CN102488117A - Combination used for preventing and treating newborn pig diarrhea and preparation method and application thereof - Google Patents
Combination used for preventing and treating newborn pig diarrhea and preparation method and application thereof Download PDFInfo
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- CN102488117A CN102488117A CN2011104367798A CN201110436779A CN102488117A CN 102488117 A CN102488117 A CN 102488117A CN 2011104367798 A CN2011104367798 A CN 2011104367798A CN 201110436779 A CN201110436779 A CN 201110436779A CN 102488117 A CN102488117 A CN 102488117A
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- attapulgite clay
- grice diarrhoea
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Abstract
The invention discloses a combination used for preventing and treating newborn piglet diarrhea and a preparation method and application thereof. In percent by weight, the product comprises 88-98% of attapulgite ultra-fine powder, 0.1-3% of organic acid, 0.1-4% of probiotic, 0.05-2% of oligosaccharide, 0.05-1.5% of vitamin C and 0.15-3% of crane sugar. The combination disclosed by the invention is diluted into solution with the mass fraction of 0.05-3% with drinking water, the solution is used for feeding a piglet in an oral or drinking water manner, and the newborn piglet diarrhea caused by multiple factors can be effectively prevented and treated, thus the combination has the effects of improving immunocompetence of the piglet, improving antioxidant function, regulating intestinal microecology and protecting intestinal shape and can improve survival rate of the piglet, improve production performance of the piglet and relieve weaned stress on the piglet. The combination disclosed by the invention also has the characteristics of simple and convenient application mode, high efficiency, available raw materials, simple preparation and the like.
Description
Technical field
The invention belongs to the animal health field, be specifically related to a kind of compound formulation of preventing and treating grice diarrhoea.
Background technology
China is the big country of raising pigs, and the pig-breeding amount ranks first in the world.State Statistics Bureau's publish data shows, 2009-2010, and national live pig is delivered 64,570 ten thousand and 66,700 ten thousand respectively for sale, and still, China's Swine Production level is lower, exists problems such as the piglet death rate is high, the unit sow yields poorly.According to statistics, the nest that China raises pigs is all lived young several 9～9.5, and every sow year is produced 16～17 of big porkers; And the nest of culturing developed country is all lived young several 10～12, and every sow year is produced 20～22 of big porkers; It is 4,729 ten thousand that China sow in 2010 is raised a number, if by dead 3 calculating in every sow litter, then the annual dead piglet of China is about 14,200 ten thousand.Compare with developed country breed level, the main gap of China's pig industry is that the piglet death rate is high, and wherein diarrhoea is to cause main causes of death.Grice diarrhoea is a kind of typical multifactor property disease under the intensive pig production working condition; Can cause that piglet is dead, piglet survival ratio reduces, the price of deed descends, late growing stage slowly or stagnations of growing, become one of the pig industry development key factor that restricts.It is reported that the piglet death rate of China is generally 11%～25%, the death that the piglet that dies of illness causes for the diarrhoea relevant disease more than 80%.The grice diarrhoea priming factors mainly comprises infectiousness cause of disease and the non-infective disease cause of disease two aspects: in causing the infectiousness cause of disease of grice diarrhoea; Virus mainly contains Porcine epidemic diarrhea virus, transmissible gastro-enteritis virus and rotavirus, and bacterium mainly contains shigella dysenteriae, comma bacillus, Escherichia coli, salmonella and clostridieum welchii etc.; Non-infectious factor comprises mainly that stressors, piglet digestive function such as wean are complete, daily ration antigen is irritated, protein Excessive Intake and some trophic factors shortage etc.
At present; The control grice diarrhoea generally adopt antibiotic or other medicines carry out oral, drinking-water or the injection; Or add in feed that medicine prevents, treatment and alleviating diarrhoea; But the long-term use of antibiotic etc can make pathogenic microorganism produce drug resistance, and drug resistant gene can transmit mutually or shift between the bacterium of human, the animal and the ecosystem, causes animal and human's class catch back treatment difficulty even failure; In addition, the too much grievous injury piglet of drug use allelotaxis causes immunosupress, thereby influences piglet late growing stage and immunologic function; Moreover a large amount of uses of medicine will cause livestock products medicament residue problem, have a strong impact on food security, threaten human health.Therefore, the forbidding antibiotic has become certainty in the feed, presses for exploitation antibiotic substitute or other technical measures to prevent and treat Animal diseases.Recent year; The research of relevant antibiotic substitute in preventing and treating Animal diseases is a lot; Mainly contain Chinese herbal medicine or its extract, organic acid, oligosaccharides, probio and other organic-inorganic antiseptic etc.; These additives all have certain antibiotic or antivirus action, have the effect of control grice diarrhoea to a certain extent, have higher security.But when being applied to prevent and treat grice diarrhoea, multiple feed addictive all has some problems: because the physilogical characteristics of early weaning piglet and Chinese herbal medicine drug effect are slow and dosage is bigger than normal, palatability is poor; Aspects such as Chinese herbal medicine effective ingredients, active ingredient interphase interaction, drug effect, quality control and mechanism of action also lack the quantizating index and the further investigation of modern science, have limited the Chinese herbal medicine application in the weanling pig in early days; Organic acid makes an addition to can suppress the pathogenic microorganism growth in the piglet diet; But its action effect receives daily ration component, piglet age in days, kinds of organic acids and influence of various factors such as addition, feeding and management; Make the effect of organic acid in the control grice diarrhoea unstable; In use exist to discharge too fast, addition is big and problem such as cost height, simultaneously, make an addition to also to exist in the feed and destroy part nutrient stability in the feed, be prone to problems such as moisture absorption caking, etching mechanism; Use probiotics scalable alimentary canal microbial balances such as oligosaccharides and probio; But pathogenic entero becteria, normal flora and animal body Immune Effects are still waiting further research; Simultaneously; Such feed addictive only has certain preventive and therapeutic effect to bacillary with irritability diarrhoea, and virus diarrhea is not had direct effect; Inorganic antiseptic high doses such as copper, zinc or arsenic preparation pollute the environment when using, and also have residue problem, only have bacteriostasis simultaneously, and virus is not had direct prevention effect.When these novel fodder additives add in the feed feeding animals to; Because characteristics such as the feed digestion time are long in the animal, influence factor is more, make it to have utilization ratio lower, take effect comparatively slowly, action function is single and problem such as action effect instability.
Summary of the invention
Deficiency and the blank that remedies the market Related product to control grice diarrhoea prior art the object of the present invention is to provide a kind of compound formulation of preventing and treating grice diarrhoea.This compound formulation administered through oral or the water way piglet of feeding can prevent and treat grice diarrhoea effectively, and has the piglet of improvement production performance, alleviates action functions such as ablactation stress.
Another object of the present invention is to provide a kind of preparation method who prevents and treats the compound formulation of grice diarrhoea.
Another purpose of the present invention is to provide the application of above-mentioned compound formulation in the control grice diarrhoea.
Technical scheme of the present invention can realize through following technical scheme:
A kind of compound formulation of preventing and treating grice diarrhoea; By the quality percentage composition, comprise attapulgite clay micropowders 88%～98%, organic acid 0.1%～3%, probio 0.1%～4%, oligosaccharides 0.05%～2%, vitamin C 0.05%～1.5% and sucrose 0.15%～3%.
The compound formulation of above-mentioned control grice diarrhoea; It is that described attapulgite clay micropowders is more than attapulgite clay ultramicro grinding to 600 order; Described attapulgite clay also can be the modified product through Overheating Treatment, acid treatment, sodium salt processing, chitosan treatment or magnetic processing for the original soil that directly selecting and purchasing gets from natural crystal.
The compound formulation of above-mentioned control grice diarrhoea, its quality percentage composition that is attapulgite in the said attapulgite clay is not less than 60%; The quality percentage composition that is preferably attapulgite in the said attapulgite clay is not less than 85%.
The compound formulation of above-mentioned control grice diarrhoea, it is that described organic acid is a kind of or combination in any in citric acid, fumaric acid and the lactic acid.Described oligosaccharides is a kind of or combination in any in xylo-oligosaccharide and the chitosan oligomer.
The compound formulation of above-mentioned control grice diarrhoea, it is that by the quality percentage composition described probio comprises bacillus 50%～80% and lactic acid bacteria 20%～50%.
The compound formulation of above-mentioned control grice diarrhoea, it is described bacillus living content＞1 * 10
10CFU/g, described viable lactic acid bacteria content＞3 * 10
10CFU/g.
The compound formulation of above-mentioned control grice diarrhoea, it is that described bacillus is a kind of or combination in any in bacillus licheniformis, bafillus natto, the bacillus subtilis.
The compound formulation of above-mentioned control grice diarrhoea, its preparation method fully mixes organic acid, probio, oligosaccharides, vitamin C, sucrose and attapulgite clay micropowders for by described content proportioning.
The preparation method of the compound formulation of above-mentioned control grice diarrhoea; By described content proportioning; Organic acid, probio, oligosaccharides, vitamin C, sucrose and attapulgite clay micropowders are fully mixed, promptly get the compound formulation product of preventing and treating grice diarrhoea.
Above-mentioned compound formulation is used in the control grice diarrhoea.During use, using clean drinking water that the compound formulation product is diluted to mass fraction is 0.05%～3% solution, stirs back administered through oral or the water way piglet of feeding; Also can compound formulation be mixed the piglet of in feed, feeding.
Enteron aisle is the organ of digesting and assimilating of animal maximum; Also be immunity and endocrine organ maximum in the body; As contact external microbe and the maximum position of other harmful substance; Enteron aisle is the main door of pathogen invasion and nuisance invasion and attack, is again the first road barrier that stops cause pathogeny imcrobe infection and nuisance to pollute.The bacterium, virus and secreted toxin, ammonia or the harmful substances such as organic amino compounds, heavy metal that get into animal alimentary canal will be damaged intestinal mucosa and the epithelial cell of animal, influence disease resistance of animals, cause the multiple disease that comprises diarrhoea.In addition, animal in wean, change in the processes such as crowd, high temperature and inoculation, will produce various stress; Weanling pig particularly; Normal being prone to suffered from diarrhoea, and this mainly is due to the impaired and initiation pathogenic infection of the enteron aisle that stress cause, therefore; Take effective measures the protection enteron aisle, can effectively prevent grice diarrhoea.
Among the present invention, described attapulgite clay micropowders is more than attapulgite clay ultramicro grinding to 600 order, and the fine particle of formation is main with the particle less than 20 μ m particle diameters.The essential mineral composition of attapulgite clay is an attapulgite, be a kind of have a layer chain crystal structure contain Shuifu County's zeopan mineral, on mineralogy, be under the jurisdiction of sepiolite group.The structure of attapulgite can be divided into 3 layers, and two-layer up and down is the Si-O tetrahedral structure, and the centre is one deck Mg-O octahedral structure, and these construction units are staggered by the grid form, form bar-shaped, filamentary structure.Based on its particular shape structure, bigger specific area, special ionic forms; Make attapulgite clay have characteristics such as good selection absorption, cation exchange, load, suspension, rheological characteristic, heat endurance, all be widely used in each fields such as environmental protection, medicine, agricultural and chemical industry.Ground such as China Jiangsu, Gansu, Anhui abound with attapulgite clay, are the whole world the abundantest countries of attapulgite clay resource.Attapulgite clay has very strong covering power to gastrointestinal mucosal after getting into animal intestines and stomach; Has powerful adsorptivity, adsorbable various viruses, the bacterium interior and the toxin that is produced thereof, and adsorbable ammonia or harmful substances such as organic amino compounds, heavy metal ion with suppressing animal gastrointestinal tract; Guarantee to have good physiological environment in the alimentary canal; Promote the reparation of barrier of gastric mucosa, regulate the gut flora balance, thus protection enteron aisle and mucous membrane function.It is reported that attapulgite clay or its partially modified product can adsorb microorganisms such as Escherichia coli, salmonella, can effectively adsorb the toxin in the enteron aisle, like cholera toxin and ECT.In addition, attapulgite clay has very strong water imbibition, and its contained exchangeable cations also can be animal several kinds of mineral elements such as magnesium, iron, zinc are provided, and has the effect that promotes metabolism, strengthens health.Therefore, the attapulgite clay product has good enteron aisle and mucous membrane protectiveness, and the diarrhoea that the Different types of etiopathogenises that animal is taken place causes all has regulating and controlling effect preferably.In American-European countries; The at present existing multiple pharmaceutical preparation that is main component with attapulgite or its like product montmorillonite; Be mainly used in treatment diarrhoea and gastrointestinal disease etc.; Main component as at the fast-selling good Smecta of disease effects (dioctahedral smectite) products such as control diarrhoea of China is montmorillonite, but its price is more expensive and need import.Hospital of China PLA is that primary raw material is developed the novel enteron mucosa protective agent with domestic resourceful attapulgite clay; Clinical trial is the result show; It can be used as one of active drug of treatment diarrhoea; Gastrointestinal mucosal is had the significant protection effect, also meets six standards of anti-diarrheal that WHO formulates, promptly efficient, orally-ingestible, can with the ORT compatibility with, not by intestinal absorption, do not influence intestinal absorption ability and can resist a series of enteron aisle cause of diseases.Repeatedly animal experiment according to the inventor shows that attapulgite clay has the certain protection effect to animal intestinal form and health.In addition, attapulgite clay has good suspension, can in the aqueous solution, form the suspension of good stability, can be made into oral or the drinking-water product; Attapulgite clay has load-carrying properties and powerful bearing capacity preferably, and has good rheological characteristic, can be used as the carrier of multiple feed addictive; After adopting superfine communication technique that attapulgite clay is processed micropowders, can improve its area coverage, absorption property and suspendability.Therefore, among the present invention with the superfine powder of attapulgite clay original soil or its modified product as main component, be mixed with piglet patent intestinal protection, administered through oral or the water way piglet of feeding is with the control grice diarrhoea.Attapulgite clay described in the present invention also can be the modified product through Overheating Treatment, acid treatment, sodium salt processing, chitosan treatment, magnetic processing for the high-quality original soil that directly selecting and purchasing gets from natural crystal; Wherein the content of attapulgite is not less than 60% in the attapulgite clay, is preferably through purification processes to make the content of attapulgite be not less than 85%.
Among the present invention, described organic acid is a kind of or combination in any in citric acid, fumaric acid and the lactic acid.Organic acid is mainly used in young animal; Its main purpose is for the replenishing gastric acid deficiency; Reduce enteron aisle pH value, to the secretion that suppresses pathogenic microorganism growth, the enzyme that stimulates digestion, promote to digest and improve aspect such as intestinal environment and all have positive meaning, can prevent the generation of diseases such as suffering from diarrhoea; Simultaneously, also have the palatability of improvement, ptyalize, promote effects such as trace elements absorbed and utilization.Organic acid commonly used now has citric acid, fumaric acid, lactic acid, malic acid and fumaric acid etc.Organic acid not only can be applicable in the feed, and research shows, in drinking-water, adds organic acid and can kill the bacterium in drinking-water system and the alimentary canal, improves the digestibility of protein, and regulates the microbiota in the enteron aisle.Citric acid is a kind of organic acid that has than the strong acid flavor, is the synergist of anti-oxidant; Fumaric acid is a kind of sour unsaturated dicarboxylic acid, in water, has dissolubility preferably, except that possessing the organic acid functional characteristic, also has non-oxidizability and the effect of strengthening vitamin stability; Lactic acid is a kind of organic acid with particular tastes, can be used as anticorrisive agent and uses, it is reported, lactic acid can suppress harmful bacterium such as Escherichia coli and salmonella in the intestine of young pigs growth, improve the piglet production performance.Among the present invention through organic acid self function and with other composition between synergy, can prevent and reduce grice diarrhoea, improve digestion and absorption function, alleviate stress, thereby raising piglet production performance.The present invention uses organic acid to process oral or the drinking-water product; Overcome organic acid and in feed, added influencing part nutrient stability, being prone to shortcomings such as moisture absorption caking, etching mechanism of being produced; Simultaneously, by means of the absorption and the bearing function of attapulgite clay, organic acid is slowly discharged in alimentary canal; Make its effect last till the enteron aisle back segment always, play good acidifying and bacteriostasis effect.
Research shows that probio is introduced alimentary canal as useful inoculating microbe, on value volume and range of product, replenishes the normal microorganism that reduces or lack in the enteron aisle; With harmful bacteria competition ecological niche; And can produce antibacterial material, and suppressing the growth and breeding of pathogenic bacteria, adjustment recovers the colony balance of enteron aisle; Add a certain amount of probio in feed or the drinking-water, all have certain effect improving immunity, control grice diarrhoea, alleviate ablactation stress and improve aspects such as piglet survival ratio.Use the compound probiotic that comprises bacillus and lactic acid bacteria among the present invention, can bring into play the synergy of many bacterial classifications.Characteristics such as that functional oligosaccharide has is stable, safety non-toxic; It can not can be combined with pathogen by the digestive ferment of animal digestion itself, the field planting in animal body of blocking-up pathogenic microorganism; Can be in enteron aisle as the substrate of beneficial bacteriums such as Bifidobacterium; Promote the propagation of beneficial bacterium, the harmful bacteria growing of inhibition simultaneously uses oligosaccharides can improve immune performance in animal produces, reduces effects such as the death rate and promotion growth performance.The present invention unites use probio and oligosaccharides, and the double action that is intended to bring into play simultaneously both is through reducing the field planting of pathogenic microorganism; Promote the field planting in animal intestinal of exogenous viable bacteria; Optionally stimulate the growth and the breeding of one or more beneficial bacteriums, improve the formation of microbial population of animal intestinal tract, regulate the little ecology of alimentary canal; Improve immunocompetence and guarantee host health, thereby effectively prevent grice diarrhoea.Probio described in the present invention by the quality percentage composition, comprises bacillus 50%～80% and lactic acid bacteria 20%～50%, and wherein bacillus is a kind of or combination in any in bacillus licheniformis, bafillus natto, the bacillus subtilis.Oligosaccharides described in the present invention is a kind of or combination in any in xylo-oligosaccharide and the chitosan oligomer.
Vitamin C has another name called ascorbic acid, is a kind of anti-oxidant; Have very strong anti-radical action, have anti-stress ability preferably simultaneously, can be through the concentration of hypothalamic-pituitary-adrenal reaction axle adjusting stress hormone cortisol; Improve human body immune function, reach anti-stress purpose.It is reported that wean back piglet blood plasma level of vitamin C sharply descends, promptly ablactation stress makes piglet increase ascorbic requirement, and this moment, an amount of vitamin C that replenishes had tangible anti-stress effect.Vitamin C is a water soluble vitamin, and compound formulation product administered through oral of the present invention or drinking-water are fed to piglet, can improve ascorbic functioning efficiency.
The sense of taste of pig is relatively more responsive, in sour, sweet, bitter, peppery, salty 5 kinds of sense of taste, especially sweet taste is liked most.Sweetener has the modification masking ability to peculiar smell, can improve the animal sense of taste, promotes digesting and assimilating and utilizing of feed, improves feed intake and amount of drinking water, especially young animal and shows more obviously with the animal that is in stress situation.Discover that pig has strong preference to the sucrose glassware for drinking water; Distinguish the flavor of near sucrose more when sweet taste, quality is good more, and pig is just liked more; The cane-sugar taste of molasses type is the favorite tastes of animal such as sucking pig, piglet and calf.It is sweetener that the present invention selects sucrose for use, improves the palatability of piglet to product.
Except that specified otherwise, raw material that the present invention relates to and reagent are all commercially available to be got.
Among the present invention; The preparation method of the compound formulation of described control grice diarrhoea; Be by aforesaid addition or content proportioning; Organic acid, probio, oligosaccharides, vitamin, sucrose and attapulgite clay micropowders are fully mixed, promptly get the compound formulation product of preventing and treating grice diarrhoea.
It is 0.05%～3% solution that the compound formulation of control grice diarrhoea of the present invention uses clean drinking water that it is diluted to mass fraction; Stir back administered through oral or the water way piglet of feeding; Perhaps compound formulation is mixed the piglet of in feed, feeding; Can effectively prevent and treat the grice diarrhoea that multiple factor causes, improve the piglet survival rate.
Beneficial effect of the present invention is:
1, according to the piglet physilogical characteristics and cause the reason of diarrhoea; Based on characteristics such as the enteron aisle defencive function of attapulgite clay and bearing capacitys; Adopt multi-functional, multi-level technical measures, combine multiple single feed additive to carry out integrated assembling the inventor's technological innovation (attapulgite clay superfine communication technique), a kind of compound formulation of preventing and treating grice diarrhoea is provided; The piglet but administered through oral or water way are fed has been filled up the blank of market similar action product.
2, with the attapulgite clay micropowders be the compound formulation of the control grice diarrhoea of main component; After administered through oral or water way get into the piglet stomach and intestine; Gastrointestinal mucosal is had very strong covering power, can improve the defense function of mucous membrane barrier, keep gastral normal physiological function then; Simultaneously can effectively adsorb and remove bacterium, virus and other harmful substance of causing a disease; And have the multiple bioactive functions of multiple novel fodder additive, not only effective to bacterial diarrhea, the diarrhoea that stressors such as viral and wean are caused also has tangible prevention effect.
3, this compound formulation product has raising piglet immunological ability, improves anti-oxidation function, regulates the effect of intestinal microecology and protection enteron aisle form, can improve the piglet survival rate, improves the piglet production performance, improves piglet health, alleviates stress of baby pigs caused ablaction etc.
4, add to like product and be applied in the feed compare in the piglet; The piglet but this compound formulation product administered through oral or drinking-water are fed; Application mode is easy; Have more directly, effect efficiently, simultaneously because carrying, absorption and the slow releasing function of attapulgite clay can make organic acid isoreactivity material play more efficient and permanent effect.
5, as the attapulgite clay aboundresources of product main component, the product compound method is prone to row, and is easy and simple to handle, applicable to large-scale production and application.
The specific embodiment
Below in conjunction with embodiment the present invention is described further, but the present invention is not limited.
Embodiment 1: the preparation of the compound formulation of control grice diarrhoea (drinking-water)
With the compound formulation quality of control grice diarrhoea of the present invention by 100 parts; Carry out proportioning and fully mix by the ratio of quality and the number of copies that is described below, promptly obtain the compound formulation of control grice diarrhoea (drinking-water): attapulgite clay micropowders 95%, organic acid 1%, probio 1.2%, oligosaccharides 0.5%, vitamin C 0.8% and sucrose 1.5%.Wherein the attapulgite clay micropowders is attapulgite clay ultramicro grinding to 600 order, and used attapulgite clay is the high-quality original soil that directly selecting and purchasing gets from natural crystal, and the content of contained attapulgite is not less than 65%; Used organic acid comprises 70% citric acid and 30% fumaric acid by the quality percentage composition; Used probio is by the quality percentage composition; Comprise bacillus 70% and lactic acid bacteria 30%; Wherein bacillus is made up of 50% bafillus natto, 40% bacillus subtilis and 10% bacillus licheniformis, and bafillus natto provides (viable bacteria content＞1.6 * 10 by the thing Co., Ltd that supports one's family of Yixing City section
10CFU/g), bacillus subtilis provides (viable bacteria content＞1 * 10 by the interconnected agriculture and animal husbandry in nine divisions of China in remote antiquity, Nanjing Science and Technology Ltd.
10CFU/g), bacillus licheniformis provides (commodity benefit poultry by name treasured, viable bacteria content＞2 * 10 by the refined next big pharmaceutical factory of the U.S.
10CFU/g), lactic acid bacteria (viable bacteria content＞3 * 10
10CFU/g) be that 10 times of the lactic acid bacteria product of inferior core (Nanjing) bio tech ltd dilutions are got; Used oligosaccharides is an xylo-oligosaccharide.
Embodiment 2: the preparation of the compound formulation of control grice diarrhoea (oral)
With the compound formulation quality of control grice diarrhoea of the present invention by 100 parts; Carry out proportioning and fully mix by the ratio of quality and the number of copies that is described below, promptly obtain the compound formulation of control grice diarrhoea (oral): attapulgite clay micropowders 88.5%, organic acid 3%, probio 3.5%, oligosaccharides 1%, vitamin C 1.5% and sucrose 2.5%.Wherein the attapulgite clay micropowders is attapulgite clay ultramicro grinding to 600 order, and used attapulgite clay is the high-quality original soil that directly selecting and purchasing gets from natural crystal, and makes the content of attapulgite be not less than 85% through purification processes; Used organic acid comprises 50% citric acid, 30% fumaric acid and 20% lactic acid by the quality percentage composition; Used probio is by the quality percentage composition; Comprise bacillus 80% and lactic acid bacteria 20%; Wherein bacillus is made up of 50% bafillus natto, 40% bacillus subtilis and 10% bacillus licheniformis, and bafillus natto provides (viable bacteria content＞1.6 * 10 by the thing Co., Ltd that supports one's family of Yixing City section
10CFU/g), bacillus subtilis provides (viable bacteria content＞1 * 10 by the interconnected agriculture and animal husbandry in nine divisions of China in remote antiquity, Nanjing Science and Technology Ltd.
10CFU/g), bacillus licheniformis provides (commodity benefit poultry by name treasured, viable bacteria content＞2 * 10 by the refined next big pharmaceutical factory of the U.S.
10CFU/g), lactic acid bacteria (viable bacteria content＞3 * 10
10CFU/g) be that 10 times of the lactic acid bacteria product of inferior core (Nanjing) bio tech ltd dilutions are got; Used oligosaccharides comprises 50% xylo-oligosaccharide and 50% chitosan oligomer by the quality percentage composition.
Embodiment 3: the compound formulation animal experiment of control grice diarrhoea (drinking-water)
To carry out the weanling pig contrast test by the compound formulation of the control grice diarrhoea (drinking-water) of embodiment 1 method preparation.With same kind, close batch, 116 28 age in days weanling pigs (about 6.7 kilograms of average weight) that body weight is close; Be divided into 2 groups at random; Be transferred to two child care houses that the position is adjacent, facility is identical, environment is consistent respectively, wherein control group is 54, by the management of routine drinking-water; Remaining 62 is test group, by adding 150 gram compound formulations in per 100 liters of drinking-water, drinks continuously after on-test 5 days, drinks 1 day in 6-11 days per 2 days, drinks 1 day in per 3 days after the 11st day.Need stir when adding compound formulation in the test group drinking-water system, not precipitate for guaranteeing product suspension composition, stir 2-3 every day behind the interpolation compound formulation.Before the test pig house is comprehensively cleaned, sterilizes; Every day is feeding feed stuff regularly, and feed adopts the used commodity baby pig feedstuff in pig farm; Freely drink water, other daily managements are undertaken by routine; Duration of test carries out feeding and management by same people to two pig houses; Except that drinking-water was handled, other processing between two groups of piglets was all identical.Test was carried out 21 days altogether, and every day, record was suffered from diarrhoea and a dead number.Calculate the grice diarrhoea rate and the death rate during off-test: diarrhea rate=diarrhoea head/(supplying total number of examination pig * test fate) * 100%; A piglet number * 100% when a piglet dead number/experimental period begins in the death rate=experimental period.Result of the test shows that the diarrhea rate of control group piglet and the death rate are respectively 13.4% and 9.3%; The diarrhea rate of test group piglet and the death rate are respectively 7.8% and 4.8%; Simultaneously, the test group piglet spirit and the uniformity are obviously good than control group.It is thus clear that this compound formulation product can obviously be prevented and treated grice diarrhoea, reduce the piglet death rate.
Embodiment 4: the compound formulation animal experiment of control grice diarrhoea (oral)
Certain pig farm finds that soon symptom of diarrhea appears in half the approximately piglet after changing 62 same kinds, same batch, 28 age in days weanling pigs that body weight is close over to the child care house, and in 2 days dead 5 piglets.57 piglets of survival are divided into two groups, and wherein 18 piglets are control group; Remaining 39 piglets are test group.It is 3% solution that the compound formulation (shown in embodiment 2 method preparation) that will prevent and treat grice diarrhoea (oral) is diluted to mass fraction, gives the test group piglet oral, every day 2 times; Each every about 250 milliliters; For three days on end, oral 1 time of the 4th～6 every day, do not use the compound formulation product on the 7th day; The oral equivalent drinking water of control group piglet.Two groups of piglets are at same pig house, and feeds utilized and feeding and management is all undertaken by routine, and test was carried out 7 days altogether, and every day, record was suffered from diarrhoea and a dead number.Calculate the grice diarrhoea rate and the death rate in 7 days during off-test: diarrhea rate=diarrhoea head/(supplying total number of examination pig * test fate) * 100%; A piglet number * 100% when a piglet dead number/experimental period begins in the death rate=experimental period.Result of the test shows that the diarrhea rate of control group piglet and the death rate are respectively 38.9% and 22.2%; The diarrhea rate of test group piglet and the death rate are respectively 17.6% and 7.7%.It is thus clear that this compound formulation product can obviously be prevented and treated grice diarrhoea, improve the piglet survival rate.
Claims (10)
1. compound formulation of preventing and treating grice diarrhoea; It is characterized in that by the quality percentage composition, comprise attapulgite clay micropowders 88%～98%, organic acid 0.1%～3%, probio 0.1%～4%, oligosaccharides 0.05%～2%, vitamin C 0.05%～1.5% and sucrose 0.15%～3%.
2. the compound formulation of control grice diarrhoea according to claim 1; It is characterized in that described attapulgite clay micropowders is more than attapulgite clay ultramicro grinding to 600 order; Described attapulgite clay also can be the modified product through Overheating Treatment, acid treatment, sodium salt processing, chitosan treatment or magnetic processing for the original soil that directly selecting and purchasing gets from natural crystal.
3. the compound formulation of control grice diarrhoea according to claim 2 is characterized in that the quality percentage composition of attapulgite in the said attapulgite clay is not less than 60%; The quality percentage composition that is preferably attapulgite in the said attapulgite clay is not less than 85%.
4. the compound formulation of control grice diarrhoea according to claim 1 is characterized in that described organic acid is a kind of or combination in any in citric acid, fumaric acid and the lactic acid; Described oligosaccharides is a kind of or combination in any in xylo-oligosaccharide and the chitosan oligomer.
5. the compound formulation of control grice diarrhoea according to claim 1 is characterized in that by the quality percentage composition, and described probio comprises bacillus 50%～80% and lactic acid bacteria 20%～50%.
6. the compound formulation of control grice diarrhoea according to claim 5 is characterized in that described bacillus living content＞1 * 10
10CFU/g, described viable lactic acid bacteria content＞3 * 10
10CFU/g.
7. according to the compound formulation of claim 5 or 6 described control grice diarrhoeas, it is characterized in that described bacillus is a kind of or combination in any in bacillus licheniformis, bafillus natto, the bacillus subtilis.
8. the compound formulation of control grice diarrhoea according to claim 1; It is characterized in that its preparation method is by the described content proportioning of claim 1, fully mixes organic acid, probio, oligosaccharides, vitamin C, sucrose and attapulgite clay micropowders.
9. the preparation method of the described control grice diarrhoea of claim 1 compound formulation is characterized in that by the described content proportioning of claim 1 organic acid, probio, oligosaccharides, vitamin C, sucrose and attapulgite clay micropowders fully being mixed.
10. the described compound formulation of claim 1 is used in the control grice diarrhoea.
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