CN108795823B - It is a kind of improve women pregnant and lying-in women's intestinal flora probiotics cultural method and application - Google Patents
It is a kind of improve women pregnant and lying-in women's intestinal flora probiotics cultural method and application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a kind of cultural method for improving women pregnant and lying-in women's intestinal flora probiotics and applications.Porous material is added in the culture medium containing the somatomedin for promoting growth of probiotics breeding, and the volume ratio of culture medium and porous material is 1:0.001-1:1000, then is inoculated with probiotics in the medium and is cultivated, and realizes probiotics High Density Cultivation;Probiotics obtained can be used for preparing the products such as food, food additives, medicine, feed or environmental protection treatment.The present invention, which obtains high density probiotics, has the performances such as good heat resistance, acid resistance and drug resistance, has broad application prospects in fields such as food, medicine, feed, environmental protection.
Description
Technical field
The present invention relates to a kind of cultural method of probiotics for improving women pregnant and lying-in women's intestinal flora and applications, belong to micro- life
Object preparation culture technique field.
Background technique
Probiotics, English mono- word of probiotics are translated into " for life " from Greek " for live " derivation, are
Viable bacteria containing physiological activity after oral or other administration modes take in quantity appropriate, can be colonized in when by body
Host simultaneously improves host's microecological balance, to play beneficial effect.Recent studies indicate that the intracorporal bacterium of people is up to
Bacterium in more than 1000 kinds, especially gastrointestinal tract is the abundantest, and Zhan Quanshen micro-ecological bacterial sum 80% or so, is human body cell
10 times, it is known as " the second genome " of the mankind.These bacteriums facilitate human consumption's food, generate vitamin to prevent food
The disease that middle bacterium is induced, while stimulating immune system.With going deep into for research, find enteric microorganism in many chronic diseases
In disease and symptom, such as inflammation, obesity, diabetes, also cancer also play key effect.The quantity and gene dosage of microorganism
It is surprising, the strong health for influencing the mankind.These are found to be probiotic products in food, health care product, medicine, cultivation, environment, health
The application in the fields such as articles brings huge motive force.Currently, probiotics is widely used in Yoghourt, sour milk beverage, prebiotic
In the products such as bacteria powder, animal feed, environmental improvement, relatively complete and mature probiotics industry is formd.
It is worth noting that, probiotics could generate in host when probiotics only reaches certain quantity levels
Corresponding effect.So needing to guarantee contained prebiotic bacterium number living by the food of functional component, health care product or drug of probiotics
Amount is maintained at certain level.This demand proposes corresponding requirement to the production and preparation of probiotics.In the training of probiotics
During supporting, realizes High Density Cultivation, not only can satisfy the functional requirement of subsequent probiotic products, probiotics can also be improved
Production efficiency in production link reduces volume of culture, shortens the production cycle, reduces production cost.Therefore, probiotics is highly dense
Degree culture is the key technical problem in current probiotics exploitation and production.
In liquid fermentation system, Chinese invention patent (publication number: CN 101230323A) adds alkalinity during the cultivation process
Suffered stress material acid or oxygen, make finally to produce when salt and deoxidier are to eliminate lactobacillus acidophilus or bifidobacterium bifidum fermentation
Number of viable improves 2-4 times in product.Chinese invention patent (publication number: CN 101418270A) is in the training of Lactobacillus casei Zhang
During supporting, by the method for the addition neutralizers such as NaOH, KOH or ammonium hydroxide and logical nitrogen, 1011CFU/mL or more viable bacteria is obtained
The freeze-dried vaccine powder of several Lactobacillus caseis.Hayakawa K et al. (J Ferment Bioeng, 1990,70,404) uses film skill
Art and fermentor are coupled, and are constantly filtered to remove the stress ingredient such as lactic acid, are realized that highest cell density is up to 1011CFU/mL, the party
Method need to expend mass propgation base, and be only limitted to laboratory level, be difficult to be expanded to production application scale.
In semisolid or solid fermentation system, Chinese invention patent (publication number: CN 105132321A) using by wheat bran and
The culture medium of the material compositions such as dregs of beans, inoculation enterococcus faecium carry out solid state fermentation, and the viable bacteria content in dry products is up to 3.8
×1010CFU/g。
Similar also Chinese patent (publication number: CN 101555510B;CN 101555511B;CN 103421710B;
CN 102907563B;CN 103289937B;CN 1212391C) it is all made of dregs of beans, wheat bran, corncob, rice bran, peanut shell, shallow lake
The agricultural wastes such as powder, corn flour, turf or agricultural product solid medium as main component, microbe inoculation carry out highly dense
Degree ferments, and the viable count in product is essentially 1011CFU/g.This kind of product is mainly used for the metabolism such as animal feed or progress drug
Product production.
Probiotics is a kind of active microorganism beneficial to host, is to be colonized in human body intestinal canal, in reproductive system, can generate
Definite health efficacy so as to improve host's microecological balance, play the active beneficial microorganism general name of beneficial effect.Human body moves
Beneficial bacterium or fungi mainly have in object: clostridium butyricum, lactobacillus, Bifidobacterium, actinomyces, saccharomycete etc..Generation at present
The most powerful product of the function of being studied in boundary be mainly the above all kinds of microorganism groups at composite reactive probiotics, be widely applied
In bioengineering, industrial or agricultural, food safety and life and health field.
The benefit of probiotics is very more, such as prevents or improves diarrhea, alleviates lactase deficiency shape, prevention system genitale togetherness
It contaminates, enhance human immunity, enteron aisle digestive system health, reduction serum cholesterol and help is promoted to absorb nutritional ingredient.
Common probiotics such as Bifidobacterium is existing a kind of physiological bacterium in human body, it is close not with the health of human body
It can divide, if Bifidobacterium will generate lactic acid and acetic acid after sugar decomposition, make enteron aisle in acidity, result can control to be caused by harmful bacteria
Abnormal fermentation, and stimulate enterocinesia, play the role of releasing constipation, bifidobacterium preparations can also inhibit toxigenic
Harmful bacteria quantity, to play good therapeutic effect to liver patient, in addition it is solid can to influence gallbladder for the beneficial bacteriums such as Bifidobacterium
The metabolism of alcohol is translated into the non-absorbent steroids of human body, reduces the concentration of Blood Cholesterol, thus to hypertension and moves
Arteries and veins hardening has certain preventive and therapeutic effect.
There are also the sections that common lactobacillus is bacterium, are Gram-positive, sporeless bacterium, lactic acid bacteria is in nature
Be distributed in boundary it is very wide, plant surface, dairy products, meat products, beer, grape wine, fruit juice, brewer's wort, fermented dough, sewage with
And in human and animal excreta, separate.The food of Chinese tradition, such as pickles, hot pickled mustard tuber, pickle and the production of wine brewing preservation technology
This effect of lactic acid bacteria is exactly utilized, to enteron aisle and good absorbing.
The many health problems of human body are caused by internal flora is unbalance, and determinant is the balance of Tiny ecosystem, can be passed through
Internal flora is set to achieve the purpose that the ecological balance again to realize alleviation and treatment.For example diarrhea, constipation, vagina infection etc. are comprehensive
Symptom has already passed through years of researches and practice both at home and abroad, it was demonstrated that can be effectively using the probiotics specifically confirmed through clinic
It is treated.
These are found to be probiotic products in the application in the fields such as food, health care product, medicine, cultivation, environment, amenities
Bring huge motive force.Currently, probiotics be widely used in Yoghourt, sour milk beverage, probiotics powder agent, animal feed,
In the products such as environmental improvement, relatively complete and mature probiotics industry is formd.
If Chinese patent 201110442521.9 discloses a kind of food product, it is related to being preferentially used for the people's at paediatrics age
The administration form of load capacity height and the probiotic bacteria of stable load, the invention further relate to chocolate flavoured Probiotic supplement,
Especially include interior section and exterior section probiotic tablet, but the taste of the invention be it is chocolate flavoured, limit consumption
Person crowd, although the food of the invention is liked by children deeply, most of adult does not like the food of dietetic chocolate taste
Product, and the food is also not suitable for large amounts of food.
In addition, pregnancy and lactation are two special body periods of mother, if this stage is sick, it is easy to fall into nothing
Available awkward situation is wanted, at this time probiotic supplemented, the resistance of mother on the one hand can be improved, on the other hand can also improve mother's
Healthy malaise symptoms.In addition many pregnant and lying-in women can be easy to happen constipation, and probiotics can play good work during this time
With because probiotics plays the role of prevention and relief of constipation, probiotics refers to by adjusting intestinal flora, and it is strong to improve enteron aisle
The viable bacteria of health, probiotics are adjustable intestinal flora balance, maintain the active microorganism of intestinal health.Probiotics can not only prevent
Allergic constitution, but also the immunity of pregnant and lying-in women and baby can be improved, pregnant and lying-in women take probiotics in right amount and are conducive to health.
As Chinese patent 201110442521.9 discloses a kind of compound probiotic, its answering in treatment anaphylactia
With and allergy free probiotic electuary for pregnant, the compound probiotic of the invention its by bifidobacterium lactis Bi-07, Lactobacillus rhamnosus
LR22, lactobacillus acidophilus NCFM composition, can not only stimulate the secretion of IFN-γ and IL-2 in animal body, and can inhibit immune
The secretion of globulin E (IgE) and IL-4, to play prevention and treatment anaphylactia, and pharmacodynamic test shows the drug system
Agent has remarkable effect to IL-2 and IFN-γ is improved.In addition, the IgE content of test group is significantly reduced with control group ratio,
But the invention do not provide how the method for the culture of the high density and activity compound probiotic, therefore it is compound in the invention
Probiotics is likely to over time, and the quantity of beneficial bacterium is reduced rapidly, thus the value that loses one's health.
Therefore it provides a kind of probiotic composition that can improve pregnant and lying-in women's intestinal flora has very big realistic meaning.
So need at present it is a kind of improve women pregnant and lying-in women's intestinal flora probiotics cultural method and application;Need a kind of improvement female
The property multifarious probiotic composition of pregnant and lying-in women's intestinal flora.
Summary of the invention
In order to solve the problems, such as background technique, the purpose of the present invention is to provide a kind of improvement women pregnant and lying-in women's intestines
The cultural method of road flora probiotics and application, it is characterised in that include the following steps:
The technical solution adopted by the invention is as follows:
Porous material is added in the culture medium containing the somatomedin for promoting growth of probiotics breeding, culture medium and more
The volume ratio of Porous materials is 1:0.001-1:1000, then is inoculated with probiotics in the medium and is cultivated, and realizes that probiotics is high
Density culture.
The culture is Anaerobic culturel, and the probiotics being inoculated with is the microorganism of anaerobism or amphimicrobian.
Anaerobic culturel is to cultivate 12-48h at 30-42 DEG C.
After culture, the probiotics gathers the surface or inside or surface and inside of porous material, obtains
Total viable count is up to 1011-1015CFU/g or more in product after drying.Viable count is influenced by factors such as drying means, cold
It is higher that viable count in dry product is lyophilized.
Preparation-obtained probiotics has the performances such as good heat resistance, acid resistance and drug resistance.
The culture medium is liquid, semisolid or solid-state, and cultivating system can be Liquid Culture, Semi-solid cell culture and consolidate
Any one of three kinds of training methods of body culture, the porous material are solid-state.
The invention discloses a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora, the probiotic groups
It closes object and contains that resistant dextrin, inulin, oligofructose, bifidobacterium lactis, Lactobacillus rhamnosus and Lactobacillus casei etc. are more, this is prebiotic
Bacteria composition can improve women pregnant and lying-in women's intestinal flora, but also can by adding maltose, and adjust oligofructose and
The weight ratio and resistant dextrin of inulin and the weight ratio of Lactobacillus rhamnosus, significantly improve probiotics in probiotic composition
Survival rate, extend the pot-life of probiotic composition.The present invention relates to a kind of multifarious composition of improvement intestinal flora,
In particular it relates to a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora.
Contain in the probiotic composition there are many probiotics, various probiotics is made to effectively improve women by interaction
Pregnant and lying-in women's intestinal flora, and probiotic composition prepared by the present invention has the characteristics that high activity, by prolonged normal
It is gentle to put, still maintain higher probiotic active.
Illustrate: all numbers each mean parts by weight in the present invention.
A kind of probiotic composition includes: by weight
40-60 parts of resistant dextrin, 5-15 parts of inulin, 5-15 parts of oligofructose, 2-8 parts of bifidobacterium lactis, rhamnose cream bar
2-10 parts of bacterium, 2-8 parts of Lactobacillus casei, 2-8 parts of lactobacillus acidophilus, 2-10 parts of Lactobacillus helveticus, 2-8 parts of lactobacillus reuteri,
2-10 parts and lactobacillus fermenti 2-10 parts of Lactobacillus rhamnosus;
Preferably, the probiotic composition by weight include: 40-55 parts of resistant dextrin, it is 5-12 parts of inulin, oligomeric
5-12 parts of fructose, 2-6 parts of bifidobacterium lactis, 2-8 parts of Lactobacillus rhamnosus, 2-6 parts of Lactobacillus casei, 2-6 parts of lactobacillus acidophilus,
2-8 parts of Lactobacillus helveticus, 2-8 parts of lactobacillus reuteri, 2-8 parts of Lactobacillus rhamnosus and 2-8 parts of lactobacillus fermenti;
It is further preferred that the probiotic composition includes: 45-55 parts of resistant dextrin, inulin 8-12 by weight
Part, 8-12 parts of oligofructose, 4-6 parts of bifidobacterium lactis, 5-8 parts of Lactobacillus rhamnosus, 4-6 parts of Lactobacillus casei, acidophilus cream bar
4-6 parts of bacterium, 5-8 parts of Lactobacillus helveticus, 5-8 parts of lactobacillus reuteri, 5-8 parts of Lactobacillus rhamnosus and 5-8 parts of lactobacillus fermenti;
In the probiotic composition, the probiotics of addition is added in the form of bacterium powder;
In the probiotic composition, the weight ratio of oligofructose and inulin addition is 1-3:1;Preferably, oligofructose
Weight ratio with inulin addition is 1-1.5:1;It is highly preferred that oligofructose and the weight ratio of inulin addition are 1:1.
In the probiotic composition, the weight ratio of resistant dextrin and Lactobacillus rhamnosus addition is 4-30:1;Preferably,
Resistant dextrin and the weight ratio of Lactobacillus rhamnosus addition are 8-20:1;It is highly preferred that resistant dextrin and Lactobacillus rhamnosus add
The weight ratio added is 3:1.
In some preferred embodiments, maltose 0.5-3 parts is also contained in the probiotic composition;It is some more
In preferred embodiment, maltose 0.5-2.5 parts is also contained in the probiotic composition;
In some preferred embodiments, oligofructose and the weight ratio of inulin are 1:1 in the probiotic composition.
In some preferred embodiments, the weight ratio of resistant dextrin and Lactobacillus rhamnosus in the probiotic composition
For 8-10:1;
Compared with prior art, the invention has the benefit that
(1) probiotic composition of the present invention, wherein can interact between each bacterial strain, to advantageously promote women pregnant
Absorption of puerpera's stomach and intestine to nutriment can increase beneficial to bacterial content, play promotion digestion, stimulate intestine immunity cell, increase
The effects of strong women pregnant and lying-in women's intestinal immunity.
(2) present invention adjusts weight ratio and the oligomeric fruit of resistant dextrin and Lactobacillus rhamnosus by addition maltose
The weight ratio of sugar and inulin, hence it is evident that improve stability of probiotics during preservation in composition.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
The probiotic composition of the embodiment include: by weight 40 parts of resistant dextrin, 5 parts of inulin, 10 parts of oligofructose,
2 parts of bifidobacterium lactis, 2 parts of Lactobacillus rhamnosus, 2 parts of Lactobacillus casei, 8 parts of lactobacillus acidophilus, 10 parts of Lactobacillus helveticus, Roy
8 parts of family name's lactobacillus, 10 parts of Lactobacillus rhamnosus and 10 parts of lactobacillus fermenti;
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder;
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 2:1;
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 9:1.
Embodiment 2
Probiotic composition includes: 50 parts of resistant dextrin, 10 parts of inulin, oligofructose 10 by weight in the embodiment
Part, 5 parts of bifidobacterium lactis, 6 parts of Lactobacillus rhamnosus, 4 parts of Lactobacillus casei, 6 parts of lactobacillus acidophilus, 6 parts of Lactobacillus helveticus, sieve
6 parts of Yi Shi lactobacillus, 6 parts of Lactobacillus rhamnosus and 6 parts of lactobacillus fermenti;
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder;
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 1:1;
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 8.3:1.
Embodiment 3
Probiotic composition includes: 60 parts of resistant dextrin, 10 parts of inulin, oligofructose 15 by weight in the embodiment
Part, 8 parts of bifidobacterium lactis, 10 parts of Lactobacillus rhamnosus, 8 parts of Lactobacillus casei, 2 parts of lactobacillus acidophilus, 2 parts of Lactobacillus helveticus,
2 parts of lactobacillus reuteri, 2 parts of Lactobacillus rhamnosus and 2 parts of lactobacillus fermenti;
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder;
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 1.5:1;
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 6:1;
Embodiment 4
The composition and embodiment 1 of the probiotic composition of the embodiment are identical, only difference is that: the embodiment is in reality
On the basis of the probiotic composition for applying example 1,1.5 parts of maltose are increased.
Comparative example 1
In the embodiment probiotic composition include: by weight 35 parts of resistant dextrin, 4 parts of inulin, 4 parts of oligofructose,
1 part of bifidobacterium lactis, 1 part of Lactobacillus rhamnosus, 1 part of cheese cream bar, 10 parts of lactobacillus acidophilus, 12 parts of Lactobacillus helveticus, Roy
10 parts of family name's lactobacillus, 12 parts of Lactobacillus rhamnosus and 12 parts of lactobacillus fermenti;
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder;
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 1:1;
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 35:1.
Comparative example 2
Probiotic composition includes: 66 parts of resistant dextrin, 16 parts of inulin, oligofructose 16 by weight in the embodiment
Part, 10 parts of bifidobacterium lactis, 12 parts of Lactobacillus rhamnosus, 10 parts of cheese cream bar, 1 part of lactobacillus acidophilus, 1 part of Lactobacillus helveticus,
1 part of lactobacillus reuteri, 1 part of Lactobacillus rhamnosus, 1 part of lactobacillus fermenti;
In the probiotic composition of the embodiment, the probiotics of addition is added in the form of bacterium powder;
In the probiotic composition of the embodiment, the weight ratio of oligofructose and inulin is 1:1;
In the probiotic composition of the embodiment, the weight ratio of resistant dextrin and Lactobacillus rhamnosus is 5.5:1.
The detection of 1 flora abundance of experimental example
The detection of flora abundance: the survey of flora abundance is carried out to the probiotic composition in embodiment 1-4 and comparative example 1-2
Determine method: taking growth conditions good and female rats each 35 in pregnancy period and term, and weight is 250-350g, by 70
Female mice is randomly divided into 7 groups, every group of 5 pregnancy period female mices and 5 term female mices, and totally 10;Wherein 4 groups are experimental group, and 3 groups are pair
It is unified to feed 2 weeks under identical environment according to group, continuous stomach-filling in 15 days then is carried out to female mice, is 20g/kg according to dosage
Stomach-filling is carried out, experimental group carries out stomach-filling using the probiotic composition in 1-4 of the embodiment of the present invention, and control group uses comparative example 1-
Probiotic composition and physiological saline in 2 carry out stomach-filling as blank control and collect the excrement of female mice after stomach-filling in 15 days
Just sample, the 16S rRNA gene for full flora are investigated and analysed, and the abundance of flora in female mice body is detected, and ask average in group
Value, testing result are as shown in table 1;
Illustrate: (1) present invention in lactobacillus acidophilus survival rate, refer to and solid beverage placed 4 under conditions of 25 DEG C
The survival rate of lactobacillus acidophilus after a month;(2) "-" in table of the present invention indicates, this is not present.
Embodiment 1-4 and comparative example 1-2 and blank control are compared, it is found that the present invention passes through in composition
The middle a variety of probiotics of addition can promote the abundance of flora in rat body to 450 or more by 400.
Table 1
The test of 2 probiotics pot-life of experimental example
Probiotic composition in embodiment 1-3 and comparative example 1-2 is carried out pot-life test: 37 DEG C of environment saves
192 hours, and 0 hour, 6 hours, 12 hours, 18 hours, 24 hours, 96 hours 48 hours, 144 hours and 192 small time-divisions
Other to carry out survival volume test to probiotics and average, the results are shown in Table 2;
It can be found that the probiotics survival rate being added in the embodiment 4 of 1.5 portions of maltose is saved by 192 hours
In the process, obviously higher than other embodiments and comparative example;In addition, by embodiment 2 with except the other embodiments of embodiment 4 and right
Discovery when ratio compares, the probiotics survival rate of embodiment 2 are above other embodiments and comparative example except embodiment 4,
That is, applicant is found surprisingly that after adding 1.5 parts of maltose in probiotic composition, probiotic composition
Pot-life it is obviously elongated;In addition, when oligofructose in probiotic composition and the weight ratio of inulin are 1:1, and resist simultaneously
Property dextrin and Lactobacillus rhamnosus weight ratio be 8-10:1 when, the pot-life of probiotic composition is significantly longer.
Table 2
Claims (4)
1. a kind of multifarious probiotic composition of improvement women pregnant and lying-in women's intestinal flora, it is characterised in that include: by weight
40-60 parts of resistant dextrin, 5-15 parts of inulin, 5-15 parts of oligofructose, 2-8 parts of bifidobacterium lactis, 2-10 parts of Lactobacillus rhamnosus,
2-8 parts of Lactobacillus casei, 2-8 parts of lactobacillus acidophilus, 2-10 parts of Lactobacillus helveticus, 2-8 parts of lactobacillus reuteri, rhamnose cream bar
2-10 parts and lactobacillus fermenti 2-10 parts of bacterium;
The oligofructose and the weight ratio of inulin addition are 1-3:1;
The resistant dextrin and the weight ratio of Lactobacillus rhamnosus addition are 4-30:1;
Also contain maltose 0.5-3 parts in the probiotic composition.
2. probiotic composition according to claim 1, which is characterized in that by weight include: resistant dextrin 40-55
Part, 5-12 parts of inulin, 5-12 parts of oligofructose, 2-6 parts of bifidobacterium lactis, 2-8 parts of Lactobacillus rhamnosus, Lactobacillus casei 2-6
Part, 2-6 parts of lactobacillus acidophilus, 2-8 parts of Lactobacillus helveticus, 2-8 parts of lactobacillus reuteri, 2-8 parts of Lactobacillus rhamnosus and fermentation
2-8 parts of lactobacillus.
3. probiotic composition according to claim 2, which is characterized in that by weight include: resistant dextrin 45-55
Part, 8-12 parts of inulin, 8-12 parts of oligofructose, 4-6 parts of bifidobacterium lactis, 5-8 parts of Lactobacillus rhamnosus, Lactobacillus casei 4-6
Part, 4-6 parts of lactobacillus acidophilus, 5-8 parts of Lactobacillus helveticus, 5-8 parts of lactobacillus reuteri, 5-8 parts of Lactobacillus rhamnosus and fermentation
5-8 parts of lactobacillus.
4. probiotic composition as described in any one of claims 1-3, it is characterised in that: the resistant dextrin and rhamnose
The weight ratio of lactobacillus is 8-10:1.
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