CN105166458A - Fermented selenium-rich plant feed additive resistant to pig heat stress - Google Patents
Fermented selenium-rich plant feed additive resistant to pig heat stress Download PDFInfo
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Abstract
The present invention discloses a fermented selenium-rich plant feed additive resistant to pig heat stress and a preparation method of the feed additive comprises the following three steps: (1) preparation of selenium-rich yeast culture medium; (2) preparation of traditional Chinese medicine fermentation culture medium; and (3) traditional Chinese medicine fermentation. The plant feed additive is characterized in that: the traditional Chinese medicine fermentation culture medium obtained in the step 2 includes (2.1) preparation of Chinese herbal medicine probiotics and (2.2) inoculation steps. The designed fermented selenium-rich plant feed additive is a pig heat stress resistant novel feed additive which is green and safe, contains traditional Chinese medicines, and combines organic selenium. The feed additive fully integrates the physiological characteristics of heat-stressed pigs, uses anti-oxidative stress as main features, can significantly improve the pig weight gain and feed-weight ratio in a high-temperature environment, and enhance pig anti-oxidation resistance and immune function after being fed, effectively plays the roles of pig stress resistance, and has non-toxic or side effects and no harmful substance residues after a long-term use.
Description
Technical field
The present invention relates to a kind of feed addictive, especially a kind of fermented type selenium-enriched plant feed addictive that can resist pig heat stress.
Background technology
Most area summer of south China and high, the high humidity of temperature early autumn, the central and north in recent years summer temperature is also very high, add and as developed country, do not adopt the totally enclosed pig house of temperature and humidity control, therefore the heat stress problem of pig is more and more serious, creates great impact especially on the pig under intensive high-density rearing.Heat stress not only affects behavior and the production performance of live pig, and all causes very large impact to the immunologic function of pig, fertility and meat quality etc.Therefore, how to slow down the harm of heat stress to Swine Production, the production performance improved under its hot conditions has become current pig industry problem demanding prompt solution.In the exploitation of anti-heat stress agent, some anti-heat stress medicine Long-Time Service existing, be difficult to avoid occurring toxic and side effect and medicament residue, and dosage is also difficult to grasp, and some conventional heat stress fighting additives (as VC), easy oxidized inefficacy in adding procedure, final can have how much to be absorbed by body be difficult to estimate, final effect is not ideal, also has the medicine of some anti-heat stress, there is toxic and side effect and medicament residue unavoidably after Long-Time Service, do not meet modern food safety requirements.Therefore, need to invent a kind of have safe and harmless, without the fermented type selenium-enriched plant feed addictive that can resist pig heat stress of the resistance to the action of a drug, drug residue free, the feature such as to have no side effect.
Summary of the invention
The object of the invention is the deficiency in order to solve above-mentioned technology and design a kind of green, safety, the fermented type selenium-enriched plant feed addictive that can resist pig heat stress that combines containing fermented tcm and Organic Selenium.
The fermented type selenium-enriched plant feed addictive that can resist pig heat stress designed by the present invention, its preparation method comprises following three steps: the preparation of (1) Se-enriched yeast nutrient solution, (2) herb fermenting medium preparing, (3) herb fermenting, is characterized in that:
(1) Se-enriched yeast nutrient solution preparation: Fructus Hordei Germinatus: water=1:4 (weight ratio), at 55-60 DEG C of insulation saccharification 3-4h, filter and boil filtrate, cooling clarification, the pre-sterilized sodium selenite solution of 10% (weight ratio) is added in brewer's wort, sodium selenite solution concentration is 5mg/ml, access saccharomyces cerevisiae and lactobacillus acidophilus;
(2) herb fermenting medium preparing: comprise step prepared by (2.1) Chinese herbal medicine probiotic bacteria culture medium and the step that (2.2) are inoculated, wherein:
(2.1) preparation of Chinese herbal medicine probiotic bacteria culture medium: mixed with the nutrient salt solution of microorganism after pretreatment by solid fermentation raw materials, sterilizing, obtains Chinese herbal medicine probiotic bacteria culture medium;
Wherein, described solid state fermentation raw material is grouped into by each one-tenth of following weight portion: hawthorn 8-10 part, rhizoma atractylodis 10-12 part, dried orange peel 10-12 part, betel nut 5-8 part, root of large-flowered skullcap 8-12 part, wilsonii 10-14 part, Radix Glycyrrhizae 8-12, fruit of Chinese magnoliavine 8-12 part, felwort 8-10 part, Radix Isatidis 10-12 part, perilla leaf 10-12 part, dried peppermint leaf 10-12 part; Above-mentioned each tcm components material is chosen in described pretreatment, after drying 1 ~ 2 hour at 50 ~ 60 DEG C, pulverizes, crosses 60 eye mesh screens, for subsequent use;
Composition and the quality of described microbial nutrition salting liquid are:
Glucose 20.0-22.0g;
Sodium acetate trihydrate 5.0-5.2g;
Triammonium citrate 2.0-2.3g;
K
2HPO
42.0-2.2g；
MgSO
4·7H
2O0.2-0.3g；
MnSO
4·4H
2O0.05-0.06g；
Distilled water 1000-1200mL;
Wherein, the pH value of described microbial nutrition salting liquid is 6.5;
Solid state fermentation raw material is mixed by the ratio of 1g:2.0mL with the nutrient salt solution of microorganism;
(2.2) inoculate: with probio lactobacillus bulgaricus for bacterial classification, bacterial classification is expanded after cultivating and be inoculated into
(2.1) in the Chinese herbal medicine probiotic bacteria culture medium prepared by step thus obtained described herb fermenting culture medium; It should be noted that: lactobacillus bulgaricus is known bacterial classification, and bacterial classification is commercially available;
(3) herb fermenting:
Se-enriched yeast nutrient solution prepared by step (1) and the herb fermenting culture medium prepared by step (2) are mixed, 35 ~ 37 DEG C of closing anaerobic fermentations 7 ~ 10 days, stirred once every 24 hours;
Tunning post processing; Fermentate after being completed by anaerobic fermentation is under 35 ~ 50 DEG C of conditions after drying, and pulverize, be up to the standards packaging.
As preferably, described (2.2) inoculation comprises the steps:
A, with liquid seeds inoculation, level liquid seed culture adopts MRS culture medium, and temperature 37 DEG C, under the condition of rotating speed 80r/min, 24h cultivated by shaking table; Cultured primary seed solution is inoculated in secondary seed fermentation tank and carries out expansion cultivation, reach 26 ~ 32 hours and stop cultivating, thus obtain total bacterium liquid, for subsequent use;
B, Chinese herbal medicine probiotic bacteria culture medium obtained to (2.1) to be mixed, add the water of 1 ~ 1.2 times of weight accounting for Chinese herbal medicine probiotic bacteria culture medium gross weight, stir, add the total bacterium liquid measure accounting for Chinese herbal medicine probiotic bacteria culture medium gross weight 10%.
The fermented type selenium-enriched plant feed addictive that can resist pig heat stress designed by the present invention, it is a kind of green, safety, the novel fodder additive of anti-pig heat stress that combines containing fermented tcm and Organic Selenium, this feed addictive is fully in conjunction with the physilogical characteristics of heat stress pig, take anti-oxidative stress as main feature, after feeding, significantly can improve weightening finish and the feed-weight ratio of Pigs Exposed To Heat, strengthen antioxygenic property and the immunologic function of pig, the effect of effective performance pig resisting stress, and it is residual with unharmful substance to have no side effect after Long-Time Service.Fermentable is carried out after maximum feature of the present invention is composite by science by plurality of Chinese composition exactly, achieve the organic assembling of compound probiotic, Chinese herbal and crude drugs preparations and selenium element three, give full play to the feature strengthening the oxidation resistance of body, solve pig under thermal environment stress problem, and can effectively improve pig immunologic function, reduce M & M, thus improve production performance and the culture benefit of weanling pig.And there is not drug resistance problems, produce meat meat good, final production goes out the non-harmful pork of high-quality.
Detailed description of the invention
The present invention is further described by the following embodiment.
Embodiment 1:
A kind of fermented type selenium-enriched plant feed addictive that can resist pig heat stress described by the present embodiment, its preparation method comprises following three steps: the preparation of (1) Se-enriched yeast nutrient solution, (2) herb fermenting medium preparing, (3) herb fermenting, is characterized in that:
(1) Se-enriched yeast nutrient solution preparation: Fructus Hordei Germinatus: water=1:4 (weight ratio), at 55 DEG C of insulation saccharification 3h, filter and boil filtrate, cooling clarification, the pre-sterilized sodium selenite solution of 10% (weight ratio) is added in brewer's wort, sodium selenite solution concentration is 5mg/ml, access saccharomyces cerevisiae and lactobacillus acidophilus;
(2) herb fermenting medium preparing: comprise step prepared by (2.1) Chinese herbal medicine probiotic bacteria culture medium and the step that (2.2) are inoculated, wherein:
(2.1) preparation of Chinese herbal medicine probiotic bacteria culture medium: mixed with the nutrient salt solution of microorganism after pretreatment by solid fermentation raw materials, sterilizing, obtains Chinese herbal medicine probiotic bacteria culture medium;
Wherein, described solid state fermentation raw material is grouped into by each one-tenth of following weight portion: hawthorn 8 parts, rhizoma atractylodis 10 parts, dried orange peel 10 parts, betel nut 5 parts, the root of large-flowered skullcap 8 parts, wilsonii 10 parts, Radix Glycyrrhizae 8,8 parts, the fruit of Chinese magnoliavine, felwort 8 parts, Radix Isatidis 10 parts, perilla leaf 10 parts, dried peppermint leaf 10 parts; Above-mentioned each tcm components material is chosen in described pretreatment, after drying 1 hour at 50 DEG C, pulverizes, crosses 60 eye mesh screens, for subsequent use;
Composition and the quality of described microbial nutrition salting liquid are:
Glucose 20.0g;
Sodium acetate trihydrate 5.0g;
Triammonium citrate 2.0g;
K
2HPO
42.0g；
MgSO
4·7H
2O0.2g；
MnSO
4·4H
2O0.05g；
Distilled water 1000-1200mL, makes the pH value of described microbial nutrition salting liquid be 6.5;
Solid state fermentation raw material is mixed by the ratio of 1g:2.0mL with the nutrient salt solution of microorganism;
(2.2) inoculate: with probio lactobacillus bulgaricus for bacterial classification, bacterial classification to be expanded in the Chinese herbal medicine probiotic bacteria culture medium being inoculated into prepared by (2.1) step after cultivating thus obtained described herb fermenting culture medium; Described (2.2) inoculation comprises the steps:
A, with liquid seeds inoculation, level liquid seed culture adopts MRS culture medium, and temperature 37 DEG C, under the condition of rotating speed 80r/min, 24h cultivated by shaking table; Cultured primary seed solution is inoculated in secondary seed fermentation tank and carries out expansion cultivation, reach 26 hours and stop cultivating, thus obtain total bacterium liquid, for subsequent use;
B, Chinese herbal medicine probiotic bacteria culture medium obtained to (2.1) to be mixed, add the water of the 1 times of weight accounting for Chinese herbal medicine probiotic bacteria culture medium gross weight, stir, add the total bacterium liquid measure accounting for Chinese herbal medicine probiotic bacteria culture medium gross weight 10%;
(3) herb fermenting:
Se-enriched yeast nutrient solution prepared by step (1) and the herb fermenting culture medium prepared by step (2) are mixed, ferments 7 days at 35 DEG C of closing anaerobics, stirred once every 24 hours;
Tunning post processing; Fermentate after being completed by anaerobic fermentation is under 35 DEG C of conditions after drying, and pulverize, be up to the standards packaging.
Effect is discussed further below by zoopery;
Feeding and management:
1, select 100 young pigs as feeding object, young pig body weight, at 34.5kg-35.5kg, is divided into two groups at random, i.e. control group and interpolation group, one group 50; This test is carried out, temperature 33-38 DEG C at Lv Yuan pig farm, Huaian district, Huai'an high temperature season August (on August-30 on the 1st);
2, control group and interpolation group feed intake three times every day, every day 6:00,12:00,17:30 respectively once, basal diet: Huaian Zheng Chang feed corporation,Ltd 4003 type mixed feed; Control group feeding basal diet, interpolation group feeding: the fermented type selenium-enriched plant feed addictive that can resist pig heat stress described by basal diet+0.5% the present embodiment.
3, measure August 20, August 25, August 30 this control group and interpolation group in three days indices, comprise body temperature, day increment and daily ingestion amount and blood in stress hormone ACTH level.
Wherein, body temperature: insert pig rectum 6cm depths with digital clinical thermometer and measure temperature;
Blood specimen collection and preparation: the disposable syringe of empty stomach 20ml gathered ear vein blood and was about 10ml morning, detected stress hormone ACTH level in blood.
Measure number acute as follows:
Table one, each item number play on August 20 statistics
| Project | Control group | Interpolation group |
| Mean body temperature (DEG C) | 39.8 | 39.6 |
| Most high fever (DEG C) | 40.2 | 39.9 |
| Minimum body temperature (DEG C) | 39.6 | 39.4 |
| Average day increment g/d | 755 | 810 |
| Average daily ingestion amount g/d | 1769 | 1760 |
| Average A CTH (pg/ml) | 0.95 | 0.86 |
| The highest ACTH (pg/ml) | 0.96 | 0.87 |
| Minimum ACTH (pg/ml) | 0.94 | 0.84 |
Table two, each item number play on August 25 statistics
| Project | Control group | Interpolation group |
| Mean body temperature (DEG C) | 40.3 | 39.5 |
| Most high fever (DEG C) | 40.7 | 39.9 |
| Minimum body temperature (DEG C) | 39.7 | 39.2 |
| Average day increment g/d | 805 | 861 |
| Average daily ingestion amount g/d | 1930 | 1931 |
| Average A CTH (pg/ml) | 0.96 | 0.85 |
| The highest ACTH (pg/ml) | 0.97 | 0.86 |
| Minimum ACTH (pg/ml) | 0.94 | 0.84 |
Table three, each item number play on August 30 statistics
| Project | Control group | Interpolation group |
| Mean body temperature (DEG C) | 40.6 | 39.5 |
| Most high fever (DEG C) | 40.9 | 39.8 |
| Minimum body temperature (DEG C) | 40.1 | 39.1 |
| Average day increment g/d | 846 | 896 |
| Average daily ingestion amount g/d | 2056 | 2053 |
| Average A CTH (pg/ml) | 0.97 | 0.84 |
| The highest ACTH (pg/ml) | 0.98 | 0.85 |
| Minimum ACTH (pg/ml) | 0.95 | 0.83 |
From above-mentioned three tables,
1, interpolation group body temperature is lower than control group, and along with passage of time control group body temperature totally raises gradually, interpolation group body temperature is stablized, and slightly declines;
2, when average daily ingestion amount is substantially equal, interpolation group average day increment be greater than control group;
3, in blood, stress hormone ACTH level interpolation group body temperature is lower than control group, and along with passage of time control group A CTH level raises gradually, interpolation group ACTH horizontal stable, slightly declines.
It can thus be appreciated that the fermented type selenium-enriched plant feed addictive of the present embodiment can resist pig heat stress, significantly can improve the weightening finish of Pigs Exposed To Heat simultaneously.Analyze from principle, herb fermenting is according to microecology and bionics theory, by biological grafting method, under the condition of analog machine animal body gastroenteric environment, the new medicine that biology is transformed is carried out to traditional Chinese medicine fine powder, at antibiotics resistance, under the situation that superbacteria constantly occurs, significant.In the present embodiment, solid fermentation series does carrier with traditional Chinese medicine powder, ferment with corresponding viable bacteria, to diarrhoea, the intestines problems such as punishment in advance, effect is better, after fermentable process, the cellulase that microorganism produces in metabolic process, the multiple ectoenzyme such as pectase enters culture medium, thus cause Chinese medicine cell rupture, its active ingredient is come out, and research shows, in sweat, it is easily the Small molecular active principle of animal absorption that the large molecule active principle not easily absorbed for animal can be degraded to by the various enzymes that microorganism produces, multiple large molecular impurity can be removed simultaneously, thus enhancing drug effect, fermentation even through microorganism produces new active material, so there is the effect described in above-mentioned table.
It should be noted that: the MRS culture medium that described inoculation step adopts is prior art, certainly also can adopt other lactobacillus bulgaricus culture mediums existing, as adopted culture medium composed as follows:
Peptone 10.0g; Beef extract 10.0g; Yeast extract 5.0g; Diammonium hydrogen citrate 2.0g; Glucose 20.0g; Tween 80 1.0mL; Sodium acetate 5.0g; Dipotassium hydrogen phosphate 2.0g; Magnesium sulfate 0.58g; Manganese sulfate 0.25g; Agar 18.0g; Distilled water 1000mL; PH6.2 ~ 6.6.
Embodiment 2:
A kind of fermented type selenium-enriched plant feed addictive that can resist pig heat stress described by the present embodiment, its preparation method comprises following three steps: the preparation of (1) Se-enriched yeast nutrient solution, (2) herb fermenting medium preparing, (3) herb fermenting, is characterized in that:
(1) Se-enriched yeast nutrient solution preparation: Fructus Hordei Germinatus: water=1:4 (weight ratio), at 60 DEG C of insulation saccharification 4h, filter and boil filtrate, cooling clarification, the pre-sterilized sodium selenite solution of 10% (weight ratio) is added in brewer's wort, sodium selenite solution concentration is 5mg/ml, access saccharomyces cerevisiae and lactobacillus acidophilus;
(2) herb fermenting medium preparing: comprise step prepared by (2.1) Chinese herbal medicine probiotic bacteria culture medium and the step that (2.2) are inoculated, wherein:
(2.1) preparation of Chinese herbal medicine probiotic bacteria culture medium: mixed with the nutrient salt solution of microorganism after pretreatment by solid fermentation raw materials, sterilizing, obtains Chinese herbal medicine probiotic bacteria culture medium;
Wherein, described solid state fermentation raw material is grouped into by each one-tenth of following weight portion: hawthorn 10 parts, rhizoma atractylodis 12 parts, dried orange peel 12 parts, betel nut 8 parts, the root of large-flowered skullcap 12 parts, wilsonii 14 parts, Radix Glycyrrhizae 12,12 parts, the fruit of Chinese magnoliavine, felwort 10 parts, Radix Isatidis 12 parts, perilla leaf 12 parts, dried peppermint leaf 12 parts; Above-mentioned each tcm components material is chosen in described pretreatment, after drying 2 hours at 60 DEG C, pulverizes, crosses 60 eye mesh screens, for subsequent use;
Composition and the quality of described microbial nutrition salting liquid are:
Glucose 22.0g;
Sodium acetate trihydrate 5.2g;
Triammonium citrate 2.3g;
K
2HPO
42.2g；
MgSO
4·7H
2O0.3g；
MnSO
4·4H
2O0.06g；
Distilled water 1000-1200mL; The pH value of described microbial nutrition salting liquid is made to be 6.5;
Solid state fermentation raw material is mixed by the ratio of 1g:2.0mL with the nutrient salt solution of microorganism;
(2.2) inoculate: with probio lactobacillus bulgaricus for bacterial classification, bacterial classification to be expanded in the Chinese herbal medicine probiotic bacteria culture medium being inoculated into prepared by (2.1) step after cultivating thus obtained described herb fermenting culture medium; Wherein: inoculation specifically comprises the steps:
A, with liquid seeds inoculation, level liquid seed culture adopts MRS culture medium, and temperature 37 DEG C, under the condition of rotating speed 80r/min, 24h cultivated by shaking table; Cultured primary seed solution is inoculated in secondary seed fermentation tank and carries out expansion cultivation, reach 32 hours and stop cultivating, thus obtain total bacterium liquid, for subsequent use;
B, Chinese herbal medicine probiotic bacteria culture medium obtained to (2.1) to be mixed, add the water of the 1.2 times of weight accounting for Chinese herbal medicine probiotic bacteria culture medium gross weight, stir, add the total bacterium liquid measure accounting for Chinese herbal medicine probiotic bacteria culture medium gross weight 10%.
(3) herb fermenting:
Se-enriched yeast nutrient solution prepared by step (1) and the herb fermenting culture medium prepared by step (2) are mixed, ferments 10 days at 37 DEG C of closing anaerobics, stirred once every 24 hours;
Tunning post processing; Fermentate after being completed by anaerobic fermentation is under 50 DEG C of conditions after drying, and pulverize, be up to the standards packaging, obtains finished product.
Effect is discussed further below by zoopery;
1 materials and methods
1.1 experimental animals and feeding and management
(Du × long × large) tri-crossbreeding of 36 about body weight 10kg is chosen in test, according to the principle of " parity is consistent, kind is identical, body weight is close, male and female half and half ".Often repeat separated raising.The phase of raising is one week in advance, and the formal test phase divides front and back two benches, the first stage is weanling pig stage (10 ~ 20kg stage), and second stage is grower pigs stage (20 ~ 50kgkg stage).Test pig adopts floor husbandry, semi-open pig house, gravity-flow ventilation.Pig free choice feeding and drinking-water.Immunity and expelling parasite program are undertaken by pig farm conventional synchronous.Observe at any time, record and search for food and health status.
1.2 test grouping and daily rations
Adopt the design of single-factor randomized block experiment, be divided into 2 processed group, i.e. interpolation group and control group, often group establishes 3 repetitions, each repetition 6 pigs.Concrete process is as follows, control group fed basal diet (not containing antibiotic etc), the fermented type selenium-enriched plant feed addictive described in the present embodiment of daily ration+0.75% based on test group.
1.3 feeding and management
This test is carried out at Huaian district, Huai'an Lv Yuan pig farm high temperature season (7 one October), and in test house, room temperature is up to 38.5 DEG C, minimum temperature 23 DEG C, mean temperature 31 DEG C, medial humidity 65%.Test is carried out in same pig house, beats overbit when raising beginning in advance to test pig.
1.4 feed intake drinking-water
Feed is powder, and fermented type selenium-enriched plant feed addictive adds in 0.75% ratio of feed.Feeding method adopts subfield to feed, piglet stage four times a day (6:00,10:00,16:00,20:00 respectively feed intake once), middle every day in pig stage three (6:00,11:00,17:00 respectively feeds intake once), feed intake needs to get certain forage volume charging basket one to one from often enclosing according to searching for food of often enclosing at every turn, records each charge weight of charging basket often enclosed; Adopt stage diet before and after mixing before entering preliminary trial period and test second stage, 3 ~ 4d is transition gradually.Drinking-water adopts water fountain freely to drink water.All the other are with conventional feeding and management.
1.3 testing indexs and method
1.3.1 the mensuration of body temperature
In test the 1st, 3,6,10d8:00AM, often select 6 pigs (often repeating two) in group, with digital clinical thermometer insert rectum 6cm depths measure temperature (resolution ratio 0.1 DEG C)
1.3.2 production performance
In units of circle, record examination every day pig feed consumption rate, adds up the feed often enclosed in per stage, calculates total amount as the stage total feed consumption rate (kg); It is stage total augment weight with the difference of starting weight (kg) that every every step-by-step test of test pig terminates end heavy (kg), average daily ingestion amount (AverageDailyFeedIntake is calculated respectively by following formula, ADFI), daily gain (AverageDailyGain, and feed conversion rate (FeedConversionRate, FCR) ADG);
ADFI=stage total feed consumption rate (kg)/stage number of days (d)
ADG=stage total augment weight (kg)/stage number of days (d)
FCR=stage total augment weight (kg)/stage total feed consumption rate (kg)
1.3.3 blood specimen collection and preparation
At the end of first and second stage of test, morning, the disposable syringe collection ear vein blood of empty stomach 20ml was about 10ml, leave standstill after about 30 minutes, adopt the centrifugation 10min of 4500r/min, prepare blood serum sample, be sub-packed in EPPendorf pipe, to be measured in-20 DEG C of Refrigerator stores after packing.Wherein, serum total number born (SOD) activity adopts SOD hydroxylamine assay, glutathione peroxidase (GSH-Px) activity adopts DTNB direct method, and MDA (MDA) content adopts TBA method, and These parameters all adopts commercial reagents box to measure.Serum corticotropin (ACTH) and cortisol (Cor) adopt radioimmunology (to adopt
125i marks).
1.4 data processing
Test the data obtained, through Excel process, adopts SPSS9.0 software GLM program to carry out variance analysis, adopts Duncan to carry out Multiple range test.
2 result of the tests
2.1 clinical observation result
The main manifestations of high temperature group pig is, hotly pants, and body temperature raises slightly, and feed intake declines, happiness drinking-water, and activity reduces, and upholds body, drowsiness, minimizing of urinating, argol etc.; Fermented type selenium-enriched plant feed addictive group is significantly improved than high temperature group.
The change of 2.2 temperature of pig body
As can be seen from Table 1, fermented type selenium-enriched plant feed addictive group temperature of pig body at 10d significantly lower than high temperature group (P<0.05); Compare with between each sky of group, high temperature group body temperature has the trend raised gradually, and wherein 6d is significantly higher than 1d (P<0.05), all without significant difference between each sky of fermented type selenium-enriched plant feed addictive group.
The change of table 1 temperature of pig body
Note:
a-bin same row, data shoulder mark is different represents significant difference (P<0.05), represents difference not significantly extremely significantly (P>0.05) without letter or same letter.Following table is same.
a-Brepresent significant difference (P<0.05) with data shoulder mark is different in a line, represent difference not significantly extremely significantly (P>0.05) without letter or same letter.Following table is same.
The change of 2.3 pig production performances
From table 2, in 10 ~ 20kg stage of pig growth, add fermented type selenium-enriched plant feed addictive in daily ration and significantly can increase daily gain (P<0.05), in 10 ~ 20kg stage of pig growth, add fermented type selenium-enriched plant feed addictive in daily ration and significantly can increase daily gain 7.04% and stage average weight 9.92% (P<0.05), and significantly improve feedstuff-meat ratio (P<0.05).
Table 2 production performance contrast table
Note:
a-brepresent significant difference (P<0.05) with data shoulder mark is different in a line, represent difference not significantly extremely significantly (P>0.05) without letter or same letter.
2.4 Swine serum anti-oxidation functions and stress the change of index
From table 3, at the end of the first experimental stage, compared with control group, test interpolation group can increase the activity of GSH-Px to a certain extent, reduces the content of serum MDA, but difference is not significantly (P>0.05); Compared with control group, the SOD activity of test interpolation group has remarkable rising (P<0.05), and simultaneously, in stress hormone, the level of ACTH and Cor significantly reduces (P<0.01).
At the end of the second experimental stage, compared with control group, test interpolation group significantly can increase the activity (P<0.05) of SOD and GSH-Px, significantly reduces the level (P<0.05) of serum MDA, stress hormone ACTH and Cor.
Table 3 serum anti-oxidation function and stress index
The impact of whole experimental stage high temperature on animal anti-oxidation function is that early stage is larger than later stage, and in serum, corticotropin and cortisol levels variation tendency are all obviously decline after first rising.And the anti-oxidation function of fermented type selenium-enriched plant feed addictive group and stress hormone remain on more stable level always, the change of front and back phase is little.This illustrate, fermented type selenium-enriched plant feed addictive of the present invention have good opposing summer fever stress effect.
Claims (2)
1. one kind can resist the fermented type selenium-enriched plant feed addictive of pig heat stress, its preparation method comprises following three steps: the preparation of (1) Se-enriched yeast nutrient solution, (2) herb fermenting medium preparing, (3) herb fermenting, is characterized in that:
(1) Se-enriched yeast nutrient solution preparation: Fructus Hordei Germinatus: water=1:4 (weight ratio), at 55-60 DEG C of insulation saccharification 3-4h, filter and boil filtrate, cooling clarification, the pre-sterilized sodium selenite solution of 10% (weight ratio) is added in brewer's wort, sodium selenite solution concentration is 5mg/ml, access saccharomyces cerevisiae and lactobacillus acidophilus;
(2) herb fermenting medium preparing: comprise step prepared by (2.1) Chinese herbal medicine probiotic bacteria culture medium and the step that (2.2) are inoculated, wherein:
(2.1) preparation of Chinese herbal medicine probiotic bacteria culture medium: mixed with the nutrient salt solution of microorganism after pretreatment by solid fermentation raw materials, sterilizing, obtains Chinese herbal medicine probiotic bacteria culture medium;
Wherein, described solid state fermentation raw material is grouped into by each one-tenth of following weight portion: hawthorn 8-10 part, rhizoma atractylodis 10-12 part, dried orange peel 10-12 part, betel nut 5-8 part, root of large-flowered skullcap 8-12 part, wilsonii 10-14 part, Radix Glycyrrhizae 8-12, fruit of Chinese magnoliavine 8-12 part, felwort 8-10 part, Radix Isatidis 10-12 part, perilla leaf 10-12 part, dried peppermint leaf 10-12 part; Above-mentioned each tcm components material is chosen in described pretreatment, after drying 1 ~ 2 hour at 50 ~ 60 DEG C, pulverizes, crosses 60 eye mesh screens, for subsequent use;
Composition and the quality of described microbial nutrition salting liquid are:
Glucose 20.0-22.0g;
Sodium acetate trihydrate 5.0-5.2g;
Triammonium citrate 2.0-2.3g;
K
2HPO
42.0-2.2g；
MgSO
4·7H
2O0.2-0.3g；
MnSO
4·4H
2O0.05-0.06g；
Distilled water 1000-1200mL;
Wherein, the pH value of described microbial nutrition salting liquid is 6.5;
Solid state fermentation raw material is mixed by the ratio of 1g:2.0mL with the nutrient salt solution of microorganism;
(2.2) inoculate: with probio lactobacillus bulgaricus for bacterial classification, bacterial classification to be expanded in the Chinese herbal medicine probiotic bacteria culture medium being inoculated into prepared by (2.1) step after cultivating thus obtained described herb fermenting culture medium;
(3) herb fermenting:
Se-enriched yeast nutrient solution prepared by step (1) and the herb fermenting culture medium prepared by step (2) are mixed, 35 ~ 37 DEG C of closing anaerobic fermentations 7 ~ 10 days, stirred once every 24 hours;
Tunning post processing; Fermentate after being completed by anaerobic fermentation is under 35 ~ 50 DEG C of conditions after drying, and pulverize, be up to the standards packaging.
2. the fermented type selenium-enriched plant feed addictive that can resist pig heat stress according to claim 1, is characterized in that described (2.2) inoculation comprises the steps:
A, with liquid seeds inoculation, level liquid seed culture adopts MRS culture medium, and temperature 37 DEG C, under the condition of rotating speed 80r/min, 24h cultivated by shaking table; Cultured primary seed solution is inoculated in secondary seed fermentation tank and carries out expansion cultivation, reach 26 ~ 32 hours and stop cultivating, thus obtain total bacterium liquid, for subsequent use;
B, Chinese herbal medicine probiotic bacteria culture medium obtained to (2.1) to be mixed, add the water of 1 ~ 1.2 times of weight accounting for Chinese herbal medicine probiotic bacteria culture medium gross weight, stir, add the total bacterium liquid measure accounting for Chinese herbal medicine probiotic bacteria culture medium gross weight 10%.
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| CN112868927A (en) * | 2021-02-01 | 2021-06-01 | 广东省农业科学院动物科学研究所 | Chinese herbal medicine and probiotic compound feed additive for relieving pig stress and improving meat quality and application thereof |
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