CN109527099A - A kind of fructus lycii compound probiotic goat milk piece and preparation method thereof - Google Patents

A kind of fructus lycii compound probiotic goat milk piece and preparation method thereof Download PDF

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Publication number
CN109527099A
CN109527099A CN201811621622.0A CN201811621622A CN109527099A CN 109527099 A CN109527099 A CN 109527099A CN 201811621622 A CN201811621622 A CN 201811621622A CN 109527099 A CN109527099 A CN 109527099A
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probiotics
goat milk
powder
fructus lycii
preparation
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Inventor
舒国伟
毛耀龙
张乐乐
陈合
马东林
张雯
王旭
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Shaanxi University of Science and Technology
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Shaanxi University of Science and Technology
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A23C9/156—Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/18—Milk in dried and compressed or semi-solid form

Abstract

The invention discloses a kind of fructus lycii compound probiotic goat milk piece and preparation method thereof, fructus lycii compound probiotic goat milk piece includes xylitol, antierythrite, mannitol, microcrystalline cellulose, wolfberry fruit powder, goat milk powder and probiotics bacterial powder；Preparation method includes preparing probiotics bacterial powder, obtains goat milk powder by atomization drying, goat milk powder is mixed with additives such as probiotics bacterial powder, wolfberry fruit powders, then probiotics goat milk piece is made by tablet press machine；The present invention can enhance non-specific immune function, improve disease resistance, inhibit tumour growth and cell mutation and reducing blood lipid, hypoglycemic and blood pressure lowering effect；Also there is protection liver, anti-caries, the effect of insulin in liptinite is suitable for diabetic.

Description

A kind of fructus lycii compound probiotic goat milk piece and preparation method thereof
Technical field
The invention belongs to dairy products technical field, it is related to a kind of fructus lycii compound probiotic goat milk piece and preparation method thereof.
Background technique
The milk that goat secrets out of have the characteristics that it is sweet in flavor warm-natured, to the five internal organs of human body have gentle, qi-restoratives, blood-nourishing it is only Special effect.Goat milk also has the health-care effects such as antiallergy, weight-reducing.Active immune globulin present in goat milk can be improved The immunity of human body resists various diseases, furthermore also contains active factors in goat milk, can promote epithelial cell growth, rich in super Superoxide dismutase has the function of anti-inflammatory, anti-aging；DHA rich in is conducive to the intelligence and visual acuity of baby； Goat milk is compared with milk, and goat milk is in alkalescent, and milk is weakly acidic, therefore the sheep cream of meta-alkalescence has good buffering special Property, there is good rehabilitation effect for diseases such as ephritis, tuberculosis and asthma.
Probiotics is that a group is settled down in human body intestinal canal, can be generated to host after their quantity reaches a certain level beneficial The microorganism of function.There are many bacterial strains to be used as probiotics in bacterium and saccharomyces, mainly include lactobacillus, Bifidobacterium and Leuconostoc etc., wherein lactobacillus acidophilus, Bifidobacterium and Lactobacillus casei are the generally acknowledged strains with probiotic effects. Probiotics will play effect in enteron aisle, it is necessary to which to host's no pathogenicity, acid and bile salt tolerance, and after reaching enteron aisle, viable count is greater than 106/mL.For probiotics, most study is exactly lactobacillus acidophilus and Bifidobacterium at present.
Fructus lycii has various health care functions, is the dual-purpose of drug and food food of Ministry of Public Health's approval.Fructus lycii is rich in polysaccharides, Polysaccharides is a kind of water-soluble polysaccharide, by arabinose, glucose, galactolipin, mannose, xylose, rhamnose this 6 kinds of monosaccharide At being grouped as, there is physiological activity, non-specific immune function can be enhanced, improve disease resistance, inhibit tumour growth and thin Cytoplasmic process becomes and reducing blood lipid, hypoglycemic and blood pressure lowering effect.In addition, fructus lycii also has protection liver, beautifying face and moistering lotion, protection Reproductive system and the function of increasing hematopoiesis.
Wolfberry fruit powder is baked through low temperature using the natural fructus lycii in Ningxia and is cured, beaten powder and be process, fructus lycii breaks into powder not only not It will affect effect, absorption can be increased instead, promote digestion.
At present using fructus lycii as ingredient, it is mainly used in beverage industry.The patent No. (CN106387572A) discloses fructus lycii The preparation method of probiotic beverage, (CN107594261A) disclose the preparation method of honey red date fermentation probiotic beverage, when So there are also the preparation method of some probiotic tablets, the patent No. (CN106262118A) discloses the preparation of probiotics fructus lycii piece Method.But fructus lycii and probiotics connected applications are developed in goat milk product in the preparation method of tablet at present, the country is almost Do not report.Since goat milk has the advantage better than other newborn sources, fructus lycii probiotics goat milk is developed by function factor of probiotics Piece, easy to carry, it is the health food of rare men and women, old and young that nutritive value is high.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, present invention solves the technical problem that being to provide a kind of compound benefit of fructus lycii Raw bacterium goat milk piece and preparation method thereof realizes and a kind of fructus lycii and probiotics connected applications is developed tablet in goat milk product Preparation method.
The present invention is to be achieved through the following technical solutions:
A kind of fructus lycii compound probiotic goat milk piece includes: xylitol 8-10% by its mass ratio, antierythrite 2-4%, sweet Reveal sugar alcohol 3-4%, microcrystalline cellulose 0.15-0.2%, wolfberry fruit powder 10-15%, goat milk powder 65.8-76.75% and probiotics bacterial powder 0.10-1%.
A kind of preparation method of fructus lycii compound probiotic goat milk piece, preparation step are as follows:
Step 1, probiotics bacterial powder is prepared: the probiotics after access 1-5% has been activated into culture medium by volume, 37 DEG C Probiotics bacterium mud is made by centrifuge centrifugation in constant temperature incubation 20-22h；Freeze drying protectant mixing is added into probiotics bacterium mud Uniformly, -20 DEG C of cold treatment 14h-16h are placed in, probiotics bacterial powder is obtained by freeze-drying；
Step 2, the preparation of goat milk powder: sheep cream is concentrated into the concentrated milk that solid content is 42-50%, concentrated milk passes through Spray drying obtains goat milk powder；
Step 3, prepared by fructus lycii probiotics goat milk piece: it is by mass percentage xylitol 8-10%, antierythrite 2-4%, Mannitol 3-4%, microcrystalline cellulose 0.15-0.2%, wolfberry fruit powder 10-15%, goat milk powder 65.8-76.75% and probiotics bacterial Powder 0.1-1% mixing, obtains fructus lycii probiotics goat milk powder；Probiotics goat milk piece is made by tablet press machine.
Further, the probiotics accessed in step 1 includes lactobacillus acidophilus, lactobacillus plantarum and two qi Bifidobacteriums.
Further, the probiotics bacterial powder obtained in step 1 includes lactobacillus acidophilus bacterium powder, lactobacillus plantarum bacterium powder and two Qi Bifidobacteria powder, and mixed in mass ratio with the ratio of 1:1:1.
Further, the culture medium in step 1 is MRS meat soup.
Further, in step 1 probiotics bacterial powder preparation, when cultivating two qi Bifidobacteriums, after medium sterilization is cooling Also add the cysteine hydrochloride for pressing mass volume ratio 0.05%.
Further, probiotics bacterium mud is lactobacillus acidophilus bacterium mud in step 1, and mixed lactobacillus acidophilus freeze-drying is protected The mass ratio for protecting agent each component is lactose 5-10%, oligofructose 5-10%, proline 2-5%, galactolipin 5-10%, seaweed Sugared 10-15%, skimmed milk 20-40%, surplus are sterile water；Probiotics bacterium mud is lactobacillus plantarum bacterium mud, mixed plant The mass ratio of object lactobacillus freeze drying protectant each component is trehalose 10-15%, disodium hydrogen phosphate 0.2-1%, lactose 1- 5%, skimmed milk 20-40%, surplus is sterile water；Probiotics bacterium mud is two qi Bifidobacterium bacterium muds, and two mixed qis are double Discrimination bacillus freeze drying protectant each component mass ratio is glycine 2-5%, dibastic sodium phosphate 0.5-1%, xylo-oligosaccharide 5-10%, essence Propylhomoserin 2-5%, skimmed milk 20-40%, surplus are sterile water.
Further, in step 1 probiotics bacterium mud and freeze drying protectant mixed proportion are as follows: 1 gram of probiotics bacterium mud addition 1 Milliliter freeze drying protectant；Lactobacillus acidophilus freeze-drying protective agent, lactobacillus plantarum freeze drying protectant and the freeze-drying of two qi Bifidobacteriums are protected Shield agent is mixed by the volume ratio of 1:1:1.
Further, the spray drying condition of step 2 are as follows: inlet air temperature be 165 DEG C -170 DEG C, temperature of outgoing air be 65 DEG C - 70℃。
Compared with prior art, the invention has the following beneficial technical effects:
A kind of fructus lycii compound probiotic goat milk piece provided by the invention and preparation method thereof, realizes a kind of by fructus lycii and benefit Raw bacterium connected applications develop the preparation method of tablet in goat milk product；The compound probiotic added in the present invention not only can be with The immunity of body is improved, various diseases is resisted, intestinal flora, prevent constipation etc. can also be adjusted；The wolfberry fruit powder of addition has Various health care functions, can enhance non-specific immune function, improve disease resistance, inhibit tumour growth and cell mutation and Reducing blood lipid, hypoglycemic and blood pressure lowering effect；The xylitol of addition also has protection liver, prevents other than as sweetener Saprodontia, the effect of insulin in liptinite, is suitable for diabetic.
Specific embodiment
Specific embodiment is given below.
Embodiment 1
A kind of fructus lycii compound probiotic goat milk piece includes: xylitol 8.58% by its mass ratio, antierythrite 2.68%, Mannitol 3.1%, microcrystalline cellulose 0.15%, wolfberry fruit powder 10%, goat milk powder 75.39% and probiotics bacterial powder 0.10%.
A kind of preparation method of fructus lycii compound probiotic goat milk piece, preparation step are as follows:
Step 1, probiotics bacterial powder is prepared: the probiotics after access 1% has activated into culture medium by volume, 37 DEG C of perseverances Temperature culture 20h, and it is primary every 3h stirring；It is then centrifuged for machine and probiotics bacterium mud is made in 8000 turns/min centrifugation 15min centrifugation； Probiotics bacterium mud and freeze drying protectant are uniformly mixed, and are placed in -20 DEG C of cold treatment 14h, obtain probiotics bacterial powder by freeze-drying； Viable count in the bacterium powder of acquisition are as follows: lactobacillus acidophilus 1 × 1011CFU/g, bifidobacterium bifidum 1 × 1011CFU/g and plant cream Bacillus 2 × 1011The bacterium powder of CFU/g, and the ratio mixing of three 1:1:1 in mass ratio；Wherein freeze drying protectant is acidophilus cream bar Bacterium freeze drying protectant, lactobacillus plantarum freeze drying protectant and two qi Bifidobacterium freeze drying protectants are mixed by the volume ratio of 1:1:1 It forms；
Wherein, the activation process of probiotics specifically: by laboratory save lactobacillus acidophilus, bifidobacterium bifidum and Lactobacillus plantarum freeze-dried vaccine powder accesses in culture medium MRS meat soup, since bifidobacterium bifidum is strictly anaerobic, is cultivating When need to be added 0.05% cysteine hydrochloric acid in MRS meat soup and consume the oxygen in culture medium, be subsequently placed in constant temperature In 37 DEG C of culture 20h in incubator, a small amount of bacteria suspension is taken to carry out microscopy, after determining without living contaminants, presses 5% with asepsis injector Inoculum concentration access MRS meat soup in activation to 3 generations, be put into stored refrigerated spare in refrigerator.
In addition, the mixed proportion of probiotics bacterium mud and freeze drying protectant: 1 gram of probiotics bacterium mud adds 1 milliliter of frozen-dried protective Agent；Probiotics bacterium mud is lactobacillus acidophilus bacterium mud, the mass ratio of mixed lactobacillus acidophilus freeze-drying protective agent each component Are as follows: lactose 5%, oligofructose 5%, proline 2%, galactolipin 5%, trehalose 10%, skimmed milk 20%, surplus are sterile Water；Probiotics bacterium mud is lactobacillus plantarum bacterium mud, the mass ratio of mixed lactobacillus plantarum freeze drying protectant each component Are as follows: trehalose 10%, disodium hydrogen phosphate 0.2%, lactose 1%, skimmed milk 20%, surplus are sterile water；Probiotics bacterium mud is two Qi Bifidobacterium bacterium mud, two mixed qi Bifidobacterium freeze drying protectant each component mass ratios are as follows: glycine 2%, phosphorus Sour hydrogen sodium 0.5%, xylo-oligosaccharide 5%, arginase 12 %, skimmed milk 20%, surplus are sterile water.
Step 2, the preparation of goat milk powder: solid content will be concentrated under reduced pressure into after the purified processing of sheep cream and standardization For 42% concentrated milk, concentrated milk crosses -80 mesh of 40 mesh by being cooled to room temperature after spray drying and obtains goat milk powder；It is spray-dried item Part are as follows: inlet air temperature is 165 DEG C, and temperature of outgoing air is 65 DEG C.
Step 3, prepared by fructus lycii probiotics goat milk piece: it is by mass percentage xylitol 8.58%, antierythrite 2.68%, Mannitol 3.1%, microcrystalline cellulose 0.15%, wolfberry fruit powder 10%, goat milk powder 75.39% and probiotics bacterial powder 0.10% lead to 10 revs/min of revolving speed are crossed, power is that the three-dimensional mixer (SYH-15) of 0.5KW is mixed, and obtains fructus lycii probiotics goat milk powder； Unit viable count is made in fructus lycii probiotics goat milk powder by single-punching tablet press and reaches (1-4) × 107CFU/g~(1-4) × 109CFU/g probiotics goat milk piece；Wherein, the maximum tableting pressure of tablet press machine is 50KN, and pellet diameter isSlice weight is 1g, hardness is in 5kgf.
Embodiment 2
A kind of fructus lycii compound probiotic goat milk piece includes: xylitol 9%, antierythrite 2.67%, sweet dew by its mass ratio Sugar alcohol 3%, microcrystalline cellulose 0.18%, wolfberry fruit powder 15%, goat milk powder 69.15% and probiotics bacterial powder 1%.
A kind of preparation method of fructus lycii compound probiotic goat milk piece, preparation step are as follows:
Step 1, probiotics bacterial powder is prepared: the probiotics after access 2% has activated into culture medium by volume, 37 DEG C of perseverances Temperature culture 21h, and it is primary every 3h stirring；It is then centrifuged for machine and probiotics bacterium mud is made in 8000 turns/min centrifugation 15min centrifugation； Probiotics bacterium mud and freeze drying protectant are uniformly mixed, and are placed in -20 DEG C of cold treatment 15h, obtain freeze-drying probiotics by freeze-drying Bacterium powder；Viable count in the bacterium powder of acquisition are as follows: lactobacillus acidophilus 1.6 × 1011CFU/g, bifidobacterium bifidum 1.4 × 1011CFU/ G and lactobacillus plantarum 2.3 × 1011The bacterium powder of CFU/g, and the ratio mixing of three 1:1:1 in mass ratio；Wherein freeze drying protectant It is lactobacillus acidophilus freeze-drying protective agent, lactobacillus plantarum freeze drying protectant and two qi Bifidobacterium freeze drying protectants by 1:1:1's Volume ratio mixes；
Wherein, the activation process of probiotics specifically: by laboratory save lactobacillus acidophilus, bifidobacterium bifidum and Lactobacillus plantarum freeze-dried vaccine powder accesses in culture medium MRS meat soup, since bifidobacterium bifidum is strictly anaerobic, is cultivating When need to be added 0.05% cysteine hydrochloric acid in MRS meat soup and consume the oxygen in culture medium, be subsequently placed in constant temperature In 37 DEG C of culture 21h in incubator, a small amount of bacteria suspension is taken to carry out microscopy, after determining without living contaminants, presses 5% with asepsis injector Inoculum concentration access MRS meat soup in activation to 3 generations, be put into stored refrigerated spare in refrigerator.
In addition, the mixed proportion of probiotics bacterium mud and freeze drying protectant: 1 gram of probiotics bacterium mud adds 1 milliliter of frozen-dried protective Agent；Probiotics bacterium mud is lactobacillus acidophilus bacterium mud, the mass ratio of mixed lactobacillus acidophilus freeze-drying protective agent each component Are as follows: lactose 10%, oligofructose 10%, proline 5%, galactolipin 10%, trehalose 15%, skimmed milk 40%, surplus are nothing Bacterium water；Probiotics bacterium mud is lactobacillus plantarum bacterium mud, the mass ratio of mixed lactobacillus plantarum freeze drying protectant each component Example are as follows: trehalose 15%, disodium hydrogen phosphate 1%, lactose 5%, skimmed milk 40%, surplus are sterile water；Probiotics bacterium mud is two Qi Bifidobacterium bacterium mud, two mixed qi Bifidobacterium freeze drying protectant each component mass ratios are as follows: glycine 5%, phosphorus Sour hydrogen sodium 1%, xylo-oligosaccharide 10%, arginine 5%, skimmed milk 40%, surplus are sterile water.
Step 2, the preparation of goat milk powder: solid content will be concentrated under reduced pressure into after the purified processing of sheep cream and standardization For 44% concentrated milk, concentrated milk crosses -80 mesh of 40 mesh by being cooled to room temperature after spray drying and obtains goat milk powder；It is spray-dried item Part are as follows: inlet air temperature is 167 DEG C, and temperature of outgoing air is 66 DEG C.
Step 3, prepared by fructus lycii probiotics goat milk piece: being by mass percentage xylitol 9%, antierythrite 2.67% is sweet Reveal sugar alcohol 3%, microcrystalline cellulose 0.18%, wolfberry fruit powder 15%, goat milk powder 69.15% and probiotics bacterial powder 1%.Pass through revolving speed 10 Rev/min, power is that the three-dimensional mixer (SYH-15) of 0.5KW is mixed, and obtains fructus lycii probiotics goat milk powder；Pass through one-shot Fructus lycii probiotics goat milk powder is made unit viable count and reaches (1-4) × 10 by formula tablet press machine7CFU/g~(1-4) × 109CFU/g benefit Raw bacterium goat milk piece；Wherein, the maximum tableting pressure of tablet press machine is 50KN, and pellet diameter isSlice weight is 1.2g, hardness In 6kgf.
Embodiment 3
A kind of fructus lycii compound probiotic goat milk piece includes: xylitol 8%, antierythrite 2%, mannose by its mass ratio Alcohol 3%, microcrystalline cellulose 0.2%, wolfberry fruit powder 13%, goat milk powder 73.3% and probiotics bacterial powder 0.5%.
A kind of preparation method of fructus lycii compound probiotic goat milk piece, preparation step are as follows:
Step 1, probiotics bacterial powder is prepared: the probiotics after access 3% has activated into culture medium by volume, 37 DEG C of perseverances Temperature culture 20h, and it is primary every 3h stirring；It is then centrifuged for machine and probiotics bacterium mud is made in 8000 turns/min centrifugation 15min centrifugation； Probiotics bacterium mud and freeze drying protectant are uniformly mixed, and are placed in -20 DEG C of cold treatment 16h, obtain freeze-drying probiotics by freeze-drying Bacterium powder；Viable count in the bacterium powder of acquisition are as follows: lactobacillus acidophilus 2.3 × 1011CFU/g, bifidobacterium bifidum 1.9 × 1011CFU/ G and lactobacillus plantarum 2.8 × 1011The bacterium powder of CFU/g, and the ratio mixing of three 1:1:1 in mass ratio；Wherein freeze drying protectant It is lactobacillus acidophilus freeze-drying protective agent, lactobacillus plantarum freeze drying protectant and two qi Bifidobacterium freeze drying protectants by 1:1:1's Volume ratio mixes；
Wherein, the activation process of probiotics specifically: by laboratory save lactobacillus acidophilus, bifidobacterium bifidum and Lactobacillus plantarum freeze-dried vaccine powder accesses in culture medium MRS meat soup, since bifidobacterium bifidum is strictly anaerobic, is cultivating When need to be added 0.05% cysteine hydrochloric acid in MRS meat soup and consume the oxygen in culture medium, be subsequently placed in constant temperature In 37 DEG C of culture 20h in incubator, a small amount of bacteria suspension is taken to carry out microscopy, after determining without living contaminants, presses 5% with asepsis injector Inoculum concentration access MRS meat soup in activation to 3 generations, be put into stored refrigerated spare in refrigerator.
In addition, the mixed proportion of probiotics bacterium mud and freeze drying protectant: 1 gram of probiotics bacterium mud adds 1 milliliter of frozen-dried protective Agent；Probiotics bacterium mud is lactobacillus acidophilus bacterium mud, the mass ratio of mixed lactobacillus acidophilus freeze-drying protective agent each component Are as follows: lactose 6%, oligofructose 8%, proline-4 %, galactolipin 6%, trehalose 12%, skimmed milk 24%, surplus are sterile Water；Probiotics bacterium mud is lactobacillus plantarum bacterium mud, the mass ratio of mixed lactobacillus plantarum freeze drying protectant each component Are as follows: trehalose 12%, disodium hydrogen phosphate 0.5%, lactose 3%, skimmed milk 30%, surplus are sterile water；Probiotics bacterium mud is two Qi Bifidobacterium bacterium mud, two mixed qi Bifidobacterium freeze drying protectant each component mass ratios are as follows: glycine 4%, phosphorus Sour hydrogen sodium 0.8%, xylo-oligosaccharide 7%, arginine 4%, skimmed milk 30%, surplus are sterile water.
Step 2, the preparation of goat milk powder: solid content will be concentrated under reduced pressure into after the purified processing of sheep cream and standardization For the concentrated milk of 42-50%, concentrated milk crosses -80 mesh of 40 mesh by being cooled to room temperature after spray drying and obtains goat milk powder；It is spraying dry Dry condition are as follows: inlet air temperature is 169 DEG C, and temperature of outgoing air is 70 DEG C -70 DEG C.
Step 3, prepared by fructus lycii probiotics goat milk piece: it is by mass percentage xylitol 8%, antierythrite 2%, and mannose Alcohol 3%, microcrystalline cellulose 0.2%, wolfberry fruit powder 13%, goat milk powder 73.3% and probiotics bacterial powder 0.5%, by 10 turns of revolving speed/ Minute, power is that the three-dimensional mixer (SYH-15) of 0.5KW is mixed, and obtains fructus lycii probiotics goat milk powder；Pass through one-shot formula Fructus lycii probiotics goat milk powder is made unit viable count and reaches (1-4) × 10 by tablet press machine7CFU/g~(1-4) × 109CFU/g is prebiotic Bacterium goat milk piece；Wherein, the maximum tableting pressure of tablet press machine is 50KN, and pellet diameter isSlice weight is 1.5g, and hardness exists 7kgf。
Embodiment 4
A kind of fructus lycii compound probiotic goat milk piece includes: xylitol 8%, antierythrite 2%, mannose by its mass ratio Alcohol 3%, microcrystalline cellulose 0.15%, wolfberry fruit powder 10%, goat milk powder 76.75% and probiotics bacterial powder 0.1%.
A kind of preparation method of fructus lycii compound probiotic goat milk piece, preparation step are as follows:
Step 1, probiotics bacterial powder is prepared: the probiotics after access 4% has activated into culture medium by volume, 37 DEG C of perseverances Temperature culture 21h, and it is primary every 3h stirring；It is then centrifuged for machine and probiotics bacterium mud is made in 8000 turns/min centrifugation 15min centrifugation； Probiotics bacterium mud and freeze drying protectant are uniformly mixed, and are placed in -20 DEG C of cold treatment 14h, obtain freeze-drying probiotics by freeze-drying Bacterium powder；Viable count in the bacterium powder of acquisition are as follows: lactobacillus acidophilus 3.2 × 1011CFU/g, bifidobacterium bifidum 2.5 × 1011CFU/ G and lactobacillus plantarum 3.4 × 1011The bacterium powder of CFU/g, and the ratio mixing of three 1:1:1 in mass ratio；Wherein freeze drying protectant It is lactobacillus acidophilus freeze-drying protective agent, lactobacillus plantarum freeze drying protectant and two qi Bifidobacterium freeze drying protectants by 1:1:1's Volume ratio mixes；
Wherein, the activation process of probiotics specifically: by laboratory save lactobacillus acidophilus, bifidobacterium bifidum and Lactobacillus plantarum freeze-dried vaccine powder accesses in culture medium MRS meat soup, since bifidobacterium bifidum is strictly anaerobic, is cultivating When need to be added 0.05% cysteine hydrochloric acid in MRS meat soup and consume the oxygen in culture medium, be subsequently placed in constant temperature In 37 DEG C of culture 21h in incubator, a small amount of bacteria suspension is taken to carry out microscopy, after determining without living contaminants, presses 5% with asepsis injector Inoculum concentration access MRS meat soup in activation to 3 generations, be put into stored refrigerated spare in refrigerator.
In addition, the mixed proportion of probiotics bacterium mud and freeze drying protectant: 1 gram of probiotics bacterium mud adds 1 milliliter of frozen-dried protective Agent；Probiotics bacterium mud is lactobacillus acidophilus bacterium mud, the mass ratio of mixed lactobacillus acidophilus freeze-drying protective agent each component Are as follows: lactose 8%, oligofructose 9%, proline 3 %, galactolipin 8%, trehalose 14%, skimmed milk 37%, surplus are sterile Water；Probiotics bacterium mud is lactobacillus plantarum bacterium mud, the mass ratio of mixed lactobacillus plantarum freeze drying protectant each component Are as follows: trehalose 14%, disodium hydrogen phosphate 0.7%, lactose 4%, skimmed milk 35%, surplus are sterile water；Probiotics bacterium mud is two Qi Bifidobacterium bacterium mud, two mixed qi Bifidobacterium freeze drying protectant each component mass ratios are as follows: glycine 3%, phosphorus Sour hydrogen sodium 0.9%, xylo-oligosaccharide 9%, arginine 4.5%, skimmed milk 38%, surplus are sterile water.
Step 2, the preparation of goat milk powder: solid content will be concentrated under reduced pressure into after the purified processing of sheep cream and standardization For 48% concentrated milk, concentrated milk crosses -80 mesh of 40 mesh by being cooled to room temperature after spray drying and obtains goat milk powder；It is spray-dried item Part are as follows: inlet air temperature is 170 DEG C, and temperature of outgoing air is 69 DEG C.
Step 3, prepared by fructus lycii probiotics goat milk piece: it is by mass percentage xylitol 8%, antierythrite 2%, and mannose Alcohol 3%, microcrystalline cellulose 0.2%, wolfberry fruit powder 13%, goat milk powder 73.3% and probiotics bacterial powder 0.5%, by 10 turns of revolving speed/ Minute, power is that the three-dimensional mixer (SYH-15) of 0.5KW is mixed, and obtains fructus lycii probiotics goat milk powder；Pass through one-shot formula Fructus lycii probiotics goat milk powder is made unit viable count and reaches (1-4) × 10 by tablet press machine7CFU/g~(1-4) × 109CFU/g is prebiotic Bacterium goat milk piece；Wherein, the maximum tableting pressure of tablet press machine is 50KN, and pellet diameter isSlice weight is 0.7g, and hardness exists 4kgf。
Embodiment 5
A kind of fructus lycii compound probiotic goat milk piece includes: xylitol 10%, antierythrite 4%, mannose by its mass ratio Alcohol 4%, microcrystalline cellulose 0.2%, wolfberry fruit powder 15%, goat milk powder 65.8% and probiotics bacterial powder 1%.
A kind of preparation method of fructus lycii compound probiotic goat milk piece, preparation step are as follows:
Step 1, probiotics bacterial powder is prepared: the probiotics after access 5% has activated into culture medium by volume, 37 DEG C of perseverances Temperature culture 22h, and it is primary every 3h stirring；It is then centrifuged for machine and probiotics bacterium mud is made in 8000 turns/min centrifugation 15min centrifugation； Probiotics bacterium mud and freeze drying protectant are uniformly mixed, and are placed in -20 DEG C of cold treatment 16h, obtain freeze-drying probiotics by freeze-drying Bacterium powder；Viable count in the bacterium powder of acquisition are as follows: lactobacillus acidophilus 4 × 1011CFU/g, bifidobacterium bifidum 3 × 1011CFU/g and Lactobacillus plantarum 4 × 1011The bacterium powder of CFU/g, and the ratio mixing of three 1:1:1 in mass ratio；Wherein freeze drying protectant is thermophilic Lactobacillus lactis freeze drying protectant, lactobacillus plantarum freeze drying protectant and two qi Bifidobacterium freeze drying protectants press the volume of 1:1:1 Than mixing；
Wherein, the activation process of probiotics specifically: by laboratory save lactobacillus acidophilus, bifidobacterium bifidum and Lactobacillus plantarum freeze-dried vaccine powder accesses in culture medium MRS meat soup, since bifidobacterium bifidum is strictly anaerobic, is cultivating When need to be added 0.05% cysteine hydrochloric acid in MRS meat soup and consume the oxygen in culture medium, be subsequently placed in constant temperature In 37 DEG C of culture 22h in incubator, a small amount of bacteria suspension is taken to carry out microscopy, after determining without living contaminants, presses 5% with asepsis injector Inoculum concentration access MRS meat soup in activation to 3 generations, be put into stored refrigerated spare in refrigerator.
In addition, the mixed proportion of probiotics bacterium mud and freeze drying protectant: 1 gram of probiotics bacterium mud adds 1 milliliter of frozen-dried protective Agent；Probiotics bacterium mud is lactobacillus acidophilus bacterium mud, the mass ratio of mixed lactobacillus acidophilus freeze-drying protective agent each component Are as follows: lactose 6%, oligofructose 7%, proline-4 .5%, galactolipin 9%, trehalose 12%, skimmed milk 33%, surplus are sterile Water；Probiotics bacterium mud is lactobacillus plantarum bacterium mud, the mass ratio of mixed lactobacillus plantarum freeze drying protectant each component Are as follows: trehalose 11%, disodium hydrogen phosphate 0.9%, lactose 3.5%, skimmed milk 25%, surplus are sterile water；Probiotics bacterium mud is Two qi Bifidobacterium bacterium muds, two mixed qi Bifidobacterium freeze drying protectant each component mass ratios are as follows: glycine 4%, Dibastic sodium phosphate 0.7%, xylo-oligosaccharide 7%, arginase 12 .5%, skimmed milk 27%, surplus are sterile water.
Step 2, the preparation of goat milk powder: solid content will be concentrated under reduced pressure into after the purified processing of sheep cream and standardization For 50% concentrated milk, concentrated milk crosses -80 mesh of 40 mesh by being cooled to room temperature after spray drying and obtains goat milk powder；It is spray-dried item Part are as follows: inlet air temperature is 170 DEG C, and temperature of outgoing air is 70 DEG C.
Step 3, prepared by fructus lycii probiotics goat milk piece: it is by mass percentage xylitol 8%, antierythrite 2%, and mannose Alcohol 3%, microcrystalline cellulose 0.2%, wolfberry fruit powder 13%, goat milk powder 73.3% and probiotics bacterial powder 0.5%, by 10 turns of revolving speed/ Minute, power is that the three-dimensional mixer (SYH-15) of 0.5KW is mixed, and obtains fructus lycii probiotics goat milk powder；Pass through one-shot formula Fructus lycii probiotics goat milk powder is made unit viable count and reaches (1-4) × 10 by tablet press machine7CFU/g~(1-4) × 109CFU/g is prebiotic Bacterium goat milk piece；Wherein, the maximum tableting pressure of tablet press machine is 50KN, and pellet diameter isSlice weight is 1.5g, and hardness exists 7kgf。

Claims (9)

1. a kind of fructus lycii compound probiotic goat milk piece, which is characterized in that by its mass ratio include: xylitol 8-10%, erythrose Alcohol 2-4%, mannitol 3-4%, microcrystalline cellulose 0.15-0.2%, wolfberry fruit powder 10-15%, goat milk powder 65.8-76.75% and Probiotics bacterial powder 0.10-1%.
2. a kind of preparation method of fructus lycii compound probiotic goat milk piece, which is characterized in that preparation step is as follows:
Step 1, it prepares probiotics bacterial powder: accessing the probiotics after 1-5% has been activated, 37 DEG C of constant temperature into culture medium by volume 20-22h is cultivated, probiotics bacterium mud is made by centrifuge centrifugation；Freeze drying protectant is added into probiotics bacterium mud to be uniformly mixed, - 20 DEG C of cold treatment 14h-16h are placed in, probiotics bacterial powder is obtained by freeze-drying；
Step 2, the preparation of goat milk powder: sheep cream is concentrated into the concentrated milk that solid content is 42-50%, concentrated milk passes through spraying It is dry to obtain goat milk powder；
Step 3, prepared by fructus lycii probiotics goat milk piece: taking xylitol 8-10%, antierythrite 2-4%, sweet dew by mass percentage Sugar alcohol 3-4%, microcrystalline cellulose 0.15-0.2%, wolfberry fruit powder 10-15%, goat milk powder 65.8-76.75% and probiotics bacterial powder 0.1-1% mixing, obtains fructus lycii probiotics goat milk powder；Probiotics goat milk piece is made by tablet press machine.
3. a kind of preparation method of fructus lycii compound probiotic goat milk piece according to claim 2, which is characterized in that described It includes lactobacillus acidophilus, lactobacillus plantarum and two qi Bifidobacteriums that the probiotics of culture medium is accessed in step 1.
4. a kind of preparation method of fructus lycii compound probiotic goat milk piece according to claim 2, which is characterized in that described Probiotics bacterial powder in step 1 includes lactobacillus acidophilus bacterium powder, lactobacillus plantarum bacterium powder and two qi Bifidobacteria powders, and presses matter Amount with the ratio of 1:1:1 than being mixed.
5. a kind of preparation method of fructus lycii compound probiotic goat milk piece according to claim 2, which is characterized in that described Culture medium in step 1 is MRS meat soup.
6. a kind of preparation method of fructus lycii compound probiotic goat milk piece according to claim 2, which is characterized in that described When cultivating two qi Bifidobacteriums, the half of culture medium quality 0.05% is added into culture medium for the preparation of probiotics bacterial powder in step 1 Cystine hydrochloride.
7. a kind of preparation method of fructus lycii compound probiotic goat milk piece according to claim 2, which is characterized in that described Probiotics bacterium mud is lactobacillus acidophilus bacterium mud, the quality of mixed lactobacillus acidophilus freeze-drying protective agent each component in step 1 Ratio is lactose 5-10%, oligofructose 5-10%, proline 2-5%, galactolipin 5-10%, trehalose 10-15%, skimmed milk 20-40%, surplus are sterile water；Probiotics bacterium mud is lactobacillus plantarum bacterium mud, mixed lactobacillus plantarum frozen-dried protective The mass ratio of agent each component be trehalose 10-15%, disodium hydrogen phosphate 0.2-1%, lactose 1-5%, skimmed milk 20-40%, Surplus is sterile water；Probiotics bacterium mud is two qi Bifidobacterium bacterium muds, and two mixed qi Bifidobacterium freeze drying protectants are each Constituent mass ratio is glycine 2-5%, dibastic sodium phosphate 0.5-1%, xylo-oligosaccharide 5-10%, arginase 12-5%, skimmed milk 20-40%, surplus are sterile water.
8. a kind of preparation method of fructus lycii compound probiotic goat milk piece according to claim 7, which is characterized in that described The mixed proportion of probiotics bacterium mud and freeze drying protectant in step 1 are as follows: 1 gram of probiotics bacterium mud adds 1 milliliter of freeze drying protectant；It is thermophilic Lactobacillus lactis freeze drying protectant, lactobacillus plantarum freeze drying protectant and two qi Bifidobacterium freeze drying protectants press the volume of 1:1:1 Than mixing.
9. a kind of preparation method of fructus lycii compound probiotic goat milk piece according to claim 2, which is characterized in that described The spray drying condition of the step 2 are as follows: inlet air temperature is 165 DEG C -170 DEG C, and temperature of outgoing air is 65 DEG C -70 DEG C.
CN201811621622.0A 2018-12-28 2018-12-28 A kind of fructus lycii compound probiotic goat milk piece and preparation method thereof Pending CN109527099A (en)

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CN112535214A (en) * 2020-12-03 2021-03-23 石家庄君乐宝乳业有限公司 Probiotic milk tablet with intestine regulating effect and preparation method thereof
CN112998081A (en) * 2021-03-17 2021-06-22 星探桔(杭州)网络科技有限公司 Ginseng milk powder and preparation method thereof
CN114958633A (en) * 2022-07-14 2022-08-30 陕西科技大学 Preparation of nutrient-rich probiotic bacteria powder and application of nutrient-rich probiotic bacteria powder in goat milk powder

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CN103918792A (en) * 2014-04-10 2014-07-16 陕西科技大学 Xylitol probiotics goat milk tablets and preparation method thereof
CN105053189A (en) * 2015-07-08 2015-11-18 南阳师范学院 Barbury wolfberry fruit coffee composite fermented goat's milk and preparation method thereof

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CN102626139A (en) * 2012-04-18 2012-08-08 潍坊紫鸢乳业发展有限公司 Method for producing nutrient mushroom, medlar and red date goat milk yogurt stored at normal temperature
CN103918792A (en) * 2014-04-10 2014-07-16 陕西科技大学 Xylitol probiotics goat milk tablets and preparation method thereof
CN105053189A (en) * 2015-07-08 2015-11-18 南阳师范学院 Barbury wolfberry fruit coffee composite fermented goat's milk and preparation method thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112535214A (en) * 2020-12-03 2021-03-23 石家庄君乐宝乳业有限公司 Probiotic milk tablet with intestine regulating effect and preparation method thereof
CN112998081A (en) * 2021-03-17 2021-06-22 星探桔(杭州)网络科技有限公司 Ginseng milk powder and preparation method thereof
CN114958633A (en) * 2022-07-14 2022-08-30 陕西科技大学 Preparation of nutrient-rich probiotic bacteria powder and application of nutrient-rich probiotic bacteria powder in goat milk powder
CN114958633B (en) * 2022-07-14 2023-10-13 浙江忠梦昌健康科技有限公司 Preparation of nutrient-rich probiotic bacteria powder and application of nutrient-rich probiotic bacteria powder in goat milk powder

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Application publication date: 20190329