CN101449830B - Mid-aged milk powder added with biostime and preparation method thereof - Google Patents
Mid-aged milk powder added with biostime and preparation method thereof Download PDFInfo
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- CN101449830B CN101449830B CN2008102498073A CN200810249807A CN101449830B CN 101449830 B CN101449830 B CN 101449830B CN 2008102498073 A CN2008102498073 A CN 2008102498073A CN 200810249807 A CN200810249807 A CN 200810249807A CN 101449830 B CN101449830 B CN 101449830B
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses middle age and elder milk powder added with synbiotics, which contains 35-65g of fresh milk (converted to dry matter), 12-32g of desalted whey powder, 2-6g of granulated sugar, 3-8g of vegetable oil, 0.4-2g of composite Prebiotics and 1.0*105-1*1011 of composite probiotics. The milk powder of the present invention can increase quantity of beneficial bacteria for middle age and elder, thereby promoting establishment of beneficial bacteria advantage. By adding probiotics, quantity of beneficial bacteria is increased in short time; by adding Prebiotics having selectivity increment effect to probiotics, added probiotics can fast colonize and advantage of beneficial bacteria in intestinal tract flora is maintained. The milk powder of the present invention can increase absorptivity and retention rate of calcium, is added with composite vitamin, composite mineral matter and other nutrients, and can provide comprehensive nutrition for middle age and elder.
Description
Technical field
The present invention relates to a kind of add symphysis unit be suitable for milk powder of the elderly and preparation method thereof, belong to technical field of processing dairy products.
Background technology
Generally speaking, since 50 years old, the phenomenon of physiological aging will little by little appear in the people, and the secreting function of various bodies of gland descends, and resistivity reduces, and is ill easily.In order to slow down the appearance of this situation, need to give with some functional foods of mid-aged population or in food and strengthen some functional nutrient compositions, more common now nutriment is person in middle and old age's milk powder, and milk powder is carried out the reinforcement of nutrition.
According to research, Bifidobacterium and the Bifidobacterium quantity difference in weak old man's enteron aisle in the long lived elder enteron aisle are huge, and the former is near young man, and the latter then sharply descends, even can not detect.Whether healthy the number of Bifidobacterium is relevant with aging degree, also be human body important symbol.
According to the interaction property of gut flora, can enteric bacteria be divided into beneficial bacterium, harmful bacterium and other bacterium three major types to body.Probio has the gut flora of improvement structure, suppresses pathogen, eliminates carcinogen, improves the immunity of organism factor, reduces physiological functions such as cholesterol.Usually; Flora in the enteron aisle is to maintain certain balance; Yet situation such as antibiotic, diarrhoea constipation are drunk, are used in disadvantaged group (the elderly, infant), irregular, the frequent smoking of living and diet, and the quantity of probio is destroyed; Thereby need take measures, recover the superiority of beneficial bacterium.
Prebiotics is meant can be by the food component of digestive juice digestion, and it can get into large intestine, is absorbed by beneficial bacterium specific in the large intestine, promotes the growth of beneficial bacterium, thereby promotes human beings'health.Present prebiotics typically refers to compound sugar, mainly contains soyabean oligosaccharides, FOS, galactooligosaccharide, xylo-oligosaccharide, inulin etc.
Symphysis unit becomes Synbiotics again, is meant the combination preparation of probio and prebiotics, both can bring into play the physiological bacterial activity of probio, optionally increases the quantity of probio again fast, and the effect that makes probio is significantly with lasting.
After the people gets into the middle age, can be easy to feel symptoms such as in poor health, osteoporosis, this is because arrived person in middle and old age's stage; Body organ function is tending towards degenerating; Absorbability, immunity all can descend, and cause bone calcium to lose easily, thereby cause illnesss such as osteoporosis, nutritional deficiency.A kind of effective method is to replenish the calcium; The present product of replenishing the calcium is a lot, we can say through the food or the product of replenishing the calcium, and the elderly can obtain enough calcium sources and replenish; But; Because the elderly's absorbability descends, and when replenishing the calcium, has also caused the loss and the waste in a large amount of calcium source, has increased the cost of replenishing the calcium virtually.The present inventor finds through test; The absorption and the reservation of calcium can improve in symphysis unit; Its main action principle possibly form the superiority of beneficial bacterium in the enteron aisle for symphysis unit; The SCFA that the probio secretion is a large amount of makes to be sour environment in the enteron aisle, thus the absorption and the utilization that improve calcium.
No matter in sum, a kind of milk powder that adds symphysis unit is provided, be to recovering, keep the superiority of beneficial bacterium in the enteron aisle, still to the absorption and the reservation of calcium, all having important function and significance.In the prior art, add prebiotics or probio in the dairy produce bibliographical information though have, its effect is general, can not recover and keep the superiority of profitable strain in the enteron aisle effectively.
Summary of the invention
To the deficiency of above-mentioned prior art, the invention provides a kind of milk powder that is applicable to the elderly's interpolation symphysis unit, the present invention also provides its preparation method.
A kind of person in middle and old age's milk powder that adds symphysis unit comprises following component: fresh milk (being converted into dry), 35～65 grams; Desalted whey powder, 12～32 grams; Granulated sugar, 2～6 grams; Vegetable oil, 3～8 grams; Complex prebiotics, 0.4～20 gram; Compound probiotic, 1.0 * 10
5～1.0 * 10
14Cfu.
Said fresh milk can be milk, goat milk; The form of fresh milk can be a liquid milk, also can be to transport for ease and the milk powder that directly dusted and obtained by liquid milk.The preferred peanut oil of said vegetable oil, olive oil, corn wet goods are rich in the vegetable oil of unrighted acid.
The first person in middle and old age's milk powder of said interpolation symphysis also comprises following component: B B-complex, 0.035 gram; Composite mineral matter, 0.6 gram; Nucleotides, 0.12 gram; Taurine, 0.04 gram; DHA (DHA), 0.35 gram; Arachidonic acid (ARA), 0.3 gram; Lecithin, 0.5 gram.
The content of said complex prebiotics is 2～10 grams.
Said complex prebiotics is a kind of in oligoisomaltose, FOS, xylo-oligosaccharide, galactooligosaccharide, chitosan oligomer, soyabean oligosaccharides, inulin, the milk ketose or appoints several kinds arbitrary proportion combination; The preferred combination of compound sugar more than three kinds.
Said compound probiotic is a kind of in bifidobacterium strains, lactobacillus strains, streptococcus bacterial strain, lactococcus bacterial strain, the Bacillus strain or appoints several kinds arbitrary proportion combination.The combination of preferred bifidobacterium strains and lactobacillus strains.
Said bifidobacterium strains is bifidobacterium infantis, bifidobacterium adolescentis, bifidobacterium longum or bifidobacterium breve; Said lactobacillus strains is lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus thermophilus or Lactobacillus casei.
Said B B-complex is composed of the following components: vitamin A, 6 grams; Vitamin D, 4 grams; Vitamin E, 0.1 gram; Vitamin K
1, 0.035 gram; Cobastab
1, 1.6 grams; Cobastab
2, 0.8 gram; Cobastab
6, 0.38 gram; Cobastab
12, 0.015 gram; Vitamin C, 4 grams; Niacinamide usp, 8 grams; Folic acid, 0.08 gram; Calcium pantothenate, 6 grams; Biotin, 0.04 gram.
Said composite mineral matter is composed of the following components: ferrous lactate, 60 grams; Zinc lactate, 10 grams; Copper sulphate, 2.5 grams; Potassiumiodate, 0.13 gram; Calcium carbonate, 435.2 grams; Manganese sulfate, 0.18 gram.
A kind of preparation method who adds person in middle and old age's milk powder of symphysis unit, step is following: mix other components except that compound probiotic, B B-complex, get mixed solution; Use high pressure homogenizer under 10～20MPa, to carry out homogeneous then, carry out spray-drying or freeze drying then, get powder product; Add compound probiotic, complex microorganism then; Mixing, vacuum packaging had both got milk powder.
Milk powder of the present invention also can adopt other any suitable methods to process.
Milk powder of the present invention can be used for the increase of middle-aged and old beneficial bacterium quantity, thereby promotes the foundation of beneficial bacterium advantage.Through adding probio, increase that can short time internal stimulus beneficial bacterium quantity, and, can make the quick field planting of probio of interpolation through adding prebiotics to the selective value-added effect of probio, keep the advantage of beneficial bacterium at gut flora.Milk powder of the present invention can also improve the absorptivity and the retention rate of calcium.
At present, life is usually usually killed the pathogenic bacteria in the body through taking antibiosis after being ill, still; Because antibiotic does not have identity; Can kill harmful bacterium in the enteron aisle and one of beneficial bacterium, make the rapid minimizing of quantity of the flora in the enteron aisle and in the mid-aged population of administration of antibiotics, take symphysis unit, then can recover the balance of flora in the enteron aisle; And beneficial bacterium had more significantly value-added effect, can strengthen beneficial bacterium superiority in the flora in enteron aisle.
The symphysis unit that adds among the present invention is the best of breed that draws through behind the verification experimental verification repeatedly for the organic assembling of compound probiotic and complex prebiotics, and its effect that plays not is the simple superposition of each single material, but organically combines.Also added B B-complex, composite mineral matter and other nutriment in the milk powder of the present invention, can comprehensive nutrition be provided to mid-aged population.Added the vegetable oil that peanut oil, olive oil, corn wet goods are rich in unrighted acid, can increase the content of unrighted acid and essential fatty acids linoleic in the product, the health of improving mid-aged population has been had good effect.Added essential fatty acids such as DHA, ARA, also had nutriments such as lecithin, nucleotides, be beneficial to the growth of mid-aged population brain cell, very big benefit has been arranged keeping mid-aged population memory.
The specific embodiment
Below in conjunction with embodiment and Test Example the present invention is further described:
Embodiment 1: prepare a kind of person in middle and old age's milk powder that adds symphysis unit, wherein, the prescription of milk powder is following:
Milk (material of giving money as a gift) 55g
Desalted whey powder 22g
Granulated sugar 3g
Peanut oil 6g
Complex prebiotics 15g
Compound probiotic 1.0 * 10
8Cfu
B B-complex 0.035g
Composite mineral matter 0.6g
Nucleotides 0.12g
Taurine 0.04g
DHA 0.35g
ARA 0.3g
Lecithin 0.5g.
Wherein, the composition weight proportioning of complex prebiotics is: soyabean oligosaccharides: FOS: xylo-oligosaccharide: gossypose=10: 5: 2: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium longum: bifidobacterium adolescentis: lactobacillus acidophilus: streptococcus thermophilus=3: 2: 2: 1.
The prescription of B B-complex is: vitamin A, 6g; Vitamin D, 4g; Vitamin E, 0.1g; Vitamin K
1, 0.035g; Cobastab
1, 1.6g; Cobastab
2, 0.8g; Cobastab
6, 0.38g; Cobastab
12, 0.015g; Vitamin C, 4g; Niacinamide usp, 8g; Folic acid, 0.08g; Calcium pantothenate, 6g; Biotin, 0.04g.
The prescription of composite mineral matter is: ferrous lactate, 60g; Zinc lactate, 10g; Copper sulphate, 2.5g; Potassiumiodate, 0.13g; Calcium carbonate, 435.2g; Manganese sulfate, 0.18g.
Preparation technology is: mix other components except that compound probiotic, B B-complex, get mixed solution, use high pressure homogenizer under 10～20MPa, to carry out homogeneous then; Carry out spray-drying then, get powder product, add compound probiotic, complex microorganism then; Mixing, vacuum packaging.
Embodiment 2: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is following:
Milk (material of giving money as a gift) 62g
Desalted whey powder 16g
Granulated sugar 2g
Olive oil 4g
Complex prebiotics 5g
Compound probiotic 1.0 * 10
14Cfu
B B-complex 0.035g
Composite mineral matter 0.6g
Nucleotides 0.12g
Taurine 0.04g
DHA 0.35g
ARA 0.3g
Lecithin 0.5g.
Wherein, the composition weight proportioning of complex prebiotics is: soyabean oligosaccharides: galactooligosaccharide: xylo-oligosaccharide: gossypose=10: 8: 2: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium breve: bifidobacterium adolescentis: lactobacillus acidophilus=3: 4: 2.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 3: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is following:
Milk (material of giving money as a gift) 42g
Desalted whey powder 30g
Granulated sugar 4g
Corn oil 8g
Complex prebiotics 8g
Compound probiotic 1.0 * 10
11Cfu
B B-complex 0.035g
Composite mineral matter 0.6g
Nucleotides 0.12g
Taurine 0.04g
DHA 0.35g
ARA 0.3g
Lecithin 0.5g.
Wherein, the composition weight proportioning of complex prebiotics is: milk ketose: FOS: xylo-oligosaccharide: gossypose=5: 5: 4: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium adolescentis: lactobacillus acidophilus: streptococcus thermophilus=5: 2: 2.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 4: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is following:
Milk (material of giving money as a gift) 35g
Desalted whey powder 12g
Granulated sugar 2g
Peanut oil 3g
Complex prebiotics 0.4g
Compound probiotic 1.0 * 10
5Cfu
B B-complex 0.035g
Composite mineral matter 0.6g
Nucleotides 0.12g
Taurine 0.04g
DHA 0.35g
ARA 0.3g
Lecithin 0.5g.
Wherein, the composition weight proportioning of complex prebiotics is: oligoisomaltose: FOS: xylo-oligosaccharide: galactooligosaccharide=4: 10: 8: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium infantis: bifidobacterium adolescentis: lactobacillus bulgaricus=2: 2: 2.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 5: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is following:
Milk (material of giving money as a gift) 65g
Desalted whey powder 32g
Granulated sugar 6g
Olive oil 8g
Complex prebiotics 20g
Compound probiotic 1.0 * 10
11Cfu
B B-complex 0.035g
Composite mineral matter 0.6g
Nucleotides 0.12g
Taurine 0.04g
DHA 0.35g
ARA 0.3g
Lecithin 0.5g.
Wherein, the composition weight proportioning of complex prebiotics is: chitosan oligomer: soyabean oligosaccharides: inulin: milk ketose=2: 4: 2: 10.
The composition weight proportioning of compound probiotic is: bifidobacterium infantis: bifidobacterium adolescentis: Lactobacillus casei=1: 4: 3.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 6: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is following:
Goat milk (material of giving money as a gift) 50g
Desalted whey powder 22g
Granulated sugar 4g
Corn oil 5g
Complex prebiotics 10g
Compound probiotic 1.0 * 10
9Cfu
B B-complex 0.035g
Composite mineral matter 0.6g
Nucleotides 0.12g
Taurine 0.04g
DHA 0.35g
ARA 0.3g
Lecithin 0.5g.
Wherein, the composition weight proportioning of complex prebiotics is: oligoisomaltose: xylo-oligosaccharide: inulin: chitosan oligomer=2: 2: 8: 10.
The composition weight proportioning of compound probiotic is: bifidobacterium longum: bifidobacterium breve: lactobacillus acidophilus=6: 1: 4.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 7: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is following:
Milk (material of giving money as a gift) 60g
Desalted whey powder 8g
Granulated sugar 5g
Peanut oil 4g
Complex prebiotics 2g
Compound probiotic 1.0 * 10
10Cfu
B B-complex 0.035g
Composite mineral matter 0.6g
Nucleotides 0.12g
Taurine 0.04g
DHA 0.35g
ARA 0.3g
Lecithin 0.5g.
Wherein, the composition weight proportioning of complex prebiotics is: FOS: soyabean oligosaccharides: xylo-oligosaccharide: milk ketose=5: 2: 8: 10.
The composition weight proportioning of compound probiotic is: bifidobacterium infantis: bifidobacterium longum: lactobacillus thermophilus=1: 8: 4.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Test Example 1: following animal experiment has been described the comparison that complex prebiotics among the present invention improves the result to improvement and the single prebiotics of rat gut flora.
1 materials and methods:
1.1 animal used as test: 64 of SPF level SD male rats (Jinan Ge Run zoopery scientific and technological development Co., Ltd provides), body weight 100 ± 10g is divided into 8 groups at random, 8 every group.
1.2 divide into groups:
Group 1: control group: the basal feed of feeding.
Group 2: single prebiotics: basal feed adds 4% oligoisomaltose.
Group 3: single prebiotics: basal feed adds 4% galactooligosaccharide.
Group 4: single prebiotics: basal feed adds 4% soyabean oligosaccharides.
Group 5: complex prebiotics: (weight proportion of each component of prebiotics is the complex prebiotics of basal feed interpolation 4%: xylo-oligosaccharide 2+ galactooligosaccharide 10+ inulin 15).
Group 6: complex prebiotics: (weight proportion of each component of prebiotics is the complex prebiotics of basal feed interpolation 4%: oligoisomaltose 15+ xylo-oligosaccharide 2+ gossypose 5).
Group 7: complex prebiotics: (weight proportion of each component of prebiotics is the complex prebiotics of basal feed interpolation 4%: soyabean oligosaccharides 10+ FOS 5+ xylo-oligosaccharide 2+ inulin 15).
Group 8: complex prebiotics: (weight proportion of each component of prebiotics is the complex prebiotics of basal feed interpolation 4%: oligoisomaltose 15+ gossypose 5+ milk ketose 10+ inulin 15).
Each group is raised free diet separately.The feed of feeding all is SPF level feeds, drinks water to be pure water.
1.3 sample collection: feeding time respectively was 3 weeks; Employings in the 0th day, 3 days, 7 days, 10 days, 14 days, 21 days respectively at after the experiment beginning force method get the rat fresh excreta; Be loaded on and weigh and be equipped with in the sterile test tube of physiological saline and bead; Test tube after the dress appearance is weighed once more, tries to achieve sample weight.Build to be placed on the liquid quick oscillation device behind the pipe close and mix,, become series with physiological saline with 10 times ratio doubling dilution then to make even suspension.
1.4 medium preparation: adopt the aerobic culture medium EMB of selectivity, EC, selectivity anaerobic culture medium KV, TPY and MRS are according to the requirement preparation of " Clinical Anaerobic Bacteria inspection manual ".
1.5 inoculated and cultured: the sample series dilution 0.01mL that gets above-mentioned processing is inoculated on the culture medium, places anaerobism and aerobic environment to cultivate respectively.37 ℃ of aerobic cultivations, 24h observed result, observed result behind the anaerobism 48h.The record clump count is also made identification of strains.
1.6 calculate and data statistics: the result representes with logarithm value, computing formula: the viable bacteria colony in every gram ight soil is counted logarithm value=log{ [sample weight (g)+dilution liquid measure (mL)]/sample weight * dilution factor * bacterium colony number * 100}.All data are all analyzed with the SPSS statistical software.
2 results:
Adopt the aerobic culture medium EMB of selectivity, EC respectively, selectivity anaerobic culture medium KV, TPY and MRS detect and respectively organize flora in the rat stool sample 5: enterobacteria, enterococcus, bacteroid, Bifidobacterium and lactobacillus, testing result are seen table 1-5.
Table 1 is respectively organized rat stool sample enterobacteria crowd testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1, c1, d1, e1 represent to have compared significant difference between two groups, P＜0.05; A2, b2, c2 represent to have compared between two groups utmost point significant difference, P＜0.01.
Table 2 is respectively organized rat stool sample enterococcus crowd testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1, c1, d1, e1 represent to have compared significant difference between two groups, P＜0.05; A2, b2, c2 represent to have compared between two groups utmost point significant difference, P＜0.01.
Table 3 is respectively organized rat stool sample bacteroid crowd testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1 represent to have compared significant difference between two groups, P＜0.05.
Table 4 is respectively organized rat stool sample Bifidobacteria population testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1, c1, d1, e1, f1, g1, h1 represent to have compared significant difference between two groups, P＜0.05; A2, b2, c2 represent to have compared between two groups utmost point significant difference, P＜0.01.
Table 5 is respectively organized rat stool sample lactobacillus crowd testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1 represent to have compared significant difference between two groups, P＜0.05; A2 representes to have compared between two groups utmost point significant difference, P＜0.01.
Table 1, table 2 data all show, single prebiotics makes enterobacteria, enterococcus that the trend of minimizing arranged, and that complex prebiotics reduces is more; Though table 3 shows the bacteroid of adding complex prebiotics the trend of minimizing is arranged, variation tendency is not obvious.Table 4, table 5 data show that compare with single prebiotics, complex prebiotics can make beneficial bacterium that good growth trend is arranged, but relative Bifidobacterium, the increase trend of lactobacillus is not clearly.
Can find out by above-mentioned test; Different prebioticses has very big difference to the influence of rat gut flora; And the complex prebiotics of various combination never is simple overlaying relation to the influence of rat gut flora; Therefore, to add the complex prebiotics in the milk powder to be the complex prebiotics combination with optimum efficiency that obtains through test in the present invention.
Test Example 2: following animal experiment described symphysis unit among the present invention to administration of antibiotics after rat recover the beneficial effect of beneficial bacterium advantage in the gut flora.
1 materials and methods:
1.1 animal used as test: 24 of SPF level SD male rats (Jinan Ge Run zoopery scientific and technological development Co., Ltd provides), body weight 100 ± 10g is divided into 3 groups at random, 8 every group.
1.2 divide into groups:
Group 1: control group: the basal feed of feeding.
Group 2: experiment contrast group: the basal feed of feeding, 0 day administration of antibiotics (Lincomycin Hydrochloride, 200mg/Kg/ day, per os was irritated stomach) once.
Group 3: experimental group: the basal feed of feeding, and administration of antibiotics, after 3 days, feeding, (weight proportion of each component of symphysis unit is the feed that adds 4% symphysis unit: xylo-oligosaccharide 2+ galactooligosaccharide 10+ soyabean oligosaccharides 10+ gossypose 5+ bifidobacterium infantis 10
3Cfu/g+ bifidobacterium adolescentis 10
2Cfu/g+ lactobacillus acidophilus 10
2Cfu/g).
Each group is raised free diet separately.The feed of feeding all is SPF level feeds, drinks water to be pure water.
1.3 sample collection: feeding time was 3 weeks; Employing in the 0th, 3,7,14,21 day respectively at after the experiment beginning forces method get the rat fresh excreta; Be loaded on and weigh and be equipped with in the sterile test tube of physiological saline and bead, the test tube after the dress appearance is weighed once more, tries to achieve sample weight.Build the pipe post-games and be placed on the liquid quick oscillation device and mix,, become serial with physiological saline with 10 times ratio doubling dilution then to make even suspension.
1.4 medium preparation: adopt the aerobic culture medium EMB of selectivity, EC, selectivity anaerobic culture medium KV, TPY and MRS are according to the requirement preparation of " Clinical Anaerobic Bacteria inspection manual ".
1.5 inoculated and cultured: the sample series dilution 0.01ml that gets above-mentioned processing is inoculated on the culture medium, places anaerobism and aerobic environment to cultivate respectively.37 ℃ of aerobic cultivations, 24h observed result, observed result behind the anaerobism 48h.The record clump count is also made identification of strains.
1.6 calculate and analyze: the result representes with various bacterium proportions
Computing formula:
Viable bacteria colony number in every gram ight soil=[sample weight (g)+dilution liquid measure (ml)]/sample weight * dilution factor * bacterium colony number
2 results:
Adopt the aerobic culture medium EMB of selectivity, EC respectively, selectivity anaerobic culture medium KV, TPY and MRS detect and respectively organize flora in the rat stool sample 5: enterobacteria, enterococcus, bacteroid, Bifidobacterium and lactobacillus, and calculate, testing result is seen table 6.
Table 6 symphysis unit is to the experimental result that influences of big gut flora
Can find out from experimental data, the recovery of the rat gut flora of symphysis unit after and beneficial bacterium advantage for administration of antibiotics establish good benefit.
Test Example 3: following animal experiment has been described symphysis unit among the present invention to the absorptivity that improves calcium and the beneficial effect of retention rate.
1 materials and methods:
1.1 animal used as test: 32 of SPF level SD female rats (Jinan Ge Run zoopery scientific and technological development Co., Ltd provides), 60-70g is divided into 4 groups immediately, 8 every group.
1.2 divide into groups:
Group 1: low calcium control group: the low calcium feed of feeding.
Group 2: normal control group: the basal feed of feeding.
Group 3: experiment contrast group: feed and hang down the calcium feed and in feed, add 4% symphysis unit;
Wherein, the weight proportion of each component of symphysis unit is: soyabean oligosaccharides 10+ FOS 5+ xylo-oligosaccharide 2+ gossypose 5+ probio 10
6Cfu/g milk powder (bifidobacterium longum: bifidobacterium adolescentis: lactobacillus acidophilus: streptococcus thermophilus=3: 2: 2: 1).
Group 4: experimental group: the basal feed and in feed, add 4% symphysis unit of feeding;
Wherein, the weight proportion of each component of symphysis unit is: soyabean oligosaccharides 10+ FOS 5+ xylo-oligosaccharide 2+ gossypose 5+ probio 10
6Cfu/g milk powder (bifidobacterium longum: bifidobacterium adolescentis: lactobacillus acidophilus: streptococcus thermophilus=3: 2: 2: 1).
Each group is raised free diet separately.The feed of feeding all is SPF level feeds, drinks water to be that pure water, feeding time were 3 weeks.
1.3 detection index: respectively at carrying out metabolic test in the 20th day, the 21st day.Rat is placed in the metabolic cage two days, collects rat ight soil and urine, and write down two days stool weight and amount of urine.Off-test is gathered rat blood and is measured blood calcium, puts to death rat then, separates the left side femur, detects bone calcium.
The mensuration of calcium content: feed and ight soil are dried in baking oven through evenly mixing and crossing 20 mesh sieves, put in the drier and weigh after the cooling, and is levigate.Note preventing the pollution in the sample preparation process.Getting the rats with left femur dries to constant weight in 105 ℃ of baking ovens.The assay of rat femur calcium, excrement calcium, urine calcium, blood calcium and feed calcium (low calcium feed and basal feed) all adopts atomic absorption spectrophotometer.
1.4 calculate:
Take in calcium content (mg/g) * 2 day feed consumption in calcium (mg)=feed
The absorptivity %=of calcium (taking in calcium one excrement calcium)/absorption calcium * 100%
Femur calcium retention rate (%)=femur calcium/absorption calcium * 100%
2 results:
Result of the test is analyzed, and calculated the absorptivity and the retention rate of calcium, the result sees table 7.
Table 7 symphysis unit is to the experimental result that influences of the absorptivity of calcium and retention rate
Can find out from result of the test, in basal feed, add absorptivity and the retention rate that calcium can obviously improve in symphysis unit.
In sum, can expect that the first milk powder of interpolation symphysis of the present invention not only can promote the foundation of beneficial bacterium advantage in the enteron aisle, can also improve the absorptivity and the retention rate of calcium, be very suitable for the elderly and eat.
Claims (9)
1. the person in middle and old age's milk powder that adds symphysis unit is characterized in that, comprises following component: the fresh milk material of giving money as a gift, 35～65 grams; Desalted whey powder, 12～32 grams; Granulated sugar, 2～6 grams; Vegetable oil, 3～8 grams; Complex prebiotics, 0.4～20 gram; Compound probiotic, 1.0 * 10
5～1.0 * 10
14Cfu;
Said complex prebiotics is one of following combination:
1. 10 parts+inulin of 2 parts+galactooligosaccharide of xylo-oligosaccharide is 15 parts;
2. 2 parts+gossypose of 15 parts+xylo-oligosaccharide of oligoisomaltose is 5 parts;
3. 2 parts+inulin of 5 parts+xylo-oligosaccharide of 10 parts+FOS of soyabean oligosaccharides is 15 parts;
4. 10 parts+inulin of 5 parts+milk ketose of 15 parts+gossypose of oligoisomaltose is 15 parts;
Said umber is a weight portion.
2. a kind of person in middle and old age's milk powder that adds symphysis unit according to claim 1 is characterized in that, also comprises following component: B B-complex, 0.035 gram; Composite mineral matter, 0.6 gram; Nucleotides, 0.12 gram; Taurine, 0.04 gram; DHA, 0.35 gram; Arachidonic acid, 0.3 gram; Lecithin, 0.5 gram.
3. a kind of person in middle and old age's milk powder that adds symphysis unit according to claim 1 is characterized in that: the content of said complex prebiotics is 2～10 grams.
4. a kind of person in middle and old age's milk powder that adds symphysis unit according to claim 1 is characterized in that: said compound probiotic is to appoint several kinds arbitrary proportion to make up in bifidobacterium strains, lactobacillus strains, streptococcus bacterial strain, lactococcus bacterial strain, the Bacillus strain.
5. a kind of person in middle and old age's milk powder that adds symphysis unit according to claim 4, it is characterized in that: said compound probiotic is the combination of bifidobacterium strains and lactobacillus strains.
6. a kind of person in middle and old age's milk powder that adds symphysis unit according to claim 5, it is characterized in that: said bifidobacterium strains is bifidobacterium infantis or bifidobacterium adolescentis or bifidobacterium longum or bifidobacterium breve; Said lactobacillus strains is lactobacillus bulgaricus or lactobacillus acidophilus or lactobacillus thermophilus or Lactobacillus casei.
7. a kind of person in middle and old age's milk powder that adds symphysis unit according to claim 2, it is characterized in that: said B B-complex is composed of the following components: vitamin A, 6 grams; Vitamin D, 4 grams; Vitamin E, 0.1 gram; Vitamin K
1, 0.035 gram; Cobastab
1, 1.6 grams; Cobastab
2, 0.8 gram; Cobastab
6, 0.38 gram; Cobastab
12, 0.015 gram; Vitamin C, 4 grams; Niacinamide usp, 8 grams; Folic acid, 0.08 gram; Calcium pantothenate, 6 grams; Biotin, 0.04 gram.
8. a kind of person in middle and old age's milk powder that adds symphysis unit according to claim 2, it is characterized in that: said composite mineral matter is composed of the following components: ferrous lactate, 60 grams; Zinc lactate, 10 grams; Copper sulphate, 2.5 grams; Potassiumiodate, 0.13 gram; Calcium carbonate, 435.2 grams; Manganese sulfate, 0.18 gram.
9. the described a kind of preparation method who adds person in middle and old age's milk powder of symphysis unit of claim 2 is characterized in that step is: mix other components except that compound probiotic, B B-complex; Get mixed solution, use high pressure homogenizer under 10～20MPa, to carry out homogeneous then, carry out spray-drying or freeze drying then; Get powder product; Add compound probiotic, B B-complex then, mixing, vacuum packaging.
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| CN110115295A (en) * | 2019-04-23 | 2019-08-13 | 浙江科技学院 | One kind being rich in DHA, AA and ALA Quinquagenarian formula milk powder preparation method |
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