CN104206789A - Feed additive for preventing cow mastitis and brucellosis and preparation method thereof - Google Patents
Feed additive for preventing cow mastitis and brucellosis and preparation method thereof Download PDFInfo
- Publication number
- CN104206789A CN104206789A CN201410227089.5A CN201410227089A CN104206789A CN 104206789 A CN104206789 A CN 104206789A CN 201410227089 A CN201410227089 A CN 201410227089A CN 104206789 A CN104206789 A CN 104206789A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- brucellosis
- mastitis
- preventing
- feed addictive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a feed additive for preventing cow mastitis and brucellosis and belongs to the technical field of feed. The feed additive comprises, by weight, 35-60% of yeast culture, 1-10% of cecropin, 8-15% of wolfberry, 8-15% of Chinese-date, 5-10% of safflower, 5-10% of white peony root and 4-8% of montmorillonite. The feed additive can effectively prevent cow mastitis and brucellosis, improve cow health conditions, and prevent influence produced by cow mastitis on a milk yield and milk quality. The feed additive greatly improve cow culture economic benefits and avoids unfavorable influence produced by antibiotic on milk quality and cow health.
Description
Technical field
The invention belongs to field of feed, being specifically related to a kind of for preventing feed addictive of mastitis for milk cows and brucellosis and preparation method thereof.
Background technology
Mammitis, particularly recessive mastitis is a kind of breast disease that the Lactation of Dairy Cow phase incidence of disease is very high, can make trouble bovine lactation technical ability and give milk sharply to decline, and causes milk cow Reproductive Performance to lack of proper care, become common disease and the frequently-occurring disease of vast cattle farm, seriously govern the development of dairy.According to statistics, there is a mammitis in milk cow, the output of milk on average declines 8%, and the lighter declines 5%, weighs to reach 26%-10%.The incidence of disease of China's mastitis for milk cows is very high, and the survey showed that for Yuan Yonglong etc., and clinical mammitis incidence of disease average out to 33.41%, recessive mastitis average positive rate is 73.91%.
Brucellosis (also claiming brucellosis, " cloth is sick ") is the zoonosis caused by brucella (also claiming Brucella), be distributed in all over the world widely, also there is popular and distribution in China, be classified as two class animal epidemics, brucellosis is the miscarriage caused is one of topmost reason in milk cow miscarriage, brings serious harm to animal husbandry and human health.The most significant symptom of brucellosis is that dam is miscarried, and the drove miscarriage incidence of disease of neopathy is higher, and the old epidemic-stricken area miscarriage incidence of disease is lower, but mammitis, endometritis and the Repeat breeding incidence of disease are higher.Cloth disease worldwide, in endemic, be neglectful in taking precautions by some cattle farms, thinks little of on-the-spot quarantine and isolation system when introducing a fine variety, and brings very large threat to cows health.Monitoring result shows, by the abortion ratio of cloth sick infected zone milk cow up to 12.1%, the miscarriage caused due to cloth disease accounts for 57.1% of abortion number.
Current antibiotic is the choice drug for the treatment of mammitis and brucellosis, but Long-Time Service antibiotic brings a lot of harm, and as antibody-resistant bacterium increases, endocrine dysfunction, antibiotic residue and residue of veterinary drug exceed standard, and badly influence the health of the mankind.At present for mammitis and brucellosis, be main mainly with treatment, with breeding, important impact created to the lactation of milk cow when this disease occurs, seldom seeing the feed addictive for preventing mastitis for milk cows and brucellosis.
Yeast culture fully to be fermented on specific culture medium a kind of Tiny ecosystem goods obtained by saccharomycete under specific process conditions, formed primarily of yeast metabolism product, variation culture medium and active dry yeasr cell.Yeast culture contains peptide class, amino acid, vitamin, enzyme and many to promoting the materials such as useful " UGFs " of growth of animals or poultry.Yeast culture partly can replace antibiotic, and yeast metabolism product antibacterial peptide has broad spectrum antibiotic activity, has very strong lethal effect to bacterium; therefore, yeast culture, as feed addictive, can improve milk cow immunity of organisms; control Infected with Brucella, effectively prevention cloth is sick.Other compositions of yeast culture, can strengthen the lumen fermentation function of milk cow, improve cow feeding amount, for milk cow provides abundant nutriment, can also play the effect improving dairy products quality.
Strengthened the immunity function of animal by the use of Chinese herbal medicine, the incidence of disease of reduction animal becomes study hotspot in recent years.Chinese herbal medicine, as the medicine of disease prevention and cure or feed addictive, is particularly useful for the needs that current milk cow nonreactiveization is produced.Chinese herbal medicine has residual few, the advantages such as pathogenic bacteria not easily develop immunity to drugs, its effect not only has directly suppression and killing action for microorganism itself of curing the disease, the more important thing is and object-immunity system of feeding is adjusted, improve immunity function and the anti-stress ability of feeding animals, medicinal herb components is made into feed addictive, meets development non-nuisance completely for preventing mastitis for milk cows and brucellosis, producing the disease control criterion of safe animal products.
Summary of the invention
For the above-mentioned problems in the prior art, the present invention aims to provide a kind of for preventing the technical scheme of the feed addictive of mastitis for milk cows and brucellosis and preparation method thereof.This feed addictive comprises yeast culture and multiple Chinese herbal medicine is primary raw material, through repeatedly testing the proportion relation determined between each component, to mammitis and brucellosis, there is good preventive effect, and selected raw material is natural harmless, can not have an impact to dairy food quality and health.
Described is a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis, it is characterized in that being made up of the raw material of following percentage by weight:
Yeast culture 35-60%, cecropin 1-10%, fruit of Chinese wolfberry 8-15%, date 8-15%, safflower 5-10%, root of herbaceous peony 5-10% and montmorillonite 4-8%.
Described is a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis, it is characterized in that being made up of the raw material of following percentage by weight:
Yeast culture 45-55%, cecropin 2-8%, fruit of Chinese wolfberry 10-13%, date 10-13%, safflower 6-8%, root of herbaceous peony 6-8% and montmorillonite 5-7%.
Described is a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis, it is characterized in that being made up of the raw material of following percentage by weight:
Yeast culture 48-52%, cecropin 4-6%, fruit of Chinese wolfberry 10-12%, date 10-12%, safflower 7-8%, root of herbaceous peony 7-8% and montmorillonite 6-7%.
Described is a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis, it is characterized in that described yeast culture is candida utili culture, brewer's yeast culture or rhodotorula culture.
Described is a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis, it is characterized in that described yeast culture is obtained by following steps:
1) seed liquor preparation: by candida utili (
candida utilis) CGMCC NO.7691, brewer's yeast or rhodotorula be seeded in liquid seed culture medium, obtain seed liquor, described liquid seed culture medium contains: molasses 10-20g/L, peptone 10-20g/L, sodium chloride 5-25g/L, pH 5.5-7.0, seed culture condition is: shaking table is cultivated, rotating speed 150-200r/min, temperature 28-32 DEG C, incubation time 18-24h;
2) liquid aerobic fermentation: seed liquor is seeded in the liquid fermentation medium of fermentation tank, amplify and cultivate, carry out aerobic fermentation, obtain liquid spawn solution, described liquid fermentation medium contains: molasses 40-80g/L, peptone 30-50g/L, sodium chloride 5-25 g/L, MgSO47H2O 1-5 g/L, pH 5.5-7.0, liquid aerobic fermentation condition is: inoculum concentration 10-20%, throughput 1-3L/min, speed of agitator 200-400r/min, fermentation tank pH automatic feedback regulating system is utilized to control zymotic fluid pH 5.5-7.0, remaining sugar concentration controls at 4-8wt%, fermentation temperature 25-35 DEG C, fermentation time 20-24h,
3) solid aerobic fermentation: by liquid spawn solution inoculum on sterilized solid medium, stone is in fermentation bed, carry out solid aerobic fermentation, described fermentation bed comprises the feeding device connected successively, installation for fermenting and blanking device, described installation for fermenting comprises 2-5 the chain-belt type fermentation interlayer be dislocatedly distributed from top to bottom, described solid medium contains: corn flour 45-65%, wheat bran 15-25%, dregs of beans 20-35%, solid aerobic fermentation condition: fermentation bed stone moisture 40-60%, inoculum concentration 10-20%, the fermentation temperature of each fermentation interlayer is 25-45 DEG C, throughput is 0.1-10 L/min, stirring is controlled at 3-6 time between yeast phase, aerobic fermentation time controling is at 24-36h,
4) solid anaerobic fermentation: control fermentation bed, close ventilation switch, carry out solid anaerobic fermentation, obtain solid fermentation product, solid anaerobic fermentation condition: the fermentation temperature of each fermentation interlayer is 25-45 DEG C, stuffiness, control stirring between yeast phase at 1-3 time, anaerobic fermentation time controling is at 12-18h;
5) solid fermentation product be dry, pulverize 40-60 order, obtain yeast culture.
Described is a kind of for preventing the preparation method of the feed addictive of mastitis for milk cows and brucellosis, it is characterized in that comprising following processing step:
(1) raw material is taken in proportion;
(2) the raw material mixing will taken, about being crushed to about 40-60 order, mixes thoroughly and obtains of the present invention for preventing the feed addictive of mastitis for milk cows and brucellosis.
Candida utili in the present invention (
candida utilis), depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, No. 1, BeiChen West Road, Chaoyang District, BeiJing City institute, preservation day: on 06 09th, 2013, preserving number: CGMCCNo.7691.
Of the present invention for preventing the application of the feed addictive of mastitis for milk cows and brucellosis in Cow-feeding, in the formula of the milk cow mixed feed of routine, add the of the present invention for preventing the feed addictive of mastitis for milk cows and brucellosis of 0.5-1.0wt%.
Of the present invention for preventing the feed addictive of mastitis for milk cows and brucellosis to have the following advantages and beneficial effect:
Feed addictive of the present invention has mainly selected natural, safe yeast culture and medicinal herb components.
Yeast culture is that the product Yikang that Zhejiang Kefeng Biotechnology Co., Ltd produces is precious, this product utilization produces the fermentation of Ruan's Candida degree of depth and forms, formed through aerobic fermentation and anaerobic fermentation by distinctive solid-liquid combined ferment technique and the automatic fermenting organism reactor of chain-belt type with independent intellectual property right, containing abundant antibacterial peptide, active yeast and other yeast metabolism products.Antibacterial peptide rich content in this yeast culture, antibacterial Toplink penetrates bacterial cell membrane and kill bacteria and drug resistance is very low, effectively can improve animal immunizing power, prevention mastitis for milk cows and cloth sick.In Yikang treasured, active dry yeasr bacterial content reaches 10,000,000,000/g, beta glucan content reaches 5%, can significantly improve milk cattle rumen fermentation function and immunity of organisms, other yeast metabolism things are as vitamin, amino acid etc. all can provide nutrition to milk cow, improve milk cow health situation, and mouthfeel and the smell of feed can be improved, there is certain food calling effect.
Cecropin is common commercially available prod, cecropin antimicrobial peptides sterilization mechanism is unique, pathogen not easily produces drug resistance to antibacterial peptide, antibacterial peptide product has broad-spectrum antibacterial action, livestock and poultry are had to the function of growth promotion, health care and disease therapy, belong to have no side effect, noresidue, without the class environment-friendly type preparation causing bacterial drug resistance.Bacteriostatic test shows that antibacterial peptide has very strong lethal effect to 12 kinds of germs such as livestock and poultry Main Pathogenic Bacteria Salmonella, Escherichia coli, enterococcus faecalis, streptococcus fecalis, golden yellow glucose coccuses, but does not injure the beneficial bacterium such as Bacillus acidi lactici, Bifidobacterium.Antibacterial peptide also can tolerate high temperature during feed granulating, and active maintenance well, is applicable to feed addictive of the present invention.
Follow the equipping rules of " monarch ", determine kind and the optimum amount of Chinese herbal medicine in feed addictive through test, make Synergistic between various traditional Chinese medicine ingredients, mutually promote, effectively improve animal body immunity, wherein
Matrimony vine, has effect of nourshing kidney moistening lung, for asthenia of essence and blood of suffering from a deficiency of the kidney; Date, has tonifying Qi, effect of moistening lung toxin expelling; Safflower, has another name called safflower, effect that having invigorates blood circulation leads to breast, the stasis of blood of dispelling stops ulcer, and its external application is inhibited for staphylococcus aureus, beta hemolytic streptococcus, uses and also have certain antivirus action when high concentration; The root of herbaceous peony, has the effect of the astringe yin that nourishes blood, also has antibacterial action, to various bacteria and fungal infection effective; Montmorillonite, is the layer mineral be made up of the hydrous alumino silicates that particle is superfine, has good suction-operated to Escherichia coli, comma bacillus, campylobacter jejuni, staphylococcus aureus and rotavirus and cholate; Fixation is have to bacterial poison; Montmorillonite can provide various trace elements, for animal provides lot of trace composition, can also extend the time of staying of feed at enteron aisle, thus improves the price of deed.Date wherein used has certain sugariness, can make up the impact of other traditional Chinese medicine ingredients on feed local flavor, and with safflower, the root of herbaceous peony with the use of, reach good synergy, improve antibacterial effect.Add montmorillonite and there is good suction-operated, can increase feed addictive stomach carries the high stream time on the one hand, make it maximize to be absorbed, on the other hand can the toxin that produces of mycotoxin effectively in adsorption feed and enteric microorganism metabolism, the premunition of effective raising milk cow, also toxin metabolism can be avoided to enter emulsion, reach good synergistic function with other compositions.
Adopt feed addictive of the present invention can effectively prevent mastitis for milk cows and brucellosis, improve the health status of milk cow, avoid because the generation of the illnesss such as mammitis is on the impact of Milk Production and dairy food quality.Adopt feed addictive of the present invention, drastically increase the economic benefit of milk cattle cultivating, and the adverse effect that antibiotic usage produces dairy food quality and milk cow health can be avoided.
Detailed description of the invention
The present invention is further illustrated below in conjunction with embodiment.
Embodiment 1: the preparation of yeast culture
1) seed liquor preparation: by candida utili (
candida utilis) CGMCC NO.7691 is seeded in liquid seed culture medium, obtain seed liquor, described liquid seed culture medium contains: molasses 10-20g/L, peptone 10-20g/L, sodium chloride 5-25g/L, pH 5.5-7.0, seed culture condition is: shaking table is cultivated, rotating speed 150-200r/min, temperature 28-32 DEG C, incubation time 18-24h;
2) liquid aerobic fermentation: seed liquor is seeded in the liquid fermentation medium of fermentation tank, amplify and cultivate, carry out aerobic fermentation, obtain liquid spawn solution, described liquid fermentation medium contains: molasses 40-80g/L, peptone 30-50g/L, sodium chloride 5-25 g/L, MgSO47H2O 1-5 g/L, pH 5.5-7.0, liquid aerobic fermentation condition is: inoculum concentration 10-20%, throughput 1-3L/min, speed of agitator 200-400r/min, fermentation tank pH automatic feedback regulating system is utilized to control zymotic fluid pH 5.5-7.0, remaining sugar concentration controls at 4-8wt%, fermentation temperature 25-35 DEG C, fermentation time 20-24h,
3) solid aerobic fermentation: by liquid spawn solution inoculum on sterilized solid medium, stone is in fermentation bed, carry out solid aerobic fermentation, described fermentation bed (existing apparatus) comprises the feeding device connected successively, installation for fermenting and blanking device, described installation for fermenting comprises 2-5 the chain-belt type fermentation interlayer be dislocatedly distributed from top to bottom, described solid medium contains: corn flour 45-65%, wheat bran 15-25%, dregs of beans 20-35%, solid aerobic fermentation condition: fermentation bed stone moisture 40-60%, inoculum concentration 10-20%, the fermentation temperature of each fermentation interlayer is 25-45 DEG C, throughput is 0.1-10 L/min, stirring is controlled at 3-6 time between yeast phase, aerobic fermentation time controling is at 24-36h,
4) solid anaerobic fermentation: control fermentation bed, close ventilation switch, carry out solid anaerobic fermentation, obtain solid fermentation product, solid anaerobic fermentation condition: the fermentation temperature of each fermentation interlayer is 25-45 DEG C, stuffiness, control stirring between yeast phase at 1-3 time, anaerobic fermentation time controling is at 12-18h;
5) solid fermentation product be dry, pulverize 40-60 order, obtain yeast culture.
Also brewer's yeast and rhodotorula can be adopted in this embodiment 1 to ferment.
Detect the yeast culture that embodiment 1 obtains, the leading indicator of detection has crude protein, coarse ash, crude fibre, yeast count,
β-glucan, mannosan, vomitoxin, AFB1.Detection method is: crude protein, detects by GB/T 6432-1994; Coarse ash, detects by GB/T6438-2007; Crude fibre, detects by GB/T 6434-2006; Yeast count, detects by GB/T13093-2006;
β-glucan, detects with Megazyme kit; Vomitoxin, GB/T 23503-2009 detects; AFB1, detects by GB 5009.24-2010.
Table 1 yeast culture active constituent content detects
| Project | Yikang is precious |
| Crude protein % | 18.50 |
| Coarse ash % | 3.07 |
| Crude fibre % | 2.8 |
| Yeast count | 62 ×10 8cfu/g |
| β-glucan % | 5.60 |
| Vomitoxin (ug/kg) | 153 |
| AFB1 (ug/kg) | 1.80 |
Embodiment 2: for preventing the preparation of the feed addictive of mastitis for milk cows and brucellosis
(1) raw material is taken by yeast culture 50%, cecropin 8%, the fruit of Chinese wolfberry 10%, date 10%, safflower 10%, the root of herbaceous peony 8% and montmorillonite 4%;
(2) the raw material mixing will taken, about being crushed to about 40-60 order, mixes thoroughly and obtains of the present invention for preventing the feed addictive of mastitis for milk cows and brucellosis.
Embodiment 3: for preventing the preparation of the feed addictive of mastitis for milk cows and brucellosis
(1) raw material is taken by yeast culture 60%, cecropin 6%, the fruit of Chinese wolfberry 6%, date 8%, safflower 6%, the root of herbaceous peony 10% and montmorillonite 4%;
(2) the raw material mixing will taken, about being crushed to about 40-60 order, mixes thoroughly and obtains of the present invention for preventing the feed addictive of mastitis for milk cows and brucellosis.
Embodiment 4: for preventing the preparation of the feed addictive of mastitis for milk cows and brucellosis
(1) raw material is taken by yeast culture 35%, cecropin 10%, the fruit of Chinese wolfberry 15%, date 15%, safflower 10%, the root of herbaceous peony 10% and montmorillonite 5%;
(2) the raw material mixing will taken, about being crushed to about 40-60 order, mixes thoroughly and obtains of the present invention for preventing the feed addictive of mastitis for milk cows and brucellosis.
Embodiment 5: for preventing the preparation of the feed addictive of mastitis for milk cows and brucellosis
(1) raw material is taken by yeast culture 55%, cecropin 1%, the fruit of Chinese wolfberry 12%, date 10%, safflower 8%, the root of herbaceous peony 10% and montmorillonite 4%;
(2) the raw material mixing will taken, about being crushed to about 40-60 order, mixes thoroughly and obtains of the present invention for preventing the feed addictive of mastitis for milk cows and brucellosis.
Embodiment 6: Animal experiment
In milk cattle cultivating base, Hangzhou, choose recessive mastitis and be diagnosed as the positive, the milk cow in lactation period of somatic number 500,000/mL 50, wherein said milk cow all shows as mammitis mild redness, in milk, cell body cell number is higher, and the output of milk is relatively low, and above-mentioned milk cow is divided into 2 groups at random, blank group 25, test group 25.Wherein organizing 1 is blank group, common, commercially available mixed feed of feeding, and test group adds the feed addictive (embodiment 2 obtains) described in 0.8% in the formula of the milk cow mixed fodder of routine.The basic components of above-mentioned each group of mixed feed is identical.Every day early, manually directly throw something and feed evening, each 50 grams/head, 60 days test periods, have studied the average milk production of duration of test, the parameter indexs such as Ruzhong somatic number, and the state of development of illness, concrete outcome sees the following form.
The result for the treatment of contrast of table 2 cow subclinical mastitis
| Group | Breast district | Cure newborn district | Cure rate/% |
| Test group | 42 | 28 | 66.67 |
| Blank group | 39 | 2 | 5.13 |
Find out (table 2) from result, experimental group 60 days recessive mastitis cure rates are 66.67%, and the blank group self-healing rate of not adding the feed addictive of this invention only has 5.13%.
The contrast of somatic number in table 3 raw milk
Test group treatment precursor number is at 50 ~ 2,000,000/mL, average body cell number reaches 1,100,000/mL, after treatment, somatic number drops to 5 ~ 500,000/mL, average body cell number 250,000/mL, average decline 850,000/mL, blank group treatment precursor number is at 56 ~ 2,100,000/mL, and average body cell number reaches 1,200,000/mL, body cell 60 ~ 2,900,000/mL after 60 days, average body cell number 1,800,000/mL.
The contrast of table 4 milk production of cow
Experimental Comparison finds, the test group output of milk is increased to 25.1kg from 24.0kg, adds 1.1kg, and the blank group output of milk is reduced to 23.7kg from 24.3kg, decreases 0.6kg.
Shown by above zooperal result, feed addictive of the present invention further develops to worsen for mastitis for milk cows initial stage illness has certain prevention effect for serious mammitis, namely can prevent further developing of mammitis; In addition, this feed addictive has certain healing effect for recessive mastitis, and can not affect the quality of dairy products and the output of cow's milk.
Equally, test as identical in embodiment 6 is carried out to the feed addictive that embodiment 3-5 obtains, finally also can reach test effect as similar in embodiment 6, its 60 days recessive mastitis cure rates all can reach more than 66%, and can not affect the quality of dairy products and the output of cow's milk.
Embodiment 7: Animal experiment
In milk cattle cultivating base, Hangzhou, choose 150 cow heads, utilize kit to detect serum, wherein 136 is that brucellosis is positive, 49 feminine genders, to feed common, commercially available mixed feed, and add the feed addictive (embodiment 3 obtains) described in 0.8%, every day early, evening manually directly throws something and feeds, each 100 grams/head, 60 days test periods, have studied the state of development of duration of test milk cow brucellosis illness, concrete outcome sees the following form.
Table 5 adds the impact of additive on milk cow brucellosis in dairy cow diet
It is bad that milk cow trouble B.abortus shows as nutrition intake after being ill, and nutrient absorption is bad, and major embodiment is propagating system and disease of digestive system, occurs weak sick matter, milk cow skeletonize, and output is low, and mortality is high.Cryptorrhea causes nutrient consumption to increase simultaneously, and metabolic disorder, shows as feed conversion rate low, cultivation inefficiency.These cattle farm part milk cow chronic wasting disease cows occurred frequently are fed this invention feed addictive, detection is fed front and back milk cow chronic wasting disease incidence, in table, data show, the milk cow chronic wasting disease positive rate adding this invention feed addictive is reduced to 57.30% from 73.51%, have dropped 16.21%.
Equally, with 5 feed addictives obtained, test as identical in embodiment 7 is carried out to embodiment 2,4, finally also can reach test effect as similar in embodiment 7, have obvious curative effect to milk cow chronic wasting disease.
Raw material of the present invention all commercially, can be only specific embodiments of the invention disclosed in above, but the present invention is not limited thereto, the technical staff in any field can think change, all should drop within protection scope of the present invention.
Claims (6)
1., for preventing a feed addictive for mastitis for milk cows and brucellosis, it is characterized in that being made up of the raw material of following percentage by weight:
Yeast culture 35-60%, cecropin 1-10%, fruit of Chinese wolfberry 8-15%, date 8-15%, safflower 5-10%, root of herbaceous peony 5-10% and montmorillonite 4-8%.
2. a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis as claimed in claim 1, it is characterized in that being made up of the raw material of following percentage by weight:
Yeast culture 45-55%, cecropin 2-8%, fruit of Chinese wolfberry 10-13%, date 10-13%, safflower 6-8%, root of herbaceous peony 6-8% and montmorillonite 5-7%.
3. a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis as claimed in claim 1, it is characterized in that being made up of the raw material of following percentage by weight:
Yeast culture 48-52%, cecropin 4-6%, fruit of Chinese wolfberry 10-12%, date 10-12%, safflower 7-8%, root of herbaceous peony 7-8% and montmorillonite 6-7%.
4. a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis as described in claim 1,2 or 3, is characterized in that described yeast culture is candida utili culture, brewer's yeast culture or rhodotorula culture.
5. a kind of for preventing the feed addictive of mastitis for milk cows and brucellosis as claimed in claim 4, it is characterized in that described yeast culture is obtained by following steps:
1) seed liquor preparation: by candida utili (
candida utilis) CGMCC NO.7691, brewer's yeast or rhodotorula be seeded in liquid seed culture medium, obtain seed liquor, described liquid seed culture medium contains: molasses 10-20g/L, peptone 10-20g/L, sodium chloride 5-25g/L, pH 5.5-7.0, seed culture condition is: shaking table is cultivated, rotating speed 150-200r/min, temperature 28-32 DEG C, incubation time 18-24h;
2) liquid aerobic fermentation: seed liquor is seeded in the liquid fermentation medium of fermentation tank, amplify and cultivate, carry out aerobic fermentation, obtain liquid spawn solution, described liquid fermentation medium contains: molasses 40-80g/L, peptone 30-50g/L, sodium chloride 5-25 g/L, MgSO47H2O 1-5 g/L, pH 5.5-7.0, liquid aerobic fermentation condition is: inoculum concentration 10-20%, throughput 1-3L/min, speed of agitator 200-400r/min, fermentation tank pH automatic feedback regulating system is utilized to control zymotic fluid pH 5.5-7.0, remaining sugar concentration controls at 4-8wt%, fermentation temperature 25-35 DEG C, fermentation time 20-24h,
3) solid aerobic fermentation: by liquid spawn solution inoculum on sterilized solid medium, stone is in fermentation bed, carry out solid aerobic fermentation, described fermentation bed comprises the feeding device connected successively, installation for fermenting and blanking device, described installation for fermenting comprises 2-5 the chain-belt type fermentation interlayer be dislocatedly distributed from top to bottom, described solid medium contains: corn flour 45-65%, wheat bran 15-25%, dregs of beans 20-35%, solid aerobic fermentation condition: fermentation bed stone moisture 40-60%, inoculum concentration 10-20%, the fermentation temperature of each fermentation interlayer is 25-45 DEG C, throughput is 0.1-10 L/min, stirring is controlled at 3-6 time between yeast phase, aerobic fermentation time controling is at 24-36h,
4) solid anaerobic fermentation: control fermentation bed, close ventilation switch, carry out solid anaerobic fermentation, obtain solid fermentation product, solid anaerobic fermentation condition: the fermentation temperature of each fermentation interlayer is 25-45 DEG C, stuffiness, control stirring between yeast phase at 1-3 time, anaerobic fermentation time controling is at 12-18h;
5) solid fermentation product be dry, pulverize 40-60 order, obtain yeast culture.
6. a kind of for preventing the preparation method of the feed addictive of mastitis for milk cows and brucellosis as described in claim 1,2 or 3, is characterized in that comprising following processing step:
(1) raw material is taken in proportion;
(2) the raw material mixing will taken, about being crushed to about 40-60 order, mixes thoroughly and obtains of the present invention for preventing the feed addictive of mastitis for milk cows and brucellosis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410227089.5A CN104206789B (en) | 2014-05-27 | 2014-05-27 | It is a kind of to be used to prevent mastitis for milk cows and the feed addictive of brucellosis and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410227089.5A CN104206789B (en) | 2014-05-27 | 2014-05-27 | It is a kind of to be used to prevent mastitis for milk cows and the feed addictive of brucellosis and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104206789A true CN104206789A (en) | 2014-12-17 |
| CN104206789B CN104206789B (en) | 2017-09-12 |
Family
ID=52089003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410227089.5A Active CN104206789B (en) | 2014-05-27 | 2014-05-27 | It is a kind of to be used to prevent mastitis for milk cows and the feed addictive of brucellosis and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104206789B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104824374A (en) * | 2015-04-30 | 2015-08-12 | 新昌县大成生物科技有限公司 | Feed additive for treating dairy cow brucellosis |
| CN105410368A (en) * | 2015-11-22 | 2016-03-23 | 衡阳市富矿饲料添加剂有限公司 | Feed additive for preventing dairy cattle subclinical mastitis and preparation method thereof |
| CN105494969A (en) * | 2015-12-30 | 2016-04-20 | 内蒙古自治区农牧业科学院 | Cow fodder and feeding method capable of reducing somatic number of milk |
| CN106819435A (en) * | 2017-01-22 | 2017-06-13 | 长治市雄丰清真养殖屠宰有限公司 | A kind of feed of prevention cattle and sheep brucellosis |
| CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
| CN108813172A (en) * | 2018-06-20 | 2018-11-16 | 濮阳市天然林牧业科技开发有限公司 | A kind of cow feed additive |
| CN108887486A (en) * | 2018-06-20 | 2018-11-27 | 濮阳市天然林牧业科技开发有限公司 | A kind of dairy cow nutrition supplement feed |
| CN113475461A (en) * | 2021-08-11 | 2021-10-08 | 云南农业大学 | Comprehensive prevention and treatment method for breeding diseases of cattle and sheep |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59179036A (en) * | 1983-03-29 | 1984-10-11 | Nippon Sharyo Seizo Kaisha Ltd | Preparation of protein supplement feed from green grass |
| CN101914460A (en) * | 2010-07-13 | 2010-12-15 | 广州市博善生物饲料有限公司 | Method for fermenting phodotorula benthica culture |
| KR101130911B1 (en) * | 2010-05-27 | 2012-03-28 | 김광부 | The manufacturing method of the feedstuff to contain a kudzu |
| CN102643864A (en) * | 2012-04-27 | 2012-08-22 | 广州市富泉生物科技有限公司 | Process for preparing yeast cultures |
| CN103340312A (en) * | 2013-07-11 | 2013-10-09 | 曾勇 | Feed additive for preventing dairy cow mastitis |
| CN103431198A (en) * | 2013-09-06 | 2013-12-11 | 山东凤祥股份有限公司 | Poultry feed additive and poultry feed |
| CN103468594A (en) * | 2013-07-31 | 2013-12-25 | 浙江科峰生物技术有限公司 | Candidautilis strain and application thereof |
| CN103609864A (en) * | 2013-11-29 | 2014-03-05 | 浙江科峰生物技术有限公司 | Method for producing bean pulp feed additive |
| CN103768035A (en) * | 2014-01-16 | 2014-05-07 | 中国农业科学院兰州畜牧与兽药研究所 | Antimicrobial peptide effervescent tablets and preparation method and application thereof |
-
2014
- 2014-05-27 CN CN201410227089.5A patent/CN104206789B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59179036A (en) * | 1983-03-29 | 1984-10-11 | Nippon Sharyo Seizo Kaisha Ltd | Preparation of protein supplement feed from green grass |
| KR101130911B1 (en) * | 2010-05-27 | 2012-03-28 | 김광부 | The manufacturing method of the feedstuff to contain a kudzu |
| CN101914460A (en) * | 2010-07-13 | 2010-12-15 | 广州市博善生物饲料有限公司 | Method for fermenting phodotorula benthica culture |
| CN102643864A (en) * | 2012-04-27 | 2012-08-22 | 广州市富泉生物科技有限公司 | Process for preparing yeast cultures |
| CN103340312A (en) * | 2013-07-11 | 2013-10-09 | 曾勇 | Feed additive for preventing dairy cow mastitis |
| CN103468594A (en) * | 2013-07-31 | 2013-12-25 | 浙江科峰生物技术有限公司 | Candidautilis strain and application thereof |
| CN103431198A (en) * | 2013-09-06 | 2013-12-11 | 山东凤祥股份有限公司 | Poultry feed additive and poultry feed |
| CN103609864A (en) * | 2013-11-29 | 2014-03-05 | 浙江科峰生物技术有限公司 | Method for producing bean pulp feed additive |
| CN103768035A (en) * | 2014-01-16 | 2014-05-07 | 中国农业科学院兰州畜牧与兽药研究所 | Antimicrobial peptide effervescent tablets and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 朱振鹏等: "天蚕素抗菌肽在动物生产上的研究进展", 《广东饲料》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104824374A (en) * | 2015-04-30 | 2015-08-12 | 新昌县大成生物科技有限公司 | Feed additive for treating dairy cow brucellosis |
| CN105410368A (en) * | 2015-11-22 | 2016-03-23 | 衡阳市富矿饲料添加剂有限公司 | Feed additive for preventing dairy cattle subclinical mastitis and preparation method thereof |
| CN105494969A (en) * | 2015-12-30 | 2016-04-20 | 内蒙古自治区农牧业科学院 | Cow fodder and feeding method capable of reducing somatic number of milk |
| CN106819435A (en) * | 2017-01-22 | 2017-06-13 | 长治市雄丰清真养殖屠宰有限公司 | A kind of feed of prevention cattle and sheep brucellosis |
| CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
| CN108813172A (en) * | 2018-06-20 | 2018-11-16 | 濮阳市天然林牧业科技开发有限公司 | A kind of cow feed additive |
| CN108887486A (en) * | 2018-06-20 | 2018-11-27 | 濮阳市天然林牧业科技开发有限公司 | A kind of dairy cow nutrition supplement feed |
| CN113475461A (en) * | 2021-08-11 | 2021-10-08 | 云南农业大学 | Comprehensive prevention and treatment method for breeding diseases of cattle and sheep |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104206789B (en) | 2017-09-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104206789B (en) | It is a kind of to be used to prevent mastitis for milk cows and the feed addictive of brucellosis and preparation method thereof | |
| CN102488117B (en) | Combination used for preventing and treating newborn pig diarrhea and preparation method and application thereof | |
| CN103798553B (en) | Environment-friendly pig feed additive and preparation method thereof | |
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| CN103005159A (en) | Preparation method of ginkgo leaf biological feed additive | |
| CN104293697A (en) | Chicken feed probiotic agent containing enterococcus faecalis and preparation method of chicken feed probiotic agent | |
| CN102885202A (en) | Novel poultry micro-ecological preparation and preparation method thereof | |
| CN105076862A (en) | Anti-stress premix capable of promoting growth of piglets and preparation method of anti-stress premix | |
| CN108402320A (en) | A kind of composite probiotics preparations and its application in pig starter feed | |
| CN105994941A (en) | Non-antibiotic feed prepared through microbial fermentation | |
| CN112136965B (en) | Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof | |
| CN106922997A (en) | A kind of pig feed additive of efficient gastrointestinal mucosa repair function | |
| CN109770085A (en) | A kind of biological feed additive and preparation method thereof prevented or treat diarrhea of pigs | |
| CN107821789A (en) | A kind of biologic ferment for improving fishes and shrimps intestinal health degree and its preparation method and application | |
| CN104543410B (en) | Disease-resistant pig feed additive of a kind of health care and preparation method thereof | |
| CN110892944A (en) | Weaned pig feed additive capable of replacing antibiotics and application thereof | |
| CN102907585A (en) | Probiotics and vitamin premix and mixture for middle pig | |
| CN110292114A (en) | A kind of fermented feed that Yak Growth performance can be improved | |
| CN101874812B (en) | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea | |
| CN101999554A (en) | Special animal nutrient for milk cows and preparation method thereof | |
| CN103315191B (en) | Swine immunopotentiator and its preparation method | |
| CN107961260A (en) | Treatment, the medicament for preventing birds coliform diarrhea disease and preparation method thereof | |
| CN102754733A (en) | Novel micro-ecologic preparation for pig and preparation method thereof | |
| CN102106487A (en) | Biological fermentation feed for laying chickens and production method | |
| RU2468594C1 (en) | Method for improvement of livability and performance of piglets after weaning |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |