CN104758318B - A kind of compound formulation and preparation method thereof reducing diarrhea of weaned piglets rate - Google Patents
A kind of compound formulation and preparation method thereof reducing diarrhea of weaned piglets rate Download PDFInfo
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Abstract
It is by oligosaccharide acidulant liquid and probiotics fermention liquid by volume 1 the invention discloses a kind of compound formulation for reducing diarrhea of weaned piglets rate:(3-5) is mixed；Contain in the oligosaccharide acidulant liquid of every 1mL：Oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid 50-100 μ g, lactic acid 4000-5500 μ g, citric acid 2500-4000 μ g, glucose 0.05-0.2g, oligoisomaltose 0.05-0.1g, oligofructose 0.02-0.06g, chitosan oligomer 0.08-0.15g；The probiotics fermention liquid be by lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid and clostridium butyricum fermentation liquid by volume (2-4):1 mixes.The invention also discloses the preparation methods of the reduction diarrhea of weaned piglets rate compound formulation.Compound formulation of the invention can reduce diarrhea of weaned piglets probability, reduce the death rate of piglet, improve the production performance and immune function of piglet.
Description
Technical field
The present invention relates to a kind of compound formulations and preparation method thereof for reducing diarrhea of weaned piglets rate.
Background technique
In recent years, pig breeding industry development in China's is very rapid, and the management of cultivation and large-scale degree are all improved.With
Early weaning piglet skill has gradually been popularized in the countries and regions of the raising of pig production technical level, some pig breeding industry prosperities
Art, but cross early-weaning can make piglet nutrition, environment and it is psychological generate stress, lead to feed intake reduction, growth retardation, weight
Decline, diarrhea, in addition it is dead.It is reported that 30kg piglet below, annual diarrhea disease percentage 46.5%, the death rate 10.3%.Cause
How this, prevent and treat one of the urgent task that diarrhea of weaned piglets has become current pig breeding industry.
Up to the present, domestic to mostly use antibiotic etc direct injection in the prevention and treatment of diarrhea of weaned piglets disease or add
It is added in feed, with a large amount of appearance of antibody-resistant bacterium, it is ineffective to be frequently found existing chemicals control diarrhea, and this
The a large amount of of a little drugs are enriched with the health for seriously threatening the mankind using livestock products drug residue is be easy to cause in human body；Epidemic disease
Seedling is immune, though the antibody content in sow milk can be improved, the antibody duration is short, is not enough to protect the entire growth phase of piglet
Diarrhea does not occur, and e. coli serotype is complicated, constantly variation, it is little immune piglet is produced effects；Chinese herbal medicine drug effect is slow
Slowly, dosage is big, and palatability is poor, limits its application in weanling pig daily ration；The microelements such as high-copper, high zinc also have
It is applied, but easily residual, pollution are big, easily lead to heavy metal poisoning；Enzyme preparation is also used, but position is excessively single, nothing
Diarrhea caused by method is solved due to intestinal bacilli illness.Therefore, find it is a kind of diarrhea of weaned piglets rate can be effectively reduced, simultaneously
The product for not endangering human health is imperative.
Summary of the invention：
In view of the deficiency of the prior art, the object of the present invention is to provide a kind of reduction diarrhea of weaned piglets rates
Compound formulation and preparation method thereof, the compound formulation can reduce diarrhea of weaned piglets rate, reduce the death rate of piglet, improve piglet
Production performance and immune function.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of compound formulation reducing diarrhea of weaned piglets rate, is by oligosaccharide acidulant liquid and probiotics fermention liquid by body
Product ratio 1:(3-5) is mixed；
Contain in the oligosaccharide acidulant liquid of every 1mL：
Oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid 50-100 μ g, lactic acid 4000-5500 μ g, citric acid
2500-4000 μ g, glucose 0.05-0.2g, oligoisomaltose 0.05-0.1g, oligofructose 0.02-0.06g, oligopolymerization chitosan
Sugared 0.08-0.15g；
Preferably, contain in the oligosaccharide acidulant liquid of every 1mL：3000 μ g of oxalic acid, 50 μ g of tartaric acid, malic acid 60
μ g, 5000 μ g of lactic acid, citric acid 3000 μ g, glucose 0.1g, oligoisomaltose 0.05g, oligofructose 0.05g, oligomeric shell
Glycan 0.1g.
Preferably, the chitosan oligomer is polymerized by 2-10 glycosidic bond.
The probiotics fermention liquid be by lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid and clostridium butyricum fermentation liquid by
Volume ratio (2-4):1 mixes, and in the probiotics fermention liquid, the viable count of lactobacillus plantarum is more than or equal to 1 ×
108The viable count of cfu/mL, Pediococcus pentosaceus are more than or equal to 1 × 108The viable count of cfu/mL, clostridium butyricum be greater than or
Equal to 1 × 107cfu/mL。
Preferably, the probiotics fermention liquid is by lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid and clostridium butyricum
Fermentation liquid by volume 2:1 mixes.
The lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid are by lactobacillus plantarum LP (Lactobacillus
Plantarum), Pediococcus pentosaceus PP (Pediococcus pentosaceus PP) co-fermentation obtains, which is documented in
An a kind of patent of invention " lactic acid bacteria semisolid fermentation product and technique for improving growth of animal performance " (CN of applicant
In 102599348B), fermentation process is the same as " specific zymotechnique " step (1)-(4) in embodiment 3 in the patent；Wherein pentose piece
Coccus PP (Pediococcus pentosaceus PP) is preserved in China typical culture collection center, and deposit number is
CCTCC NO：M 2012029；Lactobacillus plantarum LP (Lactobacillus plantarum) is documented in another hair of applicant
In bright patent " one plant of lactic acid bacteria that can be used for biological preservation and its application " (CN101914475 B), it is preserved in Chinese allusion quotation
Type culture collection, deposit number are CCTCC NO：M 2010150.
The clostridium butyricum fermentation liquid is to ferment to obtain by clostridium butyricum (Clostridium butyricum) Cb-2, should
Clostridium butyricum is documented in another patent of invention of applicant, and " one plant has the clostridium butyricum for the treatment of diarrhea effect and its as fourth
In the application of sour sodium substitute " (102559550 B of CN), it is preserved in China typical culture collection center, deposit number
For：CCTCC NO：M 2011384；Fermentation process is the same as " 4.6 prepare clostridium butyricum bacterium powder " part in embodiment 1 in the patent
Step (i)-(v) ".
In the prescription of the compound formulation, oligosaccharide is small molecule carbohydrate, since it cannot be disappeared by alimentary canal endogenous enzymes
Change, cannot also be utilized by harmful bacteria, thus beneficial bacterium growth, breeding can be promoted, so that balance intestinal flora, inhibits the hair of diarrhea
It is raw.Probiotics fermention liquid has regulation intestinal flora balance, inhibits the breeding of the harmful bacterias such as Escherichia coli, salmonella, prevention
The effect of diarrhea of weaned piglets.Acidulant is the monomer or mixture for referring to be added to organic acid and inorganic acid in feed,
It is acted on mainly by improving gastrointestinal micro-flora, is inhibited the growth of harmful bacteria, is promoted the proliferation of beneficial bacterium；It adjusts in stomach
PH value and the intestine evacuation velocity for slowing down stomach, to promote the digestion and absorption of nutriment；The palatability for improving feed, whets the appetite.
Three's compounding can reduce diarrhea of weaned piglets rate.
The preparation method of the reduction diarrhea of weaned piglets rate compound formulation, steps are as follows：
(1) by oxalic acid, tartaric acid, malic acid, lactic acid, citric acid, glucose, oligoisomaltose, oligofructose and low
Chitosan mixing, uses water as solvent, heats and stir, make it completely dissolved, cool down, and adjusting pH is 3.0-5.0, is made low
Glycan acidulant liquid contains in the oligosaccharide acidulant liquid of every 1mL：Oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid
50-100 μ g, lactic acid 4000-5500 μ g, citric acid 2500-4000 μ g, glucose 0.05-0.2g, oligoisomaltose 0.05-
0.1g, oligofructose 0.02-0.06g, chitosan oligomer 0.08-0.15g；
(2) by volume by lactobacillus plantarum LP, Pediococcus pentosaceus PP mixed fermentation liquid and clostridium butyricum Cb-2 fermentation liquid
(2-4):1 mixes, in the probiotics fermention liquid, the work of lactobacillus plantarum LP, Pediococcus pentosaceus PP and clostridium butyricum Cb-2
Bacterium number is respectively greater than or is equal to 1 × 108cfu/mL、1×108Cfu/mL and 1 × 107cfu/mL；
(3) by probiotics fermention liquid made from oligosaccharide acidulant liquid made from step (1) and step (2) by volume 1:
(3-5) mixing to get.
Preferably, in the step (3), oligosaccharide acidulant liquid and probiotics fermention liquid by volume 1:4 mixing.
Beneficial effects of the present invention：
(1) present invention is compounded using oligosaccharide, probiotics, acidulant for the first time, and wean can be effectively reduced by having developed
The product of grice diarrhoea rate；Wherein oligosaccharide, probiotics, acidulant three synergistic effect, on the one hand, oligosaccharide is small molecule object
Matter can be made full use of by probiotics, and without being consumed by harmful bacteria, final balance intestinal flora reduces the probability of happening of diarrhea；Separately
On the one hand, lactic acid bacteria produces acid, reduces the quantity of pathogenic bacteria, and clostridium butyricum Antidiarrheic effect is good, the two is compound to reduce abdomen
The generation rushed down；The third aspect, acidulant can reduce gut pH, promote lactic acid bacteria field planting, are conducive to producing enzyme, improve feed digestion
Utilization rate.
(2) present invention selects clostridium butyricum, lactobacillus plantarum and Pediococcus pentosaceus to be compounded, and wherein clostridium butyricum is metabolized
The organic acids such as butyric acid, acetic acid are generated, bacteriostasis rate is high；Lactobacillus plantarum, Pediococcus pentosaceus are enteron aisle dominant microflora, can be in enteron aisle
Establish dominant microflora；Oligomeric functional sugar simultaneously can promote beneficial bacterium growth, breeding, not utilized by harmful bacteria, chitosan oligomer is also
There is the effect of similar antibiotic, by adjusting intestine microenvironment, plays the anti-diarrhea effect of growth promotion.
(3) effect of compound formulation of the present invention is evaluated by excrement count plate and S-IgA assay
As a result fruit proves that compound formulation of the invention can significantly reduce the diarrhea rate of weanling pig；Piglet average daily gain is improved, it is average
Daily ingestion amount significantly reduces feedstuff-meat ratio, improves the production performance of piglet；Lactic acid bacterium number in excrement is dramatically increased, large intestine is reduced
Bacillus quantity, balance intestinal flora；Weanling pig fecal energy and crude fat content are significantly reduced, efficiency of feed utilization is improved；It is aobvious
It writes and improves excrement sIgA content, enhance mucosa-immune ability.
Detailed description of the invention
Fig. 1 a：It is illustrated for control group piglet excrement in embodiment 3, watery stools are more；
Fig. 1 b：It is illustrated for test group piglet excrement in embodiment 3, excrement forms, is harder；
Fig. 1 c：It is illustrated for Related product group excrement in embodiment 3, excrement is thin, soft stool.
Specific embodiment
The present invention is further illustrated in conjunction with the embodiments, it should which explanation, following the description is merely to explain this
Invention, is not defined its content.
Embodiment 1：The compound acidulant screening test for preventing and treating diarrhea of weaned piglets
1 materials and methods
1.1 experimental design
Test is carried out in drink township Ba Fubafu farm of Ningyang County of Shandong Province township, selects the same kind of 30-33 age in days, weight phase
Close weanling pig 90, is divided into control group (CK), test group I (F1) and test group II (F2), each group of 3 repetitions, each
Repeat 10 pigs.Control group is drunk water daily feeds clear water, and test group I feeds compound acidulant formula 1 (water dilutes 10 times) daily,
Test group II drinks water feeding compound acidulant formula 2 (10 times of water dilution) daily, continuously feeds 14d, changes into after feeding clear
Water is normally fed, and observes 7d later, examines pig diarrhea situation.
Compound acidulant formula 1：Every 1mL compound acidulant 3000 μ g containing oxalic acid, 50 μ g of tartaric acid, 60 μ g of malic acid, cream
Acid 5000 μ g, 3000 μ g of citric acid；
Compound acidulant formula 2：Every 1mL compound acidulant 300 μ g containing oxalic acid, 100 μ g of tartaric acid, 1000 μ g of lactic acid, second
Acid 1000 μ g, 500 μ g of citric acid.
1.2 test daily rations
Daily ration basic components are corn 58%, dregs of beans 24%, wheat bran 3.8%, fish meal (import) 5%, whey powder 5%, plant
Object oil 1%, mountain flour 0.7%, CaHCO31%, salt 0.2%, L lysine HCL 0.3%, premix 1%.Its nutritive water
It puts down as CP (crude protein) 9.8%, Ca 0.87%, P 0.05%, lysine 1.31% (being mass percent).
1.3 feeding management
Daily feeding 4 times, is freely eaten, and when control group fed supplies clear water, test group I, test group II compound acidulant
It drinks water and feeds after being diluted with water 10 times.Each pigsty of sweeping is given up 1 time sooner or later daily, every 7d cleaning and disinfection crib, apparatus 1 time, young
Thin observation feeding and excrement situation of change.Test period is 21d, respectively in test 0d, 21d morning 9:00 weighs on an empty stomach,
Calculate test group I, test group II and average daily gain, average daily gain, feedstuff-meat ratio of control group piglet etc..
1.4 diarrhea rates calculate
The daily diarrhea head number of piglet is recorded, is calculated：
Diarrhea rate=[circle day diarrhea piglet head number summation/(total head number × number of days of piglet)] × 100% grice diarrhoea journey
Degree evaluating basis is shown in Table 1.When occur mild diarrhea be same day excrement under normal conditions, be calculated as diarrhea head time 1 time.
1 Scours index evaluating basis of table
1.5 faecal microbiotas count and trophic level measurement
When off-test, test group, control group take appropriate excrement to take back experimental determination faecal microbiota and nutritive water respectively
Flat (energy, crude protein, crude fat etc.).Faecal microbiota is counted to be carried out using gradient dilution method, and the measurement of excrement trophic level uses
Energy measuring device, crude protein, determinator for crude fats are measured.
The analysis of 1.6 data carries out data analyses using SPSS software, is as a result indicated with X ± S.
2 results and analysis
2.1 use the influence before and after compound acidulant to diarrhea of weaned piglets rate
Table 2 uses the influence before and after compound acidulant to diarrhea of weaned piglets rate
Note：Colleague's data shoulder is designated as the significant (P of different lowercase letter indication differences<0.05) identical lowercase, indicates
Not significant (the P of difference>0.05), similarly hereinafter.
Each processing group diarrhea rate sees the above table 2.From table 2 it can be seen that each group diarrhea rate is all more serious before using acidulant,
Near or above 20%, after drinking-water feeds acidulant, two test groups are all conducive to improve the digestive property of piglet, reduce
The diarrhea rate of piglet.F1 group reduction diarrhea rate reaches 23.9%, F2 group reduction diarrhea rate and reaches 8.9%, and control group is not due to
Any pharmaceutical preparation is added, grice diarrhoea rate is risen, it is possible thereby to illustrate compound acidulant to alleviation diarrhea of weaned piglets
There is good effect.
Influence of 2.2 compound acidulants to Production Performance of Weaning Pigs
Influence of 3 compound acidulant of table to Production Performance of Weaning Pigs
Table 3 shows that compound acidulant F1, F2 are added in drinking-water can significantly improve the average opisthosoma of weanling pig again, averagely
Daily gain, average daily gain, but the not significant (P of difference compared with the control group>0.05), F1 group significantly reduces feedstuff-meat ratio (P <
0.05).It is possible thereby to illustrate that compound acidulant can significantly improve the production performance of weanling pig.
Influence of 2.3 compound acidulants to weanling pig faecal microbiota
Influence of 4 compound acidulant of table to weanling pig faecal microbiota
Some researches show that Escherichia coli are the critical bacterial populations for leading to grice diarrhoea, and the decline of Escherichia coli quantity is conducive to son
Microflora balance in pig stomach enteron aisle effectively prevents the generation of grice diarrhoea.From table 4, it can be seen that compound acidulant
F1, F2 can significantly reduce the quantity (P < 0.05) of Escherichia coli, to effectively prevent the generation of grice diarrhoea.
2.4 compound acidulants influence crude protein of weanling pig excrement etc.
5 compound acidulant of table influences crude protein of weanling pig excrement etc.
As can be seen from Table 5, F1 group energy, crude protein are substantially less than control group (P < 0.05), illustrate that F1 improves albumen
Utilization rate and S. E. A. promote the growth of pig；F2 group crude protein is substantially less than control group (P < 0.05), remaining is each
Not significant (the P of component difference>0.05).F1, F2 group promote the growth of pig, reduce energy and crude protein water in excrement
It is flat.
3 conclusions
From above-mentioned test result it can be concluded that, compound acidulant can in various degree promotion piglet growth, reduce enteron aisle
In Escherichia coli quantity, the generation of effective preventing post-weaning diarrhea, and test group I (F1) be better than test group II (F2).
Embodiment 2：A kind of preparation for the compound formulation reducing diarrhea of weaned piglets rate
The compound formulation is by oligosaccharide acidulant liquid and probiotics fermention liquid by volume 1:4 mix；
Wherein, oligosaccharide acidulant liquid be by embodiment 1 preferably go out compound acidulant formula 1 and oligosaccharide compounding and
At containing in the oligosaccharide acidulant liquid of every 1mL：3000 μ g of oxalic acid, 50 μ g of tartaric acid, 60 μ g of malic acid, 5000 μ g of lactic acid, lemon
Lemon acid 3000 μ g, glucose 0.1g, oligoisomaltose 0.05g, oligofructose 0.05g, chitosan oligomer 0.1g；
Glucose, oligoisomaltose, oligofructose and chitosan oligomer are commercial product.
Probiotics fermention liquid is by lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid and clostridium butyricum fermentation liquid by volume
Than 2:1 mixes.Lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid are by lactobacillus plantarum LP (Lactobacillus
Plantarum), Pediococcus pentosaceus PP (Pediococcus pentosaceus PP) co-fermentation obtains, which is documented in
An a kind of patent of invention " lactic acid bacteria semisolid fermentation product and technique for improving growth of animal performance " (CN of applicant
102599348 B) in, fermentation process is with " specific zymotechnique " step (1)-(4) in embodiment 3 in the patent, wherein pentose
Piece coccus PP (Pediococcus pentosaceus PP) is preserved in China typical culture collection center, and deposit number is
CCTCC NO：M 2012029；Lactobacillus plantarum LP (Lactobacillus plantarum) is documented in another hair of applicant
In bright patent " one plant of lactic acid bacteria that can be used for biological preservation and its application " (101914475 B of CN), it is preserved in Chinese allusion quotation
Type culture collection, deposit number are CCTCC NO：M 2010150.
Clostridium butyricum fermentation liquid is to ferment to obtain by clostridium butyricum (Clostridium butyrium) Cb-2, the butyric acid shuttle
Bacterium is documented in another patent of invention of applicant, and " one plant has the clostridium butyricum for the treatment of diarrhea effect and its replaces as sodium butyrate
In the application of Dai Pin " (102559550 B of CN), it is preserved in China typical culture collection center, deposit number is：CCTCC
NO：M 2011384；Fermentation process is the same as in embodiment 1 in the patent the step of " 4.6 prepare clostridium butyricum bacterium powder " part (i)-
(v)。
Specifically preparation process is：
By oxalic acid, tartaric acid, malic acid, lactic acid, citric acid, glucose, oligoisomaltose, oligofructose and oligomeric shell
Glycan mixing, uses water as solvent, heats and stir, make it completely dissolved, cool down, and adjusting pH is 3.0-5.0, and oligosaccharide is made
Acidulant liquid contains in the oligosaccharide acidulant liquid of every 1mL：3000 μ g of oxalic acid, 50 μ g of tartaric acid, 60 μ g of malic acid, lactic acid
5000 μ g, citric acid 3000 μ g, glucose 0.1g, oligoisomaltose 0.05g, oligofructose 0.05g, chitosan oligomer
0.1g；
By lactobacillus plantarum LP, Pediococcus pentosaceus PP mixed fermentation liquid and clostridium butyricum Cb-2 fermentation liquid by volume 2:1
Probiotics fermention liquid is made in mixing, and wherein lactobacillus plantarum LP viable count is 1 × 108Cfu/mL, Pediococcus pentosaceus PP viable count
It is 1 × 108Cfu/mL, clostridium butyricum Cb-2 viable count are 1 × 107cfu/mL；
By oligosaccharide acidulant liquid obtained and probiotics fermention liquid by volume 1:4 mixing to get.
Embodiment 3：Compound formulation prevents and treats diarrhea of weaned piglets verification test
1 materials and methods
1.1 experimental design
Test is carried out in drink township Ba Fubafu farm of Ningyang County of Shandong Province township, selects the same kind of 30-33 age in days, weight phase
Close weanling pig 90 is divided into control group, test group, Related product group, each group of 3 repetitions, 10 pigs of each repetition.It is right
According to the group clear water of drinking-water feeding daily, compound formulation (water dilutes 10 times) prepared by test group drinking-water feeding embodiment 2, Related product
The general raw edge (water dilutes 10 times) of group feeding, which is purchased from Henan Pu Nier Biology Pharmacy Co., Ltd.
1.2 test daily rations, management, evaluation, data analysis are referring to embodiment 1.
2 results and analysis
Influence of 2.1 compound formulations to diarrhea of weaned piglets rate
Influence of 6 compound formulation of table to diarrhea of weaned piglets rate
Diarrhea rate is an important indicator for evaluating diarrhea of weaned piglets.Death does not occur for this test whole process, tests
Group adds compound formulation of the invention by drinking-water to prevent and treat diarrhea of weaned piglets.As can be seen from Table 6, compared with the control group,
Test group can significantly reduce 27% (P of grice diarrhoea rate<0.05), Related product significantly reduces 11.34% (P of grice diarrhoea rate<
0.05).Compared with Related product group, test group significantly reduces 15.66% (P of grice diarrhoea rate<0.05), illustrate reducing wean
In terms of grice diarrhoea rate, test group effect is more excellent.
Influence of 2.2 compound formulations to Production Performance of Weaning Pigs
Influence of 7 compound formulation of table to Production Performance of Weaning Pigs
As can be seen from Table 7, compared with the control group, test group can significantly improve the average daily gain 36.4% of weanling pig
(P<0.05) 13.7% (P of feedstuff-meat ratio, is reduced<0.05)；Related product group difference is not significant (P > 0.05)；With Related product
Group is compared, and test group significantly improves 25% (P of average daily gain<0.05) 12% (P of feedstuff-meat ratio, is significantly reduced<0.05), illustrate to try
It is more excellent to test group effect in terms of improving Production Performance of Weaning Pigs.
Influence of 2.3 compound formulations to weanling pig faecal microbiota
Influence of 8 compound formulation of table to weanling pig faecal microbiota
As can be seen from Table 8, compared with the control group, lactic acid bacterium number dramatically increases (P in test group excrement<0.05), greatly
Enterobacteria quantity significantly reduces (P<0.05)；Related product group does not show lactic acid bacteria, the influence of Escherichia coli quantity in piglet excrement
It writes (P > 0.05)；Compared with Related product group, test group is remarkably promoting lactic acid bacteria intestinal colonisation, is inhibiting Escherichia coli breeding
More excellent (the P of aspect effect<0.05).
Influence of 2.4 compound formulations to weanling pig fecal energy, crude protein, crude fat
Influence of 9 compound formulation of table to weanling pig fecal energy, crude protein, crude fat
As can be seen from Table 9, compared with the control group, test group weanling pig fecal energy, crude fat significantly reduce (P<
0.05), the not significant (P of crude protein difference>0.05)；Related product group to weanling pig fecal energy, crude protein, crude fat shadow
Ring not significant (P>0.05)；Compared with Related product group, test group piglet fecal energy, crude fat significantly reduce (P<
0.05) digestive utilization ratio of feed, is improved.
Influence of 2.5 compound formulations to weanling pig excrement sIgA content
Influence of 10 compound formulation of table to weanling pig excrement sIgA content
As can be seen from Table 10, compared with the control group, test group excrement sIgA content significantly increases 20.8% (P<0.05),
Illustrate that test group can significantly improve piglet excrement sIgA content, promotes mucosa-immune, to improve the immune water of weanling pig
It is flat；Related product group influences not significant (P to sIgA content>0.05)；Test group sIgA content is significantly improved than Related product group
About 18.7% (P<0.05), in terms of enhancing mucosa-immune, test group effect is more preferable.
3 discuss
Influence of 3.1 compound formulations to diarrhea of weaned piglets rate
Grice diarrhoea is influenced by many factors.Diarrhea be due to enteropahtogenic microganism imbalance caused by, it is of the invention
Result of study discovery, the compound formulation that drinking-water addition is mixed by oligosaccharide, acidulant and probiotics can significantly reduce wean
Grice diarrhoea rate 27%, and Related product is only 11.34%, is reduced using the diarrhea rate after compound formulation than Related product group
15.66%, it is seen that the compound formulation of the invention better effect in terms of reducing diarrhea of weaned piglets rate.
Influence of 3.2 compound formulations to Production Performance of Weaning Pigs
The discovery of this result of study, compared with the control group, which significantly improves average daily gain 36.4%, reduces material
Meat is than 13.7%, and compared with Related product group, compound formulation significantly improves average daily gain 25%, reduces feedstuff-meat ratio 15.8%,
It can be seen that the compound formulation improves the production performance of weanling pig, it may be possible to which, since compound formulation has sour taste, stimulation is resolved
The appetite of milk piglet improves piglet daily gain, reduces the reason of feedstuff-meat ratio.
Influence of 3.3 compound formulations to weanling pig faecal microbiota
In the formula of compound formulation of the invention：Oligosaccharide, probiotics, acidulant can improve microbiota in enteron aisle
System, inhibits the growth of harmful bacteria, promotes the proliferation of beneficial bacterium.Firstly, acidulant can reduce the pH value of gastrointestinal tract, inhibit pathogen
Breeding regeneration；Secondly, the addition of probiotics can generate positive effect to the field planting of beneficial bacterium in enteron aisle；Finally, oligomeric
Sugar is in addition to it can supplement certain energy, moreover it is possible to which the feature for showing similar antibiotic inhibits the growth of a variety of pathogens.Three has
Effect reduces the quantity of pathogenic entero becteria, to ensure that the health of enteron aisle.Test also turns out that test group (drinks compound formulation group)
Microbiota in enteron aisle is improved, the growth of harmful bacteria is inhibited, promotes the proliferation of beneficial bacterium.
Influence of 3.4 compound formulations to weanling pig excrement trophic level
It to the utilization rate of feed is realized by many factors that the compound formulation, which can be improved piglet,.Firstly, reducing
Various enzymatic activitys in stomach can be improved in acidity；Secondly, the addition of probiotics also functions to certain promotion to the breeding of bacillus
Effect；Again, production acid, the bacteriostasis efficacy of clostridium butyricum are also beneficial to the utilization of feed；This test data shows test group excrement
Middle energy, fat are remarkably decreased, and illustrate that the addition of the compound formulation can improve efficiency of feed utilization.
Influence of 3.5 compound formulations to weanling pig excrement sIgA content
Secretory IgA, that is, sIgA is the immune main antibody of body mucous membrane local anti-infective.Therefore also known as mucous membrane local antibody,
The high and low energy of its content represents the level of mucosa-immune.A large number of experiments show that enterobacteriaceae can be adjusted after animal probiotic oral
Group makes gastrointestinal micro-flora be in optimal equilibrium state, activates associated lymphoid tissue in intestinal mucosa, makes sIgA antibody-secreting
Enhancing.The discovery of this test result, compared with control group, Related product group, test group compound formulation significantly improves sIgA content,
Illustrate that the compound formulation can enhance mucosa-immune power, improves body's immunity and premunition.
4 conclusions
Compound formulation of the invention can significantly reduce the diarrhea rate of weanling pig compared with control group, Related product group；
Piglet average daily gain is significantly improved, feedstuff-meat ratio is significantly reduced, improves the production performance of piglet；Dramatically increase lactic acid bacteria in excrement
Quantity reduces Escherichia coli quantity, balance intestinal flora；Weanling pig fecal energy and crude fat content are significantly reduced, is improved
Efficiency of feed utilization；Excrement sIgA content is significantly improved, mucosa-immune power is improved；Eventually by above-mentioned comprehensive function, this is compound
Preparation reduces the diarrhea rate of weanling pig.
Claims (6)
1. a kind of compound formulation for reducing diarrhea of weaned piglets rate, which is characterized in that be by oligosaccharide acidulant liquid and probiotics
Fermentation liquid by volume 1:(3-5) is mixed；
Contain in the oligosaccharide acidulant liquid of every 1mL：
Oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid 50-100 μ g, lactic acid 4000-5500 μ g, citric acid 2500-
4000 μ g, glucose 0.05-0.2g, oligoisomaltose 0.05-0.1g, oligofructose 0.02-0.06g, chitosan oligomer
0.08-0.15g；
The probiotics fermention liquid is by lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid and clostridium butyricum fermentation liquid by volume
Than (2-4):1 mixes；
It is CCTCC NO that the lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid, which are by deposit number,：The plant of M 2010150
Lactobacillus LP and deposit number are CCTCC NO：The Pediococcus pentosaceus PP co-fermentation of M 2012029 obtains；The clostridium butyricum
It is CCTCC NO that fermentation liquid, which is by deposit number,：The clostridium butyricum Cb-2 of M 2011384 ferments to obtain；
The compound formulation is prepared by the following method to obtain：
(1) by oxalic acid, tartaric acid, malic acid, lactic acid, citric acid, glucose, oligoisomaltose, oligofructose and oligomeric shell
Glycan mixing, uses water as solvent, heats and stir, make it completely dissolved, cool down, and adjusting pH is 3.0-5.0, and oligosaccharide is made
Acidulant liquid；
(2) by lactobacillus plantarum LP, Pediococcus pentosaceus PP mixed fermentation liquid and clostridium butyricum Cb-2 fermentation liquid (2- by volume
4):1 mixing, is made probiotics fermention liquid；
(3) by probiotics fermention liquid made from oligosaccharide acidulant liquid made from step (1) and step (2) by volume 1:(3-
5) mix to get.
2. a kind of compound formulation for reducing diarrhea of weaned piglets rate as described in claim 1, which is characterized in that the probiotics
Fermentation liquid is by lactobacillus plantarum, Pediococcus pentosaceus mixed fermentation liquid and clostridium butyricum fermentation liquid by volume 2:1 mixes.
3. a kind of compound formulation for reducing diarrhea of weaned piglets rate as described in claim 1, which is characterized in that the institute of every 1mL
It states and contains in oligosaccharide acidulant liquid：3000 μ g of oxalic acid, 50 μ g of tartaric acid, 60 μ g of malic acid, 5000 μ g of lactic acid, citric acid 3000
μ g, glucose 0.1g, oligoisomaltose 0.05g, oligofructose 0.05g, chitosan oligomer 0.1g.
4. a kind of compound formulation for reducing diarrhea of weaned piglets rate as described in claim 1, which is characterized in that the oligomeric shell
Glycan is polymerized by 2-10 glycosidic bond.
5. a kind of preparation method for the compound formulation for reducing diarrhea of weaned piglets rate, which is characterized in that steps are as follows：
(1) by oxalic acid, tartaric acid, malic acid, lactic acid, citric acid, glucose, oligoisomaltose, oligofructose and oligomeric shell
Glycan mixing, uses water as solvent, heats and stir, make it completely dissolved, cool down, and adjusting pH is 3.0-5.0, and oligosaccharide is made
Acidulant liquid contains in the oligosaccharide acidulant liquid of every 1mL：Oxalic acid 2500-3500 μ g, tartaric acid 30-60 μ g, malic acid 50-
100 μ g, lactic acid 4000-5500 μ g, citric acid 2500-4000 μ g, glucose 0.05-0.2g, oligoisomaltose 0.05-
0.1g, oligofructose 0.02-0.06g, chitosan oligomer 0.08-0.15g；
(2) by lactobacillus plantarum LP, Pediococcus pentosaceus PP mixed fermentation liquid and clostridium butyricum Cb-2 fermentation liquid (2- by volume
4):1 mixing, is made probiotics fermention liquid, wherein the viable count of lactobacillus plantarum LP, Pediococcus pentosaceus PP and clostridium butyricum Cb-2
Respectively greater than or equal to 1 × 108cfu/mL、1×108Cfu/mL and 1 × 107cfu/mL；The lactobacillus plantarum, pentose piece
It is CCTCC NO that coccus mixed fermentation liquid, which is by deposit number,：The lactobacillus plantarum LP and deposit number of M 2010150 is CCTCC
NO：The Pediococcus pentosaceus PP co-fermentation of M 2012029 obtains；It is CCTCC that the clostridium butyricum fermentation liquid, which is by deposit number,
NO：The clostridium butyricum Cb-2 of M 2011384 ferments to obtain；
(3) by probiotics fermention liquid made from oligosaccharide acidulant liquid made from step (1) and step (2) by volume 1:(3-
5) mix to get.
6. a kind of preparation method of compound formulation for reducing diarrhea of weaned piglets rate as claimed in claim 5, which is characterized in that
In step (3), oligosaccharide acidulant liquid and probiotics fermention liquid by volume 1:4 mixing.
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