CN108795820A - It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process - Google Patents
It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process Download PDFInfo
- Publication number
- CN108795820A CN108795820A CN201810716122.9A CN201810716122A CN108795820A CN 108795820 A CN108795820 A CN 108795820A CN 201810716122 A CN201810716122 A CN 201810716122A CN 108795820 A CN108795820 A CN 108795820A
- Authority
- CN
- China
- Prior art keywords
- dregs
- mixing
- fermentation
- beans
- probiotics
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000046052 Phaseolus vulgaris Species 0.000 title claims abstract description 43
- 235000010627 Phaseolus vulgaris Nutrition 0.000 title claims abstract description 43
- 239000006041 probiotic Substances 0.000 title claims abstract description 31
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 31
- 238000000855 fermentation Methods 0.000 title claims abstract description 28
- 230000004151 fermentation Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims description 13
- 238000010563 solid-state fermentation Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
- 235000009566 rice Nutrition 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000005862 Whey Substances 0.000 claims description 5
- 102000007544 Whey Proteins Human genes 0.000 claims description 5
- 108010046377 Whey Proteins Proteins 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 235000020183 skimmed milk Nutrition 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims 3
- 241001465754 Metazoa Species 0.000 abstract description 20
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 230000000433 anti-nutritional effect Effects 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 abstract description 5
- 230000003115 biocidal effect Effects 0.000 abstract description 5
- 230000002503 metabolic effect Effects 0.000 abstract description 5
- 230000036542 oxidative stress Effects 0.000 abstract description 5
- 235000013406 prebiotics Nutrition 0.000 abstract description 5
- 240000006024 Lactobacillus plantarum Species 0.000 abstract description 4
- 235000013965 Lactobacillus plantarum Nutrition 0.000 abstract description 4
- 241000186604 Lactobacillus reuteri Species 0.000 abstract description 4
- 235000019621 digestibility Nutrition 0.000 abstract description 4
- 229940072205 lactobacillus plantarum Drugs 0.000 abstract description 4
- 229940001882 lactobacillus reuteri Drugs 0.000 abstract description 4
- 240000006439 Aspergillus oryzae Species 0.000 abstract description 3
- 235000002247 Aspergillus oryzae Nutrition 0.000 abstract description 3
- 244000063299 Bacillus subtilis Species 0.000 abstract description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract description 3
- 239000000047 product Substances 0.000 description 36
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 241000209094 Oryza Species 0.000 description 5
- 238000012795 verification Methods 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 150000003254 radicals Chemical class 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 229920001542 oligosaccharide Polymers 0.000 description 3
- 150000002482 oligosaccharides Chemical class 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000001814 pectin Substances 0.000 description 3
- 229920001277 pectin Polymers 0.000 description 3
- 235000010987 pectin Nutrition 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- -1 element Polymers 0.000 description 2
- 230000007760 free radical scavenging Effects 0.000 description 2
- 230000001408 fungistatic effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001228 trophic effect Effects 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- FRYDSOYOHWGSMD-UHFFFAOYSA-N [C].O Chemical compound [C].O FRYDSOYOHWGSMD-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 229940125532 enzyme inhibitor Drugs 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000003726 plant lectin Substances 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The present invention provides a kind of mixing probiotics for bean pulp fermentation, and the mixing probiotics is mixed by the strain of following parts by weight：3-5 parts of lactobacillus reuteri, 3-5 parts of lactobacillus plantarum, 1-3 parts of bacillus subtilis, 1-3 parts of aspergillus oryzae.The present invention also provides the methods of above-mentioned mixing probiotics fermention dregs of beans.The present invention also provides the fermented products that above-mentioned fermentation process ferments.The present invention for the mixing probiotics of bean pulp fermentation and its fermentation process, can a variety of anti-nutritional factors in fully degraded dregs of beans, be added into the digestibility that animal can be improved in feed to feed；It is also rich in prebiotic thalline and beneficial metabolic product, part antibiotic can be played the role of by being added in feed, while help to alleviate the oxidative stress of swinery, improved animal products quality, improved breeding performonce fo animals and immunocompetence.
Description
Technical field
The present invention relates to feed addictives more particularly to a kind of for the mixing probiotics of bean pulp fermentation and its fermentation side
Method.
Background technology
Dregs of beans becomes widest plant egg because it has more balanced amino acid composition and good nutritive peculiarity
The first choice of protein resource in white matter raw material and feed.But due to containing in dregs of beans, there are many anti-nutritional factors, such as tryptose
Enzyme inhibitor, phytolectin, oligosaccharide and antigen protein etc. limit the use of dregs of beans.
Simultaneously as the improvement of people's living standards, requirement of the people to animal products quality is also higher and higher, reduce anti-
Raw the element even trend for being added to livestock-raising future development of antibiotic-free.
The study found that microorganism can be used for fermented bean dregs, to obtain the fermented product with nutritive value.But it is existing
Zymotechnique used by strain, technique it is different, be primarily present problems with：The nutrition in dregs of beans cannot be made full use of
Ingredient, fermented product have a single function.
Therefore, a kind of microorganism formulation can make full use of the nutritional ingredient in dregs of beans is researched and developed, can be raised making an addition to
Feed addictive with more wide variety of functions when in material has a vast market foreground.
Invention content
The technical problem to be solved by the present invention is to：In view of the drawbacks of the prior art, it provides a kind of for bean pulp fermentation
Mix probiotics and its fermentation process, can a variety of anti-nutritional factors in fully degraded dregs of beans, being added into feed can improve
Digestibility of the animal to feed；It is also rich in prebiotic thalline and beneficial metabolic product, part can be played by being added in feed
The effect of antibiotic, at the same contribute to alleviate swinery oxidative stress, improve animal products quality, improve breeding performonce fo animals and
Immunocompetence.
The present invention uses following technical scheme to solve above-mentioned technical problem：
It is an object of the present invention to provide a kind of mixing probiotics for bean pulp fermentation, the mixing probiotics by with
The strain of lower parts by weight mixes：
In order to optimize above-mentioned technical solution, the technical measures that the present invention takes further include：
Further, the mixing probiotics is mixed by the strain of following parts by weight：
It is a further object to provide the methods of above-mentioned mixing probiotics fermention dregs of beans, by dregs of beans and corn, rice
After the chaff dregs of rice, skimmed milk power, whey powder and glucose mixing, it is 1.5 that a certain amount of water, which is added, and adjusts material-water ratio:1 (material：Water),
It is used as solid-state fermentation culture medium after mixing, sterilizing, mixing probiotics is seeded in solid-state hair according to the weight ratio of 4%-10%
In ferment culture medium, fermentation time 45-60h.
Further, the weight percent of each component is in the solid-state fermentation culture medium：Dregs of beans 15%-30%, corn
20%-60%, rice bran meal 5%-15%, skimmed milk power 5%-20%, whey powder 2%-15%, glucose 1%-10%.
Further, the mixing probiotics is seeded in solid-state fermentation culture medium according to 4%~10% weight ratio.
Further, the solid-state fermentation culture medium after fermentation is dry, pulverize and sieve, sieve is 40-60 mesh, must both be fermented
Product.Preferably cross 40 mesh sieve.
It is a further object to provide the fermented products that above-mentioned fermentation process ferments.
Lactobacillus reuteri can be colonized in enteron aisle, inhibited pathogenic entero becteria breeding, improved immune function, reduce animal oxygen
Change stress, reduce animal disease, adjust intestinal flora distribution, and lactobacillus reuteri can generate a kind of the wide of non-protein class
Compose antibacterial substance Luo Yishi bacteriocins；Lactobacillus plantarum not only acts as the effect of leavening, moreover it is possible to generate wind during the fermentation
Taste substance improves the palatability of feed, while lactobacillus plantarum also has the function of alleviating oxidative stress, so as to promote pig
The health and improvement animal products quality of group；Bacillus subtilis secretes multiple protein enzyme in growth course can degrade dregs of beans
In macro-molecular protein include antigen protein, make it become to be conducive to the peptides digested and assimilated and amino acid；Aspergillus oryzae fermented
Generation enzyme related with carbon hydrate can be metabolized in journey, cellulose, hemicellulose, starch and pectin etc. in raw material of degrading
Macromolecular substances, so as to cause thick protein, the relative increase of total energy and fat.Strain combination is matched during the present invention uses above-mentioned 4
5, it cooperates between each strain, cooperative fermentation, using metabolic characteristic possessed by various probiotics, for fermenting with dregs of beans,
The anti-nutritional factors that can not only be degraded in dregs of beans, additionally it is possible to fully decompose high molecular weight protein in dregs of beans, cellulose, hemicellulose
The macromolecular substances such as element, starch and pectin assign dregs of beans new trophic function, obtain it is full of nutrition and with bacteria resistance function,
The fermented product of good mouthfeel.
The present invention is had the following technical effect that compared with prior art using above-mentioned technical proposal：
The raw materials such as mixing probiotic combinations fermented bean dregs of the present invention, can not only a variety of anti-nutrition in fully degraded dregs of beans
The factor is added into the digestibility that animal can be improved in feed to feed；Also make product rich in prebiotic thalline and beneficial to generation
Thank to product, fermented product, which is added in feed, can play the role of part antibiotic, while help to alleviate the oxidation of swinery
Stress, improve animal products quality, improve breeding performonce fo animals and immunocompetence, the comprehensive utilization for improving dregs of beans has weight
The meaning wanted.
Description of the drawings
Fig. 1 is the fungistatic effect schematic diagram that probiotics fermention product is mixed in embodiment 2 to k88.
Specific implementation mode
Technical scheme of the present invention is described in further detail with reference to embodiment.Following embodiment is only used for more
Add and clearly demonstrate technical scheme of the present invention, and not intended to limit the protection scope of the present invention.
Embodiment 1
In the present embodiment, lactobacillus reuteri, lactobacillus plantarum, bacillus subtilis and aspergillus oryzae are according to 3:3：2:2
Weight ratio is mixed to get mixing probiotics, and solid-state fermentation culture medium, material-water ratio 1.5 are accessed according to 8% inoculum concentration:1
(material：Water), fermentation time 48h is dry by the solid-state fermentation culture medium after fermentation after fermentation, crushed 40 mesh sieve, obtains
Fermented product.
Wherein, the weight percent of each component is in solid-state fermentation culture medium：Dregs of beans 15%-30%, corn 20%-
60%, rice bran meal 5%-15%, skimmed milk power 5%-20%, whey powder 2%-15%, glucose 1%-10%.
Embodiment 2
The present embodiment is verification embodiment.
The present embodiment verifies the suppression of the mixing probiotics fermention product of the present invention using E.coli K88 as experimental subjects
Bacterium ability.
Weigh made from 1g fermentation process according to the invention that fermented product is in sterilizing test tubes, by 1:2 (m/v) ratios
Sterile water, mixing is added, 3000r/min centrifuges 10min, and supernatant is taken to carry out bacteriostatic experiment to E.coli K88.Bacteriostatic experiment
Each tablet adds 100 μ L of pathogenic bacteria to carry out coated plate, is loaded using Oxford cup, 200 μ L of product are loaded per hole, each pathogenic bacteria is cooked
2 parallel, spreads 1h in 4 DEG C of refrigerators after sample addition, is subsequently placed in 37 DEG C of incubators and cultivates 8h.
Observation is taken out, as a result as shown in Fig. 1.Wherein, 2 parallel group inhibition zones on top be experimental group, 2 of lower part
Parallel group inhibition zone is blank control group.
By experimental result, it can be seen that, fermented product of the invention has apparent good fungistatic effect.Therefore, this is sent out
Bright fermented product, which makes an addition in feed, to inhibit animal enterpathogen to breed, and improve Immune Function In Animals.
Embodiment 3
The present embodiment is verification embodiment.
The variation of dregs of beans nutritional ingredient before and after the mixing probiotics fermention dregs of beans of the present embodiment verification present invention.
The experimental results showed that under same dry matter basis, it is thick in the dregs of beans after the mixing probiotics fermention of the present invention
The more non-fermented bean dregs of albumen, which are compared, improves 5.21%, and reaches 5.81 × 108cfu/g beneficial to bacterium number, and lactic acid content reaches
6.14%；Antigen protein and bad oligosaccharides are respectively by SDS-PAGE electrophoretic analysis and TLC plate analysis, compared to fermentation after fermentation
Before, antigen protein and the degradation of bad oligosaccharides almost all.
The above results show the anti-nutritional factors that mixing probiotics of the invention can not only degrade in dregs of beans, additionally it is possible to
The macromolecular substances such as high molecular weight protein, cellulose, hemicellulose, starch and the pectin in dregs of beans are fully decomposed, it is new to assign dregs of beans
Trophic function；Rich in prebiotic thalline and beneficial metabolic product, inhibit pathogenic entero becteria breeding, being added in feed can play
The effect of part antibiotic.
Embodiment 4
The present embodiment is verification embodiment.
Free radical scavenging activity (DPPH) be it is a kind of it is artificial synthesized with Single Electron centered on nitrogen
Organic free radical with good stability energy；The present embodiment verifies this hair by the measurement to DPPH Scavenging abilities
The oxidation resistance of bright mixing probiotics fermention product.
The measurement of scavenging ability of DPPH free radical is defined as 1 active unit (U) to 50% clearance rate of DPPH free radicals,
DPPH free radical scavenging abilities indicate (U/g with the active unit contained in the fermented product extracting solution sample of every gram of dry weight
DW)。
Experimental method：After fermented sample crushed 40 mesh sieve, with 50% ethyl alcohol with 1:20 solid-liquid ratios, under the conditions of 55 DEG C
6h is impregnated, 3000r/min centrifuges 10min and obtains supernatant, its ability for removing DPPH free radicals is measured with supernatant.
Experimental result：The fermented product for obtaining the present invention after measured removes the ability of DPPH free radicals as 2124U/g DW.
This example demonstrates that the fermented product of the present invention has stronger oxidation resistance, the fermented product of the present invention is added
It is added on the oxidative stress that can aid in feed and alleviate swinery, improves animal products quality, improves breeding performonce fo animals.
Embodiment 5
The present embodiment is verification embodiment.
Following experimental group and control group is arranged in the present embodiment, verifies work of the fermented product to growth of animal performance of the present invention
With.
Experimental group uses the fermented product that the embodiment of the present invention 1 obtains；
Control group uses a kind of public for the composite flora of bean pulp fermentation and its application institute according to patent CN104762234A
Fermented product prepared by the embodiment 4 for the method opened；
Experimental method：To be tested in Jiangsu source one pork pig farm of credit, experimental period is 2 months；Choose health, body
The approximate pig of weight 100, is randomly divided into 2 groups of experimental group and control group, the fermented product for respectively obtaining experimental group and control group
It is made an addition in pannage according to 10% weight ratio, the incidence of pig during statistical experiment, and the front and back pig of experiment
Weight, the experimental results are shown inthe following table (weight of pig is averaged)：
By upper table result it is found that relative to control group, fermented product of the invention can preferably reduce the morbidity of pig
Rate adjusts the immunocompetence of pig, promotes the growth of pig.
By above-described embodiment it is found that the raw materials such as mixing probiotic combinations fermented bean dregs of the present invention, can not only fully drop
A variety of anti-nutritional factors in dregs of beans are solved, the digestibility that can improve animal in feed to feed is added into；Also make product rich
Containing prebiotic thalline and beneficial metabolic product, fermented product, which is added in feed, can play the role of part antibiotic, have simultaneously
The oxidative stress for helping alleviate swinery, improves animal products quality, improves breeding performonce fo animals and immunocompetence, for improving beans
The comprehensive utilization of the dregs of rice has great importance.
Specific embodiments of the present invention are described in detail above, but it is only used as example, the present invention is not intended to limit
In particular embodiments described above.To those skilled in the art, it is any to the practicality carry out equivalent modifications and replace
In generation, is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by impartial conversion and repair
Change, all should be contained within the scope of the invention.
Claims (7)
1. a kind of mixing probiotics for bean pulp fermentation, which is characterized in that the mixing probiotics is by following parts by weight
Strain mixes：
2. mixing probiotics according to claim 1, which is characterized in that the mixing probiotics is by following parts by weight
Strain mixes：
3. the method for mixing probiotics fermention dregs of beans according to claim 1, which is characterized in that by dregs of beans and corn, rice
After the chaff dregs of rice, skimmed milk power, whey powder and glucose mixing, it is 1.5 that a certain amount of water, which is added, and adjusts material-water ratio:1 (material：Water),
It is used as solid-state fermentation culture medium after mixing, sterilizing, mixing probiotics is seeded in solid-state hair according to the weight ratio of 4%-10%
In ferment culture medium, fermentation time 45-60h.
4. according to the method described in claim 3, it is characterized in that, in the solid-state fermentation culture medium each component weight percent
Than for：Dregs of beans 15%-30%, corn 20%-60%, rice bran meal 5%-15%, skimmed milk power 5%-20%, whey powder 2%-
15%, glucose 1%-10%.
5. according to the method described in claim 3, it is characterized in that, described mix weight ratio of the probiotics according to 4%-10%
It is seeded in solid-state fermentation culture medium.
6. according to the method described in claim 3, it is characterized in that, the solid-state fermentation culture medium after fermentation is dry, crushed
Sieve, sieve are 40-60 mesh to get fermented product.
7. the fermented product that the method according to any one of claim 3-6 is fermented.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810716122.9A CN108795820A (en) | 2018-06-29 | 2018-06-29 | It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810716122.9A CN108795820A (en) | 2018-06-29 | 2018-06-29 | It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108795820A true CN108795820A (en) | 2018-11-13 |
Family
ID=64074205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810716122.9A Pending CN108795820A (en) | 2018-06-29 | 2018-06-29 | It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108795820A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528380A (en) * | 2021-06-28 | 2021-10-22 | 国家粮食和物资储备局科学研究院 | Multi-strain composite brown rice solid-state fermentation microbial inoculum, and preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102885196A (en) * | 2012-09-28 | 2013-01-23 | 漳州大北农农牧科技有限公司 | Microbial fermentation method of soybean meal and application of soybean meal in feed |
| CN103283961A (en) * | 2013-05-10 | 2013-09-11 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
| CN104762234A (en) * | 2015-04-09 | 2015-07-08 | 河南工业大学 | Complex bacterium for bean pulp fermentation and application of complex bacterium |
| CN104996722A (en) * | 2015-08-13 | 2015-10-28 | 江南大学 | Method of two-step united multi-strain fermented feed |
| CN105219683A (en) * | 2015-11-04 | 2016-01-06 | 广东省农业科学院动物科学研究所 | One strain has L. reuteri strain and the application thereof of prebiotic characteristics |
| CN106359904A (en) * | 2016-11-25 | 2017-02-01 | 广东省农业科学院动物科学研究所 | Application of lactobacillus reuteri in replacement of feed antibiotic |
| CN108013248A (en) * | 2017-12-28 | 2018-05-11 | 广东省农业科学院动物科学研究所 | One boar fermentable fiber feed and preparation method thereof |
-
2018
- 2018-06-29 CN CN201810716122.9A patent/CN108795820A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102885196A (en) * | 2012-09-28 | 2013-01-23 | 漳州大北农农牧科技有限公司 | Microbial fermentation method of soybean meal and application of soybean meal in feed |
| CN103283961A (en) * | 2013-05-10 | 2013-09-11 | 浙江工业大学 | Method for preparing health-benefiting fermented soybean meal |
| CN104762234A (en) * | 2015-04-09 | 2015-07-08 | 河南工业大学 | Complex bacterium for bean pulp fermentation and application of complex bacterium |
| CN104996722A (en) * | 2015-08-13 | 2015-10-28 | 江南大学 | Method of two-step united multi-strain fermented feed |
| CN105219683A (en) * | 2015-11-04 | 2016-01-06 | 广东省农业科学院动物科学研究所 | One strain has L. reuteri strain and the application thereof of prebiotic characteristics |
| CN106359904A (en) * | 2016-11-25 | 2017-02-01 | 广东省农业科学院动物科学研究所 | Application of lactobacillus reuteri in replacement of feed antibiotic |
| CN108013248A (en) * | 2017-12-28 | 2018-05-11 | 广东省农业科学院动物科学研究所 | One boar fermentable fiber feed and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王璐: "《微生物快乐学》", 30 August 2016 * |
| 赤峰四海同心生物科技有限公司: "高活性发酵豆粕产业化开发与应用", 《科技成果登记表》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528380A (en) * | 2021-06-28 | 2021-10-22 | 国家粮食和物资储备局科学研究院 | Multi-strain composite brown rice solid-state fermentation microbial inoculum, and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102356822B (en) | Fermented traditional Chinese medicine feed additive for pigs and preparation method thereof | |
| CN110122658A (en) | A kind of fermentative feedstuff of microbe and its preparation method and application | |
| CN106834163A (en) | Feed fermentation agent, fermented feed and preparation method thereof | |
| CN110226671A (en) | A kind of piglet full price fermented feed and its production method | |
| CN104017751B (en) | Bacillus coagulans, Lactobacillus plantarum and Lactobacillus casei preparation and its preparation | |
| CN102018098A (en) | Preparation method for fermented feed for corn-bean pulp type daily ration | |
| CN109846035A (en) | A kind of compound probiotic composition with body weight control | |
| CN103627656A (en) | Solid state fermentation method of mixed bacteria of clostridium butyricum and bacillus coagulans | |
| CN108835444A (en) | Kind goose feed, preparation method, application and fowl food | |
| CN110169545A (en) | A method of fermentation duck is prepared using lactic acid bacteria | |
| CN107348001A (en) | A kind of method that mixed fungus fermentation prepares modified purple perilla dregs of rice powder | |
| CN110122567A (en) | Composite fermentation cream with anti-oxidation function and preparation method thereof | |
| CN108467846A (en) | The cultural method of lactobacillus acidophilus and its application | |
| CN112205536A (en) | Broiler feed and preparation method thereof | |
| CN104824773A (en) | Fresh sweet corn vinegar | |
| CN107410703A (en) | A kind of livestock and poultry protein feed and its production method | |
| CN116849292B (en) | Fermented feed additive and preparation method and application thereof | |
| CN109170275A (en) | Fermented feed and preparation method thereof and the application in improvement constipation | |
| CN101219154A (en) | Microorganism additive agent for improving phylactic power of pets and accelerating growth | |
| CN112471332A (en) | Antibiotic-free suckling pig nursing material containing complex enzyme and preparation method thereof | |
| CN108112800A (en) | One boar food and preparation method thereof | |
| CN108795820A (en) | It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process | |
| KR20210138346A (en) | Method for preparing fermented total mixed ration using microbial strain complex and steam treatment | |
| CN104172104B (en) | A kind of preparation method of Bifidobacterium soy sauce and the product of preparation thereof | |
| CN109805205A (en) | A method of preparing double fermentation type meat Duckling feed additive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 224224 Nanan Industrial Park, nanshenzao Town, Dongtai City, Yancheng City, Jiangsu Province Applicant after: Yuanyao Biotechnology (Yancheng) Co.,Ltd. Address before: 224224 Nanan Industrial Park, nanshenzao Town, Dongtai City, Yancheng City, Jiangsu Province Applicant before: JIANGSU YANCHENG YUANYAO BIOTECHNOLOGY Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181113 |
|
| RJ01 | Rejection of invention patent application after publication |