CN107950753A - A kind of method of aerobic-anaerobic coupled fermentation production biological feedstuff - Google Patents
A kind of method of aerobic-anaerobic coupled fermentation production biological feedstuff Download PDFInfo
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- CN107950753A CN107950753A CN201711461225.7A CN201711461225A CN107950753A CN 107950753 A CN107950753 A CN 107950753A CN 201711461225 A CN201711461225 A CN 201711461225A CN 107950753 A CN107950753 A CN 107950753A
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- fermentation
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- biological feedstuff
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- 238000000855 fermentation Methods 0.000 title claims abstract description 124
- 230000004151 fermentation Effects 0.000 title claims abstract description 119
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 81
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 26
- 238000009423 ventilation Methods 0.000 claims abstract description 18
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 16
- 241000186660 Lactobacillus Species 0.000 claims abstract description 8
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 33
- 244000063299 Bacillus subtilis Species 0.000 claims description 19
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 19
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 19
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 19
- 241000186604 Lactobacillus reuteri Species 0.000 claims description 17
- 229940001882 lactobacillus reuteri Drugs 0.000 claims description 17
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 13
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 13
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 13
- 238000001816 cooling Methods 0.000 claims description 13
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 13
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- 239000004365 Protease Substances 0.000 claims description 11
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
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- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 4
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- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
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- 238000007789 sealing Methods 0.000 claims description 3
- 101800000263 Acidic protein Proteins 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 238000011049 filling Methods 0.000 claims description 2
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- 230000005611 electricity Effects 0.000 claims 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 26
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- 239000004310 lactic acid Substances 0.000 abstract description 13
- 235000014655 lactic acid Nutrition 0.000 abstract description 13
- 230000012010 growth Effects 0.000 abstract description 11
- 244000005700 microbiome Species 0.000 abstract description 9
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- 239000007787 solid Substances 0.000 abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 3
- 235000021050 feed intake Nutrition 0.000 abstract description 3
- 239000003205 fragrance Substances 0.000 abstract description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 3
- 230000036541 health Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
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- 210000000936 intestine Anatomy 0.000 abstract description 2
- 235000019629 palatability Nutrition 0.000 abstract description 2
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- 239000000243 solution Substances 0.000 description 11
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- 239000000047 product Substances 0.000 description 5
- 239000003674 animal food additive Substances 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
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- 102000004169 proteins and genes Human genes 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
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- 238000005303 weighing Methods 0.000 description 2
- GSDSWSVVBLHKDQ-UHFFFAOYSA-N 9-fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid Chemical compound FC1=CC(C(C(C(O)=O)=C2)=O)=C3N2C(C)COC3=C1N1CCN(C)CC1 GSDSWSVVBLHKDQ-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 108010078777 Colistin Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- BDKZHNJTLHOSDW-UHFFFAOYSA-N [Na].CC(O)=O Chemical compound [Na].CC(O)=O BDKZHNJTLHOSDW-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
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- 239000000686 essence Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229960002422 lomefloxacin Drugs 0.000 description 1
- ZEKZLJVOYLTDKK-UHFFFAOYSA-N lomefloxacin Chemical compound FC1=C2N(CC)C=C(C(O)=O)C(=O)C2=CC(F)=C1N1CCNC(C)C1 ZEKZLJVOYLTDKK-UHFFFAOYSA-N 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- BZDIAFGKSAYYFC-UHFFFAOYSA-N manganese;hydrate Chemical compound O.[Mn] BZDIAFGKSAYYFC-UHFFFAOYSA-N 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- JORAUNFTUVJTNG-BSTBCYLQSA-N n-[(2s)-4-amino-1-[[(2s,3r)-1-[[(2s)-4-amino-1-oxo-1-[[(3s,6s,9s,12s,15r,18s,21s)-6,9,18-tris(2-aminoethyl)-3-[(1r)-1-hydroxyethyl]-12,15-bis(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-h Chemical compound CC(C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@H]([C@@H](C)O)CN[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CCN)NC1=O.CCC(C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@H]([C@@H](C)O)CN[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CCN)NC1=O JORAUNFTUVJTNG-BSTBCYLQSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229960001699 ofloxacin Drugs 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- FHFYDNQZQSQIAI-UHFFFAOYSA-N pefloxacin Chemical compound C1=C2N(CC)C=C(C(O)=O)C(=O)C2=CC(F)=C1N1CCN(C)CC1 FHFYDNQZQSQIAI-UHFFFAOYSA-N 0.000 description 1
- 229960004236 pefloxacin Drugs 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- XDJYMJULXQKGMM-UHFFFAOYSA-N polymyxin E1 Natural products CCC(C)CCCCC(=O)NC(CCN)C(=O)NC(C(C)O)C(=O)NC(CCN)C(=O)NC1CCNC(=O)C(C(C)O)NC(=O)C(CCN)NC(=O)C(CCN)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)C(CCN)NC1=O XDJYMJULXQKGMM-UHFFFAOYSA-N 0.000 description 1
- KNIWPHSUTGNZST-UHFFFAOYSA-N polymyxin E2 Natural products CC(C)CCCCC(=O)NC(CCN)C(=O)NC(C(C)O)C(=O)NC(CCN)C(=O)NC1CCNC(=O)C(C(C)O)NC(=O)C(CCN)NC(=O)C(CCN)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)C(CCN)NC1=O KNIWPHSUTGNZST-UHFFFAOYSA-N 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 150000007660 quinolones Chemical class 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/20—Control of temperature characterised by the use of electric means with sensing elements having variation of electric or magnetic properties with change of temperature
- G05D23/24—Control of temperature characterised by the use of electric means with sensing elements having variation of electric or magnetic properties with change of temperature the sensing element having a resistance varying with temperature, e.g. a thermistor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/173—Reuteri
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P80/00—Climate change mitigation technologies for sector-wide applications
- Y02P80/10—Efficient use of energy, e.g. using compressed air or pressurized fluid as energy carrier
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Automation & Control Theory (AREA)
- Fodder In General (AREA)
Abstract
The present invention discloses a kind of method of aerobic-anaerobic coupled fermentation production biological feedstuff.The present invention uses the fermenting microbe of bacillus, lactobacillus and saccharomycete Mixed Microbes as biological feedstuff, biological feedstuff is produced by using aerobic-anaerobic coupling solid zymotechnique, it is intermittent toward being passed through sterile constant-temperature air in deep layer material during the fermentation, be conducive to growth of aerobic microorganisms in lower floor's material to breed, be also beneficial to improve product quality stability；The piling height of the bed of material, the production capacity of lifting means can be improved in ventilation and heat situation.In addition, containing the probiotics that can largely improve animal intestinal tract health after biological feedstuff is fermented, the micro-ecological environment of intestines and stomach can be improved after feeding animals, improve the immunocompetence of body.In addition, biological feedstuff is rich in lactic acid and has strong alcohol fragrance, feed palatability can be significantly improved, is conducive to improve animal feed intake, promotes fast growth of animal, improve cultivation industry production capacity.
Description
Technical field
The present invention relates to Feed Manufacturing field, more particularly to a kind of side of aerobic-anaerobic coupled fermentation production biological feedstuff
Method.
Background technology
In cultivation industry since feeding antibiotic is largely using caused medicament residue, drug resistance and the problems such as food security
Increasingly serious, the developed country such as America and Europe has started to disable feeding antibiotic comprehensively, and the Chinese government also has begun to limit or disable
Part feeding antibiotic, as the end of the year 2015 disabled Lomefloxacin, Pefloxacin, Ofloxacin, promise fluorine sand in food animal
Appearance as defined in feed addictive purposes of 4 kinds of quinolones veterinary drugs of star and in November, 2016 stopping sulfate colistin etc.,
Limitation is reduced in animal or the government action of stopping antibiotic usage is significantly accelerated, and it will be general trend of events institute to disable feeding antibiotic comprehensively
Become.In " rear antibiotic " epoch, biological feedstuff application is also increasingly taken seriously, and biological feedstuff is a large amount of in cultivation industry in recent years
Using especially prevalent.Biological feedstuff is using feedstuff or agricultural and sideline product as primary raw material, passes through inoculation yeast bacterium, gemma bar
The beneficial microbe such as bacterium and lactic acid bacteria, under artificial controllable condition, utilizes the microorganisms such as lactic acid bacteria, saccharomycete and bacillus
The growth metabolism activity of itself, the anti-nutritional factors in raw material and macromolecular substances are decomposed or conversion, metabolism generate organic acid
With the small-molecule substance such as soluble small peptide, form that nutritive value is high, palatability is good, absorption easy to digest and the biology rich in probiotics
Feed.
Biological feedstuff fermentation generally use multi-cultur es mixed solid fermentation mode produces at present, is usually used in fermenting organism feed
Strain have the kinds such as saccharomycete, bacillus and lactic acid bacteria.Common bacillus such as bacillus subtilis and lichens gemma bar
Bacterium etc. can secrete a large amount of protease, and the high molecular weight protein that can be degraded including antigen protein, it is special to be conducive to raising animal
It is young animal to absorbing proteins；Yeast Growth metabolism produces a large amount of mycoprotein, fat, polysaccharide and B family vitamins, and
The beneficiating ingredients such as enzyme, coenzyme, ribonucleic acid, sterol and some unknown growth factor, can improve the nutritive value of feed, in addition
Feed can also produce strong alcohol fragrance after yeast ferments, and be conducive to improve animal feed intake；Lactic acid bacteria be widely present in poultry,
In fowl enteron aisle, lactic acid bacteria can adjust body gastrointestinal tract normal flora, keep microecological balance, improve food digestion rate, moreover it is possible to press down
Harmful microorganism growth and breeding in enteron aisle processed, plays a significant role in terms of animal body health is safeguarded.In above-mentioned common three classes
In major microorganisms, bacillus belongs to aerobic microbiological, and saccharomycete then belongs to facultative aerobic microbiological, and lactic acid bacteria belongs to more
Amphimicrobe or strictly anaerobic microorganism.When using bacillus, saccharomycete and lactic acid bacteria multiple bacteria compound fermentation,
It is particularly important that each strain raised growth is bred is coordinated there are greatest differences to oxygen demand due to each strain.
In current feed fermentation production method, some are fermented using a step anaerobic fermentation mode, that is, are being inoculated with
Anaerobic fermentation is directly carried out after lactobacillus, bacillus and saccharomycete；Other are the two-step fermentations using first aerobic rear anaerobism
Mode is fermented, i.e., inoculates anaerobic bacteria progress anaerobic fermentation after being first inoculated with aerobic bacteria shallow tray fermentation.Sent out for a step anaerobism
For ferment, such a mode is unable to amount reproduction in the presence of the aerobic microbiological such as bacillus and saccharomycete caused by for hypoxgia,
The problems such as so as to can not effectively play the physiological function of bacillus and saccharomycete；For two-step fermentation, such a mode is deposited
Grown in technological process, mass produce the problems such as manipulation strength is big, and product quality is difficult to control.In biological feedstuff production process
In, how regulating and controlling bacillus, saccharomycete and lactic acid bacteria etc., Bu Tong aerobic to require the breeding of microorganism raised growth be production Gao Pin
The key of matter biological feedstuff.
The content of the invention
The shortcomings that in order to overcome the prior art and deficiency, primary and foremost purpose of the invention are that providing one kind is used for biological feedstuff
The ventilation temperature-controlling system of aerobic-anaerobic coupled fermentation.
Another object of the present invention is to provide a kind of method of aerobic-anaerobic coupled fermentation production biological feedstuff.
The present invention uses bacillus, lactobacillus and saccharomycete mixed solid fermentation technique, by intermittent toward deep layer thing
Sterile constant-temperature air is passed through in material, the required oxygen of limit is provided with for aerobic microbiological, is conducive to aerobic micro- life in lower floor's material
Thing growth and breeding.Meanwhile monitor fermentation materials temperature in real time during the fermentation, once material temperature exceedes microorganism suitable growth temperature
Degree, can also be cooled down for fermentation materials by being passed through Tempeerature-constant air, improved product quality stability.
It is still another object of the present invention to provide a kind of biological feedstuff.
The purpose of the present invention is achieved through the following technical solutions：
A kind of ventilation temperature-controlling system for biological feedstuff aerobic-anaerobic coupled fermentation, including blowing plant, rotor
Flowmeter, air filter, air heater, fermentation temperature regulating device, venting in timing manner control device, air sparger, fermentation are held
Device；Filling biological feedstuff in round；
The blowing plant, spinner flowmeter, air filter, air heater, air sparger lead to successively
Piping is connected；
The fermentation temperature regulating device includes temperature sensor, temp controlled meter and the first solenoid valve, the temperature sensor
It is connected with temp controlled meter signal, temp controlled meter is connected with the first solenoid signal；Temperature sensor is used to detect biological feedstuff fermentation temperature
Degree, and temp controlled meter is transmitted a signal to, whether temp controlled meter controls the cooling of the first electromagnetic valve switch.
The venting in timing manner control device includes time controller and second solenoid valve, the time controller and
Two solenoid signals connect；Ventilate in being switched by setting time controller timing controlled second solenoid valve toward biological feedstuff.
The air heater is connected by the first pipeline with first solenoid valve one end, and the first solenoid valve other end passes through
Second pipe is connected with air sparger；
Described second solenoid valve one end is connected with the first pipeline, and the other end is connected with second pipe.
Preferably, the blowing plant includes air compressor machine, air accumulator and cooling driers, the air compressor machine, gas storage
Tank passes sequentially through pressure pipeline with cooling driers and is connected.
One end of the spinner flowmeter is connected with cooling driers, the other end and the air filter phase of spinner flowmeter
Even.
The air filter is pipelined filter, degerming filter core built in air filter.
The air filter is used to remove the microorganism in air.
The air heater is the heating unit with temperature control modules, is provided with the gas outlet of air heater
Temperature transducer, controls heater to heat air, in order to provide the compressed air of constant temperature by setting leaving air temp.
Preferably, the probe of the temperature sensor is located at biological feedstuff lower middle position.
The air sparger is the round tube of lower end sealing, and the upper port of air sparger is connected with second pipe,
The lower part of the pipe side wall of air sparger is provided with some air holes；Air sparger has multiple.
The air sparger is inserted perpendicularly into biological feedstuff, and air hole is located in biological feedstuff, and uppermost
The distance of stomata to biological feedstuff upper surface is setting value, general to be no less than 1/3rd of thickness of feed layer, to prevent " air is short
Road ".
The round is the large-scale shedding motion of sealed bottom, and round can use fermentation vat, fermentation tank, hair
Ferment bucket or fermentation bag, round bottom need to seal and be unable to gas leakage.
The present invention is provided with a second solenoid valve in parallel near the first solenoid valve, this second solenoid valve is controlled with the time
Device processed is connected, can be for a long time or intermittent toward being passed through constant temperature compressed air in biological feedstuff by setting time controller.
A kind of method of aerobic-anaerobic coupled fermentation production biological feedstuff, includes the following steps：
(1) pretreatment of raw material
Select without mouldy, free from extraneous odour and without rotten dregs of beans, skin of beancurd be used as fermentation raw material, dregs of beans dosage be 90%~
100%, skin of beancurd dosage is 0~10%, and raw material is uniformly mixed and is used as fermentation base-material through pulverizing and sieving by raw material according to formula；
Preferably, the sieving was 10 mesh sieves.
(2) prepare biological feedstuff fermentation and use mixed bacteria seed liquor
By the saccharomycete activated (Saccharomyces cerevisiae), bacillus subtilis (Bacillus
Subtilis), lactobacillus plantarum (Lactobacillus plantarum) and lactobacillus reuteri (Lactobacillus
Reuteri) additive amount is poured into nutrient solution strain on demand, is thoroughly mixed uniformly, nutrient solution water temperature is controlled 30 ± 1
DEG C, this mixed bacteria liquid is bean pulp fermentation seed liquor；Again toward addition acid protease, neutral proteinase in bean pulp fermentation seed liquor
And alkali protease, each protease additive amount is on the basis of the base-material that ferments, i.e.,：Alkali protease additive amount 25~100U/g, it is excellent
Select 50U/g；Neutral proteinase 25~100U/g of additive amount, preferably 50U/g；Acidic protein enzyme amount 10~50U/g of additive amount, preferably
25U/g, it is that biological feedstuff fermentation Mixed Microbes are a variety of after mixing with bean pulp fermentation seed liquor in right amount to weigh each protease
Sub- liquid；
Saccharomycete and bacillus content are in the bean pulp fermentation seed liquor：Saccharomycete 1 × 107~3 × 107cfu/
ML, preferred concentration are 2 × 107cfu/mL；Bacillus subtilis 1 × 107~5 × 107Cfu/mL, preferred concentration for 3 ×
107cfu/mL；Lactobacillus plantarum 1 × 107~3 × 107Cfu/mL, preferred concentration are 2 × 107cfu/mL；Lactobacillus reuteri 1
×107~3 × 107Cfu/mL, preferred concentration are 2 × 107cfu/mL。
The mass percent meter of component contained by the nutrient solution is as follows：Glucose or sucrose 2%, K2HPO40.3% He
KH2PO40.2%.
Preferably, the saccharomycete is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can yeast for animal feeds bacterium,
Including Saccharomyces cerevisiae NKY1 bacterial strains.Wherein, Saccharomyces cerevisiae NKY1 are special
It is public in profit " 201410234358.0, a kind of feeding thermostable microcapsule yeast probiotics preparation and preparation method and application "
Open.
Preferably, the bacillus subtilis is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding
Bacillus subtilis.
Preferably, the lactobacillus plantarum is promulgated for the Ministry of Agriculture《Catalogue of feed additive varieties》In can be feeding plant
Thing bacillus.
Preferably, the lactobacillus reuteri Ministry of Agriculture promulgates《Catalogue of feed additive varieties》In can be feeding sieve
Yi Shi lactobacillus；Including Lactobacillus reuteri LR1 bacterial strains.Wherein, Lactobacillus reuteri LR1 exist
Disclosed in patent " 201510751130.3, one plants of L. reuteri strains with prebiotic characteristics and its application ".
(3) base-material ferments
The biological feedstuff fermentation mixed bacteria described in step (2) is uniformly sprayed into the fermentation base-material described in step (1)
Seed liquor, control base-material moisture is between 35%~45%, and preferably moisture is 40%, and base-material is fully mixed with mixed bacteria seed liquor
Close the round (hair that the fermentation base-material being inoculated with uniformly is encased in above-mentioned aerobic-anaerobic coupled fermentation ventilation temperature-controlling system
Ferment bucket or fermentation vat) in, base-material ulking thickness is inserted into air sparger pipe, at the same time to be advisable no more than 100cm in fermentation materials
Thermometric temperature sensor is inserted into, it is 32~37 DEG C to control fermentation temperature, and ferment 36h~48h under controlled temperature conditions of divulging information, and then detests
24~48h of aerobe fermentation, obtains biological feedstuff.
Preferably, the air outlet temperature of air heater is arranged to 37 DEG C, passage time controller control air pipe line
Upper second solenoid valve switch intermittence is passed through Tempeerature-constant air into material, earlier fermentation (before material temperature does not rise) set at interval of
Every is set toward 5~15 seconds (preferably 10 seconds) Tempeerature-constant airs are passed through in material, in the fermentation materials material temperature rise middle and later periods within 10 minutes
5~15 seconds (preferably 10 seconds) air were passed through every 5 minutes；Fermentation materials temperature is controlled between 32~37 DEG C, once fermentation materials
More than 37 DEG C, by cooling down to material toward being passed through air in material.
Preferably, it is as follows to prepare situation for the activation of the saccharomycete described in step (2)：
The active dry yeast bacterium for weighing a certain amount of known viable count is added in yeast activated liquid in 5% (m/v) ratio and (contains
0.9%NaCl and 2% glucose), 30 DEG C of concussion activation 30min.
Preferably, it is as follows to prepare situation for the activation of the bacillus subtilis described in step (2)：
The bacillus subtilis powder for weighing a certain amount of known viable count is added in actication of culture liquid in 2% (m/v) ratio
(containing 0.9%NaCl and 2% glucose), 37 DEG C of concussion activation 40min.
Preferably, it is as follows to prepare situation for the activation of the lactobacillus plantarum described in step (2)：
Lactobacillus plantarum is seeded to progress level-one culture in MRS fluid nutrient mediums, after 37 DEG C are cultivated 24h, is connect according to 5%
Kind amount carries out secondary amplification culture.
Preferably, it is as follows to prepare situation for the activation of the lactobacillus reuteri described in step (2)：
Lactobacillus reuteri is seeded to progress level-one culture in MRS fluid nutrient mediums, after 37 DEG C are cultivated 24h, according to 5%
Inoculum concentration carries out secondary amplification culture.
The MRS fluid nutrient mediums are：Casein peptone 10g/L, beef extract powder 10g/L, glucose 20g/L, yeast carry
Take thing 5g/L, dipotassium hydrogen phosphate 2g/L, sodium citrate 2g/L, epsom salt 0.58g/L, four water manganese sulfate 0.25g/L, acetic acid
Sodium 5g/L, Tween-80 1mL/L, agar 15g/L；Adjust pH5.5.
A kind of biological feedstuff, ferments to obtain by the above method.
The present invention is had the following advantages relative to the prior art and effect：
The present invention uses the fermenting microbe of bacillus, lactobacillus and saccharomycete Mixed Microbes as biological feedstuff, by adopting
Biological feedstuff is produced with aerobic-anaerobic coupling solid zymotechnique, it is intermittent sterile toward being passed through in deep layer material during the fermentation
Tempeerature-constant air, the required oxygen of limit is provided with for aerobic microbiological, is conducive to growth of aerobic microorganisms in lower floor's material and is bred.Together
When, during the fermentation by the fermentation temperature of draft type control material in OK range, be conducive to improve product quality steady
It is qualitative；The piling height of the bed of material, the production capacity of lifting means can be improved in ventilation and heat situation.In addition, biological feedstuff passes through
The probiotics of animal intestinal tract health can largely be improved by containing after fermentation, can improve the micro-ecological environment of intestines and stomach after feeding animals,
Improve the immunocompetence of body.In addition, biological feedstuff is rich in lactic acid and has strong alcohol fragrance, it is agreeable to the taste feed can be significantly improved
Property, be conducive to improve animal feed intake, promote fast growth of animal, cultivation industry production capacity can be improved.
Brief description of the drawings
Fig. 1 is a kind of aerobic-anaerobic coupled fermentation ventilation control for biological feedstuff fermentation that the embodiment of the present invention is provided
Warm system flow schematic diagram.
Fig. 2 is air sparger in the embodiment of the present invention.
Wherein, 1, air compressor machine；2nd, air accumulator；3rd, cooling driers；4th, spinner flowmeter；5th, air filter；6th, air heats
Device；7th, temp controlled meter；8th, the first solenoid valve；9th, PT100 temperature sensors；10th, time controller；11st, air sparger；12nd, send out
Ferment container；13rd, round tube；14th, air hole；15th, second solenoid valve；16th, the first pipeline；17th, second pipe.
Embodiment
With reference to embodiment and attached drawing, the present invention is described in further detail, but embodiments of the present invention are unlimited
In this.
Embodiment 1
As shown in Figure 1 and Figure 2, a kind of ventilation control for biological feedstuff aerobic-anaerobic coupled fermentation is present embodiments provided
Warm system, including：Blowing plant (air compressor machine 1, air accumulator 2 and cooling driers 3), spinner flowmeter 4, air filter 5, sky
Hot-air heater 6, fermentation temperature regulating device (temp controlled meter 7, the first solenoid valve 8 and PT100 temperature sensors 9), venting in timing manner control dress
Put (time controller 10 and second solenoid valve 15), air sparger 11 and round 12.The air compressor machine 1, air accumulator 2
It is connected between cooling driers 3 using pressure pipeline.One end of the spinner flowmeter 4 is connected with cooling driers 3, spinner flowmeter
4 other end is connected with air filter 5.The air filter 5 is connected by pipeline with 6 air inlet of air heater；
The gas outlet of the air heater 6 is connected by the first pipeline 16 with 8 one end of the first solenoid valve, and the first solenoid valve 8 is another
End is connected by second pipe 17 with air sparger 11；Described 15 one end of second solenoid valve is connected with the first pipeline 16, separately
One end is connected with second pipe 17.Temp controlled meter 7, the first solenoid valve 8 and the temperature sensor 9 is connected with each other composition biology and raises
Expect fermentation temperature control device；The time controller 10 is connected to form venting in timing manner control device with second solenoid valve 15.
PT100 temperature sensors 9 are used to detect the temperature inside biological feedstuff, and signal transmission will be detected to temp controlled meter
7, if detecting, temperature is higher than given threshold, and temp controlled meter output signal controls the first solenoid valve 8 to lead in opening toward round
Enter air, ensure that biological feedstuff temperature is in and be adapted to fermentation temperature.
Air heater 6 is used to heat cold dry air to steady temperature,
It is intermittent toward being passed through Tempeerature-constant air, root in round that time controller 10 is used to controlling second solenoid valve 15 to open
Aerobic strain situation is inoculated with according to biological feedstuff, one is passed through toward biological feedstuff intermittent by 10 timing of setting time controller
Fix time air.Time controller 10 can the timing when fermenting beginning.
The air sparger 11 is the round tube 13 of lower end sealing, and port is connected with second pipe 17 thereon, air
The lower part of the pipe side wall of distributor 11 is provided with some air holes 14.
The air sparger 11 is inserted perpendicularly into biological feedstuff, and biological feedstuff need to cover air hole 14, and topmost
The distance of air hole to biological feedstuff upper surface be setting value, setting value is depending on actual conditions, generally no less than the bed of material
/ 3rd of thickness, to prevent " short-circuit of air ".
In specific operation, by be inoculated with mixed bacteria biological feedstuff load round 12 (fermentation vat, fermenter or
Fermentation bag) in, it is inserted into according to round insertion air sparger pipe uniform in size, then by temperature sensor 9 in biological feedstuff,
The probe of temperature sensor 9 is located at biological feedstuff lower middle position, 10 time interval of setting time controller and duration of ventilation, if
Determine 7 temperature control scope of temp controlled meter, the outlet air control temperature of setting air heater 6, opens air compressor machine 1.
Ventilation temperature-controlling system provided in this embodiment, periodically can supply oxygen toward deep layer biological feedstuff and energy is intelligentized
Fermentation temperature is controlled, ensures the abundant fermentation of aerobic bacteria in biological feedstuff, improves product quality.
The present embodiment only illustrates technical scheme, rather than its limitations.
Embodiment 2
Weigh fresh dregs of beans 19kg and skin of beancurd 1kg to be uniformly mixed, crushed 10 mesh sieves and used as fermentation base-material.Using yeast
Bacterium NKY1, bacillus subtilis, lactobacillus plantarum and lactobacillus reuteri LR1 mixed bacterias ferment.Base-material fermentation moisture
Control need to prepare 13.33 liters of biological feedstuff fermentation mixed bacteria seed liquors containing mixed bacteria 40%.Weigh first
266.6g glucose, 40g K2HPO4With 26.7g KH2PO4Certain volume is added to being preheated in 30 DEG C of clear water, is sufficiently stirred
Dissolving.Saccharomycete and bacillus subtilis are activated with activating solution respectively.Then live toward adding a certain amount of saccharomycete in nutrient solution
Change liquid and bacillus subtilis activating solution, the saccharomycete in final zymotic fluid and bacillus subtilis viable count is respectively reached 2
×107cfu/mL.Lactobacillus plantarum and lactobacillus reuteri overnight culture are added afterwards, make the plant breast in final zymotic fluid
Bacillus and lactobacillus reuteri viable count respectively reach 2 × 107Cfu/mL, obtains bean pulp fermentation seed liquor.Alkaline egg is weighed again
White enzyme (20 × 104U/g) 5 grams, neutral proteinase (10 × 104U/g) 10 grams and acid protease (5 × 104U/g) 10 grams of additions
Into bean pulp fermentation seed liquor, add water to adjust to 13.33 liters, stir evenly that to can be used as biological feedstuff fermentation Mixed Microbes a variety of
Sub- liquid.The biological feedstuff fermentation of mixing is uniformly sprayed in fermentation base-material with mixed bacteria seed liquor, is stirred, will
Fermentation base-material after inoculation is transferred in fermenter, air sparger pipe and temperature sensor (PT100) is inserted into material, by sky
The outlet temperature of hot-air heater is arranged to 37 DEG C, and second solenoid valve switch is intermittent on passage time controller control air pipe line
Tempeerature-constant air is passed through into material, is set in earlier fermentation (before material temperature does not rise) at interval of 10 minutes toward being passed through 10 seconds in material
Tempeerature-constant air, sets in the fermentation materials material temperature rise middle and later periods and was passed through 10 seconds air at interval of 5 minutes.Control fermentation materials temperature
Between 32~37 DEG C, once fermentation materials more than 37 DEG C, by cooling down to material toward being passed through air in material.Ventilation early period hair
The ferment stage continues 36h, then stops ventilation and continues anaerobic fermentation 24h, obtains the biological feedstuff rich in probiotics, biological feedstuff can
Using low temperature drying or Direct-fed animal.
Indexs measure
A) count plate：The biological feedstuff for taking 3g wet is placed in 100mL sterile salines, is shaken 20min, is taken supernatant
Diluted with sterile water, take that the dilution of suitable multiple is respectively coated LB, YPD (addition ampicillin and streptomysin) and MRS is put down
Plate carries out count plate, counts each tablet viable count.
B) pH value measures：Weigh the wet biological feedstuffs of 10.0g to be placed in 100mL distilled water, shake 10min, take supernatant
PH value is surveyed with pH meter.
C) lactic acid content measures：The kit that biotech firm's offer is built up using Nanjing is detected.
D) determining the protein quantity：Biological feedstuff is sampled using Kjeldahl nitrogen determination after drying.
Table 1 ferments the index of correlation data of front and rear material
| Index | Before fermentation | After fermentation |
| Albumen (%) | 46.3% | 51.2% |
| Material pH value | 6.60 | 4.40 |
| Lactic acid content | 0 | 2.0% |
| Lactobacillus | / | 2.8×109 |
According to table 1, protein content improves after feed is fermented, and contains a large amount of probiotics in biological feedstuff.
Embodiment 3
Weigh fresh dregs of beans 47.5kg and skin of beancurd 2.5kg to be uniformly mixed, crushed 10 mesh sieves and used as fermentation base-material.Using
Saccharomycete NKY1, bacillus subtilis, lactobacillus plantarum and lactobacillus reuteri LR1 mixed bacterias ferment.Base-material ferments
Moisture control need to prepare 33.33 liters of biological feedstuff fermentation mixed bacteria seed liquors containing mixed bacteria 40%.Weigh first
666.5g glucose, 100g K2HPO4With 66.8g KH2PO4Certain volume is added to being preheated in 30 DEG C of clear water, is fully stirred
Mix dissolving.Saccharomycete and bacillus subtilis are activated with activating solution respectively.Then toward adding a certain amount of saccharomycete in nutrient solution
Activating solution and bacillus subtilis activating solution, respectively reach the saccharomycete in final zymotic fluid and bacillus subtilis viable count
2×107cfu/mL.Lactobacillus plantarum and lactobacillus reuteri overnight culture are added afterwards, make the plant in final zymotic fluid
Lactobacillus and lactobacillus reuteri viable count respectively reach 2 × 107Cfu/mL, obtains bean pulp fermentation seed liquor.Alkalescence is weighed again
Protease (20 × 104U/g) 12.5 grams, neutral proteinase (10 × 104U/g) 25 grams and acid protease (5 × 104U/g) 25 grams
It is added in bean pulp fermentation seed liquor, adds water to adjust to 33.33 liters, stirs evenly and can be used as biological feedstuff fermentation Mixed Microbes
Kind seed liquor.The biological feedstuff fermentation of mixing is uniformly sprayed in fermentation base-material with mixed bacteria seed liquor, is sufficiently stirred
It is even, the fermentation base-material after inoculation is transferred in fermenter, air sparger pipe and temperature sensor are inserted into material
(PT100), the outlet temperature of air heater is arranged to 37 DEG C, passage time controller controls the second electromagnetism on air pipe line
Threshold switch intermittence is passed through Tempeerature-constant air into material, is set in earlier fermentation (before material temperature does not rise) at interval of 10 minutes past things
10 seconds Tempeerature-constant airs are passed through in material, is set in the fermentation materials material temperature rise middle and later periods and was passed through 10 seconds air at interval of 5 minutes.Control
Fermentation materials temperature between 32~37 DEG C, once fermentation materials more than 37 DEG C, by being dropped to material toward being passed through air in material
Temperature.Ventilating fermentation stage early period continues 36h, then stops ventilation and continues anaerobic fermentation 24h, obtains the biology feeding rich in probiotics
Material, biological feedstuff can use and carry out low temperature drying or Direct-fed animal.
Each Indexs measure is with reference to embodiment 1.
Table 2 ferments the index of correlation data of front and rear material
| Index | Before fermentation | After fermentation |
| Albumen (%) | 46.3% | 50.9% |
| Material pH value | 6.60 | 4.35 |
| Lactic acid content | 0 | 2.18% |
| Viable count | / | 2.5×109 |
According to table 2, protein content improves after feed is fermented, and contains a large amount of probiotics in biological feedstuff.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of ventilation temperature-controlling system for biological feedstuff aerobic-anaerobic coupled fermentation, it is characterised in that filled including compressed air
Put, spinner flowmeter, air filter, air heater, fermentation temperature regulating device, venting in timing manner control device, air sparger,
Round；Filling biological feedstuff in round；
The blowing plant, spinner flowmeter, air filter, air heater, air sparger pass sequentially through pipe
Road is connected；
The fermentation temperature regulating device includes temperature sensor, temp controlled meter and the first solenoid valve, the temperature sensor and temperature
The connection of table signal is controlled, temp controlled meter is connected with the first solenoid signal；
The venting in timing manner control device includes time controller and second solenoid valve, the time controller and the second electricity
Magnet valve signal connects；
The air heater is connected by the first pipeline with first solenoid valve one end, and the first solenoid valve other end passes through second
Pipeline is connected with air sparger；
Described second solenoid valve one end is connected with the first pipeline, and the other end is connected with second pipe.
2. the ventilation temperature-controlling system for biological feedstuff aerobic-anaerobic coupled fermentation according to right will require 1, its feature
It is：
The blowing plant includes air compressor machine, air accumulator and cooling driers, the air compressor machine, air accumulator and cooling driers according to
It is secondary to be connected by pressure pipeline；
One end of the spinner flowmeter is connected with cooling driers, and the other end of spinner flowmeter is connected with air filter.
3. the ventilation temperature-controlling system for biological feedstuff aerobic-anaerobic coupled fermentation according to right will require 1, its feature
It is：
The air filter is pipelined filter, degerming filter core built in air filter；
The air heater is the heating unit with temperature control modules, and thermometric is provided with the gas outlet of air heater
Sensor；
The probe of the temperature sensor is located in biological feedstuff.
4. the ventilation temperature-controlling system for biological feedstuff aerobic-anaerobic coupled fermentation according to right will require 1, its feature
It is：
The air sparger is the round tube of lower end sealing, and the upper port of air sparger is connected with second pipe, air
The lower part of the pipe side wall of distributor is provided with some air holes；Air sparger has multiple；
The air sparger is inserted perpendicularly into biological feedstuff, and air hole is located in biological feedstuff, and uppermost air hole
Distance to biological feedstuff upper surface is setting value.
5. the ventilation temperature-controlling system for biological feedstuff aerobic-anaerobic coupled fermentation according to right will require 1, its feature
It is：
The round is the large-scale shedding motion of sealed bottom.
A kind of 6. method of aerobic-anaerobic coupled fermentation production biological feedstuff, it is characterised in that include the following steps：
(1) pretreatment of raw material
Select without mouldy, free from extraneous odour and without rotten dregs of beans, skin of beancurd as fermentation raw material, dregs of beans dosage is 90%~100%, beans
Skin dosage is 0~10%, and raw material is uniformly mixed and is used as fermentation base-material through pulverizing and sieving by raw material according to formula；
(2) prepare biological feedstuff fermentation and use mixed bacteria seed liquor
By the saccharomycete activated (Saccharomyces cerevisiae), bacillus subtilis (Bacillus
Subtilis), lactobacillus plantarum (Lactobacillus plantarum) and lactobacillus reuteri (Lactobacillus
Reuteri) additive amount is poured into nutrient solution strain on demand, is thoroughly mixed uniformly, nutrient solution water temperature is controlled 30 ± 1
DEG C, this mixed bacteria liquid is bean pulp fermentation seed liquor；Again toward addition acid protease, neutral proteinase in bean pulp fermentation seed liquor
And alkali protease, each protease additive amount is on the basis of the base-material that ferments, i.e.,：25~100U/g of alkali protease additive amount；In
25~100U/g of property protease additive amount；Acidic protein enzyme amount 10~50U/g of additive amount, weighs each protease and is sent out in right amount with dregs of beans
Ferment seed liquor is that mixed bacteria seed liquor is used in biological feedstuff fermentation after mixing；
(3) base-material ferments
The biological feedstuff fermentation described in step (2) is uniformly sprayed into the fermentation base-material described in step (1) and uses mixed bacteria seed
Liquid, control base-material moisture is between 35%~45%, and base-material is sufficiently mixed uniformly with mixed bacteria seed liquor, the hair that will be inoculated with
Ferment base-material is encased in the ventilation temperature control system that Claims 1 to 5 any one of them is used for biological feedstuff aerobic-anaerobic coupled fermentation
In the round of system, base-material ulking thickness is inserted into air sparger pipe, at the same time to be advisable no more than 100cm in fermentation materials
Thermometric temperature sensor is inserted into, it is 32~37 DEG C to control fermentation temperature, and ferment 36h~48h under controlled temperature conditions of divulging information, and then detests
24~48h of aerobe fermentation, obtains biological feedstuff.
7. the method for aerobic-anaerobic coupled fermentation production biological feedstuff according to claim 6, it is characterised in that：
In step (1), the sieving was 10 mesh sieves；
In step (2), the mass percent meter of component contained by the nutrient solution is as follows：Glucose or sucrose 2%, K2HPO4
0.3% and KH2PO40.2%.
8. the method for aerobic-anaerobic coupled fermentation production biological feedstuff according to claim 6, it is characterised in that：
In step (2), saccharomycete and bacillus content are in the bean pulp fermentation seed liquor：Saccharomycete 1 × 107~3 ×
107cfu/mL；Bacillus subtilis 1 × 107~5 × 107cfu/mL；Lactobacillus plantarum 1 × 107~3 × 107cfu/mL；Roy
Family name's lactobacillus 1 × 107~3 × 107cfu/mL。
9. the method for aerobic-anaerobic coupled fermentation production biological feedstuff according to claim 6, it is characterised in that：
In step (3), the air outlet temperature of air heater is arranged to 37 DEG C, passage time controller is controlled on air pipe line
Second solenoid valve switch intermittence is passed through Tempeerature-constant air into material, is set in earlier fermentation at interval of 10 minutes toward logical in material
Enter 5~15 seconds Tempeerature-constant airs, set in the fermentation materials material temperature rise middle and later periods and be passed through 5~15 seconds air at interval of 5 minutes；Control
Fermentation materials temperature between 32~37 DEG C, once fermentation materials more than 37 DEG C, by being dropped to material toward being passed through air in material
Temperature.
10. a kind of biological feedstuff, it is characterised in that ferment to obtain by claim 6~9 any one of them method.
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| CN108497158A (en) * | 2018-06-11 | 2018-09-07 | 厦门市科环海洋生物科技有限公司 | A kind of the biological and ecological methods to prevent plant disease, pests, and erosion fermented feed and its production method and production equipment of prevention vibriosis |
| CN109294889A (en) * | 2018-08-31 | 2019-02-01 | 南通劲凌智能科技有限公司 | Probiotics fermented beverage fermentation tank, component proportion and its process |
| CN110353088A (en) * | 2019-07-18 | 2019-10-22 | 广东省农业科学院动物科学研究所 | A kind of preparation method of fermented bean dregs |
| CN113046228A (en) * | 2021-04-01 | 2021-06-29 | 江苏罗迈特生物科技有限公司 | Wine mother expansion culture system |
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| CN109294889B (en) * | 2018-08-31 | 2021-10-15 | 杭州百芮生物科技有限公司 | Probiotic fermented beverage fermentation tank, component proportion and process method thereof |
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| CN113046228A (en) * | 2021-04-01 | 2021-06-29 | 江苏罗迈特生物科技有限公司 | Wine mother expansion culture system |
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