CN110982753A - Bacillus coagulans MES847, microbial inoculum, chicken feed, preparation method and application - Google Patents
Bacillus coagulans MES847, microbial inoculum, chicken feed, preparation method and application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/12—Antidiarrhoeals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention provides bacillus coagulans MES847, a microbial inoculum and chicken feed, and a preparation method and application thereof, and relates to the technical field of microorganisms. The Bacillus coagulans MES847 has wide antibacterial spectrum, can be used for preparing products for preventing and/or treating chicken diarrhea, and can improve cellular immunity and humoral immunity, stimulate development of animal immune organs, and improve disease resistance of animals.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a bacillus coagulans MES847, a microbial inoculum, a chicken feed, a preparation method and an application thereof.
Background
In recent years, the phenomenon of water-induced defecation is increased when meat (egg) chickens pull feed, and clinical symptoms are shown as follows: small feed particles which can be digested by stomach are remained in the excrement, and the water content of the excrement is high; the growth speed of the broiler chickens is reduced, the laying rate of the laying hens is reduced, and the feed conversion rate is reduced. Generally, poultry do not have a high mortality rate without secondary disease. The diarrhea and overfeeding symptom has complex etiology, often has no obvious characteristic pathological changes in anatomical examination, is difficult to determine the etiology, and brings certain difficulty to clinical prevention and treatment.
The reason for the formation of the thin material can be summarized into three aspects:
(1) infectious agents
Bacterial enteritis caused by pathogenic escherichia coli has various clinical manifestations, such as dampness of feather below the anus, filth, adhesion, bleeding of intestinal mucosa and congestion, while diarrhea is a sign of bacterial enteritis.
Other diseases such as salmonella infection, bursal disease, renal infectious bronchitis, infectious growth short syndrome, fowl cholera, etc. can also cause diarrhea and overfeeding.
(2) Feed factor
The feed mostly generates mould after mildewing, and mycotoxin is very easy to detect in raw materials such as corn and byproducts thereof, peanut meal, rapeseed meal and the like. The presence of mold and mycotoxins, even if not at levels that severely poison birds, often causes diarrhea and is not effective with antibiotic drugs.
The feed contains anti-nutritional factors, such as trypsin inhibitor in soybean and xylan in wheat, which cause diarrhea of meat (egg) chicken.
When the composition is used for treating diseases, a plurality of antibiotics are taken at the same time, and the long-term taking of the composition easily causes the unbalance of microorganisms in intestinal tracts of meat (egg) chickens, the mass propagation of harmful microorganisms, the obvious decrease of the feed intake of chickens, the diarrhea and the weight gain speed.
In addition, the feed contains too high salt, the anion and cation of the feed are seriously unbalanced, and the feed protein is too high, so that the (meat) laying hens are diluted.
(3) Other factors
In addition to infectious and feed factors, other factors such as coccidiosis and stress factors can cause the (meat) layer chicken to loose.
After the occurrence mechanism of the disease is analyzed, the propagation of the living bacteria in the intestinal tract can play a role in preventing and treating infectious factors and feed reasons.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The main object of the present invention is to provide a bacillus coagulans MES847 to alleviate at least one of the technical problems of the prior art.
The invention provides bacillus coagulans MES847, wherein the bacillus coagulans MES847 is preserved in the China general microbiological culture Collection center in 2018, 8 and 30 months, and the preservation number is CGMCC No. 16358.
The invention also provides application of the bacillus coagulans MES847 in preparation of products for preventing and/or treating chicken diarrhea.
The invention also provides application of the bacillus coagulans MES847 in preparation of products for promoting digestive functions of chickens.
Further, the product is a medicine, a health product or a feed additive.
The invention also provides a microbial inoculum which comprises the bacillus coagulans MES 847;
preferably, the number of effective viable bacteria in the microbial inoculum is more than or equal to 10.0 multiplied by 108Per gram.
The invention also provides application of the microbial inoculum in preparation of products for preventing and/or treating chicken diarrhea.
The invention also provides application of the microbial inoculum in preparation of products for promoting digestive function of chickens.
The invention also provides a preparation method of the microbial inoculum, and the preparation method comprises the steps of sequentially carrying out shake flask culture, primary seed culture, secondary seed culture, fermentation and spray drying on the bacillus coagulans MES847 to obtain the microbial inoculum.
In addition, the invention also provides a chicken feed which comprises a basic chicken feed and the microbial inoculum of the bacillus coagulans MES 847.
Furthermore, 1-2kg of microbial inoculum is added into each ton of basic chicken feed.
The bacillus coagulans MES847 provided by the invention has a wide antibacterial spectrum, has the effects of improving the cellular immunity and humoral immunity levels of an organism, and can stimulate the development of immune organs of animals and improve the disease resistance of the animals. The Bacillus coagulans MES847 can produce protease, lipase, amylase, xylanase, etc., and promote digestion and absorption of nutrients. In addition, the produced vitamins, short-chain fatty acids, amino acids, organic acids, growth promoting factors and the like can provide nutrition for animal organisms, promote the peristalsis of small intestines, improve the digestive function of intestinal tracts and provide favorable conditions for the growth and development of animals.
Furthermore, the bacillus coagulans MES847 provided by the invention has stronger stress resistance, can tolerate simulated gastric acid, simulated bile salt and high-temperature environment, can keep higher survival rate of live bacteria, can reach more than 95 percent, and is more suitable for the requirement of feed processing.
The microbial inoculum provided by the invention comprises bacillus coagulans MES847 provided by the invention. The microbial inoculum is used for preventing and/or treating the diarrhea of the chickens, has remarkable prevention and treatment effect which is over 80 percent, has the characteristics of no phytotoxicity, no residue, no growth inhibition and small dosage, provides possibility for the diversification of medication schemes and the protection of ecological environment, and is convenient for popularization and use. Meanwhile, the microbial inoculum provided by the invention can be added and used in a feed factory in a large scale, and is convenient for farmers to mix the bait.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Unless defined otherwise herein, scientific and technical terms used in connection with the present invention shall have the meanings that are commonly understood by one of ordinary skill in the art. The meaning and scope of a term should be clear, however, in the event of any potential ambiguity, the definition provided herein takes precedence over any dictionary or extrinsic definition. In this application, unless otherwise indicated, the use of the term "including" and other forms is not limiting.
Generally, the nomenclature used, and the techniques thereof, in connection with the cell and tissue culture, molecular biology, immunology, microbiology, genetics and protein and nucleic acid chemistry and hybridization described herein are those well known and commonly employed in the art. Unless otherwise indicated, the methods and techniques of the present invention are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification. Enzymatic reactions and purification techniques are performed according to the manufacturer's instructions, as commonly practiced in the art, or as described herein. The nomenclature used in connection with the analytical chemistry, synthetic organic chemistry, and medical and pharmaceutical chemistry described herein, and the laboratory procedures and techniques thereof, are those well known and commonly employed in the art.
The strain preservation date of the invention is 2018, 8 months and 30 days, and the preservation number is as follows: CGMCC No. 16358. The classification is named as: bacillus coagulans (Bacillus coagulans) with the preservation unit name of China general microbiological culture Collection center (CGMCC for short), No. 3 Xilu-Beijing university Tokyo-Yang district No.1, China academy of sciences; the zip code 100101.
Bacillus coagulans is a facultative anaerobic gram-positive bacterium, widely existing in nature. The bacillus coagulans is in a long rod shape, two ends of the bacillus coagulans are blunt and round, no flagellum exists, and spores are grown; the colony is opaque white, round and prominent in surface, and grows well at 15-40 ℃, and the optimal pH value of the growth is 6.6-7.0. It also belongs to intestinal lactic acid bacteria because it can produce a large amount of lactic acid after fermentation. The bacillus coagulans has the functions of maintaining intestinal microecological balance of common lactic acid bacteria and improving the digestion function and health level of organisms, has the advantages of high temperature resistance, acid resistance, cholate resistance and the like, can effectively inhibit the growth of harmful bacteria, improves the intestinal microecological environment, promotes the intestinal development and enhances the intestinal function, has the characteristics of easy preservation, high reactivation rate and the like, and has good application prospect in the feed industry.
The bacillus coagulans MES847 provided by the invention is separated from soil in Baoding city of Hebei province by 5-month-my-company research and development personnel in 2018.
Based on the characteristics of the bacillus coagulans MES847, the invention also provides the application of the bacillus coagulans MES847 in preparing products for preventing and/or treating chicken diarrhea and products for promoting chicken digestion.
The bacillus coagulans MES847 provided by the invention has a wide antibacterial spectrum, has the effects of improving the cellular immunity and humoral immunity levels of organisms, and can stimulate the development of immune organs of animals and improve the disease resistance of the animals. The Bacillus coagulans MES847 can produce protease, lipase, amylase, xylanase, etc., and promote digestion and absorption of nutrients. In addition, the produced vitamins, short-chain fatty acids, amino acids, organic acids, growth promoting factors and the like can provide nutrition for animal organisms, promote the peristalsis of small intestines, improve the digestive function of intestinal tracts and provide favorable conditions for the growth and development of animals.
In some preferred embodiments, the product is an oral product, further can be a drug, nutraceutical, or feed additive.
The bacillus coagulans MES847 provided by the invention has stronger stress resistance, can tolerate simulated gastric acid, simulated bile salt and high-temperature environment, can keep higher survival rate of viable bacteria, can reach more than 95 percent, is convenient to take orally, and is more suitable for the requirements of medicine, health-care products or feed processing.
According to one aspect of the invention, the invention also provides a microbial inoculum, which comprises the bacillus coagulans MES 847.
The microbial inoculum can take bacillus coagulans MES847 as a main active ingredient; bacillus coagulans MES847 can also be used as auxiliary active ingredient in combination with other microorganisms or functional ingredients. Since the microbial inoculum contains the bacillus coagulans MES847, the microbial inoculum has all the beneficial effects of bacillus coagulans MES 847. The microbial inoculum is used for preventing and/or treating the diarrhea of the chickens, has remarkable prevention and treatment effect which is over 80 percent, has the characteristics of no phytotoxicity, no residue, no growth inhibition and small dosage, provides possibility for the diversification of medication schemes and the protection of ecological environment, and is convenient for popularization and use. Meanwhile, the microbial inoculum provided by the invention can be added and used in a feed factory in a large scale, and is convenient for farmers to mix the bait.
Preferably, the number of effective viable bacteria in the microbial inoculum is more than or equal to 10.0 multiplied by 108Per gram.
According to another aspect of the invention, the application of the microbial inoculum in preparing products for preventing and/or treating chicken diarrhea and the application in preparing products for promoting the digestive function of chicken are also provided.
The broad antibacterial spectrum of the active ingredient bacillus coagulans MES847 in the microbial inoculum is utilized to play an effective antagonistic role on pathogenic bacteria of the chicken diarrhea, and the microbial inoculum containing the bacillus coagulans can play a good role in preventing and/or treating the chicken diarrhea.
The invention also provides a preparation method of the microbial inoculum, and the preparation method comprises the steps of sequentially carrying out shake flask culture, primary seed culture, secondary seed culture, fermentation and spray drying on the bacillus coagulans MES847 to obtain the microbial inoculum.
The preparation method of the microbial inoculum provided by the invention has the advantages of simple process and convenient operation, and is suitable for large-scale amplification culture.
Specifically, the preparation method of the microbial inoculum comprises the following steps:
① shaking culture, wherein the shaking culture medium is MRS culture medium (10 g of beef protein powder, 10g of fish juice, 5g of yeast extract powder, 20g of glucose, 5g of sodium acetate, 2g of diammonium citrate, 800.1 ml of tween, 0.58g of magnesium sulfate, 0.28g of manganese sulfate, 1000ml of distilled water and pH value adjustment of 6.2-6.6), and the culture conditions are that the rotation speed is 200 and the temperature is 230r/min, and the culture is carried out for 30-36 h.
② first-stage seed culture, wherein 100ml of first-stage seed culture medium is filled in a 500ml eggplant bottle, moist heat sterilization is carried out for 20-35min at the temperature of 116-121 ℃, 10ml of bacillus coagulans live bacteria freeze-drying tube strain is inoculated in the first-stage seed culture medium, and culture is carried out for 10-12h at the temperature of 30-37 ℃, and the raw materials and the dosage of the first-stage seed culture medium are 1 percent of beef protein powder, 1 percent of fish juice, 0.5 percent of yeast extract powder, 2 percent of glucose, 0.5 percent of sodium acetate, 0.2 percent of diammonium citrate, 800.01 percent of tween, 0.058 percent of magnesium sulfate, 0.028 percent of manganese sulfate and the pH value of the culture medium is 6.2-6.6;
③ Secondary seed culture, comprising placing 150L secondary seed culture medium in 300L fermentation tank, sterilizing at 115-122 deg.C for 20-40min, inoculating 300ml primary seed into the secondary seed culture medium, culturing at 30-38 deg.C for 10-15h, wherein the culture medium for secondary seed culture comprises peptone 1%, yeast extract 0.25%, glucose 1.0%, sucrose 1.0%, and K2HPO40.4％、MnSO4·H2O0.02% and MgSO4·7H20.05 percent of O and 6.8 +/-0.2 of pH value;
④ fermentation at 5m3Or 30m3The enlarged fermentation tank contains 4000L or 20m of3Sterilizing the bacillus coagulans living bacteria fermentation culture medium at 15-122 ℃ for 20-40min, inoculating 150L or 200L of secondary seeds into the bacillus coagulans living bacteria fermentation culture medium, controlling the tank pressure to be 0.02-0.07 Mpa, and culturing at 30-38 ℃ for 36-48 h; the raw materials of the culture medium used for fermentation comprise 1% of peptone, 0.25% of yeast extract, 1.0% of glucose, 1.0% of sucrose and K2HPO40.4％、MnSO4·H2O0.02% and MgSO4·7H20.05 percent of O and 6.8 +/-0.2 of pH value;
⑤ ending the fermentation when the number of the bacillus coagulans reaches the fermentation level;
⑥ spray drying the fermentation liquid to form powder:
⑦ the effective viable count of Bacillus coagulans product is not less than 10.0 × 108Per gram.
⑧ treating the tail gas by chemical oxidation, plasma irradiation or ultraviolet irradiation to remove odor, remove ammonia nitrogen or remove hydrogen sulfide, and discharging.
In addition, the invention also provides a chicken feed which comprises a basic chicken feed and the bacillus coagulans MES847 microbial inoculum.
Since the chicken feed contained bacillus coagulans MES847 as described above, it had all the beneficial effects of bacillus coagulans MES 847. The chicken feed disclosed by the invention is used for preventing and/or treating chicken diarrhea, has an obvious prevention and treatment effect, is more than 80% in prevention and treatment effect, is pollution-free and pollution-free, is low in cost, is suitable for popularization and use, and is convenient for farmers to mix with the feed.
It should be noted that the type of the basic chicken feed is not limited, and all chicken feeds which are conventionally available in the field can be used for preparing the chicken feed provided by the invention.
In some preferred embodiments, 1-2kg of inoculant is included per ton of basal chicken feed, and may for example include 1kg, 1.2kg, 1.5kg, 1.8kg or 2kg of inoculant.
According to the treatment or prevention effect, the microbial inoculum with different dosages can be selectively added. For example, in prophylactic applications, relatively low doses may be administered chronically at relatively low frequency intervals, and in therapeutic applications, it is sometimes desirable to administer relatively high doses at relatively short intervals, which may be adjusted within reasonable ranges according to actual requirements.
The technical solution and the advantages of the present invention will be further explained with reference to the preferred embodiments.
EXAMPLE 1 isolation and screening of the strains
The strain of bacillus coagulans MES847 is separated from soil in Baoding city of Hebei province by 5-month-I-M-company researchers in 2018. Then 3 lactic acid bacteria were co-isolated, streaked, cultured at 32 ℃ for 36 hours to prepare slant strains, and refrigerated at 4 ℃. Selecting single bacterial colony, slicing, crystal violet staining, microscopic examination and physiological and biochemical test to finally determine that one bacterial colony is bacillus coagulans, and the bacillus coagulans is named as MES 847.
Example 2 preparation of Bacillus coagulans MES847 preparation
The method comprises the following steps: shake flask culture, primary seed culture, secondary seed culture, fermentation, spray drying, etc. Specifically, the method comprises the following steps:
① shaking culture, wherein the shaking culture medium is MRS culture medium (10 g of beef protein powder, 10g of fish juice, 5g of yeast extract powder, 20g of glucose, 5g of sodium acetate, 2g of diammonium citrate, 800.1 ml of tween, 0.58g of magnesium sulfate, 0.28g of manganese sulfate, 1000ml of distilled water and pH value adjustment of 6.2-6.6), and the culture conditions are that the rotation speed is 200 and the temperature is 230r/min, and the culture is carried out for 30-36 h.
② first-stage seed culture, wherein 100ml of first-stage seed culture medium is filled in a 500ml eggplant bottle, moist heat sterilization is carried out for 20-35min at the temperature of 116-121 ℃, 10ml of bacillus coagulans live bacteria freeze-drying tube strain is inoculated in the first-stage seed culture medium, and culture is carried out for 10-12h at the temperature of 30-37 ℃, and the raw materials and the dosage of the first-stage seed culture medium are 1 percent of beef protein powder, 1 percent of fish juice, 0.5 percent of yeast extract powder, 2 percent of glucose, 0.5 percent of sodium acetate, 0.2 percent of diammonium citrate, 800.01 percent of tween, 0.058 percent of magnesium sulfate, 0.028 percent of manganese sulfate and the pH value of the culture medium is 6.2-6.6;
③ Secondary seed culture, comprising placing 150L secondary seed culture medium in 300L fermentation tank, sterilizing at 115-122 deg.C for 20-40min, inoculating 300ml primary seed into the secondary seed culture medium, culturing at 30-38 deg.C for 10-15h, wherein the culture medium for secondary seed culture comprises peptone 1%, yeast extract 0.25%, glucose 1.0%, sucrose 1.0%, and K2HPO40.4％、MnSO4·H2O0.02% and MgSO4·7H20.05 percent of O and 6.8 +/-0.2 of pH value;
④ fermentation at 5m3Or 30m3The expansion fermentation tank is filled with 4000L or 20m3Sterilizing the live bacillus coagulans fermentation medium at 15-122 deg.C for 20-40min, and mixing 150L or 200LInoculating the grade seed into a bacillus coagulans viable bacteria fermentation culture medium, controlling the pressure of the tank to be 0.02Mpa-0.07Mpa, and culturing at 30-38 ℃ for 36-48 h; the raw materials of the culture medium used for fermentation comprise 1% of peptone, 0.25% of yeast extract, 1.0% of glucose, 1.0% of sucrose and K2HPO40.4％、MnSO4·H2O0.02% and MgSO4·7H20.05 percent of O and 6.8 +/-0.2 of pH value;
⑤ ending the fermentation when the number of the bacillus coagulans reaches the fermentation level;
⑥ spray drying the above fermentation liquid to get powder:
⑦ the effective viable count of Bacillus coagulans product is not less than 10.0 × 108Per gram.
⑧ treating the tail gas by chemical oxidation, plasma irradiation or ultraviolet irradiation to remove odor, remove ammonia nitrogen or remove hydrogen sulfide, and discharging.
Example 3 test of therapeutic Effect of Bacillus coagulans
(1) Time and place of experiment:
the experiment was carried out in two broiler breeding greenhouses of the double-pond poplars scholar village in the quiet sea area of Tianjin in 2019, 6 months and 8 days.
(2) Testing greenhouse conditions:
the cultivation greenhouse is a simple east-west greenhouse, the south side and the north side are covered by thick plastic, keel supports are arranged in the cultivation greenhouse, and adobe walls of entrances and exits are arranged on the two sides of the east and west. The greenhouse is 60 meters from east to west; the span between south and north is 9.5 meters, the height of the middle is about 2 meters, the height of both south and north is about 1.5 meters, the breeding mode is net breeding, and the breeding variety is broiler chicken. Before the test, the situation of diarrhea occurs to different degrees in the broilers cultured in 2 greenhouses.
(3) The test method comprises the following steps:
the No.1 is a control greenhouse and has light diarrhea symptoms, and the No. 2 is a test greenhouse and has heavy symptoms; the broiler chickens in the No. 2 greenhouse are mixed with the bacillus coagulans preparation prepared in the example 2 at the beginning of the test, and the dosage of the bacillus coagulans preparation is 200g preparation per 100kg feed, namely 2kg preparation per ton feed. One treatment course is 7 days continuously, and two treatment courses are continuously used; no.1 greenhouse broiler chickens do not take any products.
After 7 days (6 months and 15 days) in the No. 2 greenhouse, the symptoms of diarrhea of the broilers are relieved, and then the phenomenon of diarrhea completely disappears after the broilers are continuously mixed for one treatment period to 6 months and 22 days, so that the broilers eat normally. And the symptoms of the 2# greenhouse diarrhea are aggravated, the number is increased, and the appetite is reduced.
Example 4 preventive effect test of Bacillus coagulans preparation
(1) Time and place of experiment: the experiment was carried out in three broiler breeding greenhouses of the double-pond populus scholartree village in the quiet sea area of Tianjin from 7/6 th in 2019, and 3 greenhouses are all chicks bred for 10 days.
(2) Testing greenhouse conditions:
the 3 greenhouses are all broiler breeding greenhouses and are respectively marked as 3#, 4#, and 5 #. The three chicken houses are used for putting seedlings, feeding, adding water and the like, and the conditions are kept consistent. Three greenhouses were fed normally before the start of the test, without "diarrhea".
(3) The test method comprises the following steps:
randomly selecting a 3# greenhouse as a test greenhouse, and selecting a 4# greenhouse and a 5# greenhouse as comparison greenhouses. In a test greenhouse, the bacillus coagulans preparation prepared in the example 2 is continuously stirred, and the using dosage is 100-200g of microbial inoculum per 100kg of feed. Namely 1kg-2kg of microbial inoculum is used for each ton of feed. And directly feeding the broiler chickens in the control greenhouse with the feed which is not mixed with the bacillus coagulans preparation.
In 20 days and 23 days after 7 months, the greenhouse of the control group generates the symptoms of diarrhea in sequence; the test greenhouse has no 'diarrhea' symptom until the chickens are sold. The utilization rate of the greenhouse feed of the control group is obviously lower than that of the experimental group.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. The bacillus coagulans MES847 is characterized in that the bacillus coagulans MES847 is preserved in the China general microbiological culture Collection center (CGMCC) in 2018, 8 and 30, with the preservation number of CGMCC No. 16358.
2. Use of bacillus coagulans MES847 as claimed in claim 1 for the preparation of a product for the prevention and/or treatment of chicken diarrhea.
3. Use of bacillus coagulans MES847 as claimed in claim 1 for the preparation of a product for promoting digestive function in chickens.
4. Use according to claim 2 or 3, wherein the product is a pharmaceutical, nutraceutical or feed additive.
5. A microbial preparation comprising Bacillus coagulans MES847 according to claim 1;
preferably, the number of effective viable bacteria in the microbial inoculum is more than or equal to 10.0 multiplied by 108Per gram.
6. Use of the bacterial agent of claim 5 in the preparation of a product for preventing and/or treating chicken diarrhea.
7. The use of the bacterial agent of claim 5 in the preparation of a product for promoting digestive function of chicken.
8. The preparation method of the microbial inoculum according to claim 5, which comprises the steps of sequentially carrying out shake flask culture, primary seed culture, secondary seed culture, fermentation and spray drying on bacillus coagulans MES847 to obtain the microbial inoculum.
9. A chicken feed comprising a basal chicken feed and the Bacillus coagulans MES847 of claim 1 or the microbial agent of claim 5.
10. The chicken feed of claim 9, wherein 1-2kg of microbial inoculum is added per ton of basal chicken feed.
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| CN113151066A (en) * | 2021-03-31 | 2021-07-23 | 长沙和光生物科技有限公司 | Bacillus coagulans HALO178 and preparation method thereof |
| CN114891690A (en) * | 2022-06-08 | 2022-08-12 | 山东仙普爱瑞科技股份有限公司 | Complex microbial inoculant with growth promoting performance and application thereof |
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