CN108935704A - A kind of probiotics goat milk powder and preparation method thereof - Google Patents
A kind of probiotics goat milk powder and preparation method thereof Download PDFInfo
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- CN108935704A CN108935704A CN201810779982.7A CN201810779982A CN108935704A CN 108935704 A CN108935704 A CN 108935704A CN 201810779982 A CN201810779982 A CN 201810779982A CN 108935704 A CN108935704 A CN 108935704A
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- probiotics
- goat milk
- milk powder
- powder
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- 238000002360 preparation method Methods 0.000 title claims abstract description 27
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/16—Agglomerating or granulating milk powder; Making instant milk powder; Products obtained thereby
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/156—Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/143—Fermentum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
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- A23V2400/515—Animalis
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/517—Bifidum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/519—Breve
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A kind of probiotics goat milk powder and preparation method thereof; the present invention is added to proliferation factor, the anti-freezing factor and freeze drying protectant of the integration of drinking and medicinal herbs date polysaccharide as probiotics in the medium; promote the proliferation and its frost resistance of probiotics; it is subjected to cold treatment at low temperature again; the antifreeze protein that induction generates further increases its frost resistance; cryohydric point is reduced by compound freeze drying protectant again and increases the protective effect to probiotics, to obtain high-activity probiotics bacterium powder.The desalted whey powder of addition in probiotics goat milk powder of the invention and whey protein concentrate absorption easy to digest, the number of live bacteria of probiotics of addition is high, consumer's beneficial bacteria of intestinal tract quantity can be improved and adjust colony balance, the dietary fiber glucan and xylo-oligosaccharide of addition can promote the proliferation of beneficial bacteria of intestinal tract and the absorption of minerals and increase intestines peristalsis, prevent constipation, to be beneficial to consumer health.
Description
Technical field
The present invention relates to a kind of functional goat milk powders and preparation method thereof, and in particular to one kind contains probiotics
(Probiotics) and the probiotics goat milk powder of dietary fiber and preparation method thereof.
Background technique
Goat milk has following nutritive peculiarity: (1) protein, which forms protein in reasonable goat milk, mainly casein and whey
Albumen composition, the ratio between goat milk, milk, the casein in people's milk and lactalbumin are about are as follows: 75:25,80:20,60:40, group
At closer to breast milk, because casein content is relatively low in sheep cream, formation curds mass is small and relatively soft under one's belt, suction easy to digest
It receives, so the digestibility of sheep milk protein is higher than milk, is more suitable for baby and old man drinks.(2) protein forms reasonable goat milk
Protein is not easy the α in allergy milkS1Casein and beta lactoglobulin are anaphylactogens generally acknowledged at present, can be caused allergic reaction, out
The adverse reactions such as existing fash, eczema and abdominal pain diarrhea.α in milkS1Casein accounts for the 43% of casein, and 1- is only accounted in goat milk
3%, therefore drink goat milk and be not easy allergy (3) protein and form the reasonable goat milk fat fat globule easy to digest absorbed in goat milk
It is 3.49 μm, cow's milk fat globule is 4.55 μm, since the fatty bulb diameter of goat milk is small, is easy to be digested, digestibility is up to 97-
98%, while the fatty acid in goat milk is not easy to accumulate in human body based on middle chain and short chain, one, U.S. scientist studies table
Bright, goat milk fat is mainly consumed in the form of heat in body metabolism, the interior accumulation of human body will not generate puffiness phenomenon again.(4)
It is the main source that people absorb calcium from food that protein, which forms the reasonable high dairy products of goat milk calcium content, and calcium can promote human body bone
Bone growth and development promotes teenager's bone growth and prevention senile osteoporosis, goat milk calcic 1200mg-1400mg/L, ox
Milk is 800mg/L.(5) protein, which forms reasonable goat milk and is rich in bioactive substance goat milk, contains various bioactivators,
Epithelial cell growth factor (EGF) such as identical with people's milk plays a significant role maintenance alimentary canal health and skin, goat milk
The nucleotide also containing there are many, cyclic adenosine monophosphate (cAMP) (cAMP) content is 2 times of milk, can promote the digestibility and utilization of lactose；Goat milk
In also contain insulin-like growth factor, to old-aged diabetes tool have certain effect, here it is Compendium of Material Medica and " pharmacological property
By " in goat milk on the books " can control and quench one's thirst ", therefore goat milk all has good battalion to infant, pregnant woman, old man and patient
Feeding and health-care efficacy.
Probiotics (Probiotics) refers to the microorganism beneficial to human body living, when giving sufficient dosage, to host
Health it is advantageous.So far from April, 2010, the Ministry of Public Health, which successively discloses, can be used for totally 26 kinds of strain list of food.Probiotics
It is with the following functions: the effect of whole intestines adjusts microbial state treatment, prevents and treats diarrhea；Alleviate lactose intolerance symptom, promotes body nutrition
It absorbs；Prevent vagina infection；Metabolite generates biological antagonist, enhances human immunity；Reduce allergy effect；Reduce serum gallbladder
Sterol；Pre- anti-cancer and inhibition tumour growth etc.；Treatment and alleviation diarrhea etc..
China develops some probiotics preparations and probiotic bacteria milk product, probiotics preparation such as Infantile diarrhea (long bifid at present
Bacillus, lactobacillus bulgaricus and streptococcus thermophilus), LIVZON CHANGLE (Bifidobacterium) and Red cell function (bifidobacterium longum, acidophilus
Lactobacillus and streptococcus fecalis), dairy products are such as " the hat benefit cream " of Mongolia Ox, bright " AB100 ", " beneficial bacteria is more " of ternary, Erie
" light fruit is inspired ", " the increasingly bacterium yoghurt " of Long Dan etc., compared with foreign countries, probiotic bacteria milk product is mainly acidified milk or milk beverage
Equal liquid diary products, the milk powder product of probiotics are seldom.Patent (CN 100506055C) disclose a kind of red date sheep milk powder and its
Preparation method, the substance of addition are integration of drinking and medicinal herbs substance jujube.(horsepower, Han Xuelin, Xu Wei Rong's old age goat milk powders such as horsepower
Development Food Science, 1993 (1): 26-30) report the development of old goat milk powder a kind of.
Summary of the invention
The purpose of the present invention is to provide a kind of probiotics goat milk powders and preparation method thereof.
To achieve the goals above, preparation method of the invention, it is characterised in that the following steps are included:
1) prepared by probiotics bacterial powder:
The glucose of 1-2%, the casein hydrolysis of 0.2-0.4% is added into Semen Lablab Album extracting solution by mass volume ratio
Object, the sodium acetate of 0.1-0.3%, 0.2-0.4% diammonium hydrogen citrate, the date polysaccharide of 0.05-0.10% obtains culture medium, to
It is filled with nitrogen in culture medium, then accesses the probiotics activated into culture medium by the volume ratio of 2-5%, is trained in 37 DEG C of constant temperature
18-24h is supported, refrigerated centrifuge collects probiotics bacterium mud, and the compound frozen-dried protective of 1-3 times of bacterium mud weight is added into probiotics bacterium mud
Agent is placed in 8-15 DEG C of cold treatment 1-2h, using vacuum freeze drier freeze-drying 20-24h to get freeze-drying survival rate in 85-
96%, viable count is 1.2 × 1011CFU/g-5.5×1011The probiotics bacterial powder of CFU/g；
2) prepared by dietary fiber goat milk powder
By mass fraction take 589-730 parts of degreasing goat milk powder or full-cream goat milk powder, 200-300 parts of desalted whey powder,
40-60 parts of dietary fibers and 30-50 parts of whey protein concentrate are uniformly mixed, then by spray drying obtain dietary fiber degreasing or
Full-cream goat milk powder；
3) prepared by probiotics goat milk powder
Dietary fiber degreasing or full-cream goat milk powder are added 0.03-1.0 parts by mass fraction under low temperature, aseptic condition
Probiotics bacterial powder makes the final viable count in probiotics goat milk powder reach 3.6 × 106-5.2×108cfu/g。
The refrigerated centrifuge condition of the step 1) be 6000-8000rpm, 10-20min, 5-10 DEG C.
The compound freeze drying protectant of the step 1) every 100g 12-20g containing desalted whey powder, date polysaccharide 3-5g, concentration
Lactalbumin 5-11g, phosphate buffer 20-30g, distilled water 34-60g.
The Semen Lablab Album extracting solution of the step 1) is prepared as follows: take Semen Lablab Album crushing to make its granularity 20-60 mesh,
It soaks in water to be added after 20-30min by 1:15 mass ratio again and boil, and maintain boiling 30min, then with 400 mesh filter-cloth filterings, obtain
Filtrate is obtained, distilled water heating is added by the mass ratio of 1:10 again in filter residue and boils and maintain 20min, is then filtered, is closed
And filtrate obtains Semen Lablab Album extracting solution twice.
The date polysaccharide of the step 1) is prepared as follows: carrying out being beaten to obtain jujube slurry after taking fresh Chinese dates to clean, press
Mass volume ratio to jujube starch in be added 0.2% pectase, 0.1% cellulase and 0.1% hemicellulase, in 60 DEG C
2-3h is kept the temperature, then filters and obtains Chinese date juice, access the wine brewing ferment of 4-8% activated after being cooled to 38-40 DEG C by volume
Mother terminates culture after 30 DEG C of constant temperature incubation 28-36h, the supernatant after saccharomyces cerevisiae medium centrifugal is concentrated into 1/3 through low temperature
The dehydrated alcohol of 3 times of volumes is added afterwards, date polysaccharide can be obtained by standing 3h suction filtration.
The probiotics uses lactobacillus, Bifidobacterium, needs to be added in step 1) using Bifidobacterium and cultivate
The cysteine hydrochloride of culture medium quality 0.05% is added in base.
The lactobacillus is lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum；
The Bifidobacterium is bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, animal bifidobacteria.
The dietary fiber is one or both of glucan, xylo-oligosaccharide.
By probiotics goat milk powder made of preparation method of the invention, by mass fraction containing 589-730 parts goat milk powder,
200-300 parts of desalted whey powder, 40-60 parts of dietary fiber, 30-50 parts of whey protein concentrate and 0.03-1 parts it is prebiotic
Bacterium.
The present invention is added to proliferation factor, the anti-freezing factor of the integration of drinking and medicinal herbs date polysaccharide as probiotics in the medium
And freeze drying protectant, the proliferation and its frost resistance of probiotics are promoted, then it is subjected to cold treatment at low temperature, induces generation
Antifreeze protein further increases its frost resistance, then reduces cryohydric point by compound freeze drying protectant and increase to probiotics
Protective effect, to obtain high-activity probiotics bacterium powder.The desalted whey powder of addition in probiotics goat milk powder of the invention
It is high with the number of live bacteria of probiotics of whey protein concentrate absorption easy to digest, addition, consumer's beneficial bacteria of intestinal tract quantity can be improved simultaneously
Colony balance is adjusted, the dietary fiber glucan and xylo-oligosaccharide of addition can promote the proliferation of beneficial bacteria of intestinal tract and the suction of minerals
Receive and increase intestines peristalsis, prevent constipation, to be beneficial to consumer health.
Specific embodiment
Embodiment 1:
1) prepared by probiotics bacterial powder:
Glucose, casein hydrolysate, sodium acetate, diammonium hydrogen citrate and date polysaccharide, make in Semen Lablab Album extracting solution
Its additive amount (w/v) is respectively 1%, 0.2%, 0.1%, 0.2% and 0.05%, then by the inoculum concentration access of 2% (v/v)
The lactobacillus fermenti of activation terminates culture in 37 DEG C of constant temperature incubation 18h, and refrigerated centrifuge (6000rpm, 10min, 5 DEG C) is sent out
Kefir milk bacillus bacterium mud, then add 1 times of bacterium mud weight compound freeze drying protectant (every 100g composition are as follows: desalted whey powder 12g,
Date polysaccharide 3g, whey protein concentrate 5g, phosphate buffer 20g, distilled water 60g), 8 DEG C of cold treatment 2h are subsequently placed in, are then adopted
With vacuum freeze drier freeze-drying 22h to get lactobacillus fermenti freeze-dried vaccine powder, survival rate is lyophilized 86%, viable count 1.8
×1011CFU/g。
2) prepared by dietary fiber goat milk powder:
It is raw material sheep cream is purified and standardization is if it is the preparation full-cream goat milk powder of probiotics can be directly entered next
A step then needs separation of cream, need to heat carry out degreasing, fat content is controlled if it is preparation probiotics degreasing goat milk powder
< 0.2%.Full-cream sheep cream obtained above or degreasing sheep cream are concentrated under reduced pressure, most water is removed, contains solid content
Amount reaches 48%, and stirs evenly, and obtains condensed skimmed sheep cream or full-cream sheep cream, desalted whey powder, condensed whey is then added
Albumen and glucan, then dietary fiber degreasing or full-cream goat milk powder are obtained by spray drying.
3) prepared by probiotics goat milk powder
Dietary fiber degreasing or full-cream goat milk powder are mixed under low temperature, aseptic condition with lactobacillus fermenti freeze-dried vaccine powder,
Additive amount carries out equal increments mixing by 0.03g/kg, and the final viable count in probiotics goat milk powder reaches 5.4 × 106CFU/g。
By probiotics goat milk powder made from method made above, by mass fraction containing 589.97 parts goat milk powder, 300 parts
Desalted whey powder, 60 parts of glucan, 50 parts of whey protein concentrate and 0.03 part of lactobacillus fermenti bacterium powder.
Embodiment 2
1) prepared by probiotics bacterial powder:
Glucose, casein hydrolysate, sodium acetate, diammonium hydrogen citrate and date polysaccharide, make in Semen Lablab Album extracting solution
Its additive amount (w/v) is respectively 2%, 0.4%, 0.3%, 0.4% and 0.08%, is then connect respectively by the inoculum concentration of 5% (v/v)
Enter the lactobacillus fermenti activated, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum, terminates training in 37 DEG C of constant temperature incubation 20h
It supports, refrigerated centrifuge (7000rpm, 15min, 10 DEG C) obtains probiotic lactobacilli bacterium mud, then adds 2 times of bacterium mud weight of compound jelly
Dry protective agent (every 100g composition are as follows: desalted whey powder 12g, date polysaccharide 4g, whey protein concentrate 6g, phosphate buffer 25g,
Distilled water 53g), 10 DEG C of cold treatment 1.5h are subsequently placed in, are then lyophilized using vacuum freeze drier for 24 hours to get acidified milk bar
Bacterium, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum composite bacterium powder, freeze-drying survival rate is 90%, viable count 2.6
×1011CFU/g。
2) prepared by dietary fiber goat milk powder:
It is raw material sheep cream is purified and standardization is if it is the preparation full-cream goat milk powder of probiotics can be directly entered next
A step then needs separation of cream, need to heat carry out degreasing, fat content is controlled if it is preparation probiotics degreasing goat milk powder
< 0.2%.Full-cream sheep cream obtained above or degreasing sheep cream are concentrated under reduced pressure, most water is removed, contains solid content
Amount reaches 47%, and stirs evenly, and obtains condensed skimmed sheep cream or full-cream sheep cream, desalted whey powder, condensed whey is then added
Albumen, glucan and xylo-oligosaccharide, then dietary fiber degreasing or full-cream goat milk powder are obtained by spray drying.
3) prepared by probiotics goat milk powder:
By dietary fiber degreasing or full-cream goat milk powder under low temperature, aseptic condition with lactobacillus fermenti, lactobacillus acidophilus, dry
The composite bacterium powder progress equal increments mixing of Lactobacillus paracasei, lactobacillus plantarum, composite bacterium powder additive amount is 0.3g/kg, acquisition
Final viable count in probiotics goat milk powder reaches 7.8 × 107CFU/g is measured by accelerated test, which exists
4-20 DEG C of storage requirement stability inferior is good.
Goat milk powder, 200 parts de- by probiotics goat milk powder made from method made above, by mass fraction containing 730 parts
Salt whey powder, 20 parts of glucan, 20 parts of xylo-oligosaccharide, 29.7 parts of whey protein concentrate, 0.3 part of lactobacillus fermenti,
Lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum composite bacterium powder.
Embodiment 3
1) prepared by probiotics bacterial powder:
Glucose, casein hydrolysate, sodium acetate, diammonium hydrogen citrate, date polysaccharide and half in Semen Lablab Album extracting solution
Cystine hydrochloride, making its additive amount (w/v) is respectively 1.0%, 0.3%, 0.2%, 0.4%, 0.10% and 0.05%, then
Bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, animal bifidobacteria are accessed by the inoculum concentration of 2% (v/v), in 37 DEG C
Constant temperature incubation 124h terminates culture, and refrigerated centrifuge (8000rpm, 16min, 8 DEG C), which is collected, obtains Bifidobacterium bacterium mud, then adds
3 times of bacterium mud weight of compound freeze drying protectant (every 100g composition are as follows: desalted whey powder 18g, date polysaccharide 3.5g, condensed whey
Albumen 8.5g, phosphate buffer 27g, distilled water 43g), 12 DEG C of cold treatment 1.4h are subsequently placed in, vacuum freeze drying is then used
22h is lyophilized to get bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, animal bifidobacteria composite bacterium powder, freeze-drying in machine
For survival rate 92%, viable count is 3.8 × 1011CFU/g。
2) prepared by dietary fiber goat milk powder:
It is raw material sheep cream is purified and standardization is if it is the preparation full-cream goat milk powder of probiotics can be directly entered next
A step then needs separation of cream, need to heat carry out degreasing, fat content is controlled if it is preparation probiotics degreasing goat milk powder
< 0.2%.Full-cream sheep cream obtained above or degreasing sheep cream are concentrated under reduced pressure, most water is removed, contains solid content
Amount reaches 50%, and stirs evenly, and obtains condensed skimmed sheep cream or full-cream sheep cream, desalted whey powder, condensed whey is then added
Albumen and xylo-oligosaccharide, then dietary fiber degreasing or full-cream goat milk powder are obtained by spray drying.
3) prepared by probiotics goat milk powder:
By dietary fiber degreasing or full-cream goat milk powder powder under low temperature, aseptic condition with bifidobacterium breve, not tally bifid bar
Bacterium, bifidobacterium lactis, animal bifidobacteria composite bacterium powder are mixed by equal increments mode, and composite bacterium powder additive amount is 0.2g/kg,
Final viable count in probiotics goat milk powder reaches 7.6 × 107CFU/g is measured by accelerated test, which exists
4-20 DEG C of storage requirement stability inferior is good.
Goat milk powder, 250 parts de- by probiotics goat milk powder made from method made above, by mass fraction containing 655 parts
Salt whey powder, 50 parts of xylo-oligosaccharide, 44.8 parts of whey protein concentrate and 0.2 part of bifidobacterium breve, bifidobacterium bifidum, cream
Bifidobacterium, animal bifidobacteria composite bacterium powder.
Embodiment 4
1) prepared by probiotics bacterial powder:
Glucose, casein hydrolysate, sodium acetate, diammonium hydrogen citrate and date polysaccharide, make in Semen Lablab Album extracting solution
Its additive amount (w/v) is respectively 1.5%, 0.25%, 0.15%, 0.35% and 0.10%, need to be filled with when cultivating Bifidobacterium
Nitrogen, and 0.05% cysteine hydrochloride is added, the acidified milk activated is then accessed by the inoculum concentration of 3.2% (v/v)
Bacillus, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum, bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, animal
The probiotics such as Bifidobacterium terminate culture in 37 DEG C of constant temperature incubations for 24 hours, and refrigerated centrifuge (7500rpm, 16min, 10 DEG C) collection obtains
Probiotics bacterium mud is obtained, 1.5 times of bacterium mud weight of compound freeze drying protectant (every 100g composition are as follows: desalted whey powder is then added
18g, date polysaccharide 5g, whey protein concentrate 10g, phosphate buffer 23g, distilled water 44g), 10 DEG C of cold treatment 2h are subsequently placed in,
Then it is lyophilized using vacuum freeze drier for 24 hours to get lactobacillus fermenti, lactobacillus acidophilus, Lactobacillus casei, plant cream bar
Bacterium, bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, the composite bacterium powder of animal bifidobacteria, freeze-drying survival rate exists
92%, viable count is 4.8 × 1011CFU/g。
2) prepared by dietary fiber goat milk powder:
It is raw material sheep cream is purified and standardization is if it is the preparation full-cream goat milk powder of probiotics can be directly entered next
A step then needs separation of cream, need to be heated to 37 DEG C of progress degreasings, fat is contained if it is preparation probiotics degreasing goat milk powder
Amount control < 0.2%.Full-cream sheep cream obtained above or degreasing sheep cream are concentrated under reduced pressure, most water is removed, makes solid
Shape object content reaches 52%, and stirs evenly, and obtains condensed skimmed sheep cream or full-cream sheep cream, and desalted whey powder, dense is then added
Contracting lactalbumin, glucan and xylo-oligosaccharide, then dietary fiber degreasing or full-cream goat milk powder are obtained by spray drying.
3) prepared by probiotics goat milk powder:
By dietary fiber degreasing or full-cream goat milk powder under low temperature, aseptic condition with lactobacillus fermenti, lactobacillus acidophilus, dry
Lactobacillus paracasei, lactobacillus plantarum, bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, animal bifidobacteria composite bacterium powder
It is mixed in three-dimensional mixer by equal increments mode, composite bacterium powder additive amount is 1g/kg, the number of live bacteria of probiotics in goat milk powder
It is 4.8 × 108CFU/g is measured by accelerated test, and the probiotics goat milk powder is good in 4-20 DEG C of storage requirement stability inferior.
Goat milk powder, 200 parts de- by probiotics goat milk powder made from method made above, by mass fraction containing 709 parts
Salt whey powder, 30 parts of glucan, 25 parts of xylo-oligosaccharide, 35 parts of whey protein concentrate and 1 part of lactobacillus fermenti, acidophilus cream
Bacillus, Lactobacillus casei, lactobacillus plantarum, bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, animal bifidobacteria
Composite bacterium powder.
Claims (9)
1. a kind of preparation method of probiotics goat milk powder, it is characterised in that the following steps are included:
1) prepared by probiotics bacterial powder:
Be added by mass volume ratio into Semen Lablab Album extracting solution the glucose of 1-2%, the casein hydrolysate of 0.2-0.4%,
The diammonium hydrogen citrate of the sodium acetate of 0.1-0.3%, 0.2-0.4%, the date polysaccharide of 0.05-0.10% obtain culture medium, Xiang Pei
It supports in base and is filled with nitrogen, the probiotics activated is then accessed into culture medium by the volume ratio of 2-5%, in 37 DEG C of constant temperature incubations
18-24h, refrigerated centrifuge collect probiotics bacterium mud, and the compound frozen-dried protective of 1-3 times of bacterium mud weight is added into probiotics bacterium mud
Agent is placed in 8-15 DEG C of cold treatment 1-2h, using vacuum freeze drier freeze-drying 20-24h to get freeze-drying survival rate in 85-
96%, viable count is 1.2 × 1011CFU/g-5.5×1011The probiotics bacterial powder of CFU/g；
2) prepared by dietary fiber goat milk powder
589-730 parts of degreasing goat milk powder or full-cream goat milk powder, 200-300 parts of desalted whey powder, 40-60 are taken by mass fraction
Part dietary fiber and 30-50 parts of whey protein concentrate are uniformly mixed, then obtain dietary fiber degreasing or full-cream by spray drying
Goat milk powder；
3) prepared by probiotics goat milk powder
Dietary fiber degreasing or full-cream goat milk powder are pressed to the prebiotic of 0.03-1.0 parts of mass fraction addition under low temperature, aseptic condition
Bacterium bacterium powder makes the final viable count in probiotics goat milk powder reach 3.6 × 106-5.2×108cfu/g。
2. the preparation method of probiotics goat milk powder according to claim 1, it is characterised in that: the freezing of the step 1) from
Heart condition be 6000-8000rpm, 10-20min, 5-10 DEG C.
3. the preparation method of probiotics goat milk powder according to claim 1, it is characterised in that: the compound jelly of the step 1)
The dry every 100g 12-20g containing desalted whey powder of protective agent, date polysaccharide 3-5g, whey protein concentrate 5-11g, phosphate buffer 20-
30g, distilled water 34-60g.
4. the preparation method of probiotics goat milk powder according to claim 1, it is characterised in that: the Semen Lablab Album of the step 1)
Extracting solution is prepared as follows: taking Semen Lablab Album crushing to make its granularity 20-60 mesh, then soaks in water 20- by 1:15 mass ratio
It is added and boils after 30min, and maintain boiling 30min, then use 400 mesh filter-cloth filterings, obtain filtrate, press 1:10 again in filter residue
Mass ratio is added distilled water heating and boils and maintain 20min, is then filtered, and merges filtrate twice and obtains Semen Lablab Album extracting solution.
5. the preparation method of probiotics goat milk powder according to claim 1, it is characterised in that: the jujube of the step 1) is more
Sugar is prepared as follows: carrying out being beaten to obtain jujube slurry after taking fresh Chinese dates to clean, is added 0.2% into jujube slurry by mass volume ratio
Pectase, 0.1% cellulase and 0.1% hemicellulase then filter and obtain jujube in 60 DEG C of heat preservation 2-3h
Juice accesses the saccharomyces cerevisiae of 4-8% activated, end after 30 DEG C of constant temperature incubation 28-36h by volume after being cooled to 38-40 DEG C
It only cultivates, the supernatant after saccharomyces cerevisiae medium centrifugal is added to the dehydrated alcohol of 3 times of volumes after low temperature is concentrated into 1/3,
Date polysaccharide can be obtained by standing 3h suction filtration.
6. the preparation method of probiotics goat milk powder according to claim 1, it is characterised in that: the probiotics is using cream
Bacillus, Bifidobacterium need addition that culture medium quality 0.05% is added in the medium using Bifidobacterium in step 1)
Cysteine hydrochloride.
7. the preparation method of probiotics goat milk powder according to claim 1, it is characterised in that: the lactobacillus is fermentation
Lactobacillus, lactobacillus acidophilus, Lactobacillus casei, lactobacillus plantarum；
The Bifidobacterium is bifidobacterium breve, bifidobacterium bifidum, bifidobacterium lactis, animal bifidobacteria.
8. probiotics goat milk powder according to claim 1, it is characterised in that: the dietary fiber is glucan, oligomeric
One or both of xylose.
9. probiotics goat milk powder made of a kind of preparation method as described in claim 1, it is characterised in that: contain by mass fraction
589-730 parts of goat milk powder, 200-300 parts of desalted whey powder, 40-60 parts of dietary fiber, 30-50 parts of condensed whey egg
The white probiotics with 0.03-1 parts.
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| CN113396981B (en) * | 2021-06-29 | 2022-08-23 | 湖南宜贝生物科技有限公司 | Goat milk powder with stomach nourishing effect and preparation method thereof |
| CN114680184A (en) * | 2022-03-03 | 2022-07-01 | 郑州悦牛企业管理咨询有限公司 | Probiotics formula goat milk powder and production device and method thereof |
| CN116396904A (en) * | 2023-04-04 | 2023-07-07 | 陕西科技大学 | Preparation of probiotics powder and application of probiotics powder in goat milk powder |
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Application publication date: 20181207 |