CN104489646B - A kind of fruit and vegerable probiotic tablet and preparation method thereof - Google Patents

A kind of fruit and vegerable probiotic tablet and preparation method thereof Download PDF

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CN104489646B
CN104489646B CN201410658354.5A CN201410658354A CN104489646B CN 104489646 B CN104489646 B CN 104489646B CN 201410658354 A CN201410658354 A CN 201410658354A CN 104489646 B CN104489646 B CN 104489646B
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CN104489646A (en
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李雅君
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Nanjing Xuyou Food Technology Co.,Ltd.
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/123—Bulgaricus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/125—Casei
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/21—Streptococcus, lactococcus
    • A23V2400/249—Thermophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/513—Adolescentes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/517—Bifidum
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/519—Breve
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/529—Infantis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/533—Longum

Abstract

The invention discloses a kind of fruit and vegerable probiotic tablet and preparation method thereof, with probiotic bacteria powder such as Lactobacillus plantarum as primary raw material, the composite modified dietary fiber of science, fruit vegetable powder, oligosaccharide, plant extract, egg albumen powder, Folium Camelliae sinensis extract, Chinese herbal medicine extract etc., improve the content of the soluble cellulose that beneficial flora is had real meaning, enhance the physiologically active of cellulose, and then while increasing beneficial bacteria of intestinal tract flora kind, significantly enhance endogenous and that external source probiotic bacteria is in human body intestinal canal colonization ability and resident time, effectively inhibit growth and the breeding of harmful intestinal tract bacteria especially gram negative bacteria, fully have adjusted the composition of beneficial bacteria of intestinal tract flora, the fruit and vegerable probiotic tablet biological activity of preparation is high, long at human body intestinal canal resident time, fat-reducing effect is notable and suitable population is extensive.

Description

A kind of fruit and vegerable probiotic tablet and preparation method thereof
Technical field
The present invention relates to probiotic tablet, be specifically related to a kind of fruit and vegerable probiotic tablet with antiobesity action and preparation method thereof.
Background technology
Probiotic bacteria (Probiotics), also known as probiotics, regulator, raw rhzomorph, viable bacteria element etc., i.e. refer to certain microorganism formulation or fermented product, by regulating host's mucosa and systemic immune function and improving enteral nutrition and colony balance, host is produced useful physiological action, changed the composition of host a certain position flora by field planting effect, thus produce and be conducive to the single of host health effect or form clear and definite mixed microorganism.Up to now, the probiotic bacteria that scientist has been found that generally is divided into three major types, including: 1. lactobacillus class (such as bacillus acidophilus, lactobacillus casei, Lactobacillus bulgaricus, lactobacillus plantarum etc.)；2. bacillus bifidus class (such as bifidobacterium longum, bifidobacterium adolescentis, bifidobacterium breve, bifidobacteria infantis etc.)；3. gram-positive cocci (such as streptococcus faecalis, Lactococcus, intermediary streptococcus etc.).As a frontier, in recent years, the special physiological activity of probiotic bacteria is widely used in food, in addition to being applied to fermented dairy product, is the most progressively applied to developing various functional food.At present, probiotic bacteria is mainly used in the fields such as food, functional food, dietary supplement, medicinal organism goods and feedstuff.Food containing profitable probliotics is referred to as probiotic food, the food that mainly includes directly adding probiotic bacteria and the food through probiotics fermention.Probiotic bacteria most common application in field of food is still milk product field, including fermentation milk, active lactic acid bacteria drink, cheese, sour cream, kefyr, fruit juice, cereals, cookies etc..Probiotic bacteria strain have bacillus bifidus (Bifidobateria), lactobacillus and some streptococcus, wherein, what lactobacillus was conventional has bacillus acidophilus (Lactobicullus acidophilus), lactobacillus casei (L.casei), Lactobacillus bulgaricus (L.bulgaricus) etc..
At present, research about probiotic tablet is more: Chinese patent CN 101700253B discloses a kind of probiotic tablet and preparation method thereof, with glucose, lactose, microcrystalline Cellulose, the adjuvants such as defatted milk powder are that granule prepared by raw material, remix active ingredient probiotic bacteria powder and magnesium stearate carries out tabletting, finally give the production method of probiotic tablet and the high probiotic tablet of a kind of Viable detection of applying this kind of method to produce, advantage is: the probiotic tablet of preparation is high because of its Viable detection, taking convenience, it is easy to absorb, therapeutic effect is obvious, and the non-slurry pelletizing technology that the present invention provides has low cost, discharging speed is fast, stable process conditions, favorable reproducibility, probiotic bacteria survival rate advantages of higher, it is suitable for large-scale production.Chinese patent CN 103876145A discloses a kind of probiotics micro-ecological tablet and preparation method thereof, belongs to the technical field of health food and processing.Probiotics micro-ecological tablet is probiotics bacterial 90%～98% by mass, oligofructose 1%～9%, magnesium stearate 0.1%～1%.Preparation is to be activated by bacillus bifidus, and prepares Kefir fermenting agent；Be dried by the two blended fermentation cryoconcentration, batch mixing tabletting prepares again.Mixed fermentation is to be inoculated by probiotic bacteria mixed bacteria, ferments 16～24 hours under the conditions of 28～36 DEG C, Kefir fermenting agent and the ratio 1～3:1 of bacillus bifidus in mixed bacteria.Kefir fermenting agent and bacillus bifidus are carried out high density mixed fermentation by the present invention, and strain is various, stable storing, and the viable count in tablet respectively reaches 2,000,000,000 more than cfu/g；It is possible not only to improve digestion power, also enhances probiotic bacteria colonization ability in intestinal simultaneously；Have and be of high nutritive value, the ability such as unique flavor.Application publication number is that the Chinese patent of CN 102348388A discloses a kind of stable probiotic granulate and preparation method thereof, relates to the probiotic composition field of people and/or animal foodstuff and health product.The method of granulating that the compositions that the invention particularly relates to and comprise probiotic micro-organisms is relevant, for producing the compositions containing stable probiotic granulate, described probiotic granulate keeps stable producing during the tablet or granule of final use.The working result of present invention applicant shows, during granulation, the mortality rate of probiotic micro-organisms is closely related with the characteristic of granulation procedure condition and selected microorganism.Described work establishes the mathematic(al) representation for limiting yeast character and method parameter so that after granulation, microorganism loss is less than the 1log CFU/g of granular food.Chinese patent CN 10155908A discloses the preparation method of the probiotics preparation of a kind of blood fat reducing and regulating intestinal canal flora, and Kefir lactobacillu plantarurn MA2 is carried out High Density Cultivation for (1) by its step, obtains fermentation liquid, and its cell density is not less than 108cfu/mL；(2) the centrifugal collection of above-mentioned fermentation liquor obtains thalline, adds protective agent, is lyophilized into mycopowder；(3) adding oligosaccharide mixture in the mycopowder of lyophilizing, mix homogeneously i.e. obtains probiotics preparation finished product.These product also have the physiological function of regulating intestinal canal flora while having hypolipemic function, can treat and prevent hyperlipidemic conditions and regulating intestinal canal flora, and nontoxic, safety, long shelf-life, can be made into capsule, microcapsule, electuary, tablet etc..
Patent disclosed above is concentrated mainly on to increase probiotic bacteria kind, improve tablet viable bacteria content, treatment cardiovascular disease, looks improving and the skin nourishing, improve in the product for the purpose of stability etc. and preparation method, although having their own characteristics each, but the microcrystalline Cellulose used is mainly with timber, Cotton Gossypii, cotton linter, wheat straw, Caulis et Folium Oryzae, phragmites communis, fiber crops, Cortex Mori, Folium et Cacumen Broussonetiae Kazinoki and bagasse etc. for raw material through slurrying, bleaching, mechanical dispersion, prepared by the Industrialized synthesis methods such as pulverizing, there is bigger food safety hidden danger, the probiotic products research with weight losing function is less, at present, fat more and more significant on the impact of human health, therefore genesis mechanism and the therapeutic strategy of studying obesity are the most necessary.Research shows, intestinal microbial population can help to obtain in host's polysaccharide component from diet more calorie, and is stored in adipose cell standby.Intestinal microbial population can also increase fat storage in fatty tissue and liver by closing the expression of Fiaf gene and increase ChREBP/SREBP, Acc1 and Fas.Further, the lipopolysaccharide LPS that in intestinal, gram negative bacteria produces, has caused whole body chronic low-level inflammation to react, has then led to obesity.Therefore, it is expected to become the effective ways that future therapeutic is fat by probiotic bacteria, prebiotics or antibiotics modification intestinal microbial population.
Summary of the invention
nullSolved by the invention technical problem is that the deficiency overcoming existing probiotic tablet，With probiotic bacteria powder such as Lactobacillus plantarum as primary raw material，The composite modified dietary fiber of science、Fruit vegetable powder、Oligosaccharide、Plant extract、Egg albumen powder、Folium Camelliae sinensis extract、Chinese herbal medicine extract etc.，Improve the content of the soluble cellulose that beneficial flora is had real meaning，Enhance the physiologically active of cellulose，And then while increasing beneficial bacteria of intestinal tract flora kind，Significantly enhance endogenous and that external source probiotic bacteria is in human body intestinal canal colonization ability and resident time，Effectively inhibit growth and the breeding of harmful intestinal tract bacteria especially gram negative bacteria，Fully have adjusted the composition of beneficial bacteria of intestinal tract flora，Prepare a kind of biological activity high、Long at human body intestinal canal resident time、Fat-reducing effect is notable and suitable population's fruit and vegerable probiotic tablet widely.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of fruit and vegerable probiotic tablet, is prepared by the raw material of following parts by weight:
Probiotic bacteria powder 60-80 part, modified dietary fiber 35-45 part, fruit vegetable powder 25-35 part, oligosaccharide 20-30 part, plant extract 25-30 part, egg albumen powder 18-25 part, Folium Camelliae sinensis extract 15-20 part, Chinese herbal medicine extract 8-10 part, sweeting agent 2-5 part, magnesium stearate 0.2-0.5 part；
Described probiotic bacteria powder probiotics viable bacteria content is: 7x1012-9x1012cfu/g；
nullFurther，Described probiotic bacteria powder includes but not limited to one or more in following probiotic bacteria powder: such as: Lactobacillus plantarum (Lactobacillus plantarum)、Bifidobacterium bifidum (Bifidobacterium bifidum)、Bifidobacterium infantis (B.infantis)、Long bifidus bacillus (B.longum)、Short bifidus bacillus (B.breve)、Bifidobacterium adolescentis (B.adolescentis)、Lactobacillus bulgaricus (Lactobacillus.Bulgaricus)、Bacillus acidophilus (L.acidophilus)、Lactobacillus casei (Lactobacillus casei)、Streptococcus thermophilus (Streptococcus thermophilus)；nullAlso one or more in the fungus powder of health food are included，Such as: saccharomyces cerevisiae (Saccharomyces cerevisiae)、Candida utilis (Cadida atilis)、Kluyveromyces lactis (Kluyveromyces lactis)、Saccharomyces carlsbergensis (Saccharomyces carlsbergensis)、Peacilomyce hepiahi (Paecilomyces hepiali Chen et Dai,sp.nov)、Hirsutella hepiali Chen et Shen (Hirsutella hepiali Chen et Shen)、Ganoderma (Ganoderma lucidum)、Ganoderma (Ganoderma sinensis)、Ganoderma tsugae (Ganoderma tsugae)、Monascus (Monacus anka)、Mauve aspergillar (Monacus purpureus)；
Preferably, described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 45-55 part, bifidobacterium longum 35-45 part, bifidobacterium bifidum 25-35 part, bifidobacterium infantis 25-35 part, bifidobacterium adolescentis 15-20 part, Lactobacillus bulgaricus 15-20 part, bacillus acidophilus's 10-15 part, lactobacillus casei 8-10 part, streptococcus thermophilus 8-10 part, saccharomyces cerevisiae 5-10 part, Candida utilis 4-6 part；
Preferably, described Lactobacillus plantarum powder is to be prepared by Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 2nd, 2014 and (is called for short CGMCC, address is: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3. Institute of Microorganism, Academia Sinica. and postcode: 100101), deposit number is CGMCC NO.9405, and Classification And Nomenclature is: Lactobacillus plantarum Lactobacillus plantarum.
Further, described modified dietary fiber is that dietary fiber is processed through physics, chemical or biological method and obtained, soluble fiber cellulose content is high, biological activity is strong, have important, the cellulose of positive role to beneficial bacteria of intestinal tract group, compared with full diet fiber, its biological action is more powerful: 1) soluble fiber cellulose content is high, it is easier to be utilized by probiotic bacteria, probiotic bacteria can be improved in the growth of intestinal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH value, improve intestine microenvironment；2) powerful absorbability, after modified, the specific surface area of cellulose increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability chelating, adsorbing cholesterol and bile acids is higher, the suppression human body absorption to them；3) ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher；4) retentiveness, dilatancy, thickening property be higher and acid and alkali, alkali, the impact of salt；
Preferably, described modified fibre is to be obtained through enzyme enzymolysis by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber, Testa oryzae, wheat bran；
More preferably, the preparation method of described modified dietary fiber comprises the following steps: uniformly mixed by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber, Testa oryzae, wheat bran 7-12:6-9:3-5:2-4:1-3:1-2 in mass ratio, add the water of its quality 3-7 times, room temperature 200-300W, 35-40KHz condition supersound extraction 10-15min, simultaneously in electric field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under the conditions of pulse frequency 200-300Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution concentrating under reduced pressure, lyophilization, low-temperature grinding to granularity are that 0.1-0.3mm i.e. obtains modified dietary fiber；
Described enzyme is that xylanase, cellulase, laccase, pectase, tannase, amylase and protease 6-8:4-7:3-5:2-4:1-3:1-3:0.3-0.5 in mass ratio uniformly mixes.
Further, one or more during described fruit vegetable powder is Malpighia glabra L. powder, Fructus Citri Limoniae powder, Folium Allii tuberosi powder, cucumber powder, white radish powder, white gourd powder, Semen Phaseoli radiati Germinatus powder, Fructus Fragariae Ananssae powder, Radix Dauci Sativae powder, celery powder, papaya powder, Fructus Ananadis comosi powder, orange powder, Citrus powder, Fructus Citri sinensis powder, coconut slurry powder, cranberry powder, blue berry powder uniformly mix；
Preferably, the mass percent of described fruit vegetable powder consists of: Malpighia glabra L. powder 15%, Fructus Citri Limoniae powder 13%, Folium Allii tuberosi powder 10%, celery powder 7%, white gourd powder 7%, Fructus Fragariae Ananssae powder 5%, Radix Dauci Sativae powder 5%, papaya powder 5%, Fructus Ananadis comosi powder 5%, Semen Phaseoli radiati Germinatus powder 5%, orange powder 3%, Citrus powder 3%, Fructus Citri sinensis powder 3%, coconut slurry powder 4%, cranberry powder 3%, cucumber powder 3%, white radish powder 2%, blue berry powder 2%；
Preferably, the preparation method of each fruit vegetable powder described is: is put into by fruit and vegerable in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and cleans 10-15min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, put and microwave dryer carries out under the conditions of power 3000W, frequency 2000Hz, temperature 50-60 DEG C microwave drying 6-12min, until moisture is down to 5-8%, then in 20-30 DEG C, be crushed to granularity 0.1-0.3mm, pack and i.e. obtain fruit vegetable powder.
Further, during described oligosaccharide is oligofructose, stachyose, Raffinose, oligomeric xylose, oligomeric galactose, soybean oligo saccharide, oligomeric isomaltose, Oligomeric maltose at least one；
Preferably, described oligosaccharide parts by weight consist of oligofructose 40-50 part, stachyose 30-40 part, Raffinose 20-40 part, oligomeric xylose 16-18 part, oligomeric galactose 10-15 part, soybean oligo saccharide 10-15 part, oligomeric isomaltose 10-15 part, Oligomeric maltose 8-12 part.
Further, described plant extract is can remarkably promote lactobacter growth, increases the natural plants of viable lactic acid bacteria content and the higher raw-food material of leptin content extracts through science and prepares；
nullPreferably，The preparation method of described plant extract is: count by weight，Accurately weigh Jerusalem artichoke 30-50 part，Rhizoma Dioscoreae 30-40 part，Fructus Momordicae charantiae 20-40 part，Semen Armeniacae Amarum 20-40 part，Fructus Arctii 20-30 part，Semen Nelumbinis 15-25 part，Semen Cassiae 15-20 part，Bulbus Allii Cepae 10-15 part，Germinatus Phragmitis 5-10 part，Being crushed to particle diameter is less than 2 millimeters，Then in container, uniformly mix and add the water of 5 times of weight of mixture，Control temperature 80 DEG C and keep 3h，Then 50 DEG C it are cooled to，The mixing enzyme preparation adding mixed material gross weight 8% carries out enzymolysis，It is 6.2 with apple vinegar regulation pH value，Enzymolysis 3h，Finally add 1.8 times of w ethanol of mixed material and the mixture of propanol，The mass ratio of ethanol and propanol mixing is 1:2，Control temperature and keep 4h to 70 DEG C，Filter，Filtrate reduced in volume、Lyophilization、Low-temperature grinding is to granularity 0.1-0.3mm,Then the vitamin D of ground product quality 0.01-0.03% is added,Mix homogeneously i.e. obtains plant extract；
The parts by weight of described mixed enzyme consist of: xylanase 18 parts, pentosanase 18 parts, glucanase 15 parts, lipase 15 parts, pullulanase 10 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucoseoxidase 6 parts, acid phosphatase 8 parts；
Described concentration of alcohol is 75%.
Further, one or more during described egg albumen powder is Yoghourt powder, soybean protein, casein, lactalbumin；
Preferably, described egg albumen powder percentage by weight consists of: Yoghourt powder 40%, lactalbumin 35%, soybean protein 15%, casein 10%；
Described Yoghourt powder is commercial commodity, and viable lactic acid bacteria content is 3x1011-5x1011cfu/g。
Further, the preparation method of described Folium Camelliae sinensis extract in turn includes the following steps: is put into by commercially available Folium Camelliae sinensis in the ultrasonic washing unit equipped with 0.8% sodium bicarbonate solution and cleans 10-15min in 200W, 40KHz, then in the sterile malt dextrin in aqueous solution of 0.25-0.35MPa pressure lower surface even application mass percent concentration 0.5-1.5%, Folium Camelliae sinensis with the mass ratio of maltodextrin aqueous solution is: 100:0.5-15, seal, rehydration 2-8h under room temperature；Then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, granularity is 0.1-2mm；Folium Camelliae sinensis after pulverizing is placed in pill tank, adds the sterilized water of tea quality 5-10 times, room temperature 200W, 40KHz condition supersound extraction 10-15min；Simultaneously in electric field intensity 20-40kV/cm, burst length 400-500 μ s, under the conditions of pulse frequency 200-300Hz, carry out high-pressure pulse electric extraction；Finally by said extracted liquid breast acid for adjusting pH value be 4.5-6.5, add mixture quality 0.5-1.5% compound enzyme, in 35-45 DEG C of low temperature enzymolysis 4-12h；Enzymolysis solution filtration, filtrate reduced in volume, lyophilization, low-temperature grinding to granularity are that 0.1-0.3mm i.e. obtains Folium Camelliae sinensis extract；
Described compound enzyme is one or more in pectase, cellulase, tannase, protease；
Preferably, the mass ratio of described pectase, cellulase, tannase and protease mixing is 1-2:2-4:2-4:1-3.
Further, the preparation method of described Chinese herbal medicine extract is: count by weight, accurately weighs Folium Artemisiae Argyi 50-55 part, Bulbus Allii 50-55 part, Herba portulacae 45-55 part, Herba Houttuyniae 45-55 part, Radix Scutellariae 30-45 part, Cortex Phellodendri 30-40 part, Fructus Crataegi 30-40 part, Radix Glycyrrhizae 25-35 part, Radix Sophorae Flavescentis 15-25 part, Rhizoma Alismatis 8-10 part, Rhizoma Polygoni Cuspidati 6-8 part, Radix Et Rhizoma Rhei 2-5 part, Semen Sinapis Albae 2-5 part, Radix Polygoni Multiflori 1-3 part；It is less than 2 millimeters that said herbal medicine is crushed to particle diameter respectively, then in container, uniformly mix and add the water of 5 times of weight of mixture, control temperature 80 DEG C and keep 3h, be then cooled to 50 DEG C, the compound enzyme adding mixed material gross weight 8% carries out enzymolysis, it is 6.2 with breast acid for adjusting pH value, enzymolysis 3h, finally adds 1.8 times of w ethanol of mixed material and the mixture of propanol, the mass ratio of ethanol and propanol mixing is 1:2, control temperature and keep 4h to 70 DEG C, filter, obtain the first filtrate；Add the water of 2 times of weight of filtering residue, control temperature 90 DEG C and keep 2h, be then cooled to 30 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate being merged according to mass ratio 2.5:1.5, concentrating under reduced pressure, lyophilization, low-temperature grinding to granularity are that 0.1-0.3mm i.e. obtains Chinese herbal medicine extract；
The parts by weight of described compound enzyme consist of: endo-beta-glucanase 20 parts, outer 1,4 beta-glucanase 18 parts, beta-glucosidase 12 parts, xylanase 18 parts, pentosanase 18 parts, pullulanase 25 parts, beta amylase 12 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucoseoxidase 6 parts, acid phosphatase 8 parts, lipase 6 parts；
Described concentration of alcohol is 75%.
Further, one or more during described sweeting agent is erythritol, xylitol, maltose alcohol, Sorbitol, stevioside, mogroside, aspartame mix with arbitrary proportion.
The preparation method of described fruit and vegerable probiotic tablet, comprise the steps: accurately to weigh each component raw material by above-mentioned formula, first probiotic bacteria powder and modified dietary fiber are uniformly mixed 20-30min, then Chinese herbal medicine extract it is sequentially added into, Folium Camelliae sinensis extract, egg albumen powder, plant extract, oligosaccharide, fruit vegetable powder uniformly mixes 10-15min, add mixture into dry granulating machine pelletize, collect the granule of 10-100 mesh, it is sequentially added into sweeting agent, the raw material of directly compressible is obtained after magnesium stearate mixing, put into tabletting machine, regulation pressure is 10-40kn, obtain fruit and vegerable probiotic tablet.
In above-mentioned preparation method, fruit and vegerable probiotic bacteria can be pressed into diameter 6～12mm, tablet weight 0.3～0.5g, hardness 8～10kg/cm2Tablet.
Described fruit and vegerable probiotic tablet probiotics viable bacteria content is: 6x1011-8x1011cfu/g。
Fruit and vegerable probiotic tablet of the present invention can be used for daily supplementary human body probiotic bacteria, it is possible to loses weight accesary foods as obesity patient, it is also possible to use as raw-food material.
The present invention another object is that the application in fat-reducing of the described fruit and vegerable probiotic tablet.
Operation instruction:
1. product specification: 32 x 0.4g；
The most daily probiotic bacteria supplement: warm water be administered orally, one time 0.4-0.8 gram, 2 times on the one.
3. obesity patient: warm boiled water, be grown up one time 0.8-2 gram, three times on the one.
4. suitable population: not yet find to be not suitable for crowd.
Lactobacillus plantarum of the present invention (Lactobacillus plantarum) tlj-2014 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 2nd, 2014 and (is called for short CGMCC, address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3. Institute of Microorganism, Academia Sinica. and postcode: 100101), deposit number is CGMCC NO.9405, and Classification And Nomenclature is: Lactobacillus plantarum Lactobacillus plantarum.
Described lactobacillus plantarum strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, atrichia, does not produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.
Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+).
Described Lactobacillus plantarum uses following flow process to carry out selection-breeding:
The original strain that sets out → test tube activation → dithyl sulfate (DES) mutation → nitrosoguanidine (NTG) mutation → plasma mutation → flat board primary dcreening operation → shaking flask sieves → mitotic stability test again.
Described starting strain is in MRS dextrose culture-medium, and the throughput rate of its lactic acid is 1.5g/L/d, almost stops growing when medium pH is 3.5, and the decomposition rate to sodium nitrite is 0.34mg/h/kg Chinese cabbage.Starting strain is the greenfeed that Li Zheng is collected in Fattening Sheep field, Yanchi county Ningxia, acquisition time JIUYUE in 2013 15 days.
In order to improve its production of lactic acid speed, acid-fast ability and the decomposition rate of nitrite, use DES and NTG technology that this strain is carried out mutation successively, after mutation, bacterial strain uses MRS calcium carbonate flat board to carry out primary dcreening operation, then 500mL shake flask fermentation is used, biosensor analysis instrument carries out multiple sieve to Producing Strain, the lactobacillus plantarum strain that selection-breeding is excellent, then does passage assays, evaluates its hereditary stability.
Lactobacillus plantarum tlj-2014 hereditary stability result shows: through continuous passage ten times, property indices is the most more stable, and heritability is preferable, and character is not replied, the purpose bacterial strain therefore Lactobacillus plantarum tlj-2014 obtained as selection-breeding.
Empirical tests finds: the production of lactic acid speed of this mutagenic strain can reach 35g/L/d, and this bacterial strain lactic acid concn after fermentation in 71 hours reaches 95g/L；Can survive under conditions of pH is 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of natural fermentation process nitrite accumulation is about 1.1mg/h/kg), it is possible to resistance to 1% cholate.
Therefore using this strain to produce Pickles, whole sweat nitrite concentration is at below 5mg/kg, far below the content (20mg/kg) of regulation in standard GB/T 2714-2003.
Beneficial effect:
Fruit and vegerable probiotic tablet of the present invention is with probiotic bacteria powder such as Lactobacillus plantarum as primary raw material, the composite modified dietary fiber of science, fruit vegetable powder, oligosaccharide, plant extract, egg albumen powder, Folium Camelliae sinensis extract, Chinese herbal medicine extract etc., improve the content of the soluble cellulose that beneficial flora is had real meaning, enhance the physiologically active of cellulose, and then while increasing beneficial bacteria of intestinal tract flora kind, significantly enhance endogenous and that external source probiotic bacteria is in human body intestinal canal colonization ability and resident time, effectively inhibit growth and the breeding of harmful intestinal tract bacteria especially gram negative bacteria, fully have adjusted the composition of beneficial bacteria of intestinal tract flora, the fruit and vegerable probiotic tablet biological activity of preparation is high, long at human body intestinal canal resident time, fat-reducing effect is notable and suitable population is extensive.The powerful network of modified dietary fiber the most of the present invention and absorption affinity, probiotic powder is wrapped up wherein, add the bacteriostasis of Chinese herbal medicine extract, the antioxidation etc. of Folium Camelliae sinensis extract enhances the stability of fruit and vegerable probiotic tablet, improve the probiotics viable bacteria survival rate of tablet, airtight preservation at a temperature of 25 DEG C, conventionally detect in tablet viable count after placing 12 months and to calculate survival rate be 83-89%, improve 24-30% than existing probiotic tablet Viable detection；Being shown by heat resistant test, simulated gastric fluid and intestinal juice resistance test, simulation cholate resistance test, the probiotic bacteria in tablet can keep higher Viable detection, and its Viable detection can reach 88-99%, probiotic significantly；Show through mouse intestinal performance test: probiotic tablet treatment group Mouse Weight average rate of increase (53.31%) is significantly higher than natural recovering group (32.14%)；Feed sheet agent solution rear intestinal escherichia coli quantity to be remarkably decreased, reduce by 80.89%, it is substantially less than natural recovering group (24.78%), show the rapid field planting in white mice intestinal of the probiotic bacteria in fruit and vegerable probiotic tablet of the present invention, form dominant microflora, and the growth and breeding of effective suppression pathogen such as escherichia coli, and resident time is long, continues, effectively improves intestinal performance；Suitable population is extensive, fat-reducing effect is good, and after taking a cycle (30 days), it is 95 people that body weight reduces the number of more than 1-10kg, fat-reducing effect has reached more than 95%, and wherein body weight reduces 1-5kg, 5-10kg, more than 10kg are respectively 53%, 26% and 16%；The more significant age bracket of fat-reducing effect be 25-50 year more than, wherein body weight reduce the crowd of more than 10kg be mainly 25-30 year, be secondly 30-50 year crowd；The population of weight of more than 50 years old reduces main at below 10kg, it is seen then that fruit and vegerable probiotic tablet of the present invention can be suitable for the overweight people of various all ages and classes and eat, and has significant fat-reducing effect；Meanwhile, the severe obesity person reducing more than 10kg accounts for 37.5%, and central obesity person accounts for 37.5%, and Mild Obesity person accounts for 25%；The severe obesity person reducing 1-5kg accounts for 5.66%, and central obesity person accounts for 26.42%, and Mild Obesity person accounts for 67.92%, the fat-reducing effect of fruit and vegerable probiotic tablet alignment degree of the present invention and severe obesity person is described more preferably.
It is in particular in:
Fruit and vegerable probiotic tablet probiotic bacteria kind the most of the present invention is many, and Lactobacillus plantarum CGMCC NO.9405 production of lactic acid speed therein can reach 35g/L/d, and this bacterial strain lactic acid concn after fermentation in 71 hours reaches 95g/L；Can survive under conditions of pH is 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of natural fermentation process nitrite accumulation is about 1.1mg/h/kg), it is possible to resistance to 1% cholate, its prebiotic performance is more superior.
2. supersound extraction, high-pressure pulse electric are extracted and biological enzymolysis combination of sciences by the modified dietary fiber of the present invention, gained modified dietary fiber soluble fiber cellulose content is high, it is easier to be utilized by probiotic bacteria, improve probiotic bacteria in the growth of intestinal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH value, improve intestine microenvironment；High adsorption capacity, after modified, the specific surface area of cellulose increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability chelating, adsorbing cholesterol and bile acids is higher, the suppression human body absorption to them；Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher, effectively prevent body weight for humans metal poisoning；Retentiveness, dilatancy, thickening property be higher and acid and alkali, alkali, the impact of salt.
Chinese herbal medicine extract the most of the present invention with significantly inhibit gram negative bacteria Chinese herbal medicine as raw material, water extract-alcohol precipitation and biological enzymolysis is used to prepare, active constituent content is high, effectively instead of the utilization of antibiotic, improve body immunity, strengthening the macrophage phagocytosis to harmful bacteria, it is suppressed that the growth of harmful intestinal tract bacteria and breeding, the most also growth and breeding for probiotic bacteria provide guarantee.
Microwave drying and low-temperature grinding are combined by fruit vegetable powder the most of the present invention, the nutrient substance such as the color and luster own and cellulose, vitamin, the fructose that remain high microsteping fruit and vegerable to greatest extent, good, with short production cycle compared with the quality of production of other fruit vegetable powder, efficiency is high, be conducive to probiotic bacteria to utilize and strengthen intestinal peristalsis promoting, be conducive to excreting harmful bacteria and noxious substance, prevent the generation of constipation and symptom of diarrhea.
Plant extract the most of the present invention is can remarkably promote lactobacter growth, increase the natural plants of viable lactic acid bacteria content and the higher raw-food material of leptin content prepares through water extract-alcohol precipitation and biological enzymolysis, use apple vinegar regulation pH value, use most scientific extracting method, remain effective ingredient therein to greatest extent, leptin is replaced with raw-food material, with appetite-suppressing, strengthen capacity of self-regulation, reduce the external absorption of fat and internal synthesis, enhance fat-reducing effect, the most also provide nutrient substance comprehensive, abundant for beneficial bacteria of intestinal tract.
Folium Camelliae sinensis extract the most of the present invention not only can provide the factor of effectively losing weight, and containing abundant Natural Antioxidants, improve the oxidation resistance of human body, eliminate free radical, improve immunity of organisms, delaying body aging, slow down the defying age burden of intestinal microbial population, beneficially intestinal microbial population is grown up healthy and sound.
The probiotic effects of fruit and vegerable probiotic tablet the most of the present invention and the simple combination of the most each notable component function of fat-reducing effect, but science is compounding, synergistic comprehensive embodiment, mainly in terms of increasing probiotic bacteria kind and quantity, increase probiotic bacteria nutrient substance, suppression harmful bacteria growth and increasing body immunity, modify intestinal microbial population, improve the probiotic and fat-reducing effect of product.
The powerful network of modified dietary fiber the most of the present invention and absorption affinity, probiotic powder is wrapped up wherein, add the bacteriostasis of Chinese herbal medicine extract, the antioxidation etc. of Folium Camelliae sinensis extract enhances the stability of fruit and vegerable probiotic tablet, improves the probiotics viable bacteria survival rate of tablet.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, on the premise of without departing substantially from spirit and scope of the present invention, the various changes or the change that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.
Prepared by embodiment 1 raw material
1. the preparation of Lactobacillus plantarum powder:
(1) first order seed is cultivated: accessed by Lactobacillus plantarum CGMCC NO.9405 strain in 500 milliliters of shaking flasks, seed culture medium loading amount 100 milliliters, cultivation temperature 37 DEG C, incubation time 24 hours；
(2) secondary seed is cultivated: being accessed in 500 milliliters of secondary seed shaking flasks according to the inoculum concentration of 10% by first order seed, condition of culture is identical with first order seed；
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, seed culture medium loading amount 1000 milliliters, cultivation temperature 37 DEG C, incubation time 24 hours；
(4) first class seed pot is cultivated: with 5% inoculum concentration, three grades of seeds are accessed the total measurement (volume) first class seed pot as 150L, fermentation medium loading amount 100L, cultivation temperature 37 DEG C, tank pressure 0.05MPa, incubation time 18 hours；
(5) fermentor cultivation: with 5% inoculum concentration, first class seed pot strain is accessed total measurement (volume) is 3 tons of secondary seed tanks, fermentation medium loading amount 2 tons, condition of culture cultivation temperature 37 DEG C, tank pressure 0.05MPa, incubation time 22 hours.The complete fermentation liquid that ferments staticly settles, centrifugal be precipitated thing, then adds the carrier of precipitate quality 0.8 times, and mix homogeneously, 50 DEG C of fluid bed dryings i.e. obtain Lactobacillus plantarum powder, and vehicle weight consists of: Yoghourt powder 25 parts, 12 parts of dextrin.
Described seed culture medium quality group becomes: casein 1%, Carnis Bovis seu Bubali cream 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamidogen 0.2%, Tween 800.1%, K2HPO40.2%, MgSO4.7H2O 0.02%, MnSO4.H2O 0.005%, CaCO32%, surplus is water, pH6.8.
Described fermentation medium quality group becomes: soybean protein 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamidogen 0.2%, K2HPO40.2%, MgSO4.7H2O 0.02%, MnSO4.H2O 0.005%, CaCO32%, surplus is water, pH6.8.
In described Lactobacillus plantarum powder, viable bacteria content is 9x1012cfu/g。
2. the preparation of modified dietary fiber
The preparation method of described modified dietary fiber comprises the following steps: uniformly mixed by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber, Testa oryzae, wheat bran 10:7:4:3:2:1.5 in mass ratio, add the water of its quality 5 times, room temperature 250W, 40KHz condition supersound extraction 12min, simultaneously in electric field intensity 30kV/cm, burst length 500 μ s, carries out high-pressure pulse electric extraction under the conditions of pulse frequency 250Hz；It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 30min；Enzymolysis solution concentrating under reduced pressure, lyophilization, low-temperature grinding to granularity are that 0.2mm i.e. obtains modified dietary fiber；
Described enzyme is that xylanase, cellulase, laccase, pectase, tannase, amylase and protease 7:6:4:3:2:2:0.4 in mass ratio uniformly mixes.
3. the preparation of fruit vegetable powder
The mass percent of described fruit vegetable powder consists of: Malpighia glabra L. powder 15%, Fructus Citri Limoniae powder 13%, Folium Allii tuberosi powder 10%, celery powder 7%, white gourd powder 7%, Fructus Fragariae Ananssae powder 5%, Radix Dauci Sativae powder 5%, papaya powder 5%, Fructus Ananadis comosi powder 5%, Semen Phaseoli radiati Germinatus powder 5%, orange powder 3%, Citrus powder 3%, Fructus Citri sinensis powder 3%, coconut slurry powder 4%, cranberry powder 3%, cucumber powder 3%, white radish powder 2%, blue berry powder 2%；
Above-mentioned fruit vegetable powder uniformly mixes and i.e. obtains fruit vegetable powder；
The preparation method of each fruit vegetable powder described is: is put by fruit and vegerable in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and cleans 12min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 6mm, the sheet of thickness 4mm or fourth, put in microwave dryer and carry out microwave drying 7min under the conditions of power 3000W, frequency 2000Hz, temperature 55 DEG C, until moisture is down to 6%, then in 25 DEG C, be crushed to granularity 0.2mm, pack and i.e. obtain fruit vegetable powder.
4. the preparation of plant extract
nullThe preparation method of described plant extract is: count by weight，Accurately weigh Jerusalem artichoke 40 parts，Rhizoma Dioscoreae 35 parts，30 parts of Fructus Momordicae charantiae，30 parts of Semen Armeniacae Amarum，Fructus Arctii 25 parts，20 parts of Semen Nelumbinis，Semen Cassiae 18 parts，Bulbus Allii Cepae 12 parts，Germinatus Phragmitis 8 parts，Being crushed to particle diameter is less than 2 millimeters，Then in container, uniformly mix and add the water of 5 times of weight，Control temperature 80 DEG C and keep 3h，Then 50 DEG C it are cooled to，The mixing enzyme preparation adding mixed material gross weight 8% carries out enzymolysis，It is 6.2 with apple vinegar regulation pH value，Enzymolysis 3h，Finally add 1.8 times of w ethanol of mixed material and the mixture of propanol，The mass ratio of ethanol and propanol mixing is 1:2，Control temperature and keep 4h to 70 DEG C，Filter，Filtrate reduced in volume、Lyophilization、Low-temperature grinding is to granularity 0.2mm,Then the vitamin D of ground product quality 0.02% is added,Mix homogeneously i.e. obtains plant extract；
The parts by weight of described mixed enzyme consist of: xylanase 18 parts, pentosanase 18 parts, glucanase 15 parts, lipase 15 parts, pullulanase 10 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucoseoxidase 6 parts, acid phosphatase 8 parts；
Described concentration of alcohol is 75%.
5. the preparation of Folium Camelliae sinensis extract
The preparation method of described Folium Camelliae sinensis extract is: is put by commercially available Folium Camelliae sinensis in the ultrasonic washing unit equipped with 0.8% sodium bicarbonate solution and cleans 15min in 200W, 40KHz, then in the sterile malt dextrin in aqueous solution of 0.30MPa pressure lower surface even application mass percent concentration 1%, Folium Camelliae sinensis with the mass ratio of maltodextrin aqueous solution is: 100:5, seal, rehydration 5h under room temperature；Then in-21 DEG C of freezing 20min；Pulverizing immediately, granularity is 1mm；Folium Camelliae sinensis after pulverizing is placed in pill tank, adds the sterilized water of tea quality 8 times, room temperature 200W, 40KHz condition supersound extraction 15min；Simultaneously in electric field intensity 30KV/cm, burst length 500 μ S, under the conditions of pulse frequency 300Hz, carry out high-pressure pulse electric extraction；Finally by said extracted liquid breast acid for adjusting pH value be 5.5, add mixture quality 1% compound enzyme, in 40 DEG C of low temperature enzymolysis 8h；Enzymolysis solution filtration, filtrate reduced in volume, lyophilization, low-temperature grinding to granularity are that 0.2mm i.e. obtains Folium Camelliae sinensis extract；
Described compound enzyme is uniformly mixed by pectase, cellulase, tannase and protease 1.5:3:3:2 in mass ratio.
6. the preparation of Chinese herbal medicine extract
The preparation method of described Chinese herbal medicine extract is: count by weight, accurately weighs Folium Artemisiae Argyi 53 parts, 52 parts of Bulbus Allii, Herba portulacae 50 parts, Herba Houttuyniae 49 parts, Radix Scutellariae 40 parts, Cortex Phellodendri 35 parts, Fructus Crataegi 35 parts, 30 parts of Radix Glycyrrhizae, Radix Sophorae Flavescentis 20 parts, Rhizoma Alismatis 9 parts, Rhizoma Polygoni Cuspidati 7 parts, Radix Et Rhizoma Rhei 4 parts, Semen Sinapis Albae 3 parts, the Radix Polygoni Multiflori 2 parts；It is less than 2 millimeters that said herbal medicine is crushed to particle diameter respectively, then in container, uniformly mix and add the water of 5 times of weight, control temperature 80 DEG C and keep 3h, be then cooled to 50 DEG C, the compounded enzyme preparate adding mixed material gross weight 8% carries out enzymolysis, it is 6.2 with breast acid for adjusting pH value, enzymolysis 3h, finally adds 1.8 times of w ethanol of mixed material and the mixture of propanol, the mass ratio of ethanol and propanol mixing is 1:2, control temperature and keep 4h to 70 DEG C, filter, obtain the first filtrate；Add the water of 2 times of weight of filtering residue, control temperature 90 DEG C and keep 2h, be then cooled to 30 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate being merged according to mass ratio 2.5:1.5, concentrating under reduced pressure, lyophilization, low-temperature grinding to granularity are that 0.2mm i.e. obtains Chinese herbal medicine extract；
The parts by weight of described compound enzyme consist of: endo-beta-glucanase 20 parts, outer 1,4 beta-glucanase 18 parts, beta-glucosidase 12 parts, xylanase 18 parts, pentosanase 18 parts, pullulanase 25 parts, beta amylase 12 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucoseoxidase 6 parts, acid phosphatase 8 parts, lipase 6 parts；
Described concentration of alcohol is 75%.
Embodiment 2
A kind of fruit and vegerable probiotic tablet, is prepared by the raw material of following parts by weight:
70 parts of probiotic bacteria powder, modified dietary fiber 40 parts, fruit vegetable powder 30 parts, oligosaccharide 25 parts, plant extract 28 parts, Yoghourt powder 22 parts, Folium Camelliae sinensis extract 18 parts, Chinese herbal medicine extract 9 parts, sweeting agent 4 parts, magnesium stearate 0.3 part；
Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 50 parts, bifidobacterium longum 40 parts, bifidobacterium bifidum 30 parts, bifidobacterium infantis 30 parts, bifidobacterium adolescentis 18 parts, Lactobacillus bulgaricus 18 parts, bacillus acidophilus 12 parts, lactobacillus casei 9 parts, streptococcus thermophilus 9 parts, saccharomyces cerevisiae 8 parts, Candida utilis 5 parts；Above-mentioned in addition to Lactobacillus plantarum powder, other is commercial commodity, and viable bacteria content is: 9x1012cfu/g；
Described Lactobacillus plantarum powder, modified dietary fiber, fruit vegetable powder, plant extract, Folium Camelliae sinensis extract, Chinese herbal medicine extract are embodiment 1 to be prepared；
Described oligosaccharide parts by weight consist of oligofructose 45 parts, 35 parts of stachyose, Raffinose 30 parts, oligomeric xylose 17 parts, oligomeric galactose 12 parts, soybean oligo saccharide 12 parts, oligomeric isomaltose 12 parts, Oligomeric maltose 10 parts；
Described Yoghourt powder is commercial commodity, and viable lactic acid bacteria content is 5x1011cfu/g；
Described sweeting agent is that erythritol, xylitol, aspartame are with arbitrary proportion mixing；
The preparation method of described fruit and vegerable probiotic tablet, comprise the steps: accurately to weigh each component raw material by above-mentioned formula, first probiotic bacteria powder and modified dietary fiber are uniformly mixed 25min, then Chinese herbal medicine extract it is sequentially added into, Folium Camelliae sinensis extract, egg albumen powder, plant extract, oligosaccharide, fruit vegetable powder uniformly mixes 12min, add mixture into dry granulating machine pelletize, collect the granule of 15-50 mesh, it is sequentially added into sweeting agent, the raw material of directly compressible is obtained after magnesium stearate mixing, put into tabletting machine, regulation pressure is 30kn, obtain fruit and vegerable probiotic tablet, airtight preservation at a temperature of 25 DEG C, conventionally detect in tablet viable count after placing 12 months and to calculate survival rate be 89%.
Embodiment 3
A kind of fruit and vegerable probiotic tablet, is prepared by the raw material of following parts by weight:
60 parts of probiotic bacteria powder, modified dietary fiber 35 parts, fruit vegetable powder 25 parts, oligosaccharide 20 parts, plant extract 25 parts, egg albumen powder 18 parts, Folium Camelliae sinensis extract 15 parts, Chinese herbal medicine extract 8 parts, sweeting agent 2 parts, magnesium stearate 0.2 part；
Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 45 parts, bifidobacterium longum 35 parts, bifidobacterium bifidum 25 parts, bifidobacterium infantis 25 parts, bifidobacterium adolescentis 15 parts, Lactobacillus bulgaricus 15 parts, bacillus acidophilus 10 parts, lactobacillus casei 8 parts, streptococcus thermophilus 8 parts, saccharomyces cerevisiae 5 parts, Candida utilis 4 parts；Above-mentioned in addition to Lactobacillus plantarum powder, other is commercial commodity, and viable bacteria content is: 8x1012cfu/g；
Described Lactobacillus plantarum powder, modified dietary fiber, fruit vegetable powder, plant extract, Folium Camelliae sinensis extract, Chinese herbal medicine extract are embodiment 1 to be prepared；
Preferably, described oligosaccharide parts by weight consist of oligofructose 40 parts, 30 parts of stachyose, Raffinose 20 parts, oligomeric xylose 16 parts, oligomeric galactose 10 parts, soybean oligo saccharide 10 parts, oligomeric isomaltose 10 parts, Oligomeric maltose 8 parts；
Described egg albumen powder percentage by weight consists of: Yoghourt powder 40%, lactalbumin 35%, soybean protein 15%, casein 10%；
Described Yoghourt powder is commercial commodity, and viable lactic acid bacteria content is 3x1011cfu/g；
Described sweeting agent is that erythritol, xylitol, maltose alcohol, Sorbitol, stevioside, mogroside, aspartame are with arbitrary proportion mixing；
The preparation method of described fruit and vegerable probiotic tablet with embodiment 2, airtight preservation at a temperature of 25 DEG C, conventionally detect in tablet viable count after placing 12 months and to calculate survival rate be 85%.
Embodiment 4
A kind of fruit and vegerable probiotic tablet, is prepared by the raw material of following parts by weight:
80 parts of probiotic bacteria powder, modified dietary fiber 45 parts, fruit vegetable powder 35 parts, oligosaccharide 30 parts, plant extract 30 parts, egg albumen powder 25 parts, Folium Camelliae sinensis extract 20 parts, Chinese herbal medicine extract 10 parts, sweeting agent 5 parts, magnesium stearate 0.5 part；
Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 55 parts, bifidobacterium longum 45 parts, bifidobacterium bifidum 35 parts, bifidobacterium infantis 35 parts, bifidobacterium adolescentis 20 parts, Lactobacillus bulgaricus 20 parts, bacillus acidophilus 15 parts, lactobacillus casei 10 parts, streptococcus thermophilus 10 parts, saccharomyces cerevisiae 10 parts, Candida utilis 6 parts；Above-mentioned in addition to Lactobacillus plantarum powder, other is commercial commodity, and viable bacteria content is: 7x1012cfu/g；
Described Lactobacillus plantarum powder, modified dietary fiber, fruit vegetable powder, plant extract, Folium Camelliae sinensis extract, Chinese herbal medicine extract are embodiment 1 to be prepared；
Described oligosaccharide parts by weight consist of oligofructose 50 parts, 40 parts of stachyose, Raffinose 40 parts, oligomeric xylose 18 parts, oligomeric galactose 15 parts, soybean oligo saccharide 15 parts, oligomeric isomaltose 15 parts, Oligomeric maltose 12 parts；
Described egg albumen powder percentage by weight consists of: Yoghourt powder 40%, lactalbumin 35%, soybean protein 15%, casein 10%；
Described Yoghourt powder is commercial commodity, and viable lactic acid bacteria content is 4x1011cfu/g。
Described sweeting agent is that stevioside, mogroside, aspartame are with arbitrary proportion mixing；
The preparation method of described fruit and vegerable probiotic tablet with embodiment 2, airtight preservation at a temperature of 25 DEG C, conventionally detect in tablet viable count after placing 12 months and to calculate survival rate be 85%.
Embodiment 5
A kind of fruit and vegerable probiotic tablet, is prepared by the raw material of following parts by weight:
80 parts of probiotic bacteria powder, modified dietary fiber 35 parts, fruit vegetable powder 35 parts, oligofructose 20 parts, plant extract 30 parts, lactalbumin 18 parts, Folium Camelliae sinensis extract 20 parts, Chinese herbal medicine extract 10 parts, aspartame 2 parts, magnesium stearate 0.5 part；
Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 55 parts, bifidobacterium longum 35 parts, bifidobacterium bifidum 35 parts, bifidobacterium infantis 25 parts, bifidobacterium adolescentis 20 parts, Lactobacillus bulgaricus 15 parts, bacillus acidophilus 15 parts, lactobacillus casei 10 parts, streptococcus thermophilus 10 parts, saccharomyces cerevisiae 10 parts, Candida utilis 6 parts；Above-mentioned in addition to Lactobacillus plantarum powder, other is commercial commodity, and viable bacteria content is: 9x1012cfu/g；
Described Lactobacillus plantarum powder, modified dietary fiber, fruit vegetable powder, plant extract, Folium Camelliae sinensis extract, Chinese herbal medicine extract are embodiment 1 to be prepared；
The preparation method of described fruit and vegerable probiotic tablet, with embodiment 2 airtight preservation at a temperature of 25 DEG C, conventionally detects in tablet viable count and to calculate survival rate is 83% after placing 12 months.
The probiotic test of embodiment 6 fruit and vegerable probiotic tablet
The fruit and vegerable probiotic tablet embodiment of the present invention 2 prepared, being prepared as viable count with sterilized water is 2 × 1010The probiotic solution of CFU/mL, saves backup in 4 DEG C；
1, the 10mL probiotic solution kept of going bail for is injected in test tube 1, uses ten times of stepwise dilutions to 10-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured on flat board (sterilizing), shake up rapidly after sterilizing.The test tube 2 that will be equipped with 10mL probiotic solution again is placed in 80-90 DEG C of water-bath heating 15-25min, takes the probiotic solution after heating and carries out ten times of stepwise dilutions to 10-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured into upper at flat board (sterilizing) and shake up rapidly after sterilizing.Finally the flat board before heating and after heating is all cultivated under the conditions of 35 DEG C 24h, calculates the quantity before and after heating.
Result shows, Viable detection has reached 88%.
2, simulated gastric fluid and the resistance test of intestinal juice: the hydrochloric acid 16.4mL taking 100g/L adds distilled water diluting, pH value is made to be respectively 1.5,2.5 and 3.5, take 100mL dilute hydrochloric acid solution, it is separately added into 1g pepsin, it is made fully to dissolve, obtaining simulated gastric fluid, microporous filter membrane degerming (0.22 μm) is standby.Taking potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, with 0.1moL/L sodium hydroxide solution regulation pH value to 6.8；Separately taking trypsin 10g, the 100mL that adds water makes dissolving, after two liquid mixing, is diluted with water to 1000ml, obtains simulated intestinal fluid, and microporous filter membrane degerming (0.22 μm) is standby.Take the probiotic solution that 1mL keeps to join in the simulated gastric fluid of 9mL (i.e. ten times stepwise dilutions), and the most fully mix rapidly, be subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Taking out culture fluid respectively 1h, 2h, 3h, 4h when and count remaining viable count immediately, comparing with former viable count, result shows, Viable detection is 97%.Then each 1mL of culture fluid digesting different time it is taken in simulated gastric fluid, it is inoculated in respectively in the simulated intestinal fluid that 9mL pH value is 6.8, it is placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h sampling, measure its viable count, comparing with former viable count, result shows that Viable detection is 99%.
3, simulating the resistance test of cholate: make the solution of 1g/L with pancreatin, and add the Fel Sus domestica salt of 0.8% in the solution, the NaOH adjustment pH with 10% is 8.0, then with 0.45 μm micro-filtrate membrane filtration degerming.The probiotic solution kept by 0.5mL is inoculated in 4.5mL simulation cholate, obtains culture fluid, the viable count of counting remaining after cultivating 24h.By culture fluid in sterile saline ten times of stepwise dilutions to 10-8, and pour into MRS, it is subsequently placed in 35 DEG C of quiescent culture 24h.Result shows that Viable detection is 99%.
Embodiment 7 fruit and vegerable probiotics tablets mouse intestinal performance test
The fruit and vegerable probiotic tablet embodiment of the present invention 2 prepared, being prepared as viable count with sterilized water is 2 × 1010The probiotic solution of CFU/mL, saves backup in 4 DEG C；
Choosing common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40,9:00 gavage lincomycin hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepare the mouse model of alteration of intestinal flora.Model group mouse diet declines, and dead and phenomenon of significantly suffering from diarrhoea does not occurs, arranges soft excrement, and profile normal aqueous divides more, and bedding and padding are moist.By 40 alteration of intestinal flora mices, being randomly divided into 2 groups, one group 20 is only used as treatment group, the probiotic solution 0.5ml (2 × 10 that every day, gavage was kept10Cfu/ml)/only, another 20 are only used as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observe growth and the defecation situation of white mice every day, weigh in the 8th, 21 days mices to probiotic tablet treatment group and natural recovering group, calculate each group of weight average rate of increase, result such as table 1；Within every 5 days, survey each group of stool in mice escherichia coli quantity, calculate average, result such as table 2.Take stool in mice about 0.1g, in aseptic operating platform add 3 beades (adding 0.5mL diluent with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram wet just in coliform count.
Table 1 mice Gain weight
Packet Average starting weight (g/ is only) Average end weight (g/ is only) Average rate of increase (%)
Natural recovering group 20.69±1.33 27.34±1.59 32.14a
Probiotic tablet treatment group 20.41±1.45 31.29±1.78 53.31b
The situation of coliform count in table 2 stool in mice
Probiotic tablet treatment group Mouse Weight average rate of increase (53.31%) is significantly higher than natural recovering group (32.14%)；Feed solution rear intestinal escherichia coli quantity to be remarkably decreased, reduce by 80.89%, it is substantially less than natural recovering group (24.78%), show the rapid field planting in white mice intestinal of the probiotic bacteria in fruit and vegerable probiotic tablet of the present invention, form dominant microflora, and the growth and breeding of effective suppression pathogen such as escherichia coli, and resident time is long, continues, effectively improves intestinal performance.
Embodiment 8 fruit and vegerable of the present invention probiotic tablet judge experiment
The fruit and vegerable probiotic tablet inviting 24 people to prepare embodiment of the present invention 2-5 is judged with commercially available two kinds of probiotic tablets, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), and marking personnel independently carry out, are independent of each other, and judges result with guarantee accurate.Being added up judging result, equal score value takes approximation, retains integer, is specifically shown in Table 3:
Table 3 sensory evaluation statistical result
Note: show significant difference (P ＜ 0.05) with a line internal standard difference lowercase alphabet, the different capitalization of mark represents that difference is extremely notable (P ＜ 0.01), indicates same letter and represents that difference is not notable (P ＞ 0.05).
Result above shows, any one will be substantially better than commercially available common probiotic tablet to fruit and vegerable probiotic tablet prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, also is adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.
The fat-reducing effect of embodiment 9 fruit and vegerable of the present invention probiotic tablet
Select the nutrition probiotic tablet that the overweight people of 100 different age group uses the embodiment of the present invention 2 to prepare at random, product specification: 32 x 0.4g；Same age bracket men and women half and half, wherein Mild Obesity person 56, central obesity person 32, severe obesity 12, morning, noon and afternoon every day three times, each 1.2g, warm boiled water, 30 days is a cycle.Taking one week after date continuously, experimenter's mental status generally makes moderate progress, and all testing crews reflection anxiety significantly reduces；Well sleep quality is had than before experiment.Through health examination, the every health indicator of health is superior to the sign situation before test, and, experimenter's self-control strengthens, autonomic movement time lengthening, and life, study and work more have regularity, enter eucyclic weight loss procedures.Experimenter's body weight is detected simultaneously, be the results are shown in Table 4, table 5.
Table 4 different age group body weight testing result
Body weight change Increase more than 1kg Within increase and decrease 1kg Reduce 1-5kg Reduce 5kg-10kg Reduce more than 10kg Add up to
Under-18s 1 3 9 4 1 18
18-25 year 0 1 9 6 4 20
25-30 year 0 0 8 7 5 20
30-50 year 0 0 12 5 4 21
More than 50 years old 0 0 15 4 2 21
Add up to 1 4 53 26 16 100
Result above shows, fruit and vegerable probiotic tablet of the present invention all has remarkable result to the men and women crowd of different age group, take a cycle (30 days), the number reducing more than 1kg is 95 people, fat-reducing effect has reached more than 95%, wherein reduces 1-5kg, 5-10kg, more than 10kg respectively 53%, 26% and 16%；The more significant age bracket of fat-reducing effect be 25-50 year more than, wherein reduce the crowd of more than 10kg be mainly 25-30 year, be secondly 30-50 year crowd；The population of weight of more than 50 years old reduces main at below 10kg, and in a word, fruit and vegerable probiotic tablet of the present invention can be suitable for the overweight people of various all ages and classes and eat, and has significant fat-reducing effect.
The different obesity phenotype body weight testing result of table 5
Body weight change Increase more than 1kg Within increase and decrease 1kg Reduce 1-5kg Reduce 5kg-10kg Reduce more than 10kg Add up to
Slightly 1 1 36 10 4 52
Moderate 0 3 14 10 6 33
Seriously 0 0 3 6 6 15
Add up to 1 4 53 26 16 100
Result above shows, the severe obesity person reducing more than 10kg accounts for 37.5%, and central obesity person accounts for 37.5%, and Mild Obesity person accounts for 25%；The severe obesity person reducing 1-5kg accounts for 5.66%, and central obesity person accounts for 26.42%, and Mild Obesity person accounts for 67.92%, the fat-reducing effect of fruit and vegerable probiotic tablet alignment degree of the present invention and severe obesity person is described more preferably.

Claims (9)

1. a fruit and vegerable probiotic tablet, is prepared by the raw material of following parts by weight: probiotic bacteria powder 60-80 part, modified dietary fiber 35-45 part, fruit vegetable powder 25-35 part, oligosaccharide 20-30 part, plant extract 25-30 part, egg albumen powder 18-25 part, tea Leaf extract 15-20 part, Chinese herbal medicine extract 8-10 part, sweeting agent 2-5 part, magnesium stearate 0.2-0.5 part；
Described modified fibre is to be obtained through enzyme enzymolysis by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber, Testa oryzae, wheat bran Arrive；
The preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber, Testa oryzae, wheat bran 7-12:6-9:3-5:2-4:1-3:1-2 in mass ratio uniformly mixes, and adds the water of its quality 3-7 times, room temperature 200-300W, 35-40KHz condition supersound extraction 10-15min, simultaneously in electric field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under the conditions of pulse frequency 200-300Hz；It is 4.5-6.5 with breast acid for adjusting pH value, Add the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution concentrating under reduced pressure, lyophilization, Low-temperature grinding to granularity is that 0.1-0.3mm i.e. obtains modified dietary fiber；
Described enzyme be xylanase, cellulase, laccase, pectase, tannase, amylase and protease in mass ratio 6-8:4-7:3-5:2-4:1-3:1-3:0.3-0.5 uniformly mixes；
Described plant extract be with can promote lactobacter growth and leptin content higher natural plants extracted and prepared.
2. fruit and vegerable probiotic tablet as claimed in claim 1, it is characterised in that described probiotic bacteria powder is by the powder of following parts by weight Agent is uniformly mixed: Lactobacillus plantarum 45-55 part, bifidobacterium longum 35-45 part, bifidobacterium bifidum 25-35 part, baby Youngster's bifidus bacillus 25-35 part, bifidobacterium adolescentis 15-20 part, Lactobacillus bulgaricus 15-20 part, bacillus acidophilus 10-15 Part, lactobacillus casei 8-10 part, streptococcus thermophilus 8-10 part, saccharomyces cerevisiae 5-10 part, Candida utilis 4-6 part.
3. fruit and vegerable probiotic tablet as claimed in claim 2, it is characterised in that the preparation method of described Lactobacillus plantarum powder includes Following steps: Lactobacillus plantarum CGMCC NO.9405 strain is obtained through one, two, three shaking flask and first class seed pot amplification culture To first class seed pot strain；Accessing secondary seed tank with 5% inoculum concentration, fermentation medium loading amount is 2/3rds that tank holds, cultivates Condition is temperature 37 DEG C, tank pressure 0.05MPa, incubation time 22 hours, and fermentation liquid staticly settles, centrifugal be precipitated thing, so The carrier of rear interpolation precipitate quality 0.8 times, mix homogeneously, 50 DEG C of fluid bed dryings i.e. obtain Lactobacillus plantarum powder, carrier weight Amount consists of: Yoghourt powder 25 parts, 12 parts of dextrin.
4. fruit and vegerable probiotic tablet as claimed in claim 1, it is characterised in that the mass percent of described fruit vegetable powder consists of: pin Leaf cherry powder 15%, Fructus Citri Limoniae powder 13%, Folium Allii tuberosi powder 10%, celery powder 7%, white gourd powder 7%, Fructus Fragariae Ananssae powder 5%, Radix Dauci Sativae powder 5%, papaya powder 5%, Fructus Ananadis comosi powder 5%, Semen Phaseoli radiati Germinatus powder 5%, orange powder 3%, Citrus powder 3%, Fructus Citri sinensis powder 3%, coconut slurry powder 4%, cranberry powder 3%, cucumber powder 3%, white radish powder 2%, blue berry powder 2%；The preparation method of each fruit vegetable powder described For: fruit and vegerable are put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 10-15min, drip in 200W, 40KHz Dry, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, put in microwave dryer in power 3000W, Carry out microwave drying 6-12min under the conditions of frequency 2000Hz, temperature 50-60 DEG C, until moisture is down to 5-8%, then in 20-30 DEG C, be crushed to granularity 0.1-0.3mm, pack and i.e. obtain fruit vegetable powder.
5. fruit and vegerable probiotic tablet as claimed in claim 1, it is characterised in that the preparation method of described plant extract is: by weight Amount number meter, accurately weighs Jerusalem artichoke 30-50 part, Rhizoma Dioscoreae 30-40 part, Fructus Momordicae charantiae 20-40 part, Semen Armeniacae Amarum 20-40 part, Fructus Arctii 20-30 Part, Semen Nelumbinis 15-25 part, Semen Cassiae 15-20 part, Bulbus Allii Cepae 10-15 part, Germinatus Phragmitis 5-10 part, be crushed to particle diameter be 2 millimeters with Under, in container, then uniformly mix and add the water of 5 times of weight of mixture, control temperature 80 DEG C and keep 3h, be then cooled to 50 DEG C, the mixing enzyme preparation adding mixed material gross weight 8% carries out enzymolysis, is 6.2 with apple vinegar regulation pH value, enzymolysis 3h, Finally adding the mixture that 1.8 times of weight concentrations of mixed material are 75% ethanol and propanol, the mass ratio of ethanol and propanol mixing is 1:2, control temperature to 70 DEG C keep 4h, filter, filtrate reduced in volume, lyophilization, low-temperature grinding to granularity 0.1-0.3mm, Then adding the vitamin D of ground product quality 0.01-0.03%, mix homogeneously i.e. obtains plant extract；
The parts by weight of described mixed enzyme consist of: xylanase 18 parts, pentosanase 18 parts, glucanase 15 parts, lipase 15 parts, pullulanase 10 parts, acid protease 12 parts, pectase 12 parts, seminase 12 parts, glucoseoxidase 6 parts, acid Acid phosphatase 8 parts.
6. fruit and vegerable probiotic tablet as claimed in claim 1, it is characterised in that described egg albumen powder percentage by weight consists of: Yoghourt Powder 40%, lactalbumin 35%, soybean protein 15%, casein 10%.
7. fruit and vegerable probiotic tablet as claimed in claim 1, it is characterised in that the preparation method of described Chinese herbal medicine extract is: press Parts by weight meter, accurately weighs Folium Artemisiae Argyi 50-55 part, Bulbus Allii 50-55 part, Herba portulacae 45-55 part, Herba Houttuyniae 45-55 part, Radix Scutellariae 30-45 part, Cortex Phellodendri 30-40 part, Fructus Crataegi 30-40 part, Radix Glycyrrhizae 25-35 part, Radix Sophorae Flavescentis 15-25 part, Rhizoma Alismatis 8-10 part, Rhizoma Polygoni Cuspidati 6-8 part, Radix Et Rhizoma Rhei 2-5 part, Semen Sinapis Albae 2-5 part, Radix Polygoni Multiflori 1-3 part；It is 2 that said herbal medicine is crushed to particle diameter respectively Below Hao meter, in container, then uniformly mix and add the water of 5 times of weight of mixture, control temperature 80 DEG C and keep 3h, then Being cooled to 50 DEG C, the compound enzyme adding mixed material gross weight 8% carries out enzymolysis, is 6.2 with breast acid for adjusting pH value, enzymolysis 3h, Finally add ethanol and the mixture of propanol that 1.8 times of weight concentrations of mixed material are 75%, ethanol and the mass ratio of propanol mixing For 1:2, control temperature and keep 4h to 70 DEG C, filter, obtain the first filtrate；Add the water of 2 times of weight of filtering residue, control temperature 90 DEG C Keep 2h, be then cooled to 30 DEG C, filter, obtain the second filtrate；By the first filtrate and the second filtrate according to mass ratio 2.5:1.5 Merging, concentrating under reduced pressure, lyophilization, low-temperature grinding to granularity are that 0.1-0.3mm i.e. obtains Chinese herbal medicine extract；
The parts by weight of described compound enzyme consist of: endo-beta-glucanase 20 parts, outer 1,4 beta-glucanase 18 parts, beta-glucosidase Enzyme 12 parts, xylanase 18 parts, pentosanase 18 parts, pullulanase 25 parts, beta amylase 12 parts, acid protease 12 parts, Pectase 12 parts, seminase 12 parts, glucoseoxidase 6 parts, acid phosphatase 8 parts, lipase 6 parts.
8. the preparation method of fruit and vegerable probiotic tablet as described in claim 1-7 is arbitrary, it is characterised in that comprise the steps: according to Formula accurately weighs each component raw material, first probiotic bacteria powder and modified dietary fiber is uniformly mixed 20-30min, the most successively Add Chinese herbal medicine extract, Folium Camelliae sinensis extract, egg albumen powder, plant extract, oligosaccharide, fruit vegetable powder and uniformly mix 10-15min, Add mixture into dry granulating machine pelletize, collect the granule of 10-100 mesh, obtain after being sequentially added into sweeting agent, magnesium stearate mixing To the raw material of directly compressible, putting into tabletting machine, regulation pressure is 10-40kn, obtains fruit and vegerable probiotic tablet.
9. the preparation method of fruit and vegerable probiotic tablet as claimed in claim 8, it is characterised in that the preparation method of described Folium Camelliae sinensis extract In turn include the following steps: commercially available Folium Camelliae sinensis is put in the ultrasonic washing unit equipped with 0.8% sodium bicarbonate solution in 200W, 40KHz Clean 10-15min, then aseptic in 0.25-0.35MPa pressure lower surface even application mass percent concentration 0.5-1.5% Maltodextrin aqueous solution, Folium Camelliae sinensis with the mass ratio of maltodextrin aqueous solution is: 100:0.5-15, seals, rehydration 2-8h under room temperature； Then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, granularity is 0.1-2mm；Folium Camelliae sinensis after pulverizing is placed in dispensing In cylinder, add the sterilized water of tea quality 5-10 times, room temperature 200W, 40KHz condition supersound extraction 10-15min；Simultaneously in Electric field intensity 20-40kV/cm, burst length 400-500 μ s, carry out high-pressure pulse electric under the conditions of pulse frequency 200-300Hz and carry Take；Finally by said extracted liquid breast acid for adjusting pH value be 4.5-6.5, add mixture quality 0.5-1.5% compound enzyme, in 35-45 DEG C of low temperature enzymolysis 4-12h；Enzymolysis solution filtration, filtrate reduced in volume, lyophilization, low-temperature grinding to granularity are 0.1-0.3mm Obtain Folium Camelliae sinensis extract；
Described compound enzyme is that pectase, cellulase, tannase, protease 1-2:2-4:2-4:1-3 in mass ratio uniformly mixes.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1192360A (en) * 1997-08-01 1998-09-09 北京东方百信生物技术有限公司 Medicinal prepn. contg. beneficial bacteria named 'Junyikang' and method for preparing same
CA2551581A1 (en) * 2003-12-24 2005-07-14 Ferrosan A/S Probiotic tablet formulations
CN103704423A (en) * 2014-01-02 2014-04-09 绿锦发展(福建)有限公司 Preparation method for tea extract
CN104095180A (en) * 2013-04-12 2014-10-15 中国科学院烟台海岸带研究所 Probiotics inulin sheet and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1192360A (en) * 1997-08-01 1998-09-09 北京东方百信生物技术有限公司 Medicinal prepn. contg. beneficial bacteria named 'Junyikang' and method for preparing same
CA2551581A1 (en) * 2003-12-24 2005-07-14 Ferrosan A/S Probiotic tablet formulations
CN104095180A (en) * 2013-04-12 2014-10-15 中国科学院烟台海岸带研究所 Probiotics inulin sheet and preparation method thereof
CN103704423A (en) * 2014-01-02 2014-04-09 绿锦发展(福建)有限公司 Preparation method for tea extract

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