CN1305383C - Active bifidobacteria contained milk powder and its preparation method - Google Patents
Active bifidobacteria contained milk powder and its preparation method Download PDFInfo
- Publication number
- CN1305383C CN1305383C CNB2003101032336A CN200310103233A CN1305383C CN 1305383 C CN1305383 C CN 1305383C CN B2003101032336 A CNB2003101032336 A CN B2003101032336A CN 200310103233 A CN200310103233 A CN 200310103233A CN 1305383 C CN1305383 C CN 1305383C
- Authority
- CN
- China
- Prior art keywords
- bifidobacterium
- freeze
- milk powder
- vitamin
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a milk powder product containing active bifidobacteria and a preparation method thereof. The present invention adopts the freeze drying bacterium powder of bifidobacteria and isomaltose oligosaccharide as additives for producing the milk powder product with the health care functions of improving intestinal flora, enhancing immunity, etc. The living bacterium number of the bifidobacteria of the milk powder product of the present invention is larger than 10<7>cfu/ g, and the moisture content is smaller than 5%.
Description
[technical field]
The present invention relates to a kind of milk powder product that contains Bifidobacterium freeze-dried powder and isomalto-oligosaccharide and preparation method thereof.
[background technology]
Bifidobacterium (Bifidobacterium) is an important physical bacterium in the humans and animals enteron aisle, and having of it is beneficial to the gastral health status of raising humans and animals.Modern numerous studies show that: the alimentary canal microorganism is playing an important role aspect health and the disease, for example nutrition, physiological function, efficacy of drugs, cancer, immune response, aging etc. all closely related with archenteric flora.Bifidobacterium produces organic acid in metabolic process, the effect of inhibition enteron aisle saprophytic bacteria growth is arranged, thereby plays effects such as regulating gut flora, control diarrhoea, the vitamin metabolism of improving, raising immunity function, and health is had multinomial beneficial functional.
Bifidobacterium is large numbers of normal beneficial microbes in the human body intestinal canal, and human body is from the physiological change process in baby, child, teenager, youth, the prime of life to old age, and Bifidobacterium quantity reduces gradually in the intestines, and microbe species also has replacement and changes.When the people was dead, Bifidobacterium at first lost activity in the intestines.Under normal circumstances, Bifidobacterium and the peaceful coexistence of other floras, keep and adjust the microecological balance of human body together, make human body be in good health status, what all link closely with growth, metabolism and even the birth and old age, sickness and death of human body the Bifidobacterium content in the human body.Many paathogenic factors all can make the normal intestinal flora imbalance simultaneously, and cause long-term diarrhea, use the enteron aisle antibiotic invalid sometimes merely, and some cases also can increase the weight of the imbalance of gut flora.Therefore the quantity of replenishing and keep the active Bifidobacterium in the human body for improve the human immunologic function, improve vitamin metabolism, to strengthen health significant.
Modern age, how countries in the world all began one's study active Bifidobacterium as food additives, with the problem of manual method with this class viable bacteria food prepared therefrom.But because bifidobacterium growth condition harshness, its suitable growth temperature is 35～40 ℃, and the pH value is 6.5～7.0, and obligate anaerobic.Therefore to make the oral active Bifidobacterium can field planting and breeding in human body intestinal canal, must accomplish 2 points: the one, by the human stomach time, do not killed by hydrochloric acid in gastric juice; The 2nd, the active Bifidobacterium of sufficient amount be arranged, it improves the human immunologic function competence exertion, improves vitamin metabolism, strengthens the effect of health.The many restrictions of Bifidobacterium oral liquid in the market or food because of conditions such as culture process, processing methods, ubiquity survival bacterium amount is not high, shortcomings such as viable bacteria storage life weak point, thereby do not reach desirable health care purpose.
The present inventor finds, Bifidobacterium freeze-dried powder and isomalto-oligosaccharide will be added in the milk powder, can improve the ability of active Bifidobacterium field planting and breeding in human body intestinal canal, and keep sufficient amount, improve the human immunologic function, improve vitamin metabolism, strengthen the effect of health thereby really played.
[summary of the invention]
Technical problem to be solved
The object of the present invention is to provide a kind of milk powder product that contains Bifidobacterium freeze-dried powder and isomalto-oligosaccharide, be used to overcome the shortcoming of above-mentioned prior art.Milk powder product of the present invention has the human body intestinal canal flora of improvement, significantly improves the health care of body immunity.
Another purpose of the present invention provides a kind of preparation method of above-mentioned milk powder product.
A further object of the present invention provides a kind of Bifidobacterium freeze-dried powder that is used for milk powder product of the present invention and preparation method thereof.
Technical scheme
Specifically, the invention provides a kind of milk powder product that contains active Bifidobacterium, it is characterized in that it contains Bifidobacterium freeze-dried powder bacterium and isomalto-oligosaccharide, wherein, the milk powder product of per 10,000 parts of weight contains the Bifidobacterium freeze-dried powder of 0.1-50 part weight and the isomalto-oligosaccharide of 0.1-50 part weight.Preferably, the milk powder product of per 10,000 parts of weight contains the Bifidobacterium freeze-dried powder of 0.5-10 part weight and the isomalto-oligosaccharide of 0.5-10 part weight.
In preferred embodiments, the Bifidobacterium freeze-dried powder that is adopted in the milk powder product of the present invention can be one or more the freeze-dried powder of mixture in bifidobacterium infantis (Bifidobacterium infantis), bifidobacterium longum (Bifidobacterium longum), bifidobacterium bifidum (Bifidobacterium bifidum), bifidobacterium adolescentis (Bifidobacterium adolescentis), the bifidobacterium breve (Bifidobacterium breve).More preferably, the freeze-dried powder of bifidobacterium infantis (Bifidobacterium infantis) or bifidobacterium longum (Bifidobacterium longum).
In this article, isomalto-oligosaccharide is meant the isomalto-oligosaccharide that all are edible, and its molecular weight is generally 50,000-90, and 000, be preferably 80,000-90,000.
Isomalto-oligosaccharide also can adopt other similar oligosaccharides to substitute, and for example, adopts compound sugar such as FOS, galactooligosaccharide.
In specific embodiments, milk powder product for example of the present invention can adopt following concrete prescription: fresh milk 4000-6000 part, desalted whey powder 100-150 part, skimmed milk power 50-100 part, refining vegetable oil 40-60 part, sweetener 150-200 part, be selected from vitamin A, vitamin D, vitamin E, vitamin C, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, in the vitamin K, folic acid, biotin, pantothenic acid, nicotinic acid, choline one or more vitamin 1-2 part, be selected from one or more mineral matter 1-2 part, Bifidobacterium freeze-dried powder 0.5-3 part and the isomalto-oligosaccharide 0.5-3 part among Fe, Zn, Cu, I, the Mn.
Milk powder product preferably of the present invention contains component and proportioning is as follows: 5000 parts of fresh milks, 120 parts of desalted whey powders, 80 parts of skimmed milk powers, 50 parts of refining vegetable oils, 150 parts of sweeteners, 1 part of vitamin, 1 part in mineral matter, Bifidobacterium freeze-dried powder 1.5-3 part and isomalto-oligosaccharide 0.5-3 part.
Milk formula of the present invention also can suitably add some auxiliary material that improves mouthfeel or improve nutritional labeling, for example cream, honey, soybean lecithins etc. according to the needs of actual conditions.
In specific embodiments, for example the present invention's milk powder product of containing the Bifidobacterium freeze-dried powder can adopt following method preparation:
One, pretreatment of raw material: adopt centrifuge to raw milk for example fresh milk carry out clean newborn preliminary treatment, centrifuge speed is 6000g, takes the automatic or manual deslagging.
Two, batching homogeneous: add isomalto-oligosaccharide, the vitamin aqueous solution, mineral water solution and auxiliary material one by one to pretreated clean Ruzhong, carry out homogeneous with homogenizer then and handle, processing condition is 60～68 ℃ of temperature, pressure 15～22Mpa.
Three, sterilization: the emulsion behind the homogeneous is carried out sterilization, for example use the chip sterilizer 93-95 ℃ of following sterilization 5 minutes.
Four, concentrate: it is 40～45% that the emulsion after the sterilization is concentrated into emulsion concentration, is 40～45% with double-effect falling film evaporator concentrated emulsion concentration for example, and concentrated condition is that an effect evaporating temperature is 70～76 ℃, and two effect evaporating temperatures are 48～52 ℃.
Five, spray-drying: the emulsion after will concentrating is carried out drying, for example carries out spray-drying under atomisation pressure 120Mpa.
Six, cooling sieve powder: spray-dired powder is cooled to 35 ℃ rapidly sieves powder.
Seven, dried mixing: adopt dry powder blender that the Bifidobacterium freeze-dried powder for preparing is mixed in proportion with the milk powder that above-mentioned cooling sieve powder makes, get milk powder product of the present invention, wherein contain the bifidobacteria viable bacteria number greater than 10
7The cfu/ gram, moisture is less than 5%.
Eight, weighing and bagging: with the milk powder weighing packing that contains Bifidobacterium of above-mentioned steps seven preparations, nitrogen-filled packaging promptly makes milk powder product finished product of the present invention.
The Bifidobacterium freeze-dried powder that adopts in the milk powder product of the present invention is preferably by following method preparation:
(1) actication of culture: select for use to have the Bifidobacterium of improving gut flora and improving immunity, the preferred bifidobacterium infantis (Bifidobacterium infantis) that adopts, more preferably for example strain number is BI 002, depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.0997, preservation date is the bifidobacterium infantis (Bifidobacterium infantis) on September 1st, 2003, or be preferably bifidobacterium longum (Bifidobacterium longum), more preferably for example strain number is BL 003, depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.0998, and preservation date is to be bifidobacterium longum (Bifidobacterium longum) on September 1st, 2003.
The bacterial classification that low temperature is preserved was at room temperature placed for example 12-36 hour, preferred 24 hours, add in the glass-tube at the aseptic 0.2mL fluid nutrient medium 1 that for example takes a morsel of desinfection chamber afterwards, get bacterial classification inoculation on fluid nutrient medium 1 and agar plate, anaerobism was cultivated 48～72 hours under the 35-37 ℃ of condition, activate the three generations more continuously, to improve the bacterial strain vigor, as female culture.
Wherein used culture medium 1 is the TPY culture medium, it is by pancreatin hydrolysis casein 12g, many peptones 3g, soy peptone 5g, glucose 5g, FOS 5g, dusty yeast 5g, Tween-80 1g, liver infusion 200mL, mixing salt solution 10mL, the 100mL of Tomato juice and L-cysteine hydrochloric acid 0.5g, adding distil water 675mL is 7.2 at pH again, temperature makes for sterilization under 115 ℃ the condition for example for example adds mixed vitamin after 20 minutes, and wherein mixing salt solution is by K
2HPO
420.0g, MgCl
26H
2O5.0g, ZnSO
47H
2O2.5g, CaCl
21.5g and FeCl
30.5g adding distil water 100mL makes, mixed vitamin is by Cobastab
1100mg, Cobastab
2100mg, Cobastab
6100mg, sodium pantothenate 10mg and biotin 1mg adding distil water 100mL filter sterilization and make.
(2) preparation of one-level culture: female culture is inserted culture medium 1 (adopt with above-mentioned steps in identical culture medium 1), and connecing the bacterium amount is 2～3%, and anaerobism was cultivated 10-16 individual hour under 35～37 ℃ of conditions, as the one-level culture.
(3) preparation of secondary culture: the one-level culture is inserted culture medium 2, and connecing the bacterium amount is 2～3%, and anaerobism was cultivated 10-14 hour under 37 ℃ of conditions, as the secondary culture.
Wherein used culture medium 2 is to be 7.2 by soy peptone 16.67g, casein peptone 8.33g, lactose 5.0～10.0g, yeast extract 5.0g, L-cysteine hydrochloric acid 0.5g, compound sugar 5～7g, Tween-80 1.0g, mixing salt solution 5mL, liver infusion or carrot juice 150mL and water 845mL at pH, temperature is that sterilization made in 20 minutes under 115 ℃ the condition, and wherein mixing salt solution is by K
2HPO
420.0g, MgCl
26H
2O5.0g, ZnSO
47H
2O2.5g, CaCl
21.5g, FeCl
30.5g and distilled water 100mL forms.
When selecting for use bacterial classification to be bifidobacterium infantis, preferably use liver infusion in above-mentioned culture medium 2 components; When selecting for use bacterial classification to be bifidobacterium longum, preferably use carrot juice in above-mentioned culture medium 2 components.
(4) increasing bacterium cultivates: the secondary culture is inserted culture medium 2 (adopt with above-mentioned steps in identical culture medium 2), connecing the bacterium amount is 2～3%, in temperature is 35～37 ℃, the Ph value is 7.0～7.2, under the stirring condition, for example mixing speed is at 300-500rpm, and fermenting obtained bifidus bacillus fermented liquid after 8～12 hours.
(5) centrifugation: with centrifugal 15～20 minutes of above-mentioned bifidus bacillus fermented liquid, for example adopt centrifugal rotational speed 6000～8000rpm under 4 ℃ of conditions, it is standby to take off layer precipitation.
(6) add the freeze drying protectant freeze-drying: will precipitate with after freeze drying protectant fully mixes; at vacuum 6Pa; cryogenic temperature-40 ℃; freeze-drying time is that freeze-drying can obtain the Bifidobacterium freeze-dried powder under 7～10 hours the condition; wherein the consumption of freeze drying protectant is 1/4～1/2 of a fermentating liquid volume, preferably adopts 1/3.
Described freeze drying protectant is made up of skimmed milk powder 100g, glycerine 10g, FOS 25g, lactose 12.5g, cystine 1g, vitamin C 3g, water 1000g.Wherein cystine, vitamin C adopt filtration sterilization, and other compositions adopt 105 ℃ of sterilizations 30 minutes.
The Bifidobacterium freeze-dried powder that adopts in the milk powder product of the present invention is preferably by method for preparing.The Bifidobacterium freeze-dried powder that adopts said method to make contains the bifidobacteria viable bacteria number greater than 10 in wherein every gram Bifidobacterium freeze-dried powder
10Cfu, moisture is less than 5%.
Bifidobacterium freeze-dried powder product of the present invention also can eat separately except that can be used as the milk flour additive agent, also can be used as food additives and is applied in the production of Foods or drinks.For example can directly mix in proportion with edible pulvis such as bean powder, ground rice, milk powder, pollen, medicinal powder as health food.Perhaps mix in proportion with liquid beverages such as vegetable juice or fruit juice, and add additive such as an amount of sweetener as required, the beverage that contains active Bifidobacterium is made in the back can that stirs.
Beneficial effect
Milk powder product of the present invention contains isomalto-oligosaccharide and Bifidobacterium freeze-dried powder, and every gram milk powder contains the bifidobacteria viable bacteria number greater than 10
7Cfu, moisture is less than 5%, and isomalto-oligosaccharide wherein is the best MF of Bifidobacterium, can promote that Bifidobacterium breeds in a large number in the human body intestinal canal, increases the ratio of profitable strain, suppresses growing of pathogen and spoilage organisms.The isomalto-oligosaccharide sugariness is low, is not decomposed by harmful bacterium and utilizes, and is not absorbed by gastro-intestinal digestion, can not increase too many heat, pure taste.Isomalto-oligosaccharide has the effect that improves immune response ability and a large amount of multiplication capacities of Bifidobacterium.Therefore the active Bifidobacterium in the milk powder product of the present invention can be in human body intestinal canal can field planting and breeding, and keep sufficient amount, thereby really played and to have improved the human immunologic function, improve vitamin metabolism, strengthen the effect of health, it is simple to have production technology in addition, advantages such as the good and nutritive value height of product taste have good market prospects and economic benefit.
The every gram of Bifidobacterium freeze-dried powder of the present invention contains the bifidobacteria viable bacteria number greater than 10
10Cfu, moisture is less than 5%, and advantage such as it is simple to have production technology in addition, is of high nutritive value, of many uses can eat separately, also can be used as food additives, has good market prospects and economic benefit.
[description of drawings]
Fig. 1 contains the preparation process figure of Bifidobacterium milk powder for the present invention.
Fig. 2 is the preparation process figure of Bifidobacterium freeze-dried powder of the present invention.
[specific embodiment]
Embodiment 1: the preparation of Bifidobacterium freeze-dried powder
A) actication of culture: with the bifidobacterium infantis (Bifidobacteriuminfantis of low temperature preservation, strain number is BI 002, depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC No.0997, preservation date is on September 1st, 2003) at room temperature placed 24 hours, at desinfection chamber the effective 70% alcohol swab wipe surfaces of bacterial classification peace bottle is sterilized, with alcolhol burner that its top is roasting hot then, drip cold sterile purified water, make the glass-tube cracking, knock out top with tweezers, the aseptic 0.2mL fluid nutrient medium (1) of getting adds in the glass-tube, gets fungus with sterilization platinum ear insertion glass-tube and is inoculated on fluid nutrient medium (1) and the agar plate, anaerobism was cultivated 60 hours under the 35-37 ℃ of condition, activate the three generations more continuously, to improve the bacterial strain vigor, as female culture.Wherein used culture medium (1) is the TPY culture medium, it is by pancreatin hydrolysis casein 12g, many peptones 3g, soy peptone 5g, glucose 5g, FOS 5g, dusty yeast 5g, Tween-80 1g, liver infusion 200mL, mixing salt solution 10mL, the 100mL of Tomato juice and L-cysteine hydrochloric acid 0.5g, adding distil water 675mL is 7.2 at pH again, temperature is that sterilization adds mixed vitamin and makes under 115 ℃ the condition after 20 minutes, and wherein mixing salt solution is by K
2HPO
420.0g, MgCl
26H
2O5.0g, ZnSO
47H
2O2.5g, CaCl
21.5g and FeCl
30.5g adding distil water 100mL makes, mixed vitamin is by Cobastab
1100mg, Cobastab
2100mg, Cobastab
6100mg, sodium pantothenate 10mg and biotin 1mg adding distil water 100mL filter sterilization and make.
B) preparation of one-level culture: female culture is inserted culture medium (1) (adopt with above-mentioned steps in identical culture medium (1)), and connecing the bacterium amount is 2.5%, and the anaerobism cultivation is 14 hours under 35～37 ℃ of conditions, as the one-level culture.
C) preparation of secondary culture: the one-level culture is inserted culture medium (2), and connecing the bacterium amount is 2.5%, and anaerobism was cultivated 12 hours under 35～37 ℃ of conditions, as the secondary culture.Wherein used culture medium (2) is to be 7.2 by soy peptone 16.67g, casein peptone 8.33g, lactose 5.0～10.0g, yeast extract 5.0g, L-cysteine hydrochloric acid 0.5g, compound sugar 5～7g, Tween-80 1.0g, mixing salt solution 5mL, carrot juice 150mL and water 845mL at pH, temperature is that sterilization made in 20 minutes under 115 ℃ the condition, and wherein mixing salt solution is by K
2HPO
420.0g, MgCl
26H
2O5.0g, ZnSO
47H
2O2.5g, CaCl
21.5g, FeCl
30.5g and distilled water 100mL forms.
D) increasing bacterium cultivates: the secondary culture is inserted culture medium (2) (adopt with above-mentioned steps in identical culture medium (2)), connecing the bacterium amount is 2.5%, in temperature is 35～37 ℃, and the Ph value is 7.1, and mixing speed obtains bifidus bacillus fermented liquid at the condition bottom fermentation of 400rpm after 10 hours;
E) centrifugation: at 4 ℃, with centrifugal 15 minutes of above-mentioned bifidus bacillus fermented liquid, it was standby to take off layer precipitation under the condition of centrifugal rotational speed 7000rpm;
F) add the freeze drying protectant freeze-drying: will precipitate with after freeze drying protectant fully mixes; at vacuum 6Pa; cryogenic temperature-40 ℃, freeze-drying time are that freeze-drying can obtain the bifidobacterium infantis freeze-dried powder under 9 hours the condition, and wherein the consumption of freeze drying protectant is 1/3 of a fermentating liquid volume.Described freeze drying protectant is made up of skimmed milk powder 100g, glycerine 10g, FOS 25g, lactose 12.5g, cystine 1g, vitamin C 3g, water 1000g.Wherein cystine, vitamin C adopt filtration sterilization, and other compositions adopt 105 ℃ of sterilizations 30 minutes, get the bifidobacterium infantis freeze-dried powder.
Adopt above-mentioned similar step, selecting strain number for use is BL 003, depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCCNo.0998, and preservation date is that the bifidobacterium longum (Bifidobacteriumlongum) on September 1st, 2003 is as active Bifidobacterium; Adopt carrot juice to substitute liver infusion in culture medium 2 components, prepare the bifidobacterium longum freeze-dried powder.
Embodiment 2: the preparation of milk powder product of the present invention
(1) pretreatment of raw material: adopt centrifuge that 5000 parts of raw milk fresh milks are carried out clean newborn preliminary treatment, centrifuge speed is 6000rpm, takes the automatic or manual deslagging.
(2) batching homogeneous: add 0.4 part of 1.5 parts of isomalto-oligosaccharides, 1 part of multivitamin aqueous solution, 1 part of the composite mineral matter aqueous solution, 120 parts of desalted whey powders, 80 parts of skimmed milk powers, 50 parts of refining vegetable oils, 150 parts of sweetener glucose, 20 parts in cream, 10 parts of honey, 1 part of soybean lecithin and taurine one by one to pretreated clean Ruzhong; about 65 ℃ of temperature, carry out homogeneous with homogenizer under the pressure 30Mpa and handle then.
Wherein B B-complex is by vitamin A, vitamin D, vitamin E, vitamin C, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, vitamin K, folic acid, biotin, pantothenic acid, nicotinic acid, choline form.Composite mineral matter is made up of mineral matter Fe, Zn, Cu, I, Mn.
(3) sterilization: the emulsion behind the homogeneous is used chip sterilizer sterilization 5 minutes down at 93～95 ℃.
(4) concentrate: it is 43% that the emulsion after the sterilization is concentrated into emulsion concentration with double-effect falling film evaporator, and concentrated condition is that an effect evaporating temperature is about 73 ℃, and two effect evaporating temperatures are about 50 ℃.
(5) spray-drying: the emulsion after concentrating is to carry out spray-drying under the condition of 120Mpa at pressure.
(6) cooling sieve powder: the powder that sprays during with spray-drying is cooled to 35 ℃ rapidly and sieves powder.
(7) do to mix: adopt dry powder blender with bifidobacterium infantis (Bifidobacteriuminfantis) freeze-dried powder for example 1.5 portions of milk powder that make with above-mentioned steps (6) of freeze-dried powder of embodiment 1 preparation mix, contain the bifidobacteria viable bacteria number greater than 10 in mixed every gram milk powder
7Cfu, moisture is less than 5%.
(8) weighing and bagging: with above-mentioned steps (7) preparation contain Bifidobacterium milk powder weighing packing, nitrogen-filled packaging promptly makes milk powder product finished product of the present invention.
Embodiment 3: the preparation of milk powder product of the present invention
(1) pretreatment of raw material: adopt centrifuge that 5000 parts of raw milk fresh milks are carried out clean newborn preliminary treatment, centrifuge speed is 6000rpm, takes the automatic or manual deslagging.
(2) batching homogeneous: add 0.4 part of 3 parts of isomalto-oligosaccharides, 1 part of multivitamin aqueous solution, 1 part of the composite mineral matter aqueous solution, 120 parts of desalted whey powders, 80 parts of skimmed milk powers, 50 parts of refining vegetable oils, 150 parts of sweetener glucose, 20 parts in cream, 10 parts of honey, 1 part of soybean lecithin and taurine one by one to pretreated clean Ruzhong; about 65 ℃ of temperature, carry out homogeneous with homogenizer under the pressure 30Mpa and handle then.
Wherein B B-complex is by vitamin A, vitamin D, vitamin E, vitamin C, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, vitamin K, folic acid, biotin, pantothenic acid, nicotinic acid, choline form.Composite mineral matter is made up of mineral matter Fe, Zn, Cu, I, Mn.
(3) sterilization: the emulsion behind the homogeneous is used chip sterilizer sterilization 5 minutes down at 93～95 ℃.
(4) concentrate: it is 43% that the emulsion after the sterilization is concentrated into emulsion concentration with double-effect falling film evaporator, and concentrated condition is that an effect evaporating temperature is about 73 ℃, and two effect evaporating temperatures are about 50 ℃.
(5) spray-drying: the emulsion after concentrating is to carry out spray-drying under the condition of 120Mpa at pressure.
(6) cooling sieve powder: the powder that sprays during with spray-drying is cooled to 35 ℃ rapidly and sieves powder.
(7) do to mix: adopt dry powder blender for example to adopt 3 parts of the freeze-dried powders of the method preparation that is similar to embodiment 1 to mix bifidobacterium longum (Bifidobacteriumlongum) freeze-dried powder, contain the bifidobacteria viable bacteria number greater than 10 in mixed every gram milk powder with above-mentioned milk powder
7Cfu, moisture is less than 5%.
(8) weighing and bagging: with above-mentioned steps (7) preparation contain Bifidobacterium milk powder weighing packing, nitrogen-filled packaging promptly makes milk powder product finished product of the present invention.
Test example 1: Bifidobacterium freeze-dried powder count plate test of the present invention
Count plate test specimen: 1g is according to the Bifidobacterium freeze-dried powder of embodiment 1 preparation.
The count plate test method:
Above-mentioned sample is carried out the dilution of successively decreasing of 10 times of gradients with dilution, get suitable dilution and topple over the BS flat board, 37 ℃ of anaerobism are cultivated after 36 hours and are counted viable count, are being scaled original bacterium number.
Wherein dilution is by Na
2HPO
46g, KH
2PO
44.5g, L-cysteine hydrochloric acid 0.5g, agar 1g, Tween-80 0.5g, adding distil water is to 1000mL, 115 ℃ down sterilization made in 20 minutes;
The BS culture medium is made up of the 200mL of Tomato juice, peptone 7g, dusty yeast 6g, pancreatin hydrolysis casein 5g, phytone 3g, many peptones 3g, glucose 10g, soluble starch 0.5g, Tween 80 1g, solution A 10mL, solution B 5mL, agar 20g.The solution A that the BS culture medium is used is by KH
2PO
425g, K
2HPO
425g, water 250g form; Solution B is by MgSO
47H
2O10g, FeSO
47H
2O0.5g, NaCl0.5g, MnSO
40.337g water 250g forms; The BS annex solution is added in the sterilization flask by sodium propionate 30g, adds sterile purified water 80mL, waits to dissolve 400 milligrams of after vulcanization acid neomycins, 100 milligrams of paromomycin, and LiCl6g adds sterile purified water again and makes to 100mL.
The count plate result of the test: sample contains the bifidobacteria viable bacteria number greater than 10
10Cfu/g.
Test example 2: milk powder product Bifidobacterium survival test of the present invention
Test specimen: the milk powder that adopts the embodiment of the invention 2.
Test method: in culture medium, add natrii tauroglycocholas by 0.3%, 0.5%, 2% amount respectively, connect bacterium by 10% and measure aseptic access 10
9Cfu/mL bacterium liquid, 37 ℃ of anaerobic condition effects down, respectively at 0,1.5,3 hour sampling and measuring viable count, distilled water compared.
Result of the test: the present invention is as follows with the survival condition of bacterium: bifidobacterium longum acts on 12 hours under 2% gallbladder salinity condition, survival rate is 13.96%; Under the bifidobacterium infantis 2% gallbladder salinity condition, still have growth tendency.
Test example 3: milk powder product health-care effect test of the present invention
Test specimen: the milk powder that adopts the embodiment of the invention 2 and embodiment 3.
Test method: adopt the BALB/C small white mouse to test, divide control group, bifidobacterium infantis group and bifidobacterium longum group, every day, every mouse was irritated stomach Bifidobacterium milk powder, and control group is irritated stomach sterile distilled water, coprocessing 30 days.
Irritating the stomach small white mouse by milk powder product of the present invention finds: bifidobacterium infantis milk powder and bifidobacterium longum milk powder can significantly increase the content (p＜0.05) of IgA in the blood, IgG, C3, C4, IL-2, IL-6 and IL-8, under the number of viable that is equal to mutually, bifidobacterium infantis milk powder effect is better than bifidobacterium longum milk powder; Bifidobacterium infantis milk powder can extremely significantly increase the content (p＜0.01) of IgM.Two bacterial strains all can significantly increase the quantity of small white mouse intestinal bifidobacteria (p＜0.05) and lactobacillus, significantly reduce the quantity (p＜0.05) of enterobacteria.
Though the present invention is described in detail above to have used general explanation and implementer's case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, not departing from the these modifications or improvements basically of spirit of the present invention, all belong to the scope of protection of present invention.
Claims (7)
1. milk powder product that contains the Bifidobacterium freeze-dried powder, it is characterized in that per 10, the milk powder product of 000 part of weight contains the Bifidobacterium freeze-dried powder of 0.1-50 part weight and the isomalto-oligosaccharide of 0.1-50 part weight, and wherein, the Bifidobacterium freeze-dried powder makes as follows:
(1) actication of culture: select for use to have the freeze-drying lactobacillus that improves gut flora and improve immunity, the bacterial classification that low temperature is preserved was at room temperature placed 12-36 hour, add in the glass-tube at the aseptic fluid nutrient medium 1 of getting of desinfection chamber, get bacterial classification inoculation on fluid nutrient medium 1 and agar plate, anaerobism was cultivated 48～72 hours under the 35-37 ℃ of condition, activate the three generations more continuously, as female culture;
(2) preparation of one-level culture: female culture is inserted culture medium 1, connecing the bacterium amount is 2～3%, anaerobism was cultivated 10-16 hour under 35～37 ℃ of conditions, as the one-level culture, culture medium 1 is the TPY culture medium, it is by pancreatin hydrolysis casein 12g, many peptones 3g, soy peptone 5g, glucose 5g, FOS 5g, dusty yeast 5g, Tween-80 1g, liver infusion 200mL, mixing salt solution 10mL, 100mL of Tomato juice and L-cysteine hydrochloric acid 0.5g, adding distil water 675mL is 7.2 at pH again, temperature is to sterilize under 115 ℃ the condition, add mixed vitamin after 20 minutes and make, wherein mixing salt solution is by K
2HPO
420.0g, MgCl
26H
2O5.0g, ZnSO
47H
2O2.5g, CaCl
21.5g and FeCl
30.5g adding distil water 100mL makes, mixed vitamin is by Cobastab
1100mg, Cobastab
2100mg, Cobastab
6100mg, sodium pantothenate 10mg and biotin 1mg adding distil water 100mL filter sterilization and make;
(3) preparation of secondary culture: the one-level culture is inserted culture medium 2, connecing the bacterium amount is 2～3%, anaerobism was cultivated 10-14 hour under 35～37 ℃ of conditions, as the secondary culture, culture medium 2 is to be 7.2 by soy peptone 16.67g, casein peptone 8.33g, lactose 5.0～10.0g, yeast extract 5.0g, L-cysteine hydrochloric acid 0.5g, compound sugar 5～7g, Tween-80 1.0g, mixing salt solution 5mL, liver infusion or carrot juice 150mL and water 845mL at pH, temperature is that sterilization made in 20 minutes under 115 ℃ the condition, and wherein mixing salt solution is by K
2HPO
420.0g, MgCl
26H
2O5.0g, ZnSO
47H
2O2.5g, CaCl
21.5g, FeCl
30.5g and distilled water 100mL forms;
(4) increasing bacterium cultivates: the secondary culture is inserted culture medium 2, and connecing the bacterium amount is 2～3%, is 35～37 ℃ in temperature, and the Ph value obtains bifidus bacillus fermented liquid at the stirring condition bottom fermentation after 8～12 hours 7.0～7.2;
(5) centrifugation: with centrifugal 15～20 minutes of above-mentioned bifidus bacillus fermented liquid, it was standby to take off layer precipitation under 4 ℃ of conditions;
(6) add the freeze drying protectant freeze-drying: will precipitate with after freeze drying protectant fully mixes; at vacuum 6Pa; cryogenic temperature-40 ℃; freeze-drying time is that freeze-drying can obtain the Bifidobacterium freeze-dried powder under 7～10 hours the condition; wherein the consumption of freeze drying protectant is 1/4～1/2 of a fermentating liquid volume; freeze drying protectant is made up of skimmed milk powder 100g, glycerine 10g, FOS 25g, lactose 12.5g, cystine 1g, vitamin C 3g, water 1000g; wherein cystine, vitamin C adopt filtration sterilization, and other compositions adopt 105 ℃ of sterilizations 30 minutes.
2. milk powder product according to claim 1, wherein, the milk powder product of per 10,000 parts of weight contains the Bifidobacterium freeze-dried powder of 0.5-10 part weight and the isomalto-oligosaccharide of 0.5-10 part weight.
3. according to arbitrary milk powder product of claim 1-2, wherein, the isomalto-oligosaccharide molecular weight is 80,000-90,000.
4. milk powder product according to claim 1, wherein, described Bifidobacterium freeze-dried powder is one or more the freeze-dried powder of mixture in bifidobacterium infantis (Bifidobacterium infantis), bifidobacterium longum (Bifidobacterium longum), bifidobacterium bifidum (Bifidobacterium bifidum), bifidobacterium adolescentis (Bifidobacterium adolescentis), the bifidobacterium breve (Bifidobacterium breve).
5. according to the milk powder product of claim 4, wherein, described Bifidobacterium freeze-dried powder is the freeze-dried powder of bifidobacterium infantis (Bifidobacterium infantis) or bifidobacterium longum (Bifidobacterium longum).
6. according to claim 4 or 5 described milk powder products, its raw material comprises:
Fresh milk 4000-6000 part, desalted whey powder 100-150 part, skimmed milk power 50-100 part, refining vegetable oil 40-60 part, sweetener 150-200 part, be selected from vitamin A, vitamin D, vitamin E, vitamin C, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, in the vitamin K, folic acid, biotin, pantothenic acid, nicotinic acid, choline one or more vitamin 1-2 part, be selected from one or more mineral matter 1-2 part, Bifidobacterium freeze-dried powder 0.5-3 part and the isomalto-oligosaccharide 0.5-3 part among Fe, Zn, Cu, I, the Mn.
7. the preparation method of a milk powder product as claimed in claim 1 is characterized in that it comprises following preparation process:
(1) adopt centrifuge that raw milk is carried out clean newborn preliminary treatment;
(2) add isomalto-oligosaccharide, the vitamin aqueous solution, mineral water solution and auxiliary material one by one to pretreated clean Ruzhong, carry out homogeneous with homogenizer then and handle;
(3) emulsion behind the homogeneous is used the sterilization of chip sterilizer;
(4) emulsion after the sterilization being concentrated into emulsion concentration with double-effect falling film evaporator is 40～45%, and concentrated condition is that an effect evaporating temperature is 70～76 ℃, and two effect evaporating temperatures are 48～52 ℃;
(5) emulsion after concentrating is carried out spray-drying;
(6) powder of drying is cooled to 35 ℃ rapidly and sieves powder;
(7) adopt dry powder blender that the Bifidobacterium freeze-dried powder of preparation is mixed with the milk powder that above-mentioned steps (6) makes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101032336A CN1305383C (en) | 2003-11-03 | 2003-11-03 | Active bifidobacteria contained milk powder and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2003101032336A CN1305383C (en) | 2003-11-03 | 2003-11-03 | Active bifidobacteria contained milk powder and its preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1602708A CN1602708A (en) | 2005-04-06 |
| CN1305383C true CN1305383C (en) | 2007-03-21 |
Family
ID=34661357
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2003101032336A Expired - Fee Related CN1305383C (en) | 2003-11-03 | 2003-11-03 | Active bifidobacteria contained milk powder and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1305383C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1776877A1 (en) * | 2005-10-21 | 2007-04-25 | N.V. Nutricia | Method for stimulating the intestinal flora |
| CN101449830B (en) * | 2008-12-29 | 2012-01-11 | 山东龙力生物科技股份有限公司 | Mid-aged milk powder added with biostime and preparation method thereof |
| CN102144670A (en) * | 2011-02-28 | 2011-08-10 | 澳优乳业（中国）有限公司 | Milk powder with probiotic formula and preparation method thereof |
| CN102293362A (en) * | 2011-07-15 | 2011-12-28 | 姚东霞 | Total nutrient infant formula farina |
| CN102356778B (en) * | 2011-09-27 | 2013-10-30 | 内蒙古伊利实业集团股份有限公司 | Sea-buckthorn nutrient milk powder and preparation method thereof |
| CN102948483B (en) * | 2012-12-03 | 2014-01-15 | 内蒙古伊利实业集团股份有限公司 | Blood fat reducing milk powder and preparation method thereof |
| CN103039611A (en) * | 2012-12-20 | 2013-04-17 | 内蒙古伊利实业集团股份有限公司 | Powdered milk for preventing AD (Alzheimer's disease) |
| CN104824166A (en) * | 2015-05-08 | 2015-08-12 | 陕西雅泰乳业有限公司 | Cow/sheep milk powder containing lactobacillus and preparation method thereof |
| CN108018243B (en) * | 2018-01-08 | 2020-10-16 | 金华银河生物科技有限公司 | Lactobacillus plantarum and composite probiotic wolfberry fruit product and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107656A (en) * | 1994-04-05 | 1995-09-06 | 黑龙江省红光农场乳品厂 | Milk powder simulating breast milk for infants |
| CN1108478A (en) * | 1994-03-16 | 1995-09-20 | 黑龙江省林甸乳制品厂 | Selenium-intensified milk powder |
| CN1128103A (en) * | 1995-07-31 | 1996-08-07 | 浙江乳鸽宝保健品总公司 | Powdered milk containing active bifidobacterium and its producing method |
| CN1149400A (en) * | 1995-10-30 | 1997-05-14 | 莱阳科技城集团公司 | Naohuangjin instant milk powder and its preparation method |
| CN1300549A (en) * | 1999-12-17 | 2001-06-27 | 黑龙江乳业集团总公司 | Formula and process for preparing milk powder containing iron and protein for baby |
| CN1317254A (en) * | 2001-02-22 | 2001-10-17 | 李晓林 | Directly eatable milk powder and its preparing process |
-
2003
- 2003-11-03 CN CNB2003101032336A patent/CN1305383C/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1108478A (en) * | 1994-03-16 | 1995-09-20 | 黑龙江省林甸乳制品厂 | Selenium-intensified milk powder |
| CN1107656A (en) * | 1994-04-05 | 1995-09-06 | 黑龙江省红光农场乳品厂 | Milk powder simulating breast milk for infants |
| CN1128103A (en) * | 1995-07-31 | 1996-08-07 | 浙江乳鸽宝保健品总公司 | Powdered milk containing active bifidobacterium and its producing method |
| CN1149400A (en) * | 1995-10-30 | 1997-05-14 | 莱阳科技城集团公司 | Naohuangjin instant milk powder and its preparation method |
| CN1300549A (en) * | 1999-12-17 | 2001-06-27 | 黑龙江乳业集团总公司 | Formula and process for preparing milk powder containing iron and protein for baby |
| CN1317254A (en) * | 2001-02-22 | 2001-10-17 | 李晓林 | Directly eatable milk powder and its preparing process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1602708A (en) | 2005-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105146614B (en) | A kind of functional calcium fruit ferment, enzyme beverage and its production method | |
| CN102586148B (en) | Plant lactobacillus capable of relieving lead toxicity and application thereof | |
| JP5273695B2 (en) | Allergy prevention and / or treatment agent containing bifidobacteria as an active ingredient | |
| CN100484964C (en) | New use of tremella heteropolysaccharide or its extract | |
| CN110179121A (en) | It is a kind of to improve the 5-linked probiotic composition of enteron aisle, compound solid beverage and its preparation method and application | |
| CN104542977B (en) | A kind of health beverages and preparation method containing yam extract and bifidobacterium bifidum | |
| CN101171019A (en) | Immune function modulating agents | |
| CN106472704A (en) | Bean milk fermenting and producing probiotic bacteria functional drinkss and food and preparation method thereof | |
| CN1911118A (en) | Kefir mushroom freeze-dried powder, prodn. method and use thereof | |
| TW200804591A (en) | Lactic acid bacterium having immunoregulatory activity derived from moromi for wine fermentation | |
| CN100482104C (en) | Process for preparing lactic acid bacteria oral liquor | |
| WO2017069163A1 (en) | Infection preventive agent for infants | |
| CN1305383C (en) | Active bifidobacteria contained milk powder and its preparation method | |
| WO2017069167A1 (en) | Antiallergic agent for infants | |
| CN108795823B (en) | It is a kind of improve women pregnant and lying-in women's intestinal flora probiotics cultural method and application | |
| CN1306021C (en) | Method for preparing bifidobacterium lyophilized powder and its products and use | |
| CN103651804A (en) | Prebiotic and probiotic added milk tablet based on homology of medicine and food | |
| CN114921383B (en) | Probiotic preparation with cholesterol removal function and preparation method thereof | |
| CN101574375B (en) | Lactobacillus health-care capsule and preparation method thereof | |
| CN117460428A (en) | GOS preconditioned lactobacillus reuteri and GOS in final formulation | |
| CN117177673A (en) | Preconditioning of Lactobacillus reuteri | |
| CN110747140B (en) | Lactobacillus plantarum with selenium adsorption capacity and application thereof | |
| CN108464509B (en) | Application of novel lactobacillus fermentum in food field | |
| JP2022157465A (en) | Defecation promoter for infants | |
| CN112704234A (en) | Nutritional composition containing composite peptide and probiotics and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070321 Termination date: 20101103 |