CN107691839A - A kind of green non-pollution fattening pig premix and preparation method and application - Google Patents
A kind of green non-pollution fattening pig premix and preparation method and application Download PDFInfo
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- CN107691839A CN107691839A CN201710876399.3A CN201710876399A CN107691839A CN 107691839 A CN107691839 A CN 107691839A CN 201710876399 A CN201710876399 A CN 201710876399A CN 107691839 A CN107691839 A CN 107691839A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
Abstract
The invention discloses a kind of green non-pollution to fatten pig premix, is made up of the raw material of following proportionings：Lactobacterium acidophilus are more than 0.5 trillion cfu；Enterococcus faecalis is more than 0.3 trillion cfu；Clostridium butyricum is more than 0.15 trillion cfu；4.8~6.2kg of salt；6.2~10.5kg of calcium monohydrogen phosphate；8.8~12.6kg of calcium carbonate；245~355g of biological polyoses；The IU of vitamin A 17.0~19.0 ten thousand；The IU of vitamine D3 2.8~3.2 ten thousand；420~460mg of nicotinic acid；220~228mg of calcium pantothenate；20kg is supplemented to powdered rice hulls, is the green non-pollution fattening pig premix of 2% addition after being well mixed.The present invention improves the gut flora balance of pig, strengthens pig autoimmunity ability, improves the pig general level of the health and the speed of growth, improves efficiency of feed utilization and reduces feedstuff-meat ratio.
Description
Technical field
The invention belongs to cultural technique field, is related to a kind of green non-pollution fattening pig premix and preparation method and answers
With.
Background technology
Preventative and growth promotion medicine addition has greatly facilitated the development of aquaculture industry of China in feed and aquaculture, supports
It is maximized simultaneously in seeking results to grow industry owner, also brings serious problem of environmental pollution.The country more than 95% at present
Premix product adds growth promotion and feeding antibiotic.In about 210,000 tons of antibiotic raw materials that China produces every year according to statistics
There are 9.7 ten thousand tons to be used for livestock breeding industry, feeding antibiotic creates huge economic benefit to aquaculture, to China or even the world
The development of animal husbandry is made that huge contribution.But antibiotic is added in premix and blindly abuse is anti-in breeding process
Raw element, causes various pathogenic microorganisms to develop immunity to drugs, and the suprainfection of animal, has a strong impact on livestock birds health situation；Also
Animal products drug residue can be caused, not only influence the quality and its security of animal products, also resulted in sick in mankind's body
Pathogenic microorganism produces drug resistance to antibiotic, and then directly threatens human health.European Union and the U.S. have been completely forbidden in feed
Middle addition antibiotic, The Ministry of Agriculture of the People's Republic of China, MOA formally issue No. 2292 bulletin：Forbid Lomefloxacin, Pefloxacin,
4 kinds of Ofloxacin, Norfloxacin antibiotic use in food animal, State Administration of Quality Supervision, Inspection and Quarantine's issue No. 144 bulletin in 2015, to entering
Outlet feed and feed addictive risk class and inspection and quarantine supervision method are made adjustment, and country puts into effect multinomial phase in succession
Pass standard and industrial policy, it is coming that Chinese feed prohibits the anti-epoch.
Many pig-breeding enterprises are during scale animal and poultry cultivation, to accelerate growth of animals or poultry speed, improving feed profit
With rate and livestock and poultry is prevented, largely using trace elements such as Cu, Zn, Fe in feed addictive.But because pig is to metal member
The low digestibility of element, a large amount of metallic elements will excrete in the form of excrement, and in excrement heavy metal a large amount of discharges
It will cause seriously to pollute to environment.With the continuous enhancing of the nuisanceless consciousness of people and idea, through foring to safety
Health, noresidue, the active demand of pollution-free, healthy pollution-free food.
Additive for microbe feedstuff is the feed addictive of Ministry of Agriculture's approval, can not only tackle the complicated life of cultivated animals
The multiple challenges such as rationality diarrhoea, trophism diarrhea and pathogenicity diarrhoea, can also control Animal diseases, promote growth of animal, increase
Strong immunity function, the multi-efficiency, especially young animal table such as improve Nutrition and Metabolism, improve the price of deed, reduce environmental pollution
Reveal good advantage, play the growth performance of domestic animal, improve human body immune function, improve gut flora balance, control diarrhea rate
And the incidence of disease.Additive for microbe feedstuff is that effectively solve, and disease is spread unchecked, germ resistance, immunocompetence decline, survival rate drop
The effective means that low, culture benefit declines, it is the power of sustainable development of animal husbandry.But such a product is supported in animal mostly
Single culture or simple combination use as additive agent for feeding during growing, not according to different animals in different physiological stages
Growth characteristics to different strain and the needs of promotive factor, and added in feed formula, it is thus impossible to effectively reach reduction
Or substitute the purpose that feeding antibiotic and trace meter are added in feed.
The content of the invention
The technical problems to be solved by the invention are to be directed to above-mentioned problem, there is provided one kind not only possessed nutritive effect but also
There is the green non-pollution fattening pig premix of animal immune healthcare function, compared with existing product, by adding probiotics and benefit
Raw member, improve the gut flora balance of pig, strengthen pig autoimmunity ability and greatly reduce diarrhea rate and the incidence of disease, so as to carry
High the pig general level of the health and the speed of growth, improve efficiency of feed utilization and reduce feedstuff-meat ratio；Effectively substitute feeding antibiotic medicine and
Addition of the trace element in feed, solve the harm of germ resistance and excrement heavy metal to environment, promote human health, improve
Ecological environment.
It is a further object of the present invention to provide the preparation method of green non-pollution fattening pig premix.
Third object of the present invention is in the application in offer green non-pollution fattening pig premix in growing-finishing pigs.
The present invention's is achieved through the following technical solutions.
A kind of green non-pollution fattens pig premix, is made up of the raw material of following proportionings：
Lactobacterium acidophilus are more than 0.5 trillion cfu；
Enterococcus faecalis is more than 0.3 trillion cfu；
Clostridium butyricum is more than 0.15 trillion cfu；
4.8~6.2kg of salt；
6.2~10.5kg of calcium monohydrogen phosphate；
8.8~12.6kg of calcium carbonate；
245~355g of biological polyoses；
The IU of vitamin A 17.0~19.0 ten thousand；
The IU of vitamine D3 2.8~3.2 ten thousand；
420~460mg of nicotinic acid；
220~228mg of calcium pantothenate；
20kg is supplemented to powdered rice hulls, is the green non-pollution fattening pig premix of 2% addition after being well mixed.
Preferably, it is made up of the raw material of following proportionings：
Lactobacterium acidophilus are more than 0.5 trillion cfu；
Enterococcus faecalis is more than 0.6 trillion cfu；
Clostridium butyricum is more than 0.15 trillion cfu；
Salt 6.2kg；
Calcium monohydrogen phosphate 10.5kg；
Calcium carbonate 12.6kg；
Biological polyoses 245g；
The IU of vitamin A 18.0 ten thousand；
The IU of vitamine D3 3.0 ten thousand；
Nicotinic acid 440mg；
Calcium pantothenate 224mg；
20kg is supplemented to powdered rice hulls, is the green non-pollution fattening pig premix of 2% addition after being well mixed.
Preferably, it is made up of the raw material of following proportionings：
Lactobacterium acidophilus are more than 1.2 trillion cfu；
Enterococcus faecalis is more than 0.3 trillion cfu；
Clostridium butyricum is more than 0.25 trillion cfu；
Salt 4.8kg；
Calcium monohydrogen phosphate 6.2kg；
Calcium carbonate 8.8kg；
Biological polyoses 335g；
The IU of vitamin A 18.0 ten thousand；
The IU of vitamine D3 3.0 ten thousand；
Nicotinic acid 440mg；
Calcium pantothenate 224mg；
20kg is supplemented to powdered rice hulls, is the green non-pollution fattening pig premix of 2% addition after being well mixed.
A kind of preparation method of above-mentioned green non-pollution fattening pig premix, it is characterised in that comprise the following steps：
(1), according to proportioning, raw material calcium monohydrogen phosphate, calcium carbonate, salt are weighed, calcium monohydrogen phosphate, calcium carbonate, salt are mixed
Mixture carrier one is uniformly mixing to obtain, it is standby；
(2) raw material biological polyoses, are weighed, carry out required vitamin A, vitamine D3, nicotinic acid, calcium pantothenate further according to proportioning
It is uniformly mixed, is put into together with the 0.1-50% of the mixture carrier one then obtained with step (1) in mixing plant container
It is stirred, mixes 5~25 minutes, obtain mixture carrier two；
(3), lactobacterium acidophilus, enterococcus faecalis, the living bacteria count of clostridium butyricum according to needed for proportioning, weigh corresponding bacterium
Powder, it is put into mixing plant container together with the 0.1-50% of the mixture carrier one obtained with step (1) and is stirred, stirring is mixed
Close 15~50 minutes, obtain mixture carrier three.
(4), mixture carrier three, the mixture that mixture carrier two that step (2) obtain, step (3) will be obtained again
Surplus of the carrier one by step (2) and step (3) is put into mixing plant container together, is stirred 5~45 minutes, that is, is made
Obtain green non-pollution fattening pig premix.
Preferably, comprise the following steps：
(1), according to proportioning, raw material calcium monohydrogen phosphate, calcium carbonate, salt are weighed, calcium monohydrogen phosphate, calcium carbonate, salt are mixed
Mixture carrier one is uniformly mixing to obtain, it is standby；
(2) raw material biological polyoses, are weighed, carry out required vitamin A, vitamine D3, nicotinic acid, calcium pantothenate further according to proportioning
Be uniformly mixed, the mixture carrier one then obtained with step (1) 50% together be put into mixing plant container and stir
Mixing, mix 5~25 minutes, obtain mixture carrier two；
(3), lactobacterium acidophilus, enterococcus faecalis, the living bacteria count of clostridium butyricum according to needed for proportioning, weigh corresponding bacterium
Powder, the mixture carrier one obtained with step (1) other 50% together be put into mixing plant container and be stirred, stirring is mixed
Close 15~50 minutes, obtain mixture carrier three.
(4), the mixture carrier three that mixture carrier two that step (2) obtain, step (3) obtain will be put into together again
In mixing plant container, it is stirred 5~45 minutes, that is, green non-pollution fattening pig premix is made.
A kind of application of above-mentioned green non-pollution fattening pig premix in growing-finishing pigs.
Compared with prior art, it is an advantage of the invention that：
1) present invention is added by lactobacterium acidophilus, enterococcus faecalis, clostridium butyricum in green non-pollution fattens pig premix
The compound micro-ecological preparation of composition realizes the antibiosis substituted completely in feed and breeding process for the purpose of disease-resistant growth-promoting
Plain medicine and micro- (such as Cu, Mn, Fe) addition use, and effectively solve the danger of germ resistance and excrement heavy metal to environment
Evil, promote human health, improve the ecological environment.
2) green non-pollution fattening pig premix of the present invention, having improves pig gut flora balance, strengthens pig itself
Immunocompetence and greatly reduction diarrhea rate and the incidence of disease, so as to improve the pig general level of the health and the speed of growth, improve feed conversion
Rate and reduction feedstuff-meat ratio；
3) use of antibiotic medicine and micro- (such as Cu, Mn, Fe) is added in feed due to substituting, is produced
Not only content of beary metal is low and flavor quality is good for pork, and Health security system is nuisanceless, meet country's promotion exploitation and
Promote the developing direction of " nonreactive feed ".
Brief description of the drawings
Fig. 1 is that green non-pollution of the present invention fattens influence of the pig premix to growing-finishing pigs performance；
Fig. 2 is that green non-pollution of the present invention fattens influence of the pig premix to growing-finishing pig blood parameters；
Fig. 3 is that green non-pollution of the present invention fattens influence of the pig premix to pH value in growing and fattening swine alimentary canal；
Fig. 4 is that green non-pollution of the present invention fattens content of beary metal ratio of the pig premix to growing and fattening in swinery excrement
Compared with analysis；
Fig. 5 is that green non-pollution of the present invention is fattened pig premix and heavy-metal residual of the growing and fattening in lean meat compared point
Analysis；
Fig. 6 is that green non-pollution of the present invention fattening pig premix compares heavy-metal residual of the growing and fattening in pig liver
Analysis；
Fig. 7 is bifidobacterium adolescentis liquid fermentation process flow of the present invention；
Fig. 8 is bifidobacterium adolescentis leaven line chart of the present invention.
Embodiment
The present invention is expanded on further below in conjunction with specific embodiment.Involved test method or analysis in following embodiments
Method, it is conventional method unless otherwise instructed, agents useful for same is commercially available unless otherwise instructed, but not as the present invention's
Limit.
First, biological polyoses are prepared
1st, bifidobacterium adolescentis liquid fermentation, as shown in figure 1,
(1) shake-flask seed culture
Bifidobacterium adolescentis (the Chinese industrial Microbiological Culture Collection management of a little inclined-plane seed activation is dipped with oese
Center, CICC 6175) access seed culture medium (glucose 10.0g/L, starch 3.0g/L, peptone 12.0g/L, yeast extraction
Thing 8.0g/L, beef extract 5g/L, K2HPO41.0g/L, sodium acetate 4.0g/L, MgSO4·7H2O 0.2g/L, FeSO4·7H2O
0.15g/L, MnSO4·H2O 0.05g/L) in, in 37 DEG C, cultivate 12h, as seed liquor；
(2) seed tank culture
Seed tank volume is 30L, fills liquid seed culture medium 25L, 115 DEG C of sterilising temp, 20min；Shake-flask seed is trained
Support obtained seed liquor and enter seeding tank according to 2% inoculation volume ratio (i.e. 500ml), fermentation parameter is：37 DEG C of temperature, rotating speed
100r/min, initial pH 7.0, pressure 0.05Mpa is closed, uses 40%Na2CO3Constant pH is not less than 6.5；Cultivate 13h, as two level kind
Sub- liquid.The formula of the seed tank culture base is：Glucose 10.0g/L, starch 3.0g/L, peptone 12.0g/L, yeast extraction
Thing 8.0g/L, beef extract 5g/L, K2HPO41.0g/L, sodium acetate 4.0g/L, MgSO4·7H2O 0.2g/L, FeSO4·7H2O
0.15g/L, MnSO4·H2O 0.05g/L。
(3) fermentation tank culture
Fermenter volume is 1 500L, loads the 200L of fermentation medium 1,115 DEG C of sterilising temp, 20min；By seeding tank
Cultivate obtained seed culture fluid and be all pumped into fermentation tank, fermentation parameter is：37 DEG C of temperature, rotating speed 150r/min, initial pH
7.5；Start to close pressure 0.05MPa, treat that pH is down to 6.8, be changed into negative pressure 0.06MPa and continue to cultivate；Whole fermentation period Na2CO3It is permanent
PH is determined not less than 6.7；The formula of the seed tank culture base is：Sucrose 40.0g/L, peptone 15.0g/L, yeast extract
10.0g/L, beef extract 8.0g/L, K2HPO42.0g/L, sodium acetate 4.0g/L, MgSO4·7H2O 0.35g/L, FeSO4·
7H2O 0.25g/L, MnSO4H2O 0.15g/.
(4) Fermentative growth curve
Above-mentioned culture is sampled every 1h, with TPY agar medium plate count cultures, and draws its Fermentative growth song
Line, as shown in figure 8, the cell concentration highest when culture is to 18h, reaches 3.45 × 1010cfu/ml
(5) separation and Extraction of biological polyoses
20ml zymotic fluids are taken, 15min is centrifuged under 12 000rpm；Collection supernatant, 2 times of volume absolute ethyl alcohols of addition, 4
DEG C 12h is stood, centrifuge 20min under 12 000rpm, obtain polysaccharide precipitation, with organic solvent washings such as ethanol 3 times, at 70 DEG C
Under be dried to constant weight.When culture is to 19h, biological polyoses reach 0.031g/mL
2nd, prepared by biological polyoses
By bifidobacterium adolescentis zymotic fluid obtained above through GQ105R high speed tube centrifuges, 13000r/min is by thalline
Cell precipitation, collect fermentation clear liquid；
5% corn flour is added in above-mentioned collection zymotic fluid clear liquid as filler, with the 20L/h speed stream added-time, with reference to
Operating efficiency, selecting optimal EAT, feed temperature is 50 DEG C, hot blast rate 20m for 170 DEG C3/ h, rotating speed of shower nozzle are
25000r/min, temperature is 45 DEG C in collector.Fermentation clear liquid is spray-dried, it is biological polyoses to obtain powdered, production
Measure as 81.25g/L.
2nd, premix is prepared
A kind of green non-pollution fattens pig premix, is made up of the raw material of following proportionings：
Lactobacterium acidophilus are more than 1.2 trillion cfu；
Enterococcus faecalis is more than 0.3 trillion cfu；
Clostridium butyricum is more than 0.25 trillion cfu；
Salt 4.8kg；
Calcium monohydrogen phosphate 6.2kg；
Calcium carbonate 8.8kg；
Biological polyoses 335g；
The IU of vitamin A 18.0 ten thousand；
The IU of vitamine D3 3.0 ten thousand；
Nicotinic acid 440mg；
Calcium pantothenate 224mg；
20kg is supplemented to powdered rice hulls, is the green non-pollution fattening pig premix of 2% addition after being well mixed.
Green non-pollution fattening pig premix is prepared according to said ratio, comprised the following steps：
(1), according to proportioning, raw material calcium monohydrogen phosphate, calcium carbonate, salt are weighed, calcium monohydrogen phosphate, calcium carbonate, salt are mixed
Mixture carrier one is uniformly mixing to obtain, it is standby；
(2) raw material biological polyoses, are weighed, carry out required vitamin A, vitamine D3, nicotinic acid, calcium pantothenate further according to proportioning
Be uniformly mixed, the mixture carrier one then obtained with step (1) 0.1% together be put into mixing plant container and stir
Mixing, mix 15 minutes, obtain mixture carrier two；
(3), lactobacterium acidophilus, enterococcus faecalis, the living bacteria count of clostridium butyricum according to needed for proportioning, weigh corresponding bacterium
Powder, the mixture carrier one obtained with step (1) 1% together be put into mixing plant container and be stirred, be stirred 30
Minute, obtain mixture carrier three.
(4), mixture carrier three, the mixture that mixture carrier two that step (2) obtain, step (3) will be obtained again
Surplus (i.e. 100%-0.1%-1%=98.9%) of the carrier one by step (2) and step (3) is put into mixing plant appearance together
In device, it is stirred 30 minutes, that is, green non-pollution fattening pig premix is made.
Basal diet (such as corn and dregs of beans) is pre-processed, through cleaning, after crushing, weight is required according to mix designs
Proportioning, mixing is stirred, after mixing, that is, innoxious green growing and fattening pigs complete diet pellet is made.
Wherein, can be toward obtained mixture carrier one be added in step (1) in the mixture carrier two in step (2) again
1%~2% be stirred mixing, mix 5~25 minutes；Obtained mixture is added in step (1) after the completion of mixing again
The 10%~20% of carrier one is stirred mixing, mixes 5~25 minutes；Add in step (4) and mix after the completion of mixing.
Wherein, can be toward obtained mixture carrier one be added in step (1) in the mixture carrier three in step (3) again
10%~22% be stirred mixing, mix 15~50 minutes, add in step (4) and mix after the completion of mixing.
Wherein, the mixture carrier one of step (1) can be divided into two parts, a part is used for and the dimension in step (2)
Raw plain A, vitamine D3, nicotinic acid, calcium pantothenate, which are stirred to be well mixed, obtains mixture carrier two；Another part is used and in step
(3) lactobacterium acidophilus, enterococcus faecalis, the corresponding bacterium powder of the living bacteria count of clostridium butyricum according to needed for proportioning in are stirred
It is well mixed to obtain mixture carrier three；Carried in the mixture that the mixture carrier two and step (3) that obtain step (2) obtain
Body three mixes.
Experiment
This experiment from original body mass be (50.21 ± 0.97) kg healthy disease-free, upgrowth situation well (Du × length ×
Tri-crossbreeding 300 greatly), by condition of similarity such as body weight, sexes, 3 groups are randomly divided into, each organize 4 repetitions, each repeatedly 25
Head pig.August starts to test on the 13rd within 2015, lasts 75 days,
1st, daily ration and design are tested
Experiment sets 3 treatment groups, control group I (conventional material) altogether：Basal diet is prepared with reference to NRC (2010)；Control group II
(heavy metal containing anti-group-containing the high zinc of high-copper and antibiotic aureomycin)：Ensureing NRC (2010) preparation basal diet nutritional requirements
Under, Cu, Mn, Zn, Fe addition are improved, while also added antibiotic (aureomycin) into basal diet；Test group (this
The green non-pollution fattening pig premix of invention)：It is green using 2% addition of the present invention that basal diet is prepared with reference to NRC (2010)
Color non-polluted growing and fattening pig premix, the specific formula for testing daily ration are shown in Table 1
Table 1 tests the formula and trophic level of daily ration
3rd, feeding management
Enter 7d before column, clean and sterilize column home.Preliminary trial period 7d, it is positive to try phase 75d.Group feeding, daily feeding 4 times, feed metering
It is quantity-unlimiting, using crib without remaining material as principle.Ensure sufficient cleaning drinking-water, pig house is swept daily 2 times, observe adopting for pig in time
Situations such as food and diarrhoea.Carried out according to conventinal breeding and immune programme for children.
4th, data statistics and analysis
Single factor test variance statistic analysis is carried out with SPSS17.0 softwares, minimum significance test is carried out with Duncan's methods,
α=0.05 is taken, is as a result represented with average ± standard deviation, P ＜ 0.05 are significant difference, and P ＜ 0.01 are that pole conspicuousness is poor
It is different.
Interpretation of result
1st, growth performance index
1. feed intake：Each column pig feed consumption rate is recorded daily, calculates the average feed intake of each column pig.
2. daily gain：Each column pig quality at the beginning and end of the positive examination phase is recorded, calculates each column pig average weight gain
As shown in figure 1, test group (growing and fattening pigs green ecological material of the present invention) and control group I (conventional to expect) and control group II
(material containing the high zinc of high-copper with antibiotic) is compared, and 24.4% and 4.1% has been respectively increased in the average daily gain of growing-finishing pig, material
Meat ratio reduces 22.2% and 15.7% (P respectively<0.01).
After the green non-pollution fattening pig premix feeding growing-finishing pig of the present invention, growing and fattening is remarkably improved
Pig average daily gain and reduction average daily gain (P<0.05) its feedstuff-meat ratio (P, is significantly reduced<0.01), further demonstrate
Probiotics is inhaled as dominant microflora in chitling road by suppressing growth of pathogenic bacteria, promoting intestinal microbial balance, improvement to digest
Function is received, so as to improve growth performance；On the other hand it is the life for existing for lactic acid bacteria of the aerobic bacterias such as saccharomycete and bacillus
Long breeding creates anaerobic environment, and lactic acid bacteria amount reproduction generates lactic acid, and provides many bioactive substances, such as function
Macromolecular substances in the thick protein for being difficult to digest and assimilate in feed, starch can be resolved into suction easy to digest by property polypeptide, enzyme etc.
The small molecule nutriment such as glucose, amino acid and vitamin of receipts, so as to improve the digestibility of feed and nutritive value,
Improve the growth performance of pig.In addition, the action effect of fattening pannage of the present invention is significantly better than containing the high zinc of high-copper and antibiotic
Feed, illustrate using the present invention green non-pollution fattening pig premix can save trace element (such as zinc, manganese, copper, iron,
Deng heavy metal element) addition, the use to growth promotion medicine aureomycin can also be reduced.
2nd, blood parameters
Early morning extracts 6 pig vena cava anteriors each 5ml of blood sampling out at random from each group on an empty stomach during off-test, and blood sample is in culture dish
37 DEG C of water-baths are stood, and are waited after separating out serum, draw serum in centrifuge tube, and 3 000r/min centrifugations 15min obtains blood serum sample.Will
Each blood serum sample is sub-packed in pipe and immersed in liquid nitrogen, is placed in -30 DEG C of refrigerators and saves backup after snap frozen processing, freezen protective
Serum.Following index is determined using the automatic clinical chemistry analyzer of Hitachi 7020：Alkaline phosphatase, using enzyme serial testing；
Alanine aminotransferase, using enzyme serial testing；Total protein, using biuret method；Albumin, using Bromocresol green；Total courage
Sterol, colorimetric method is coupled using enzyme；Urea nitrogen, using enzyme coupling rate method.
The change of pig blood biochemical indicator is that histocyte permeability changes and changed with body metabolism function
The reflection of change.The activity of alanine aminotransferase and alkaline phosphatase can reflect metabolism of the livestock and poultry to protein and lipid in serum
Efficiency, particularly alkaline phosphatase, as the isoenzymes with genetic marker, its active height can reflect the growth speed of animal
Degree and production performance.
As shown in Fig. 2 test group (growing and fattening pigs green ecological material of the present invention) and control group I (conventional to expect) and control group II
(material containing the high zinc of high-copper and antibiotic), 30.52% and 21.29% (P has been respectively increased in Serum alkaline phosphatase activity<
0.01), 23.03% and 15.94%, significant difference (P has been respectively increased in alanine transaminase activity<0.01).Raised in this experiment
The alkaline phosphatase and alanine transaminase activity of feed additives group are significantly higher than other each group (P<0.01), also further confirm
The feed addictive of the present invention can improve the growth performance of pig.
Total protein concentration of serum and blood serum urea nitrogen concentration can accurately reflect the metabolism of animal vivo protein and ammonia
Equilibrium condition between base acid, total protein concentration of serum rises when amino acid balance is good and blood serum urea nitrogen concentration declines.
Total protein concentration of serum is 59.6 ± 2.01g/L in test group (growing and fattening pigs green ecological material of the present invention), compared to pair
According to I (conventional material group) of group and control group II (material containing the high zinc of high-copper and antibiotic), total protein concentration of serum is respectively increased
20.16% and 23.91% (P<0.01)；Blood serum urea nitrogen concentration is 2.99 in test group (growing and fattening pigs green ecological material of the present invention)
± 0.15mmol/L, compared to control group I (conventional material group) and control group II (material containing the high zinc of high-copper and antibiotic), serum urea
Nitrogen content reduces 19.58% and 15.04% (P respectively<0.01)；Albumin and globulin are high apparently higher than control group and high-copper
Zinc contains anti-group of (P<0.05)；T-CHOL is less than control group, and difference is obvious, can reduce people and eat after such pork to cholesterol
Intake under, effectively prevent some diseases because caused by edible pork causes cholesterol intake excessive.This result of the test table
The total protein concentration of serum of bright feed additives group is significantly higher than other each group (P<0.01) it is substantially less than with blood serum urea nitrogen concentration
Other each group (P<0.01), show that feed addictive of the present invention can improve the protein synthesis of growing and fattening pigs, increase body nitrogen sinks
Accumulated amount, promote growth.
3rd, pH value changes in growing and fattening swine alimentary canal
Alimentary canal content pH is (smart using the PHS-3C types precision acidity meter of Shanghai precision instrumentation Co., Ltd production
Exactness ± 0.01) it is measured.
As shown in figure 3, since stomach, to gastral end (rectum), have selected Stomach duodenum, small intestine, caecum,
The content at five positions of rectum is measured, and is found：The pH value of test group (growing and fattening pigs green ecological material of the present invention) all compares
It is low according to group I (conventional material), decline 0.12,0.17,0.36,0.43,0.59 respectively.Further illustrate and feed growing and fattening pigs of the present invention
Green ecological premix generates organic acid in swine alimentary canal, Enteron Acidity is had significant reduction, more to alimentary canal
End, pH value reduce more obvious.
4th, content of beary metal situation of the growing-finishing pig in swinery excrement compares
Weigh dried sample 0.5g to boil in pipe in disappearing, add 5mL concentrated nitric acid soaked overnights.Next day, disappeared with temperature control and boiled
Stove, which disappears, boils sample, and for temperature control within 120 DEG C, the liquid boiled into pipe that disappears is limpid, and the high chlorine of 2mL is added after being cooled to room temperature
Acid, continue to disappear and boil and by temperature control within 220 DEG C, cooled down when liquid in pipe is limpid, 50mL is settled to deionized water,
Liquid sample after constant volume is mixed, filtering (0.45 μm of aperture glass filter membrane), analyzed using ICP-MS.Each sample does 3
Duplicate Samples.
Cu is a kind of antiseptic and bone tonic, and in pig body key enzyme needed for a variety of metabolism confactor, directly
Connect and participate in the metabolic processes such as cholesterol metabolic, mineralization of skeleton, immunity function regulation.Zn is the component and activator of a variety of enzymes, no
Only may participate in the multiclass metabolic process of livestock and poultry, the Zn of high dose can also reduce diarrhea rate, alleviate broiler chick caused by diarrhoea and
The change of Intestinal permeabiligy, maintains the normal function of Gut barrie r, in swine rationses the addition high zinc of high-copper can promote pig fast-growth and
Prevent Animal diseases.However, because bioavailability is limited, most of heavy metal element can excrete with animal wastes.
As shown in figure 4, test group (growing and fattening pigs green ecological material of the present invention) is not added with inorganic metal in basal diet, examination
Existing test group copper, manganese, content of zinc in excrement is issued after examination and approval to be substantially less than with control group I (conventional material group) and control group II (containing height
The material of the high zinc of copper and antibiotic), and be far below《Feces of livestock and poultry uses criterion safely》Heavy metal in (NY/T 1334-2007)
The requirement of content.
For test group compared with control group I and control group II, the heavy metal Cu in swinery excrement reduces by 2.2 times and 25 respectively
Times, the heavy metal Mn in swinery excrement reduces by 1.5 times and 23 times respectively, and the heavy metal Zn in swinery excrement reduces 32.1% respectively
Times and 6.6 times, the P in swinery excrement reduces 49.04% and 37.5% (P respectively<0.05).
The heavy metal additive of pig feed middle and high concentration, the heavy-metal residual of high content in pig manure is caused, gives its resource
Change using very big limitation and risk is brought, further checking can be improved to organic source using the fattening pannage of the present invention
Micro effective utilization, while substitute addition of the inorganic heavy metal in basal diet.
5th, heavy-metal residual analysis of the growing-finishing pig in lean meat
Meat is cleaned up, the debris such as surface bloodstain, hair is removed, minces, then weighs 2.0g meat products respectively in white
In color sample dissolving cup, add 5mL concentrated nitric acids, 1mL hydrogen peroxide, enlarging, tinning, counteracting tank is placed in microwave dissolver, setting is micro-
Ripple resolution program is cleared up, and takes out sample dissolving cup afterwards, will clarify bright solution and is settled in 25mL volumetric flasks and is measured,
And blank control is done simultaneously.Lead, cadmium, Cupper determination use micro-wave digestion-graphite furnace flame atomic absorption spectrometry；Arsenic determines：
Standard arsenic spot method；Sclera remodeling：Dithizone colorimetric method.The measure of different heavy metals is national standard detection method.
《National food safety standard pollutants in food is limited the quantity》(GB2762-2012) to cadmium (meat in poultry meat and internal organ
0.1mg/kg；Liver 0.5mg/kg), lead (meat 0.2mg/kg；Internal organ 0.5mg/kg), mercury (0.05mg/kg), arsenic (0.5mg/kg) and
《Copper tolerance limit in food》Copper (10mg/kg) is provided with limit standard in (GB 15199-1994) meat, with《It is fresh
(jelly) poultry meat sanitary standard》(GB2707-2005) to poultry meat and《Pollution-free food pork》(NY 5029-2008) is to pig
Meat lead, cadmium are consistent with the limit standard of mercury.Codex Committee on Food of European Union has tightened up to meat lead and cadmium maximum residual level
Requirement, be 0.1 and 0.05mg/kg respectively.This experiment uses《Pollutants in food is limited the quantity》(GB2762-2012) limitation
Standard is evaluated collecting data.
As shown in figure 5, test group cadmium content in lean meat is 4 ± 0.25 μ g/Kg, far below standard GB/T 2762-
2012 (≤100 μ g/kg), compared with control group I (conventional material group) and control group II (material containing the high zinc of high-copper and antibiotic),
Heavy metal cadmium in lean meat reduces by 125% and 150% respectively；
Lead Toxicity effect is mainly manifested in the infringement to nervous system, may occur in which higher nerve dysfunction, late period in early days
Encephalosis and neural paralysis can then be caused.Common are simulation learning difficulty, the decline of spatial synthesis ability, ataxia,
The symptoms such as more dynamic, absent minded, aggressive enhancings and IQ decline.Lead can also be by substituting calcium ion or interference calcium ion
Function, to influence the Information Conduction of normal osteocyte, disturb the function of osteocyte.Test group in lean meat lead content be 31 ±
1.2 μ g/Kg, far below standard GB/T 2762-2012 (≤200 μ g/kg), compared with control group I and control group II, thin
Heavy metal lead in meat reduces by 103% times and 135% respectively；
Test group mercury content in lean meat is 1.8 ± 0.3 μ g/Kg, far below standard GB/T 2762-2012 (≤50 μ
G/kg), compared with control group I and control group II, the heavy metal Hg in lean meat reduces by 22.3%% and 44.5% respectively；
Test group arsenic content in lean meat is 8 ± 0.8 μ g/Kg, far below standard GB/T 2762-2012 (≤500 μ g/
Kg), compared with control group I and control group II, the heavy metal arsenic in lean meat reduces 105% and 175% respectively；
Test group copper content in lean meat is 1.53 ± 0.53mg/Kg, far below national standard《Copper limitation health in food
Standard》Copper (≤10mg/kg) in (GB 15199-1994) meat, compared with control group I and control group II, the weight in lean meat
Metallic copper reduces 128% and 305% (P respectively<0.05).
6th, heavy-metal residual analysis of the growing-finishing pig in pork liver
Cadmium is mainly combined storage after being absorbed by animal body with metallothionein, and in liver, kidney and bone, metabolism is slower,
The tissues such as spleen, pancreas, thyroid gland, hair also have certain accumulation.Cadmium has obvious toxic action to growth of animal, long-term intake
The cadmium of low concentration or by the forage grass of cadmium pollution and drinking-water, will cause chronic cadium poisoning.Clinical signs are with skeleton pain, bone
Folding, albuminuria and hepatosis etc..
As shown in fig. 6, test group (growing and fattening pigs green ecological material of the present invention) is not added with inorganic metal in basal diet, examination
Existing test group cadmium, lead, mercury, arsenic, content of copper in lean meat is issued after examination and approval to be substantially less than and control group I (conventional material group) and control group II
(material containing the high zinc of high-copper and antibiotic), and it is far below national standard《In GB2762-2012 national food safety standard food
Pollutant is limited the quantity》The requirement of middle heavy metal content.
Preferred embodiments of the present invention are the foregoing is only, not thereby limit embodiments of the present invention and protection model
Enclose, to those skilled in the art, should can appreciate that all with made by description of the invention and diagramatic content
Scheme obtained by equivalent substitution and obvious change, should be included in protection scope of the present invention.
Claims (6)
1. a kind of green non-pollution fattens pig premix, it is characterised in that is made up of the raw material of following proportionings：
Lactobacterium acidophilus are more than 0.5 trillion cfu；
Enterococcus faecalis is more than 0.3 trillion cfu；
Clostridium butyricum is more than 0.15 trillion cfu；
4.8~6.2kg of salt；
6.2~10.5kg of calcium monohydrogen phosphate；
8.8~12.6kg of calcium carbonate；
245~355g of biological polyoses；
The IU of vitamin A 17.0~19.0 ten thousand；
The IU of vitamine D3 2.8~3.2 ten thousand；
420~460mg of nicotinic acid；
220~228mg of calcium pantothenate；
20kg is supplemented to powdered rice hulls, is the green non-pollution fattening pig premix of 2% addition after being well mixed.
2. green non-pollution according to claim 1 fattens pig premix, it is characterised in that by the raw material group of following proportionings
Into：
Lactobacterium acidophilus are more than 0.5 trillion cfu；
Enterococcus faecalis is more than 0.6 trillion cfu；
Clostridium butyricum is more than 0.15 trillion cfu；
Salt 6.2kg；
Calcium monohydrogen phosphate 10.5kg；
Calcium carbonate 12.6kg；
Biological polyoses 245g；
The IU of vitamin A 18.0 ten thousand；
The IU of vitamine D3 3.0 ten thousand；
Nicotinic acid 440mg；
Calcium pantothenate 224mg；
20kg is supplemented to powdered rice hulls, is the green non-pollution fattening pig premix of 2% addition after being well mixed.
3. green non-pollution according to claim 1 fattens pig premix, it is characterised in that by the raw material group of following proportionings
Into：
Lactobacterium acidophilus are more than 1.2 trillion cfu；
Enterococcus faecalis is more than 0.3 trillion cfu；
Clostridium butyricum is more than 0.25 trillion cfu；
Salt 4.8kg；
Calcium monohydrogen phosphate 6.2kg；
Calcium carbonate 8.8kg；
Biological polyoses 335g；
The IU of vitamin A 18.0 ten thousand；
The IU of vitamine D3 3.0 ten thousand；
Nicotinic acid 440mg；
Calcium pantothenate 224mg；
20kg is supplemented to powdered rice hulls, is the green non-pollution fattening pig premix of 2% addition after being well mixed.
A kind of 4. preparation method of green non-pollution fattening pig premix as described in claim 1-3, it is characterised in that including
Following steps：
(1), according to proportioning, raw material calcium monohydrogen phosphate, calcium carbonate, salt are weighed, calcium monohydrogen phosphate, calcium carbonate, salt are mixed
Mixture carrier one uniformly is obtained, it is standby；
(2) raw material biological polyoses, are weighed, are stirred required vitamin A, vitamine D3, nicotinic acid, calcium pantothenate further according to proportioning
It is well mixed, it is put into mixing plant container and stirs together with the 0.1-50% of the mixture carrier one then obtained with step (1)
Mixing, mix 5~25 minutes, obtain mixture carrier two；
(3), lactobacterium acidophilus, enterococcus faecalis, the living bacteria count of clostridium butyricum according to needed for proportioning, weigh corresponding bacterium powder,
It is put into mixing plant container and is stirred together with the 0.1-50% of the mixture carrier one obtained with step (1), is stirred
15~50 minutes, obtain mixture carrier three.
(4), mixture carrier three, the mixture carrier that mixture carrier two that step (2) obtain, step (3) will be obtained again
One surplus by step (2) and step (3) is put into mixing plant container together, is stirred 5~45 minutes, that is, is made green
Color non-polluted growing and fattening pig premix.
5. the preparation method of green non-pollution fattening pig premix according to claim 4, it is characterised in that including as follows
Step：
(1), according to proportioning, raw material calcium monohydrogen phosphate, calcium carbonate, salt are weighed, calcium monohydrogen phosphate, calcium carbonate, salt are mixed
Mixture carrier one uniformly is obtained, it is standby；
(2) raw material biological polyoses, are weighed, are stirred required vitamin A, vitamine D3, nicotinic acid, calcium pantothenate further according to proportioning
It is well mixed, the mixture carrier one then obtained with step (1) 50% together be put into mixing plant container and be stirred,
Mixing 5~25 minutes, obtains mixture carrier two；
(3), lactobacterium acidophilus, enterococcus faecalis, the living bacteria count of clostridium butyricum according to needed for proportioning, weigh corresponding bacterium powder,
It is put into mixing plant container and is stirred together with other the 50% of the mixture carrier one obtained with step (1), is stirred
15~50 minutes, obtain mixture carrier three.
(4), the mixture carrier three that mixture carrier two that step (2) obtain, step (3) obtain will be put into stirring together again
In equipment container, it is stirred 5~45 minutes, that is, green non-pollution fattening pig premix is made.
A kind of 6. application of the green non-pollution fattening pig premix in growing-finishing pigs as described in claim 1-3.
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