth-promoting agent such as antibiotic, fertilizer and pesticide, diseases prevention agent, leads to the food such as antibiotic residue, pesticide in recent years
Product security incident takes place frequently.
Therefore, safe and reliable novel antimicrobial-free feed is found, is one for ensureing the sustainable development of China's farming and animal husbandry
Effective way.
Summary of the invention
Aiming at the shortcomings existing in the above problems, the present invention provides a boar fermentation compound feed and its preparation side
Method improves the palatability and biocidal property of fermentation compound feed, and General Promotion fermented feed quality improves breeding environment, solves hair
The big problem of ferment mixed feed energy loss, meanwhile, it is good to provide one kind easy to operate, process stabilizing, product using effect
Pig fermentation compound feed preparation method.
To solve the above-mentioned problems, the present invention provides a boar fermentation compound feed, wherein by preparing comprising following raw materials
It forms: basal feed, complex enzyme formulation, microbial fermentation inoculum, molasses and water；Corn 50-55% in the basal feed, greatly
Wheat 5-10%, dregs of beans 20-25%, rice bran meal 5-10%, Cottonseed Meal 5-8%, peanut meal 2-5%, wheat bran 5-10%, plant hydrogen
Calcium 0.5-1%, the microbial fermentation inoculum include lactobacillus plantarum 3-6g, Lactobacillus casei 2-4g, Lactobacillus helveticus 2-5g,
S. cervisiae 5-10g adds beneficial powder 980-1000g；The complex enzyme formulation includes middle acid protease 200-400g, carbohydrase
40-60g, mesophilicα-diastase 50-80g, zytase 10-50g, mannase 0-20g, cellulase 5-25g.
Preferably, by being prepared comprising following raw materials: basal feed, complex enzyme formulation, microbial fermentation inoculum, molasses
And water；Corn 50% in the basal feed, barley 5%, dregs of beans 20%, rice bran meal 5%, Cottonseed Meal 5%, peanut meal 2% are small
Wheat bran 5%, plant hydrogen calcium 0.5%, the microbial fermentation inoculum include lactobacillus plantarum 3g, Lactobacillus casei 2g, Switzerland's cream
Bacillus 2g, S. cervisiae 5g add beneficial powder 980g；The complex enzyme formulation includes middle acid protease 200g, carbohydrase 40g,
Mesophilicα-diastase 50g, zytase 10g, cellulase 5g.
Preferably, by being prepared comprising following raw materials: basal feed, complex enzyme formulation, microbial fermentation inoculum, molasses
And water；Corn 55% in the basal feed, barley 10%, dregs of beans 25%, rice bran meal 10%, Cottonseed Meal 8%, peanut meal 5%,
Wheat bran 10%, plant hydrogen calcium 1%, the microbial fermentation inoculum include lactobacillus plantarum 6g, Lactobacillus casei 4g, Switzerland's cream
Bacillus 5g, S. cervisiae 10g add beneficial powder 1000g；The complex enzyme formulation includes middle acid protease 400g, carbohydrase
60g, mesophilicα-diastase 80g, zytase 50g, mannase 20g, cellulase 25g.
Preferably, the microbial fermentation inoculum is formulated in proportion by following single bacterium: 200,000,000,000/g of lactobacillus plantarum,
200,000,000,000/g of Lactobacillus casei, 100,000,000,000/g of Lactobacillus helveticus, 20,000,000,000/g of S. cervisiae, content of lactic acid bacteria >=10 after compounding
Hundred million/g, yeast bacterial content >=100,000,000/g, total viable count >=1,000,000,000/g, moisture≤10%；The microbial fermentation inoculum additive amount is
Raw material 1kg per ton, carrier are to add beneficial powder；The complex enzyme formulation by being formulated in proportion with lower single enzyme: acid protease
100000U/g, carbohydrase 100000U/g, mesophilicα-diastase 10000U/g, zytase 50000U/g, mannase
10000U/g, cellulase 50000U/g, acid protease >=10000U/g after compounding, carbohydrase >=5000U/g, medium temperature α-shallow lake
Powder enzyme >=100U/g, zytase >=500U/g, cellulase >=200U/g, moisture≤12%, the complex enzyme formulation carrier
For glucose: the water additive amount is raw material 430-500kg per ton, and molasses additive amount is raw material 10-15kg per ton.
The preparation method of one boar fermentation compound feed, wherein the following steps are included:
S10, it is pulverized and mixed: basal feed is crushed, the complex enzyme system of material quality 1 ‰ is added in raw material after being pulverized
Agent is uniformly mixed；
S20, mixing: basal feed, microbial fermentation inoculum, molasses mixture in addition water carry out it is uniformly mixed,
Mixed material moisture is 39-42%；
S30, temperature controlled fermentation: 35-37 DEG C of anaerobic fermentation 24-36h of temperature control first, when material pH value drops to 4.3, transfer
Material is terminated to low temperature environment to ferment；
S40, detection: pig fermentation compound feed is made after the detection of conventional physical and chemical index is qualified in fermentation ends.
Preferably, the constant temperature sterile storage room that the low temperature environment is 2-5 DEG C.
Compared with prior art, the invention has the following advantages that
(1) addition Lactobacillus helveticus is as fermenting agent in the present invention, and compared with other lactic acid bacterias, Lactobacillus helveticus has
Stronger proteolytic activity, it is strong to acid, the tolerance of cholate, promote body to microelement and mineral absorption, was metabolized
Generating in journey has enhancing human metabolism, promotes immune, hormone, enzyme inhibitor, antibacterial, is antiviral and effect for reducing blood fat
Biologically active peptide；
(2) when material pH value drops to 4.3 or so, low temperature environment is transferred the material into immediately and terminates fermentation, to prevent material
Continue to ferment, causes material pH value too low, influence palatability；
(3) the outer additional addition of formula, it is possible to reduce the use of antibiotic has saved cost, improved breeding income；
(4) boar fermentation compound feed viable bacteria colonization ability prepared by the present invention is strong, digestibility is high, enhancement of environment
Effect is obvious, can be used as a kind of substitute of antibiotic, has the stronger market competitiveness.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is made below with reference to example
It is further described, but example is not as a limitation of the invention.
Embodiment 1
(1) basal feed formula: corn 50%, barley 5%, dregs of beans 20%, rice bran meal 5%, Cottonseed Meal 5%, peanut meal
2%, wheat bran 5%, plant hydrogen calcium 1%；
(2) prepared by microbial fermentation inoculum: lactobacillus plantarum 3g, Lactobacillus casei 2g, Lactobacillus helveticus 2g, saccharomyces cerevisiae
Bacterium 5g adds beneficial powder 988g；
(3) prepared by complex enzyme formulation: middle acid protease 200g, carbohydrase 40g, mesophilicα-diastase 50g, zytase
10g, mannase 5g, cellulase 5g, glucose 690g；
(4) raw material in formula is crushed according to reasonable disintegrating process, then by smashed raw material and raw material
The complex enzyme formulation of quality 1 ‰ is uniformly mixed；
(5) raw material, 1 ‰ microbial fermentation inoculums, molasses and water are mixed, water additive amount is raw material 430kg per ton, molasses
Additive amount is raw material 10kg per ton, and the moisture of material is 39.3% after mixing；
(6) 35~37 DEG C of constant-temperatureanaerobic anaerobic fermentation 24-36h immediately shift material when material pH value drops to 4.3 or so
It terminates and ferments to low temperature environment (2-5 DEG C of constant temperature sterile storage room)；
(7) pig fermentation compound feed is made after the detection of conventional physical and chemical index is qualified in fermentation ends, and index is crude protein
15.5%, crude fibre 9.5%, coarse ash 4.8%, calcium 0.45%, total phosphorus 0.17%, the molten protein 12 .4% of acid, total acid 4.24%,
Lactic acid 3.76%, pH4.27,11.1 hundred million/g of content of lactic acid bacteria, yeast bacterial content 2.1 × 106/ g, aflatoxin 9.2ppb, vomits
Spit toxin 276ppb, zearalenone 14.1ppb.
Embodiment 2
(1) basal feed formula: corn 50%, barley 8%, dregs of beans 25%, rice bran meal 5%, Cottonseed Meal 5%, peanut meal
3%, wheat bran 9%, plant hydrogen calcium 1%；
(2) prepared by microbial fermentation inoculum: lactobacillus plantarum 4g, Lactobacillus casei 2g, Lactobacillus helveticus 3g, saccharomyces cerevisiae
Bacterium 10g adds beneficial powder 981g；
(3) prepared by complex enzyme formulation: middle acid protease 300g, carbohydrase 50g, mesophilicα-diastase 60g, zytase
30g, mannase 10g, cellulase 20g, glucose 530g；
(4) raw material in formula is crushed according to reasonable disintegrating process, then by smashed raw material and raw material
The complex enzyme formulation of quality 1.5 ‰ is uniformly mixed；
(5) raw material, 1 ‰ microbial fermentation inoculums, molasses and water are mixed, water additive amount is raw material 460kg per ton, molasses
Additive amount is raw material 10kg per ton, and the moisture of material is 40.4% after mixing；
(6) 35~37 DEG C of constant-temperatureanaerobic anaerobic fermentation 24-36h immediately shift material when material pH value drops to 4.3 or so
It terminates and ferments to low temperature environment (2-5 DEG C of constant temperature sterile storage room)；
(7) pig fermentation compound feed is made after the detection of conventional physical and chemical index is qualified in fermentation ends, and index is crude protein
16.5%, crude fibre 8.7%, coarse ash 4.5%, calcium 0.42%, total phosphorus 0.14%, the molten protein 14 .4% of acid, total acid 4.62%,
Lactic acid 3.91%, pH4.23,10.7 hundred million/g of content of lactic acid bacteria, yeast bacterial content 1.3 × 106/ g, aflatoxin 8.8ppb, vomits
Spit toxin 241ppb, zearalenone 13.7ppb.
Embodiment 3
(1) basal feed formula: corn 55%, barley 10%, dregs of beans 25%, rice bran meal 10%, Cottonseed Meal 8%, peanut
The dregs of rice 5%, wheat bran 10%, plant hydrogen calcium 1%；
(2) prepared by microbial fermentation inoculum: lactobacillus plantarum 6g, Lactobacillus casei 4g, Lactobacillus helveticus 5g, saccharomyces cerevisiae
Bacterium 10g adds beneficial powder 975g；
(3) prepared by complex enzyme formulation: middle acid protease 400g, carbohydrase 60g, mesophilicα-diastase 80g, zytase
50g, mannase 20g, cellulase 25g, glucose 365g；
(4) raw material in formula is crushed according to reasonable disintegrating process, then by smashed raw material and raw material
The complex enzyme formulation of quality 2 ‰ is uniformly mixed；
(5) raw material, 1 ‰ microbial fermentation inoculums, molasses and water are mixed, water additive amount is raw material 500kg per ton, molasses
Additive amount is raw material 15kg per ton, and the moisture of material is 41.7% after mixing；
(6) 35~37 DEG C of constant-temperatureanaerobic anaerobic fermentation 24-36h immediately shift material when material pH value drops to 4.3 or so
It terminates and ferments to low temperature environment (2-5 DEG C of constant temperature sterile storage room)；
(7) pig fermentation compound feed is made after the detection of conventional physical and chemical index is qualified in fermentation ends, and index is crude protein
15.8%, crude fibre 8.5%, coarse ash 4.7%, calcium 0.61%, total phosphorus 0.12%, the molten protein 15 .4% of acid, total acid 4.8%,
Lactic acid 3.92%, pH4.21,12.8 hundred million/g of content of lactic acid bacteria, yeast bacterial content 3.1 × 106/ g, aflatoxin 7.2ppb, vomits
Spit toxin 187ppb, zearalenone 12.4ppb.
For the cultivation application effect of fermentation compound feed prepared by the verifying present invention, applicant is in bohr Lay spy's agriculture and animal husbandry industry
Co., Ltd pig farm chooses 96 average weight 9kg or so " Duroc × length white × great Bai " and hybridizes weanling pig, is randomly divided into 4
A processing, 3 repetitions of each processing, 8 piglets of each repetition.Control group fed Basic drawing, test group additionally feed 10%
The test diet that no-antibiotics feed (1,2,3 group of embodiment) is prepared, feeding time 30d study the fermentation compound feed to disconnected
The influence of milk piglet growth performance.Table 1 is influence of the fermentation compound feed to Growth Performance of Weaning Piglets.
Influence of 1 fermentation compound feed of table to Growth Performance of Weaning Piglets.
Note: data are mean+SD in table, and colleague's subscript indicates significant difference (P < 0.05) containing different letters,
Similarly hereinafter
By 1 test result of table it is found that wean can be significantly improved by additionally adding 10% fermentation compound feed in Basic drawing
Piglet daily gain, daily ingestion amount reduce feed-weight ratio and diarrhea rate.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (6)
1. a boar fermentation compound feed, which is characterized in that by being prepared comprising following raw materials: basal feed, complex enzyme system
Agent, microbial fermentation inoculum, molasses and water；Corn 50-55% in the basal feed, barley 5-10%, dregs of beans 20-25%,
Rice bran meal 5-10%, Cottonseed Meal 5-8%, peanut meal 2-5%, wheat bran 5-10%, plant hydrogen calcium 0.5-1%, the microorganism
Fermenting agent includes lactobacillus plantarum 3-6g, Lactobacillus casei 2-4g, Lactobacillus helveticus 2-5g, and S. cervisiae 5-10g adds benefit
Powder 980-1000g；The complex enzyme formulation includes middle acid protease 200-400g, carbohydrase 40-60g, mesophilicα-diastase
50-80g, zytase 10-50g, mannase 0-20g, cellulase 5-25g.
2. boar fermentation compound feed as described in claim 1, which is characterized in that by being prepared comprising following raw materials:
Basal feed, complex enzyme formulation, microbial fermentation inoculum, molasses and water；Corn 50% in the basal feed, barley 5%, beans
The dregs of rice 20%, rice bran meal 5%, Cottonseed Meal 5%, peanut meal 2%, wheat bran 5%, plant hydrogen calcium 0.5%, the microbial fermentation bacterium
Agent includes lactobacillus plantarum 3g, Lactobacillus casei 2g, Lactobacillus helveticus 2g, S. cervisiae 5g, adds beneficial powder 980g；It is described compound
Enzyme preparation includes middle acid protease 200g, carbohydrase 40g, mesophilicα-diastase 50g, zytase 10g, cellulase 5g.
3. boar fermentation compound feed as described in claim 1, which is characterized in that by being prepared comprising following raw materials:
Basal feed, complex enzyme formulation, microbial fermentation inoculum, molasses and water；Corn 55% in the basal feed, barley 10%,
Dregs of beans 25%, rice bran meal 10%, Cottonseed Meal 8%, peanut meal 5%, wheat bran 10%, plant hydrogen calcium 1%, the microbial fermentation
Microbial inoculum includes lactobacillus plantarum 6g, Lactobacillus casei 4g, Lactobacillus helveticus 5g, S. cervisiae 10g, adds beneficial powder 1000g；It is described
Complex enzyme formulation includes middle acid protease 400g, carbohydrase 60g, mesophilicα-diastase 80g, zytase 50g, mannosan
Enzyme 20g, cellulase 25g.
4. boar fermentation compound feed as described in claim 1, which is characterized in that the microbial fermentation inoculum is by following
Single bacterium is formulated in proportion: 200,000,000,000/g of lactobacillus plantarum, 200,000,000,000/g of Lactobacillus casei, 100,000,000,000/g of Lactobacillus helveticus,
20,000,000,000/g of S. cervisiae, content of lactic acid bacteria >=1,000,000,000/g after compounding, yeast bacterial content >=100,000,000/g, total viable count >=1,000,000,000/
G, moisture≤10%；The microbial fermentation inoculum additive amount is raw material 1kg per ton, and carrier is to add beneficial powder；The complex enzyme system
Agent by being formulated in proportion with lower single enzyme: acid protease 100000U/g, carbohydrase 100000U/g, mesophilicα-diastase
10000U/g, zytase 50000U/g, mannase 10000U/g, cellulase 50000U/g, acidic protein after compounding
Enzyme >=10000U/g, carbohydrase >=5000U/g, mesophilicα-diastase >=100U/g, zytase >=500U/g, cellulase >=
200U/g, moisture≤12%, the complex enzyme formulation carrier are glucose；The water additive amount is raw material 430-500kg per ton,
Molasses additive amount is raw material 10-15kg per ton.
5. the preparation method of a boar fermentation compound feed, which comprises the following steps:
S10, it is pulverized and mixed: basal feed is crushed, the complex enzyme formulation that material quality 1 ‰ is added in raw material after being pulverized is mixed
It closes uniform；
S20, mixing: basal feed, microbial fermentation inoculum, molasses mixture in addition water carry out it is uniformly mixed, mixing
Material moisture afterwards is 39-42%；
S30, temperature controlled fermentation: 35-37 DEG C of anaerobic fermentation 24-36h of temperature control first, when material pH value drops to 4.3, handling material is extremely
Low temperature environment terminates fermentation；
S40, detection: pig fermentation compound feed is made after the detection of conventional physical and chemical index is qualified in fermentation ends.
6. the preparation method of boar fermentation compound feed as claimed in claim 5, which is characterized in that the low temperature environment
For 2-5 DEG C of constant temperature sterile storage room.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110628747A (en) * | 2019-08-30 | 2019-12-31 | 广东溢多利生物科技股份有限公司 | Combined amylase and application thereof |
| CN111109465A (en) * | 2020-01-18 | 2020-05-08 | 成都康菲大地饲料有限公司 | Biological feed for pigs and preparation method thereof |
| CN113088468A (en) * | 2021-04-09 | 2021-07-09 | 中国农业大学 | Lactobacillus casei Ma. GLRGJ1 and application thereof |
| CN114403285A (en) * | 2021-12-29 | 2022-04-29 | 宜昌安琪生物农业科技有限公司 | Feed fermented peanut meal capable of improving protein digestibility, fermentation microbial inoculum and preparation method and application |
| CN117461720A (en) * | 2023-12-05 | 2024-01-30 | 重庆市畜牧科学院 | Complete feed for improving performance of Rong Chang and about gram summer hybrid pigs and application |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628747A (en) * | 2019-08-30 | 2019-12-31 | 广东溢多利生物科技股份有限公司 | Combined amylase and application thereof |
| CN111109465A (en) * | 2020-01-18 | 2020-05-08 | 成都康菲大地饲料有限公司 | Biological feed for pigs and preparation method thereof |
| CN113088468A (en) * | 2021-04-09 | 2021-07-09 | 中国农业大学 | Lactobacillus casei Ma. GLRGJ1 and application thereof |
| CN113088468B (en) * | 2021-04-09 | 2022-06-03 | 中国农业大学 | Lactobacillus casei Ma. GLRGJ1 and application thereof |
| CN114403285A (en) * | 2021-12-29 | 2022-04-29 | 宜昌安琪生物农业科技有限公司 | Feed fermented peanut meal capable of improving protein digestibility, fermentation microbial inoculum and preparation method and application |
| CN117461720A (en) * | 2023-12-05 | 2024-01-30 | 重庆市畜牧科学院 | Complete feed for improving performance of Rong Chang and about gram summer hybrid pigs and application |
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Application publication date: 20190625 |