CN105410410A - Pig complete fermented feed and preparation method thereof - Google Patents
Pig complete fermented feed and preparation method thereof Download PDFInfo
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- CN105410410A CN105410410A CN201511000527.5A CN201511000527A CN105410410A CN 105410410 A CN105410410 A CN 105410410A CN 201511000527 A CN201511000527 A CN 201511000527A CN 105410410 A CN105410410 A CN 105410410A
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Abstract
The invention discloses a pig complete fermented feed preparation method and an application thereof. A pig complete fermented feed comprises the following main components: 60-70.5% of corn, 13-27% of soybean meal and the balance of wheat bran. The strain is composed of mixed lactic acid bacteria, saccharomyces and bacillus according to a ratio of (2-3):(1):(1). The fermented feed is prepared by performing solid state fermentation on a low-nutrition complete feed, molasses and multiple strains, can be used for feeding and nursing, pigs at multiple phases such as growth and pregnancy. The feed combines multi-phase physiological characteristics of pigs and aims at problems of component, nutrition and production of the pig feed at a current specific phase, the pig complete fermented feed can be prepared by using combination fermentation of the specific strains, nutrition value of the feed with low cost is increased, mycotoxin and antinutritional factors are reduced, usage of veterinary drug is reduced, culture cost is reduced, and animal health and production performance can be increased.
Description
Technical field
The present invention relates to the preparation of boar full price fermented feed and an application thereof, industrial by-products molasses and composite bacteria fermentation is utilized to improve feed nutritive value at a low price in particular to one, reduce mycotoxin content, efficient nonreactive ferment complete feed easy to use, belongs to pig feed technical field.
Background technology
Animal is taken in mycotoxin and there will be production performance decline, functional disturbances of gastrointestinal tract, body oxidative damage, immunosupress and premunition and the poisonous effect such as to weaken.And at present China's feedstuff mycotoxin contamination phenomenon is very general, particularly in pig feed, account for the corn of most ratio, toxin recall rate and exceed standard common especially; Also have that wheat and wheat bran vomitoxin pollute, the zearalenone of the aflatoxin contamination of dregs of rice class, corn byproducts pollutes, therefore based on the complete diet pellet of these raw materials often by multiple mycotoxin contamination.Due to feedstuff critical shortage, expensive, and there is the mistake in understanding that growing-finishing pig resistance is strong, can reduce feed quality " rough foster ", so often there is the problems such as raw material is inferior, process is not meticulous, be of low nutritive value, ANFs is many, produce coarse easy cross pollution, mycotoxin content is high in current domestic growing and fattening pig feed.In-pig desired nutritional level is relatively low, and its feed formula structure composition, required technical merit are simple, often out in the cold, thus the in-pig complete diet pellet problem that also easily occurs mycotoxin contamination, be of low nutritive value.Raw material is the basis of digestion, the source of nutrition, and nutritional sufficiency is the basis of animal health, growth and production, nutritional deficiency, and production performance declines naturally thereupon, animal also often occurs disease.At home, the reply means that people commonly use add in feed in violated antibiotic, feeding process to use veterinary drug in a large number, thus easily occurs medicament residue in pork product.And improving constantly along with people's living standard, the attention of people to food quality and safety is more and more higher.Therefore, increase the utilization of unconventional raw material, reduce costs, improve feed nutritive value, reduce mycotoxin contamination, reduce the problems demand solutions such as antibiotic usage.
In recent years, biofermentation is proved to be to improve raw material nutritive value, reduce mycotoxin and ANFs, thus promotes unconventional development of raw materials, reduces antibiotic usage and improve the effective way of animal health.
Current biological fermentation feed mainly concentrates on single fermenting raw materials, in pig complete diet pellet, also have some correlation techniques.As patent of invention (number of patent application: CN201410258693.4) is applicable to the complete feed of many animals by fermenting and producing, but do not take into full account digestive physiological characteristics and the nutrient requirement of different animals, and bacterial classification preparation process is complicated, culture medium cost is high, sweat needs to stir, drying is needed after fermentation, during use will with other complete diet pellets or other raw materials used in combination, program is various, be unfavorable for promote.Related invention patent (the number of patent application: CN201410015788.3 of other fermented feed, license number: CN101940267B) use the multiple nutrients such as enzyme preparation, crystal amino acid and non-nutritive additive, the nutritive value of tunning and quality raw materials can not be made full use of, improve aquaculture cost, and be fermented feed special for piglets, lack general applicability.Technical scheme or the culture medium cost of above-mentioned patent are high, or complete diet pellet quality raw materials cost is high, and all do not relate to the problem reducing mycotoxin contamination.
Sugar industry byproduct molasses contain the sugar more than 45%, also containing abundant vitamin, have good attractant, palatability and nutritive value.In addition, the trophic component of molasses uniqueness makes it can be used as the high-quality substrate of the bacterial classifications such as yeast.But at present molasses limit its a large amount of uses at feedstuff industry due to the characteristic of the place of production and thick liquid.
Summary of the invention
The present invention is directed to growing and fattening and agent of feed for pregnancy sow Middle nutrition and be worth the problem low, mycotoxin easily exceeds standard, antibiotic is excessive, and existing fermented feed technical matters is complicated, cost is high, specificity crosses the deficiencies such as strong, provides and a kind ofly have that general applicability, mycotoxin content are low, simple to operate, the nonreactive pig full price fermented feed of remarkable in economical benefits.
In order to achieve the above object, the invention provides a kind of nonreactive, low mycotoxin, full price fermented feed, be specially:
First in basal feed, multi-strain fermentation agent and molasses are added, then add clear water and carry out mixed diluting, form the mixed fodder of half dry and wet state, finally mixed fodder is loaded polybag, with vavuum pump air extracting seal Anaerobic culturel, period mean temperature be 26-30 DEG C, medial humidity is 75-85%, after fermentation 72h, sampling detects fermented feed pH≤4.4-4.5, and obtains full price fermented feed with during strong sour fragrance.
By mass percentage, described basal feed forms primarily of following component: corn: 60%-70.5%, dregs of beans: 13%-27%, and surplus is wheat bran.
The 1.5%-2.0% of feeding quality based on described multi-strain fermentation agent addition.
The 2%-12% of feeding quality based on described molasses addition.
Described multi-strain fermentation agent by mixing lactic acid bacteria liquid, yeast liquid and bacillus subtilis liquid by volume 2-3:1:1 ratio mix.
Mixed lactobacillus suspension by lactobacillus acidophilus liquid, Lactobacillus brevis liquid, cheese lactobacillus suspension, enterococcus faecalis liquid, lactose lactobacillus liquid by volume 1-2:1-2:1-2:1:1 ratio mix.
Described yeast liquid is obtained by following methods: by saccharomycete 33 DEG C of standing activation culture 9h in YEPD culture medium; Then be inoculated in suitable primary-seed medium by the barms activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h, interval ventilation simultaneously; Again first order seed is proceeded in secondary seed medium and carry out expansion cultivation again, obtain yeast liquid; Described secondary seed medium consists of 10 ° of BX molasses, ammonium sulfate 0.10%, urea 0.10%, potassium dihydrogen phosphate 0.16%, epsom salt 0.05%, and all the other are water.
Described bacillus subtilis liquid is obtained by following methods: by bacillus subtilis 42 DEG C of shaking table activation culture 18h in LB culture medium; Then be inoculated in suitable primary-seed medium by the bacillus subtilis activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h, interval ventilation simultaneously; Again first order seed is proceeded in secondary seed medium and carry out expansion cultivation again, obtain bacillus subtilis liquid; Described secondary seed medium consists of 6 ° of BX molasses, ammonium sulfate 0.2%, urea 0.15%, potassium dihydrogen phosphate 0.2%, NaCl0.4%, and all the other are water.
Described mixing lactic acid bacteria liquid is obtained by following methods: by the 35 DEG C of static activation culture 24-48h in MRS culture medium respectively of the various lactic acid bacterias in mixing lactic acid bacteria liquid; Then be inoculated into respectively in suitable primary-seed medium by the various lactic acid bacterias activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h; Again first order seed is proceeded to respectively in secondary seed medium carry out again expansion cultivate, respectively various lactobacillus suspension, then various lactobacillus suspension is mixed in proportion, namely obtains mixing lactic acid bacteria liquid; Described secondary seed medium consists of 16 ° of BX molasses, urea 0.10%, ammonium sulfate 0.10%, magnesium sulfate 0.04%, potassium dihydrogen phosphate 0.15%, and all the other are water.
The present invention is based on domestic and international progress, with the achievement acquired by pig experiment for foundation, by adding a certain proportion of molasses, for growth of microorganism, utilizing these compound microorganism ferments corn, dregs of beans, wheat brans etc. to make full price fermented feed.Prove through all types of test, fermented feed of the present invention can reduce mycotoxins in feed content (see table 1), improves the nutritive value (see table 2) of feed at a low price, significantly reduces the pig incidence of disease, improve the production performance of pig.Safety of the present invention, simple to operate, effective, remarkable in economical benefits.
Mycotoxin content before and after table 1 feed fermentation
Table 2 feed nutrient table (absolute dry condition)
。
Accompanying drawing explanation
fig. 1biological fermentation feed is on the impact of grower pigs health status.
Detailed description of the invention
Below we in conjunction with detailed description of the invention, the present invention is further illustrated.
Embodiment 1
One boar full price fermented feed is obtained by following preparation method:
(1) configure base daily ration: by mass percentage, basal feed is composed of the following components: corn: 65%, dregs of beans: 20%, wheat bran 15%.
(2) multi-strain fermentation agent is prepared:
1. yeast liquid is prepared: by saccharomycete 33 DEG C of standing activation culture 9h in YEPD culture medium; Then be inoculated in suitable primary-seed medium by the barms activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h, interval ventilation simultaneously; Again first order seed is proceeded in secondary seed medium and carry out expansion cultivation again, obtain yeast liquid; Described secondary seed medium consists of 10 ° of BX molasses, ammonium sulfate 0.10%, urea 0.10%, potassium dihydrogen phosphate 0.16%, epsom salt 0.05%, and all the other are water.
2. bacillus subtilis is prepared: by bacillus subtilis 42 DEG C of shaking table activation culture 18h in LB culture medium; Then be inoculated in suitable primary-seed medium by the bacillus subtilis activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h, interval ventilation simultaneously; Again first order seed is proceeded in secondary seed medium and carry out expansion cultivation again, obtain bacillus subtilis liquid; Described secondary seed medium consists of 6 ° of BX molasses, ammonium sulfate 0.2%, urea 0.15%, potassium dihydrogen phosphate 0.2%, NaCl0.4%, and all the other are water.
3. mixing lactic acid bacteria liquid is prepared: by various lactic acid bacterias (lactobacillus acidophilus liquid, Lactobacillus brevis liquid, cheese lactobacillus suspension, enterococcus faecalis liquid, lactose lactobacillus) the 35 DEG C of static activation culture 24-48h in MRS culture medium respectively in mixing lactic acid bacteria liquid; Then be inoculated into respectively in suitable primary-seed medium by the various lactic acid bacterias activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h; Again first order seed is proceeded to respectively in secondary seed medium and carry out expansion cultivation again, respectively various lactobacillus suspension, then by various lactobacillus suspension in proportion (lactobacillus acidophilus liquid, Lactobacillus brevis liquid, cheese lactobacillus suspension, enterococcus faecalis liquid, lactose lactobacillus liquid by volume 1-2:1-2:1-2:1:1 ratio mixing) mix, namely obtain mixing lactic acid bacteria liquid; Described secondary seed medium consists of 16 ° of BX molasses, urea 0.10%, ammonium sulfate 0.10%, magnesium sulfate 0.04%, potassium dihydrogen phosphate 0.15%, and all the other are water.
By above-mentioned gained mixing lactic acid bacteria liquid, yeast liquid and bacillus subtilis liquid by volume 2:1:1 ratio mix and obtain multi-strain fermentation agent.
(3) ferment:
In basal feed quality, get mass fraction be 2% multi-strain fermentation agent, 2% molasses and basal feed, then add clear water and carry out mixed diluting, form the mixed fodder of half dry and wet state, finally mixed fodder is loaded polybag, with vavuum pump air extracting seal Anaerobic culturel, period, mean temperature was 26-30 DEG C, medial humidity is 75-85%, and after fermentation 72h, sampling detects fermented feed pH≤4.4-4.5, and obtains full price fermented feed with during strong sour fragrance.
Embodiment 2
One boar full price fermented feed is obtained by following preparation method:
(1) configure base daily ration: by mass percentage, basal feed is composed of the following components: corn: 60%, dregs of beans: 13%, wheat bran 27%.
(2) multi-strain fermentation agent is prepared:
1. yeast liquid is prepared: preparation method is with embodiment 1.
2. bacillus subtilis is prepared: preparation method is with embodiment 1.
3. mixing lactic acid bacteria liquid is prepared: preparation method is with embodiment 1.
By above-mentioned gained mixing lactic acid bacteria liquid, yeast liquid and bacillus subtilis liquid by volume 3:1:1 ratio mix and obtain multi-strain fermentation agent.
(3) ferment:
In basal feed quality, get mass fraction be 2% multi-strain fermentation agent, 4% molasses and basal feed, then add clear water and carry out mixed diluting, form the mixed fodder of half dry and wet state, finally mixed fodder is loaded polybag, with vavuum pump air extracting seal Anaerobic culturel, period, mean temperature was 26-30 DEG C, medial humidity is 75-85%, and after fermentation 72h, sampling detects fermented feed pH≤4.4-4.5, and obtains full price fermented feed with during strong sour fragrance.
Embodiment 3
One boar full price fermented feed is obtained by following preparation method:
(1) configure base daily ration: by mass percentage, basal feed is composed of the following components: corn: 70.5%, dregs of beans: 27%, wheat bran 2.5%.
(2) multi-strain fermentation agent is prepared:
1. yeast liquid is prepared: preparation method is with embodiment 1.
2. bacillus subtilis is prepared: preparation method is with embodiment 1.
3. mixing lactic acid bacteria liquid is prepared: preparation method is with embodiment 1.
By above-mentioned gained mixing lactic acid bacteria liquid, yeast liquid and bacillus subtilis liquid by volume 3:1:1 ratio mix and obtain multi-strain fermentation agent.
(3) ferment:
In basal feed quality, get mass fraction be 1.5% multi-strain fermentation agent, 10% molasses and basal feed, then add clear water and carry out mixed diluting, form the mixed fodder of half dry and wet state, finally mixed fodder is loaded polybag, with vavuum pump air extracting seal Anaerobic culturel, period, mean temperature was 26-30 DEG C, medial humidity is 75-85%, and after fermentation 72h, sampling detects fermented feed pH≤4.4-4.5, and obtains full price fermented feed with during strong sour fragrance.
Fermented feed of the present invention is on growing-finishing pig production performance and healthy impact test:
Test selects 3 (every 18 cultivation hurdles, 10, every hurdle pig) average weights to be about the grower pigs of 30kg, is divided into two groups immediately, control group (basal feed) and test group (fermented feed of the embodiment of the present invention 1 gained).Grower pigs free choice feeding and drinking-water, all the other are undertaken by the conventional feeding and management in pig farm.About 40 days experimental periods.
Inspection target: after off-test, calculates the daily gain of pig, daily ingestion amount, feedstuff-meat ratio in units of hurdle.By in units of add up the incidence of disease of pig.
Statistics: after test data Excel2013 carries out edit, adopt SPSS18.0 software to carry out t inspection to test data, data are expressed as average ± standard error, are considered as biometric significant difference with P < 0.05.
Result of the test: production performance the results are shown in Table 3, test group grower pigs daily gain, daily ingestion amount are significantly higher than control group as can be seen from Table 3, and feedstuff-meat ratio is significantly lower than control group.,
Table 3 biological fermentation feed is on the impact of grower pigs production performance
Note: the female different expression significant difference of shoulder marking-up, lower same.
Health status the results are shown in Figure 1, and test group grower pigs in condition as can be seen from Figure 1, and the incidence of disease is lower than control group.
Embodiment 2,3 has also been carried out to the impact test of corresponding production performance and health status, result of the test and embodiment 1 result without significant difference, therefore do not do deposited stating at this.
Claims (10)
1. a boar full price fermented feed, is characterized in that: this full price fermented feed is obtained by following preparation method:
First in basal feed, multi-strain fermentation agent and molasses are added, then add clear water and carry out mixed diluting, form the mixed fodder of half dry and wet state, finally mixed fodder is loaded polybag, with vavuum pump air extracting seal Anaerobic culturel, after fermentation 72h, sampling detects fermented feed pH≤4.4-4.5, and obtains full price fermented feed with during strong sour fragrance.
2. a boar full price fermented feed according to claim 1, it is characterized in that: by mass percentage, described basal feed forms primarily of following component: corn: 60%-70.5%, dregs of beans: 13%-27%, and surplus is wheat bran.
3. a boar full price fermented feed according to claim 1, is characterized in that the 1.5%-2.0% of feeding quality based on multi-strain fermentation agent addition.
4. a boar full price fermented feed according to claim 1, is characterized in that the 2%-12% of feeding quality based on molasses addition.
5. a boar full price fermented feed according to claim 1, during it is characterized in that described Anaerobic culturel, temperature is 26-30 DEG C, and humidity is 75-85%.
6. the boar full price fermented feed according to claim 1 or 3, it is characterized in that multi-strain fermentation agent by mixing lactic acid bacteria liquid, yeast liquid and bacillus subtilis liquid by volume 2-3:1:1 ratio mix.
7. a boar full price fermented feed according to claim 6, it is characterized in that mixing lactic acid bacteria liquid by lactobacillus acidophilus liquid, Lactobacillus brevis liquid, cheese lactobacillus suspension, enterococcus faecalis liquid, lactose lactobacillus liquid by volume 1-2:1-2:1-2:1:1 ratio mix.
8. a boar full price fermented feed according to claim 6, is characterized in that:
Described yeast liquid is obtained by following methods:
By saccharomycete 33 DEG C of standing activation culture 9h in YEPD culture medium; Then be inoculated in culture medium by the barms activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h, interval ventilation simultaneously; Again first order seed is proceeded in secondary seed medium and carry out expansion cultivation again, obtain yeast liquid; Described secondary seed medium consists of 10 ° of BX molasses, ammonium sulfate 0.10%, urea 0.10%, potassium dihydrogen phosphate 0.16%, epsom salt 0.05%, and all the other are water.
9. a boar full price fermented feed according to claim 6, is characterized in that:
Described bacillus subtilis liquid is obtained by following methods:
By bacillus subtilis 42 DEG C of shaking table activation culture 18h in LB culture medium; Then be inoculated in culture medium by the bacillus subtilis activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h, interval ventilation simultaneously; Again first order seed is proceeded in secondary seed medium and carry out expansion cultivation again, obtain bacillus subtilis liquid; Described secondary seed medium consists of 6 ° of BX molasses, ammonium sulfate 0.2%, urea 0.15%, potassium dihydrogen phosphate 0.2%, NaCl0.4%, and all the other are water.
10. a boar full price fermented feed according to claim 7, is characterized in that:
Described mixing lactic acid bacteria liquid is obtained by following methods:
By the 35 DEG C of static activation culture 24-48h in MRS culture medium respectively of the various lactic acid bacterias in mixing lactic acid bacteria liquid; Then be inoculated in culture medium respectively by the various lactic acid bacterias activated and prepare first order seed, inoculum concentration is 8-10%, temperature 35-42 DEG C, cultivates 48-60h; Again first order seed is proceeded to respectively in secondary seed medium carry out again expansion cultivate, respectively various lactobacillus suspension, then various lactobacillus suspension is mixed in proportion, namely obtains mixing lactic acid bacteria liquid; Described secondary seed medium consists of 16 ° of BX molasses, urea 0.10%, ammonium sulfate 0.10%, magnesium sulfate 0.04%, potassium dihydrogen phosphate 0.15%, and all the other are water.
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CN105767530A (en) * | 2016-03-25 | 2016-07-20 | 武汉明天生物科技有限公司 | Solid state fermentation feed technology and related high-efficiency safe biological fermentation feed |
CN106387309A (en) * | 2016-08-31 | 2017-02-15 | 西安若翰生物饲料有限公司 | High-efficient bio-fermented feed |
CN106509338A (en) * | 2016-11-02 | 2017-03-22 | 南京宝辉生物饲料有限公司 | Biological fermentation feed |
CN106721278A (en) * | 2016-12-07 | 2017-05-31 | 天津牧丰饲料有限公司 | Microbial fermentation child care phase piglet liquid-state feed and preparation method and application |
CN106889330A (en) * | 2017-03-01 | 2017-06-27 | 海南青牧原实业有限公司 | A kind of production method of nonreactive fermented feed for fattening pigs |
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CN107455569A (en) * | 2017-09-18 | 2017-12-12 | 陈雨 | A kind of fermentative feedstuff of microbe and preparation method thereof |
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CN107691839A (en) * | 2017-09-25 | 2018-02-16 | 江西省科学院微生物研究所 | A kind of green non-pollution fattening pig premix and preparation method and application |
CN107751608A (en) * | 2017-10-20 | 2018-03-06 | 武汉市天发有机肥有限公司 | One boar food |
CN108835427A (en) * | 2018-08-15 | 2018-11-20 | 西北农林科技大学 | A kind of pregnant sow full price fermentative feedstuff of microbe preparation method |
CN109699813A (en) * | 2019-03-04 | 2019-05-03 | 广东省农业科学院动物科学研究所 | It is a kind of to improve the solid state fermentation complete diet pellet for fattening meat quality and its preparation method and application |
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