CN100482104C - Process for preparing lactic acid bacteria oral liquor - Google Patents
Process for preparing lactic acid bacteria oral liquor Download PDFInfo
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- CN100482104C CN100482104C CNB2006100452911A CN200610045291A CN100482104C CN 100482104 C CN100482104 C CN 100482104C CN B2006100452911 A CNB2006100452911 A CN B2006100452911A CN 200610045291 A CN200610045291 A CN 200610045291A CN 100482104 C CN100482104 C CN 100482104C
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Abstract
The present invention relates to health food, and is especially preparation process of one kind of lactic acid bacteria oral liquid. The lactic acid bacteria oral liquid is prepared through mixing brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride in certain proportion, dissolving in water and heating to sterilize; inoculating one or several of Lactobacillus casei subsp. Casei, Lactobacillus plantarum, Lactobacillus acidophilus and Bacillus coagulans; culturing at 37deg.c for 48 hr; harvesting after the pH value is lowered to about 3.6; and bacteria-free packing. The lactic acid bacteria oral liquid is brown and sour and has live lactic acid bacteria content up to 1 billion/ml. It is used in improving digestive tract function and treating gastrointestinal tract ailments.
Description
(1) technical field
The present invention relates to a kind of preparation method of lactic acid bacteria oral liquor, belong to field of health care food.
(2) background technology
Along with deepening continuously of related science research, lactic acid bacteria is disclosed for the benefit of health more and more, as promote body to the microecological balance that absorbs, keeps gut flora of nutritional labeling, strengthen body's immunity etc.
Its major function is as follows:
1, regulates gut flora
Lactic acid bacteria has suppressed breeding and the field planting of pathogen by producing a large amount of organic acids, hydrogen peroxide, enzyme, bacteriocin etc., has promoted enterocinesia, keeps intestinal microecology and normal physiological function.Lactic acid bacteria is the profitable strain of settling down in enteron aisle, and the obligate anaerobe that with the lactic acid bacteria is representative is the main strength that constitutes the field planting drag.Lactic acid bacteria has also played the effect that harmful bacterium breeds that suppresses by pH value in the metabolism product acid reduction intestines.
2, strengthen immunity
Lactic acid bacteria is the phagocytosis of activating macrophage obviously, simultaneously can also activate bone-marrow-derived lymphocyte and NK cell, increase the generation of IgA in the enteron aisle, increase production of cytokines amounts such as IL-1, IL-2, IL-5, IL-6, TNF-α, stimulate human body to produce nospecific immunity; Because it can field planting in enteron aisle, be equivalent to natural active immunity on the other hand.They can also stimulate peritoneal macrophages in addition, produce interferon, promote cell division, produce antibody and cellular immunity etc., so can strengthen the non-specific and specific immune response of body, improve the resistance against diseases of body.When foreign matter is invaded body,, then have to strengthen body produces antibody to foreign matter effect by the immunocyte that lactic acid bacteria is activated.
Therefore, sour milk beverage has the health care of good enhancing immunity, adjusting gut flora.
Producing sour milk beverage at present mainly is to produce sour milk, with raw material be milk, bacterial classification is Lactobacillus plantarum, lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus casei, streptococcus fecalis, and also not utilizing brown sugar, glucose so far is that raw material prepares lactic acid bacteria oral liquor.
(3) summary of the invention
At the deficiencies in the prior art, the invention provides a kind of preparation method of new lactic acid bacteria oral liquor.
The preparation method of lactic acid bacteria oral liquor of the present invention, put it briefly and be, the present invention utilizes brown sugar, raw materials such as glucose, bacterial classification is selected from Lactobacillus casei cheese subspecies Lactobacillus casei subsp.Casei (bacterium numbering: CGMCC1.2435), Lactobacillus plantarum Lactobacillus plantarum (bacterium numbering: CGMCC1.103), lactobacillus acidophilus Lactobacillus acidophilus (bacterium numbering: CGMCC1.1854), (bacterium numbering: CGMCC1.2009) one of four kinds of probios or combination prepare lactic acid bacteria oral liquor by microbial fermentation to bacillus coagulans Bacilluscoagulans.Above-mentioned 4 kinds of probiotics strains are all available from Chinese common micro-organisms culture presevation administrative center.This product activity composition is one of above-mentioned 4 kinds of probios or combination.
The preparation method of lactic acid bacteria oral liquor of the present invention, step is as follows:
(1) by weight table sugar 1-10 part, edible glucose 1-10 part, peptone 0.1-2 part, magnesium sulfate 0.01-0.2 part, manganese sulfate 0.01-0.2 part, dipotassium hydrogen phosphate 0.01-1 part, sodium chloride 0.1-2 part are mixed, add water 75-98 part dissolving of purification, heat treatment method is sterilized, as culture medium;
(2) on above-mentioned culture medium, insert one of following bacterial classification or its combination, inoculum concentration is 0.5-2 part, weight portion: Lactobacillus casei cheese subspecies Lactobacillus casei subsp.Casei (bacterium numbering: CGMCC1.2435), Lactobacillus plantarum Lactobacillus plantarum (bacterium numbering: CGMCC1.103)
Lactobacillus acidophilus Lactobacillus acidophilus (bacterium numbering: CGMCC1.1854),
Bacillus coagulans Bacillus coagulans (bacterium numbering: CGMCC1.2009);
(3) the postvaccinal mixed liquor of step (2) was cultivated 48 hours at 37 ℃, the pH value gets final product when reducing to 3.4-4.0;
(4) product of step (3) is aseptic subpackaged, make lactic acid bacteria oral liquor.
Preferably, the described heat treatment of step (1) is to carry out under 110-128 ℃ temperature, and sterilization time is 25-180 minute.
Preferably, the described table sugar of step (1) is a brown sugar.
The lactic acid bacteria oral liquor of the inventive method preparation is a brown liquid, and flavor acid is about viable bacteria content 1,000,000,000/mL.Be specially adapted to improve indigestion, malabsorption, have no appetite, gastrointestinal problems such as diarrhoea, constipation, intestinal tympanites.
It is that raw material prepares lactic acid bacteria oral liquor that the present invention selects brown sugar, glucose first for use.When used Lactobacillus casei cheese subspecies are fermented separately, can in 48 hours, the pH value be dropped to 3.40, when Lactobacillus plantarum ferments separately, can in 48 hours, the pH value be dropped to 3.6, when lactobacillus acidophilus is fermented separately, can in 48 hours, the pH value be dropped to 3.9, when bacillus coagulans ferments separately, can in 48 hours, the pH value be dropped to 3.5.Four strain bacterium Mixed culture can drop to 3.6 with the pH value in 48 hours.The inventive method prepares lactic acid bacteria oral liquor, produces acid soon, probio content ideal.
(4) description of drawings
Fig. 1 is a Lactobacillus casei cheese subspecies single strain fermentation and acid curve, Fig. 2 is a Lactobacillus plantarum single strain fermentation and acid curve, Fig. 3 is a lactobacillus acidophilus single strain fermentation and acid curve, Fig. 4 is a bacillus coagulans single strain fermentation and acid curve, and the love song line is produced in Fig. 5 Lactobacillus casei cheese subspecies, Lactobacillus plantarum, lactobacillus acidophilus and four kinds of bacterial strain mixed culture fermentations of bacillus coagulans.Abscissa is fermentation time (unit: hour) among the figure, and ordinate is the pH value.
(5) specific embodiment
The present invention will be further described below in conjunction with embodiment, but be not limited thereto.The consumption unit of each component is weight portion among the embodiment 1-5.
Embodiment 1: Lactobacillus casei cheese subspecies single strain fermentation preparation lactic acid bacteria oral liquor
Present embodiment selects for use Lactobacillus casei cheese subspecies to ferment separately, can in 48 hours the pH value be dropped to about 3.6.The preparation method: with 2 parts in brown sugar, 5 parts of glucose, 0.2 part of peptone, 0.01 part in magnesium sulfate, 0.01 part of manganese sulfate, 0.01 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.2 part, add the running water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert 1 part of Lactobacillus casei cheese subspecies seed liquor then, cultivated 48 hours for 37 ℃, pH reduces to 3.6 o'clock results, bottling.Produce the love song line and see Fig. 1.The gained lactic acid bacteria oral liquor is a brown liquid, flavor acid, viable bacteria content 1,000,000,000/mL.
Embodiment 2: Lactobacillus plantarum single strain fermentation preparation lactic acid bacteria oral liquor
Present embodiment selects for use Lactobacillus plantarum to ferment separately, can in 40 hours the pH value be dropped to about 3.7.The preparation method: with 2 parts in brown sugar, 5 parts of glucose, 0.2 part of peptone, 0.01 part in magnesium sulfate, 0.01 part of manganese sulfate, 0.01 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.2 part, add the running water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert 1 part of Lactobacillus plantarum seed liquor then, cultivated 48 hours for 37 ℃, pH reduces to 3.6 o'clock results, bottling.Produce the love song line and see Fig. 2.The gained lactic acid bacteria oral liquor is a brown liquid, flavor acid, viable bacteria content 1,000,000,000/mL.
Embodiment 3: lactobacillus acidophilus single strain fermentation preparation lactic acid bacteria oral liquor
Present embodiment selects for use lactobacillus acidophilus to ferment separately, can in 30 hours the pH value be dropped to about 3.9.The preparation method: with 2 parts in brown sugar, 3 parts of glucose, 0.1 part of peptone, 0.02 part in magnesium sulfate, 0.02 part of manganese sulfate, 0.02 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.1 part, add the running water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert 1 part of lactobacillus acidophilus seed liquor then, cultivated 30 hours for 37 ℃, pH reduces to 3.9 o'clock results, bottling.Produce the love song line and see Fig. 3.The gained lactic acid bacteria oral liquor is a brown liquid, flavor acid, viable bacteria content 1,000,000,000/mL.
Embodiment 4: bacillus coagulans single strain fermentation preparation lactic acid bacteria oral liquor
Present embodiment selects for use bacillus coagulans to ferment separately, can in 37 hours the pH value be dropped to about 3.6.The preparation method: with 3 parts in brown sugar, 4 parts of glucose, 0.2 part of peptone, 0.02 part in magnesium sulfate, 0.02 part of manganese sulfate, 0.02 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.1 part, add the running water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert 1 part of bacillus coagulans seed liquor then, cultivated 37 hours for 37 ℃, pH reduces to 3.6 o'clock results, bottling.Produce the love song line and see Fig. 4.The gained lactic acid bacteria oral liquor is a brown liquid, flavor acid, viable bacteria content 1,000,000,000/mL.
Embodiment 5: Lactobacillus casei cheese subspecies, Lactobacillus plantarum, lactobacillus acidophilus, four kinds of probios of bacillus coagulans are pressed 1:1:1:1 volume ratio combined inoculation fermentation preparation lactic acid bacteria oral liquor.
Present embodiment selects for use Lactobacillus casei cheese subspecies, Lactobacillus plantarum, lactobacillus acidophilus, four kinds of probios of bacillus coagulans by 1:1:1:1 volume ratio combined inoculation fermentation, can in 28 hours the pH value be dropped to about 3.5.The preparation method: with 5 parts in brown sugar, 3 parts of glucose, 0.2 part of peptone, 0.02 part in magnesium sulfate, 0.01 part of manganese sulfate, 0.02 part of dipotassium hydrogen phosphate, sodium chloride mix for 0.1 part, add the running water dissolving through purifying, and 116 ℃ of logical steam are sterilized; Insert Lactobacillus casei cheese subspecies, Lactobacillus plantarum, lactobacillus acidophilus, four kinds of probios of bacillus coagulans then by totally 1 part of 1:1:1:1 volume ratio seed mixture liquid, cultivated 28 hours for 37 ℃, pH reduces to 3.5 o'clock results, bottling.Produce the love song line and see Fig. 5.The gained lactic acid bacteria oral liquor is a brown liquid, flavor acid, viable bacteria content 1,000,000,000/mL.
Embodiment 6:
For understanding lactic acid bacteria oral liquor, observed the 55 routine infants clinical therapeutic efficacy of suffering from diarrhoea autumn and winter to the curative effect that the infant suffers from diarrhoea autumn and winter.This group data is the diarrhoea sick child that Shandong hospital is in hospital, June at age～2 years old; Acute onset, the course of disease are in 3 days, and clinical symptoms is with heating, diarrhoea, abdominal distension or companion's mild dehydration, and diarrhoea number of times every day is more than 4 times; The stool proterties is egg soup sample or rare water sample, and routine inspection has fat globule or a little leucocyte, no red blood cell, and pyocyte, parasites etc. accompany or do not accompany the infection of the upper respiratory tract.
This group data is divided into 2 groups at random, and 30 examples are organized in treatment, male 20 examples, women 10 examples, age＜1 year old 16 examples; Control group 25 examples, male 15 examples, women 10 examples, age＜1 year old 12 examples.Two groups of case random samplings, sex, age, the course of disease, diarrhoea number of times, clinical symptoms are learned the processing there was no significant difference by statistics.The lactic acid bacteria oral liquor to embodiment 5 preparation is organized in treatment, dosage:＜1 years old every day 1 time, each 1 bottle (10mL); 2 bottles of 1～2 year old every days, divide 2 times oral.Serve on 3d, without antibiotic.Control group gives gentamicin and bismuth subcarbonate is oral, and gentamicin divides oral 2% gentamycin solution 3～4 times by 10mg/kg calculating every day; Bismuth subcarbonate＜1 years old 0.15g/ time, 3 times/day; 1～2 year old 0.2g/ time, 3 times/day; Be 3 days the course of treatment.Allly take medicine that sb.'s illness took a favorable turn but do not heal, then took medicine again 2～3 days.Two groups of whiles add according to the state of an illness uses ORS, and high hot person gives Physical temperature-lowering or drug cooling.
Efficacy assessment standard
Produce effects: the 24～48h that takes medicine, times of defecation be reduced to≤and 2 times/day or return to normal number of times, the stool proterties recovers normal, the clinical symptoms complete obiteration.Effectively: the 48～72h that takes medicine, times of defecation just are reduced to 2 times/day, and the stool proterties takes a turn for the better, and clinical symptoms disappears substantially.Invalid: as to take medicine back 72h diarrhoea number of times still〉2 times/day, or proterties do not have and is clearly better, even the state of an illness increases the weight of and the person of changing dressings.
Clinical effectiveness sees Table 1.
Table 1 liang group efficacy result relatively
Through x
2Check two groups of comparing differences significantly (P＜0.01)
The result shows that the treatment group is efficient and reaches 100%, and it is remarkable as seen to take the lactic acid bacteria oral liquor effect.
Embodiment 7:
The acute toxicity test of lactic acid bacteria oral liquor per os: this test is undertaken by HSPH of Shandong Medical University toxicologic study chamber.With feed 60 of Kunming mouses of lactic acid bacteria oral liquor stoste of the present invention, body weight 18-22g, male and female half and half.Mouse is divided into 6 groups at random, 10 every group, male and female half and half, behind the fasting 12h, per os is irritated stomach, and dosage is respectively 40mL/kg, 32mL/kg, 25.6mL/kg, 20.5mL/kg, 16.4mL/kg, 13.1mL/kg (r=0.8).Observed 14 days continuously after the mouse administration, record mouse poisoning symptom and death toll according to each group mouse death rate, are calculated LD with the Bliss method
50With 95% fiducial limit.The results are shown in Table 2.
Table 2 lactic acid bacteria oral liquor acute toxicity test in mice result
The chmice acute toxic reaction: per os is to the no abnormal phenomenon of mouse behind the animal subject.
The result shows that per os of mouse gives lactic acid bacteria oral liquor half lethal dose LD
50Greater than 40mL/kg.
Claims (2)
1, the preparation method of lactic acid bacteria oral liquor, step is as follows:
(1) by weight table sugar 1-10 part, edible glucose 1-10 part, peptone 0.1-2 part, magnesium sulfate 0.01-0.2 part, manganese sulfate 0.01-0.2 part, dipotassium hydrogen phosphate 0.01-1 part, sodium chloride 0.1-2 part are mixed, add water 74.6-97.8 part dissolving of purification, heat treatment method is sterilized, as culture medium; Described heat treatment is to carry out under 110-128 ℃ temperature, and sterilization time is 25-180 minute;
(2) mixing of following four bacterial classifications of access on above-mentioned culture medium, inoculum concentration is 0.5-2 part, weight portion: Lactobacillus casei cheese subspecies Lactobacillus casei subsp.Casei, bacterium numbering: CGMCC1.2435, Lactobacillus plantarum Lactobacillus plantarum, bacterium numbering: CGMCC1.103, lactobacillus acidophilus Lactobacillus acidophilus, bacterium numbering: CGMCC1.1854, bacillus coagulans Bacillus coagulans, bacterium numbering: CGMCC1.2009;
(3) the postvaccinal mixed liquor of step (2) was cultivated 48 hours at 37 ℃, the pH value gets final product when reducing to 3.4-4.0;
(4) product of step (3) is aseptic subpackaged, make lactic acid bacteria oral liquor.
2, the preparation method of lactic acid bacteria oral liquor as claimed in claim 1 is characterized in that the described table sugar of step (1) is a brown sugar.
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| JP5487379B2 (en) | 2008-03-19 | 2014-05-07 | 森下仁丹株式会社 | Blood phosphorus level increase inhibitor |
| CN101700061B (en) * | 2009-10-28 | 2012-06-27 | 山东康智多生物科技有限公司 | High-activity fermented milk beverage and preparation method thereof |
| CN103622026A (en) * | 2013-12-18 | 2014-03-12 | 青岛根源生物技术集团有限公司 | Nutritional oral solution containing probiotics and preparation method thereof |
| CN105831534B (en) * | 2016-03-28 | 2019-03-26 | 济南康多宝生物技术有限公司 | A kind of compound lactobacillus beverage and preparation method thereof |
| CN106858596A (en) * | 2017-02-10 | 2017-06-20 | 天津金匮堂生物科技有限公司 | A kind of probiotic acid jujube oral liquid and preparation method thereof |
| CN110464741A (en) * | 2019-02-25 | 2019-11-19 | 山东中微众康生物科技有限公司 | A kind of lactic acid bacteria oral liquor and preparation method thereof improving human body intestinal canal health |
| CN110463892A (en) * | 2019-02-25 | 2019-11-19 | 山东中微众康生物科技有限公司 | A kind of lactic acid bacteria solid beverage and preparation method thereof improving human body intestinal canal health |
| CN109700033B (en) * | 2019-03-04 | 2021-06-04 | 山东环亿生物科技有限公司 | Probiotic composition for regulating intestinal tract and preventing constipation and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1105544A (en) * | 1994-09-09 | 1995-07-26 | 扬州大学农学院 | Method for preparing active lactic acid bacteria oral liquor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1105544A (en) * | 1994-09-09 | 1995-07-26 | 扬州大学农学院 | Method for preparing active lactic acid bacteria oral liquor |
Non-Patent Citations (1)
| Title |
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| 新型符合乳酸菌制剂的初步研究. 万荣峰.硕士学位论文. 2005 * |
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