CN108013248A - One boar fermentable fiber feed and preparation method thereof - Google Patents
One boar fermentable fiber feed and preparation method thereof Download PDFInfo
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- CN108013248A CN108013248A CN201711463170.3A CN201711463170A CN108013248A CN 108013248 A CN108013248 A CN 108013248A CN 201711463170 A CN201711463170 A CN 201711463170A CN 108013248 A CN108013248 A CN 108013248A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses boar fermentable fiber feed and preparation method thereof.The present invention is used as raw material using wheat bran etc., a variety of probiotics compatibilities such as saccharomycete, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and lactobacillus reuteri are selected as fermenting microbe, using multiple bacteria compound fermentation technique, pass through the catabolism of microorganism, content of toxins in fibrous raw material can be reduced, protein content in fibrous raw material can be improved, improves the nutritive value of fibrous raw material.Secondly, the present invention selects a variety of probiotics compatibilities as raw materials such as fermenting microbe, wheat brans after solid state fermentation, and the probiotics that can largely improve animal intestinal tract health is contained in biological feedstuff, the micro-ecological environment of intestines and stomach can be improved after biological feedstuff feeding animals, improve the immunocompetence of body.In addition, rich in lactic acid and there is strong alcohol fragrance in biological feedstuff, feed palatability can be significantly improved, is conducive to improve animal feed intake, promotes fast growth of animal, cultivation industry production capacity can be improved.
Description
Technical field
The invention belongs to field of feed, and in particular to boar fermentable fiber feed and preparation method thereof.
Background technology
Contain a large amount of symbiotic microorganisms in animal intestinal tract, they are healthy closely bound up with animal, they participate in many heavy
The physiological function wanted, such as the digestion and metabolism of food, helps host to resist infection etc..For enteric bacteria, fill
The dietary fiber of foot is to safeguard that intestinal flora balance and promotion enteric microorganism play the key of their physiological functions.Dietary fiber
Refer to not be the polysaccharide carbohydrate of animal digestion and the general name of lignin.Most of dietary fiber is not easy to be inhaled by animal
Receive and utilize, but the significant energy source of this part of the food really enteric microorganism.Studies have shown that takes in dietary fiber amount as host
When insufficient, intestinal flora can corrode slime layer in enteron aisle to obtain nutriment, gut barrier function be destroyed, to the health of host
Generation seriously affects.
Feed fibre is primarily referred to as generally being not easy the cell for by the food nutrition thing of animal digestion, mostling come from plant
Wall, includes cellulose, hemicellulose, natural gum, pectin and lignin etc..Fiber is a kind of complicated mixture, water-soluble according to its
The difference of property can be divided into water-soluble fibre (SDF) and water-insoluble fiber (IDF)；Can be divided into again according to its fermenting property difference can
Fermentable fiber and can not fermentable fiber.Wheat bran is the principal by product of wheat flour factory processing, rich in cellulose and
Hemicellulose, while also containing components such as partially protein, fat, oligosaccharide.Crude fiber content in wheat bran is up to 18%, small
Crude fibre in wheat bran skin mainly includes cellulose and hemicellulose, wherein main functional component dietary fiber, containing about 40%.
Wheat bran is a kind of traditional feedstuff, and wheat bran etc. is added in sow feed, can alleviate or solve prevention of sow constipation symptom.One
Aspect is conducive to enteric microorganism using raw materials such as wheat brans and breeds, and microbial population of animal intestinal tract balance is kept, so as to safeguard animal intestinal tract
Health；But then using wheat bran there is also many security risks, the raw material mycotoxin contamination such as wheat bran is serious at present, right
Animal body health causes to seriously affect.Due to the pollution of mycotoxin, cause this good fibrous raw material of wheat bran in herding
The application of cultivation industry is extremely restricted.
Healthy and safe in order to ensure animal, mycotoxin of effectively degrading just becomes industry focus of attention.But, it is traditional
Physics, chemistry removes the method for mycotoxin there are effect is unstable, nutrient component damages are big, influences feed palatability and difficulty
The shortcomings of with large-scale production.Such as the physical adsorption process such as activated carbon method, silicate method, it is while absorbing mycotoxin
Also substantial amounts of vitamin and amino acid have been adsorbed, application effect is poor and additive amount is too big, and occupancy forage compounding ratio is excessive, because
And it is difficult to be widely applied in actual production.And biodegradation technique can be reached brokenly by microorganism and detoxifying function
The effect of bad toxophore, becomes the hot spot of industry concern in recent years.Fermentative feedstuff of microbe is quickly grown in recent years, especially with
The fast development of biotechnology, is made internal disorder or usurp and using just using biofermentation technique to produce fermented feed and grinding for feed addictive
Increasingly arouse people's interest.Fermented feed is more at present to carry out feedstuff using multi-cultur es mixed solid fermentation fermentation method
Processing.Feedstuff, by the catabolism of microorganism, can reduce anti-nutritional factors even toxin in raw material through microbiological treatment
Content；Feedstuff can effectively improve feed nutritive value after microbial fermentation, can also improve prebiotic bacterial content in feed,
Improve the micro-ecological environment of animal gastrointestinal tract, improve the immunocompetence of body.Feed solids fermentation in would generally inoculation yeast bacterium,
It can make fermented feed that there is strong mellowness, significantly improve its palatability, be conducive to improve animal feed intake, improve cultivation row
Industry production capacity.
The content of the invention
The shortcomings that in order to overcome the prior art, is to provide a boar fermentable fiber with deficiency, primary and foremost purpose of the invention
Feed.
Preparation method another object of the present invention is to above-mentioned pig with fermentable fiber feed.
The present invention improves the security and its application range of product on the premise of bran product nutritive value is ensured.
The purpose of the present invention is achieved through the following technical solutions：
One boar fermentable fiber feed, by fermenting to obtain including the following raw material：
The solid-liquid ratio of fermentation base-material and the seed mixture liquid that ferments is 1：0.7~1：1；
The fermentation base-material includes the wheat bran 80%~100% of mass percent meter, skin of beancurd 0~20%.
The fermentation seed mixture liquid include saccharomycete, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and
Lactobacillus reuteri；
Each strain content is in the fermentation seed mixture liquid：Saccharomycete 1 × 107~5 × 107Cfu/mL, preferred concentration
For 2 × 107cfu/mL；Bacillus subtilis 1 × 107~5 × 107Cfu/mL, preferred concentration are 2 × 107cfu/mL；Lichens bud
Spore bacillus 1 × 107~5 × 107Cfu/mL, preferred concentration are 2 × 107cfu/mL；Lactobacillus plantarum 1 × 107~3 × 107cfu/
ML, preferred concentration are 2 × 107cfu/mL；Lactobacillus reuteri 1 × 107~3 × 107Cfu/mL, preferred concentration for 2 ×
107cfu/mL。
Preferably, the saccharomycete is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can yeast for animal feeds bacterium,
Including Saccharomyces Cerevisiae in S accharomyces cerevisiae NKY1.Wherein, Saccharomyces cerevisiae NKY1
In patent " 201410234358.0, a kind of feeding thermostable microcapsule yeast probiotics preparation and preparation method and application "
It is open.
Preferably, the bacillus subtilis is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding
Bacillus subtilis.
Preferably, the bacillus licheniformis is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding
Bacillus licheniformis.
Preferably, the lactobacillus plantarum is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding plant
Thing lactobacillus.
Preferably, the lactobacillus reuteri Ministry of Agriculture promulgates《Catalogue of feed additive varieties》In can be feeding sieve
Yi Shi lactobacillus；Including Lactobacillus reuteri LR1 bacterial strains.Wherein, Lactobacillus reuteri LR1 exist
Disclosed in patent " 201510751130.3, one plants of L. reuteri strains with prebiotic characteristics and its application ".
The one boar preparation method of fermentable fiber feed, includes the following steps：
(1) ferment base-material
Select without mouldy, free from extraneous odour and without rotten wheat bran and soybean skin；Amount of wheat bran is 80%~100%, and skin of beancurd is used
Measure as 0~20%；Raw material is uniformly mixed according to formula, obtains fermentation base-material；
(2) fermentation seed mixture liquid is prepared
By the saccharomycete activated (Saccharomyces cerevisiae), bacillus subtilis (Bacillus
Subtilis), bacillus licheniformis (Baclicus lincheniformis), lactobacillus plantarum (Lactobacillus
Plantarum) and lactobacillus reuteri (Lactobacillus reuteri) strain additive amount is poured into nutrient solution on demand
In, it is thoroughly mixed uniformly, nutrient solution water temperature is controlled at 30 ± 2 DEG C, and it is the seed mixture liquid that ferments that this, which mixes bacterium solution,；
Each strain content is in the fermentation seed mixture liquid：Saccharomycete 1 × 107~5 × 107Cfu/mL, preferred concentration
For 2 × 107cfu/mL；Bacillus subtilis 1 × 107~5 × 107Cfu/mL, preferred concentration are 2 × 107cfu/mL；Lichens bud
Spore bacillus 1 × 107~5 × 107Cfu/mL, preferred concentration are 2 × 107cfu/mL；Lactobacillus plantarum 1 × 107~3 × 107cfu/
ML, preferred concentration are 2 × 107cfu/mL；Lactobacillus reuteri 1 × 107~3 × 107Cfu/mL, preferred concentration for 2 ×
107cfu/mL。
The mass percent meter of component contained by the nutrient solution is as follows：Glucose or sucrose 2%, K2HPO40.3% He
KH2PO40.2%.
Preferably, the saccharomycete is yeast for animal feeds bacterium, including Saccharomyces cerevisiae NKY1 bacterium
Strain.
Preferably, the bacillus subtilis is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding
Bacillus subtilis.
Preferably, the bacillus licheniformis is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding
Bacillus licheniformis.
Preferably, the lactobacillus plantarum is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding plant
Thing bacillus.
Preferably, the lactobacillus reuteri is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding
Lactobacillus reuteri；Including Lactobacillus reuteri LR1 bacterial strains.
(3) base-material ferments
The fermentation seed mixture liquid described in step (2) is uniformly sprayed into the fermentation base-material described in step (1), controls feed liquid
Than 1：0.7~1：1, fermentation base-material is sufficiently mixed uniformly with fermentation seed mixture liquid, and the fermentation base-material being inoculated with is encased in
In fermentation bag, sack is sealed, material tiling thickness of feed layer is 10~15cm, and ferment 36~48h under the conditions of being placed in 30~35 DEG C, obtains
To pig fermentable fiber feed.
Preferably, the fermentation bag is band one-way ventilating valve microbiological anaerobic fermentation bag.
Preferably, it is as follows to prepare situation for the activation of the saccharomycete described in step (2)：
The active dry yeast bacterium for weighing a certain amount of known viable count is added in yeast activated liquid in 2% (m/v) ratio and (contains
0.9%NaCl and 2% glucose), 30 DEG C of 30~40min of concussion activation.
Preferably, it is as follows to prepare situation for the activation of the bacillus subtilis described in step (2)：
The bacillus subtilis powder for weighing a certain amount of known viable count is added in activating solution in 2% (m/v) ratio and (contains
0.9%NaCl and 2% glucose), 37 DEG C of 30~40min of concussion activation.
Preferably, it is as follows to prepare situation for the activation of the bacillus licheniformis described in step (2)：
The bacillus licheniformis powder for weighing a certain amount of known viable count is added in activating solution in 2% (m/v) ratio and (contains
0.9%NaCl and 2% glucose), 37 DEG C of 30~40min of concussion activation.
Preferably, it is as follows to prepare situation for the activation of the lactobacillus plantarum described in step (2)：
Lactobacillus plantarum is seeded to progress level-one culture in MRS fluid nutrient mediums, after 37 DEG C are cultivated 24h, is connect according to 5%
Kind amount carries out two level and spreads cultivation.
Preferably, it is as follows to prepare situation for the activation of the lactobacillus reuteri described in step (2)：
By lactobacillus reuteri be seeded to MRS fluid nutrient mediums (casein peptone 10g, beef extract powder 10g, glucose 20g,
Yeast extract 5g, dipotassium hydrogen phosphate 2g, sodium citrate 2g, epsom salt 0.58g, four water manganese sulfate 0.25g, sodium acetate
5g, Tween-80 1mL, agar 15g；Mentioned component is miscible in 1000mL water, adjust pH5.5) in carry out level-one culture, 37 DEG C
After cultivating 24h, carry out two level according to 5% inoculum concentration and spread cultivation.
The present invention is had the following advantages relative to the prior art and effect：
Wheat bran is a kind of important feed fibre raw material, since the reasons such as the pollution of mycotoxin cause wheat bran should in feed
Use and be restricted, the present invention, through microbiological treatment, passes through micro- life using the fibrous raw material such as multiple bacteria compound fermentation technique, wheat bran
The catabolism of thing, can reduce content of toxins in fibrous raw material, can improve protein content in fibrous raw material, improve fibrous raw material
Nutritive value.Secondly, the present invention select have the function of obvious prebiotic Saccharomyces cerevisiae NKY1 with
The bacterial strains such as Lactobacillus reuteri LR1 as raw materials such as fermenting microbe, wheat brans after solid state fermentation, in biological feedstuff
Containing the probiotics that can largely improve animal intestinal tract health, the Tiny ecosystem ring of intestines and stomach can be improved after biological feedstuff feeding animals
Border, improves the immunocompetence of body.In addition, the raw material such as inoculation yeast bacterium and lactic acid bacteria, wheat bran is fermented in feed solids fermentation
Lactic acid is rich in afterwards and there is strong alcohol fragrance, can significantly improve feed palatability, is conducive to improve animal feed intake, is promoted dynamic
Thing fast-growth, can improve cultivation industry production capacity.
Brief description of the drawings
Fig. 1 is fiber feedstuff zymotechnique flow chart.
Embodiment
With reference to embodiment and attached drawing, the present invention is described in further detail, but embodiments of the present invention are unlimited
In this.
The zymotechnique flow of the pig fermentable fiber feed of the present invention, as shown in Figure 1.
Embodiment 1
Fresh wheat bran 5kg is weighed first as fermentation base-material, is then prepared and is contained 2 × 107Cfu/mL saccharomycete
NKY1、2×107Cfu/mL bacillus subtilises, 2 × 107Cfu/mL bacillus licheniformis, 2 × 107Cfu/mL lactobacillus plantarums,
2×107The mixed bacteria liquid 5.0L of cfu/mL lactobacillus reuteris LR1, stirs evenly, and mixed bacteria liquid is sprayed onto bran feedstock
In, feed liquid is sufficiently mixed uniformly, and the base-material that ferments after inoculation is fitted into band one-way ventilating valve microbiological anaerobic fermentation bag, heat-sealable
Mouthful, fermentation bag is placed in 32 DEG C of environment and is left to ferment 48h, obtains pig fermentable fiber feed.Material sampling difference after fermentation
The viable count (colony counting method) of bacillus, lactobacillus and saccharomycete is measured, material pH value, lactic acid content, mycotoxin contain
Amount, protein content, the results are shown in Table 1.
Indexs measure
A) count plate：Take the wet pigs of 3g to be placed in fermentable fiber feed in 100mL sterile salines, shake 20min,
Take supernatant to be diluted with sterile water, take the dilution of suitable multiple to be respectively coated LB, YPD (addition ampicillin and streptomysin)
Count plate is carried out with MRS tablets.LB tablets testing result is bacillus number；YPD tablets are yeast count；On MRS tablets
It is lactobacillus bacterium number to count gained total bacteria count and subtract bacterium number on LB tablets and YPD tablets.
B) pH value measures：Take the wet pigs of 10g to be placed in fermentable fiber feed in 100mL distilled water, shake 10min, take
Clear liquid surveys pH value with pH meter.
C) lactic acid content measures：Take 10g drying pigs to be placed in fermentable fiber feed in 100mL distilled water, shake 10min,
Centrifuging and taking supernatant builds up the kit detection of biotech firm's offer using Nanjing.
D) vomitoxin measures：Using the mycotoxin detection kit detection of Romer Labs companies.
E) determining the protein quantity：Pig is sampled using Kjeldahl nitrogen determination after drying with fermentable fiber feed.
Table 1 ferments the index of correlation data of front and rear material
| Index | Before fermentation | After fermentation |
| Protein content (%, dry matter meter) | 14.68% | 15.90% |
| Wet stock pH value | 6.67 | 4.11 |
| Lactic acid content (%) | 0 | 1.44% |
| Vomitoxin (ppm) | 1.47 | 0.82 |
| Bacillus (cfu/g) | / | 2.4×106 |
| Saccharomycete (cfu/g) | / | 1.2×107 |
| Lactobacillus (cfu/g) | / | 2.5×109 |
According to table 1, illustrate that the fermented rear protein content of wheat bran improves, under vomitoxin content has by a relatively large margin
Drop, and contain greater number of various probiotics in fermented feed.
Embodiment 2
Fresh 4.5kg wheat brans and 0.5kg skin of beancurd are weighed, is uniformly mixed.Then prepare and contain 2 × 107Cfu/mL ferment
Female bacterium NKY1,2 × 107Cfu/mL bacillus subtilises, 2 × 107Cfu/mL bacillus licheniformis, 2 × 107Cfu/mL plants breast
Bacillus, 2 × 107The mixed bacteria liquid 5.0L of cfu/mL lactobacillus reuteris LR1, stirs evenly.Mixed bacteria liquid is sprayed onto wheat bran
In raw material, feed liquid is sufficiently mixed uniformly, the base-material that ferments after inoculation is fitted into band one-way ventilating valve microbiological anaerobic fermentation bag, heat
Mouth is sealed, fermentation bag is placed in 32 DEG C of environment and is left to ferment 48h, obtains pig fermentable fiber feed.Material takes after fermentation
Sample using measure respectively bacillus, lactobacillus and saccharomycete viable count (colony counting method), material pH value, lactic acid content,
Mycotoxin content, protein content, the results are shown in Table 2.Each Indexs measure is with reference to embodiment 1.
Table 2 ferments the index of correlation data of front and rear material
| Index | Before fermentation | After fermentation |
| Protein content (dry matter) | 14.23% | 15.41% |
| Material pH value | 6.64 | 4.14 |
| Lactic acid content | 0 | 1.66% |
| Vomitoxin | 1.36 | 0.75 |
| Bacillus | / | 1.6×106 |
| Saccharomycete | / | 1.4×107 |
| Lactobacillus | / | 2.3×109 |
According to table 2, illustrate that the fermented rear protein content of wheat bran improves, under vomitoxin content has by a relatively large margin
Drop, and contain greater number of various probiotics in fermented feed.
Embodiment 3
Fresh 4.0kg wheat brans and 1.0kg skin of beancurd are weighed, is uniformly mixed.Then prepare and contain 2 × 107Cfu/mL ferment
Female bacterium NKY1,2 × 107Cfu/mL bacillus subtilises, 2 × 107Cfu/mL bacillus licheniformis, 2 × 107Cfu/mL plants breast
Bacillus, 2 × 107The mixed bacteria liquid 5.0L of cfu/mL lactobacillus reuteris LR1, stirs evenly.Mixed bacteria liquid is sprayed onto wheat bran
In raw material, feed liquid is sufficiently mixed uniformly, the base-material that ferments after inoculation is fitted into band one-way ventilating valve microbiological anaerobic fermentation bag, heat
Mouth is sealed, fermentation bag is placed in 32 DEG C of environment and is left to ferment 48h, obtains pig fermentable fiber feed.Material takes after fermentation
Sample using measure respectively bacillus, lactobacillus and saccharomycete viable count (colony counting method), material pH value, lactic acid content,
Mycotoxin content, protein content, the results are shown in Table 3.Each Indexs measure is with reference to embodiment 1.
Table 3 ferments the index of correlation data of front and rear material
| Index | Before fermentation | After fermentation |
| Protein content (%) | 13.69 | 14.70 |
| Material pH value | 6.65 | 4.13 |
| Lactic acid content | 0 | 1.57% |
| Vomitoxin | 1.25 | 0.88 |
| Bacillus | / | 1.2×106 |
| Saccharomycete | / | 8.3×106 |
| Lactobacillus | / | 1.8×109 |
According to table 3, illustrate that the fermented rear protein content of wheat bran improves, under vomitoxin content has by a relatively large margin
Drop, and contain greater number of various probiotics in fermented feed.
Embodiment 4
Fresh wheat bran 20kg, then prepares and contains 2 × 107Cfu/mL saccharomycete NKY1,2 × 107Cfu/mL withered grass buds
Spore bacillus, 2 × 107Cfu/mL bacillus licheniformis, 2 × 107Cfu/mL lactobacillus plantarums, 2 × 107Cfu/mL Luo Yishi breast bars
The mixed bacteria liquid 20.0L of bacterium LR1, stirs evenly.Mixed bacteria liquid is sprayed onto in bran feedstock, feed liquid is sufficiently mixed uniformly, will
Fermentation base-material after inoculation is fitted into band one-way ventilating valve microbiological anaerobic fermentation bag, heat sealing, and fermentation bag tiling is placed in
48h is left to ferment in 32 DEG C of environment, thickness of feed layer is not higher than 15cm, obtains pig fermentable fiber feed.Material takes after fermentation
Sample measures the viable count (colony counting method) of bacillus, lactobacillus and saccharomycete respectively, material pH value, mycotoxin content,
Protein content, the results are shown in Table 4.Each Indexs measure is with reference to embodiment 1.Material can be directly appended to feed pig in feed after fermentation,
Or used after drying as feedstuff.
Table 4 ferments the index of correlation data of front and rear material
| Index | Before fermentation | After fermentation |
| Albumen (%) | 14.68% | 15.72% |
| Material pH value | 6.60 | 4.15 |
| Lactic acid content | 0 | 1.48% |
| Mycotoxin | 1.47 | 0.86 |
| Bacillus | / | 2.2×106 |
| Saccharomycete | / | 1.5×107 |
| Lactobacillus | / | 2.2×109 |
According to table 4, illustrate that the fermented rear protein content of wheat bran improves, under vomitoxin content has by a relatively large margin
Drop, and contain greater number of various probiotics in fermented feed.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (8)
1. boar fermentable fiber feed, it is characterised in that by fermenting to obtain including the following raw material：
The solid-liquid ratio of fermentation base-material and the seed mixture liquid that ferments is 1：0.7~1：1；
The fermentation base-material includes the wheat bran 80%~100% of mass percent meter, skin of beancurd 0~20%；
The fermentation seed mixture liquid includes saccharomycete, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and Roy
Family name's lactobacillus.
2. pig according to claim 1 fermentable fiber feed, it is characterised in that：
Each strain content is in the fermentation seed mixture liquid：Saccharomycete 1 × 107~5 × 107cfu/mL；Bacillus subtilis
1×107~5 × 107cfu/mL；Bacillus licheniformis 1 × 107~5 × 107cfu/mL；Lactobacillus plantarum 1 × 107~3 ×
107cfu/mL；Lactobacillus reuteri 1 × 107~3 × 107cfu/mL。
3. pig according to claim 1 or 2 fermentable fiber feed, it is characterised in that：
Each strain content is in the fermentation seed mixture liquid：Saccharomycete 2 × 107cfu/mL；Bacillus subtilis 2 ×
107cfu/mL；Bacillus licheniformis 2 × 107cfu/mL；Lactobacillus plantarum 2 × 107cfu/mL；Lactobacillus reuteri 2 ×
107cfu/mL。
4. the boar preparation method of fermentable fiber feed, it is characterised in that include the following steps：
(1) ferment base-material
Select without mouldy, free from extraneous odour and without rotten wheat bran and soybean skin；Amount of wheat bran is 80%~100%, and skin of beancurd dosage is 0
~20%；Raw material is uniformly mixed according to formula, obtains fermentation base-material；
(2) fermentation seed mixture liquid is prepared
By the saccharomycete activated (Saccharomyces cerevisiae), bacillus subtilis (Bacillus
Subtilis), bacillus licheniformis (Baclicus lincheniformis), lactobacillus plantarum (Lactobacillus
Plantarum) and lactobacillus reuteri (Lactobacillus reuteri) strain additive amount is poured into nutrient solution on demand
In, it is thoroughly mixed uniformly, nutrient solution water temperature is controlled at 30 ± 2 DEG C, and it is the seed mixture liquid that ferments that this, which mixes bacterium solution,；
(3) base-material ferments
The fermentation seed mixture liquid described in step (2) is uniformly sprayed into the fermentation base-material described in step (1), control solid-liquid ratio exists
1：0.7~1：1, fermentation base-material is sufficiently mixed uniformly with fermentation seed mixture liquid, and the fermentation base-material being inoculated with is encased in fermentation
In bag, sack is sealed, material tiling thickness of feed layer is 10~15cm, and ferment 36~48h under the conditions of being placed in 30~35 DEG C, obtains pig
With fermentable fiber feed.
5. the pig according to claim 4 preparation method of fermentable fiber feed, it is characterised in that：
In step (2), each strain content is in the fermentation seed mixture liquid：Saccharomycete 1 × 107~5 × 107cfu/mL；It is withered
Careless bacillus 1 × 107~5 × 107cfu/mL；Bacillus licheniformis 1 × 107~5 × 107cfu/mL；Lactobacillus plantarum 1 ×
107~3 × 107cfu/mL；Lactobacillus reuteri 1 × 107~3 × 107cfu/mL。
6. the pig according to claim 4 or 5 preparation method of fermentable fiber feed, it is characterised in that：
In step (2), each strain content is in the fermentation seed mixture liquid：Saccharomycete 2 × 107cfu/mL；Bacillus subtilis
Bacterium 2 × 107cfu/mL；Bacillus licheniformis 2 × 107cfu/mL；Lactobacillus plantarum 2 × 107cfu/mL；Lactobacillus reuteri 2 ×
107cfu/mL。
7. the pig according to claim 4 or 5 preparation method of fermentable fiber feed, it is characterised in that：
In step (2), the mass percent meter of component contained by the nutrient solution is as follows：Glucose or sucrose 2%, K2HPO4
0.3% and KH2PO40.2%.
8. the pig according to claim 4 or 5 preparation method of fermentable fiber feed, it is characterised in that：
In step (3), the fermentation bag is band one-way ventilating valve microbiological anaerobic fermentation bag.
Priority Applications (1)
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| CN108795820A (en) * | 2018-06-29 | 2018-11-13 | 江苏盐城源耀生物科技有限公司 | It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process |
| CN108850536A (en) * | 2018-05-16 | 2018-11-23 | 安徽志德动物营养科技有限公司 | A kind of the dietary fiber ingredient and its manufacturing method of livestock feed |
| CN109198160A (en) * | 2018-08-31 | 2019-01-15 | 重庆金银卡生物饲料有限公司 | One broad sow concentrated feed and preparation method thereof |
| CN110024910A (en) * | 2019-05-17 | 2019-07-19 | 河南农业大学 | The method for improving wormwood tunning quality with cellulase and composite probiotics ferment |
| CN110050885A (en) * | 2019-05-15 | 2019-07-26 | 北京波尔莱特饲料有限公司 | A kind of fermentable fiber feed and its preparation method and application method |
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| CN110663820A (en) * | 2018-07-02 | 2020-01-10 | 辽宁波尔莱特农牧实业有限公司 | Method for preparing fermented bran through bacterium-enzyme synergistic fermentation |
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| CN110050885A (en) * | 2019-05-15 | 2019-07-26 | 北京波尔莱特饲料有限公司 | A kind of fermentable fiber feed and its preparation method and application method |
| CN110024910A (en) * | 2019-05-17 | 2019-07-19 | 河南农业大学 | The method for improving wormwood tunning quality with cellulase and composite probiotics ferment |
| CN110353088A (en) * | 2019-07-18 | 2019-10-22 | 广东省农业科学院动物科学研究所 | A kind of preparation method of fermented bean dregs |
| CN110226671A (en) * | 2019-07-19 | 2019-09-13 | 广东省农业科学院农业生物基因研究中心 | A kind of piglet full price fermented feed and its production method |
| CN110558420A (en) * | 2019-10-10 | 2019-12-13 | 北京挑战农业科技有限公司 | High-dietary-fiber low-antigen-protein fermented soybean hull |
| CN110973351A (en) * | 2019-12-19 | 2020-04-10 | 广东省农业科学院动物科学研究所 | Calliandra haematocephala fermentation product and preparation method and application thereof |
| CN111493218A (en) * | 2020-03-18 | 2020-08-07 | 广州智特奇生物科技股份有限公司 | Fermented dietary fiber feed raw material and preparation method thereof |
| CN112998123A (en) * | 2021-04-27 | 2021-06-22 | 吉林农业大学 | Fermentation process for improving alcohol soluble protein content of DDGS feed |
| CN114847406A (en) * | 2022-05-26 | 2022-08-05 | 哈七(广东)生物技术有限公司 | Composition for improving antibacterial ability of oregano and preparation method and application thereof |
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| CN116391793B (en) * | 2022-12-01 | 2023-10-24 | 山东中牧兽药有限公司 | Process for microbial fermentation of feed and application |
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Application publication date: 20180511 |