CN108795820A - It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process - Google Patents
It is a kind of for the mixing probiotics of bean pulp fermentation and its fermentation process Download PDFInfo
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- CN108795820A CN108795820A CN201810716122.9A CN201810716122A CN108795820A CN 108795820 A CN108795820 A CN 108795820A CN 201810716122 A CN201810716122 A CN 201810716122A CN 108795820 A CN108795820 A CN 108795820A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 28
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- 238000000034 method Methods 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims description 13
- 238000010563 solid-state fermentation Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000005862 Whey Substances 0.000 claims description 5
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- 239000008103 glucose Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
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- 230000003115 biocidal effect Effects 0.000 abstract description 5
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- 239000000047 product Substances 0.000 description 36
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
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- 241000588724 Escherichia coli Species 0.000 description 2
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- 150000001413 amino acids Chemical class 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
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- 230000007760 free radical scavenging Effects 0.000 description 2
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- 230000006872 improvement Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
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- 108010062877 Bacteriocins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- FRYDSOYOHWGSMD-UHFFFAOYSA-N [C].O Chemical compound [C].O FRYDSOYOHWGSMD-UHFFFAOYSA-N 0.000 description 1
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000003726 plant lectin Substances 0.000 description 1
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- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- 239000008223 sterile water Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
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- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
- Zoology (AREA)
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- Wood Science & Technology (AREA)
- Polymers & Plastics (AREA)
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- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Molecular Biology (AREA)
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Abstract
The present invention provides a kind of mixing probiotics for bean pulp fermentation, and the mixing probiotics is mixed by the strain of following parts by weight：3-5 parts of lactobacillus reuteri, 3-5 parts of lactobacillus plantarum, 1-3 parts of bacillus subtilis, 1-3 parts of aspergillus oryzae.The present invention also provides the methods of above-mentioned mixing probiotics fermention dregs of beans.The present invention also provides the fermented products that above-mentioned fermentation process ferments.The present invention for the mixing probiotics of bean pulp fermentation and its fermentation process, can a variety of anti-nutritional factors in fully degraded dregs of beans, be added into the digestibility that animal can be improved in feed to feed；It is also rich in prebiotic thalline and beneficial metabolic product, part antibiotic can be played the role of by being added in feed, while help to alleviate the oxidative stress of swinery, improved animal products quality, improved breeding performonce fo animals and immunocompetence.
Description
Technical field
The present invention relates to feed addictives more particularly to a kind of for the mixing probiotics of bean pulp fermentation and its fermentation side
Method.
Background technology
Dregs of beans becomes widest plant egg because it has more balanced amino acid composition and good nutritive peculiarity
The first choice of protein resource in white matter raw material and feed.But due to containing in dregs of beans, there are many anti-nutritional factors, such as tryptose
Enzyme inhibitor, phytolectin, oligosaccharide and antigen protein etc. limit the use of dregs of beans.
Simultaneously as the improvement of people's living standards, requirement of the people to animal products quality is also higher and higher, reduce anti-
Raw the element even trend for being added to livestock-raising future development of antibiotic-free.
The study found that microorganism can be used for fermented bean dregs, to obtain the fermented product with nutritive value.But it is existing
Zymotechnique used by strain, technique it is different, be primarily present problems with：The nutrition in dregs of beans cannot be made full use of
Ingredient, fermented product have a single function.
Therefore, a kind of microorganism formulation can make full use of the nutritional ingredient in dregs of beans is researched and developed, can be raised making an addition to
Feed addictive with more wide variety of functions when in material has a vast market foreground.
Invention content
The technical problem to be solved by the present invention is to：In view of the drawbacks of the prior art, it provides a kind of for bean pulp fermentation
Mix probiotics and its fermentation process, can a variety of anti-nutritional factors in fully degraded dregs of beans, being added into feed can improve
Digestibility of the animal to feed；It is also rich in prebiotic thalline and beneficial metabolic product, part can be played by being added in feed
The effect of antibiotic, at the same contribute to alleviate swinery oxidative stress, improve animal products quality, improve breeding performonce fo animals and
Immunocompetence.
The present invention uses following technical scheme to solve above-mentioned technical problem：
It is an object of the present invention to provide a kind of mixing probiotics for bean pulp fermentation, the mixing probiotics by with
The strain of lower parts by weight mixes：
In order to optimize above-mentioned technical solution, the technical measures that the present invention takes further include：
Further, the mixing probiotics is mixed by the strain of following parts by weight：
It is a further object to provide the methods of above-mentioned mixing probiotics fermention dregs of beans, by dregs of beans and corn, rice
After the chaff dregs of rice, skimmed milk power, whey powder and glucose mixing, it is 1.5 that a certain amount of water, which is added, and adjusts material-water ratio:1 (material：Water),
It is used as solid-state fermentation culture medium after mixing, sterilizing, mixing probiotics is seeded in solid-state hair according to the weight ratio of 4%-10%
In ferment culture medium, fermentation time 45-60h.
Further, the weight percent of each component is in the solid-state fermentation culture medium：Dregs of beans 15%-30%, corn
20%-60%, rice bran meal 5%-15%, skimmed milk power 5%-20%, whey powder 2%-15%, glucose 1%-10%.
Further, the mixing probiotics is seeded in solid-state fermentation culture medium according to 4%~10% weight ratio.
Further, the solid-state fermentation culture medium after fermentation is dry, pulverize and sieve, sieve is 40-60 mesh, must both be fermented
Product.Preferably cross 40 mesh sieve.
It is a further object to provide the fermented products that above-mentioned fermentation process ferments.
Lactobacillus reuteri can be colonized in enteron aisle, inhibited pathogenic entero becteria breeding, improved immune function, reduce animal oxygen
Change stress, reduce animal disease, adjust intestinal flora distribution, and lactobacillus reuteri can generate a kind of the wide of non-protein class
Compose antibacterial substance Luo Yishi bacteriocins；Lactobacillus plantarum not only acts as the effect of leavening, moreover it is possible to generate wind during the fermentation
Taste substance improves the palatability of feed, while lactobacillus plantarum also has the function of alleviating oxidative stress, so as to promote pig
The health and improvement animal products quality of group；Bacillus subtilis secretes multiple protein enzyme in growth course can degrade dregs of beans
In macro-molecular protein include antigen protein, make it become to be conducive to the peptides digested and assimilated and amino acid；Aspergillus oryzae fermented
Generation enzyme related with carbon hydrate can be metabolized in journey, cellulose, hemicellulose, starch and pectin etc. in raw material of degrading
Macromolecular substances, so as to cause thick protein, the relative increase of total energy and fat.Strain combination is matched during the present invention uses above-mentioned 4
5, it cooperates between each strain, cooperative fermentation, using metabolic characteristic possessed by various probiotics, for fermenting with dregs of beans,
The anti-nutritional factors that can not only be degraded in dregs of beans, additionally it is possible to fully decompose high molecular weight protein in dregs of beans, cellulose, hemicellulose
The macromolecular substances such as element, starch and pectin assign dregs of beans new trophic function, obtain it is full of nutrition and with bacteria resistance function,
The fermented product of good mouthfeel.
The present invention is had the following technical effect that compared with prior art using above-mentioned technical proposal：
The raw materials such as mixing probiotic combinations fermented bean dregs of the present invention, can not only a variety of anti-nutrition in fully degraded dregs of beans
The factor is added into the digestibility that animal can be improved in feed to feed；Also make product rich in prebiotic thalline and beneficial to generation
Thank to product, fermented product, which is added in feed, can play the role of part antibiotic, while help to alleviate the oxidation of swinery
Stress, improve animal products quality, improve breeding performonce fo animals and immunocompetence, the comprehensive utilization for improving dregs of beans has weight
The meaning wanted.
Description of the drawings
Fig. 1 is the fungistatic effect schematic diagram that probiotics fermention product is mixed in embodiment 2 to k88.
Specific implementation mode
Technical scheme of the present invention is described in further detail with reference to embodiment.Following embodiment is only used for more
Add and clearly demonstrate technical scheme of the present invention, and not intended to limit the protection scope of the present invention.
Embodiment 1
In the present embodiment, lactobacillus reuteri, lactobacillus plantarum, bacillus subtilis and aspergillus oryzae are according to 3:3：2:2
Weight ratio is mixed to get mixing probiotics, and solid-state fermentation culture medium, material-water ratio 1.5 are accessed according to 8% inoculum concentration:1
(material：Water), fermentation time 48h is dry by the solid-state fermentation culture medium after fermentation after fermentation, crushed 40 mesh sieve, obtains
Fermented product.
Wherein, the weight percent of each component is in solid-state fermentation culture medium：Dregs of beans 15%-30%, corn 20%-
60%, rice bran meal 5%-15%, skimmed milk power 5%-20%, whey powder 2%-15%, glucose 1%-10%.
Embodiment 2
The present embodiment is verification embodiment.
The present embodiment verifies the suppression of the mixing probiotics fermention product of the present invention using E.coli K88 as experimental subjects
Bacterium ability.
Weigh made from 1g fermentation process according to the invention that fermented product is in sterilizing test tubes, by 1:2 (m/v) ratios
Sterile water, mixing is added, 3000r/min centrifuges 10min, and supernatant is taken to carry out bacteriostatic experiment to E.coli K88.Bacteriostatic experiment
Each tablet adds 100 μ L of pathogenic bacteria to carry out coated plate, is loaded using Oxford cup, 200 μ L of product are loaded per hole, each pathogenic bacteria is cooked
2 parallel, spreads 1h in 4 DEG C of refrigerators after sample addition, is subsequently placed in 37 DEG C of incubators and cultivates 8h.
Observation is taken out, as a result as shown in Fig. 1.Wherein, 2 parallel group inhibition zones on top be experimental group, 2 of lower part
Parallel group inhibition zone is blank control group.
By experimental result, it can be seen that, fermented product of the invention has apparent good fungistatic effect.Therefore, this is sent out
Bright fermented product, which makes an addition in feed, to inhibit animal enterpathogen to breed, and improve Immune Function In Animals.
Embodiment 3
The present embodiment is verification embodiment.
The variation of dregs of beans nutritional ingredient before and after the mixing probiotics fermention dregs of beans of the present embodiment verification present invention.
The experimental results showed that under same dry matter basis, it is thick in the dregs of beans after the mixing probiotics fermention of the present invention
The more non-fermented bean dregs of albumen, which are compared, improves 5.21%, and reaches 5.81 × 108cfu/g beneficial to bacterium number, and lactic acid content reaches
6.14%；Antigen protein and bad oligosaccharides are respectively by SDS-PAGE electrophoretic analysis and TLC plate analysis, compared to fermentation after fermentation
Before, antigen protein and the degradation of bad oligosaccharides almost all.
The above results show the anti-nutritional factors that mixing probiotics of the invention can not only degrade in dregs of beans, additionally it is possible to
The macromolecular substances such as high molecular weight protein, cellulose, hemicellulose, starch and the pectin in dregs of beans are fully decomposed, it is new to assign dregs of beans
Trophic function；Rich in prebiotic thalline and beneficial metabolic product, inhibit pathogenic entero becteria breeding, being added in feed can play
The effect of part antibiotic.
Embodiment 4
The present embodiment is verification embodiment.
Free radical scavenging activity (DPPH) be it is a kind of it is artificial synthesized with Single Electron centered on nitrogen
Organic free radical with good stability energy；The present embodiment verifies this hair by the measurement to DPPH Scavenging abilities
The oxidation resistance of bright mixing probiotics fermention product.
The measurement of scavenging ability of DPPH free radical is defined as 1 active unit (U) to 50% clearance rate of DPPH free radicals,
DPPH free radical scavenging abilities indicate (U/g with the active unit contained in the fermented product extracting solution sample of every gram of dry weight
DW)。
Experimental method：After fermented sample crushed 40 mesh sieve, with 50% ethyl alcohol with 1:20 solid-liquid ratios, under the conditions of 55 DEG C
6h is impregnated, 3000r/min centrifuges 10min and obtains supernatant, its ability for removing DPPH free radicals is measured with supernatant.
Experimental result：The fermented product for obtaining the present invention after measured removes the ability of DPPH free radicals as 2124U/g DW.
This example demonstrates that the fermented product of the present invention has stronger oxidation resistance, the fermented product of the present invention is added
It is added on the oxidative stress that can aid in feed and alleviate swinery, improves animal products quality, improves breeding performonce fo animals.
Embodiment 5
The present embodiment is verification embodiment.
Following experimental group and control group is arranged in the present embodiment, verifies work of the fermented product to growth of animal performance of the present invention
With.
Experimental group uses the fermented product that the embodiment of the present invention 1 obtains；
Control group uses a kind of public for the composite flora of bean pulp fermentation and its application institute according to patent CN104762234A
Fermented product prepared by the embodiment 4 for the method opened；
Experimental method：To be tested in Jiangsu source one pork pig farm of credit, experimental period is 2 months；Choose health, body
The approximate pig of weight 100, is randomly divided into 2 groups of experimental group and control group, the fermented product for respectively obtaining experimental group and control group
It is made an addition in pannage according to 10% weight ratio, the incidence of pig during statistical experiment, and the front and back pig of experiment
Weight, the experimental results are shown inthe following table (weight of pig is averaged)：
By upper table result it is found that relative to control group, fermented product of the invention can preferably reduce the morbidity of pig
Rate adjusts the immunocompetence of pig, promotes the growth of pig.
By above-described embodiment it is found that the raw materials such as mixing probiotic combinations fermented bean dregs of the present invention, can not only fully drop
A variety of anti-nutritional factors in dregs of beans are solved, the digestibility that can improve animal in feed to feed is added into；Also make product rich
Containing prebiotic thalline and beneficial metabolic product, fermented product, which is added in feed, can play the role of part antibiotic, have simultaneously
The oxidative stress for helping alleviate swinery, improves animal products quality, improves breeding performonce fo animals and immunocompetence, for improving beans
The comprehensive utilization of the dregs of rice has great importance.
Specific embodiments of the present invention are described in detail above, but it is only used as example, the present invention is not intended to limit
In particular embodiments described above.To those skilled in the art, it is any to the practicality carry out equivalent modifications and replace
In generation, is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by impartial conversion and repair
Change, all should be contained within the scope of the invention.
Claims (7)
1. a kind of mixing probiotics for bean pulp fermentation, which is characterized in that the mixing probiotics is by following parts by weight
Strain mixes：
2. mixing probiotics according to claim 1, which is characterized in that the mixing probiotics is by following parts by weight
Strain mixes：
3. the method for mixing probiotics fermention dregs of beans according to claim 1, which is characterized in that by dregs of beans and corn, rice
After the chaff dregs of rice, skimmed milk power, whey powder and glucose mixing, it is 1.5 that a certain amount of water, which is added, and adjusts material-water ratio:1 (material：Water),
It is used as solid-state fermentation culture medium after mixing, sterilizing, mixing probiotics is seeded in solid-state hair according to the weight ratio of 4%-10%
In ferment culture medium, fermentation time 45-60h.
4. according to the method described in claim 3, it is characterized in that, in the solid-state fermentation culture medium each component weight percent
Than for：Dregs of beans 15%-30%, corn 20%-60%, rice bran meal 5%-15%, skimmed milk power 5%-20%, whey powder 2%-
15%, glucose 1%-10%.
5. according to the method described in claim 3, it is characterized in that, described mix weight ratio of the probiotics according to 4%-10%
It is seeded in solid-state fermentation culture medium.
6. according to the method described in claim 3, it is characterized in that, the solid-state fermentation culture medium after fermentation is dry, crushed
Sieve, sieve are 40-60 mesh to get fermented product.
7. the fermented product that the method according to any one of claim 3-6 is fermented.
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| CN113528380A (en) * | 2021-06-28 | 2021-10-22 | 国家粮食和物资储备局科学研究院 | Multi-strain composite brown rice solid-state fermentation microbial inoculum, and preparation method and application thereof |
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