CN101116473A - Method for preparing glycolysis Chinese herbal medicine preparations for feed - Google Patents
Method for preparing glycolysis Chinese herbal medicine preparations for feed Download PDFInfo
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- CN101116473A CN101116473A CNA2006100696282A CN200610069628A CN101116473A CN 101116473 A CN101116473 A CN 101116473A CN A2006100696282 A CNA2006100696282 A CN A2006100696282A CN 200610069628 A CN200610069628 A CN 200610069628A CN 101116473 A CN101116473 A CN 101116473A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
A preparation method of a ferment Chinese Traditional Herbs for feeding belongs to the production field of the feed additives. The technical proposal provided by the present invention is that one or various intestinal probiotics is inoculated on a cultivation matrix containing extracts of Chinese Traditional Herbs, and undergoes ferment cultivation in a proper condition. The probiotics include Lactobacillus acidophilus, Bacillus subtilis, and candida utilis, etc, and the selected Chinese Traditional Herbs include astragalus, atractlylis lancea formalyrata, codonopsis pilosula, pericarpium citri reticulatae, and atractylis atractylis ovata, etc. The ferment Chinese Traditional Herbs for feeding provided by the present invention used to breed the livestock and the poultry is capable of preventing and treating epidemic diseases and improving the growth of the animal. The biological ferment of the water extract of Chinese Traditional Herbs by the probiotics is utilized to produce the elements with higher activities, thereby improving the absorption, transportation, metabolism, activation and medical efficacy of the Chinese Traditional Herbs in human body.
Description
One, technical field
The present invention relates to a kind of preparation method of novel ferment Chinese traditional herbs for feeding, belong to the feed addictive production field.
Two, background technology
Along with the development of herding, aquaculture and the raising of people's living standard, people are to the understanding of foodsafety also progressively in-depth, and the cry that requires to produce nuisanceless, safety and green livestock and poultry, aquatic products product is more and more higher.Calendar year 2001, Chinese Government formally issued and implemented " Chinese food and nutrition development outline (2001-2010) ", had highlighted the necessity of food security.Food is the important substance basis that the mankind depend on for existence and development, and the quality safety of food has become one of focus of paying close attention in the whole world.In addition, though the livestock and poultry of China, aquatic products product have bigger price advantage in the international market, because the medicament residue problem, market prospects are also pessimistic.China joins WTO, agricultural product and livestock products will enter the international market and participate in world's competition, for eliminating trade, technology barriers and discrimination, advance safety of China, nuisanceless and green livestock and poultry, aquatic products product to popularize, become the tendency of the day, the important means of invincible position is stood on by domestic especially veterinary drug, production of fodder enterprise in intense market competition.At present, the development and production of no drug residue, safe, nuisanceless and green veterinary drug, feed are praised highly day by day and are paid attention to.But the biggest problem that faces is exactly how to replace the application problem of antibiotic in the livestock and poultry cultivation process.
Along with deepening continuously of animal microecology research, the microorganism fodder additive so that beneficial microbe colony is developed is widely used as a class novel non-pollution, no drug residue feed resource.Because it has BA, can participate in the ecological balance of animal gastrointestinal tract microorganism species well, promote animal digestion to absorb, grow, improve food conversion ratio and production performance, strengthen the cub premunition, and have the unique effect of improving feeding environment and reducing environmental pollution.
The problem that the tradition microorganism fodder additive in use exists:
1. microorganism fodder additive the transportation, use and the preservation process in be easy to inactivation;
2. some bacterial strain enters the effect that can not effectively resist hydrochloric acid, bile acid in the alimentary canal in the microorganism fodder additive, plays a role thereby can't be colonizated in the enteron aisle;
3. the microorganism fodder additive bacterial classification that uses at present is also less, and minority bacterial classifications such as Bacillus acidi lactici, Bifidobacterium, excrement (dung) streptococcus and saccharomycete are only arranged;
4. after active bacteria formulation entered enteron aisle, its speed of growth was slow, was difficult to the status of having the advantage in the microorganism competition, was difficult to form dominant microflora.
The problem that in use exists at microorganism fodder additive, seek new be used for livestock and poultry, aquatic products disease prevention and cure, promote it to digest and assimilate, grow, improve food conversion ratio and production performance, strengthen premunition, reducing dosage, and have the feed addictive that improves feeding environment and reduce the environmental pollution effect, is the difficult problem that present urgent need solves.
Three, summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of preparation method of ferment Chinese traditional herbs for feeding is provided.
Method of the present invention is included in one or more beneficial bacteria of intestinal tract of inoculation of medium that contain Chinese herbal medicine extract, and cultivates it at suitable fermentation condition bottom fermentation.Bacterial classification is one of lactobacillus acidophilus, bacillus subtilis, candida utili bacterium, enterococcus faecalis, Lactobacillus plantarum or saccharomyces cerevisiae or combination.
The preparation method of ferment Chinese traditional herbs for feeding of the present invention, step is as follows:
1, the preparation of Chinese herbal medicine water extract
Selected Chinese herbal medicine was soaked 10-15 hour with drinking water, steam negative pressure extractor extracted 3-4 hour, and pump extracts soup to staticly settling jar, staticly settles 20-30 hour, get the supernatant negative pressure and be concentrated into into soup, liquid in its herb liquid: crude drug weight ratio 1-2: 1-2.Described Chinese herbal medicine is selected from one of following or combination:
The Radix Astragali, the bighead atractylodes rhizome, Radix Codonopsis, dried orange peel, rhizoma atractylodis, hawthorn, the fruit of glossy privet, Radix Angelicae Sinensis, Poria cocos, windproof, Semen Raphani, the bark of eucommia, cordate houttuynia, Radix Glycyrrhizae, Chinese violet, dandelion, selfheal, isatis root, folium isatidis, barrenwort, the red sage root, rascal, the dried immature fruit of citron orange, Chinese yam, ginger, the root of herbaceous peony, the root of bidentate achyranthes, purslane, field pennycress, Psoralea corylifolia, sea-tangle, brown alga, rhizoma cyperi, balloonflower root, radix bupleuri, the root of kudzu vine, rhizome of cyrtomium, motherwort, pine needle, jujube, turmeric, great burdock achene, Asiatic plantain, the capsule of weeping forsythia, Divine Comedy or Fructus Hordei Germinatus.
2, the preparation of fermentation culture medium in the seeding tank
The soup of step 1 preparation is pressed 0.01%-30% liquid medicine weight ratio add in the seeding tank sterilization.
Bacterial classification is one of lactobacillus acidophilus, bacillus subtilis, candida utili bacterium, enterococcus faecalis, Lactobacillus plantarum or brewer's yeast or combination.
The liquid spawn of above-mentioned bacterial classification is inoculated in the seeding tank by the total inoculum concentration of the weight ratio of 1%-10%, be incubated 36-38 ℃, stir fermented and cultured 22-26h;
3, the preparation of fermentation culture medium in the fermentation tank
The soup of step 1 preparation is pressed 0.01%-30% liquid medicine weight ratio add in the fermentation tank sterilization.With fermentation culture medium in step 2 seeding tank, in aseptic pipeline moved into fermentation tank, the 1%-10% that presses fermentation tank inner liquid medicine weight added, and is incubated 36-38 ℃, stirs, and fermented and cultured 46-50 hour, promptly gets the liquid ferment Chinese traditional herbs for feeding then.
Can also carry out the bacterial classification inactivation treatment to the liquid ferment Chinese traditional herbs for feeding of above-mentioned steps 3 gained.Ablation method is by prior art.
The liquid ferment Chinese traditional herbs for feeding of above-mentioned steps 3 gained can also further be prepared into pulvis.The method that is prepared into pulvis is by prior art.
The preferred lactobacillus acidophilus of liquid spawn, bacillus subtilis and candida utili bacterium weight ratio 1-2 in the above-mentioned steps 2: the combination of 1-2: 1-2.
Another preferred version of liquid spawn is lactobacillus acidophilus and candida utili bacterium weight ratio 1-2 in the above-mentioned steps 2: the combination of 1-2.
The preparation of liquid spawn can be adopted prior art in the above-mentioned steps 2.The invention provides following method:
1) preparation of lactobacillus acidophilus liquid spawn
Take out the culture presevation pipe, draw flat board with the MRS solid medium and recover, 37 ℃ of anaerobism are cultivated 48h.In the single colony inoculation 50mlMRS of the picking fluid nutrient medium, 37 ℃ of anaerobism are cultivated 24h in shaking table on plate.Seed adopts 2% inoculum concentration, inoculates rearmounted triangular flask and cultivates 24h in 37 ℃ of anaerobism concussions.
2) preparation of bacillus subtilis liquid spawn
Take out the culture presevation pipe, draw flat board with nutrition nutrient agar culture medium and recover, 37 ℃ of anaerobism are cultivated 48h.On plate, in the single colony inoculation 50ml of the picking nutrition nutrient agar culture medium, in shaking table, cultivate 24h for 37 ℃.Seed adopts 2% inoculum concentration, inoculates rearmounted triangular flask and cultivates 24h in 37 ℃ of concussions.
3) preparation of candida utili bacteria liquid bacterial classification
Take out the culture presevation pipe, after inclined-plane cultivation and seed culture, seed adopts 2% inoculum concentration with the wort agar culture medium, inoculates rearmounted triangular flask and cultivates 24h in 37 ℃ of concussions.
Chinese herbal medicine described in the above-mentioned steps 1 is preferably as follows combination:
The Radix Astragali, the bighead atractylodes rhizome, Radix Codonopsis, dried orange peel, the fruit of glossy privet, Poria cocos, windproof, Radix Glycyrrhizae, barrenwort were by weight 2: 2: 2: 1: 1: 1: 0.5: 0.5: 1.
The Radix Astragali, Radix Codonopsis, dried orange peel, Poria cocos, Radix Glycyrrhizae, barrenwort were by weight 5: 2: 1: 1: 0.5: 1.
The Radix Astragali, isatis root, barrenwort were by weight 4: 4: 2.
The Radix Astragali, Radix Codonopsis, dried orange peel, Poria cocos, windproof, Radix Glycyrrhizae, barrenwort, rhizoma atractylodis, hawthorn, Semen Raphani, sea-tangle, rhizoma cyperi, the capsule of weeping forsythia, Divine Comedy were by weight 2: 1: 1: 1: 0.5: 0.5: 1: 1: 1.5: 0.5: 2: 0.5: 0.5: 1.
The Radix Astragali, Chinese violet, dandelion, ginger, Psoralea corylifolia, rascal, the dried immature fruit of citron orange, the root of herbaceous peony, the root of kudzu vine, Chinese yam, Asiatic plantain, selfheal, Fructus Hordei Germinatus, folium isatidis were by weight 4: 0.5: 0.5: 0.5: 1: 0.5: 0.5: 2: 2: 1.5: 1: 1: 1: 1.
The Radix Astragali, Radix Angelicae Sinensis, rhizome of cyrtomium, the bark of eucommia, cordate houttuynia, field pennycress, balloonflower root, radix bupleuri, the root of bidentate achyranthes, the red sage root, motherwort, jujube, pine needle, turmeric, brown alga, great burdock achene, purslane were by weight 4: 1: 1: 1: 2: 1: 1: 0.5: 0.5: 0.5: 1: 1: 2: 0.5: 1: 0.5: 1.
Chinese herbal medicine described in the above-mentioned steps 1 can singly select the Radix Astragali.
Ferment Chinese traditional herbs for feeding of the present invention is used for the breed of its feeding, aquaculture, various economic animals, and the raising of various pets can prevent and treat eqpidemic disease and promote growth of animal.
Ferment Chinese traditional herbs for feeding of the present invention can make an addition in the feed, also can make an addition to raise in the water.
The present invention selects the one group of three kinds of beneficial bacterium that comprises lactobacillus acidophilus, bacillus subtilis and the candida utili bacterium of having complementary functions and can symbiotic fermentation cultivating for use, three kinds of beneficial bacteriums are seeded in the seeding tank of the fluid nutrient medium that contains one or more Chinese herbal medicine extracts after inclined-plane cultivation and seed culture respectively, and liquid spawn is used in fermented and cultured production.The present invention utilizes probio that the water extract of Chinese herbal medicine is carried out biofermentation, cellulose in the above-mentioned soup, starch, polysaccharide and active constituents of medicine are carried out bio-transformation and structural modification, produce the higher active component of performance, thereby promote the interior absorption of body, transportation, metabolism, activation and the drug action of Chinese herbal medicine.
Ferment Chinese traditional herbs for feeding excellent results of the present invention is as follows:
(1) gives full play to the effect that natural drug is prevented and treated eqpidemic disease and promoted growth of animal.
(2) the collaborative beneficial bacterium that strengthens is bred in animal and bird intestines fast, and by oxygen consumption, advantage competition suppresses the pathogenic bacteria growth, makes the fast quick-recovery normal equilibrium of animal and bird intestines flora.
(3) adsorption site of saturated intestinal mucosa makes the pathogenic bacteria can't field planting on intestinal mucosa.
(4) the collaborative stimulation strengthens activity of immune cells, improves leucocyte and erythrocyte number, strengthens immunity of organisms and premunition.
(5) the collaborative promotion grows fast, saves feed, reduces production costs, and increases economic efficiency.
(6) the collaborative generation that reduces harmful substances such as ammonia, hydrogen sulfide, indoles improves feeding environment.
(7) can obviously improve livestock and poultry muscle and organize local flavor, provide delicious, inexpensive, green, safe food to market.
Four, the specific embodiment
Embodiment 1: the preparation of ferment Chinese traditional herbs for feeding, and the concrete operations step is as follows:
A. the preparation of bacterial classification
1) preparation of lactobacillus acidophilus (ACCC10637) bacterial classification
Take out the culture presevation pipe, draw flat board with the MRS solid medium and recover, 37 ℃ of anaerobism are cultivated 48h.In the single colony inoculation 50mlMRS of the picking fluid nutrient medium, 37 ℃ of anaerobism are cultivated 24h in shaking table on plate.Seed adopts 2% inoculum concentration, inoculates rearmounted triangular flask and cultivates 24h in 37 ℃ of anaerobism concussions.
2) preparation of bacillus subtilis (ACCC11060) bacterial classification
Take out the culture presevation pipe, draw flat board with nutrition nutrient agar culture medium and recover, 37 ℃ of anaerobism are cultivated 48h.On plate, in the single colony inoculation 50ml of the picking nutrition nutrient agar culture medium, in shaking table, cultivate 24h for 37 ℃.Seed adopts 2% inoculum concentration, inoculates rearmounted triangular flask and cultivates 24h in 37 ℃ of concussions.
3) preparation of candida utili bacterium (ACCC20060) bacterial classification
Take out the culture presevation pipe, after inclined-plane cultivation and seed culture, seed adopts 2% inoculum concentration with the wort agar culture medium, inoculates rearmounted triangular flask and cultivates 24h in 37 ℃ of concussions.
B. the fermentation preparation of Chinese herbal medicine water extract
Radix Astragali 20kg, bighead atractylodes rhizome 20kg, Radix Codonopsis 20kg, dried orange peel 10kg, fruit of glossy privet 10kg, Poria cocos 10kg, windproof 5kg, Radix Glycyrrhizae 5kg, barrenwort 10kg are added 12 times of drinking water to be soaked 12 hours, steam negative pressure extractor extracted 3 hours, pump extracts soup to staticly settling jar, staticly settled 24 hours, and got the supernatant negative pressure and be concentrated into 1: 1 (liquid medicine ratio W/W).
C. the preparation of fermentation culture medium in fermentation culture medium and the fermentation tank in the seeding tank
1) ratio of B step gained soup in 10% (liquid medicine ratio W/W) added in seeding tank and the fermentation tank sterilization respectively.
2) with 3 kinds of bacterium of A step gained, 1: 1: 1 by weight percentage proportioning, and be that 5% ratio (W/W) inoculation goes into to have added in the seeding tank of Chinese herbal medicine water extract in total inoculum concentration, be incubated 37 ℃, stirring, fermented and cultured 24h; With fermentation culture medium in the seeding tank, added in the fermentation tank of Chinese herbal medicine water extract through aseptic pipeline immigration then, be incubated 37 ℃, stirred, fermented and cultured promptly got the liquid ferment Chinese traditional herbs for feeding after 48 hours.
Embodiment 2: the preparation of ferment Chinese traditional herbs for feeding, and the concrete operations step is as follows:
A. the preparation of bacterial classification
1) preparation of lactobacillus acidophilus (ACCC10637) bacterial classification
Take out the culture presevation pipe, draw flat board with the MRS solid medium and recover, 37 ℃ of anaerobism are cultivated 48h.In the single colony inoculation 50mlMRS of the picking fluid nutrient medium, 37 ℃ of anaerobism are cultivated 24h in shaking table on plate.Seed adopts 2% inoculum concentration, inoculates rearmounted triangular flask and cultivates 24h in 37 ℃ of anaerobism concussions.
2) preparation of candida utili bacterium (ACCC20060) bacterial classification
Take out the culture presevation pipe, after inclined-plane cultivation and seed culture, seed adopts 2% inoculum concentration with the wort agar culture medium, inoculates rearmounted triangular flask and cultivates 24h in 37 ℃ of concussions.
B. the fermentation preparation of Chinese herbal medicine water extract
Radix Astragali 50kg, Radix Codonopsis 20kg, dried orange peel 10kg, Poria cocos 10kg, Radix Glycyrrhizae 5kg, barrenwort 10kg are added 12 times of drinking water to be soaked 12 hours, steam negative pressure extractor extracted 3 hours, pump extracts soup to staticly settling jar, staticly settled 24 hours, and got the supernatant negative pressure and be concentrated into 1: 1 (liquid medicine ratio W/W).
C. the preparation of fermentation culture medium in fermentation culture medium and the fermentation tank in the seeding tank
1) ratio of B step gained soup in 15% (liquid medicine ratio W/W) added in seeding tank and the fermentation tank sterilization respectively.
2) with 2 kinds of bacterium of A step gained, 1: 1 by weight percentage proportioning, and be that 3% ratio (W/W) inoculation goes into to have added in the seeding tank of Chinese herbal medicine water extract in total inoculum concentration, be incubated 37 ℃, stirring, fermented and cultured 24h; With fermentation culture medium in the seeding tank, added in the fermentation tank of Chinese herbal medicine water extract through aseptic pipeline immigration then, be incubated 37 ℃, stirred, fermented and cultured promptly got the liquid ferment Chinese traditional herbs for feeding after 48 hours.
Embodiment 3: the preparation of ferment Chinese traditional herbs for feeding, and the concrete operations step is as follows:
A. the preparation of bacterial classification is with embodiment 2.
B. the fermentation preparation of Chinese herbal medicine water extract
Radix Astragali 40kg, isatis root 40kg, barrenwort 20kg are added 12 times of drinking water soaked 12 hours, steam negative pressure extractor extracted 3 hours, and pump extracts soup to staticly settling jar, staticly settles 24 hours, gets the supernatant negative pressure and is concentrated into 1: 1 (liquid medicine ratio W/W).
C. the preparation of fermentation culture medium in fermentation culture medium and the fermentation tank in the seeding tank
1) ratio of B step gained soup in 15% (liquid medicine ratio W/W) added in seeding tank and the fermentation tank sterilization respectively.
2) with 2 kinds of bacterium of A step gained, 1: 1 by weight percentage proportioning, and be that 3% ratio (W/W) inoculation goes into to have added in the seeding tank of Chinese herbal medicine water extract in total inoculum concentration, be incubated 37 ℃, stirring, fermented and cultured 24h; With fermentation culture medium in the seeding tank, added in the fermentation tank of Chinese herbal medicine water extract through aseptic pipeline immigration then, be incubated 37 ℃, stirred, fermented and cultured promptly got the liquid ferment Chinese traditional herbs for feeding after 48 hours.
Embodiment 4: the preparation of ferment Chinese traditional herbs for feeding, and the concrete operations step is as follows:
A. the preparation of bacterial classification is with embodiment 2.
B. the fermentation preparation of Chinese herbal medicine water extract
Radix Astragali 200kg is added 12 times of drinking water soaked 12 hours, steam negative pressure extractor extracted 3 hours, and pump extracts soup to staticly settling jar, staticly settles 24 hours, gets the supernatant negative pressure and is concentrated into 1: 1 (liquid medicine ratio W/W).
C. the preparation of fermentation culture medium in fermentation culture medium and the fermentation tank in the seeding tank
1), the ratio of B step gained soup in 10% (liquid medicine ratio W/W) added in seeding tank and the fermentation tank sterilization respectively.
2), with 2 kinds of bacterium of A step gained, 1: 1 by weight percentage proportioning, and be that 5% ratio (W/W) inoculation goes into to have added in the seeding tank of Radix Astragali water extract in total inoculum concentration be incubated 37 ℃, stirring, fermented and cultured 24h; With fermentation culture medium in the seeding tank, added in the fermentation tank of Chinese herbal medicine water extract through aseptic pipeline immigration then, be incubated 37 ℃, stirred, fermented and cultured promptly got the liquid ferment Chinese traditional herbs for feeding after 48 hours.
Embodiment 5: the preparation of ferment Chinese traditional herbs for feeding, and the concrete operations step is as follows:
A. the preparation of bacterial classification is with embodiment 1.
B. the fermentation preparation of Chinese herbal medicine water extract
Radix Astragali 20kg, Radix Codonopsis 10kg, dried orange peel 10kg, Poria cocos 10kg, windproof 5kg, Radix Glycyrrhizae 5kg, barrenwort 10kg rhizoma atractylodis 10kg, hawthorn 15kg, Semen Raphani 5kg, sea-tangle 20kg, rhizoma cyperi 5kg, capsule of weeping forsythia 5kg, Divine Comedy 10kg are added 12 times of drinking water to be soaked 12 hours, steam negative pressure extractor extracted 3 hours, pump extracts soup to staticly settling jar, staticly settled 24 hours, and got the supernatant negative pressure and be concentrated into 1: 1 (liquid medicine ratio W/W).
C. the preparation of fermentation culture medium in fermentation culture medium and the fermentation tank in the seeding tank
1) ratio of B step gained soup in 10% (liquid medicine ratio W/W) added in seeding tank and the fermentation tank sterilization respectively.
2) with 3 kinds of bacterium of A step gained, by weight 1: 1: 1 proportioning, and be that 5% ratio (W/W) inoculation goes into to have added in the seeding tank of Chinese herbal medicine water extract in total inoculum concentration, be incubated 37 ℃, stirring, fermented and cultured 24h; With fermentation culture medium in the seeding tank, added in the fermentation tank of Chinese herbal medicine water extract through aseptic pipeline immigration then, be incubated 37 ℃, stirred, fermented and cultured promptly got the liquid ferment Chinese traditional herbs for feeding after 48 hours.
Embodiment 6: as the preparation method of embodiment 1 described ferment Chinese traditional herbs for feeding, different is bacterial classification is enterococcus, Lactobacillus plantarum and brewer's yeast was by weight 1: 1: 1 combination.
Embodiment 7: applicating example
Present embodiment is intended to occur on the basis of pathological model of ewcastle disease by artificial manufacturing chick, uses ferment Chinese traditional herbs for feeding and carries out immunological regulation, has obtained gratifying effect.For this reason, the little chick with 90 1 age in days health is divided into 3 groups, 30 every group at random.The A group is ferment Chinese traditional herbs for feeding group (product of embodiment 1), and the B group is the Chinese herb feed additive group, and the C group is control group.When 10 ages in days, be that vaccine carries out intramuscular injection with the NDI of 10 times of amounts, to cause the pathological model of new city eqpidemic disease chicken.A group and B group began dispensing in back second day from attacking poison, pressed 1% of forage volume respectively and added medicine, logotype 5 days.The blank group is not added any medicine, raises according to a conventional method.
1, HI antibody titer after each experimental group ND vaccine intramuscular injection the results are shown in Table 1.
The variation of the anti-NDV antibody titer of table 1 different days chicken serum (nlog 2) (X ± S)
| Age in days | A | B | C |
| 17 | 3.170±0.252 | 3.200±0.200 | 2.600±0.100 |
| 28 | 4.300±0.252 | 4.100±0.300 | 3.300±0.300 |
| 35 | 5.200±0.250 | 4.600±0.200 | 4.000±0.300 |
As shown in Table 1, when 17 ages in days, 3 groups of chicken antibody levels be more or less the same (P＞0.05).But when 35 ages in days, A group chicken antibody level is apparently higher than B, C group, significant difference (P＜0.05).Show that day element can strengthen the humoral immune function of chicken.
The immune organ index the results are shown in Table 2.
Each test group chicken bursa exponential sum index and spleen index of table 2
*(X ± S)
| A | B | C | |
| The bursa of farbricius (mg/g) | 6.080±0.012 | 0.580±0.100 | 5.480±0.047 |
| Spleen (mg/g) | 2.760±0.027 | 2.640±0.030 | 2.210±0.033 |
* bursa of farbricius index=bursa of farbricius (mg)/body weight (g); Heavy (the mg)/body weight (g) of index and spleen index=spleen
As shown in Table 2, A group bursa of farbricius index is apparently higher than B group and C group, significant difference (P＜0.05).Index and spleen index is higher than B group and C group.And apparently higher than C group, significant difference (P＜0.05)
2, to the influence of chick body weight: during 47 ages in days, each test group chicken average weight is respectively: A group: 564g, B group: 542g, C group: 526g.As from the foregoing, final body weight A group and B group all are higher than C group, significant difference (P＜0.01)
3, attack recovery situation after the morbidity of malicious chicken: during 10 ages in days, attack all chickens of poison back and all show as situations such as lassitude, diet go down, light slight cough and wheezing.Attack poison back recovery situation and see Table 3 (the recovery standard: the state of mind is good, can stand up, diet desires normal).
Table 3 is attacked chicken morbidity back, poison back recovery situation (only) (percentage)
| Attack poison back fate | A | B | C |
| 3 | 10 (33.3％) | 11 (36.7％) | 3 (10％) |
| 4 | 15 (50％) | 14 (46.7％) | 9 (30％) |
| 5 | 24 (80％) | 22 (73.3％) | 16 (53.3％) |
| 6 | 29 (96.7％) | 28 (93.3％) | 23 (76.7％) |
As shown in Table 4, attack malicious back A group and the recovery of B group chicken soon, and the C group recovers slower.And C organizes in attacking the poison back had 2 chicken deaths in the time of the 5th day, had 3 chickens the nervous symptoms that wryneck is turn-taked to occur in addition, and other two groups of chickens do not have this phenomenon.
The present embodiment evidence, feeding glycolysis Chinese herbal medicine system can strengthen the body hematid immunity function, quickening immune organ grows perfect, can also significantly strengthen NK cytoactive in the chick peripheral blood, participate in complement activation, improve the complement level, strengthen phagocytic function, have tangible immunologic enhancement.Ferment Chinese traditional herbs for feeding has tangible regulating action to the chick that the ewcastle disease symptom occurs in this experiment, compares with traditional decoction, experiment showed, that effect is obvious.
The weight of the bursa of farbricius, spleen, thymus gland can be used to estimate the immune state of chick.The bursa of farbricius is the important central immune organ of bird, and spleen is an immune organ maximum in the body, is the main base that produces antibody.This experimental result shows, the bursa of farbricius exponential sum index and spleen index of ferment Chinese traditional herbs for feeding group chicken all is higher than Chinese herb feed additive group and blank group, show that the infected NDV influence of day maincenter of element set chicken and peripheral immune organ is little, grow normally that the ability that participates in immune response is strong.By the ND antibody horizontal detect is found, than high 1 titre of blank group, and each chicken produces relatively homogeneous of antibody horizontal during ferment Chinese traditional herbs for feeding group chicken 35 ages in days.Show that ferment Chinese traditional herbs for feeding is better than the preceding decoction that ferments to the humoral immunity effect of chick, thereby the explanation ferment Chinese traditional herbs for feeding is being effective aspect the chicken immunity that improves the ewcastle disease disease.
Test shows, symptoms such as spiritual depressed, anorexia occurred attacking the most of chicken in poison back, but ferment Chinese traditional herbs for feeding group and Chinese medicine additive group recovery situation be obviously early than the blank group, and all impassivity symptom and the phenomena of mortality take place.In addition, the recovery quantity of ferment Chinese traditional herbs for feeding group chicken in the time of the 5th day also is higher than the Chinese medicine additive group, illustrate that ferment Chinese traditional herbs for feeding not only can improve immunologic function, the recovery of nervous function is also had certain promotion regulating action, and be better than not preparation by fermentation.
This result of the test finds that the body weight of the chick of ferment Chinese traditional herbs for feeding group and Chinese herb feed additive group shows it when improving immunity of organisms all apparently higher than the blank group, has tangible effect of gain.
Claims (10)
1. the preparation method of ferment Chinese traditional herbs for feeding, step is as follows:
(1) preparation of Chinese herbal medicine water extract
Selected Chinese herbal medicine was soaked 10-15 hour with drinking water, steam negative pressure extractor extracted 3-4 hour, and pump extracts soup to staticly settling jar, staticly settles 20-30 hour, get the supernatant negative pressure and be concentrated into into soup, liquid in its herb liquid: crude drug weight ratio 1-2: 1-2; Described Chinese herbal medicine is selected from one of following or combination:
The Radix Astragali, the bighead atractylodes rhizome, Radix Codonopsis, dried orange peel, rhizoma atractylodis, hawthorn, the fruit of glossy privet, Radix Angelicae Sinensis, Poria cocos, windproof, Semen Raphani, the bark of eucommia, cordate houttuynia, Radix Glycyrrhizae, Chinese violet, dandelion, selfheal, isatis root, folium isatidis, barrenwort, the red sage root, rascal, the dried immature fruit of citron orange, Chinese yam, ginger, the root of herbaceous peony, the root of bidentate achyranthes, purslane, field pennycress, Psoralea corylifolia, sea-tangle, brown alga, rhizoma cyperi, balloonflower root, radix bupleuri, the root of kudzu vine, rhizome of cyrtomium, motherwort, pine needle, jujube, turmeric, great burdock achene, Asiatic plantain, the capsule of weeping forsythia, Divine Comedy or Fructus Hordei Germinatus;
(2) preparation of fermentation culture medium in the seeding tank
The soup of step 1 preparation is pressed 0.01%-30% liquid medicine weight ratio add in the seeding tank sterilization;
Bacterial classification is one of lactobacillus acidophilus, bacillus subtilis, candida utili bacterium, enterococcus faecalis, Lactobacillus plantarum or brewer's yeast or combination;
The liquid spawn of above-mentioned bacterial classification is inoculated in the seeding tank by the total inoculum concentration of the weight ratio of 1%-10%, be incubated 36-38 ℃, stir fermented and cultured 22-26h;
(3) preparation of fermentation culture medium in the fermentation tank
The soup of step (1) preparation is pressed 0.01%-30% liquid medicine weight ratio add in the fermentation tank sterilization; With fermentation culture medium in step (2) seeding tank, in aseptic pipeline moved into fermentation tank, the 1%-10% that presses fermentation tank inner liquid medicine weight added, and is incubated 36-38 ℃, stirs, and fermented and cultured 46-50 hour, promptly gets the liquid ferment Chinese traditional herbs for feeding then.
2. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1 is characterized in that the liquid ferment Chinese traditional herbs for feeding of step (3) gained is also carried out the bacterial classification inactivation treatment.
3. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1 is characterized in that liquid spawn is lactobacillus acidophilus, bacillus subtilis and candida utili bacterium weight ratio 1-2 in the described step (2): the combination of 1-2: 1-2.
4. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1 is characterized in that liquid spawn is lactobacillus acidophilus and candida utili bacterium weight ratio 1-2 in the described step (2): the combination of 1-2.
5. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1, the Chinese herbal medicine that it is characterized in that described step (1) is the combination of following composition:
The Radix Astragali, the bighead atractylodes rhizome, Radix Codonopsis, dried orange peel, the fruit of glossy privet, Poria cocos, windproof, Radix Glycyrrhizae, barrenwort were by weight 2: 2: 2: 1: 1: 1: 0.5: 0.5: 1.
6. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1, the Chinese herbal medicine that it is characterized in that described step (1) is the combination of following composition:
The Radix Astragali, Radix Codonopsis, dried orange peel, Poria cocos, Radix Glycyrrhizae, barrenwort were by weight 5: 2: 1: 1: 0.5: 1.
7. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1, the Chinese herbal medicine that it is characterized in that described step (1) is the combination of following composition:
The Radix Astragali, isatis root, barrenwort were by weight 4: 4: 2.
8. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1, the Chinese herbal medicine that it is characterized in that described step (1) is the combination of following composition:
The Radix Astragali, Radix Codonopsis, dried orange peel, Poria cocos, windproof, Radix Glycyrrhizae, barrenwort, rhizoma atractylodis, hawthorn, Semen Raphani, sea-tangle, rhizoma cyperi, the capsule of weeping forsythia, Divine Comedy were by weight 2: 1: 1: 1: 0.5: 0.5: 1: 1: 1.5: 0.5: 2: 0.5: 0.5: 1.
9. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1, the Chinese herbal medicine that it is characterized in that described step (1) is the combination of following composition:
The Radix Astragali, Chinese violet, dandelion, ginger, Psoralea corylifolia, rascal, the dried immature fruit of citron orange, the root of herbaceous peony, the root of kudzu vine, Chinese yam, Asiatic plantain, selfheal, Fructus Hordei Germinatus, folium isatidis were by weight 4: 0.5: 0.5: 0.5: 1: 0.5: 0.5: 2: 2: 1.5: 1: 1: 1: 1.
10. the preparation method of ferment Chinese traditional herbs for feeding as claimed in claim 1, the Chinese herbal medicine that it is characterized in that described step (1) is the combination of following composition:
The Radix Astragali, Radix Angelicae Sinensis, rhizome of cyrtomium, the bark of eucommia, cordate houttuynia, field pennycress, balloonflower root, radix bupleuri, the root of bidentate achyranthes, the red sage root, motherwort, jujube, pine needle, turmeric, brown alga, great burdock achene, purslane were by weight 4: 1: 1: 1: 2: 1: 1: 0.5: 0.5: 0.5: 1: 1: 2: 0.5: 1: 0.5: 1.
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