CN108935709A - A kind of probiotics goat milk piece and preparation method thereof - Google Patents
A kind of probiotics goat milk piece and preparation method thereof Download PDFInfo
- Publication number
- CN108935709A CN108935709A CN201810779974.2A CN201810779974A CN108935709A CN 108935709 A CN108935709 A CN 108935709A CN 201810779974 A CN201810779974 A CN 201810779974A CN 108935709 A CN108935709 A CN 108935709A
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- probiotics
- goat milk
- powder
- freeze
- cream
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Classifications
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
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- A23C9/156—Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
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Abstract
A kind of probiotics goat milk piece and preparation method thereof; it is added to proliferation factor, the anti-freezing factor and freeze drying protectant of the integration of drinking and medicinal herbs date polysaccharide as probiotics in the medium; promote the proliferation and its frost resistance of probiotics; it is subjected to cold treatment at low temperature again; the antifreeze protein that induction generates further increases its frost resistance; cryohydric point is reduced by compound freeze drying protectant again and increases the protective effect to probiotics, to obtain high-activity probiotics bacterium powder.The number of live bacteria of probiotics added in probiotics goat milk piece of the invention is high, consumer's beneficial bacteria of intestinal tract quantity can be improved and adjust colony balance, the dietary fiber glucan and xylo-oligosaccharide of addition can promote the proliferation of beneficial bacteria of intestinal tract and the absorption of minerals and increase intestines peristalsis, prevent constipation, the functional sweetener of addition have it is low in calories, not will lead to saprodontia and obesity, on blood glucose also without influence, and have the function of relax bowel and defecation and promote calcium uptake etc..
Description
Technical field
The present invention relates to a kind of functional goat milk pieces and preparation method thereof, and in particular to one kind contains probiotics
(Probiotics), dietary fiber and the probiotics goat milk piece of functional sweetener and preparation method thereof.
Background technique
Goat milk has following nutritive peculiarity: (1) protein, which forms protein in reasonable goat milk, mainly casein and whey
Albumen composition, the ratio between goat milk, milk, the casein in people's milk and lactalbumin are about are as follows: 75:25,80:20,60:40, group
At closer to breast milk, because casein content is relatively low in sheep cream, formation curds mass is small and relatively soft under one's belt, suction easy to digest
It receives, so the digestibility of sheep milk protein is higher than milk, is more suitable for baby and old man drinks.(2) protein forms reasonable goat milk
Protein is not easy the α in allergy milkS1Casein and beta lactoglobulin are anaphylactogens generally acknowledged at present, can be caused allergic reaction, out
The adverse reactions such as existing fash, eczema and abdominal pain diarrhea.α in milkS1Casein accounts for the 43% of casein, and 1- is only accounted in goat milk
3%, therefore drink goat milk and be not easy allergy (3) protein and form the reasonable goat milk fat fat globule easy to digest absorbed in goat milk
It is 3.49 μm, cow's milk fat globule is 4.55 μm, since the fatty bulb diameter of goat milk is small, is easy to be digested, digestibility is up to 97-
98%, while the fatty acid in goat milk is not easy to accumulate in human body based on middle chain and short chain, one, U.S. scientist studies table
Bright, goat milk fat is mainly consumed in the form of heat in body metabolism, the interior accumulation of human body will not generate puffiness phenomenon again.(4)
It is the main source that people absorb calcium from food that protein, which forms the reasonable high dairy products of goat milk calcium content, and calcium can promote human body bone
Bone growth and development promotes teenager's bone growth and prevention senile osteoporosis, goat milk calcic 1200mg-1400mg/L, ox
Milk is 800mg/L.(5) protein, which forms reasonable goat milk and is rich in bioactive substance goat milk, contains various bioactivators,
Epithelial cell growth factor (EGF) such as identical with people's milk plays a significant role maintenance alimentary canal health and skin, goat milk
The nucleotide also containing there are many, cyclic adenosine monophosphate (cAMP) (cAMP) content is 2 times of milk, can promote the digestibility and utilization of lactose；Goat milk
In also contain insulin-like growth factor, to old-aged diabetes tool have certain effect, here it is Compendium of Material Medica and " pharmacological property
By " in goat milk on the books " can control and quench one's thirst ", therefore goat milk all has good battalion to infant, pregnant woman, old man and patient
Feeding and health-care efficacy.
Probiotics (Probiotics) refers to the microorganism beneficial to human body living, when giving sufficient dosage, to host
Health it is advantageous.So far from April, 2010, the Ministry of Public Health, which successively discloses, can be used for totally 26 kinds of strain list of food.Probiotics
It is with the following functions: the effect of whole intestines adjusts microbial state treatment, prevents and treats diarrhea；Alleviate lactose intolerance symptom, promotes body nutrition
It absorbs；Prevent vagina infection；Metabolite generates biological antagonist, enhances human immunity；Reduce allergy effect；Reduce serum gallbladder
Sterol；Pre- anti-cancer and inhibition tumour growth etc.；Treatment and alleviation diarrhea etc..The probiotic products of domestic-developed are mainly at present
The report of the products such as probiotics preparation and acidified milk and milk beverage, probiotics goat milk piece technology of preparing is few.
Summary of the invention
The purpose of the present invention is to provide a kind of probiotics goat milk pieces and preparation method thereof.
In order to achieve the above objectives, probiotics goat milk piece of the invention include: by mass percentage include 85.9%~
90.995% full-cream goat milk powder, the probiotics freeze-dried vaccine powder of 0.005%-0.1%, the dietary fiber of 4%-6%, 5%-8%
Functional sweetener.
The probiotics freeze-dried vaccine powder is the mixture of probiotics and compound freeze drying protectant；
The wherein ratio of the compound freeze drying protectant of probiotics bacterial powder and the prebiotic bacterium mud of the every g of compound freeze drying protectant and 1-3g
Mixing；
Wherein probiotics is lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum, bifidobacterium breve, two
Discrimination Bifidobacterium, bifidobacterium lactis, animal bifidobacteria one or more arbitrary proportions Mixed Microbes；
The compound every 100g composition of freeze drying protectant are as follows: desalted whey powder 12-20g, date polysaccharide 3-5g, whey protein concentrate
5-11g, phosphate buffer 20-30g, distilled water 34-60g
The functional sweetener includes one in xylitol, antierythrite, lactitol, hydroxyl isomaltulose or Tagatose
Kind is a variety of
Preparation method of the invention the following steps are included:
1) prepared by probiotics freeze-dried vaccine powder:
The glucose of 1-2%, the casein hydrolysis of 0.2-0.4% is added into Semen Lablab Album extracting solution by mass volume ratio
Object, the sodium acetate of 0.1-0.3%, 0.2-0.4% diammonium hydrogen citrate, the date polysaccharide of 0.05-0.10% and 0.05%
Cysteine hydrochloride obtains culture medium, and nitrogen is filled with into culture medium, then connects respectively into culture medium by the volume ratio of 2-5%
Enter the probiotics activated, in 37 DEG C of constant temperature incubation 18-24h, refrigerated centrifuge is collected probiotics bacterium mud, added into probiotics bacterium mud
The compound freeze drying protectant for adding 1-3 times of bacterium mud weight is placed in 8-15 DEG C of cold treatment 1-2h, is lyophilized using vacuum freeze drier
For 20-24h to get freeze-drying survival rate in 85-96%, viable count is 1.2 × 1011CFU/g-5.5×1011The probiotics of CFU/g is frozen
Dry bacterium powder；
2) it is prepared containing dietary fiber and functional sweetener goat milk powder
By raw material sheep cream is purified and standardization, then it is concentrated under reduced pressure, solid content is made to reach 45-52%, and
It is uniformly mixing to obtain full-cream sheep cream, takes 85.9%~90.995% full-cream goat milk powder, 0.005%-0.1% by mass percentage
Probiotics freeze-dried vaccine powder, the dietary fiber of 4%-6% and the functional sweetener of 5%-8%, by full-cream goat milk powder, diet is fine
Peacekeeping functional sweetener is uniformly mixed, then obtains the goat milk powder containing dietary fiber and functional sweetener by spray drying
3) prepared by probiotics goat milk piece
By the full-cream goat milk powder containing dietary fiber and functional sweetener under low temperature, aseptic condition with probiotics freeze-dried vaccine
Powder is uniformly mixed, and probiotics goat milk piece then is made in probiotics goat milk powder using tablet press machine, number of live bacteria of probiotics reaches 2.5
×106-3.6×108CFU/g。
The refrigerated centrifuge condition of the step 1) be 6000-8000rpm, 10-20min, 5-10 DEG C.
The compound freeze drying protectant of the step 1) every 100g 12-20g containing desalted whey powder, date polysaccharide 3-5g, concentration
Lactalbumin 5-11g, phosphate buffer 20-30g, distilled water 34-60g.
The Semen Lablab Album extracting solution of the step 1) is prepared as follows: take Semen Lablab Album crushing to make its granularity 20-60 mesh,
It soaks in water to be added after 20-30min by 1:15 mass ratio again and boil, and maintain boiling 30min, then with 400 mesh filter-cloth filterings, obtain
Filtrate is obtained, distilled water heating is added by the mass ratio of 1:10 again in filter residue and boils and maintain 20min, is then filtered, is closed
And filtrate obtains Semen Lablab Album extracting solution twice.
The date polysaccharide of the step 1) is prepared as follows: carrying out being beaten to obtain jujube slurry after taking fresh Chinese dates to clean, press
Mass volume ratio to jujube starch in be added 0.2% pectase, 0.1% cellulase and 0.1% hemicellulase, in 60 DEG C
2-3h is kept the temperature, then filters and obtains Chinese date juice, access the wine brewing ferment of 4-8% activated after being cooled to 38-40 DEG C by volume
Mother terminates culture after 30 DEG C of constant temperature incubation 28-36h, the supernatant after saccharomyces cerevisiae medium centrifugal is concentrated into 1/3 through low temperature
The dehydrated alcohol of 3 times of volumes is added afterwards, date polysaccharide can be obtained by standing 3h suction filtration.
The present invention is added to proliferation factor, the anti-freezing factor of the integration of drinking and medicinal herbs date polysaccharide as probiotics in the medium
And freeze drying protectant, the proliferation and its frost resistance of probiotics are promoted, then it is subjected to cold treatment at low temperature, induces generation
Antifreeze protein further increases its frost resistance, then reduces cryohydric point by compound freeze drying protectant and increase to probiotics
Protective effect, to obtain high-activity probiotics bacterium powder.The number of live bacteria of probiotics added in probiotics goat milk piece of the invention
Height can be improved consumer's beneficial bacteria of intestinal tract quantity and adjust colony balance, and the dietary fiber glucan and xylo-oligosaccharide of addition can
Promote the proliferation of beneficial bacteria of intestinal tract and the absorption of minerals and increases intestines peristalsis, prevent constipation, the functional sweet taste of addition
Agent have it is low in calories, not will lead to saprodontia and obesity, on blood glucose also without influence, and there is relax bowel and defecation and promote calcium uptake etc.
Function, therefore probiotics goat milk piece can not only be used for public all suitable functional food, be alternatively arranged as anti-caries food and diabetes
Food.
Specific embodiment
Embodiment 1
Probiotics goat milk piece of the invention include: by mass percentage include 90.995% full-cream goat milk powder, 0.005%
Lactobacillus fermenti freeze-dried vaccine powder, 4% glucan, 2.5% xylitol and 2.5% Tagatose.
The preparation of probiotics goat milk piece the following steps are included:
First: the preparation of lactobacillus fermenti bacterium powder glucose, casein hydrolysate, sodium acetate, lemon in Semen Lablab Album extracting solution
Lemon acid hydrogen diammonium and date polysaccharide, making its additive amount (w/v) is respectively 1%, 0.2%, 0.1%, 0.2% and 0.05%, then
The lactobacillus fermenti activated is accessed by the inoculum concentration of 2% (v/v), terminates culture, refrigerated centrifuge in 37 DEG C of constant temperature incubation 18h
(6000rpm, 10min, 5 DEG C) obtains lactobacillus fermenti bacterium mud, and it is (every then to add 1 times of bacterium mud weight of compound freeze drying protectant
100g composition are as follows: desalted whey powder 12g, date polysaccharide 3g, whey protein concentrate 5g, phosphate buffer 20g, distilled water 60g),
8 DEG C of cold treatment 2h are subsequently placed in, are then frozen using vacuum freeze drier freeze-drying 22h to get lactobacillus fermenti freeze-dried vaccine powder
For dry survival rate 86%, viable count is 1.8 × 1011CFU/g。
Second: the preparation of dietary fiber goat milk powder is purified by raw material sheep cream and standardizes, and is then concentrated under reduced pressure, removes big portion
Point water, so that solid content is reached 48%, and stir evenly, obtain being concentrated full-cream sheep cream, desalted whey powder, dense is then added
Contracting lactalbumin and glucan, then full-cream dietary fiber goat milk powder is obtained by spray drying.
Third: the preparation of probiotics goat milk piece is by the full-cream goat milk powder containing dietary fiber and functional sweetener in low temperature, nothing
It is mixed with lactobacillus fermenti bacterium powder by equal increments under the conditions of bacterium, probiotics bacterial powder additive amount is 0.005%, then passes through tabletting
Probiotics goat milk piece is obtained, number of live bacteria of probiotics reaches 2.5 × 106CFU/g。
Embodiment 2
Probiotics goat milk piece of the invention includes: the full-cream goat milk powder for by mass percentage including 87.97%, and 0.03%
Lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum composite bacterium powder, 6% xylo-oligosaccharide, 3% red moss
Sugar alcohol, 3% lactitol.
The preparation of probiotics goat milk piece the following steps are included:
First: the preparation of probiotic lactobacilli bacterium powder glucose, casein hydrolysate, sodium acetate, lemon in Semen Lablab Album extracting solution
Lemon acid hydrogen diammonium and date polysaccharide, making its additive amount (w/v) is respectively 2%, 0.4%, 0.3%, 0.4% and 0.08%, then
The lactobacillus fermenti activated, lactobacillus acidophilus, Lactobacillus casei, plant cream bar are respectively connected to by the inoculum concentration of 5% (v/v)
Bacterium, lactobacillus bulgaricus and Roy's formula lactobacillus are terminated in 37 DEG C of constant temperature incubation 20h and are cultivated, refrigerated centrifuge (7000rpm,
15min, 10 DEG C) probiotic lactobacilli bacterium mud is obtained, then add 2 times of bacterium mud weight of compound freeze drying protectant (every 100g composition
Are as follows: desalted whey powder 12g, date polysaccharide 4g, whey protein concentrate 6g, phosphate buffer 25g, distilled water 53g), it is subsequently placed in
10 DEG C of cold treatment 1.5h, then using vacuum freeze drier freeze-drying for 24 hours to get lactobacillus fermenti, lactobacillus acidophilus, cheese cream
Bacillus, lactobacillus plantarum, lactobacillus bulgaricus and Roy's formula lactobacillus composite bacterium powder, freeze-drying survival rate is living 90%
Bacterium number is 2.6 × 1011CFU/g。
Second: the preparation of dietary fiber goat milk powder is purified by raw material sheep cream and standardizes, and is then concentrated under reduced pressure, is removed
Most water makes solid content reach 47%, and stirs evenly, and obtains concentration and takes off full-cream sheep cream, desalination cream is then added
Clear powder, whey protein concentrate, glucan and xylo-oligosaccharide.Dietary fiber goat milk powder is obtained by spray drying again.
Third: the preparation of probiotics goat milk piece is by the full-cream goat milk powder containing dietary fiber and functional sweetener in low temperature, nothing
It is mixed by equal increments with the composite bacterium powder of lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum under the conditions of bacterium
It closes, probiotics bacterial powder additive amount is 0.03%, then obtains probiotics goat milk piece by tabletting, and number of live bacteria of probiotics reaches
6.24×107CFU/g。
Embodiment 3
A kind of probiotics goat milk powder, by mass fraction containing 655 parts of goat milk powder, 250 parts of desalted whey powder, 50 parts low
The compound bacteria of xylan, 44.8 parts of whey protein concentrate and 0.2 part of bifidobacterium breve, bifidobacterium lactis, animal bifidobacteria
Powder.
Probiotics goat milk piece of the invention includes: the full-cream goat milk powder for by mass percentage including 88.98%, and 0.02%
Bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, animal bifidobacteria composite bacterium powder, 2% glucan, 3% it is low
Xylan, 6% oligoisomaltose.
The preparation of probiotics goat milk piece the following steps are included:
First: the preparation of probiotics bacterial powder glucose, casein hydrolysate, sodium acetate, citric acid in Semen Lablab Album extracting solution
Hydrogen diammonium, date polysaccharide and cysteine hydrochloride, make its additive amount (w/v) be respectively 1.0%, 0.3%, 0.2%, 0.4%,
0.10% and 0.05%, then by 2% (v/v) inoculum concentration access bifidobacterium breve, two qi Bifidobacteriums, bifidobacterium lactis,
Animal bifidobacteria terminates culture in 37 DEG C of constant temperature incubation 124h, and it is double that refrigerated centrifuge (8000rpm, 16min, 8 DEG C) collects acquisition
Discrimination bacillus bacterium mud, then add 3 times of bacterium mud weight compound freeze drying protectant (every 100g composition are as follows: desalted whey powder 18g, greatly
Jujube polysaccharide 3.5g, whey protein concentrate 8.5g, phosphate buffer 27g, distilled water 43g), 12 DEG C of cold treatment 1.4h are subsequently placed in,
Then using vacuum freeze drier freeze-drying 22h to get bifidobacterium breve, bifidobacterium lactis, animal bifidobacteria composite bacterium powder,
Survival rate is lyophilized 92% in it, and viable count is 3.8 × 1011CFU/g。
Second: the preparation of dietary fiber goat milk base is purified by raw material sheep cream and standardizes, and is then concentrated under reduced pressure, removes big portion
Point water, so that solid content is reached 50%, and stir evenly, obtain being concentrated full-cream sheep cream, desalted whey powder, dense is then added
Contracting lactalbumin and xylo-oligosaccharide, then the full-cream goat milk powder of dietary fiber is obtained by spray drying.
Third: the preparation of probiotics goat milk piece is by the full-cream goat milk powder containing dietary fiber and functional sweetener in low temperature, nothing
It is mixed by equal increments with the composite bacterium powder of lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum under the conditions of bacterium
It closes, composite bacterium powder additive amount is 0.02%, then obtains probiotics goat milk piece by tabletting, and number of live bacteria of probiotics reaches 5.72
×107CFU/g。
Embodiment 4
A kind of probiotics goat milk powder contains 709 parts of goat milk powder, 200 parts of desalted whey powder, 30 parts of Portugal by mass fraction
Glycan, 25 parts of xylo-oligosaccharide, 35 parts of whey protein concentrate and 1 part of lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei,
Lactobacillus plantarum, lactobacillus bulgaricus, bifidobacterium breve, bifidobacterium lactis, animal bifidobacteria, Roy formula lactobacillus are answered
Combined bacteria powder.
Probiotics goat milk piece of the invention includes: the full-cream goat milk powder for by mass percentage including 85.9%, 0.1% benefit
Raw bacterium freeze-dried vaccine powder, 3% glucan, 3% xylo-oligosaccharide, 2% xylitol, 1% lactitol, 1.5% oligomeric different wheat
Bud sugar alcohol, 1.5% antierythrite and 2% Tagatose.
The preparation of probiotics goat milk piece the following steps are included:
First: the preparation of probiotics bacterial powder glucose, casein hydrolysate, sodium acetate, citric acid in Semen Lablab Album extracting solution
Hydrogen diammonium and date polysaccharide, making its additive amount (w/v) is respectively 1.5%, 0.25%, 0.15%, 0.35% and 0.10%, is being trained
It need to be filled with nitrogen when supporting Bifidobacterium, and add 0.05% cysteine hydrochloride, then press the inoculum concentration of 3.2% (v/v)
Access lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum, lactobacillus bulgaricus, the short bifid activated
The probiotics such as bacillus, bifidobacterium lactis, animal bifidobacteria, Roy's formula lactobacillus terminate culture in 37 DEG C of constant temperature incubations for 24 hours,
Refrigerated centrifuge (7500rpm, 16min, 10 DEG C), which is collected, obtains probiotics bacterium mud, then adds 1.5 times of bacterium mud weight of compound jelly
Dry protective agent (every 100g composition are as follows: desalted whey powder 18g, date polysaccharide 5g, whey protein concentrate 10g, phosphate buffer 23g,
Distilled water 44g), be subsequently placed in 10 DEG C of cold treatment 2h, then using vacuum freeze drier freeze-drying for 24 hours to get lactobacillus fermenti,
Lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum, lactobacillus bulgaricus, bifidobacterium breve, bifidobacterium lactis, animal are double
Discrimination bacillus, Roy's formula lactobacillus composite bacterium powder, for freeze-drying survival rate 92%, viable count is 4.8 × 1011CFU/g。
Second: the preparation of dietary fiber goat milk base is purified by raw material sheep cream and standardizes, and is then concentrated under reduced pressure, removes big portion
Point water, so that solid content is reached 52%, and stir evenly, obtain being concentrated full-cream sheep cream, desalted whey powder, dense is then added
Contracting lactalbumin, glucan and xylo-oligosaccharide, then the full-cream goat milk powder of dietary fiber is obtained by spray drying.
Third: the preparation of probiotics goat milk piece is by the full-cream goat milk powder containing dietary fiber and functional sweetener in low temperature, nothing
It is mixed by equal increments with the composite bacterium powder of lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum under the conditions of bacterium
It closes, probiotics bacterial powder additive amount is 0.1%, then obtains probiotics goat milk piece by tabletting, and number of live bacteria of probiotics reaches 3.6
×108CFU/g。
Claims (9)
1. a kind of probiotics goat milk piece, it is characterised in that: by mass percentage include 85.9%~90.995% full-cream goat milk
Powder, the probiotics freeze-dried vaccine powder of 0.005%-0.1%, the dietary fiber of 4%-6% and the functional sweetener of 5%-8%.
2. probiotics goat milk piece according to claim 1, it is characterised in that: the probiotics freeze-dried vaccine powder is probiotics
With the mixture of compound freeze drying protectant, wherein probiotics bacterial powder and the prebiotic bacterium mud of the every g of compound freeze drying protectant and 1-3g are answered
Close the ratio mixing of freeze drying protectant；
Wherein probiotics is lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum, bifidobacterium breve, not tally pair
Discrimination bacillus, bifidobacterium lactis, animal bifidobacteria one or more arbitrary proportions Mixed Microbes；
The compound every 100g composition of freeze drying protectant are as follows: desalted whey powder 12-20g, date polysaccharide 3-5g, whey protein concentrate 5-
11g, phosphate buffer 20-30g, distilled water 34-60g.
3. probiotics goat milk piece according to claim 1, it is characterised in that: the functional sweetener includes xylose
One of alcohol, antierythrite, lactitol, hydroxyl isomaltulose or Tagatose are a variety of.
4. probiotics goat milk piece according to claim 1, it is characterised in that: the dietary fiber is glucan, oligomeric
One or both of xylose.
5. a kind of preparation method of probiotics goat milk piece, it is characterised in that the following steps are included:
1) prepared by probiotics freeze-dried vaccine powder:
Be added by mass volume ratio into Semen Lablab Album extracting solution the glucose of 1-2%, the casein hydrolysate of 0.2-0.4%,
The diammonium hydrogen citrate of the sodium acetate of 0.1-0.3%, 0.2-0.4%, the date polysaccharide of 0.05-0.10% and 0.05% half Guang
Propylhomoserin hydrochloride obtains culture medium, and nitrogen is filled with into culture medium, then accesses into culture medium respectively by the volume ratio of 2-5%
The probiotics of activation, in 37 DEG C of constant temperature incubation 18-24h, refrigerated centrifuge collects probiotics bacterium mud, adds bacterium into probiotics bacterium mud
The compound freeze drying protectant of 1-3 times of mud weight is placed in 8-15 DEG C of cold treatment 1-2h, and 20- is lyophilized using vacuum freeze drier
For 24 hours to get freeze-drying survival rate in 85-96%, viable count is 1.2 × 1011CFU/g-5.5×1011The probiotics of CFU/g is lyophilized
Bacterium powder；
2) it is prepared containing dietary fiber and functional sweetener goat milk powder
By raw material sheep cream is purified and standardization, then it is concentrated under reduced pressure, so that solid content is reached 45-52%, and stir
Full-cream sheep cream is uniformly obtained, takes 85.9%~90.995% full-cream goat milk powder, the benefit of 0.005%-0.1% by mass percentage
Raw bacterium freeze-dried vaccine powder, the dietary fiber of 4%-6% and the functional sweetener of 5%-8%, by full-cream goat milk powder, dietary fiber and
Functional sweetener is uniformly mixed, then obtains the goat milk powder containing dietary fiber and functional sweetener by spray drying
3) prepared by probiotics goat milk piece
Full-cream goat milk powder containing dietary fiber and functional sweetener is mixed under low temperature, aseptic condition with probiotics freeze-dried vaccine powder
It closing uniformly, probiotics goat milk powder is then made probiotics goat milk piece using tablet press machine, number of live bacteria of probiotics reaches 2.5 ×
106-3.6×108CFU/g。
6. the preparation method of probiotics goat milk piece according to claim 5, it is characterised in that: the freezing of the step 1) from
Heart condition be 6000-8000rpm, 10-20min, 5-10 DEG C.
7. the preparation method of probiotics goat milk piece according to claim 5, it is characterised in that: the compound jelly of the step 1)
The dry every 100g 12-20g containing desalted whey powder of protective agent, date polysaccharide 3-5g, whey protein concentrate 5-11g, phosphate buffer 20-
30g, distilled water 34-60g.
8. the preparation method of probiotics goat milk piece according to claim 5, it is characterised in that: the Semen Lablab Album of the step 1)
Extracting solution is prepared as follows: taking Semen Lablab Album crushing to make its granularity 20-60 mesh, then soaks in water 20- by 1:15 mass ratio
It is added and boils after 30min, and maintain boiling 30min, then use 400 mesh filter-cloth filterings, obtain filtrate, press 1:10 again in filter residue
Mass ratio is added distilled water heating and boils and maintain 20min, is then filtered, and merges filtrate twice and obtains Semen Lablab Album extracting solution.
9. the preparation method of probiotics goat milk piece according to claim 5, it is characterised in that: the jujube of the step 1)
Polysaccharide is prepared as follows: carrying out being beaten to obtain jujube slurry after taking fresh Chinese dates to clean, be added by mass volume ratio into jujube slurry
Then 0.2% pectase, 0.1% cellulase and 0.1% hemicellulase are filtered and are obtained in 60 DEG C of heat preservation 2-3h
Chinese date juice accesses the saccharomyces cerevisiae of 4-8% activated, 30 DEG C of constant temperature incubation 28-36h by volume after being cooled to 38-40 DEG C
Culture is terminated afterwards, and the supernatant after saccharomyces cerevisiae medium centrifugal is added to the anhydrous second of 3 times of volumes after low temperature is concentrated into 1/3
Alcohol, date polysaccharide can be obtained by standing 3h suction filtration.
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Application publication date: 20181207 |