CN102488117B - Combination used for preventing and treating newborn pig diarrhea and preparation method and application thereof - Google Patents
Combination used for preventing and treating newborn pig diarrhea and preparation method and application thereof Download PDFInfo
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- CN102488117B CN102488117B CN 201110436779 CN201110436779A CN102488117B CN 102488117 B CN102488117 B CN 102488117B CN 201110436779 CN201110436779 CN 201110436779 CN 201110436779 A CN201110436779 A CN 201110436779A CN 102488117 B CN102488117 B CN 102488117B
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a combination used for preventing and treating newborn piglet diarrhea and a preparation method and application thereof. In percent by weight, the product comprises 88-98% of attapulgite ultra-fine powder, 0.1-3% of organic acid, 0.1-4% of probiotic, 0.05-2% of oligosaccharide, 0.05-1.5% of vitamin C and 0.15-3% of crane sugar. The combination disclosed by the invention is diluted into solution with the mass fraction of 0.05-3% with drinking water, the solution is used for feeding a piglet in an oral or drinking water manner, and the newborn piglet diarrhea caused by multiple factors can be effectively prevented and treated, thus the combination has the effects of improving immunocompetence of the piglet, improving antioxidant function, regulating intestinal microecology and protecting intestinal shape and can improve survival rate of the piglet, improve production performance of the piglet and relieve weaned stress on the piglet. The combination disclosed by the invention also has the characteristics of simple and convenient application mode, high efficiency, available raw materials, simple preparation and the like.
Description
Technical field
The invention belongs to the animal health field, be specifically related to a kind of compound formulation of preventing and treating grice diarrhoea and its preparation method and application.
Background technology
China is the big country of raising pigs, and the pig-breeding amount ranks first in the world.State Statistics Bureau's publish data shows, 2009-2010, and national live pig is delivered respectively 64,570 ten thousand and 66,700 ten thousand for sale, and still, China's Swine Production level is lower, exists the problems such as the piglet death rate is high, the unit sow yields poorly.According to statistics, the nest that China raises pigs is all lived young several 9～9.5, and every sow year is produced 16～17 of big porkers; And the nest that cultivates developed country is all lived young several 10～12, and every sow year is produced 20～22 of big porkers; It is 4,729 ten thousand that China sow in 2010 is raised a number, if by dead 3 calculating in every sow litter, the annual dead piglet of China is about 14,200 ten thousand.Compare with developed country cultivation level, the Main Gaps of China's pig industry is that the piglet death rate is high, and wherein diarrhoea is to cause main causes of death.Grice diarrhoea is a kind of typical multifactor property disease under the intensive pig production working condition, can cause that piglet is dead, piglet survival ratio reduces, the price of deed descends, late growing stage slowly or stagnations of growing, become one of key factor that the restriction pig industry develops.It is reported, the piglet death rate of China is generally 11%～25%, the death that the piglet that dies of illness causes for the diarrhoea relevant disease more than 80%.The grice diarrhoea priming factors mainly comprises infectiousness cause of disease and the non-infective disease cause of disease two aspects: in causing the infectiousness cause of disease of grice diarrhoea, virus mainly contains Porcine epidemic diarrhea virus, transmissible gastro-enteritis virus and rotavirus, and bacterium mainly contains shigella dysenteriae, comma bacillus, Escherichia coli, salmonella and clostridieum welchii etc.; Non-infectious factor comprises mainly that stress factor, the piglet digestive function such as wean are complete, daily ration antigen is irritated, protein Excessive Intake and some trophic factors shortage etc.
At present, the control grice diarrhoea generally adopt antibiotic or other medicines carry out oral, drinking-water or the injection, or add in feed that medicine prevents, treatment and alleviating diarrhoea, but the long-term use of antibiotic etc can make pathogenic microorganism produce drug resistance, drug resistant gene can transmit or shift mutually between the bacterium of the mankind, animal and the ecosystem, causes animal and human's class rear treatment difficulty that catches even failed; In addition, the too much grievous injury piglet of drug use allelotaxis causes immunosupress, thereby affects piglet late growing stage and immunologic function; Moreover a large amount of uses of medicine will cause livestock products medicament residue problem, have a strong impact on food security, threaten human health.Therefore, in feed, the forbidding antibiotic has become certainty, in the urgent need to developing Substitutes For Antibiotic or other technical measures to prevent and treat Animal diseases.Recent years, the research of relevant Substitutes For Antibiotic in preventing and treating Animal diseases is a lot, mainly contain Chinese herbal medicine or its extract, organic acid, oligosaccharides, probio and other organic-inorganic antiseptic etc., these additives all have certain antibiotic or antivirus action, have to a certain extent the effect of control grice diarrhoea, have higher security.But when being applied to prevent and treat grice diarrhoea, multiple feed addictive all has some problems: due to the physilogical characteristics of early weaning piglet and the Chinese herbal medicine drug effect is slow and dosage is bigger than normal, palatability is poor, the aspects such as Chinese herbal medicine effective ingredients, active ingredient interphase interaction, drug effect, quality control and mechanism of action also lack quantizating index and the further investigation of modern science, have limited the Chinese herbal medicine application in weanling pig in early days; Organic acid makes an addition to can suppress the pathogenic microorganism growth in piglet diet, but its action effect is subjected to the impact of daily ration component, piglet age in days, kinds of organic acids and the many factors such as addition, feeding and management, make the effect of organic acid in the control grice diarrhoea unstable, in use existence release is too fast, addition large and the high in cost of production problem, simultaneously, make an addition to problems such as also having part nutrient stability in the destruction feed, easy moisture absorption caking, etching mechanism in feed; Use the probioticses such as oligosaccharides and probio can regulate the alimentary canal microbial balance, but the impact on pathogenic entero becteria, normal flora and animal body immunologic function is still waiting further research, simultaneously, such feed addictive only has certain preventive and therapeutic effect to bacillary and irritability diarrhoea, to virus diarrhea without direct effect; The inorganic antiseptic high doses such as copper, zinc or arsenic preparation pollute the environment when using, and also have residue problem, only have bacteriostasis simultaneously, to the direct prevention effect of virus nothing.When these novel fodder additives add in feed feeding animals to, due to characteristics such as feed digestion time in animal are long, influence factor is more, make it to have utilization ratio lower, take effect comparatively slowly, the problem such as action function is single and action effect is unstable.
Summary of the invention
For control grice diarrhoea the deficiencies in the prior art and the blank that makes up the market Related product, the object of the present invention is to provide a kind of compound formulation of preventing and treating grice diarrhoea.This compound formulation can be prevented and treated grice diarrhoea effectively by oral or the water way piglet of feeding, and has the weaned piglets of improvement, alleviates the action functions such as ablactation stress.
Another object of the present invention is to provide a kind of preparation method who prevents and treats the compound formulation of grice diarrhoea.
Another purpose of the present invention is to provide the application of above-mentioned compound formulation in the control grice diarrhoea.
Technical scheme of the present invention can be achieved through the following technical solutions:
A kind of compound formulation of preventing and treating grice diarrhoea, by the quality percentage composition, comprise attapulgite clay micropowders 88%～98%, organic acid 0.1%～3%, probio 0.1%～4%, oligosaccharides 0.05%～2%, vitamin C 0.05%～1.5% and sucrose 0.15%～3%.
The compound formulation of above-mentioned control grice diarrhoea, it is that described attapulgite clay micropowders is more than attapulgite clay ultramicro grinding to 600 order, described attapulgite clay is the original soil that directly selecting and purchasing gets from natural crystal, can be also the modified product of processing through Overheating Treatment, acid treatment, sodium salt processing, shitosan processing or magnetic.
The compound formulation of above-mentioned control grice diarrhoea, its quality percentage composition that is attapulgite in described attapulgite clay is not less than 60%; The quality percentage composition that is preferably attapulgite in described attapulgite clay is not less than 85%.
The compound formulation of above-mentioned control grice diarrhoea, it is that described organic acid is a kind of or any combination in citric acid, fumaric acid and lactic acid.Described oligosaccharides is a kind of or any combination in xylo-oligosaccharide and chitosan oligomer.
The compound formulation of above-mentioned control grice diarrhoea, it is by the quality percentage composition, described probio comprises bacillus 50%～80% and lactic acid bacteria 20%～50%.
The compound formulation of above-mentioned control grice diarrhoea, it is described bacillus living content〉1 * 10
10CFU/g, described viable lactic acid bacteria content〉3 * 10
10CFU/g.
The compound formulation of above-mentioned control grice diarrhoea, it is that described bacillus is a kind of or any combination in bacillus licheniformis, bafillus natto, bacillus subtilis.
The compound formulation of above-mentioned control grice diarrhoea, its preparation method fully mixes organic acid, probio, oligosaccharides, vitamin C, sucrose and attapulgite clay micropowders for by described content proportioning.
The preparation method of the compound formulation of above-mentioned control grice diarrhoea, by described content proportioning, organic acid, probio, oligosaccharides, vitamin C, sucrose and attapulgite clay micropowders are fully mixed, namely get the compound formulation product of control grice diarrhoea.
Above-mentioned compound formulation is used in the control grice diarrhoea.During use, with clean drinking water, the compound formulation product being diluted to mass fraction is 0.05%～3% solution, after stirring by oral or the water way piglet of feeding; Also compound formulation can be mixed the piglet of feeding in feed.
Enteron aisle is the organ of digesting and assimilating of animal maximum, also immunity and endocrine organ maximum in body, as contact external microbe and the maximum position of other harmful substance, enteron aisle is the main door of pathogen invasion and nuisance invasion and attack, is again the first barrier that stops cause pathogeny imcrobe infection and nuisance to pollute.The bacterium, virus and secreted toxin, ammonia or the harmful substances such as organic amino compounds, heavy metal that enter animal alimentary canal will be damaged intestinal mucosa and the epithelial cell of animal, affect the resistance against diseases of animal, cause the various diseases that comprises diarrhoea.In addition; animal is in wean, turning the processes such as group, high temperature and inoculation; to produce various stress; weanling pig particularly; normal easily diarrhea, this is mainly that the enteron aisle that stress cause is impaired and cause due to pathogenic infection, therefore; take effective measures the protection enteron aisle, can effectively prevent grice diarrhoea.
In the present invention, described attapulgite clay micropowders is more than attapulgite clay ultramicro grinding to 600 order, the fine particle of formation take less than the particle of 20 μ m particle diameters as main.The essential mineral composition of attapulgite clay is attapulgite, be a kind of have a layer chain crystal structure contain Shuifu County's alumina magnesia silicate, be under the jurisdiction of sepiolite group on mineralogy.The structure of attapulgite can be divided into 3 layers, and the up and down is two-layer is the Si-O tetrahedral structure, and the centre is one deck Mg-O octahedral structure, and these construction units are staggered by the grid form, form bar-shaped, filamentary structure.Based on its unique morphosis, larger specific area, special ionic forms, make attapulgite clay have the characteristics such as good selection absorption, cation exchange, load, suspension, rheological characteristic, heat endurance, all be widely used in each fields such as environmental protection, medicine, agricultural and chemical industry.The ground such as China Jiangsu, Gansu, Anhui abound with attapulgite clay, are the whole world the abundantest countries of attapulgite clay resource.Attapulgite clay has very strong covering power to gastrointestinal mucosal after entering animal intestines and stomach; has powerful adsorptivity; adsorbable various viruses, the bacterium interior with suppressing animal gastrointestinal tract and the toxin that produces thereof; and adsorbable ammonia or the harmful substances such as organic amino compounds, heavy metal ion; guarantee to have good physiological environment in alimentary canal; promote the reparation of barrier of gastric mucosa, regulating intestinal canal colony balance, thereby protection enteron aisle and mucous membrane function.It is reported, attapulgite clay or its partially modified product can adsorb the microorganisms such as Escherichia coli, salmonella, can effectively adsorb the toxin in enteron aisle, as cholera toxin and ECT.In addition, attapulgite clay has very strong water imbibition, and its contained exchangeable cations also can be animal the several kinds of mineral elements such as magnesium, iron, zinc are provided, and has the effect that promotes metabolism, strengthens health.Therefore, the attapulgite clay product has good enteron aisle and mucosa protection, and the diarrhoea that the Different types of etiopathogenises that animal is occured causes all has regulating and controlling effect preferably.In American-European countries, present existing multiple pharmaceutical preparation take attapulgite or its like product montmorillonite as main component, be mainly used in treatment diarrhoea and gastrointestinal disease etc., as the good Smecta(dioctahedral smectites of disease effects such as control diarrhoea in China's fast sale) main component of product is montmorillonite, but its price is more expensive and need import.Hospital of China PLA develops the Novel alimentary canal mucosal protective agent take the abundant attapulgite clay of Domestic Resources as primary raw material; the clinical trial result shows; it can be used as one of active drug for the treatment of diarrhoea; gastrointestinal mucosal had significant protective effect; also meet six standards of anti-diarrheal that WHO formulates, namely efficient, can be oral, can with the ORT compatibility with, not by intestinal absorption, do not affect intestinal absorption ability and can resist a series of enteron aisle cause of diseases.Show according to the inventor's repeatedly animal experiment, attapulgite clay has certain protective effect to animal intestinal form and health.In addition, attapulgite clay has good suspension, can form the suspension of good stability in the aqueous solution, can be made into oral or the drinking-water product; Attapulgite clay has load-carrying properties and powerful bearing capacity preferably, and has good rheological characteristic, can be used as the carrier of multiple feed addictive; After adopting superfine communication technique that attapulgite clay is made micropowders, can improve its area coverage, absorption property and suspendability.Therefore, in the present invention with the superfine powder of attapulgite clay original soil or its modified product as main component, be mixed with piglet patent intestinal protection, by oral or the water way piglet of feeding, with the control grice diarrhoea.Attapulgite clay described in the present invention is the high-quality original soil that directly selecting and purchasing gets from natural crystal, can be also the modified product of processing through Overheating Treatment, acid treatment, sodium salt processing, shitosan processing, magnetic; Wherein in attapulgite clay, the content of attapulgite is not less than 60%, is preferably by purification processes to make the content of attapulgite be not less than 85%.
In the present invention, described organic acid is a kind of or any combination in citric acid, fumaric acid and lactic acid.Organic acid is mainly used in young animal, its main purpose is for the replenishing gastric acid deficiency, reduce enteron aisle pH value, to the secretion that suppresses pathogenic microorganism growth, the enzyme that stimulates digestion, promote digestion and improve the aspect such as intestinal environment all have positive meaning, can prevent the generation of diseases such as suffering from diarrhoea, simultaneously, also have absorption and the effects such as utilization that improve palatability, ptyalize, promote trace element.Organic acid commonly used has citric acid, fumaric acid, lactic acid, malic acid and fumaric acid etc. now.Organic acid not only can be applicable to studies show that in feed, adds organic acid and can kill bacterium in drinking-water system and alimentary canal in drinking-water, improves the digestibility of protein, and the microbiota in regulating intestinal canal.Citric acid is a kind of organic acid that has than the strong acid flavor, is the synergist of antioxidant; Fumaric acid is a kind of sour unsaturated dicarboxylic acid, has dissolubility preferably in water, except possessing the organic acid functional characteristic, also has the effect of non-oxidizability and reinforcement vitamin stability; Lactic acid is a kind of organic acid with particular tastes, can be used as anticorrisive agent and uses, it is reported, lactic acid can suppress the harmful bacteria such as Escherichia coli and salmonella in intestine of young pigs growth, improve weaned piglets.In the present invention by organic acid self function and with other composition between synergy, can prevent and reduce grice diarrhoea, improve digestion and absorption function, alleviate stress, thereby the raising weaned piglets.The present invention uses organic acid to make oral or the drinking-water product, overcome organic acid and added the shortcomings such as affecting of producing the part nutrient is stable, easy moisture absorption caking, etching mechanism in feed, simultaneously, absorption and bearing function by means of attapulgite clay, organic acid is slowly discharged in alimentary canal, make its effect be continued until the enteron aisle back segment, play good acidifying and bacteriostasis effect.
Studies show that, probio is introduced alimentary canal as useful inoculating microbe, replenishes the normal microorganism that reduces or lack in enteron aisle on value volume and range of product, with harmful bacteria competition ecological niche, and can produce antibacterial material, suppress the growth and breeding of pathogenic bacteria, the colony balance of adjustment and recovery enteron aisle; Add a certain amount of probio in feed or drinking-water, all have certain effect improving immunity, control grice diarrhoea, alleviate ablactation stress and improve the aspects such as piglet survival ratio.Use the compound probiotic that comprises bacillus and lactic acid bacteria in the present invention, can bring into play the synergy of many bacterial classifications.The characteristics such as that functional oligosaccharide has is stable, safety non-toxic, it can not be digested by the digestive ferment of animal itself, can be combined with pathogen, the field planting in animal body of blocking-up pathogenic microorganism, can be in enteron aisle as the substrate of the beneficial bacteriums such as Bifidobacterium, the propagation of promotion beneficial bacterium suppresses the harmful bacteria growth simultaneously, uses oligosaccharides can improve immune performance in animal produces, reduces the effects such as the death rate and promotion growth performance.The present invention unites use probio and oligosaccharides, be intended to bring into play simultaneously both double actions, by reducing the field planting of pathogenic microorganism, promote the field planting in animal intestinal of exogenous viable bacteria, optionally stimulate the Growth and reproduction of one or more beneficial bacteriums, improve the formation of microbial population of animal intestinal tract, regulate the little ecology of alimentary canal, improve immunocompetence and guarantee host health, thereby effectively preventing grice diarrhoea.Probio described in the present invention by the quality percentage composition, comprises bacillus 50%～80% and lactic acid bacteria 20%～50%, and wherein bacillus is a kind of or any combination in bacillus licheniformis, bafillus natto, bacillus subtilis.Oligosaccharides described in the present invention is a kind of or any combination in xylo-oligosaccharide and chitosan oligomer.
Vitamin C has another name called ascorbic acid, is a kind of antioxidant, have very strong anti-radical action, have simultaneously anti-stress ability preferably, can react the concentration that axle is regulated the stress hormone cortisol by hypothalamic-pituitary-adrenal, improve human body immune function, reach anti-stress purpose.It is reported, after wean, piglet blood plasma level of vitamin C sharply descends, and namely ablactation stress makes piglet increase ascorbic requirement, and this moment, the appropriate vitamin C that replenishes had obvious anti-stress effect.Vitamin C is water soluble vitamin, and compound formulation product of the present invention is fed to piglet by oral or drinking-water, can improve ascorbic functioning efficiency.
The sense of taste of pig is more responsive, especially sweet taste is liked most in sour, sweet, bitter, peppery, salty 5 kinds of sense of taste.Sweetener has the modification masking ability to peculiar smell, can improve the animal sense of taste, promotes digesting and assimilating and utilizing of feed, improves feed intake and amount of drinking water, especially young animal and shows more obviously with the animal that is in stress situation.The research discovery, pig has strong preference to the sucrose glassware for drinking water; More near the sucrose flavor, quality is better when sweet taste, and pig is just more liked; The cane-sugar taste of molasses type is the favorite tastes of animal such as sucking pig, piglet and calf.It is sweetener that the present invention selects sucrose, improves piglet to the palatability of product.
Except specified otherwise, the raw material that the present invention relates to and reagent is commercially available getting all.
In the present invention, the preparation method of the compound formulation of described control grice diarrhoea, be by aforesaid addition or content proportioning, organic acid, probio, oligosaccharides, vitamin, sucrose and attapulgite clay micropowders are fully mixed, namely get the compound formulation product of control grice diarrhoea.
It is 0.05%～3% solution that the compound formulation of control grice diarrhoea of the present invention is diluted to mass fraction with clean drinking water with it, after stirring by oral or the water way piglet of feeding, perhaps compound formulation is mixed the piglet of feeding in feed, can effectively prevent and treat the grice diarrhoea that many factors causes, improve the piglet survival rate.
Beneficial effect of the present invention is:
1, according to the piglet physilogical characteristics and cause the reason of diarrhoea; based on characteristics such as the enteron aisle defencive function of attapulgite clay and bearing capacitys; adopt multi-functional, multi-level technical measures; the inventor's technological innovation (attapulgite clay superfine communication technique) is carried out integrated assembling in conjunction with multiple single feed additive; a kind of compound formulation of preventing and treating grice diarrhoea is provided; can by oral or the water way piglet of feeding, fill up the blank of market similar action product.
2, the compound formulation of the control grice diarrhoea take the attapulgite clay micropowders as main component, after entering the piglet stomach and intestine by oral or water way, gastrointestinal mucosal had very strong covering power, can improve the defense function of Mucosa Barrier, then keep gastral normal physiological function, simultaneously can effectively adsorb and remove the bacterium of causing a disease, virus and other harmful substance, and has a multiple bioactive functions of various new feed addictive, not only effective to bacterial diarrhea, the diarrhoea that the stress factor such as viral and wean are caused also has obvious prevention effect.
3, this compound formulation product have improve the piglet immunological ability, improve anti-oxidation function, the effect of the little ecology of regulating intestinal canal and protection Intestinal Morphology, can improve the piglet survival rate, improve weaned piglets, improve piglet health, alleviate stress of baby pigs caused ablaction etc.
4, add to like product and be applied in feed compare in piglet, this compound formulation product can be by the oral or piglet of feeding that drinks water, application mode is easy, has more direct, efficient effect, due to carrying, absorption and the slow releasing function of attapulgite clay, can make organic acid isoreactivity material play more efficient and permanent effect simultaneously.
5, as the attapulgite clay aboundresources of product main component, product configuration is easy to implement the method, and is easy and simple to handle, applicable to large-scale production and application.
The specific embodiment
The invention will be further described below in conjunction with embodiment, but the present invention is not limited.
Embodiment 1: the preparation of the compound formulation of control grice diarrhoea (drinking-water)
With the compound formulation quality of control grice diarrhoea of the present invention by 100 parts, ratio of quality and the number of copies as described below carries out proportioning and fully mixes, and namely obtains the compound formulation of control grice diarrhoea (drinking-water): attapulgite clay micropowders 95%, organic acid 1%, probio 1.2%, oligosaccharides 0.5%, vitamin C 0.8% and sucrose 1.5%.Wherein the attapulgite clay micropowders is attapulgite clay ultramicro grinding to 600 order, and attapulgite clay used is the high-quality original soil that directly selecting and purchasing gets from natural crystal, and the content of contained attapulgite is not less than 65%; Organic acid used comprises 70% citric acid and 30% fumaric acid by the quality percentage composition; Probio used is by the quality percentage composition, comprise bacillus 70% and lactic acid bacteria 30%, wherein bacillus is comprised of 50% bafillus natto, 40% bacillus subtilis and 10% bacillus licheniformis, bafillus natto by Yixing City section support one's family thing Co., Ltd provide (viable bacteria content〉1.6 * 10
10CFU/g), bacillus subtilis by nine divisions of China in remote antiquity, Nanjing interconnected agriculture and animal husbandry Science and Technology Ltd. provide (viable bacteria content〉1 * 10
10CFU/g), bacillus licheniformis by the U.S. refined come large pharmaceutical factory provide (commodity benefit poultry by name is precious, viable bacteria content〉2 * 10
10CFU/g), lactic acid bacteria (viable bacteria content〉3 * 10
10CFU/g) be that 10 times of the lactic acid bacteria product of inferior core (Nanjing) bio tech ltd dilutions are got; Oligosaccharides used is xylo-oligosaccharide.
Embodiment 2: the preparation of the compound formulation of control grice diarrhoea (oral)
With the compound formulation quality of control grice diarrhoea of the present invention by 100 parts, ratio of quality and the number of copies as described below carries out proportioning and fully mixes, and namely obtains the compound formulation of control grice diarrhoea (oral): attapulgite clay micropowders 88.5%, organic acid 3%, probio 3.5%, oligosaccharides 1%, vitamin C 1.5% and sucrose 2.5%.Wherein the attapulgite clay micropowders is attapulgite clay ultramicro grinding to 600 order, and attapulgite clay used is the high-quality original soil that directly selecting and purchasing gets from natural crystal, and makes the content of attapulgite be not less than 85% by purification processes; Organic acid used comprises 50% citric acid, 30% fumaric acid and 20% lactic acid by the quality percentage composition; Probio used is by the quality percentage composition, comprise bacillus 80% and lactic acid bacteria 20%, wherein bacillus is comprised of 50% bafillus natto, 40% bacillus subtilis and 10% bacillus licheniformis, bafillus natto by Yixing City section support one's family thing Co., Ltd provide (viable bacteria content〉1.6 * 10
10CFU/g), bacillus subtilis by nine divisions of China in remote antiquity, Nanjing interconnected agriculture and animal husbandry Science and Technology Ltd. provide (viable bacteria content〉1 * 10
10CFU/g), bacillus licheniformis by the U.S. refined come large pharmaceutical factory provide (commodity benefit poultry by name is precious, viable bacteria content〉2 * 10
10CFU/g), lactic acid bacteria (viable bacteria content〉3 * 10
10CFU/g) be that 10 times of the lactic acid bacteria product of inferior core (Nanjing) bio tech ltd dilutions are got; Oligosaccharides used comprises 50% xylo-oligosaccharide and 50% chitosan oligomer by the quality percentage composition.
Embodiment 3: the compound formulation animal experiment of control grice diarrhoea (drinking-water)
To carry out the weanling pig contrast test by the compound formulation of the control grice diarrhoea (drinking-water) of embodiment 1 method preparation.With same kind, close batch, 116 28 age in days weanling pigs (approximately 6.7 kilograms of average weights) that body weight is close, be divided at random 2 groups, be transferred to respectively two nursery houses that the position is adjacent, facility is identical, environment is consistent, wherein control group is 54, drinking-water management routinely; Remaining 62 is test group, by adding 150 gram compound formulations in every 100 liters of drinking-water, drinks continuously after on-test 5 days, drinks 1 day in 6-11 days every 2 days, drinks 1 day in every 3 days after the 11st day.Need stir when adding compound formulation in the test group drinking-water system, not precipitate for guaranteeing product suspension composition, after the interpolation compound formulation, stir 2-3 every day.Before test, pig house is comprehensively cleaned, sterilizes; Every day the timing feeding feed, feed adopts pig farm commodity baby pig feedstuff used; Freely drink water, other daily managements are carried out routinely; Duration of test carries out feeding and management by same people to two pig houses; Except drinking-water was processed, other processing between two groups of piglets was all identical.Test was carried out 21 days altogether, and every day, record was suffered from diarrhoea and a dead number.Calculate grice diarrhoea rate and the death rate during off-test: diarrhea rate=diarrhoea head/(for total number of examination pig * test number of days) * 100%; A piglet number * 100% when a piglet dead number/experimental period begins in the death rate=experimental period.The result of the test demonstration, the diarrhea rate of control group piglet and the death rate are respectively 13.4% and 9.3%; The diarrhea rate of test group piglet and the death rate are respectively 7.8% and 4.8%; Simultaneously, test group piglet spirit and the uniformity are obviously good than control group.As seen, this compound formulation product can obviously be prevented and treated grice diarrhoea, reduces the piglet death rate.
Embodiment 4: the compound formulation animal experiment of control grice diarrhoea (oral)
Certain pig farm finds that soon approximately symptom of diarrhea appears in the piglet of half after changing 62 same kinds, same batch, 28 age in days weanling pigs that body weight is close over to nursery house, and in 2 days dead 5 piglets.57 piglets of survival are divided into two groups, and wherein 18 piglets are control group; Remaining 39 piglets are test group.It is 3% solution that the compound formulation (method preparation as described in Example 2) that will prevent and treat grice diarrhoea (oral) is diluted to mass fraction, gives the test group Piglet by Oral, every day 2 times, each every approximately 250 milliliters, for three days on end, oral 1 time of the 4th～6 every day, do not use the compound formulation product on the 7th day; Control group Piglet by Oral equivalent drinking water.Two groups of piglets are at same pig house, and feeds utilized and feeding and management is all carried out routinely, and test was carried out 7 days altogether, and every day, record was suffered from diarrhoea and a dead number.Calculate grice diarrhoea rate and the death rate in 7 days during off-test: diarrhea rate=diarrhoea head/(for total number of examination pig * test number of days) * 100%; A piglet number * 100% when a piglet dead number/experimental period begins in the death rate=experimental period.The result of the test demonstration, the diarrhea rate of control group piglet and the death rate are respectively 38.9% and 22.2%; The diarrhea rate of test group piglet and the death rate are respectively 17.6% and 7.7%.As seen, this compound formulation product can obviously be prevented and treated grice diarrhoea, improves the piglet survival rate.
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| CN102488117A (en) | 2012-06-13 |
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