CN107647397A - A kind of probiotic powder and preparation method thereof - Google Patents

A kind of probiotic powder and preparation method thereof Download PDF

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Publication number
CN107647397A
CN107647397A CN201710773744.0A CN201710773744A CN107647397A CN 107647397 A CN107647397 A CN 107647397A CN 201710773744 A CN201710773744 A CN 201710773744A CN 107647397 A CN107647397 A CN 107647397A
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parts
powder
probiotic
tea
weight
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程彦
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Guangzhou Funuo Nutrition Technology Co Ltd
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Guangzhou Funuo Nutrition Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/166—Addition of, or treatment with, enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/125—Casei
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/155—Kefiri

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  • Health & Medical Sciences (AREA)
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  • Polymers & Plastics (AREA)
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  • Nutrition Science (AREA)
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Abstract

The invention belongs to food technology field, and in particular to a kind of probiotic powder and preparation method thereof.Probiotic powder of the present invention includes following components and its parts by weight：32 46 parts of 8 16 parts of Pu'er tea ferment powder, 10 18 parts of polynary bacterium powder, 68 parts of soybean separation protein white powder, 6 12 parts of spirulina powder, 12 20 parts of modified yeast glucan, 26 parts of black bamboo salt, 48 parts of honey and FOS.Probiotic powder compatibility provided by the invention is reasonable, science, and the interphase interaction of each component, not only mouthfeel is good, stability is good, but also has and relax bowel, the effect of toxin expelling, lipid-lowering weight-reducing.

Description

A kind of probiotic powder and preparation method thereof
Technical field
The invention belongs to food technology field, and in particular to a kind of probiotic powder and preparation method thereof.
Background technology
Because the factors such as the quickening of people's rhythm of life, diet, medicine effect cause inferior health problem increasingly to highlight, groups of people Group is due to the daily food for having taken in more higher fatty acid, high protein and high heat, and amount of exercise is relatively fewer, causes nutrition The accumulation of toxin and impurity is serious in surplus and blood, and immunity also declines therewith, the ill symptomses such as obesity, constipation occurs, Very big puzzlement is brought to live and work.
Probiotics is also known as active bacteria formulation, microecologic regulator, regulator etc., is to be colonized in human body intestinal canal, in reproductive system, Definite health efficacy can be produced so as to the active beneficial microorganism general name for improving host's microecological balance, playing beneficial effect. It is usually used at present in food, medicine, health products and animal feed, probiotics is in prevention and treatment diarrhoea, constipation, improvement stomach work( Can, reducing toxins in human body content etc. has special effect, and can also play improves intestinal microflora, promotes enteron aisle The propagation of middle beneficial bacterium, the growth for suppressing harmful bacteria, improve the effects such as immunity of organisms.In addition, research has shown that Pu'er tea to drop Low blood fat, cholesterol, preventing and treating obesity, hypertension, worry illness etc. have significant role, and with these healthcare function quilts Science confirms that Pu'er tea is increasingly subject to the favor of people one by one, and titled with the laudatory title such as " slim tea ", " gentle and graceful tea ", " lengthen one's life tea ".
Chinese patent application CN106036718A discloses a kind of probiotics coconut walnut powder and preparation method thereof, the benefit Raw bacterium coconut walnut powder is by coconut, walnut kernel, bifidobacterium lactis powder, lactobacillus reuteri powder, lactobacillus bulgaricus, thermophilic chain The raw materials such as coccus, cassia seed are made, and the probiotics coconut walnut powder can improve intestinal environment, beneficial to intestines and stomach.
Chinese patent application CN104473183A discloses a kind of fat-reducing probiotic tablet and preparation method thereof, the fat-reducing Probiotic tablet is using probiotics powder agents such as Lactobacillus plantarums as primary raw material, and composite modified dietary fiber, fruit vegetable powder, albumen Powder, Chinese medical extract etc., it fully have adjusted the composition of beneficial bacteria of intestinal tract flora, the fat-reducing probiotic tablet bioactivity of preparation It is high, extensive in human body intestinal canal resident time length, the notable simultaneously suitable population of fat-reducing effect.
But it is now conventional relax bowel or diet products in largely containing excitants such as cassia seed, folium sennae, rheum officinales Purgatives, neuron in intestinal wall is stimulated to cause enterocinesia to increase, it is long-term use of although rapid defaecation, the purpose of fat-reducing can be reached Drug dependence is easily formed, impairing the spleen and stomach is totally unfavorable to human body.In addition, the probiotics flora only to live is just active, Conditioning enteron aisle can just be played, improve immunity and other effects.Therefore during production and preservation, how farthest to ensure to live Bacterium survival rate is also to have to solve the problems, such as.
The content of the invention
In order to solve problems of the prior art, it is an object of the invention to provide a kind of probiotic powder and its preparation Method.Probiotic powder provided by the invention is not only convenient to take, mouthfeel it is pure it is good, stability is good, but also with relaxing bowel, drop The effect of fat weight reducing.
The invention provides a kind of probiotic powder, including following components and its parts by weight：
Pu'er tea ferment powder 8-16 parts, polynary bacterium powder 10-18 parts, soybean separation protein white powder 6-8 parts, spirulina powder 6-12 Part, modified yeast glucan 12-20 parts, black bamboo salt 2-6 parts, honey 4-8 parts and FOS 32-46 parts.
Further, the probiotic powder, is made up of following components and its parts by weight：
12 parts of Pu'er tea ferment powder, 14 parts of polynary bacterium powder, 7 parts of soybean separation protein white powder, 9 parts of spirulina powder, modified yeast 39 parts of 16 parts of glucan, 4 parts of black bamboo salt, 6 parts of honey and FOS.
Further, the preparation method of the Pu'er tea ferment powder is：
(1) fresh Pu'er tea is added in the ethanol water for being 50% to volumetric concentration, it is ultrasonic under the conditions of 30-40 DEG C 7-10min, Pu'er tea-alcohol mixture is obtained, it is quick-fried for 0.5-1.2MPa steam that above-mentioned Pu'er tea-alcohol mixture is placed in into pressure In broken tank, 100 DEG C of holding 1-2min, the quick release pressure in 1-3s, solidliquid mixture is obtained；
(2) orange streptomycete and Kefir grains lactobacillus are sequentially ingressed into culture medium, 25-30 DEG C of standing 0.5-1h, then White ginseng strain is accessed, obtains leavening；
(3) solidliquid mixture obtained by step (1) is added into leavening obtained by step (2), 25-38 DEG C of fermentation 10-15 My god, centrifugation, the filtering of 100-150 mesh, concentrate, spray drying, produce.
Further, weight ratio of the fresh Pu'er tea with volumetric concentration for 50% ethanol water in the step (1) For 1：(2-8).
Further, culture medium is made up of following components and its parts by weight in the step (2)：Albumen powder 30-48 parts, Xylitol 5-20 parts and potassium dihydrogen phosphate 8-15 parts.
Further, the access amount of orange streptomycete is 1 × 10 in the step (2)7-1×108Cfu/g, Kefir grains breast The access amount of bacillus is 1 × 106-1×107The weight ratio of cfu/g, white ginseng bacterium and culture medium is (1-2):(100-150).
Further, the polynary bacterium powder by clostridium butyricum powder, lactobacillus acidophilus powder and Lactobacillus casei powder by weight (1-2)：(3-4)：(0.5-1) is formed.
Further, the preparation method of the modified yeast glucan is：
1) by xanthans and water by weight (0.2-1.5)：(0.5-2) is mixed, in stir speed (S.S.) 500-800r/min bars 15-30min is reacted under part, obtains xanthan gum solution；
2) yeast dextran after grinding is added in xanthan gum solution obtained by step 1), pre-gelatinized, vacuum at -20 DEG C 1-2h is freeze-dried, pulverizes and sieves, produces.
In addition, present invention also offers a kind of preparation method of probiotic powder, comprise the following steps：
S1, by Pu'er tea ferment powder, soybean separation protein white powder, spirulina powder, modified yeast glucan, black bamboo salt, honey Three-dimensional motion mixer mixing 15-20min is added with FOS, spray drying, water content is less than 3%, homogeneous, crosses 100- 120 mesh sieves, obtain premix；
S2, polynary bacterium powder added in premix obtained by step S1, in being well mixed in mixer, produced.
The present invention is manufactured separately to obtain Pu'er tea ferment powder first, and inoculation white ginseng bacterium effectively reduces the bitter taste of tea, then The compositions such as science compounded combination live probiotic bacterium powder, the high temperature in existing process pulverizing process can be avoided to lose probiotics and lived Property the problem of, farthest retain probiotics vigor；In addition, Pu'er tea ferment has good complementary work with probiotics With ferment plays a part of the prebiotic factor, can promote the breeding of probiotics in human body intestinal canal, and probiotics can also strengthen ferment Has the effect of use characteristic；The present invention is modified to yeast dextran, and its water solubility enhancing, the FOS of use is a kind of water Soluble dietary fiber, there is very strong water suction binding ability, can increase the volume of excrement in enteron aisle, accelerate transhipment drainage rate, The harmful substance contact intestinal wall time is reduced, and there is synergy, cooperation between the two between probiotics and FOS Effect is the co between the probiotic in symphysis unit and prebiotics, and they complement each other, and collectively promotes beneficial bacterium life Long propagation, and suppress the growth of harmful bacteria.
Compared with prior art, the technical advantages of the present invention are that：Probiotic powder provided by the invention is without irritant Purgatives, rich in function factors such as Tea Polyphenols, caffeine, tea Chinese honey locusts in Pu'er tea ferment powder, with the composition such as probiotics, FOS Collective effect, human body intestinal canal Tiny ecosystem can be adjusted well, promotes the wriggling of enteron aisle, prevents constipation, reach catharsis and toxin expelling, drop The effect of fat is reduced weight.In addition, probiotic powder preparation technology provided by the invention is simple, it can directly take or warm water reconstitutes, It is in good taste.
Embodiment
Below by way of the description of embodiment, the invention will be further described, but this is not the limit to the present invention System, those skilled in the art according to the present invention basic thought, various modifications may be made or improve, but without departing from this The basic thought of invention, within the scope of the present invention.
In the present invention, soybean separation protein white powder is purchased from Henan Zhenhua Fine Chemical Co., Ltd, food-grade；Yeast Portugal gathers Sugar is purchased from Shaanxi Sen Fu natural products Co., Ltd, food-grade；Black bamboo salt is purchased from Zibo Zheng Bang Bioisystech Co., Ltd, Food-grade；Honey is purchased from the great space plantation farmer specialized cooperative society in Guyuan City Yuanzhou District, model：hyhzs2016；Spirulina powder No. CAS：724424-92-4；FOS CAS：308066-66-2；Xanthans CAS：11138-66-2.
Embodiment 1, a kind of probiotic powder
The probiotic powder is made up of following components and its parts by weight：
8 parts of Pu'er tea ferment powder, 10 parts of polynary bacterium powder, 6 parts of soybean separation protein white powder, 6 parts of spirulina powder, modified yeast Portugal 32 parts of 12 parts of glycan, 2 parts of black bamboo salt, 4 parts of honey and FOS.
The polynary bacterium powder is by clostridium butyricum powder, lactobacillus acidophilus powder and Lactobacillus casei powder by weight 1：3：1 composition.
The preparation method of the Pu'er tea ferment powder is：
(1) fresh Pu'er tea is added in the ethanol water for being 50% to volumetric concentration, it is ultrasonic under the conditions of 30 DEG C 7min, Pu'er tea-alcohol mixture is obtained, above-mentioned Pu'er tea-alcohol mixture is placed in pressure as in 0.5MPa steam blasting tanks, 100 DEG C of holding 1min, the quick release pressure in 3s, obtain solidliquid mixture；
(2) orange streptomycete and Kefir grains lactobacillus are sequentially ingressed into culture medium, 25 DEG C of standing 0.5h, then accessed white Join strain, obtain leavening；
(3) solidliquid mixture obtained by step (1) is added into leavening obtained by step (2), 25 DEG C ferment 10 days, from The heart, 100 mesh filtering, is concentrated, and spray drying, is produced.
The weight ratio for the ethanol water that fresh Pu'er tea is 50% with volumetric concentration is 1 in the step (1)：2.
Culture medium is made up of following components and its parts by weight in the step (2)：30 parts of albumen powder, 5 parts of xylitol and 8 parts of potassium dihydrogen phosphate.
The access amount of orange streptomycete is 1 × 10 in the step (2)7Cfu/g, the access amount of Kefir grains lactobacillus is 1 ×106The weight ratio of cfu/g, white ginseng bacterium and culture medium is 1：100.
The preparation method of the modified yeast glucan is：
1) by xanthans and water by weight 0.2：1 mixing, reacts 15min under the conditions of stir speed (S.S.) 500r/min, obtains Xanthan gum solution；
2) yeast dextran after grinding is added in xanthan gum solution obtained by step 1), pre-gelatinized, vacuum at -20 DEG C 1h is freeze-dried, pulverizes and sieves, produces.
Preparation method：
S1, by Pu'er tea ferment powder, soybean separation protein white powder, spirulina powder, modified yeast glucan, black bamboo salt, honey Three-dimensional motion mixer mixing 15min is added with FOS, spray drying, water content is less than 3%, homogeneous, crosses 100 mesh Sieve, obtains premix；
S2, polynary bacterium powder added in premix obtained by step S1, in being well mixed in mixer, produced.
Embodiment 2, a kind of probiotic powder
The probiotic powder is made up of following components and its parts by weight：
12 parts of Pu'er tea ferment powder, 14 parts of polynary bacterium powder, 7 parts of soybean separation protein white powder, 9 parts of spirulina powder, modified yeast 39 parts of 16 parts of glucan, 4 parts of black bamboo salt, 6 parts of honey and FOS.
The polynary bacterium powder is by clostridium butyricum powder, lactobacillus acidophilus powder and Lactobacillus casei powder by weight 2：4：1 composition.
The preparation method of the Pu'er tea ferment powder is：
(1) fresh Pu'er tea is added in the ethanol water for being 50% to volumetric concentration, it is ultrasonic under the conditions of 35 DEG C 9min, Pu'er tea-alcohol mixture is obtained, above-mentioned Pu'er tea-alcohol mixture is placed in pressure as in 0.8MPa steam blasting tanks, 100 DEG C of holding 1.5min, the quick release pressure in 3s, obtain solidliquid mixture；
(2) orange streptomycete and Kefir grains lactobacillus are sequentially ingressed into culture medium, 27 DEG C of standing 1h, then access white ginseng Strain, obtain leavening；
(3) solidliquid mixture obtained by step (1) is added into leavening obtained by step (2), 35 DEG C ferment 12 days, from The heart, 120 mesh filtering, is concentrated, and spray drying, is produced.
The weight ratio for the ethanol water that fresh Pu'er tea is 50% with volumetric concentration is 1 in the step (1)：5.
Culture medium is made up of following components and its parts by weight in the step (2)：40 parts of albumen powder, 12 parts of xylitol and 10 parts of potassium dihydrogen phosphate.
The access amount of orange streptomycete is 0.5 × 10 in the step (2)8Cfu/g, the access amount of Kefir grains lactobacillus are 0.5×107The weight ratio of cfu/g, white ginseng bacterium and culture medium is 1.5：130.
The preparation method of the modified yeast glucan is：
1) by xanthans and water by weight 0.5：2 mixing, react 20min under the conditions of stir speed (S.S.) 700r/min, obtain Xanthan gum solution；
2) yeast dextran after grinding is added in xanthan gum solution obtained by step 1), pre-gelatinized, vacuum at -20 DEG C 1.5h is freeze-dried, pulverizes and sieves, produces.
Preparation method：
S1, by Pu'er tea ferment powder, soybean separation protein white powder, spirulina powder, modified yeast glucan, black bamboo salt, honey Three-dimensional motion mixer mixing 18min is added with FOS, spray drying, water content is less than 3%, homogeneous, crosses 120 mesh Sieve, obtains premix；
S2, polynary bacterium powder added in premix obtained by step S1, in being well mixed in mixer, produced.
Embodiment 3, a kind of probiotic powder
The probiotic powder is made up of following components and its parts by weight：
16 parts of Pu'er tea ferment powder, 18 parts of polynary bacterium powder, 8 parts of soybean separation protein white powder, 12 parts of spirulina powder, modified yeast 46 parts of 20 parts of glucan, 6 parts of black bamboo salt, 8 parts of honey and FOS.
The polynary bacterium powder is by clostridium butyricum powder, lactobacillus acidophilus powder and Lactobacillus casei powder by weight 2：3：1 composition.
The preparation method of the Pu'er tea ferment powder is：
(1) fresh Pu'er tea is added in the ethanol water for being 50% to volumetric concentration, it is ultrasonic under the conditions of 40 DEG C 10min, Pu'er tea-alcohol mixture is obtained, it is 1.2MPa steam blasting tanks that above-mentioned Pu'er tea-alcohol mixture is placed in into pressure In, 100 DEG C of holding 2min, the quick release pressure in 3s, obtain solidliquid mixture；
(2) orange streptomycete and Kefir grains lactobacillus are sequentially ingressed into culture medium, 30 DEG C of standing 1h, then access white ginseng Strain, obtain leavening；
(3) solidliquid mixture obtained by step (1) is added into leavening obtained by step (2), 38 DEG C ferment 15 days, from The heart, 150 mesh filtering, is concentrated, and spray drying, is produced.
The weight ratio for the ethanol water that fresh Pu'er tea is 50% with volumetric concentration is 1 in the step (1)：8.
Culture medium is made up of following components and its parts by weight in the step (2)：48 parts of albumen powder, 20 parts of xylitol and 15 parts of potassium dihydrogen phosphate.
The access amount of orange streptomycete is 1 × 10 in the step (2)8Cfu/g, the access amount of Kefir grains lactobacillus is 1 ×107The weight ratio of cfu/g, white ginseng bacterium and culture medium is 2：150.
The preparation method of the modified yeast glucan is：
1) by xanthans and water by weight 1.5：2 mixing, react 30min under the conditions of stir speed (S.S.) 800r/min, obtain Xanthan gum solution；
2) yeast dextran after grinding is added in xanthan gum solution obtained by step 1), pre-gelatinized, vacuum at -20 DEG C 2h is freeze-dried, pulverizes and sieves, produces.
Preparation method：
S1, by Pu'er tea ferment powder, soybean separation protein white powder, spirulina powder, modified yeast glucan, black bamboo salt, honey Three-dimensional motion mixer mixing 20min is added with FOS, spray drying, water content is less than 3%, homogeneous, crosses 120 mesh Sieve, obtains premix；
S2, polynary bacterium powder added in premix obtained by step S1, in being well mixed in mixer, produced.
Comparative example 1, a kind of probiotic powder
The probiotic powder is made up of following components and its parts by weight：
12 parts of Pu'er tea ferment powder, 14 parts of polynary bacterium powder, 7 parts of soybean separation protein white powder, 9 parts of spirulina powder, modified yeast 39 parts of 16 parts of glucan, 4 parts of black bamboo salt, 6 parts of honey and FOS.
The polynary bacterium powder is by clostridium butyricum powder, lactobacillus acidophilus powder and Lactobacillus casei powder by weight 2：4：1 composition.
White ginseng bacterium in the preparation method of the Pu'er tea ferment powder in step (2) replaces with bafillus natto, access Measure as 1 × 107Cfu/g, remaining step are same as Example 2.
The preparation method of the modified yeast glucan is similar to Example 2.
The preparation method of the probiotic powder is similar to Example 2.
Difference with embodiment 2 is, white ginseng bacterium is replaced with into bafillus natto, access amount is 1 × 107cfu/g。
Comparative example 2, a kind of probiotic powder
The probiotic powder is made up of following components and its parts by weight：
12 parts of Pu'er tea ferment powder, 14 parts of polynary bacterium powder, 7 parts of soybean separation protein white powder, 9 parts of spirulina powder, modified yeast 39 parts of 16 parts of glucan, 4 parts of black bamboo salt, 6 parts of honey and FOS.
The polynary bacterium powder is by clostridium butyricum powder, lactobacillus acidophilus powder and Lactobacillus casei powder by weight 2：4：1 composition.
The preparation method of the Pu'er tea ferment powder is similar to Example 2.
Xanthans is replaced with into guar gum in the preparation method of the modified yeast glucan, remaining step and embodiment 2 is identical.
The preparation method of the probiotic powder is similar to Example 2.
Difference with embodiment 2 is, xanthans is replaced with into guar gum in the preparation method of modified yeast glucan.
Comparative example 3, a kind of probiotic powder
The probiotic powder is made up of following components and its parts by weight：
12 parts of Pu'er tea ferment powder, 14 parts of polynary bacterium powder, 7 parts of soybean separation protein white powder, 9 parts of spirulina powder, modified yeast 39 parts of 16 parts of glucan, 4 parts of black bamboo salt, 6 parts of honey and FOS.
The polynary bacterium powder is by clostridium butyricum powder and lactobacillus acidophilus powder by weight 3：4 compositions.
The preparation method of the Pu'er tea ferment powder is similar to Example 2.
The preparation method of the modified yeast glucan is similar to Example 2.
The preparation method of the probiotic powder is similar to Example 2.
Difference with embodiment 2 is, Lactobacillus casei powder is free of in polynary bacterium powder, i.e., polynary bacterium powder is by clostridium butyricum powder With lactobacillus acidophilus powder by weight 3：4 compositions.
Test example one, relax bowel experiment
1. test material：Probiotic powder prepared by embodiment 1-3 and comparative example 3.
2. subjects：Male Kunming strain mice 80, body weight 20-25g.
3. test method：
(1) mouse Intestinal pushing acts on
40 mouse are extracted, are randomly divided into 4 groups, every group 10, respectively blank control group, embodiment 1-3 groups and contrast 3 groups of example, the aqueous solution 5mL that the probiotic powder prepared with distilled water 5mL, embodiment 1-3 and comparative example 3 reconstitutes is filled respectively (0.05g/mL), once a day, normally fed during experiment, it is continuous to feed 15 days, last day fasting 24h, then with 0.5mL 10% Prepared Chinese ink gavage, cervical vertebra is taken off after 20min and puts to death mouse, abdomen is opened and takes small intestine, cut off upper end to pylorus, the intestines of lower end to ileocecus Pipe, gently pulls into straight line, and measurement prepared Chinese ink promotes length and total small intestinal length, calculates ink progradation.Ink progradation (%)= (prepared Chinese ink promotes length/total small intestinal length) × 100%.
(2) mouse defecation situation
40 mouse are extracted, are randomly divided into 4 groups, every group 10, respectively blank control group, embodiment 1-3 groups and contrast 3 groups of example, the aqueous solution 5mL that the probiotic powder prepared with distilled water 5mL, embodiment 1-3 and comparative example 3 reconstitutes is filled respectively (0.05g/mL), once a day, normally to be fed during experiment, continuous single cage is fed 15 days, last day fasting 12h, then with 0.5mL10% prepared Chinese ink gavage, mouse is put into cage, and filter paper is lined with cage, and record mouse is arranged in melena time and 5h first Feces volume.4. result of the test：Test result is as shown in table 1.
Table 1 relaxes bowel measurement result
Classification Ink progradation (%) The melena time (min) is arranged first Feces volume (g)
Blank control group 42.8±8.3 104.8±21.7 0.257±0.075
1 group of embodiment 61.4±7.6**a 72.6±14.3**a 0.356±0.049**a
2 groups of embodiment 62.1±8.5**a 61.2±9.6**aa 0.395±0.056**a
3 groups of embodiment 59.2±14.2* 64.3±17.1**a 0.349±0.042**a
3 groups of comparative example 52.7±6.3* 87.4±10.2* 0.295±0.035*
Note：* with blank control group ratio, there is significant difference (P ＜ 0.05)；* and blank control group ratio, there is pole conspicuousness Difference (P ＜ 0.01)；A and 3 groups of ratios of comparative example, there is significant difference (P ＜ 0.05).
As shown in Table 1, the probiotic powder for preparing of the present invention relaxes bowel significant effect to mouse.With blank control group phase Than progradation of the probiotic powder of the present invention to mouse intestines has conspicuousness, has the effect of promoting defecation, toxin expelling, wherein implementing The effect of example 2 is best, is highly preferred embodiment of the present invention；Compared with 3 groups of comparative example, the probiotics of the preparation of the embodiment of the present invention 2 Powder relaxes bowel to mouse, effect of ejecting poison is notable, illustrates that probiotic powder formula prepared by the present invention is reasonable.
Test example two, lipid-loweringing experiment
1. test material：Probiotic powder prepared by embodiment 1-3 and comparative example 3.
2. subjects：Kunming mouse 40 (male and female half and half), body weight 40-50g.
3. test method：
Mouse is randomly divided into 4 groups, every group 10, respectively 3 groups of blank control group, embodiment 1-3 groups and comparative example, divided The molten aqueous solution 5mL (0.05g/mL) reconstituted of probiotic powder prepared with distilled water 5mL, embodiment 1-3 and comparative example 3 is not filled, Once a day, normally fed during experiment, it is continuous to feed 50 days, last day fasting 12h, eye is plucked under etherization and takes blood, blood sample Serum is separated after standing 2h at room temperature, T-CHOL (TC), triglyceride in serum are determined using automatic clinical chemistry analyzer (TG) and low density cholesterol ester (LDL-C) content.
4. result of the test：TC, TG and LDL-C content are as shown in table 2 in blood.
Table 2 TC, TG and LDL-C assay result
Classification TC(mmol/L) TG(mmol/L) LDL-C(mmol/L)
Blank control group 1.82±0.22 1.61±0.16 1.72±0.15
1 group of embodiment 1.64±0.15* 1.39±0.09**a 1.51±0.19*
2 groups of embodiment 1.57±0.12**a 1.32±0.11**a 1.43±0.12**a
3 groups of embodiment 1.62±0.13* 1.38±0.15* 1.54±0.16*
3 groups of comparative example 1.72±0.15 1.52±0.07* 1.64±0.15
Note：* with blank control group ratio, there is significant difference (P ＜ 0.05)；* and blank control group ratio, there is pole conspicuousness Difference (P ＜ 0.01)；A and 3 groups of ratios of comparative example, there is significant difference (P ＜ 0.05).
As shown in Table 2, compared with blank control group, probiotic powder prepared by the present invention is to TC, TG and LDL-C in Mice Body Content has different degrees of reduction effect, illustrate that probiotic powder of the present invention has an obvious lipid-lowering effect, wherein embodiment 2 Lipid-lowering effect is best, is highly preferred embodiment of the present invention；Compared with 3 groups of comparative example, the probiotic powder of the preparation of the embodiment of the present invention 2 Lipid-lowering effect it is more preferable, and difference has conspicuousness.
Test example three, stability test
1. test material：Probiotic powder prepared by embodiment 1-3.
2. test method：
The probiotic powder 5g that respectively prepared by Example 1-3, according to GB/T 4789-2016, " food hygiene microbiology is examined Test " and paper " stability study of probiotics powder agent product " in method measure clostridium butyricum, lactobacillus acidophilus and cheese breast The number of viable of bacillus, then place 2 months in the incubator that temperature is 38-40 DEG C, humidity is 50-60%, determine again The number of viable of clostridium butyricum, lactobacillus acidophilus and Lactobacillus casei, calculate Viable detection.
3. result of the test：Stability test result is as shown in table 3.
The Viable detection measurement result of table 3
Classification Clostridium butyricum (%) Lactobacillus acidophilus (%) Lactobacillus casei (%)
Embodiment 1 82.6 78.3 80.2
Embodiment 2 89.5 80.5 84.5
Embodiment 3 84.7 76.6 79.4
As shown in Table 3, the probiotic powder that prepared by 1-3 of the embodiment of the present invention after specially treated in 2 months, deposit by probiotics Motility rate is higher, shows that its shelf stability is preferable.
Test example four, sensory evaluation
1. test material：Probiotic powder prepared by embodiment 1-3 and comparative example 1-3.
2. subjects：Each 60 people of 25-40 year men and women.
3. test method：
Subject is randomly divided into 6 groups, every group of 20 people.Each group drinks the embodiment 1-3 reconstituted through warm water and contrast respectively Probiotic powder (0.05g/mL) prepared by example 1-3, to its dissolubility, appearance luster and drink mouthfeel after 30 days and evaluate, Standards of grading：Using ten point system, 10 points best, and 0 point worst, and fraction is higher, and representative evaluation is better.
4. result of the test：Analyses Methods for Sensory Evaluation Results is as shown in table 4.
The Analyses Methods for Sensory Evaluation Results of table 4
Group Dissolubility Appearance luster Mouthfeel
Embodiment 1 8.9 8.7 9.3
Embodiment 2 9.3 9.2 9.4
Embodiment 3 9.0 8.9 8.6
Comparative example 1 8.4 7.2 7.9
Comparative example 2 7.8 8.0 8.8
Comparative example 3 8.5 8.3 8.4
As shown in Table 4, the probiotic powder dissolubility that prepared by the present invention, appearance luster and drink good in taste, illustrate this hair Bright formula is relatively reasonable, and wherein embodiment 2 is highly preferred embodiment of the present invention；Compared with comparative example 1-3, the embodiment of the present invention 2 The sensory evaluation of the probiotic powder of preparation is more preferable.

Claims (9)

1. a kind of probiotic powder, it is characterised in that including following components and its parts by weight：
Pu'er tea ferment powder 8-16 parts, polynary bacterium powder 10-18 parts, soybean separation protein white powder 6-8 parts, spirulina powder 6-12 parts, change Property yeast dextran 12-20 parts, black bamboo salt 2-6 parts, honey 4-8 parts and FOS 32-46 parts.
2. probiotic powder according to claim 1, it is characterised in that be made up of following components and its parts by weight：
12 parts of Pu'er tea ferment powder, 14 parts of polynary bacterium powder, 7 parts of soybean separation protein white powder, 9 parts of spirulina powder, modified yeast Portugal gather 39 parts of 16 parts of sugar, 4 parts of black bamboo salt, 6 parts of honey and FOS.
3. probiotic powder according to claim 1 or 2, it is characterised in that the preparation method of the Pu'er tea ferment powder is：
(1) fresh Pu'er tea is added in the ethanol water for being 50% to volumetric concentration, the ultrasonic 7- under the conditions of 30-40 DEG C 10min, Pu'er tea-alcohol mixture is obtained, it is 0.5-1.2MPa steam blastings that above-mentioned Pu'er tea-alcohol mixture is placed in into pressure In tank, 100 DEG C of holding 1-2min, the quick release pressure in 1-3s, solidliquid mixture is obtained；
(2) orange streptomycete and Kefir grains lactobacillus are sequentially ingressed into culture medium, 25-30 DEG C of standing 0.5-1h, then accessed White ginseng strain, obtains leavening；
(3) solidliquid mixture obtained by step (1) being added into leavening obtained by step (2), 25-38 DEG C ferments 10-15 days, from The heart, 100-150 mesh filtering, is concentrated, and spray drying, is produced.
4. probiotic powder according to claim 3, it is characterised in that fresh Pu'er tea and volume are dense in the step (1) The weight ratio for spending the ethanol water for 50% is 1：(2-8).
5. probiotic powder according to claim 3, it is characterised in that in the step (2) culture medium by following components and Its parts by weight forms：Albumen powder 30-48 parts, xylitol 5-20 parts and potassium dihydrogen phosphate 8-15 parts.
6. probiotic powder according to claim 3, it is characterised in that the access amount of orange streptomycete in the step (2) For 1 × 107-1×108Cfu/g, the access amount of Kefir grains lactobacillus is 1 × 106-1×107Cfu/g, white ginseng bacterium and culture medium Weight ratio is (1-2):(100-150).
7. probiotic powder according to claim 1 or 2, it is characterised in that the polynary bacterium powder is by clostridium butyricum powder, acidophilus Lactobacillus powder and Lactobacillus casei powder are by weight (1-2)：(3-4)：(0.5-1) is formed.
8. probiotic powder according to claim 1 or 2, it is characterised in that the preparation method of the modified yeast glucan For：
1) by xanthans and water by weight (0.2-1.5)：(0.5-2) is mixed, under the conditions of stir speed (S.S.) 500-800r/min 15-30min is reacted, obtains xanthan gum solution；
2) yeast dextran after grinding is added in xanthan gum solution obtained by step 1), pre-gelatinized, vacuum refrigeration at -20 DEG C 1-2h is dried, pulverizes and sieves, produces.
9. the preparation method of the probiotic powder according to claim any one of 1-8, it is characterised in that comprise the following steps：
S1, by Pu'er tea ferment powder, soybean separation protein white powder, spirulina powder, modified yeast glucan, black bamboo salt, honey and low Fructooligosaccharides adds three-dimensional motion mixer mixing 15-20min, spray drying, water content is less than 3%, homogeneous, crosses 100-120 Mesh sieve, obtain premix；
S2, polynary bacterium powder added in premix obtained by step S1, in being well mixed in mixer, produced.
CN201710773744.0A 2017-08-31 2017-08-31 A kind of probiotic powder and preparation method thereof Pending CN107647397A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110025639A (en) * 2019-03-07 2019-07-19 浙江工业大学 Application of the yeast dextran composition in prevent constipation
CN110537596A (en) * 2019-08-16 2019-12-06 大别山野岭饮料股份有限公司 Enzyme tea and production process thereof
JP2022024959A (en) * 2020-07-16 2022-02-09 オクスロ株式会社 Bifidobacteria bz25 fermented milk having cholesterol reducing effect

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Publication number Priority date Publication date Assignee Title
CN102422934A (en) * 2011-11-11 2012-04-25 云南省农业科学院茶叶研究所 Method for preparing schizophyllumcommuneh fermented tea
CN104413334A (en) * 2013-08-30 2015-03-18 深圳华大基因科技有限公司 Edible composition as well as preparation method and application thereof
CN106418501A (en) * 2016-09-09 2017-02-22 杨森健 Healthcare food with weight reducing function

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102422934A (en) * 2011-11-11 2012-04-25 云南省农业科学院茶叶研究所 Method for preparing schizophyllumcommuneh fermented tea
CN104413334A (en) * 2013-08-30 2015-03-18 深圳华大基因科技有限公司 Edible composition as well as preparation method and application thereof
CN106418501A (en) * 2016-09-09 2017-02-22 杨森健 Healthcare food with weight reducing function

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110025639A (en) * 2019-03-07 2019-07-19 浙江工业大学 Application of the yeast dextran composition in prevent constipation
CN110537596A (en) * 2019-08-16 2019-12-06 大别山野岭饮料股份有限公司 Enzyme tea and production process thereof
JP2022024959A (en) * 2020-07-16 2022-02-09 オクスロ株式会社 Bifidobacteria bz25 fermented milk having cholesterol reducing effect
JP7090144B2 (en) 2020-07-16 2022-06-23 オクスロ株式会社 Bifidobacterium BZ25 fermented milk with cholesterol lowering effect

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