CN102894185B - Fermented radix isatidis feed additive and application thereof - Google Patents
Fermented radix isatidis feed additive and application thereof Download PDFInfo
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- CN102894185B CN102894185B CN 201210449873 CN201210449873A CN102894185B CN 102894185 B CN102894185 B CN 102894185B CN 201210449873 CN201210449873 CN 201210449873 CN 201210449873 A CN201210449873 A CN 201210449873A CN 102894185 B CN102894185 B CN 102894185B
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Abstract
The invention discloses a fermented radix isatidis feed additive and an application thereof and relates to a feed additive and an application of the feed additive which is added into a piglet feed as the feed additive for feeding weaned piglets. The feed additive is prepared by the following steps of: mixing lactobacillus acidophilus bacteria powder and streptococcus faecalis bacteria powder according to the mass ratio of (2-4): (6-8), and fermenting radix isatidis powder under the anaerobic condition to release maximum radix isatidis polysaccharides, with the following fermentation conditions: fermentation of a culture medium containing 1-3% of radix isatidis and sucrose, and fermentation process with inoculation amount of 0.2-0.5%, water of 40-50%, the fermentation time of 4-6 days, and the temperature of 35-38 DEG C. The invention utilizes a modern fermentation technology for fermenting the radix isatidis, with the radix isatidis polysaccharides content in the fermented radix isatidis preparation of 20.41-22.30% which is increased by 169-194% compared with raw radix isatidis powder. The invention applies the fermented radix isatidis in the feed for the weaned piglets as the feed additive so as to achieve the effects of improving the production performance and immunity of the piglets.
Description
Technical field
The present invention relates to a kind of feed addictive and application thereof, be specifically related to a kind of Radix Isatidis feed addictive and add the application of feeding weanling pig in the pig starter feed as feed addictive to of fermenting.
Background technology
Radix Isatidis is Chinese traditional Chinese medicine, the successive dynasties book on Chinese herbal medicine is all on the books, dry root for Cruciferae woaded blue platymiscium woaded blue, main product is in the North China Plain and the Huanghe valley of China's North of Yangtze River, by plurality of active ingredients in vivo, antiendotoxin clearing heat and detoxicating, antiviral, antibiotic through too many levels, multipath performance, raising immunity and oxidation resistant effect.
Banlangen Polysaccharide is one of main active ingredient of Radix Isatidis, and antivirus action is arranged, and specificity and nospecific immunity, humoral immunity, cellular immunity are all played certain facilitation, and the bacterial adhesion cell is had inhibitory action.Therefore, the fermentation Radix Isatidis is used for weanling pig as feed addictive, can prevention of diarrhea in piglets and raising weaned piglets and autoimmunity.But, what present stage, Banlangen Polysaccharide adopted basically in preparation technology is water extraction and alcohol precipitation method, this method has not only been wasted most macromolecular polysaccharide constituents, and some water-soluble relatively poor active principle such as alkaloids composition extraction efficiency are lower, thereby curative effect is reduced, become the major technique bottleneck that limits its application.Radix Isatidis is studied few especially aspect fermentation, awaits the researcher and is deeply developing aspect this, enriches the data aspect fermentation.
Generally adopt at present antibiotic, hormone, chemical synthetic drug control grice diarrhoea, the drug resistance of bringing along with the long-term use of these medicines, residual, the toxic and side effect in livestock products and environment etc. day by day serious, serious threat human health.
Summary of the invention
The purpose of this invention is to provide a kind of fermentation Radix Isatidis feed addictive and application thereof, utilize modern fermentation technique that Radix Isatidis is fermented, improve the content of Banlangen Polysaccharide.
The objective of the invention is to be achieved through the following technical solutions:
The present invention mixes with the mass ratio of two kinds of industrial bacterium powder (lactobacillus acidophilus bacterium powder and streptococcus fecalis bacterium powder) with 2 ~ 4:6 ~ 8, and the Radix Isatidis powder of 60 ~ 100 mesh sieves that under anaerobic fermented discharges the Banlangen Polysaccharide maximum, and fermentation condition is as follows:
Fermentation medium: Radix Isatidis, sucrose 1 ~ 3%;
Zymotechnique: inoculum concentration: 0.2 ~ 0.5%, water: 40 ~ 50%, fermentation time: 4 ~ 6 days;
Temperature: 35 ~ 38 ℃.
Oxygen free condition can suppress the growth of other bacteriums effectively, can avoid lactic acid bacteria to be polluted.The sucrose that adds in the fermentation medium is raw material cheap and easy to get, is convenient to actual application.
Use: the Radix Isatidis that will ferment adds the weanling pig of feeding in the pig starter feed as feed addictive to 0.1 ~ 0.5% addition, thereby reaches the raising weaned piglets.
The present invention utilizes modern fermentation technique that Radix Isatidis is fermented, and the Banlangen Polysaccharide content of fermentation isatis root preparation is 20.41 ~ 22.30%, compares with the former powder of Radix Isatidis, has increased by 169 ~ 194%.The present invention selects optimal conditions of fermentation that the Banlangen Polysaccharide maximum is discharged with industrial bacterium powder fermentation Radix Isatidis, and the Radix Isatidis that will ferment is applied to the weanling pig feed as feed addictive, thereby reaches the effect that improves weaned piglets and autoimmunity.
The specific embodiment
Below in conjunction with embodiment the technical solution of the present invention is further explained explanation.
The experimental design of adopting single factor experiment and orthogonal test to combine is optimized factor inoculum concentration, water content, the fermentation time of impact fermentation take final Banlangen Polysaccharide burst size as standard.Wherein the level of inoculum concentration design is 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% in the single factor experiment, and the level of water content design is 20%, 30%, 40%, 50%, 60%, 70%, and the level of fermentation time design is 3d, 5d, 7d, 9d; Orthogonal is the experimental design of Three factors-levels.Every group of test all designs 3 repetitions.The optimal conditions of fermentation of determining the Radix Isatidis powder is: water content is 45%, and inoculum concentration is 0.2%, and fermentation time is 5d, and concrete fermentation step is as follows:
The fermented and cultured that 20 g are comprised of Radix Isatidis, 2% sucrose places 30min under the uviol lamp based in the plastic packaging bag, and is for subsequent use.Lactobacillus acidophilus bacterium powder is mixed with the ratio of 4:6 with streptococcus fecalis bacterium powder, respectively with 0.2%(V/m) inoculum concentration under anaerobic access respectively in the fermentation medium, water content is 45%, with vacuum packing machine the plastic packaging bag is vacuumized plastic packaging, fermentation time is 5 days, is constant temperature culture under 37 ℃ the condition in temperature.60 ℃ of oven dry, measuring the Radix Isatidis polyoses content is 21.47%, compares with the former powder of Radix Isatidis, has increased by 183% after the fermentation.
Add above-mentioned fermentation Radix Isatidis to the weanling pig of feeding in the pig starter feed as feed addictive with 0.30% addition, wherein:
The average daily ingestion amount 543g of control group piglet, average daily gain 253g, feedstuff-meat ratio 2.15, diarrhea rate 0.21%;
The average daily ingestion amount 569g of the former powder group of Radix Isatidis, average daily gain 289g, feedstuff-meat ratio 1.96, diarrhea rate 0.16%;
The average daily ingestion amount 597g of fermentation Radix Isatidis group, average daily gain 324g, feedstuff-meat ratio 1.84, diarrhea rate 0.09%.
This shows that the fermentation isatis root preparation is best to the action effect that improves piglet daily gain, raising efficiency of feed utilization and reduction diarrhea rate.
Immunoglobulin content in the control group piglet blood (ug/ml): IgA 26.32, and IgM 15.27, and IgG 169.94;
Immunoglobulin content (ug/ml) in the former powder group of the Radix Isatidis blood: IgA 27.84, and IgM 15.78, and IgG 170.38;
Immunoglobulin content (ug/ml) in the fermentation Radix Isatidis group blood: IgA 30.49, and IgM 15.99, and IgG 239.06.
Immune factor content (pg/ml) in the control group piglet blood: IL-2 120.11, and IL-4 117.52, TNF-α 87.40;
Immune factor content (pg/ml) in the former powder group of the Radix Isatidis blood: IL-2 111.64, and IL-4 121.77, TNF-α 81.48;
Immune factor content (pg/ml) in the fermentation Radix Isatidis group blood: IL-2 116.36, and IL-4 160.85, TNF-α 80.73;
This shows that the fermentation isatis root preparation has the effect that increases main immunoglobulin and partial immunity cytokine content in the weanling pig blood, thereby has strengthened the resistance against diseases of weanling pig.
Claims (6)
1. the Radix Isatidis feed addictive ferments, it is characterized in that described fermentation Radix Isatidis ferments by the following method forms: lactobacillus acidophilus bacterium powder is mixed with the mass ratio of streptococcus fecalis bacterium powder with 2 ~ 4:6 ~ 8, the Radix Isatidis powder that under anaerobic ferments, fermentation condition is as follows:
Fermentation medium: Radix Isatidis, sucrose 1 ~ 3%;
Zymotechnique: inoculum concentration: 0.2 ~ 0.5%, water: 40 ~ 50%, fermentation time: 4 ~ 6 days; Temperature: 35 ~ 38 ℃.
2. fermentation Radix Isatidis feed addictive according to claim 1 is characterized in that described Radix Isatidis powder crosses 60 ~ 100 mesh sieves.
3. fermentation Radix Isatidis feed addictive according to claim 1 is characterized in that described fermentation condition is:
Fermentation medium: Radix Isatidis, sucrose 2%;
Zymotechnique: inoculum concentration: 0.2%, water: 45%, fermentation time: 5 days; Temperature: 37 ℃.
4. the described fermentation Radix Isatidis of claim 1 feed addictive adds application in the pig starter feed to as feed addictive.
5. the application of fermentation Radix Isatidis feed addictive according to claim 4 is characterized in that described addition is 0.1 ~ 0.5%.
6. the application of fermentation Radix Isatidis feed addictive according to claim 4 is characterized in that described addition is 0.3%.
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103859225B (en) * | 2014-03-21 | 2015-05-20 | 安徽泗县成安牧业科技有限公司 | Pig-immunity-improving feed and preparation method thereof |
| CN105029060A (en) * | 2015-08-07 | 2015-11-11 | 铜陵市康顺牧业有限责任公司 | Feed for weaned pigs and preparing method thereof |
| CN105779337A (en) * | 2016-03-21 | 2016-07-20 | 禹州市天源生物科技有限公司 | Method for comprehensively utilizing radix isatidis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
| CN101658236A (en) * | 2008-08-28 | 2010-03-03 | 湖北正佳微生物工程股份有限公司 | Preparation method of biological feed |
| CN102334600A (en) * | 2011-10-31 | 2012-02-01 | 李耀曾 | Fermented feed and preparation method thereof |
| CN102366021A (en) * | 2011-09-16 | 2012-03-07 | 汪松林 | Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
| CN101658236A (en) * | 2008-08-28 | 2010-03-03 | 湖北正佳微生物工程股份有限公司 | Preparation method of biological feed |
| CN102366021A (en) * | 2011-09-16 | 2012-03-07 | 汪松林 | Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics |
| CN102334600A (en) * | 2011-10-31 | 2012-02-01 | 李耀曾 | Fermented feed and preparation method thereof |
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