CN104758318A - A composite preparation for reducing a diarrhea rate of weaned piglets and a preparing method thereof - Google Patents

A composite preparation for reducing a diarrhea rate of weaned piglets and a preparing method thereof Download PDF

Info

Publication number
CN104758318A
CN104758318A CN201510102291.XA CN201510102291A CN104758318A CN 104758318 A CN104758318 A CN 104758318A CN 201510102291 A CN201510102291 A CN 201510102291A CN 104758318 A CN104758318 A CN 104758318A
Authority
CN
China
Prior art keywords
liquid
acid
diarrhea
acidulant
weaned piglets
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201510102291.XA
Other languages
Chinese (zh)
Other versions
CN104758318B (en
Inventor
单宝龙
陈雷
谷巍
王红
孙明杰
林显华
崔斌
王军
徐鹏
董佩佩
王春凤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHANDONG BAOLAI-LEELAI BIO-ENGINEERING Co Ltd
Original Assignee
SHANDONG BAOLAI-LEELAI BIO-ENGINEERING Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHANDONG BAOLAI-LEELAI BIO-ENGINEERING Co Ltd filed Critical SHANDONG BAOLAI-LEELAI BIO-ENGINEERING Co Ltd
Priority to CN201510102291.XA priority Critical patent/CN104758318B/en
Publication of CN104758318A publication Critical patent/CN104758318A/en
Application granted granted Critical
Publication of CN104758318B publication Critical patent/CN104758318B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

A composite preparation for reducing a diarrhea rate of weaned piglets is disclosed. The composite preparation is prepared by mixing an oligosaccharide-acidifier solution and a probiotic fermentation liquor according to a volume ratio of 1:(3-5). Each mL of the oligosaccharide-acidifier solution contains 2500-3500 mug of oxalic acid, 30-60 mug of tartaric acid, 50-100 mug of malic acid, 4000-5500 mug of lactic acid, 2500-4000 mug of citric acid, 0.05-0.2 g of glucose, 0.05-0.1 g of isomaltooligosaccharide, 0.02-0.06 g of fructooligosaccharide and 0.08-0.15 g of chitosan oligosaccharide. The probiotic fermentation liquor is prepared by mixing a mixed fermentation liquor of lactobacillus plantarum and pediococcus pentosaceus and a clostridium butyricum fermentation liquor according to a volume ratio of (2-4):1. A preparing method of the composite preparation is also disclosed. The composite preparation reduces the diarrhea rate of weaned piglets, reduces the death rate of piglets, and boosts productive performance and immunity of piglets.

Description

A kind ofly reduce compound formulation of diarrhea of weaned piglets rate and preparation method thereof
Technical field
The present invention relates to and a kind ofly reduce compound formulation of diarrhea of weaned piglets rate and preparation method thereof.
Background technology
In recent years, China's pig industry development is very rapid, and management and the large-scale degree of cultivation are obtained for improvement.Along with the raising of Swine Production technical merit, early weaning piglet technology has progressively been popularized in the countries and regions of some pig industry prosperities, but crossing early-weaning can make piglet stress nutrition, environment and psychological generation, cause feed intake minimizing, growth retardation, weight loss, diarrhoea, even dead.It is reported, the piglet of below 30kg, annual diarrhea disease percentage 46.5%, mortality rate 10.3%.Therefore, how to prevent and treat diarrhea of weaned piglets and become one of urgent task of current pig industry.
Up to the present, domesticly in the control of diarrhea of weaned piglets disease adopt antibiotic etc direct injection or add in feedstuff, along with a large amount of appearance of Resistant strain, the existing chemicals of frequent discovery controls diarrhoea poor effect, and a large amount of uses of these medicines easily cause livestock products drug residue and in human body enrichment serious threat the health of the mankind; Vaccine immunity, though the antibody content that can improve in sow milk, the antibody persistent period is short, is not enough to protection piglet whole growth stage and does not suffer from diarrhoea, and e. coli serotype complicated, constantly change, even if immune piglet is produced effects also very micro-; Chinese herbal medicine drug effect is slow, and dosage is large, and palatability is poor, limits its application in ablactational baby pig daily ration; The trace element such as high-copper, Gao Xin is also applied to some extent, but easily residual, pollute large, easily cause heavy metal poisoning; Enzyme preparation also adopts to some extent, but application point is too single, cannot solve the diarrhoea caused due to alteration of intestinal flora.Therefore, finding one can effectively reduce diarrhea of weaned piglets rate, and the product of harm humans health is not imperative simultaneously.
Summary of the invention:
For the deficiency that above-mentioned prior art exists, the object of this invention is to provide and a kind ofly reduce compound formulation of diarrhea of weaned piglets rate and preparation method thereof, this compound formulation can reduce diarrhea of weaned piglets rate, reduces the mortality rate of piglet, improves fertility performance and the immunologic function of piglet.
For achieving the above object, the present invention adopts following technical scheme:
Reducing a compound formulation for diarrhea of weaned piglets rate, is by oligosaccharide acidulant liquid and probiotics fermention liquid 1:(3-5 by volume) mix;
Contain in the described oligosaccharide acidulant liquid of every 1mL:
Oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid 50-100 μ g, lactic acid 4000-5500 μ g, citric acid 2500-4000 μ g, glucose 0.05-0.2g, oligomeric isomaltose 0.05-0.1g, oligofructose 0.02-0.06g, oligo-chitosan 0.08-0.15g;
Preferably, contain in the described oligosaccharide acidulant liquid of every 1mL: oxalic acid 3000 μ g, tartaric acid 50 μ g, malic acid 60 μ g, lactic acid 5000 μ g, citric acid 3000 μ g, glucose 0.1g, oligomeric isomaltose 0.05g, oligofructose 0.05g, oligo-chitosan 0.1g.
Preferably, described oligo-chitosan is polymerized by 2-10 glycosidic bond.
Described probiotics fermention liquid is by Lactobacillus plantarum, Pediococcus pentosaceus mixed culture fermentation liquid and Clostridium butyricum fermentation liquid by volume (2-4): 1 mixes, and in this probiotics fermention liquid, the viable count of Lactobacillus plantarum is greater than or equal to 1 × 10 8cfu/mL, the viable count of Pediococcus pentosaceus is greater than or equal to 1 × 10 8cfu/mL, the viable count of Clostridium butyricum is greater than or equal to 1 × 10 7cfu/mL.
Preferably, described probiotics fermention liquid be by Lactobacillus plantarum, Pediococcus pentosaceus mixed culture fermentation liquid and Clostridium butyricum fermentation liquid by volume 2:1 mix.
Described Lactobacillus plantarum, Pediococcus pentosaceus mixed culture fermentation liquid are obtained by Lactobacillus plantarum LP (Lactobacillus plantarum), Pediococcus pentosaceus PP (Pediococcus pentosaceus PP) co-fermentation, this technology is documented in a patent of invention " a kind of lactobacillus semisolid fermentation product and technique improving growth of animal performance " (CN 102599348 B) of applicant, and fermentation process is with " concrete fermentation technology " step (1)-(4) in embodiment 3 in this patent; Wherein Pediococcus pentosaceus PP (Pediococcuspentosaceus PP) is preserved in China typical culture collection center, and its deposit number is CCTCC NO:M 2012029; Lactobacillus plantarum LP (Lactobacillus plantarum) is documented in another patent of invention of applicant " strain can be used for lactobacillus and the application thereof of biological preservation " (CN101914475 B), be preserved in China typical culture collection center, its deposit number is CCTCC M 2010150.
Described Clostridium butyricum fermentation liquid is fermented by Clostridium butyricum (Clostridium butyricum) Cb-2 to obtain, this Clostridium butyricum is documented in another patent of invention " strain has the Clostridium butyricum for the treatment of diarrhoea effect and the application as substitute of sodium butyrate thereof " (CN 102559550 B) of applicant, be preserved in China typical culture collection center, its deposit number is: CCTCC M2011384; Fermentation process is with step (i)-(v) of " 4.6 prepare Clostridium butyricum mycopowder " part in embodiment 1 in this patent ".
In the prescription of this compound formulation, oligosaccharide is small molecule saccharide, because it can not digested road endogenous enzymes digest, also can not be utilized by harmful bacteria, thus can promote that probiotics grows, breeding, thus balance intestinal flora, suppress the generation of diarrhoea.Probiotics fermention liquid has regulation and control intestinal microbial population balance, suppresses the breeding of the harmful bacteria such as escherichia coli, Salmonella, the effect of preventing post-weaning diarrhea.Acidulant refers to the monomer or mixture that can join organic acid in feedstuff and mineral acid, and its effect, mainly through improving gastrointestinal micro-flora, suppresses the growth of harmful bacteria, promotes the propagation of probiotics; Regulate stomach inner pH value and the intestine evacuation velocity slowing down stomach, thus promote digesting and assimilating of nutrient substance; Improve the palatability of feedstuff, appetite strengthening.Three is composite reduces diarrhea of weaned piglets rate.
The preparation method of this reduction diarrhea of weaned piglets rate compound formulation, step is as follows:
(1) by oxalic acid, tartaric acid, malic acid, lactic acid, citric acid, glucose, oligomeric isomaltose, oligofructose and oligo-chitosan mixing, use water as solvent, heat and stir, it is made to dissolve completely, cooling, adjustment pH is 3.0-5.0, obtained oligosaccharide acidulant liquid, contain in the oligosaccharide acidulant liquid of every 1mL: oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid 50-100 μ g, lactic acid 4000-5500 μ g, citric acid 2500-4000 μ g, glucose 0.05-0.2g, oligomeric isomaltose 0.05-0.1g, oligofructose 0.02-0.06g, oligo-chitosan 0.08-0.15g,
(2) by Lactobacillus plantarum LP, Pediococcus pentosaceus PP mixed culture fermentation liquid and Clostridium butyricum Cb-2 fermentation liquid by volume (2-4): 1 mixes, in this probiotics fermention liquid, the viable count of Lactobacillus plantarum LP, Pediococcus pentosaceus PP and Clostridium butyricum Cb-2 is greater than or equal to 1 × 10 respectively 8cfu/mL, 1 × 10 8cfu/mL and 1 × 10 7cfu/mL;
(3) the probiotics fermention liquid 1:(3-5 by volume that oligosaccharide acidulant liquid step (1) obtained and step (2) obtain) mixing, to obtain final product.
Preferably, in described step (3), the 1:4 mixing by volume of oligosaccharide acidulant liquid and probiotics fermention liquid.
Beneficial effect of the present invention:
(1) to adopt oligosaccharide, probiotic bacteria, acidulant to carry out first composite in the present invention, have developed the product that effectively can reduce diarrhea of weaned piglets rate; Wherein oligosaccharide, probiotic bacteria, acidulant three synergism, on the one hand, oligosaccharide is small-molecule substance, can be made full use of, and do not consumed by harmful bacteria, final balance intestinal flora by probiotic bacteria, reduces the probability of happening of diarrhoea; On the other hand, lactobacillus produces acid, reduces the quantity of pathogenic bacterium, and Clostridium butyricum Antidiarrheic effect is good, and both compounds can reduce the generation of diarrhoea; The third aspect, acidulant can reduce gut pH, promotes lactobacillus field planting, is conducive to producing enzyme, improves feed digestion utilization rate.
(2) to select Clostridium butyricum, Lactobacillus plantarum and Pediococcus pentosaceus to carry out composite in the present invention, and wherein Clostridium butyricum metabolism produces the organic acid such as butanoic acid, acetic acid, and bacteriostasis rate is high; Lactobacillus plantarum, Pediococcus pentosaceus are intestinal dominant microflora, can set up dominant microflora in intestinal; Oligomeric functional sugar simultaneously, can promote that probiotics grows, breeding, do not utilized by harmful bacteria, oligo-chitosan also has effect of similar antibiotic, by regulating intestinal canal microecological environment, plays the anti-diarrhea effect of growth promotion.
(3) evaluated the effect of compound formulation of the present invention by feces count plate and S-IgA assay, result proves that compound formulation of the present invention significantly can reduce the diarrhea rate of ablactational baby pig; Improve piglet average daily gain, average daily ingestion amount, significantly reduces feedstuff-meat ratio, improves the fertility performance of piglet; Lactic acid bacterium number in remarkable increase feces, reduces escherichia coli quantity, balance intestinal flora; Remarkable reduction ablactational baby pig fecal energy and crude fat content, improve efficiency of feed utilization; Significantly improve feces sIgA content, strengthen mucosa-immune ability.
Accompanying drawing explanation
Fig. 1 a: be matched group piglet feces diagram in embodiment 3, watery stools is more;
Fig. 1 b: be test group piglet feces diagram in embodiment 3 is feces molding, harder;
Fig. 1 c: be Related product group feces diagram in embodiment 3, feces is thin, soft stool.
Detailed description of the invention
The present invention is further illustrated in conjunction with the embodiments, should be noted that following explanation is only to explain the present invention, not limiting its content.
Embodiment 1: the compound acidulant screening test of control diarrhea of weaned piglets
1 materials and methods
1.1 EXPERIMENTAL DESIGN
Test is carried out in Ba Fubafu plant of drink township of Ningyang County of Shandong Province township, select the ablactational baby pig 90 that the same kind of 30-33 age in days, body weight are close, be divided into matched group (CK), test group I (F1) and test group II (F2), each group 3 repetitions, each repetition 10 pigs.Matched group every day, drinking-water was fed clear water, test group I to feed compound acidulant formula 1 (water dilutes 10 times) every day, test group II to drink water compound acidulant formula 2 (water dilutes 10 times) of feeding every day, feed 14d continuously, change clear water after feeding into normally to feed, observe 7d afterwards, examine pig diarrhoea situation.
Compound acidulant formula 1: every 1mL compound acidulant contains oxalic acid 3000 μ g, tartaric acid 50 μ g, malic acid 60 μ g, lactic acid 5000 μ g, citric acid 3000 μ g;
Compound acidulant formula 2: every 1mL compound acidulant contains oxalic acid 300 μ g, tartaric acid 100 μ g, lactic acid 1000 μ g, acetic acid 1000 μ g, citric acid 500 μ g.
1.2 test daily rations
Daily ration basic components is Semen Maydis 58%, bean cake 24%, wheat bran 3.8%, fish flour (import) 5%, whey powder 5%, vegetable oil 1%, stone powder 0.7%, CaHCO 31%, Sal 0.2%, L-lysine hydrochloride 0.3%, premix material 1%.Its trophic level is CP (crude protein) 9.8%, Ca 0.87%, P 0.05%, lysine 1.31% (being mass percent).
1.3 feeding and management
Feed every day 4 times, free choice feeding, supply clear water during control group fed, test group I, test group II compound acidulant dilute with water 10 times are drunk water afterwards and are fed.Every day sooner or later respectively sweeps pigsty and gives up 1 time, and every 7d cleaning and sterilization crib, apparatus 1 time, examine and search for food and feces situation of change.Test period is 21d, weighs on an empty stomach respectively at test 0d, 21d 9:00 in morning, calculates the average daily gain of test group I, test group II and matched group piglet, average daily ingestion amount, feedstuff-meat ratio etc.
1.4 diarrhea rates calculate
Record piglet to be suffered from diarrhoea head number every day, calculates:
Diarrhea rate=[this circle day diarrhoea piglet head number summation/(total head number × natural law of piglet)] × 100% piglet diarrhea degree evaluation foundation is in table 1.When occurring that mild diarrhea is feces normality on the same day, count secondary 1 time of diarrhoea head.
Table 1 Scours index evaluating basis
1.5 faecal microbiota countings and trophic level measure
During off-test, test group, matched group are got appropriate feces respectively and are taken back experimental determination faecal microbiota and trophic level (energy, crude protein, crude fat etc.).Faecal microbiota counting adopts gradient dilution method to carry out, and feces trophic level measures and adopts energy measuring device, crude protein, determinator for crude fats to measure.
1.6 data analysiss adopt SPSS software to carry out data analysis, and result represents with X ± S.
2 results and analysis
2.1 use the impact on diarrhea of weaned piglets rate before and after compound acidulant
Table 2 uses the impact on diarrhea of weaned piglets rate before and after compound acidulant
Note: colleague's data shoulder is designated as different lowercase alphabet and shows significant difference (P<0.05), has identical lowercase alphabet differential different not significantly (P>0.05), lower same.
Each processed group diarrhea rate sees the above table 2.As can be seen from Table 2, use before acidulant, each group diarrhea rate is all comparatively serious, close to or more than 20%, drinking-water is fed after acidulant, and two test group are all conducive to the digestive property improving piglet, reduce the diarrhea rate of piglet.F1 group reduction diarrhea rate reaches 23.9%, F2 group reduction diarrhea rate and reaches 8.9%, and matched group is not owing to adding any pharmaceutical preparation, and piglet diarrhea rate rises to some extent, it can be said that bright compound acidulant has good effect to alleviation diarrhea of weaned piglets.
2.2 compound acidulants are on the impact of Production Performance of Weaning Pigs
Table 3 compound acidulant is on the impact of Production Performance of Weaning Pigs
Table 3 shows, average opisthosoma weight, average daily gain that compound acidulant F1, F2 can significantly improve ablactational baby pig is added in drinking-water, but not significantly (P>0.05), F1 group significantly reduces feedstuff-meat ratio (P < 0.05) to difference compared with matched group.It can be said that bright compound acidulant can significantly improve the fertility performance of ablactational baby pig.
2.3 compound acidulants are on the impact of ablactational baby pig faecal microbiota
Table 4 compound acidulant is on the impact of ablactational baby pig faecal microbiota
There are some researches show, escherichia coli are the critical bacterial populations causing piglet diarrhea, and escherichia coli quantity declines, and is conducive to the microflora balance in piglet gastrointestinal tract, effectively prevents the generation of piglet diarrhea.As can be seen from Table 4, compound acidulant F1, F2 significantly can reduce colibacillary quantity (P < 0.05), thus effectively prevent the generation of piglet diarrhea.
2.4 compound acidulants are on impacts such as the crude protein of ablactational baby pig feces
Table 5 compound acidulant is on impacts such as the crude protein of ablactational baby pig feces
As can be seen from Table 5, F1 group energy, crude protein significantly lower than matched group (P < 0.05), illustrate that F1 improves protein utilization rate and S. E. A., facilitate the growth of pig; F2 group crude protein is significantly lower than matched group (P < 0.05), and all the other each component differences are not significantly (P>0.05).F1, F2 group all facilitates the growth of pig, reduces the energy in feces and crude protein level.
3 conclusions
Can draw from above-mentioned result of the test, compound acidulant can promotion piglet growth in various degree, reduce the escherichia coli quantity in intestinal, the generation of effective preventing post-weaning diarrhea, and test group I (F1) is better than test group II (F2).
Embodiment 2: a kind of preparation reducing the compound formulation of diarrhea of weaned piglets rate
This compound formulation be by oligosaccharide acidulant liquid and probiotics fermention liquid by volume 1:4 mix;
Wherein, oligosaccharide acidulant liquid is the compound acidulant formula 1 that optimized by embodiment 1 and oligosaccharide is composite forms, contain in the oligosaccharide acidulant liquid of every 1mL: oxalic acid 3000 μ g, tartaric acid 50 μ g, malic acid 60 μ g, lactic acid 5000 μ g, citric acid 3000 μ g, glucose 0.1g, oligomeric isomaltose 0.05g, oligofructose 0.05g, oligo-chitosan 0.1g;
Glucose, oligomeric isomaltose, oligofructose and oligo-chitosan are commercially available prod.
Probiotics fermention liquid be by Lactobacillus plantarum, Pediococcus pentosaceus mixed culture fermentation liquid and Clostridium butyricum fermentation liquid by volume 2:1 mix.Lactobacillus plantarum, Pediococcus pentosaceus mixed culture fermentation liquid is by Lactobacillus plantarum LP (Lactobacillus plantarum), Pediococcus pentosaceus PP (Pediococcus pentosaceus PP) co-fermentation obtains, this technology is documented in a patent of invention " a kind of lactobacillus semisolid fermentation product and technique improving growth of animal performance " (CN 102599348 B) of applicant, fermentation process is with " concrete fermentation technology " step (1)-(4) in embodiment 3 in this patent, wherein Pediococcus pentosaceus PP (Pediococcuspentosaceus PP) is preserved in China typical culture collection center, its deposit number is CCTCC NO:M 2012029, Lactobacillus plantarum LP (Lactobacillus plantarum) is documented in another patent of invention of applicant " strain can be used for lactobacillus and the application thereof of biological preservation " (CN 101914475 B), be preserved in China typical culture collection center, its deposit number is CCTCC M 2010150.
Clostridium butyricum fermentation liquid is fermented by Clostridium butyricum (Clostridium butyrium) Cb-2 to obtain, this Clostridium butyricum is documented in another patent of invention " strain has the Clostridium butyricum for the treatment of diarrhoea effect and the application as substitute of sodium butyrate thereof " (CN 102559550 B) of applicant, be preserved in China typical culture collection center, its deposit number is: CCTCC M2011384; Fermentation process is with step (i)-(v) of " 4.6 prepare Clostridium butyricum mycopowder " part in embodiment 1 in this patent.
Concrete preparation process is:
Oxalic acid, tartaric acid, malic acid, lactic acid, citric acid, glucose, oligomeric isomaltose, oligofructose and oligo-chitosan are mixed, use water as solvent, heat and stir, make it dissolve completely, cool, adjustment pH is 3.0-5.0, obtained oligosaccharide acidulant liquid, contain in the oligosaccharide acidulant liquid of every 1mL: oxalic acid 3000 μ g, tartaric acid 50 μ g, malic acid 60 μ g, lactic acid 5000 μ g, citric acid 3000 μ g, glucose 0.1g, oligomeric isomaltose 0.05g, oligofructose 0.05g, oligo-chitosan 0.1g;
By the 2:1 mixing by volume of Lactobacillus plantarum LP, Pediococcus pentosaceus PP mixed culture fermentation liquid and Clostridium butyricum Cb-2 fermentation liquid, obtained probiotics fermention liquid, wherein Lactobacillus plantarum LP viable count is 1 × 10 8cfu/mL, Pediococcus pentosaceus PP viable count is 1 × 10 8cfu/mL, Clostridium butyricum Cb-2 viable count is 1 × 10 7cfu/mL;
By obtained oligosaccharide acidulant liquid and the 1:4 mixing by volume of probiotics fermention liquid, to obtain final product.
Embodiment 3: compound formulation control diarrhea of weaned piglets demonstration test
1 materials and methods
1.1 EXPERIMENTAL DESIGN
Test is carried out in Ba Fubafu plant of drink township of Ningyang County of Shandong Province township, selects the ablactational baby pig 90 that the same kind of 30-33 age in days, body weight are close, is divided into matched group, test group, Related product group, each group 3 repetitions, each repetition 10 pigs.Matched group every day, drinking-water was fed clear water, test group drinking-water feed embodiment 2 prepare compound formulation (water dilutes 10 times), Related product group is fed general raw edge (water dilutes 10 times), and this product is purchased from Henan Pu Nier Biology Pharmacy Co., Ltd.
1.2 test daily rations, management, evaluation, data analysis are see embodiment 1.
2 results and analysis
2.1 compound formulations are on the impact of diarrhea of weaned piglets rate
Table 6 compound formulation is on the impact of diarrhea of weaned piglets rate
Diarrhea rate is the important indicator evaluating diarrhea of weaned piglets.There is not death in this test whole process, test group is added compound formulation of the present invention by drinking-water and prevented and treated diarrhea of weaned piglets.As can be seen from Table 6, compared with matched group, test group significantly can reduce piglet diarrhea rate 27% (P<0.05), and Related product significantly reduces piglet diarrhea rate 11.34% (P<0.05).Compared with Related product group, test group significantly reduces piglet diarrhea rate 15.66% (P<0.05), illustrates that test group effect is more excellent in reduction diarrhea of weaned piglets rate.
2.2 compound formulations are on the impact of Production Performance of Weaning Pigs
Table 7 compound formulation is on the impact of Production Performance of Weaning Pigs
As can be seen from Table 7, compared with matched group, test group can significantly improve the average daily gain 36.4% (P<0.05) of ablactational baby pig, reduces feedstuff-meat ratio 13.7% (P<0.05); Related product group difference is not all significantly (P > 0.05); Compared with Related product group, test group significantly improves average daily gain 25% (P<0.05), remarkable reduction feedstuff-meat ratio 12% (P<0.05), illustrates that test group effect in raising Production Performance of Weaning Pigs is more excellent.
2.3 compound formulations are on the impact of ablactational baby pig faecal microbiota
Table 8 compound formulation is on the impact of ablactational baby pig faecal microbiota
As can be seen from Table 8, compared with matched group, in test group feces, lactic acid bacterium number significantly increases (P<0.05), and escherichia coli quantity significantly reduces (P<0.05); Related product group is not on lactobacillus, the impact of escherichia coli quantity in piglet feces significantly (P > 0.05); Compared with Related product group, test group is significantly promoting lactobacillus intestinal colonisation, is suppressing escherichia coli proliferation effect more excellent (P<0.05).
2.4 compound formulations are on the impact of ablactational baby pig fecal energy, crude protein, crude fat
Table 9 compound formulation is on the impact of ablactational baby pig fecal energy, crude protein, crude fat
As can be seen from Table 9, compared with matched group, test group ablactational baby pig fecal energy, crude fat all significantly reduce (P<0.05), and crude protein difference is not significantly (P>0.05); Related product group is not on the impact of ablactational baby pig fecal energy, crude protein, crude fat all significantly (P>0.05); Compared with Related product group, test group piglet fecal energy, crude fat significantly reduce (P<0.05), improve the digestive utilization ratio of feedstuff.
2.5 compound formulations are on the impact of ablactational baby pig feces sIgA content
Table 10 compound formulation is on the impact of ablactational baby pig feces sIgA content
As can be seen from Table 10, compared with matched group, test group feces sIgA content significantly raises 20.8% (P<0.05), illustrates that test group can significantly improve piglet feces sIgA content, enhances the mucosa-immune power of ablactational baby pig; Related product group is not on the impact of sIgA content significantly (P>0.05); Test group sIgA content significantly improves about 18.7% (P<0.05) than Related product group, in enhancing mucosa-immune, and test group better effects if.
3 discuss
3.1 these compound formulations are on the impact of diarrhea of weaned piglets rate
Piglet diarrhea affects by many factors.Diarrhoea is lacked of proper care due to enteropahtogenic microganism and is caused, result of study of the present invention finds, drinking-water adds the compound formulation be mixed by oligosaccharide, acidulant and probiotic bacteria can significantly reduce diarrhea of weaned piglets rate 27%, and Related product is only 11.34%, use the diarrhea rate after compound formulation to reduce 15.66% than Related product group, visible compound formulation of the present invention effect in reduction diarrhea of weaned piglets rate is better.
3.2 these compound formulations are on the impact of Production Performance of Weaning Pigs
This result of study finds, compared with matched group, this compound formulation significantly improves average daily gain 36.4%, reduces feedstuff-meat ratio 13.7%, compared with Related product group, compound formulation significantly improves average daily gain 25%, and reduce feedstuff-meat ratio 12%, this compound formulation improves the fertility performance of ablactational baby pig as seen, may be because compound formulation has sour fragrance, have stimulated the appetite of ablactational baby pig, improve piglet daily gain, reduce the cause of feedstuff-meat ratio.
3.3 these compound formulations are on the impact of ablactational baby pig faecal microbiota
In the formula of compound formulation of the present invention: oligosaccharide, probiotic bacteria, acidulant all can improve microbiota in intestinal, suppress the growth of harmful bacteria, promote the propagation of probiotics.First, acidulant can reduce gastrointestinal pH value, suppresses the breeding regeneration of pathogen; Secondly, the interpolation of probiotic bacteria can produce positive effect to the field planting of probiotics in intestinal; Finally, oligosaccharide, except can supplementing certain energy, can also show similar antibiotic feature, suppress the growth of multiple pathogen.Three effectively reduces the quantity of pathogenic entero becteria, thus ensure that the health of intestinal.Test also proves, test group (drinking compound formulation group) improves microbiota in intestinal, suppresses the growth of harmful bacteria, promotes the propagation of probiotics.
3.4 these compound formulations are on the impact of ablactational baby pig fecal energy, crude protein, crude fat
This compound formulation can be improved the utilization rate of piglet to feedstuff and be realized by many factors.First, reduce acidity and can improve various enzymatic activity in the intestines and stomach; Secondly, the interpolation of probiotic bacteria also plays certain facilitation to the breeding of bacillus cereus; Again, the utilization that product is sour, bacteriostasis efficacy is also conducive to feedstuff of Clostridium butyricum; In this test data display test group feces, energy, fat significantly decline, and illustrate that the interpolation of this compound formulation can improve efficiency of feed utilization.
3.5 these compound formulations are on the impact of ablactational baby pig feces sIgA content
Secretory IgA and sIgA are the main antibody of body mucosa local anti-infective immunity.Therefore also known as mucosa local antibody, the high mental retardation of its content represents the level of mucosa-immune.A large number of experiments show that, after animal probiotic oral, can adjust intestinal microbial population, make gastrointestinal micro-flora be in best poised state, associated lymphoid tissue in activation intestinal mucosa, makes sIgA antibody-secreting strengthen.This result of the test finds, compared with matched group, Related product group, test group compound formulation significantly improves sIgA content, illustrates that this compound formulation can strengthen mucosa-immune power, improves body's immunity and premunition.
4 conclusions
Compound formulation of the present invention, compared with matched group, Related product group, significantly can reduce the diarrhea rate of ablactational baby pig; Significantly improve piglet average daily gain, significantly reduce feedstuff-meat ratio, improve the fertility performance of piglet; Lactic acid bacterium number in remarkable increase feces, reduces escherichia coli quantity, balance intestinal flora; Remarkable reduction ablactational baby pig fecal energy and crude fat content, improve efficiency of feed utilization; Significantly improve feces sIgA content, improve mucosa-immune power; Eventually through above-mentioned comprehensive function, this compound formulation reduces the diarrhea rate of ablactational baby pig.

Claims (9)

1. reducing a compound formulation for diarrhea of weaned piglets rate, it is characterized in that, is by oligosaccharide acidulant liquid and probiotics fermention liquid 1:(3-5 by volume) mix;
Contain in the described oligosaccharide acidulant liquid of every 1mL:
Oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid 50-100 μ g, lactic acid 4000-5500 μ g, citric acid 2500-4000 μ g, glucose 0.05-0.2g, oligomeric isomaltose 0.05-0.1g, oligofructose 0.02-0.06g, oligo-chitosan 0.08-0.15g;
Described probiotics fermention liquid is by Lactobacillus plantarum, Pediococcus pentosaceus mixed culture fermentation liquid and Clostridium butyricum fermentation liquid by volume (2-4): 1 mixes.
2. a kind of compound formulation reducing diarrhea of weaned piglets rate as claimed in claim 1, is characterized in that, in described probiotics fermention liquid, the viable count of Lactobacillus plantarum is greater than or equal to 1 × 10 8cfu/mL, the viable count of Pediococcus pentosaceus is greater than or equal to 1 × 10 8cfu/mL, the viable count of Clostridium butyricum is greater than or equal to 1 × 10 7cfu/mL.
3. a kind of compound formulation reducing diarrhea of weaned piglets rate as claimed in claim 1, is characterized in that, described probiotics fermention liquid be by Lactobacillus plantarum, Pediococcus pentosaceus mixed culture fermentation liquid and Clostridium butyricum fermentation liquid by volume 2:1 mix.
4. a kind of compound formulation reducing diarrhea of weaned piglets rate as claimed in claim 1, it is characterized in that, described Lactobacillus plantarum, Pediococcus pentosaceus mixed culture fermentation liquid are obtained by the Pediococcus pentosaceus PP co-fermentation of deposit number to be the Lactobacillus plantarum LP of CCTCC M 2010150 and deposit number be CCTCC NO:M 2012029.
5. a kind of compound formulation reducing diarrhea of weaned piglets rate as claimed in claim 1, is characterized in that, described Clostridium butyricum fermentation liquid is that the Clostridium butyricum Cb-2 fermentation being CCTCC M 2011384 by deposit number obtains.
6. a kind of compound formulation reducing diarrhea of weaned piglets rate as claimed in claim 1, it is characterized in that, contain in the described oligosaccharide acidulant liquid of every 1mL: oxalic acid 3000 μ g, tartaric acid 50 μ g, malic acid 60 μ g, lactic acid 5000 μ g, citric acid 3000 μ g, glucose 0.1g, oligomeric isomaltose 0.05g, oligofructose 0.05g, oligo-chitosan 0.1g.
7. a kind of compound formulation reducing diarrhea of weaned piglets rate as claimed in claim 1, is characterized in that, described oligo-chitosan is polymerized by 2-10 glycosidic bond.
8. reduce a preparation method for the compound formulation of diarrhea of weaned piglets rate, it is characterized in that, step is as follows:
(1) by oxalic acid, tartaric acid, malic acid, lactic acid, citric acid, glucose, oligomeric isomaltose, oligofructose and oligo-chitosan mixing, use water as solvent, heat and stir, it is made to dissolve completely, cooling, adjustment pH is 3.0-5.0, obtained oligosaccharide acidulant liquid, contain in the oligosaccharide acidulant liquid of every 1mL: oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid 50-100 μ g, lactic acid 4000-5500 μ g, citric acid 2500-4000 μ g, glucose 0.05-0.2g, oligomeric isomaltose 0.05-0.1g, oligofructose 0.02-0.06g, oligo-chitosan 0.08-0.15g,
(2) by Lactobacillus plantarum LP, Pediococcus pentosaceus PP mixed culture fermentation liquid and Clostridium butyricum Cb-2 fermentation liquid by volume (2-4): 1 mixing, obtained probiotics fermention liquid, wherein the viable count of Lactobacillus plantarum LP, Pediococcus pentosaceus PP and Clostridium butyricum Cb-2 is greater than or equal to 1 × 10 respectively 8cfu/mL, 1 × 10 8cfu/mL and 1 × 10 7cfu/mL;
(3) the probiotics fermention liquid 1:(3-5 by volume that oligosaccharide acidulant liquid step (1) obtained and step (2) obtain) mixing, to obtain final product.
9. a kind of preparation method reducing the compound formulation of diarrhea of weaned piglets rate as claimed in claim 8, is characterized in that, in step (3), and the 1:4 mixing by volume of oligosaccharide acidulant liquid and probiotics fermention liquid.
CN201510102291.XA 2015-03-06 2015-03-06 A kind of compound formulation and preparation method thereof reducing diarrhea of weaned piglets rate Active CN104758318B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510102291.XA CN104758318B (en) 2015-03-06 2015-03-06 A kind of compound formulation and preparation method thereof reducing diarrhea of weaned piglets rate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510102291.XA CN104758318B (en) 2015-03-06 2015-03-06 A kind of compound formulation and preparation method thereof reducing diarrhea of weaned piglets rate

Publications (2)

Publication Number Publication Date
CN104758318A true CN104758318A (en) 2015-07-08
CN104758318B CN104758318B (en) 2018-11-30

Family

ID=53640647

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510102291.XA Active CN104758318B (en) 2015-03-06 2015-03-06 A kind of compound formulation and preparation method thereof reducing diarrhea of weaned piglets rate

Country Status (1)

Country Link
CN (1) CN104758318B (en)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105248877A (en) * 2015-09-11 2016-01-20 山东宝来利来生物工程股份有限公司 Micro-ecological compound preparation with immunological enhancement function
CN105360665A (en) * 2015-11-16 2016-03-02 广州暨南生物医药研究开发基地有限公司 Anti-diarrhea Chinese herbal medicine feed additive for weaned piglets and preparation method thereof
CN106343189A (en) * 2016-08-26 2017-01-25 湖南百宜饲料科技有限公司 Fermented semi-fluid feed for newborn piglets and production and preparation method of fermented semi-fluid feed
CN106509468A (en) * 2016-11-10 2017-03-22 四川理工学院 Composite type synbiotic microecological preparation for chickens and preparation method of composite type synbiotic microecological preparation
CN106509455A (en) * 2016-11-10 2017-03-22 四川理工学院 Composite type synbiotic microecological preparation for rabbits and preparation method of composite type synbiotic microecological preparation
CN106566791A (en) * 2016-11-10 2017-04-19 四川理工学院 Porcine compound synbiotics micro-ecological preparation and preparation method thereof
CN106689753A (en) * 2017-01-04 2017-05-24 陈雨 Weaned piglet feed compound preparation, feed containing the compound preparation and application of the feed
CN106720938A (en) * 2016-11-10 2017-05-31 四川理工学院 A kind of compound symphysis unit probiotics and preparation method thereof
CN108391758A (en) * 2018-04-25 2018-08-14 广西康佳龙农牧集团有限公司 Milking sow compound Synbiotics
CN109136126A (en) * 2018-08-21 2019-01-04 云南农业大学 One plant of Pediococcus pentosaceus for preventing post-weaning diarrhea
CN109770046A (en) * 2019-03-08 2019-05-21 刘坚 A kind of sucking pig wean pannage and preparation method thereof
CN111657402A (en) * 2020-06-30 2020-09-15 武汉合缘绿色生物股份有限公司 Feed additive for regulating intestinal function of pigs and preparation method thereof
CN112586621A (en) * 2020-12-15 2021-04-02 南京致润生物科技有限公司 Functional fermented yoghourt special for piglets and preparation method thereof
CN112618579A (en) * 2020-12-24 2021-04-09 南京农业大学 Compound probiotic preparation for preventing and treating piglet diarrhea as well as preparation method and application thereof

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1860911A (en) * 2006-06-09 2006-11-15 大连轻工业学院 Composite microorganism type forage additives and its prodn. method
CN102488117A (en) * 2011-12-22 2012-06-13 南京农业大学 Combination used for preventing and treating newborn pig diarrhea and preparation method and application thereof
CN102559550A (en) * 2011-12-30 2012-07-11 山东宝来利来生物工程股份有限公司 Clostridium butyricum having diarrhea treating effect and application of clostridium butyricum as substitute of sodium butyrate
CN102599348A (en) * 2012-03-26 2012-07-25 山东宝来利来生物工程股份有限公司 Lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals and preparation process thereof
CN102823771A (en) * 2012-09-07 2012-12-19 北京大北农科技集团股份有限公司 Biological active acidifying agent for piglets, premix compound and compound
CN102870987A (en) * 2012-09-19 2013-01-16 广州立达尔生物科技股份有限公司 Green feed additive used for improving development of intestinal canal of infant animal
CN102960560A (en) * 2012-11-23 2013-03-13 重庆优宝生物技术有限公司 Composite acidifier for reducing post-weaning diarrhea rate
WO2013101019A1 (en) * 2011-12-29 2013-07-04 Hill's Pet Nutrition, Inc. Compositions and methods for modifying gastrointestinal flora
CN103876145A (en) * 2014-04-01 2014-06-25 吉林大学 Probiotics micro-ecological tablet and preparation method thereof
CN104171363A (en) * 2014-07-02 2014-12-03 中泰和(北京)科技发展有限公司 Feed additive for reducing diarrhea rate of piglets

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1860911A (en) * 2006-06-09 2006-11-15 大连轻工业学院 Composite microorganism type forage additives and its prodn. method
CN102488117A (en) * 2011-12-22 2012-06-13 南京农业大学 Combination used for preventing and treating newborn pig diarrhea and preparation method and application thereof
WO2013101019A1 (en) * 2011-12-29 2013-07-04 Hill's Pet Nutrition, Inc. Compositions and methods for modifying gastrointestinal flora
CN102559550A (en) * 2011-12-30 2012-07-11 山东宝来利来生物工程股份有限公司 Clostridium butyricum having diarrhea treating effect and application of clostridium butyricum as substitute of sodium butyrate
CN102599348A (en) * 2012-03-26 2012-07-25 山东宝来利来生物工程股份有限公司 Lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals and preparation process thereof
CN102823771A (en) * 2012-09-07 2012-12-19 北京大北农科技集团股份有限公司 Biological active acidifying agent for piglets, premix compound and compound
CN102870987A (en) * 2012-09-19 2013-01-16 广州立达尔生物科技股份有限公司 Green feed additive used for improving development of intestinal canal of infant animal
CN102960560A (en) * 2012-11-23 2013-03-13 重庆优宝生物技术有限公司 Composite acidifier for reducing post-weaning diarrhea rate
CN103876145A (en) * 2014-04-01 2014-06-25 吉林大学 Probiotics micro-ecological tablet and preparation method thereof
CN104171363A (en) * 2014-07-02 2014-12-03 中泰和(北京)科技发展有限公司 Feed additive for reducing diarrhea rate of piglets

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
周盟 等: "益生菌对断奶仔猪生长性能、免疫器官指数及胃肠道pH的影响", 《动物营养学报》 *
徐海燕 等: "微生态制剂在断奶仔猪生产上的应用研究", 《青岛农业大学学报(自然科学版)》 *
杨长庚等: "动物微生态制剂的研究进展及存在问题", 《畜牧生产》 *
王杰 等: "复合益生菌和纤维寡糖对断奶仔猪生长性能、粪便微生物及血清指标的影响", 《动物营养学报》 *
王杰 等: "复合酸化剂对断奶仔猪生产性能和饲料表观消化率的影响", 《黑龙江畜牧兽医》 *
谢全喜 等: "复合微生态制剂对哺乳仔猪生产性能和粪便菌群的影响", 《饲料博览》 *
陈代文 等: "酸化剂、益生素和寡糖对断奶仔猪粪中微生物菌群和免疫功能的影响及其互作效应研究", 《动物营养学报》 *
魏建英 等: "《发酵床环保节能养猪技术》", 31 October 2013 *

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105248877B (en) * 2015-09-11 2018-10-02 山东宝来利来生物工程股份有限公司 A kind of micro-ecological compound preparation with immune enhancing function
CN105248877A (en) * 2015-09-11 2016-01-20 山东宝来利来生物工程股份有限公司 Micro-ecological compound preparation with immunological enhancement function
CN105360665A (en) * 2015-11-16 2016-03-02 广州暨南生物医药研究开发基地有限公司 Anti-diarrhea Chinese herbal medicine feed additive for weaned piglets and preparation method thereof
CN106343189A (en) * 2016-08-26 2017-01-25 湖南百宜饲料科技有限公司 Fermented semi-fluid feed for newborn piglets and production and preparation method of fermented semi-fluid feed
CN106343189B (en) * 2016-08-26 2019-11-08 湖南百宜饲料科技有限公司 A kind of the fermentation semi-fluid body feedstuff and its preparation method of newborn piglet
CN106509455A (en) * 2016-11-10 2017-03-22 四川理工学院 Composite type synbiotic microecological preparation for rabbits and preparation method of composite type synbiotic microecological preparation
CN106720938A (en) * 2016-11-10 2017-05-31 四川理工学院 A kind of compound symphysis unit probiotics and preparation method thereof
CN106566791A (en) * 2016-11-10 2017-04-19 四川理工学院 Porcine compound synbiotics micro-ecological preparation and preparation method thereof
CN106509468A (en) * 2016-11-10 2017-03-22 四川理工学院 Composite type synbiotic microecological preparation for chickens and preparation method of composite type synbiotic microecological preparation
CN106689753A (en) * 2017-01-04 2017-05-24 陈雨 Weaned piglet feed compound preparation, feed containing the compound preparation and application of the feed
CN108391758A (en) * 2018-04-25 2018-08-14 广西康佳龙农牧集团有限公司 Milking sow compound Synbiotics
CN109136126A (en) * 2018-08-21 2019-01-04 云南农业大学 One plant of Pediococcus pentosaceus for preventing post-weaning diarrhea
CN109136126B (en) * 2018-08-21 2021-04-13 云南农业大学 Pediococcus pentosaceus for preventing diarrhea of weaned piglets
CN109770046A (en) * 2019-03-08 2019-05-21 刘坚 A kind of sucking pig wean pannage and preparation method thereof
CN111657402A (en) * 2020-06-30 2020-09-15 武汉合缘绿色生物股份有限公司 Feed additive for regulating intestinal function of pigs and preparation method thereof
CN112586621A (en) * 2020-12-15 2021-04-02 南京致润生物科技有限公司 Functional fermented yoghourt special for piglets and preparation method thereof
CN112618579A (en) * 2020-12-24 2021-04-09 南京农业大学 Compound probiotic preparation for preventing and treating piglet diarrhea as well as preparation method and application thereof
CN112618579B (en) * 2020-12-24 2022-02-15 南京农业大学 Compound probiotic preparation for preventing and treating piglet diarrhea as well as preparation method and application thereof

Also Published As

Publication number Publication date
CN104758318B (en) 2018-11-30

Similar Documents

Publication Publication Date Title
CN104758318A (en) A composite preparation for reducing a diarrhea rate of weaned piglets and a preparing method thereof
CN103798553B (en) Environment-friendly pig feed additive and preparation method thereof
CN102406051B (en) Production method, using method and production equipment of high-activity nonreactive feed
CN106071250A (en) A kind of fattening pannage and preparation method thereof
Zduńczyk et al. Determinants and effects of postileal fermentation in broilers and turkeys part 1: gut microbiota composition and its modulation by feed additives
CN104543411B (en) Yeast complex additive with high-efficiency food attraction and immunological enhancement functions
CN103907749A (en) Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof
CN103392922A (en) Compound feed additive capable of improving health level of animal
CN102697888A (en) Traditional Chinese medicine fermented preparation for preventing and treating piglet diarrhea and preparation method thereof
CN106689753A (en) Weaned piglet feed compound preparation, feed containing the compound preparation and application of the feed
CN106509440A (en) Health-care feed additive for multiparous sows
CN103330059A (en) Health care composition for livestock as well as preparation method and application thereof
CN103550543B (en) A kind of Chinese medicine and its preparation method treating grice diarrhoea
CN104705528A (en) Multivitamin fermented Chinese herbal medicine preparation special for breeding sows as well as preparation method and application thereof
CN106922997A (en) A kind of pig feed additive of efficient gastrointestinal mucosa repair function
CN104206789A (en) Feed additive for preventing cow mastitis and brucellosis and preparation method thereof
CN100482104C (en) Process for preparing lactic acid bacteria oral liquor
CN101999556B (en) Special animal nutrient for beef cattle and preparation method thereof
CN106417989A (en) Probiotic composition for aquatic animal feed, preparation method and application thereof
CN101999555A (en) Animal nutrient special for sheep and method for preparing the same
CN106135659B (en) A kind of high-efficiency and environment-friendly feed and preparation method thereof improving meat quality
CN106538825A (en) Poultry nonreactive feedstuff
CN105124357A (en) Nutrition bag capable of regulating intestinal microecological environment and relieving internal heat of baby
CN109497308A (en) Piglet anti diar rhea additive for microbe feedstuff and preparation method thereof
CN102715129B (en) The raising method of a kind of herbal medicine chicken and feeds utilized

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
EXSB Decision made by sipo to initiate substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant